WO1999037755A3 - The use of consensus sequences for targeted homologous gene isolation and recombination in gene families - Google Patents

The use of consensus sequences for targeted homologous gene isolation and recombination in gene families Download PDF

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Publication number
WO1999037755A3
WO1999037755A3 PCT/US1998/026498 US9826498W WO9937755A3 WO 1999037755 A3 WO1999037755 A3 WO 1999037755A3 US 9826498 W US9826498 W US 9826498W WO 9937755 A3 WO9937755 A3 WO 9937755A3
Authority
WO
WIPO (PCT)
Prior art keywords
recombination
gene
consensus sequences
targeted homologous
families
Prior art date
Application number
PCT/US1998/026498
Other languages
French (fr)
Other versions
WO1999037755A2 (en
Inventor
Sushma Pati
David Zarling
Christopher W Lehman
Hong Zeng
Original Assignee
Pangene Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pangene Corporation filed Critical Pangene Corporation
Priority to JP2000528663A priority Critical patent/JP2002500882A/en
Priority to AU19135/99A priority patent/AU762766B2/en
Priority to EP98963901A priority patent/EP1038017A2/en
Priority to CA002314246A priority patent/CA2314246A1/en
Publication of WO1999037755A2 publication Critical patent/WO1999037755A2/en
Publication of WO1999037755A3 publication Critical patent/WO1999037755A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention relates to compositions and methods for targeting sequence modifications in one or more genes of a related family of genes using enhanced homologous recombination techniques. The invention also relates to compositions and methods for isolating and identifying novel members of homologous sequence families. These techniques may be used to create animal or plant models of disease as well as to identify new targets for drug or pathogen screening.
PCT/US1998/026498 1997-12-11 1998-12-11 The use of consensus sequences for targeted homologous gene isolation and recombination in gene families WO1999037755A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2000528663A JP2002500882A (en) 1997-12-11 1998-12-11 Use of consensus sequences for isolation and recombination of target homologous genes in gene families
AU19135/99A AU762766B2 (en) 1997-12-11 1998-12-11 The use of consensus sequences for targeted homologous gene isolation and recombination in gene families
EP98963901A EP1038017A2 (en) 1997-12-11 1998-12-11 The use of consensus sequences for targeted homologous gene isolation and recombination in gene families
CA002314246A CA2314246A1 (en) 1997-12-11 1998-12-11 The use of consensus sequences for targeted homologous gene isolation and recombination in gene families

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7073497P 1997-12-11 1997-12-11
US60/070,734 1997-12-11

Publications (2)

Publication Number Publication Date
WO1999037755A2 WO1999037755A2 (en) 1999-07-29
WO1999037755A3 true WO1999037755A3 (en) 1999-12-29

Family

ID=22097063

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/026498 WO1999037755A2 (en) 1997-12-11 1998-12-11 The use of consensus sequences for targeted homologous gene isolation and recombination in gene families

Country Status (6)

Country Link
US (2) US6524856B1 (en)
EP (1) EP1038017A2 (en)
JP (1) JP2002500882A (en)
AU (1) AU762766B2 (en)
CA (1) CA2314246A1 (en)
WO (1) WO1999037755A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040019916A1 (en) * 1992-04-24 2004-01-29 Zarling David A. In vivo homologous sequence targeting in eukaryotic cells
WO1999037755A2 (en) * 1997-12-11 1999-07-29 Pangene Corporation The use of consensus sequences for targeted homologous gene isolation and recombination in gene families
AU3769000A (en) * 1999-03-22 2000-10-09 Pangene Corporation High-throughput gene cloning and phenotypic screening
US20020152494A1 (en) * 1999-09-14 2002-10-17 Sargent Roy Geoffrey Production of recombinant organisms
WO2001059683A2 (en) * 2000-02-11 2001-08-16 Pangene Corporation Integrated genomic services
CA2318517A1 (en) * 2000-09-15 2002-03-15 Her Majesty In Right Of Canada As Represented By The Minister Of Agricul Of Agriculture And Agri-Food Modulation of homologous recombination with single stranded dna binding proteins
WO2002027035A2 (en) * 2000-09-28 2002-04-04 Pangene Corporation High-throughput gene cloning and phenotypic screening
US20040063922A1 (en) * 2001-04-17 2004-04-01 Conrad Charles A. Methods and compositions for catalytic DNA exchange in a sequence specific manner
US20030082551A1 (en) * 2001-09-28 2003-05-01 Zarling David A. High-throughput gene cloning and phenotypic screening
GB0222229D0 (en) * 2002-09-25 2002-10-30 Univ Glasgow Mutant recombinases
ES2347239T3 (en) * 2002-12-02 2010-10-27 Amgen Fremont Inc. ANTIBODIES DIRECTED TO THE TUMOR NECROSIS FACTOR AND USES OF THE SAME.
WO2004060869A2 (en) * 2002-12-24 2004-07-22 Board Of Regents, The University Of Texas System Methods and compositions for catalytic dna exchange in a sequence manner
US20060275792A1 (en) * 2004-11-15 2006-12-07 Lee Jun E Enhancement of nucleic acid amplification using double-stranded DNA binding proteins

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993005178A1 (en) * 1990-05-07 1993-03-18 Daikin Industries, Ltd. Diagnostic applications of double d-loop formation
WO1993022443A1 (en) * 1992-04-24 1993-11-11 Sri International In vivo homologous sequence targeting in eukaryotic cells
WO1998042727A1 (en) * 1997-03-21 1998-10-01 Sri International Sequence alterations using homologous recombination

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5510473A (en) 1990-11-09 1996-04-23 The United States Of American As Represented By The Secretary Of Health And Human Services Cloning of the recA gene from thermus aquaticus YT-1
EP0556330B1 (en) 1990-11-09 2002-05-22 The Government of the United States of America, as represented by the Secretary, Department of Health and Human Services Method of targeting dna
FR2680709B1 (en) 1991-08-26 1995-07-21 Barsacq Gabriel INTERCEPTOR, FIXED ON THE END OF THE SUCTION HOSE BEFORE THE ENTRY OF DUST INTO THE NETWORK OF CENTRALIZED SUCTION SYSTEMS.
DE4130118C2 (en) 1991-09-11 1995-04-20 Jagenberg Ag Device for coating a web of material guided around a counter roller, in particular a paper or cardboard web
US5763240A (en) * 1992-04-24 1998-06-09 Sri International In vivo homologous sequence targeting in eukaryotic cells
US5731411A (en) 1993-07-12 1998-03-24 The United States Of America As Represented By The Department Of Health And Human Services Promotion of homologous DNA pairing by RecA-derived peptides
EP0842283A1 (en) 1995-07-21 1998-05-20 THE UNITED STATES GOVERNMENT as represented by THE DEPARTMENT OF HEALTH AND HUMAN SERVICES RecA-ASSISTED CLONING OF DNA
WO1999037755A2 (en) * 1997-12-11 1999-07-29 Pangene Corporation The use of consensus sequences for targeted homologous gene isolation and recombination in gene families

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993005178A1 (en) * 1990-05-07 1993-03-18 Daikin Industries, Ltd. Diagnostic applications of double d-loop formation
WO1993022443A1 (en) * 1992-04-24 1993-11-11 Sri International In vivo homologous sequence targeting in eukaryotic cells
WO1998042727A1 (en) * 1997-03-21 1998-10-01 Sri International Sequence alterations using homologous recombination

Also Published As

Publication number Publication date
AU1913599A (en) 1999-08-09
AU762766B2 (en) 2003-07-03
WO1999037755A2 (en) 1999-07-29
US20040091885A1 (en) 2004-05-13
US6524856B1 (en) 2003-02-25
CA2314246A1 (en) 1999-07-29
EP1038017A2 (en) 2000-09-27
JP2002500882A (en) 2002-01-15

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