WO1999000516A3 - Neuronal stem cell gene - Google Patents

Neuronal stem cell gene Download PDF

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Publication number
WO1999000516A3
WO1999000516A3 PCT/GB1998/001862 GB9801862W WO9900516A3 WO 1999000516 A3 WO1999000516 A3 WO 1999000516A3 GB 9801862 W GB9801862 W GB 9801862W WO 9900516 A3 WO9900516 A3 WO 9900516A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
stem cell
cell gene
neuronal stem
sox1
Prior art date
Application number
PCT/GB1998/001862
Other languages
French (fr)
Other versions
WO1999000516A2 (en
Inventor
Robin Lovell-Badge
Laryssa H Pevny
Austin Smith
Original Assignee
Medical Res Council
Lovell Badge Robin
Laryssa H Pevny
Austin Smith
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Res Council, Lovell Badge Robin, Laryssa H Pevny, Austin Smith filed Critical Medical Res Council
Priority to AU82238/98A priority Critical patent/AU737409B2/en
Priority to EP98932288A priority patent/EP0990046A2/en
Priority to CA002295305A priority patent/CA2295305A1/en
Priority to JP50537999A priority patent/JP2002508666A/en
Publication of WO1999000516A2 publication Critical patent/WO1999000516A2/en
Publication of WO1999000516A3 publication Critical patent/WO1999000516A3/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Hospice & Palliative Care (AREA)
  • Neurology (AREA)
  • Oncology (AREA)
  • Neurosurgery (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention relates to a method for isolating a neuroblastic cell from a population of cells comprising the steps of: (a) detecting the expression of the Sox1 gene in the cells; and (b) sorting the cells to isolate those cells expressint the Sox1 gene, as well as to a method for causing a cell to become committed to the neural pathway comprising administering SOX1 thereto.
PCT/GB1998/001862 1997-06-25 1998-06-25 Neuronal stem cell gene WO1999000516A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU82238/98A AU737409B2 (en) 1997-06-25 1998-06-25 Neuronal stem cell gene
EP98932288A EP0990046A2 (en) 1997-06-25 1998-06-25 Neuronal stem cell gene
CA002295305A CA2295305A1 (en) 1997-06-25 1998-06-25 Neuronal stem cell gene
JP50537999A JP2002508666A (en) 1997-06-25 1998-06-25 Neural stem cell gene

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9713469.6A GB9713469D0 (en) 1997-06-25 1997-06-25 Neuronal stem cell gene
GB9713469.6 1997-06-25

Publications (2)

Publication Number Publication Date
WO1999000516A2 WO1999000516A2 (en) 1999-01-07
WO1999000516A3 true WO1999000516A3 (en) 1999-03-18

Family

ID=10814932

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1998/001862 WO1999000516A2 (en) 1997-06-25 1998-06-25 Neuronal stem cell gene

Country Status (6)

Country Link
EP (1) EP0990046A2 (en)
JP (1) JP2002508666A (en)
AU (1) AU737409B2 (en)
CA (1) CA2295305A1 (en)
GB (1) GB9713469D0 (en)
WO (1) WO1999000516A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9828383D0 (en) * 1998-12-22 1999-02-17 Medical Res Council Cell lineage markers
US7312081B2 (en) 2000-05-11 2007-12-25 The Trustees Of Columbia University In The City Of New York Genetic demonstration of requirement for nkx6.1, nkx2.2 and nkx6.2 in ventral neuron generation
US6955802B1 (en) 2000-05-11 2005-10-18 The Trustees Of Columbia University In The City Of New York Homeodomain protein code specifying progenitor cell identify and neuronal fate in the ventral neural tube
US7393686B1 (en) 2000-05-11 2008-07-01 Trustees Of Columbia University In The City Of New York Genetic demonstration of requirement for NKX6.1 and NKX2.2 in ventral neuron generation
EP1315794A4 (en) * 2000-09-01 2005-01-26 Univ Columbia Genetic demonstration of requirement for nkx6.1, nkx2.2 and nkx6.2 in ventral neuron generation

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997002049A1 (en) * 1995-07-06 1997-01-23 Emory University Neuronal progenitor cells and uses thereof
US5639618A (en) * 1994-05-13 1997-06-17 Plurion, Inc. Method of isolating a lineage specific stem cell in vitro

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639618A (en) * 1994-05-13 1997-06-17 Plurion, Inc. Method of isolating a lineage specific stem cell in vitro
WO1997002049A1 (en) * 1995-07-06 1997-01-23 Emory University Neuronal progenitor cells and uses thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE MEDLINE US NATIONAL LIBRARY OF MEDICINE (NLM), BETHESDA, MD, US; PEVNY L H ET AL: "A role for SOX1 in neural determination.", XP002087359 *
DEVELOPMENT, (1998 MAY) 125 (10) 1967-78, ENGLAND: United Kingdom *
PEVNY L H ET AL: "Sox genes find their feet.", CURRENT OPINION IN GENETICS AND DEVELOPMENT, (1997 JUN) 7 (3) 338-44, XP002087357 *
REX M ET AL: "cSox21 exhibits a complex and dynamic pattern of transcription during embryonic development of the chick central nervous system.", MECHANISMS OF DEVELOPMENT, (1997 AUG) 66 (1-2) 39-53, XP002087358 *

Also Published As

Publication number Publication date
GB9713469D0 (en) 1997-09-03
AU737409B2 (en) 2001-08-16
AU8223898A (en) 1999-01-19
CA2295305A1 (en) 1999-01-07
WO1999000516A2 (en) 1999-01-07
EP0990046A2 (en) 2000-04-05
JP2002508666A (en) 2002-03-19

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