WO1999043845A3 - Polynucleotide sequencing method and kits therefor - Google Patents

Polynucleotide sequencing method and kits therefor Download PDF

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Publication number
WO1999043845A3
WO1999043845A3 PCT/GB1999/000539 GB9900539W WO9943845A3 WO 1999043845 A3 WO1999043845 A3 WO 1999043845A3 GB 9900539 W GB9900539 W GB 9900539W WO 9943845 A3 WO9943845 A3 WO 9943845A3
Authority
WO
WIPO (PCT)
Prior art keywords
sequencing method
polynucleotide
polynucleotide sequencing
kits therefor
site
Prior art date
Application number
PCT/GB1999/000539
Other languages
French (fr)
Other versions
WO1999043845A2 (en
Inventor
Miles Douglas Houslay
Neil Graham Rena
Original Assignee
Univ Glasgow
Houslay Miles D
Rena Neil G
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Glasgow, Houslay Miles D, Rena Neil G filed Critical Univ Glasgow
Priority to AU32592/99A priority Critical patent/AU3259299A/en
Publication of WO1999043845A2 publication Critical patent/WO1999043845A2/en
Publication of WO1999043845A3 publication Critical patent/WO1999043845A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a method of sequencing a polynucleotide utilising restriction enzymes which cleave the polynucleotide at a site away from the restriction enzyme's recognition site and generate a cleaved site possessing a recessed 3'-end and a 5'-overhang of undefined sequence, as well as to kits and a computer program for use with such a method.
PCT/GB1999/000539 1998-02-26 1999-02-26 Polynucleotide sequencing method and kits therefor WO1999043845A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU32592/99A AU3259299A (en) 1998-02-26 1999-02-26 Polynucleotide sequencing method and kits therefor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9803930.8A GB9803930D0 (en) 1998-02-26 1998-02-26 Polynucleotide sequencing method and kits therefor
GB9803930.8 1998-02-26

Publications (2)

Publication Number Publication Date
WO1999043845A2 WO1999043845A2 (en) 1999-09-02
WO1999043845A3 true WO1999043845A3 (en) 2000-03-09

Family

ID=10827545

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1999/000539 WO1999043845A2 (en) 1998-02-26 1999-02-26 Polynucleotide sequencing method and kits therefor

Country Status (3)

Country Link
AU (1) AU3259299A (en)
GB (1) GB9803930D0 (en)
WO (1) WO1999043845A2 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0630972A2 (en) * 1993-06-25 1994-12-28 Hitachi, Ltd. DNA analyzing method
WO1995027080A2 (en) * 1994-04-04 1995-10-12 Lynx Therapeutics Inc Dna sequencing by stepwise ligation and cleavage
EP0767240A2 (en) * 1995-09-18 1997-04-09 Hitachi, Ltd. DNA sequencing method and DNA sample preparation method
US5710000A (en) * 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0630972A2 (en) * 1993-06-25 1994-12-28 Hitachi, Ltd. DNA analyzing method
WO1995027080A2 (en) * 1994-04-04 1995-10-12 Lynx Therapeutics Inc Dna sequencing by stepwise ligation and cleavage
US5710000A (en) * 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
EP0767240A2 (en) * 1995-09-18 1997-04-09 Hitachi, Ltd. DNA sequencing method and DNA sample preparation method

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BRENNER S ET AL: "DNA FINGERPRINTING BY SAMPLED SEQUENCING", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,US,NATIONAL ACADEMY OF SCIENCE. WASHINGTON, vol. 86, pages 8902-8906, XP000606224, ISSN: 0027-8424 *
UNRAU P. ET AL.: "Non-cloning amplification of specific DNA fragments from whole genomic DNA digests using DNA "indexers"", GENE (ISSN: 0378-1119), vol. 145, 1994, pages 163 - 169, XP002056703 *

Also Published As

Publication number Publication date
AU3259299A (en) 1999-09-15
WO1999043845A2 (en) 1999-09-02
GB9803930D0 (en) 1998-04-22

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