WO1998054310A3 - Methods for activating inactive genes and methods for altering the rate of development of cells, tissues and organisms - Google Patents

Methods for activating inactive genes and methods for altering the rate of development of cells, tissues and organisms Download PDF

Info

Publication number
WO1998054310A3
WO1998054310A3 PCT/US1998/010006 US9810006W WO9854310A3 WO 1998054310 A3 WO1998054310 A3 WO 1998054310A3 US 9810006 W US9810006 W US 9810006W WO 9854310 A3 WO9854310 A3 WO 9854310A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
cell
organism
rate
tissue
Prior art date
Application number
PCT/US1998/010006
Other languages
French (fr)
Other versions
WO1998054310A2 (en
Inventor
Yuying Tan
Original Assignee
Anticancer Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anticancer Inc filed Critical Anticancer Inc
Priority to EP98923455A priority Critical patent/EP1003848A2/en
Publication of WO1998054310A2 publication Critical patent/WO1998054310A2/en
Publication of WO1998054310A3 publication Critical patent/WO1998054310A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Public Health (AREA)
  • Biophysics (AREA)
  • Epidemiology (AREA)
  • Plant Pathology (AREA)
  • Veterinary Medicine (AREA)
  • Microbiology (AREA)
  • Animal Husbandry (AREA)
  • Biochemistry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention is based on the unexpected observation that DNA methylation, particularly at cytosine residues, that regulates gene activation states and the rate of development and differentiation of cells and organisms, can be altered by providing methioninase to a cell, tissue or organism. In addition, other methylation reactions within a cell can be altered by providing methioninase to a cell, tissue or organism. Based on these observations, the present invention provides methods of activating inactive genes, methods for decreasing the rate of development of cell, tissues or an organism, methods of treating obesity and methods of treating Parkinson's disease and other neurological disorders, by either providing a methioninase containing composition to a cell, tissue or organism or by introducing a methioninase encoding DNA molecule into the cell, tissue or organism. The present invention further provides organisms and cells that have been altered such that their rate of maturation is decreased and/or the degree of methylation of cellular constituents (DNA, RNA, lipids, proteins small cellular molecules, etc.) is decreased.
PCT/US1998/010006 1997-05-30 1998-05-15 Methods for activating inactive genes and methods for altering the rate of development of cells, tissues and organisms WO1998054310A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP98923455A EP1003848A2 (en) 1997-05-30 1998-05-15 Methods for activating inactive genes and methods for altering the rate of development of cells, tissues and organisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4803397P 1997-05-30 1997-05-30
US60/048,033 1997-05-30

Publications (2)

Publication Number Publication Date
WO1998054310A2 WO1998054310A2 (en) 1998-12-03
WO1998054310A3 true WO1998054310A3 (en) 1999-03-04

Family

ID=21952396

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/010006 WO1998054310A2 (en) 1997-05-30 1998-05-15 Methods for activating inactive genes and methods for altering the rate of development of cells, tissues and organisms

Country Status (2)

Country Link
EP (1) EP1003848A2 (en)
WO (1) WO1998054310A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7138384B1 (en) 1997-08-29 2006-11-21 The Regents Of The University Of California Modulators of DNA cytosine-5 methyltransferase and methods for use thereof
DE10038733A1 (en) * 2000-08-02 2002-02-21 Epigenomics Ag Procedure for determining the age of individuals

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996040284A1 (en) * 1995-06-07 1996-12-19 Anticancer, Inc. Use of methioninase in anti-methionine and anti-homocysteine chemotherapy

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996040284A1 (en) * 1995-06-07 1996-12-19 Anticancer, Inc. Use of methioninase in anti-methionine and anti-homocysteine chemotherapy

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
CHIU C.P., BLAU H.M.: "5-azacytidine permits gene activation in a previously noninducible cell type", CELL, vol. 40, no. 2, February 1985 (1985-02-01), US, pages 417 - 424, XP002082384 *
HOFFMAN R.M. AND TAN Y.: "Anti-methionine therapy as a potential universal modulator for anti-tumor and anti-metastatic therapy", CLINICAL AND EXPERIMENTAL METASTASIS, vol. 14,supp.1, September 1996 (1996-09-01), London, GB, pages 85, XP002082383 *
HOFFMAN R.M.: "Methioninase: a therapeutic for diseases related to altered methionine methabolism and transmethylation: cancer, heart disease, obesity aging and Parkinson's disease", HUMAN CELL, vol. 10, no. 1, March 1997 (1997-03-01), JP, pages 69 - 80, XP002082379 *
MAKOS M. ET AL.: "Distinct hypermethylation patterns occur at altered chromosome loci in human lung and colon cancer", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 89, March 1992 (1992-03-01), WASHINGTON US, pages 1929 - 1933, XP002082385 *
TAN Y. ET AL.: "Overexpression and large-scale production of recombinant l-methionine-alpha-deamino-gamma-mercaptomethane-lyase for novel anticancer therapy", PROTEIN EXPRESSION AND PURIFICATION, vol. 9, no. 2, March 1997 (1997-03-01), us, pages 233 - 245, XP002082381 *
TAN Y. ET AL.: "Serum Methionine depletion without side effects by methioninase in metastatic breast cancer patients", ANTICANCER RESEARCH, vol. 16, no. 6c, November 1996 (1996-11-01), GR, pages 3937 - 3942, XP002082380 *
TURKER M.S. ET AL.: "The mouse APRT gene as a model for studying epigenetic gene inactivation", ADVANCEDS IN EXPERIMENTAL MEDICINE AND BIOLOGY, vol. 370, 1995, US, pages 647 - 652, XP002082382 *

Also Published As

Publication number Publication date
WO1998054310A2 (en) 1998-12-03
EP1003848A2 (en) 2000-05-31

Similar Documents

Publication Publication Date Title
DE69533697T2 (en) ANTISENSE OLIGONUCLEOTIDE MODULATION OF Raf GENE EXPRESSION
Agrawal et al. Mixed-backbone oligonucleotides as second generation antisense oligonucleotides: in vitro and in vivo studies
CA2240494A1 (en) Therapeutic molecules generated by trans-splicing
DE69729179T2 (en) Patchy sugar-modified oligonucleotides
Altmann et al. Second generation of antisense oligonucleotides: from nuclease resistance to biological efficacy in animals
EP2123759B1 (en) Stabilized RNAS as transfection controls and silencing reagents
DE69934227T2 (en) Antisense oligonucleotides based on beta-arabinose and its analogues
DE69931029T2 (en) OPTIMIZED ANTISENSE OLIGONUCLEOTIDES COMPLEMENTARY TO DNA METHYLTRANSFERASE SEQUENCES
WO1999064576A3 (en) Human genes differentially expressed in colon cancer
DE69718600T2 (en) Polyether nucleic acids
CN102643818A (en) Oligoribonucleotides and methods of use thereof for treatment of alopecia, acute renal failure and other diseases
WO2003087367A3 (en) Means and methods for the specific inhibition of genes in cells and tissue of the cns and/or eye
Prakash et al. 2 ‘-O-[2-(Guanidinium) ethyl]-modified oligonucleotides: stabilizing effect on duplex and triplex structures
EP0519463A1 (en) Synthetic, catalytic oligonucleotides
CA2073500A1 (en) Compositions and methods for detecting and modulating rna activity and gene expression
WO1997045541A3 (en) Patched genes and their uses
WO1998019167A3 (en) Cell stress regulated human mhc class i gene
EP1100894A2 (en) Short oligonucleotides for the inhibition of vegf expression
DE19935303A1 (en) Oligonucleotides to inhibit the expression of human eg5
CA2083377A1 (en) Compositions and methods for modulating rna activity through modification of the 5' cap structure of rna
Prakash et al. 2 ‘-O-[2-(Amino)-2-oxoethyl] Oligonucleotides
CA2451603A1 (en) Process for facilitating nucleic acid transfer
WO2005119640A3 (en) Interfering stem-loop sequences and method for identifying
EP1417307B1 (en) Oligonucleotide compositions and their use to induce apoptosis
WO1998054310A3 (en) Methods for activating inactive genes and methods for altering the rate of development of cells, tissues and organisms

Legal Events

Date Code Title Description
AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 1998923455

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1998923455

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1998923455

Country of ref document: EP