WO1999000407A3 - Improved bacillus thuringiensis toxin - Google Patents

Improved bacillus thuringiensis toxin Download PDF

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Publication number
WO1999000407A3
WO1999000407A3 PCT/EP1998/004033 EP9804033W WO9900407A3 WO 1999000407 A3 WO1999000407 A3 WO 1999000407A3 EP 9804033 W EP9804033 W EP 9804033W WO 9900407 A3 WO9900407 A3 WO 9900407A3
Authority
WO
WIPO (PCT)
Prior art keywords
improved
proteins
toxicity
cry9c
amino acids
Prior art date
Application number
PCT/EP1998/004033
Other languages
French (fr)
Other versions
WO1999000407A2 (en
Inventor
Roeck Sandra De
Rie Jeroen Van
Original Assignee
Plant Genetic Systems Nv
Roeck Sandra De
Rie Jeroen Van
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Plant Genetic Systems Nv, Roeck Sandra De, Rie Jeroen Van filed Critical Plant Genetic Systems Nv
Priority to AU88044/98A priority Critical patent/AU741600B2/en
Priority to EP98939581A priority patent/EP0989998A1/en
Priority to CA002290718A priority patent/CA2290718A1/en
Publication of WO1999000407A2 publication Critical patent/WO1999000407A2/en
Publication of WO1999000407A3 publication Critical patent/WO1999000407A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Pest Control & Pesticides (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Insects & Arthropods (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

New improved Cry9C proteins, having significantly increased toxicity, and DNA sequences encoding these proteins, were designed. Analysis of amino acid positions in domain II of the Cry9C protein by protein mutagenesis identified amino acids involved in insect toxicity. Random replacement of these amino acids identifies proteins with improved toxicity. A combination of identified improved amino acids in a single protein yields modified Cry9C proteins with significantly improved toxicity.
PCT/EP1998/004033 1997-06-27 1998-06-25 Improved bacillus thuringiensis toxin WO1999000407A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU88044/98A AU741600B2 (en) 1997-06-27 1998-06-25 Improved bacillus thuringiensis toxin
EP98939581A EP0989998A1 (en) 1997-06-27 1998-06-25 Improved bacillus thuringiensis toxin
CA002290718A CA2290718A1 (en) 1997-06-27 1998-06-25 Improved bacillus thuringiensis toxin

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US88438997A 1997-06-27 1997-06-27
US08/884,389 1997-06-27

Publications (2)

Publication Number Publication Date
WO1999000407A2 WO1999000407A2 (en) 1999-01-07
WO1999000407A3 true WO1999000407A3 (en) 1999-05-14

Family

ID=25384517

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1998/004033 WO1999000407A2 (en) 1997-06-27 1998-06-25 Improved bacillus thuringiensis toxin

Country Status (4)

Country Link
EP (1) EP0989998A1 (en)
AU (1) AU741600B2 (en)
CA (1) CA2290718A1 (en)
WO (1) WO1999000407A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6570005B1 (en) 1996-07-01 2003-05-27 Mycogen Corporation Toxins active against pests
US6369213B1 (en) 1996-07-01 2002-04-09 Mycogen Corporation Toxins active against pests
CN1375009A (en) * 1999-09-17 2002-10-16 阿温提斯作物科学公司 Insect-resistant rice plants
ATE421973T1 (en) 1999-12-28 2009-02-15 Bayer Bioscience Nv INCECTICIDAL PROTEINS OF BACILLUS THURINGIENSIS
AU2001291259A1 (en) * 2000-08-11 2002-02-25 The Regents Of The University Of California Methods for blocking resistance to BT toxins in insects and nematodes
US7230167B2 (en) 2001-08-31 2007-06-12 Syngenta Participations Ag Modified Cry3A toxins and nucleic acid sequences coding therefor
EP1694846B1 (en) 2003-12-10 2014-10-01 Novozymes A/S A cell with improved secretion mediated by mrga protein or homologue
WO2005066202A2 (en) 2003-12-22 2005-07-21 E.I. Du Pont De Nemours And Company Bacillus cry9 family members
CN102260668B (en) 2004-03-25 2016-08-10 辛根塔参与股份公司 Corn event mir 604
JP4899180B2 (en) * 2004-12-22 2012-03-21 独立行政法人農業・食品産業技術総合研究機構 Primer set for nucleic acid test, test kit and test method using the same
US9522937B2 (en) 2007-03-28 2016-12-20 Syngenta Participations Ag Insecticidal proteins
UA96187C2 (en) 2007-03-28 2011-10-10 Сингента Партисипейшнс Аг Hybrid insecticidal proteins having activity against western corn rootworm or european corn borer
BRPI0924153A2 (en) * 2009-01-23 2016-05-24 Pioneer Hi Bred Int isolated nucleic acid molecule, DNA construct, host cell, transgenic plant, transformed seed, pesticide-isolated isolated polypeptide, composition and method for controlling an insect pest population, for exterminating an insect pest, for producing an active polypeptide pesticide and to protect a plant from a pest
DE102015113908B4 (en) 2015-08-21 2023-05-04 Truma Gerätetechnik GmbH & Co. KG level gauge

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0317511A2 (en) * 1987-11-18 1989-05-24 Ciba-Geigy Ag Insecticidal cotton plant cells
WO1990006999A1 (en) * 1988-12-12 1990-06-28 E.I. Du Pont De Nemours And Company New strain of bacillus thuringiensis
WO1994024264A1 (en) * 1993-04-09 1994-10-27 Plant Genetic Systems N.V. New bacillus thuringiensis strains and their insecticidal proteins

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0317511A2 (en) * 1987-11-18 1989-05-24 Ciba-Geigy Ag Insecticidal cotton plant cells
WO1990006999A1 (en) * 1988-12-12 1990-06-28 E.I. Du Pont De Nemours And Company New strain of bacillus thuringiensis
WO1994024264A1 (en) * 1993-04-09 1994-10-27 Plant Genetic Systems N.V. New bacillus thuringiensis strains and their insecticidal proteins

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DEAN D H ET AL: "Probing the mechanism of action of Bacillus thuringiensis insecticidal proteins by site-directed mutagenesis -- a minireview", GENE, vol. 179, no. 1, 7 November 1996 (1996-11-07), pages 111-117, XP004071972 *
HOFTE H ET AL: "INSECTICIDAL CRYSTAL PROTEINS OF BACILLUS THURINGIENSIS", MICROBIOLOGICAL REVIEWS, vol. 53, 1 June 1989 (1989-06-01), pages 242 - 255, XP000374163 *
KOZIEL M G ET AL: "FIELD PERFORMANCE OF ELITE TRANSGENIC MAIZE PLANTS EXPRESSING AN INSECTICIDAL PROTEIN DERIVED FROM BACILLUS THURINGIENSIS", BIO/TECHNOLOGY, vol. 11, February 1993 (1993-02-01), pages 194 - 200, XP002029715 *
LAMBERT B ET AL: "A BACILLUS THURINGIENSIS INSECTICIDAL CRYSTAL PROTEIN WITH A HIGH ACTIVITY AGAINST MEMBERS OF THE FAMILY NOCTUIDAE", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 62, no. 1, January 1996 (1996-01-01), pages 80 - 86, XP002045611 *
P. GROCHULSKI ET AL.,: "Bacillus thurigiensis CrylA(a) insecticidal toxin: Crystal structure and channel formation", JOURNAL OF MOLECULAR BIOLOGY, vol. 254, no. 3, 1995, pages 447 - 464, XP002095820 *
PEFEROEN M: "Progress and prospects for field use of Bt genes in crops", TRENDS IN BIOTECHNOLOGY, vol. 15, no. 5, May 1997 (1997-05-01), pages 173-177, XP004059846 *

Also Published As

Publication number Publication date
CA2290718A1 (en) 1999-01-07
AU741600B2 (en) 2001-12-06
EP0989998A1 (en) 2000-04-05
AU8804498A (en) 1999-01-19
WO1999000407A2 (en) 1999-01-07

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