WO1999036517A3 - Method for selecting ribozymes which are capable of covalently modifying the ribonucleic acids on 2'-oh-groups in trans - Google Patents

Method for selecting ribozymes which are capable of covalently modifying the ribonucleic acids on 2'-oh-groups in trans Download PDF

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Publication number
WO1999036517A3
WO1999036517A3 PCT/EP1999/000181 EP9900181W WO9936517A3 WO 1999036517 A3 WO1999036517 A3 WO 1999036517A3 EP 9900181 W EP9900181 W EP 9900181W WO 9936517 A3 WO9936517 A3 WO 9936517A3
Authority
WO
WIPO (PCT)
Prior art keywords
ribozymes
ribonucleic acids
trans
groups
covalently modifying
Prior art date
Application number
PCT/EP1999/000181
Other languages
German (de)
French (fr)
Other versions
WO1999036517A2 (en
Inventor
Andreas Jenne
Original Assignee
Andreas Jenne
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Andreas Jenne filed Critical Andreas Jenne
Priority to EP99907369A priority Critical patent/EP1060246A2/en
Publication of WO1999036517A2 publication Critical patent/WO1999036517A2/en
Publication of WO1999036517A3 publication Critical patent/WO1999036517A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus

Abstract

The invention relates to an in vitro selection method for selecting ribozymes which are capable of covalently modifying the ribonucleic acids on 2'-OH-groups in trans and to the ribozymes obtained using this method. The invention also relates to medicaments containing said ribozymes, said medicaments being preferably used for inhibiting gene expression - for example in gene therapy. The inventive ribozymes can also be used for producing muteins and nuclease-resistant ribonucleic acids, for example ribonuclease-resistant 'antisense' oligonucleotides.
PCT/EP1999/000181 1998-01-14 1999-01-14 Method for selecting ribozymes which are capable of covalently modifying the ribonucleic acids on 2'-oh-groups in trans WO1999036517A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP99907369A EP1060246A2 (en) 1998-01-14 1999-01-14 Method for selecting ribozymes which are capable of covalently modifying the ribonucleic acids on 2'-oh-groups in trans

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19801153.9 1998-01-14
DE1998101153 DE19801153A1 (en) 1998-01-14 1998-01-14 Selection of a ribozymes useful for, e.g. inhibiting gene expression and improving nuclease resistance

Publications (2)

Publication Number Publication Date
WO1999036517A2 WO1999036517A2 (en) 1999-07-22
WO1999036517A3 true WO1999036517A3 (en) 1999-09-23

Family

ID=7854593

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/000181 WO1999036517A2 (en) 1998-01-14 1999-01-14 Method for selecting ribozymes which are capable of covalently modifying the ribonucleic acids on 2'-oh-groups in trans

Country Status (3)

Country Link
EP (1) EP1060246A2 (en)
DE (1) DE19801153A1 (en)
WO (1) WO1999036517A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4589000A (en) * 1999-04-30 2000-11-17 Cyclops Genome Sciences Limited Polynucleotides
JP4745577B2 (en) 1999-11-24 2011-08-10 ザ リサーチ ファウンデイション オブ ステイト ユニバーシティー オブ ニューヨーク Catalytic RNAs with aminoacylation activity
ATE437946T1 (en) * 2002-02-15 2009-08-15 Univ New York State Res Found RIBOZYMES WITH BROAD TRNA AMINOACYLATION ACTION
US8188260B2 (en) 2005-12-06 2012-05-29 The University Of Tokyo Versatile acylation catalytic RNAs and uses thereof

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
HAGER, A. ET AL.: "Ribozymes : aiming at RNA replication and protein synthesis", CHEMISTRY AND BIOLOGY., vol. 3, September 1996 (1996-09-01), pages 717 - 725, XP002104957, ISSN: 1074-5521 *
IBBA M: "STRATEGIES FOR IN-VITRO AND IN-VIVO TRANSLATION WITH NON-NATURAL AMINO-ACIDS", BIOTECHNOLOGY & GENETIC ENGINEERING REVIEWS, (1996) VOL. 13, PP. 197-216., XP002109693 *
ILLANGASEKARE M ET AL: "AMINOACYL-RNA SYNTHESIS CATALYZED BY AN RNA", SCIENCE, vol. 267, 3 February 1995 (1995-02-03), pages 643 - 647, XP002044710, ISSN: 0036-8075 *
JENNE A ET AL: "A novel ribozyme with ester transferase activity", CHEMISTRY & BIOLOGY, VOL. 5, NO. 1, PP. 23-34., 15 January 1998 (1998-01-15), XP002109695 *
LORSCH J R ET AL: "IN VITRO EVOLUTION OF NEW RIBOZYMES WITH POLYNUCLEOTIDE KINASE ACTIVITY", NATURE, vol. 371, 1 September 1994 (1994-09-01), pages 31 - 36, XP002044711, ISSN: 0028-0836 *
PAN, T.: "Novel and variant ribozymes obtained through in vitro selection", CURRENT OPINION IN CHEMICAL BIOLOGY, vol. 1, 1997, pages 17 - 25, XP002109694 *

Also Published As

Publication number Publication date
EP1060246A2 (en) 2000-12-20
WO1999036517A2 (en) 1999-07-22
DE19801153A1 (en) 1999-07-15

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