WO1998055601A3 - Plant amino acid biosynthetic enzymes - Google Patents

Plant amino acid biosynthetic enzymes Download PDF

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Publication number
WO1998055601A3
WO1998055601A3 PCT/US1998/011692 US9811692W WO9855601A3 WO 1998055601 A3 WO1998055601 A3 WO 1998055601A3 US 9811692 W US9811692 W US 9811692W WO 9855601 A3 WO9855601 A3 WO 9855601A3
Authority
WO
WIPO (PCT)
Prior art keywords
enzyme
threonine
amino acid
acid biosynthetic
biosynthetic enzymes
Prior art date
Application number
PCT/US1998/011692
Other languages
French (fr)
Other versions
WO1998055601A2 (en
Inventor
Saverio Carl Falco
Stephen M Allen
J Antoni Rafalski
William D Hitz
Anthony John Kinney
Lynn Marie Abell
Catherine Jane Thorpe
Original Assignee
Du Pont
Saverio Carl Falco
Stephen M Allen
J Antoni Rafalski
William D Hitz
Anthony John Kinney
Lynn Marie Abell
Catherine Jane Thorpe
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Saverio Carl Falco, Stephen M Allen, J Antoni Rafalski, William D Hitz, Anthony John Kinney, Lynn Marie Abell, Catherine Jane Thorpe filed Critical Du Pont
Priority to EP98925282A priority Critical patent/EP0979296A2/en
Priority to BR9809967-1A priority patent/BR9809967A/en
Priority to AU77270/98A priority patent/AU7727098A/en
Priority to US09/424,978 priority patent/US6664445B1/en
Priority to CA002292778A priority patent/CA2292778A1/en
Publication of WO1998055601A2 publication Critical patent/WO1998055601A2/en
Publication of WO1998055601A3 publication Critical patent/WO1998055601A3/en
Priority to US10/734,698 priority patent/US7022895B2/en
Priority to US10/917,602 priority patent/US7368633B2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8253—Methionine or cysteine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)

Abstract

This invention relates to an isolated nucleic acid fragment encoding a plant enzyme that catalyzes steps in the biosynthesis of lysine, threonine, methionine, cysteine and isoleucine from aspartate, the enzyme a member selected from the group consisting of: dihydrodipicolinate reductase, diaminopimelate epimerase, threonine synthase, threonine deaminase and S-adenosylmethionine synthetase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the enzyme in a transformed host cell.
PCT/US1998/011692 1997-06-06 1998-06-05 Plant amino acid biosynthetic enzymes WO1998055601A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
EP98925282A EP0979296A2 (en) 1997-06-06 1998-06-05 Plant amino acid biosynthetic enzymes
BR9809967-1A BR9809967A (en) 1997-06-06 1998-06-05 Isolated nucleic acid fragment, chimeric gene, transformed host cell, polypeptide, method of altering the level of expression of an enzyme, method of obtaining a nucleic acid fragment, product and method for assessing the ability of at least one compound to inhibit the activity of an enzyme
AU77270/98A AU7727098A (en) 1997-06-06 1998-06-05 Plant amino acid biosynthetic enzymes
US09/424,978 US6664445B1 (en) 1997-06-06 1998-06-05 Plant amino acid biosynthetic enzymes
CA002292778A CA2292778A1 (en) 1997-06-06 1998-06-05 Plant amino acid biosynthetic enzymes
US10/734,698 US7022895B2 (en) 1997-06-06 2003-12-12 Plant amino acid biosynthetic enzymes
US10/917,602 US7368633B2 (en) 1997-06-06 2004-08-13 Plant amino acid biosynthetic enzymes

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US4877197P 1997-06-06 1997-06-06
US60/048,771 1997-06-06
US4944397P 1997-06-12 1997-06-12
US60/049,443 1997-06-12

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US09424978 A-371-Of-International 1998-06-05
US09/424,978 A-371-Of-International US6664445B1 (en) 1997-06-06 1998-06-05 Plant amino acid biosynthetic enzymes
US10/734,698 Division US7022895B2 (en) 1997-06-06 2003-12-12 Plant amino acid biosynthetic enzymes

Publications (2)

Publication Number Publication Date
WO1998055601A2 WO1998055601A2 (en) 1998-12-10
WO1998055601A3 true WO1998055601A3 (en) 1999-03-04

Family

ID=26726514

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/011692 WO1998055601A2 (en) 1997-06-06 1998-06-05 Plant amino acid biosynthetic enzymes

Country Status (8)

Country Link
US (2) US6664445B1 (en)
EP (2) EP1959016A3 (en)
CN (1) CN1259170A (en)
AR (1) AR014351A1 (en)
BR (1) BR9809967A (en)
CA (1) CA2292778A1 (en)
ID (1) ID24686A (en)
WO (1) WO1998055601A2 (en)

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BR9809967A (en) 2000-08-01
AR014351A1 (en) 2001-02-28
EP1959016A2 (en) 2008-08-20
CN1259170A (en) 2000-07-05
WO1998055601A2 (en) 1998-12-10

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