WO1999001548A3 - Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin - Google Patents
Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin Download PDFInfo
- Publication number
- WO1999001548A3 WO1999001548A3 PCT/IB1998/001023 IB9801023W WO9901548A3 WO 1999001548 A3 WO1999001548 A3 WO 1999001548A3 IB 9801023 W IB9801023 W IB 9801023W WO 9901548 A3 WO9901548 A3 WO 9901548A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- insulin
- protein
- vector
- recombinant
- heterologous protein
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/73—Expression systems using phage (lambda) regulatory sequences
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Endocrinology (AREA)
- Diabetes (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Priority Applications (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IL13383298A IL133832A0 (en) | 1997-07-02 | 1998-07-02 | Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin |
PL98348669A PL194141B1 (en) | 1997-07-02 | 1998-07-02 | Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin |
EP98928476A EP0996713B1 (en) | 1997-07-02 | 1998-07-02 | Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin |
CA2294760A CA2294760C (en) | 1997-07-02 | 1998-07-02 | Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin |
DE69826864T DE69826864T2 (en) | 1997-07-02 | 1998-07-02 | VECTOR FOR HETEROLOGICAL PROTEIN EXPRESSION AND METHOD FOR EXTRACTION OF RECOMBINANT PROTEINS AND FOR INSULATION OF RECOMBINANT INSULINS |
AT98928476T ATE278776T1 (en) | 1997-07-02 | 1998-07-02 | VECTOR FOR HETEROLOGUE PROTEIN EXPRESSION AND METHOD FOR EXTRACTION OF RECOMBINANT PROTEINS AND FOR ISOLATION OF RECOMBINANT INSULINS |
BRPI9810650-3A BR9810650B1 (en) | 1997-07-02 | 1998-07-02 | vector for heterologous protein expression and methods for extracting recombinant protein and for purifying isolated recombinant insulin. |
IL133832A IL133832A (en) | 1997-07-02 | 1999-12-30 | Expression vector for transforming gram negative bacteria and process for producing a heterologous protein from the vector |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/886,967 US6068993A (en) | 1997-07-02 | 1997-07-02 | Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin |
US08/886,967 | 1997-07-02 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO1999001548A2 WO1999001548A2 (en) | 1999-01-14 |
WO1999001548A3 true WO1999001548A3 (en) | 1999-09-10 |
Family
ID=25390172
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IB1998/001023 WO1999001548A2 (en) | 1997-07-02 | 1998-07-02 | Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin |
Country Status (11)
Country | Link |
---|---|
US (4) | US6068993A (en) |
EP (1) | EP0996713B1 (en) |
AT (1) | ATE278776T1 (en) |
BR (1) | BR9810650B1 (en) |
CA (2) | CA2687542C (en) |
DE (1) | DE69826864T2 (en) |
IL (2) | IL133832A0 (en) |
IN (1) | IN187721B (en) |
PL (1) | PL194141B1 (en) |
RU (1) | RU2222598C2 (en) |
WO (1) | WO1999001548A2 (en) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7049286B2 (en) * | 2001-08-30 | 2006-05-23 | Diatos, S.A. | Insulin conjugates and methods of use thereof |
WO2004042003A2 (en) * | 2002-11-01 | 2004-05-21 | Promega Corporation | Cell lysis compositions, methods of use, apparatus, and kit |
EP1670446A2 (en) | 2003-09-26 | 2006-06-21 | Japan Tobacco Inc. | Method of inhibiting remnant lipoprotein production |
US7674621B2 (en) | 2004-05-21 | 2010-03-09 | The United States Of America As Represented By The Department Of Health And Human Services | Plasmids and phages for homologous recombination and methods of use |
CN105801686B (en) | 2004-07-19 | 2020-04-07 | 比奥孔有限公司 | Insulin-oligomer conjugates, formulations and uses thereof |
WO2006037215A1 (en) * | 2004-10-04 | 2006-04-13 | National Research Council Of Canada | Expression system, components thereof and methods of use |
US7935788B2 (en) * | 2004-10-04 | 2011-05-03 | National Research Council Of Canada | Reverse cumate repressor mutant |
WO2009032664A2 (en) * | 2007-08-31 | 2009-03-12 | The Regents Of The University Of Michigan | Methods and compositions for increasing biological molecule stability |
EP2203181B1 (en) | 2007-10-16 | 2018-02-14 | Biocon Limited | An orally administerable solid pharmaceutical composition and a process thereof |
ES2799431T3 (en) * | 2010-03-31 | 2020-12-17 | Amsilk Gmbh | Insoluble target protein separation |
RU2497118C1 (en) * | 2012-03-06 | 2013-10-27 | Федеральное государственное бюджетное учреждение науки Институт биохимии и физиологии микроорганизмов им. Г.К. Скрябина Российской академии наук (ИБФМ РАН) | FAST METHOD FOR TREATING INTESTINAL SMOOTH MUSCLE CELLS WITH Bacillus cereus HEMOLYSIN II |
CN102617703A (en) * | 2012-04-14 | 2012-08-01 | 拜明(苏州)生物技术有限公司 | Method for extracting exogenous expressive protein in fermentation bacteria efficiently without cracking |
WO2015176067A2 (en) * | 2014-05-16 | 2015-11-19 | The Regents Of The University Of California | A long non-coding rna expressed in aggressive cancer |
EP3344651B1 (en) | 2015-09-02 | 2022-03-02 | Merck Sharp & Dohme Corp. | A process for obtaining insulin with correctly formed disulfide bonds |
Citations (3)
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EP0264250A2 (en) * | 1986-10-14 | 1988-04-20 | Eli Lilly And Company | Process for transforming a human insulin precursor to human insulin |
EP0596459A2 (en) * | 1986-02-03 | 1994-05-11 | Cambridge Biotech Corporation | Process for purifying recombinant proteins, and use of products thereof |
US5460954A (en) * | 1992-04-01 | 1995-10-24 | Cheil Foods & Chemicals, Inc. | Production of human proinsulin using a novel vector system |
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US4624926A (en) * | 1981-01-02 | 1986-11-25 | The Research Foundation Of State University Of New York | Novel cloning vehicles for polypeptide expression in microbial hosts |
NZ199391A (en) * | 1981-01-02 | 1985-12-13 | Genentech Inc | Chimeric polypeptides comprising a proinsulin sequence,and preparation by recombinant dna technique;production of human insulin |
US4503155A (en) * | 1982-02-01 | 1985-03-05 | Eli Lilly And Company | Multifunctional, cloning vectors for use in Streptomyces, Bacillus, and E. coli |
US4732859A (en) * | 1982-09-03 | 1988-03-22 | Eli Lilly And Company | Method for conferring bacteriophage resistance to bacteria |
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US5026639A (en) * | 1988-01-14 | 1991-06-25 | Nippon Mining Company, Limited | Method to improve mRNA translation and use thereof for production of platelet factor-4 |
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-
1997
- 1997-07-02 US US08/886,967 patent/US6068993A/en not_active Expired - Lifetime
-
1998
- 1998-07-02 IN IN1479MA1998 patent/IN187721B/en unknown
- 1998-07-02 PL PL98348669A patent/PL194141B1/en unknown
- 1998-07-02 BR BRPI9810650-3A patent/BR9810650B1/en not_active IP Right Cessation
- 1998-07-02 RU RU2000102364/13A patent/RU2222598C2/en active
- 1998-07-02 DE DE69826864T patent/DE69826864T2/en not_active Expired - Lifetime
- 1998-07-02 AT AT98928476T patent/ATE278776T1/en active
- 1998-07-02 CA CA2687542A patent/CA2687542C/en not_active Expired - Lifetime
- 1998-07-02 IL IL13383298A patent/IL133832A0/en active IP Right Grant
- 1998-07-02 CA CA2294760A patent/CA2294760C/en not_active Expired - Lifetime
- 1998-07-02 EP EP98928476A patent/EP0996713B1/en not_active Expired - Lifetime
- 1998-07-02 WO PCT/IB1998/001023 patent/WO1999001548A2/en active IP Right Grant
-
1999
- 1999-05-07 US US09/306,949 patent/US6281329B1/en not_active Expired - Lifetime
- 1999-05-07 US US09/307,217 patent/US6509452B1/en not_active Expired - Lifetime
- 1999-12-30 IL IL133832A patent/IL133832A/en not_active IP Right Cessation
-
2000
- 2000-02-29 US US09/515,534 patent/US6699692B2/en not_active Expired - Lifetime
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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EP0596459A2 (en) * | 1986-02-03 | 1994-05-11 | Cambridge Biotech Corporation | Process for purifying recombinant proteins, and use of products thereof |
EP0264250A2 (en) * | 1986-10-14 | 1988-04-20 | Eli Lilly And Company | Process for transforming a human insulin precursor to human insulin |
US5460954A (en) * | 1992-04-01 | 1995-10-24 | Cheil Foods & Chemicals, Inc. | Production of human proinsulin using a novel vector system |
Non-Patent Citations (5)
Title |
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ETZERODT ET AL: "Iterative in vitro refolding of recombinant proteins", PROTEIN ENGINEERING (SUPPL), vol. 8, 1995, pages 89, XP002107890 * |
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MAKRIDES: "Strategies for Achieving High-Level Expression of Genes in Escherichia coli", MICROBIOLOGICAL REVIEWS, vol. 60, no. 3, September 1996 (1996-09-01), pages 512 - 538, XP002095235 * |
NILSSON ET AL: "Integrated production of human insulin and its C-peptide", JOURNAL OF BIOTECHNOLOGY, vol. 48, no. 3, 31 July 1996 (1996-07-31), pages 241-250, XP004037021 * |
WANG ET AL: "An efficient temperature-inducible vector incorporating the T7 gene 10 translation initiation leader region", NUCLEIC ACIDS RESEARCH, vol. 18, no. 4, 1990, pages 1070, XP002095234 * |
Also Published As
Publication number | Publication date |
---|---|
BR9810650A (en) | 2002-07-23 |
WO1999001548A2 (en) | 1999-01-14 |
EP0996713A1 (en) | 2000-05-03 |
DE69826864D1 (en) | 2004-11-11 |
US6699692B2 (en) | 2004-03-02 |
PL194141B1 (en) | 2007-04-30 |
CA2294760C (en) | 2010-02-23 |
CA2687542C (en) | 2013-04-30 |
PL348669A1 (en) | 2002-06-03 |
US6281329B1 (en) | 2001-08-28 |
DE69826864T2 (en) | 2005-11-03 |
US6068993A (en) | 2000-05-30 |
EP0996713B1 (en) | 2004-10-06 |
CA2687542A1 (en) | 1999-01-14 |
BR9810650B1 (en) | 2010-12-14 |
US6509452B1 (en) | 2003-01-21 |
IL133832A0 (en) | 2001-04-30 |
IL133832A (en) | 2007-07-04 |
US20030082742A1 (en) | 2003-05-01 |
IN187721B (en) | 2002-06-15 |
ATE278776T1 (en) | 2004-10-15 |
RU2222598C2 (en) | 2004-01-27 |
CA2294760A1 (en) | 1999-01-14 |
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