WO1999043783A3 - TRANSGENIC MOUSE HAVING A LacZ REPORTER GENE UNDER THE CONTROL OF THE N-CAM PROMOTER - Google Patents

TRANSGENIC MOUSE HAVING A LacZ REPORTER GENE UNDER THE CONTROL OF THE N-CAM PROMOTER Download PDF

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Publication number
WO1999043783A3
WO1999043783A3 PCT/US1999/004118 US9904118W WO9943783A3 WO 1999043783 A3 WO1999043783 A3 WO 1999043783A3 US 9904118 W US9904118 W US 9904118W WO 9943783 A3 WO9943783 A3 WO 9943783A3
Authority
WO
WIPO (PCT)
Prior art keywords
reporter gene
transgenic mouse
control
cam
gene under
Prior art date
Application number
PCT/US1999/004118
Other languages
French (fr)
Other versions
WO1999043783A2 (en
Inventor
Kathryn L Crossin
Gerald M Edelman
Brent D Holst
Original Assignee
Scripps Research Inst
Kathryn L Crossin
Gerald M Edelman
Brent D Holst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst, Kathryn L Crossin, Gerald M Edelman, Brent D Holst filed Critical Scripps Research Inst
Priority to EP99908469A priority Critical patent/EP1182924A4/en
Publication of WO1999043783A2 publication Critical patent/WO1999043783A2/en
Publication of WO1999043783A3 publication Critical patent/WO1999043783A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0393—Animal model comprising a reporter system for screening tests

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Physics & Mathematics (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention describes a transgenic mouse having a knockout N-CAM allele with an insertion of a reporter gene under the control of the N-CAM promoter. In preferred embodiments, the reporter gene encodes beta-galactosidase. The invention also describes methods for detecting modulators of the N-CAM gene using the transgenic mouse, or cultured cells obtained from the mouse.
PCT/US1999/004118 1998-02-25 1999-02-25 TRANSGENIC MOUSE HAVING A LacZ REPORTER GENE UNDER THE CONTROL OF THE N-CAM PROMOTER WO1999043783A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP99908469A EP1182924A4 (en) 1998-02-25 1999-02-25 TRANSGENIC MOUSE HAVING A LacZ REPORTER GENE UNDER THE CONTROL OF THE N-CAM PROMOTER USEFUL FOR IDENTIFYING N-CAM MODULATORS, METHODS AND COMPOSITIONS

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7598598P 1998-02-25 1998-02-25
US60/075,985 1998-02-25

Publications (2)

Publication Number Publication Date
WO1999043783A2 WO1999043783A2 (en) 1999-09-02
WO1999043783A3 true WO1999043783A3 (en) 2001-12-13

Family

ID=22129195

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/004118 WO1999043783A2 (en) 1998-02-25 1999-02-25 TRANSGENIC MOUSE HAVING A LacZ REPORTER GENE UNDER THE CONTROL OF THE N-CAM PROMOTER

Country Status (2)

Country Link
EP (1) EP1182924A4 (en)
WO (1) WO1999043783A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9925125D0 (en) 1999-10-22 1999-12-22 Novartis Ag Organic compounds
AU2002239539A1 (en) * 2000-12-06 2002-06-18 Deltagen, Inc. Transgenic mice containing targeted gene disruptions
US8952213B2 (en) 2002-04-26 2015-02-10 The Board Of Trustees Of The Leland Stanford Junior University Neuronal activation in a transgenic model
US7425618B2 (en) 2002-06-14 2008-09-16 Medimmune, Inc. Stabilized anti-respiratory syncytial virus (RSV) antibody formulations

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5792743A (en) * 1995-04-19 1998-08-11 Acorda Therapeutics Method for promoting neural growth comprising administering a soluble neural cell adhesion molecule

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5792743A (en) * 1995-04-19 1998-08-11 Acorda Therapeutics Method for promoting neural growth comprising administering a soluble neural cell adhesion molecule

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
CONQUER F.: "Inactivation in vivo of metabotropic glutamate receptor 1 by specific chromosomal insertion of reporter gene lacz", NEUROPHARMACOLOGY, vol. 34, no. 8, 1995, pages 865 - 870, XP002922616 *
CREMER H. ET AL.: "Inactivation of the N-CAM gene in mice results in size reduction of the olfactory bulb and dificits in the spatial learning", NATURE, vol. 367, 3 February 1994 (1994-02-03), pages 455 - 459, XP002922617 *
CREMER H. ET AL.: "Long-term but not short-term plasticity at mossy fiber synapses is impaired in neural cell adhesion molecule-deficient mice", PROC. NATL. ACAD. SCI. USA, vol. 95, October 1998 (1998-10-01), pages 13242 - 13247, XP002922615 *
HOLST B.D. ET AL.: "Allosteric modulation of a AMPA-type glutamate receptors increases activity of the promoter for the neural cell adhesion molecular, N-CAM", PROC. NATL. ACAD. SCI. USA, March 1998 (1998-03-01), pages 2597 - 2602, XP002922604 *
See also references of EP1182924A4 *
TOMASIEWICZ H. ET AL.: "Genetic deletion of a neural cell adhesion molecule variant (N-CAM-180) produces distinct defects in the central nervous system", NEURON., vol. 11, December 1993 (1993-12-01), pages 1163 - 1174, XP002922618 *
WOOD G.K. ET AL.: "NCAM-180 knockout mice display increased lateral ventricle size and reduced prepulse inhibition of startle", NEUROREPORT, vol. 9, no. 3, 16 February 1998 (1998-02-16), pages 461 - 466, XP002922619 *

Also Published As

Publication number Publication date
EP1182924A2 (en) 2002-03-06
WO1999043783A2 (en) 1999-09-02
EP1182924A4 (en) 2003-01-02

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