WO1999038956A3 - Enzyme modifying rhamnogalacturonane ii, dna encoding for said enzymes and method for producing the enzyme - Google Patents

Enzyme modifying rhamnogalacturonane ii, dna encoding for said enzymes and method for producing the enzyme Download PDF

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Publication number
WO1999038956A3
WO1999038956A3 PCT/EP1999/000619 EP9900619W WO9938956A3 WO 1999038956 A3 WO1999038956 A3 WO 1999038956A3 EP 9900619 W EP9900619 W EP 9900619W WO 9938956 A3 WO9938956 A3 WO 9938956A3
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WO
WIPO (PCT)
Prior art keywords
enzyme
modifying
gly
tyr
ala
Prior art date
Application number
PCT/EP1999/000619
Other languages
French (fr)
Other versions
WO1999038956A2 (en
Inventor
Stephane Vidal
Petrus Jacobus Theodoru Dekker
Patrice Jacques Marie Pellerin
Original Assignee
Agronomique Inst Nat Rech
Dsm Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agronomique Inst Nat Rech, Dsm Nv filed Critical Agronomique Inst Nat Rech
Publication of WO1999038956A2 publication Critical patent/WO1999038956A2/en
Publication of WO1999038956A3 publication Critical patent/WO1999038956A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01171—Rhamnogalacturonan hydrolase (3.2.1.171), i.e. rhamnogalacturonase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention provides an enzyme in substantially pure form, having Rhamnogalacturonan-II modifying activity. A preferred enzyme according to the invention is obtainable from Penicillium daleae. More in particular, the enzymatic activity is a degrading activity (with degrading is meant reducing the polymerisation state). According to a more preferred embodiment, the enzyme comprises fragments having an amino acid sequence selected from the group consisting of: (a) Lys Pro Tyr Gly Asp His Gly Val Trp Val Gln Asn Ala Ile Gly Leu Leu His Glu Gly Gly Gln Tyr Tyr; (b) Val Ser Gly Trp Thr Ala Asn Asp Asp Gly Ile Tyr Val Ala Ser Val Pro Glu; and (c) Asn Leu Tyr Ile Asp Gly Gln Ala Ala Asn Tyr Ala Arg. A particularly preferred enzyme has the amino acid sequence as represented in SEQ ID NO: 1, or a fragment or variant thereof which has rhamnogalacturonan-II modifying properties. According to a preferred embodiment of the invention, the RG-II modifying enzyme is a rhamnogalacturonan-II hydrolase. Also provided are DNA sequences coding for an RG-II modifying enzyme, transformed host cells capable of expressing said DNA sequences and methods for producing an RG-II modifying enzyme. The enzyme or compositions of enzyme containing an RG-II modifying enzyme are suitably used in the manufacturing of fruit or vegetable derived products, such as beverages.
PCT/EP1999/000619 1998-01-30 1999-02-01 Enzyme modifying rhamnogalacturonane ii, dna encoding for said enzymes and method for producing the enzyme WO1999038956A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98400207 1998-01-30
EP98400207.1 1998-01-30

Publications (2)

Publication Number Publication Date
WO1999038956A2 WO1999038956A2 (en) 1999-08-05
WO1999038956A3 true WO1999038956A3 (en) 1999-10-21

Family

ID=8235256

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/000619 WO1999038956A2 (en) 1998-01-30 1999-02-01 Enzyme modifying rhamnogalacturonane ii, dna encoding for said enzymes and method for producing the enzyme

Country Status (1)

Country Link
WO (1) WO1999038956A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1339837B1 (en) 2000-12-07 2008-02-13 DSM IP Assets B.V. Prolyl endoprotease from aspergillus niger
AU2002358179A1 (en) 2001-12-21 2003-07-09 Dsm Ip Assets B.V. New rennets
EP1705998B1 (en) * 2004-01-30 2014-05-21 DSM IP Assets B.V. Use of a carboxypeptidase

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992019728A1 (en) * 1991-05-02 1992-11-12 Novo Nordisk A/S Rhamnogalacturonase, corresponding dna sequence, rhamnogalacturonase containing enzyme preparation and use of the enzyme preparation
WO1993020190A1 (en) * 1992-03-27 1993-10-14 Novo Nordisk A/S A novel enzyme and a dna sequence
EP0570075A2 (en) * 1992-05-15 1993-11-18 Quest International B.V. Cloning and expression of DNA encoding a ripening form of a polypeptide having rhamnogalacturonase activity
WO1994020612A1 (en) * 1993-03-05 1994-09-15 Novo Nordisk A/S An enzyme with rhamnogalacturonase activity
WO1996006163A1 (en) * 1994-08-18 1996-02-29 Novo Nordisk A/S Novel rhamnogalacturonan rhamnosidases
WO1996037604A2 (en) * 1995-05-23 1996-11-28 Institut National De La Recherche Agronomique Enzyme and microorganism degrading rhamnogalacturonane ii

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992019728A1 (en) * 1991-05-02 1992-11-12 Novo Nordisk A/S Rhamnogalacturonase, corresponding dna sequence, rhamnogalacturonase containing enzyme preparation and use of the enzyme preparation
WO1993020190A1 (en) * 1992-03-27 1993-10-14 Novo Nordisk A/S A novel enzyme and a dna sequence
EP0570075A2 (en) * 1992-05-15 1993-11-18 Quest International B.V. Cloning and expression of DNA encoding a ripening form of a polypeptide having rhamnogalacturonase activity
WO1994020612A1 (en) * 1993-03-05 1994-09-15 Novo Nordisk A/S An enzyme with rhamnogalacturonase activity
WO1996006163A1 (en) * 1994-08-18 1996-02-29 Novo Nordisk A/S Novel rhamnogalacturonan rhamnosidases
WO1996037604A2 (en) * 1995-05-23 1996-11-28 Institut National De La Recherche Agronomique Enzyme and microorganism degrading rhamnogalacturonane ii

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
PELLERIN P ET AL: "Structural characterization of red wine rhamnogalacturonan II", CARBOHYDRATE RESEARCH., vol. 290, no. 2, 2 September 1996 (1996-09-02), AMSTERDAM NL, pages 183-197, XP004018931 *
SCHOLS H A ET AL: "RHAMNOGALACTURONASE: A NOVEL ENZYME THAT DEGRADES THE HAIRY REGIONS OF PECTINS*", CARBOHYDRATE RESEARCH., vol. 206, no. 1, 30 September 1990 (1990-09-30), AMSTERDAM NL, pages 105 - 115, XP000257186 *

Also Published As

Publication number Publication date
WO1999038956A2 (en) 1999-08-05

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