WO1999033959A3 - Proteases from gram positive organisms - Google Patents

Proteases from gram positive organisms Download PDF

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Publication number
WO1999033959A3
WO1999033959A3 PCT/US1998/026972 US9826972W WO9933959A3 WO 1999033959 A3 WO1999033959 A3 WO 1999033959A3 US 9826972 W US9826972 W US 9826972W WO 9933959 A3 WO9933959 A3 WO 9933959A3
Authority
WO
WIPO (PCT)
Prior art keywords
present
metalloprotease
gram positive
provides
proteases
Prior art date
Application number
PCT/US1998/026972
Other languages
French (fr)
Other versions
WO1999033959A2 (en
Inventor
David A Estell
Original Assignee
Genencor Int
David A Estell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int, David A Estell filed Critical Genencor Int
Priority to AU19288/99A priority Critical patent/AU1928899A/en
Priority to EP98964092A priority patent/EP1042488A2/en
Publication of WO1999033959A2 publication Critical patent/WO1999033959A2/en
Publication of WO1999033959A3 publication Critical patent/WO1999033959A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)

Abstract

The present invention relates to the identification of novel metalloprotease in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.
PCT/US1998/026972 1997-12-30 1998-12-17 Proteases from gram positive organisms WO1999033959A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU19288/99A AU1928899A (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms
EP98964092A EP1042488A2 (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9727468.2A GB9727468D0 (en) 1997-12-30 1997-12-30 Proteases from gram positive organisms
GB9727468.2 1997-12-30

Publications (2)

Publication Number Publication Date
WO1999033959A2 WO1999033959A2 (en) 1999-07-08
WO1999033959A3 true WO1999033959A3 (en) 1999-11-25

Family

ID=10824311

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/026972 WO1999033959A2 (en) 1997-12-30 1998-12-17 Proteases from gram positive organisms

Country Status (4)

Country Link
EP (1) EP1042488A2 (en)
AU (1) AU1928899A (en)
GB (1) GB9727468D0 (en)
WO (1) WO1999033959A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9727470D0 (en) * 1997-12-30 1998-02-25 Genencor Int Bv Proteases from gram positive organisms

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0369817A2 (en) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Bacillus strains

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0369817A2 (en) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Bacillus strains

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
DATABASE BIOSIS BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; CALOGERO, SABINA ET AL: "RocR, A novel regulatory protein controlling arginine utilization in Bacillus subtilis, belongs to the NtrC/NifA family of transcriptional activators.", XP002105661 *
DATABASE EMBL NUCLEOTIDE SEQU 1 January 1900 (1900-01-01), XP002105644, Database accession no. Z99123 *
DATABASE EMBL NUCLEOTIDE SEQU 1 January 1900 (1900-01-01), XP002105645, Database accession no. S39732 *
DATABASE EMBL NUCLEOTIDE SEQU 1 January 1900 (1900-01-01), XP002105659, Database accession no. X73124 *
DATABASE EMBL NUCLEOTIDE SEQU 1 January 1900 (1900-01-01), XP002105660, Database accession no. P39635 *
GLASER P ET AL: "Bacillus subtilis genome project: cloning and sequencing of the 97kB region from 325-333", MOLECULAR MICROBIOLOGY, vol. 10, no. 2, 1993, pages 371 - 384, XP002105658 *
JOURNAL OF BACTERIOLOGY, (1994) VOL. 176, NO. 5, PP. 1234-1241. ISSN: 0021-9193. *
KUNST F ET AL: "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis", NATURE, vol. 390, 20 November 1997 (1997-11-20), pages 249 - 256, TABLE 1, XP002080813 *

Also Published As

Publication number Publication date
AU1928899A (en) 1999-07-19
EP1042488A2 (en) 2000-10-11
WO1999033959A2 (en) 1999-07-08
GB9727468D0 (en) 1998-02-25

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