WO1998054217A1 - Angiostatin fragments and method of use - Google Patents
Angiostatin fragments and method of use Download PDFInfo
- Publication number
- WO1998054217A1 WO1998054217A1 PCT/US1998/010979 US9810979W WO9854217A1 WO 1998054217 A1 WO1998054217 A1 WO 1998054217A1 US 9810979 W US9810979 W US 9810979W WO 9854217 A1 WO9854217 A1 WO 9854217A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- angiostatin
- plasminogen
- kringle
- cells
- tumor
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6435—Plasmin (3.4.21.7), i.e. fibrinolysin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21007—Plasmin (3.4.21.7), i.e. fibrinolysin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/827—Proteins from mammals or birds
- Y10S530/828—Cancer
Definitions
- the present invention relates to endothelial inhibitors, called angiostatin, which reversibly inhibit proliferation of endothelial cells. More particularly, the present invention relates to angiostatin proteins that can be isolated from body fluids such as blood or urine, or can be synthesized by recombinant, enzymatic or chemical methods. The angiostatin is capable of inhibiting angiogenesis related diseases and modulating angiogenic processes.
- the present invention relates to diagnostic assays and kits for angiostatin measurement, to histochemical kits for localization of angiostatin, to DNA sequences coding for angiostatin and molecular probes to monitor angiostatin biosynthesis, to antibodies that are specific for the angiostatin, to the development of protein agonists and antagonists to the angiostatin receptor, to anti-angiostatin receptor-specific antibody agonists and antagonists, and to cytotoxic agents linked to angiostatin proteins.
- angiogenesis means the generation of new blood vessels into a tissue or organ. Under normal physiological conditions, humans or animals undergo angiogenesis only in very specific restricted situations. For example, angiogenesis is normally observed in wound healing, fetal and embryonal development and formation of the corpus luteum, endometrium and placenta.
- endothelium means a thin layer of flat epithelial cells that lines serous cavities, lymph vessels, and blood vessels.
- Endothelial cells and pericytes surrounded by a basement membrane, form capillary blood vessels.
- Angiogenesis begins with the erosion of the basement membrane by enzymes released by endothelial cells and leukocytes.
- the endothelial cells which line the lumen of blood vessels, then protrude through the basement membrane.
- Angiogenic stimulants induce the endothelial cells to migrate through the eroded basement membrane.
- the migrating cells form a "sprout" off the parent blood vessel, where the endothelial cells undergo mitosis and proliferate.
- the endothelial sprouts merge with each other to form capillary loops, creating the new blood vessel.
- Persistent, unregulated angiogenesis occurs in a multiplicity of disease states, tumor metastasis and abnormal growth by endothelial cells and supports the pathological damage seen in these conditions.
- the diverse pathological disease states in which unregulated angiogenesis is present have been grouped together as angiogenic dependent or angiogenic associated diseases.
- Tumor 'take' has occurred, every increase in tumor cell population must be preceded by an increase in new capillaries converging on the tumor.”
- Tumor 'take' is currently understood to indicate a prevascular phase of tumor growth in which a population of tumor cells occupying a few cubic millimeters volume and not exceeding a few million cells, can survive on existing host microvessels. Expansion of tumor volume beyond this phase requires the induction of new capillary blood vessels. For example, pulmonary micrometastases in the early prevascular phase in mice would be undetectable except by high power microscopy on histological sections.
- Tumor growth in the avascular cornea proceeds slowly and at a linear rate, but switches to exponential growth after neovascularization.
- Tumor growth and neovascularization An experimental model using the rabbit cornea. J. Natl. Cancer Institute 52:41-427, 1974
- Tumors suspended in the aqueous fluid of the anterior chamber of the rabbit eye remain viable, avascular and limited in size to ⁇ 1 mm 3 . Once they are implanted on the iris vascular bed, they become neovascularized and grow rapidly, reaching 16,000 times their original volume within 2 weeks. (Gimbrone MA Jr., et al, Tumor dormancy in vivo by prevention of neovascularization. J. Exp. Med. 136:261 - 276)
- Vascular casts of metastases in the rabbit liver reveal heterogeneity in size of the metastases, but show a relatively uniform cut-off point for the size at which vascularization is present.
- Tumors are generally avascular up to 1 mm in diameter, but are neovascularized beyond that diameter.
- pre-vascular hyperplastic islets are limited in size to ⁇ 1 mm.
- 4- 10% of the islets become neovascularized, and from these islets arise large vascularized tumors of more than 1000 times the volume of the pre-vascular islets.
- VEGF vascular endothelial growth factor
- Anti-bFGF monoclonal antibody causes 70% inhibition of growth of a mouse tumor which is dependent upon secretion of bFGF as its only mediator of angiogenesis.
- the antibody does not inhibit growth of the tumor cells in vitro. (Hori A, et al, Suppression of solid tumor growth by immunoneutralizing monoclonal antibody against human basic fibroblast growth factor. Cancer Research, 51 :6180-6184, 1991)
- bFGF Intraperitoneal injection of bFGF enhances growth of a primary tumor and its metastases by stimulating growth of capillary endothelial cells in the tumor.
- the tumor cells themselves lack receptors for bFGF, and bFGF is not a mitogen for the tumors cells in vitro.
- a specific angiogenesis inhibitor (AGM-1470) inhibits tumor growth and metastases in vivo, but is much less active in inhibiting tumor cell proliferation in vitro. It inhibits vascular endothelial cell proliferation half-maximally at 4 logs lower concentration than it inhibits tumor cell proliferation.
- Angioinhibins Synthetic analogues of fumagillin which inhibit angiogenesis and suppress tumor growth. Nature, 48:555-557, 1990.
- tumor growth is angiogenesis dependent.
- Carcinoma of the ovary metastasizes to the peritoneal membrane as tiny avascular white seeds (1-3 mm 3 ). These implants rarely grow larger until one or more of them becomes neovascularized.
- Tumor angiogenesis A new significant and independent prognostic indicator in early- stage breast carcinoma, J Natl. Cancer Inst. 84: 1875- 1887, 1992) and in prostate cancer (Weidner N, Carroll PR, Flax J, Blumenfeld W, Folkman J. Tumor angiogenesis correlates with metastasis in invasive prostate carcinoma. American Journal of Pathology, 143(2):401-409, 1993) correlates highly with risk of future metastasis.
- angiogenesis plays a major role in the metastasis of a cancer. If this angiogenic activity could be repressed or eliminated, then the tumor, although present, would not grow. In the disease state, prevention of angiogenesis could avert the damage caused by the invasion of the new micro vascular system. Therapies directed at control of the angiogenic processes could lead to the abrogation or mitigation of these diseases. What is needed therefore is a composition and method which can inhibit the unwanted growth of blood vessels, especially into tumors. Also needed is a method for detecting, measuring, and localizing the composition.
- the composition should be able to overcome the activity of endogenous growth factors in premetastatic tumors and prevent the formation of the capillaries in the tumors thereby inhibiting the growth of the tumors.
- the composition, fragments of the composition, and antibodies specific to the composition should also be able to modulate the formation of capillaries in other angiogenic processes, such as wound healing and reproduction.
- the composition and method for inhibiting angiogenesis should preferably be non-toxic and produce few side effects. Also needed is a method for detecting, measuring, and localizing the binding sites for the composition as well as sites of biosynthesis of the composition.
- the composition and fragments of the composition should be capable of being conjugated to other molecules for both radioactive and non- radioactive labeling purposes
- compositions and methods are provided that are effective for modulating angiogenesis, and inhibiting unwanted angiogenesis, especially angiogenesis related to tumor growth.
- the present invention includes a protein, which has been named "angiostatin", defined by its ability to overcome the angiogenic activity of endogenous growth factors such as bFGF, in vitro, and by it amino acid sequence homology and structural similarity to an internal portion of plasminogen beginning at approximately plasminogen amino acid 98.
- Angiostatin comprises a protein having a molecular weight of between approximately 38 kilodaltons and 45 kilodaltons as determined by reducing polyacrylamide gel electrophoresis and having an amino acid sequence substantially similar to that of a fragment of murine plasminogen beginning at amino acid number 98 of an intact murine plasminogen molecule (SEQ ID NO:2).
- amino acid sequence of angiostatin varies slightly between species.
- human angiostatin the amino acid sequence is substantially similar to the sequence of the above described murine plasminogen fragment, although an active human angiostatin sequence may start at either amino acid number 97 or 99 of an intact human plasminogen amino acid sequence.
- fragments of human plasminogen has similar anti-angiogenic activity as shown in a mouse tumor model. It is to be understood that the number of amino acids in the active angiostatin molecule may vary and all amino acid sequences that have endothelial inhibiting activity are contemplated as being included in the present invention.
- the present invention provides methods and compositions for treating diseases and processes mediated by undesired and uncontrolled angiogenesis by administering to a human or animal a composition comprising a substantially purified angiostatin or angiostatin derivative in a dosage sufficient to inhibit angiogenesis.
- the present invention is particularly useful for treating, or for repressing the growth of, tumors. Administration of angiostatin to a human or animal with prevascularized metastasized tumors will prevent the growth or expansion of those tumors.
- the present invention also encompasses DNA sequences encoding angiostatin, expression vectors containing DNA sequences encoding angiostatin, and cells containing one or more expression vectors containing DNA sequences encoding angiostatin.
- the present invention further encompasses gene therapy methods whereby DNA sequences encoding angiostatin are introduced into a patient to modify in vivo angiostatin levels.
- the present invention also includes diagnostic methods and kits for detection and measurement of angiostatin in biological fluids and tissues, and for localization of angiostatin in tissues and cells.
- the diagnostic method and kit can be in any configuration well known to those of ordinary skill in the art.
- the present invention also includes antibodies specific for the angiostatin molecule and portions thereof, and antibodies that inhibit the binding of antibodies specific for the angiostatin. These antibodies can be polyclonal antibodies or monoclonal antibodies.
- the antibodies specific for the angiostatin can be used in diagnostic kits to detect the presence and quantity of angiostatin which is diagnostic or prognostic for the occurrence or recurrence of cancer or other disease mediated by angiogenesis.
- Antibodies specific for angiostatin may also be administered to a human or animal to passively immunize the human or animal against angiostatin, thereby reducing angiogenic inhibition.
- the present invention also includes diagnostic methods and kits for detecting the presence and quantity of antibodies that bind angiostatin in body fluids.
- the diagnostic method and kit can be in any configuration well known to those of ordinary skill in the art.
- the present invention also includes anti-angiostatin receptor-specific antibodies that bind to the angiostatin receptor and transmit the appropriate signal to the cell and act as agonists or antagonists.
- the present invention also includes angiostatin protein fragments and analogs that can be labeled isotopically or with other molecules or proteins for use in the detection and visualization of angiostatin binding sites with techniques, including, but not limited to, positron emission tomography, autoradiography, flow cytometry, radioreceptor binding assays, and immunohistochemistry.
- angiostatin proteins and analogs also act as agonists and antagonists at the angiostatin receptor, thereby enhancing or blocking the biological activity of angiostatin. Such proteins are used in the isolation of the angiostatin receptor.
- the present invention also includes angiostatin, angiostatin fragments, angiostatin antisera, or angiostatin receptor agonists and angiostatin receptor antagonists linked to cytotoxic agents for therapeutic and research applications. Still further, angiostatin, angiostatin fragments, angiostatin antisera, angiostatin receptor agonists and angiostatin receptor antagonists are combined with pharmaceutically acceptable excipients, and optionally sustained-release compounds or compositions, such as biodegradable polymers, to form therapeutic compositions.
- the present invention includes molecular probes for the ribonucleic acid and deoxyribonucleic acid involved in transcription and translation of angiostatin. These molecular probes provide means to detect and measure angiostatin biosynthesis in tissues and cells.
- an object of the present invention to provide a composition comprising an angiostatin.
- Still another object of the present invention is to provide a composition consisting of antibodies to angiostatin that are selective for specific regions of the angiostatin molecule that do not recognize plasminogen. It is another object of the present invention to provide a method for the detection or prognosis of cancer.
- Yet another object of the invention is to provide compositions and methods useful for gene therapy for the modulation of angiogenic processes.
- Figure 1 shows SEQ ID NO: l, the amino acid sequence of the whole murine plasminogen.
- Figure 2 shows the beginning sequence of the angiostatin for murine (SEQ ID NO:2) and compares the murine sequence with corresponding human (SEQ ID NO: 3), Rhesus monkey (SEQ ID NO:4), porcine (SEQ ID NO:5) and bovine (SEQ ID NO: 6) plasminogen protein fragments. The mouse sequence is listed first, followed by human, Rhesus, porcine and bovine.
- Figure 3 shows BrdU labeling index of tumor cells in the lung in the presence or absence of a primary tumor.
- Figure 4 shows Matrigel analysis of the influence of a Lewis lung primary tumor on bFGF driven angiogenesis in vivo.
- Figure 5 shows dose response curve for serum derived from mice bearing Lewis lung carcinoma (LLC-Low) versus serum from normal mice.
- Bovine capillary endothelial cells were assayed in a bFGF-driven 72-hour proliferation assay.
- Figure 6 shows that both low and high metastatic tumors contain endothelial mitogenic activity in their ascites, but only the low metastatic tumor line has endothelial inhibitory activity in the serum.
- Figure 7 shows a C4 Reverse Phase Chromatographic profile of partially purified serum or urine from tumor- bearing animals.
- Figure 8 shows surface lung metastases after the 13 day treatment of mice with intact plasminogen molecule, active fraction from a lysine binding site I preparation of human plasminogen, concentrated urine from tumor bearing mice and concentrated urine from normal mice.
- Figure 9 shows lung weight after the 13 day treatment of mice with intact plasminogen molecule of human plasminogen, active fraction from lysine binding site I preparation, concentrated urine from tumor bearing mice and concentrated urine from normal mice.
- Figure 10 is a schematic representation of the pTrcHis vector.
- Figure 11 depicts an immunoblot of E.coli expressed human angiostatin from a 10L scaled-up fermentation, probed with monoclonal antibody against human plasminogen kringle region 1-3. Arrow shows recombinant human angiostatin. A) shows recombinant angiostatin eluted with 0.2 M amino caproic acid; B) shows the last wash with 1 X PBS of the lysine column; and C) shows clarified lysate from cracked cells.
- Figure 12 Is a graph depicting percent inhibition of growing bovine capillary endothelial cells as a function of dilution of stock; Al, A2, B l , B2, and E are recombinant clones that express human angiostatin anit-angiogenesis activity; CI, C2, Dl and D2 controls are negative controls clones containing vector only without the human DNA sequence coding for angiostatin.
- Figure 13 shows the inhibitory effect on proliferation of recombinant human angiostatin on bovine capillary endothelial cells in vitro.
- Figure 14 shows the growth proliferation index and apoptotic index after removal of the primary tumor and treatment with saline or a fumagillin analogue with anti- angiogenic activity
- Figure 15 shows the inhibition of growth of a T241 primary tumor in mice by treatment with human angiostatin in vivo with a single injection of 40 mg/kg/day.
- Figure 16 shows the inhibition of growth of a LLC-LM primary tumor in mice by treatment with human angiostatin in vivo at two doses of 40 mg/kg per dose (80 mg/kg/day).
- Figure 17 shows the effect of the removal of a Lewis lung carcinoma primary tumor on the growth of its lung metastases.
- Figure 18 shows the growth proliferation and apoptotic index after tumor resection
- Figure 19 shows the effect of administration of angiostatin protein to mice having implated T241 fibrosarcoma cells on total tumor volume as a function of time.
- Figure 20 shows the effect of administration of angiostatin protein to mice having implated Lewis lung carcinoma (LM) cells on total tumor volume as a function of time.
- LM Lewis lung carcinoma
- Figure 21 shows the effect of administration of angiostatin protein to mice having implated reticulum cell sarcoma cells on total tumor volume as a function of time.
- Figure 22 shows the effect of administration of angiostatin protein to immunodeficient SCID mice having implated human prostate carcinoma PC-3 cells on total tumor volume as a function of time over a 24 day period.
- Figure 23 shows the effect of administration of angiostatin protein to immunodeficient SCID mice having implated human breast carcinoma MDA-MB cells on total tumor volume as a function of time over a 24 day period.
- FIG. 24 is a schematic representation of cloning of the mouse DNA sequence coding for mouse angiostatin protein derived from mouse plasminogen cDNA.
- the mouse angiostatin encompasses mouse plasminogen kringle regions 1- 4.
- PCR means polymerase chain reaction; PI is the 5'-end oligonucleotide primre for PCR; P2 is the 3'-end oligonucleotide primre for PCR; SS designates the signal sequence; ATG is the translation initiation codon; TAA is the translation stop codon; HA represents the hemagglutinin epitope tag (YPYDVPDYASL); Kl, K2, K3 and K4 represent mouse plasminogen kringle regions 1, 2, 3 and 4 respectively.
- CMV is the cytomegalovirus promoter; T7 is the bacteria phage promoter; PA represents pre-activation proteins; and
- SP6 is the Sp 6 promoter.
- Figure 25 depicts the number of cells as a function of days for non-transfected cells (mock); cells transfected with the vector alone, without the DNA sequence coding for angiostatin (Vector 5), and two angiostatin expressing clones
- Panel (a) represents the results of transfection of T241 cells.
- Panel (b) represents the results of LL2 cells.
- Figure 26 shows the results of culture medium derived from E. coli cells containing the angiostatin clone on cell number.
- Non-transfected cells (mock); cells transfected with the vector alone, without the DNA sequence coding for angiostatin (Vector 5), and three angiostatin expressing clones (AST 25, AST 31 and AST 37).
- Panel (a) represents the results of incubation of culture medium from control (mock) and all angiostatin clones (expressing and non-expressing) on cell number.
- Panel (b) represents the results of incubation of culture medium from control (mock), vector alone (vector 6) and angiostatin clones expressing mouse angiostatin on cell number.
- Panel (c) represents the results of incubation of purified culture medium from control (mock) and angiostatin clones expressing mouse angiostatin on cell number, wherein the culture medium was purified over a lysine-sepharose colume to yield lysine binding components.
- Figure 27 shows the effect on total tumor volume as a function of time of implanting T241 fibrosarcoma cells in mice, where the fibrosarcoma cells have been transfected with a vector containing a DNA sequence coding for angiostatin protein, and where the vector is capable of expressing angiostatin protein.
- "Non-transfected" represents unaltered T241 fibrosarcoma cells implanted in mice.
- Vector 6 represents T241 fibrosarcoma cells transfected with the vector only, which does not contain the DNA sequence coding for angiostatin protein, implanted in mice.
- "Clone 25, Clone 31 and Clone 37” represent three angiostatin-producing clones of T241 fibrosarcoma cells transfected with a vector containg the DNA sequence coding for angiostation protein implanted in mice.
- Figure 28 shows a schematic representation of the structure of human plasminogen and its kringle fragments.
- Human plaminogen is a single chain protein containing 791 amino acids with one side of N-linked glycosylation at Asn289.
- the non-protease region of human plasminogen consisting of the N-terminal 561 amino acids existing in five separate domains, termed kringles as shown in circles (Kl , K2, K3, K4 and K5), along with proteins that separate these structures.
- Each triple disulfide bonded kringle contains 80 amino acids.
- Angiostatin covers the first 4 of these kringle domains (Kl-4), kringle 3 (Kl-3) and kringle 4 (K4) are obtained by digestion of human plasminogen with elastase. The rest of the kringle fragments are recombinant proteins expressed in E. coli.
- SS signal sequence.
- PA preactivation protein.
- Figure 29 shows a SDS-PAGE analysis of purified recombinant and native kringle fragments of plasminogen under reducing conditions.
- B Purified large kringle fragments were stained with Coomassie blue. Kringles 1-4 (lane 2) and kringles 1-3 (lane 3) were obtained by digestion of human plasminogen with elastase and purified by lysine-
- FIG. 30 shows an inhibition of endothelial cell proliferation by recombinant individual kringle fragments of angiostatin. Kringle fragments were assayed on bovine capillary endothelial cells in the presence of 1 ng/ml bFGF for 72 hours.
- A Anti-endothelial cell proliferative effects of two ly sine-binding kringles, rKl and rK4.
- K2 (- ⁇ -) and K3 (- ⁇ -) inhibited BCE cell proliferation in a dose-dependent manner.
- Data represents the mean +/- SEM of triplicates.
- Figure 31 shows an anti-endothelial proliferation activity of large kringle fragments of angiostatin.
- Recombinant K2-3 (-•-) fragments exhibited a less potent inhibition than those of Kl-3 and Kl-4.
- Data represents the mean of three determinations (+/- SEM) as percentages of inhibition.
- Figure 32 shows an additive inhibitory activity of recombinant kringle 2 and kringle 3.
- A The intact fragment of rK2-3 (also see Fig. 31) displayed a weak inhibitory effect only at the concentration of 320 nM. At the same concentration, an additive inhibition was seen when mutant fragments of rK2 cysteine replaced by serine at the position of 169) and K3 (cysteine replaced by serine at the position of 297) were assayed together on BCE cells. Each value represents the mean +/- SEM of triplicates.
- B Schematic structure and amino acid sequence of K2 and K3.
- Figure 33 shows an inhibition of endothelial proliferation by combinatorial kringle fragments.
- the assay was performed with a concentration of 320 nM for each kringle fragment. Values represent the mean of three determinations (+/- SEM) as percentages of inhibition.
- Figure 34 shows an inhibitory activity of angiostatin on endothelial cells after reduction and alkylation.
- Data represents the mean of inhibition +/- SEM of triplicates.
- Figure 35 shows an amino acid sequence alignment of putative kringle domains of human angiostatin. The sequences of four kringle domains were aligned according to their conserved cysteines. Identical and conserved amino acids are shaded. The boxed amino acids in kringle 4 show the positively charged double lysines adjacent to conserved cysteine residues of 22 and 80.
- Figure 36 shows lysine-binding characteristics and reactivity of expressed angiostatin.
- Figure 36A shows a Coomassie stained gel (40 ⁇ l load).
- Figure 36B shows an immunoblot (20 ⁇ l load) of similar gel.
- Lane: 1 shows broth from shake flasks of induced cultures showing angiostatin protein at about 50 kD and a few other proteins. Broth from induced cultures is diluted 1 : 1 with buffer and directly loaded onto lysine-sepharose.
- Lane:2 shows the unbound fraction that passed through the lysine column. All angiostatin protein expressed by P. pastoris binds to the lysine column.
- Lane:3 shows specific elution with 0.2 M amino caproic acid showing that P.
- pastoris expressed angiostatin protein binds lysine and can be purified in a single step to homogeneity over a lysine-sepharose. Also, the P. pastoris expressed angiostatin protein is recognized by a conformationally dependent monoclonal antibody (VAP) raised against kringles 1 to 3.
- VAP conformationally dependent monoclonal antibody
- Figure 37 shows P. pastoris expressed angiostatin protein is seen as a doublet that migrates at 49 kD and 51.5 kD on denatured unreduced SDS-PAGE Coomassie stained gels.
- Panel A and panel B show a Coomassie stained gel and an immunoblot of a similar gel respectively.
- Lane: l shows a purified P. pastoris expressed angiostatin protein.
- Lane:2 shows a purified P. pastoris expressed angiostatin protein incubated in digestion conditions without N-glycanase.
- Lane: 3 shows purified P. pastoris expressed angiostatin protein digested with N-glycanase.
- Figure 38A shows 4 ⁇ g of purified P. pastoris expressed angiostatin protein as a doublet on a Coomassie gel.
- Figure 38B shows that the purified recombinant inhibits BCE proliferation.
- the BCE assay cell counts obtained after 72 hours is shown, in the presence (•) or absence (o) of bFGF, and in the presence of bFGF with PBS as control ( ⁇ ), and in the presence of bFGF with P. pastoris expressed angiostatin protein ( ⁇ ).
- Figure 38C shows that the inhibition is dose dependent.
- Figure 39 shows P. pastoris expressed purified angiostatin was given systemically (subcutaneous) to mice with primary tumors.
- Figures 39A and B show the number of metastases and the lung weights respectively of mice treated daily with saline or P. pastoris expressed angiostatin or plasminogen derived angiostatin protein.
- lungs of mice treated with P. pastoris expressed angiostatin protein or with plasminogen derived angiostatin protein were non-vascularized and metastases were potently suppressed.
- Figure 40 shows that the lungs of mice treated with P. pastoris expressed angiostatin were pink with micrometastases while the lungs of the saline control group were completely covered with vascularized metastases.
- the present invention includes compositions and methods for the detection and treatment of diseases and processes that are mediated by or associated with angiogenesis.
- the composition is angiostatin, which can be isolated from body fluids including, but not limited to, serum, urine and ascites, or synthesized by chemical or biological methods (e.g. cell culture, recombinant gene expression, protein synthesis, and in vitro enzymatic catalysis of plasminogen or plasmin to yield active angiostatin).
- Recombinant techniques include gene amplification from DNA sources using the polymerase chain reaction (PCR), and gene amplification from RNA sources using reverse transcriptase/PCR.
- Angiostatin inhibits the growth of blood vessels into tissues such as unvascularized or vascularized tumors.
- the present invention also encompasses a composition comprising, a vector containing a DNA sequence encoding angiostatin, wherein the vector is capable of expressing angiostatin when present in a cell, a composition comprising a cell containing a vector, wherein the vector contains a DNA sequence encoding angiostatin or fragments or analogs thereof, and wherein the vector is capable of expressing angiostatin when present in the cell, and a method comprising, implanting into a human or non-human animal a cell containing a vector, wherein the vector contains a DNA sequence encoding angiostatin, and wherein the vector is capable of expressing angiostatin when present in the cell.
- the present invention encompasses angiostatin, angiostatin fragments, angiostatin antisera, angiostatin receptor agonists or angiostatin receptor antagonists that are combined with pharmaceutically acceptable excipients, and optionally sustained-release compounds or compositions, such as biodegradable polymers, to form therapeutic compositions.
- the invention includes a composition comprising an antibody that specifically binds to angiostatin, wherein the antibody does not bind to plasminogen.
- the present invention includes a protein designated angiostatin that has a molecular weight of approximately 38 to 45 kilodaltons (kD) that is capable of overcoming the angiogenic activity of endogenous growth factors such as bFGF, in vitro.
- Angiostatin is a protein having a molecular weight of between approximately 38 kilodaltons and 45 kilodaltons as determined by reducing polyacrylamide gel electrophoresis and having an amino acid sequence substantially similar to that of a murine plasminogen fragment beginning at amino acid number 98 of an intact murine plasminogen molecule.
- Numbering of amino acids herein corresponds to the conventioanl system of numbering from the beginning methionine of the plasminogen molecule.
- the term "substantially similar,” when used in reference to angiostatin amino acid sequences, means an amino acid sequence having anti-angiogenic activity and having a molecular weight of approximately 38 kD to 45 kD, which also has a high degree of sequence homology to the protein fragment of mouse plasminogen beginning approximately at amino acid number 98 in mouse plasminogen and weighing 38 kD to 45 kD.
- a high degree of homology means at least approximately 60% amino acid homology, desirably at least approximately 70% amino acid homology, and more desirably at least approximately 80% amino acid homology.
- endothelial inhibiting activity means the capability of a molecule to inhibit angiogenesis in general and, for example, to inhibit the growth of bovine capillary endothelial cells in culture in the presence of fibroblast growth factor.
- the amino acid sequence of the complete murine plasminogen molecule is shown in Figure 1 and in SEQ ID NO: l .
- the sequence for angiostatin protein can begin approximately at amino acid 98.
- Active human angiostatin, howvere, can also begin at a variety of alternative positions.
- the examples demonstrate that genetic constructs encoding active angiostatin protein can begin at amino acid 93 or 102, for example.
- the amino acid sequence of the first 339 amino acids of an angiostatin from mouse is shown in Figure 2, (SEQ ID NO:2), and is compared with the sequences of corresponding plasminogen protein fragments from human (SEQ ID NO: 3, Rhesus monkey (SEQ ID NO:4), porcine (SEQ ID NO:5) and bovine (SEQ ID NO:6) plasminogen. Given that these sequences are identical in well over 50% of their amino acids, it is to be understood that the amino acid sequence of the angiostatin is substantially similar among species.
- the total number of amino acids in angiostatin is not known precisely but is defined by the molecular weight of the active molecule.
- the amino acid sequence of the angiostatin of the present invention may vary depending upon from which species the plasminogen molecule is derived. Thus, although the angiostatin of the present invention that is derived from human plasminogen has a slightly different sequence than angiostatin derived from mouse, it has anti-angiogenic activity as shown in a mouse tumor model.
- Angiostatin has been shown to be capable of inhibiting the growth of endothelial cells in vitro. Angiostatin does not inhibit the growth of cell lines derived from other cell types.
- angiostatin has no effect on Lewis lung carcinoma cell lines, mink lung epithelium, 3T3 fibroblasts, bovine aortic smooth muscle cells, bovine retinal pigment epithelium, MDCk cells (canine renal epithelium), WI38 cells (human fetal lung fibroblasts) EFN cells (murine fetal fibroblasts) and LM cells
- Endogenous angiostatin in a tumor bering mouse is effective at inhibiting metastases at a systemic concentration of approximately 10 mg angiostatin/kg body weight.
- Angiostatin has a specific three dimensional conformation that is defined by the kringle regions of the plasminogen molecule. (Robbins, K.C., "The plasminogen- plasmin enzyme system” Hemostasis and Thrombosis, Basic Principles and Practice, 2nd Edition, ed. by Colman, R.W. et al. J.B. Lippincott Company, pp.
- kringle regions which are conformationally related motifs and have substantial sequence homology, in the NH2 terminal portion of the plasminogen molecule.
- the three dimensional conformation of functional angiostatin is believed to encompass plasminogen kringle regions 1 through 5.
- Each kringle region of the plasminogen molecule contains approximately 80 amino acids and contains 3 disulfide bonds.
- This cysteine motif is known to exist in other biologically active proteins. These proteins include, but are not limited to, prothrombin, hepatocyte growth factor, scatter factor and macrophage stimulating protein.
- MSP human macrophage stimulating protein
- the present invention also includes the detection of the angiostatin in body fluids and tissues for the purpose of diagnosis or prognosis of diseases such as cancer.
- the present invention also includes the detection of angiostatin binding sites and receptors in cells and tissues.
- the present invention also includes methods of treating or preventing angiogenic diseases and processes including, but not limited to, arthritis and tumors by stimulating the production of angiostatin, and/or by administering substantially purified angiostatin, or angiostatin agonists or antagonists, and/or angiostatin antisera or antisera directed against angiostatin antisera to a patient.
- Additional treatment methods include administration of angiostatin, angiostatin fragments, angiostatin analogs, angiostatin antisera, or angiostatin receptor agonists and antagonists linked to cytotoxic agents. It is to be understood that the angiostatin can be animal or human in origin.
- Angiostatin can also be produced synthetically by chemical reaction or by recombinant techniques in conjunction with expression systems. Angiostatin can also be produced by enzymatically cleaving isolated plasminogen or plasmin to generate proteins having anti-angiogenic activity. Angiostatin may also be produced by compounds that mimic the action of endogenous enzymes that cleave plasminogen to angiostatin. Angiostatin production may also be modulated by compounds that affect the activity of plasminogen cleaving enxymes. Passive antibody therapy using antibodies that specifically bind angiostatin can be employed to modulate angiogenic-dependent processes such as reproduction, development, and wound healing and tissue repair. In addition, antisera directed to the Fab regions of angiostatin antibodies can be administered to block the ability of endogenous angiostatin antisera to bind angiostatin.
- the present invention also encompasses gene therapy whereby the gene encoding angiostatin is regulated in a patient.
- gene therapy encompasses incorporation of DNA sequences into somatic cells or germ line cells for use in either ex vivo or in vivo therapy. Gene therapy functions to replace genes, augment normal or abnormal gene function, and to combat infectious diseases and other pathologies.
- Strategies for treating these medical problems with gene therapy include therapeutic strategies such as identifying the defective gene and then adding a functional gene to either replace the function of the defective gene or to augment a slightly functional gene; or prophylactic strategies, such as adding a gene for the product protein that will treat the condition or that will make the tissue or organ more susceptible to a treatment regimen.
- a gene such as angiostatin may be placed in a patient and thus prevent occurrence of angiogenesis; or a gene that makes tumor cells more susceptible to radiation could be inserted and then radiation of the tumor would cause increased killing of the tumor cells.
- Gene transfer methods for gene therapy fall into three broad categories-physical (e.g., electroporation, direct gene transfer and particle bombardment), chemical (lipid-based carriers, or other non-viral vectors) and biological (virus- derived vector and receptor uptake).
- non-viral vectors may be used which include liposomes coated with DNA.
- liposome/DNA complexes may be directly injected intravenously into the patient. It is believed that the liposome/DNA complexes are concentrated in the liver where they deliver the DNA to macrophages and Kupffer cells. These cells are long lived and thus provide long term expression of the delivered DNA.
- vectors or the "naked" DNA of the gene may be directly injected into the desired organ, tissue or tumor for targeted delivery of the therapeutic DNA.
- Gene therapy methodologies can also be described by delivery site. Fundamental ways to deliver genes include ex vivo gene transfer, in vivo gene transfer, and in vitro gene transfer.
- ex vivo gene transfer cells are taken from the patient and grown in cell culture. The DNA is transfected into the cells, the transfected cells are expanded in number and then reimplanted in the patient.
- in vitro gene transfer the transformed cells are cells growing in culture, such as tissue culture cells, and not particular cells from a particular patient. These "laboratory cells" are transfected, the transfected cells are selected and expanded for either implantation into a patient or for other uses.
- In vivo gene transfer involves introducing the DNA into the cells of the patient when the cells are within the patient.
- Methods include using virally mediated gene transfer using a noninfectious virus to deliver the gene in the patient or injecting naked DNA into a site in the patient and the DNA is taken up by a percentage of cells in which the gene product protein is expressed. Additionally, the other methods described herein, such as use of a "gene gun,” may be used for in vitro insertion of angiostatin DNA or angiostatin regulatory sequences. Chemical methods of gene therapy may involve a lipid based compound, not necessarily a liposome, to ferry the DNA across the cell membrane. Lipofectins or cytofectins, lipid- based positive ions that bind to negatively charged DNA, make a complex that can cross the cell membrane and provide the DNA into the interior of the cell.
- Another chemical method uses receptor-based endocytosis, which involves binding a specific ligand to a cell surface receptor and enveloping and transporting it across the cell membrane.
- the ligand binds to the DNA and the whole complex is transported into the cell.
- the ligand gene complex is injected into the blood stream and then target cells that have the receptor will specifically bind the ligand and transport the ligand-DNA complex into the cell.
- viral vectors to insert genes into cells.
- altered retrovirus vectors have been used in ex vivo methods to introduce genes into peripheral and tumor-infiltrating lymphocytes, hepatocytes, epidermal cells, myocytes, or other somatic cells. These altered cells are then introduced into the patient to provide the gene product from the inserted DNA.
- Viral vectors have also been used to insert genes into cells using in vivo protocols. To direct tissue-specific expression of foreign genes, cis-acting regulatory elements or promoters that are known to be tissue specific can be used. Alternatively, this can be achieved using in situ delivery of
- DNA or viral vectors to specific anatomical sites in vivo were achieved by implanting in vitro transduced endothelial cells in chosen sites on arterial walls. The virus infected surrounding cells which also expressed the gene product.
- a viral vector can be delivered directly to the in vivo site, by a catheter for example, thus allowing only certain areas to be infected by the virus, and providing long-term, site specific gene expression.
- retrovirus vectors has also been demonstrated in mammary tissue and hepatic tissue by injection of the altered virus into blood vessels leading to the organs.
- Viral vectors that have been used for gene therapy protocols include but are not limited to, retroviruses, other RNA viruses such as poliovirus or Sindbis virus , adenovirus, adeno-associated virus, herpes viruses, SV 40, vaccinia and other DNA viruses.
- Replication-defective murine retroviral vectors are the most widely utilized gene transfer vectors.
- Murine leukemia retroviruses are composed of a single strand RNA complexed with a nuclear core protein and polymerase
- retroviral vector systems exploit the fact that a minimal vector containing the 5' and 3' LTRs and the packaging signal are sufficient to allow vector packaging, infection and integration into target cells providing that the viral structural proteins are supplied in trans in the packaging cell line. Fundamental advantages of retroviral vectors for gene transfer include efficient infection and gene expression in most cell types, precise single copy vector integration into target cell chromosomal DNA, and ease of manipulation of the retroviral genome.
- the adenovirus is composed of linear, double stranded
- Adenoviral-based vectors will express gene product proteins at high levels.
- Adenoviral vectors have high efficiencies of infectivity, even with low titers of virus. Additionally, the virus is fully infective as a cell free virion so injection of producer cell lines are not necessary.
- Another potential advantage to adenoviral vectors is the ability to achieve long term expression of heterologous genes in vivo.
- DNA delivery include fusogenic lipid vesicles such as liposomes or other vesicles for membrane fusion, lipid particles of DNA incorporating cationic lipid such as lipofectin, polylysine-mediated transfer of DNA, direct injection of DNA, such as microinjection of DNA into germ or somatic cells, pneumatically delivered DNA-coated particles, such as the gold particles used in a "gene gun," and inorganic chemical approaches such as calcium phosphate transfection.
- Another method, ligand- mediated gene therapy involves complexing the DNA with specific ligands to form ligand-DNA conjugates, to direct the DNA to a specific cell or tissue.
- Non-integration of the transfected DNA would allow the transfection and expression of gene product proteins in terminally differentiated, non-proliferative tissues for a prolonged period of time without fear of mutational insertions, deletions, or alterations in the cellular or mitochondrial genome. Long-term, but not necessarily permanent, transfer of therapeutic genes into specific cells may provide treatments for genetic diseases or for prophylactic use.
- the DNA could be reinjected periodically to maintain the gene product level without mutations occurring in the genomes of the recipient cells.
- Non-integration of exogenous DNAs may allow for the presence of several different exogenous DNA constructs within one cell with all of the constructs expressing various gene products.
- Particle-mediated gene transfer methods were first used in transforming plant tissue. With a particle bombardment device, or "gene gun," a motive force is generated to accelerate DNA-coated high density particles (such as gold or tungsten) to a high velocity that allows penetration of the target organs, tissues or cells. Particle bombardment can be used in in vitro systems, or with ex vivo or in vivo techniques to introduce DNA into cells, tissues or organs. Electroporation for gene transfer uses an electrical current to make cells or tissues susceptible to electroporation- mediated gene transfer. A brief electric impulse with a given field strength is used to increase the permeability of a membrane in such a way that DNA molecules can penetrate into the cells. This technique can be used in in vitro systems, or with ex vivo or in vivo techniques to introduce DNA into cells, tissues or organs.
- a motive force is generated to accelerate DNA-coated high density particles (such as gold or tungsten) to a high velocity that allows penetration of the target organs, tissues or cells.
- Carrier mediated gene transfer in vivo can be used to transfect foreign DNA into cells.
- the carrier-DNA complex can be conveniently introduced into body fluids or the bloodstream and then site specifically directed to the target organ or tissue in the body.
- Both liposomes and polycations, such as polylysine, lipofectins or cytofectins, can be used.
- Liposomes can be developed which are cell specific or organ specific and thus the foreign DNA carried by the liposome will be taken up by target cells. Injection of immunoliposomes that are targeted to a specific receptor on certain cells can be used as a convenient method of inserting the DNA into the cells bearing the receptor.
- Another carrier system that has been used is the asialoglycoportein/polylysine conjugate system for carrying DNA to hepatocytes for in vivo gene transfer.
- the transfected DNA may also be complexed with other kinds of carriers so that the DNA is carried to the recipient cell and then resides in the cytoplasm or in the nucleoplasm.
- DNA can be coupled to carrier nuclear proteins in specifically engineered vesicle complexes and carried directly into the nucleus.
- Gene regulation of angiostatin may be accomplished by administering compounds that bind to the angiostatin gene, or control regions associated with the angiostatin gene, or its corresponding RNA transcript to modify the rate of transcription or translation.
- cells transfected with a DNA sequence encoding angiostatin may be administered to a patient to provide an in vivo source of angiostatin.
- cells may be transfected with a vector containing a nucleic acid sequence encoding angiostatin.
- vector means a carrier that can contain or associate with specific nucleic acid sequences, which functions to transport the specific nucleic acid sequences into a cell.
- vectors include plasmids and infective microorganisms such as viruses, or non- viral vectors such as ligand-DNA conjugates, liposomes, lipid-DNA complexes. It may be desirable that a recombinant DNA molecule comprising an angiostatin DNA sequence is operatively linked to an expression control sequence to form an expression vector capable of expressing angiostatin.
- the transfected cells may be cells derived from the patient's normal tissue, the patient's diseased tissue, or may be non-patient cells.
- tumor cells removed from a patient can be transfected with a vector capable of expressing the angiostatin protein of the present invention, and re-introduced into the patient.
- the transfected tumor cells produce angiostatin levels in the patient that inhibit the growth of the tumor.
- Patients may be human or non-human animals.
- Cells may also be transfected by non-vector, or physical or chemical methods known in the art such as electroporation, ionoporation, or via a "gene gun.”
- angiostatin DNA may be directly injected, without the aid of a carrier, into a patient.
- angiostatin DNA may be injected into skin, muscle or blood.
- the gene therapy protocol for transfecting angiostatin into a patient may either be through integration of the angiostatin DNA into the genome of the cells, into minichromosomes or as a separate replicating or non- replicating DNA construct in the cytoplasm or nucleoplasm of the cell.
- Angiostatin expression may continue for a long- period of time or may be reinjected periodically to maintain a desired level of the angiostatin protein in the cell, the tissue or organ or a determined blood level.
- Angiostatin can be isolated on an HPLC C4 column (see Table 3).
- the angiostatin protein is eluted at 30 to 35% in an acetonitrile gradient.
- PAGE polyacrylamide gel electrophoresis
- a growing primary tumor is associated with the release into the blood stream of specific inhibitor(s) of endothelial cell proliferation, including angiostatin which can suppress angiogenesis within a metastasis and thereby inhibit the growth of the metastasis itself.
- the source of the angiostatin associated with the primary tumor is not known.
- the compound may be produced by degradation of plasminogen by a specific protease, or angiostatin could be produced by expression of a specific gene coding for angiostatin.
- the angiogenic phenotype of a primary tumor depends on production of angiogenic proteins in excess of endothelial cell inhibitors which are elaborated by normal cells, but are believed to be down-regulated during transformation to neoplasia.
- angiostatin While production of angiostatin may be down- regulated in an individual tumor cell relative to production by its parent cell type, the total amount of inhibitor elaborated by the whole tumor may be sufficient to enter the circulation and suppress endothelial growth at remote sites of micrometastases.
- Angiostatin remains in the circulation for a significantly longer time than the angiogenic protein(s) released by a primary tumor.
- the angiogenic proteins appear to act locally, whereas angiostatin acts globally and circulates in the blood with a relatively long half-life.
- the half-life of the angiostatin is approximately 12 hours to 5 days.
- angiogenic proteins e.g., aFGF, bFGF, VEGF, IL-8, GM-CSF, etc.
- angiogenic proteins e.g., aFGF, bFGF, VEGF, IL-8, GM-CSF, etc.
- angiogenic proteins e.g., aFGF, bFGF, VEGF, IL-8, GM-CSF, etc.
- angiogenic proteins must be produced in an amount sufficient to overcome the action of endothelial cell inhibitor (inhibitors of angiogenesis) for a primary tumor to continue to expand its population.
- a second tumor implant e.g., into the subcutaneous space, or into the cornea, or intravenously to the lung
- the primary tumor will not be able to suppress the secondary tumor (because angiogenesis in the secondary tumor will already be well underway).
- the inhibitors may have an equivalent inhibiting effect on each other.
- the angiostatin of the present invention can be:
- angiostatin Used as the basis to analyze serum and urine of cancer patients for similar angiostatic molecules. It is contemplated as part of the present invention that angiostatin can be isolated from a body fluid such as blood or urine of patients or the angiostatin can be produced by recombinant DNA methods or synthetic protein chemical methods that are well known to those of ordinary skill in the art. Protein purification methods are well known in the art and a specific example of a method for purifying angiostatin, and assaying for inhibitor activity is provided in the examples below. Isolation of human endogenous angiostatin is accomplished using similar techniques.
- One example of a method of producing angiostatin using recombinant DNA techniques entails the steps of (1) identifying and purifying angiostatin as discussed above, and as more fully described below, (2) determining the N-terminal amino acid sequence of the purified inhibitor, (3) synthetically generating 5' and 3' DNA oligonucleotide primers for the angiostatin sequence, (4) amplifying the angiostatin gene sequence using polymerase, (5) inserting the amplified sequence into an appropriate vector such as an expression vector, (6) inserting the gene containing vector into a microorganism or other expression system capable of expressing the inhibitor gene, and (7) isolating the recombinantly produced inhibitor.
- Appropriate vectors include viral, bacterial and eukaryotic (such as yeast) expression vectors. The above techniques are more fully described in laboratory manuals such as "Molecular Cloning:
- the gene for angiostatin may also be isolated from cells or tissue (such as tumor cells) that express high levels of angiostatin by (1) isolating messenger RNA from the tissue, (2) using reverse transcriptase to generate the corresponding
- angiostatin DNA sequence and then (3) using the polymerase chain reaction (PCR) with the appropriate primers to amplify the DNA sequence coding for the active angiostatin amino acid sequence.
- PCR polymerase chain reaction
- Yet another method of producing angiostatin, or biologically active fragments thereof, is by protein synthesis. Once a biologically active fragment of an angiostatin is found using the assay system described more fully below, it can be sequenced, for example by automated protein sequencing methods. Alternatively, once the gene or DNA sequence which codes for angiostatin is isolated, for example by the methods described above, the DNA sequence can be determined using manual or automated sequencing methods well know in the art. The nucleic acid sequence in turn provides information regarding the amino acid sequence.
- the biologically active fragment is generated by specific methods, such as tryptic digests, or if the fragment is N-terminal sequenced, the remaining amino acid sequence can be determined from the corresponding DNA sequence.
- the fragment can be synthesized by techniques well known in the art, as exemplified by "Solid Phase Protein Synthesis: A Practical Approach” E. Atherton and R.C. Sheppard, IRL Press, Oxford, England. Similarly, multiple fragments can be synthesized which are subsequently linked together to form larger fragments. These synthetic protein fragments can also be made with amino acid substitutions at specific locations to test for agonistic and antagonistic activity in vitro and in vivo.
- Protein fragments that possess high affinity binding to tissues can be used to isolate the angiostatin receptor on affinity columns. Isolation and purification of the angiostatin receptor is a fundamental step towards elucidating the mechanism of action of angiostatin. Isolation of an angiostatin receptor and identification of angiostatin agonists and antagonists will facilitate development of drugs to modulate the activity of the angiostatin receptor, the final pathway to biological activity. Isolation of the receptor enables the construction of nucleotide probes to monitor the location and synthesis of the receptor, using in situ and solution hybridization technology.
- the gene for the angiostatin receptor can be isolated, incorporated into an expression vector and transfected into cells, such as patient tumor cells to increase the ability of a cell type, tissue or tumor to bind angiostatin and inhibit local angiogenesis.
- Angiostatin is effective in treating diseases or processes that are mediated by, or involve, angiogenesis.
- the present invention includes the method of treating an angiogenesis mediated disease with an effective amount of angiostatin, or a biologically active fragment thereof, or combinations of angiostatin fragmetns that collectively possess anti-angiogenic activity, or angiostatin agonists and antagonists.
- the angiogenesis mediated diseases include, but are not limited to, solid tumors; blood born tumors such as leukemias; tumor metastasis; benign tumors, for example hemangiomas, acoustic neuromas, neurofibromas, trachomas, and pyogenic granulomas; rheumatoid arthritis; psoriasis; ocular angiogenic diseases, for example, diabetic retinopathy, retinopathy of prematurity, macular degeneration, corneal graft rejection, neovascular glaucoma, retrolental fibroplasia, rubeosis; Osler- Webber Syndrome; myocardial angiogenesis; plaque neovascularization; telangiectasia; hemophiliac joints; angiofibroma; and wound granulation.
- solid tumors such as leukemias
- tumor metastasis such as leukemias
- benign tumors for example hemangiomas, acoustic
- Angiostatin is useful in the treatment of disease of excessive or abnormal stimulation of endothelial cells. These diseases include, but are not limited to, intestinal adhesions, Crohn's disease, atherosclerosis, scleroderma, and hypertrophic scars, i.e., keloids. Angiostatin can be used as a birth control agent by preventing vascularization required for embryo implantation. Angiostatin is useful in the treatment of diseases that have angiogenesis as a pathologic consequence such as cat scratch disease ⁇ Rochele minalia quintosa) and ulcers ⁇ Helicobacter pylori).
- the synthetic protein fragments of angiostatin have a variety of uses.
- the protein that binds to the angiostatin receptor with high specificity and avidity is radiolabeled and employed for visualization and quantitation of binding sites using autoradiographic and membrane binding techniques. This application provides important diagnostic and research tools. Knowledge of the binding properties of the angiostatin receptor facilitates investigation of the transduction mechanisms linked to the receptor.
- angiostatin proteins with short lived isotopes enables visualization of receptor binding sites in vivo using positron emission tomography or other modern radiographic techniques to locate tumors with angiostatin binding sites.
- Systematic substitution of amino acids within these synthesized proteins yields high affinity protein agonists and antagonists to the angiostatin receptor that enhance or diminish angiostatin binding to its receptor.
- Such agonists are used to suppress the growth of micrometastases, thereby limiting the spread of cancer.
- Antagonists to angiostatin are applied in situations of inadequate vascularization, to block the inhibitory effects of angiostatin and promote angiogenesis. For example, this treatment may have therapeutic effects to promote wound healing in diabetics.
- Angiostatin proteins are employed to develop affinity columns for isolation of the angiostatin receptor from cultured tumor cells. Isolation and purification of the angiostatin receptor is followed by amino acid sequencing. Using this information the gene or genes coding for the angiostatin receptor can be identified and isolated. Next, cloned nucleic acid sequences are developed for insertion into vectors capable of expressing the receptor. These techniques are well known to those skilled in the art. Transfection of the nucleic acid sequence(s) coding for angiostatin receptor into tumor cells, and expression of the receptor by the transfected tumor cells enhances the responsiveness of these cells to endogenous or exogenous angiostatin and thereby decreasing the rate of metastatic growth.
- Cytotoxic agents such as ricin, are linked to angiostatin, and high affinity angiostatin protein fragments, thereby providing a tool for destruction of cells that bind angiostatin. These cells may be found in many locations, including but not limited to, micrometastases and primary tumors. Proteins linked to cytotoxic agents are infused in a manner designed to maximize delivery to the desired location. For example, ricin- linked high affinity angiostatin fragments are delivered through a cannula into vessels supplying the target site or directly into the target. Such agents are also delivered in a controlled manner through osmotic pumps coupled to infusion cannulae. A combination of angiostatin antagonists may be co- applied with stimulators of angiogenesis to increase vascularization of tissue.
- Angiostatin may be used in combination with other compositions and procedures for the treatment of diseases. For example, a tumor may be treated conventionally with surgery, radiation or chemotherapy combined with angiostatin and then angiostatin may be subsequently administered to the patient to extend the dormancy of micrometastases and to stabilize and inhibit the growth of any residual primary tumor. Additionally, angiostatin, angiostatin fragments, angiostatin antisera, angiostatin receptor agonists, angiostatin receptor antagonists, or combinations thereof, are combined with pharmaceutically acceptable excipients, and optionally sustained-release matrix, such as biodegradable polymers, to form therapeutic compositions.
- pharmaceutically acceptable excipients such as biodegradable polymers
- a sustained-release matrix is a matrix made of materials, usually polymers, which are degradable by enzymatic or acid/base hydrolysis or by dissolution. Once inserted into the body, the matrix is acted upon by enzymes and body fluids.
- the sustained-release matrix desirably is chosen from biocompatible materials such as liposomes, polylactides (polylactic acid), polyglycolide (polymer of glycolic acid), polylactide co-glycolide (co-polymers of lactic acid and glycolic acid) polyanhydrides, poly(ortho)esters, polyproteins, hyaluronic acid, collagen, chondroitin sulfate, carboxylic acids, fatty acids, phospholipids, polysaccharides, nucleic acids, polyamino acids, amino acids such as phenylalanine, tyrosine, isoleucine, polynucleotides, polyvinyl propylene, polyvinylpyrrolidone and silicone.
- biocompatible materials such as liposomes, polylactides (polylactic acid), polyglycolide (polymer of glycolic acid), polylactide co-glycolide (co-polymers of lactic acid and glycolic acid) polyan
- a preferred biodegradable matrix is a matrix of one of either polylactide, polyglycolide, or polylactide co-glycolide (co-polymers of lactic acid and glycolic acid).
- the angiogenesis-modulating therapeutic composition of the present invention may be a solid, liquid or aerosol and may be administered by any known route of administration.
- solid therapeutic compositions include pills, creams, and implantable dosage units. The pills may be administered orally, the therapeutic creams may be administered topically.
- the implantable dosage unitst may be administered locally, for example at a tumor site, or which may be implanted for systemic release of the therapeutic angiogenesis-modulating composition, for example subcutaneously.
- liquid composition include formulations adapted for injection subcutaneously, intravenously, intraarterially, and formulations for topical and intraocular administration.
- aersol formulation include inhaler formulation for administration to the lungs.
- the angiostatin of the present invention also can be used to generate antibodies that are specific for the inhibitor and its receptor.
- the antibodies can be either polyclonal antibodies or monoclonal antibodies. These antibodies that specifically bind to the angiostatin or angiostatin receptors can be used in diagnostic methods and kits that are well known to those of ordinary skill in the art to detect or quantify the angiostatin or angiostatin receptors in a body fluid or tissue. Results from these tests can be used to diagnose or predict the occurrence or recurrence of a cancer and other angiogenic mediated diseases.
- the angiostatin also can be used in a diagnostic method and kit to detect and quantify antibodies capable of binding angiostatin.
- kits would permit detection of circulating angiostatin antibodies which indicates the spread of micrometastases in the presence of angiostatin secreted by primary tumors in situ. Patients that have such circulating anti-angiostatin antibodies may be more likely to develop multiple tumors and cancers, and may be more likely to have recurrences of cancer after treatments or periods of remission.
- the Fab fragments of these anti-angiostatin antibodies may be used as antigens to generate anti-angiostatin Fab-fragment antisera which can be used to neutralize anti-angiostatin antibodies. Such a method would reduce the removal of circulating angiostatin by anti-angiostatin antibodies, thereby effectively elevating circulating angiostatin levels.
- Another aspect of the present invention is a method of blocking the action of excess endogenous angiostatin. This can be done by passively immunizing a human or animal with antibodies specific for the undesired angiostatin in the system. This treatment can be important in treating abnormal ovulation, menstruation and placentation, and vasculogenesis. This provides a useful tool to examine the effects of angiostatin removal on metastatic processes.
- the Fab fragment of angiostatin antibodies contains the binding site for angiostatin. This fragment is isolated from angiostatin antibodies using techniques known to those skilled in the art.
- the Fab fragments of angiostatin antisera are used as antigens to generate production of anti-Fab fragment serum. Infusion of this antiserum against the Fab fragments of angiostatin prevents angiostatin from binding to angiostatin antibodies.
- Therapeutic benefit is obtained by neutralizing endogenous anti-angiostatin antibodies by blocking the binding of angiostatin to the Fab fragments of anti-angiostatin.
- the net effect of this treatment is to facilitate the ability of endogenous circulating angiostatin to reach target cells, thereby decreasing the spread of metastases.
- the present invention is contemplated to include any derivatives of the angiostatin that have endothelial inhibitory activity.
- the present invention includes the entire angiostatin protein, derivatives of the angiostatin protein and biologically-active fragments of the angiostatin protein. These include proteins with angiostatin activity that have amino acid substitutions or have sugars or other molecules attached to amino acid functional groups.
- the present invention also includes genes that code for angiostatin and the angiostatin receptor, and to proteins that are expressed by those genes.
- the proteins and protein fragments with the angiostatin activity described above can be provided as isolated and substantially purified proteins and protein fragments in pharmaceutically acceptable formulations using formulation methods known to those of ordinary skill in the art. These formulations can be administered by standard routes. In general, the combinations may be administered by the topical, tr an s de rm al , i n trap e ri to n e al , i n trac ran i al , intracerebroventricular, intracerebral, intravaginal, intrauterine, oral, rectal or parenteral (e.g., intravenous, intraspinal, subcutaneous or intramuscular) route.
- parenteral e.g., intravenous, intraspinal, subcutaneous or intramuscular route.
- angiostatin may be incorporated into biodegradable polymers allowing for sustained release of the compound, the polymers being implanted in the vicinity of where drug delivery is desired, for example, at the site of a tumor or implanted so that the angiostatin is slowly released systemically.
- Osmotic minipumps may also be used to provide controlled delivery of high concentrations of angiostatin through cannulae to the site of interest, such as directly into a metastatic growth or into the vascular supply to that tumor.
- the biodegradable polymers and their use are described, for example, in detail in Brem et al., J. Neurosurg. 74:441-446 (1991), which is hereby incorporated by reference in its entirety.
- the dosage of the angiostatin of the present invention will depend on the disease state or condition being treated and other clinical factors such as weight and condition of the human or animal and the route of administration of the compound. For treating humans or animals, between approximately 0.5 mg/kilogram to 500 mg/kilogram of the angiostatin can be administered. Depending upon the half-life of the angiostatin in the particular animal or human, the angiostatin can be administered between several times per day to once a week. It is to be understood that the present invention has application for both human and veterinary use. The methods of the present invention contemplate single as well as multiple administrations, given either simultaneously or over an extended period of time.
- the angiostatin formulations include those suitable for oral, rectal, ophthalmic (including intravitreal or intracameral), nasal, topical (including buccal and sublingual), intrauterine, vaginal or parenteral (including subcutaneous, intraperitoneal, intramuscular, intravenous, intradermal, intracranial, intratracheal, and epidural) administration.
- the angiostatin formulations may conveniently be presented in unit dosage form and may be prepared by conventional pharmaceutical techniques. Such techniques include the step of bringing into association the active ingredient and the pharmaceutical carrier(s) or excipient(s). In general, the formulations are prepared by uniformly and intimately bringing into association the active ingredient with liquid carriers or finely divided solid carriers or both, and then, if necessary, shaping the product.
- Formulations suitable for parenteral administration include aqueous and non-aqueous sterile injection solutions which may contain anti-oxidants, buffers, bacteriostats and solutes which render the formulation isotonic with the blood of the intended recipient; and aqueous and non-aqueous sterile suspensions which may include suspending agents and thickening agents.
- the formulations may be presented in unit- dose or multi-dose containers, for example, sealed ampules and vials, and may be stored in a freeze-dried (lyophilized) condition requiring only the addition of the sterile liquid carrier, for example, water for injections, immediately prior to use.
- Extemporaneous injection solutions and suspensions may be prepared from sterile powders, granules and tablets of the kind previously described.
- Preferred unit dosage formulations are those containing a daily dose or unit, daily sub-dose, or an appropriate fraction thereof, of the administered ingredient.
- the formulations of the present invention may include other agents conventional in the art having regard to the type of formulation in question.
- cytotoxic agents may be incorporated or otherwise combined with angiostatin proteins, or biologically functional protein fragements thereof, to provide dual therapy to the patient.
- Angiogenesis inhibiting proteins of the present invention can be synthesized in a standard microchemical facility and purity checked with HPLC and mass spectrophotometry. Methods of protein synthesis, HPLC purification and mass spectrophotometry are commonly known to those skilled in these arts. Angiostatin proteins and angiostatin receptors proteins are also produced in recombinant E. coli or yeast expression systems, and purified with column chromatography.
- angiostatin molecule can be synthesized for use in several applications including, but not limited to the following; as antigens for the development of specific antisera, as agonists and antagonists active at angiostatin binding sites, as proteins to be linked to, or used in combination with, cytotoxic agents for targeted killing of cells that bind angiostatin.
- the amino acid sequences that comprise these proteins are selected on the basis of their position on the exterior regions of the molecule and are accessible for binding to antisera.
- the amino and carboxyl termini of angiostatin, as well as the mid-region of the molecule are represented separately among the fragments to be synthesized.
- Protein sequences are compared to known sequences using protein sequence databases such as GenBank, Brookhaven Protein, SWISS-PROT, and PIR to determine potential sequence homologies. This information facilitates elimination of sequences that exhibit a high degree of sequence homology to other molecules, thereby enhancing the potential for high specificity in the development of antisera, agonists and antagonists to angiostatin.
- Angiostatin and angiostatin derived proteins can be coupled to other molecules using standard methods.
- the amino and carboxyl termini of angiostatin both contain tyrosine and lysine residues and are isotopically and nonisotopically labeled with many techniques, for example radiolabeling using conventional techniques (tyrosine residues- chloramine T, iodogen, lactoperoxidase; lysine residues- Bolton-Hunter reagent). These coupling techniques are well known to those skilled in the art.
- tyrosine or lysine is added to fragments that do not have these residues to facilitate labeling of reactive amino and hydroxyl groups on the protein.
- the coupling technique is chosen on the basis of the functional groups available on the amino acids including, but not limited to amino, sulfhydral, carboxyl, amide, phenol, and imidazole.
- Various reagents used to effect these couplings include among others, glutaraldehyde, diazotized benzidine, carbodiimide, and p-benzoquinone.
- Angiostatin proteins are chemically coupled to isotopes, enzymes, carrier proteins, cytotoxic agents, fluorescent molecules, chemiluminescent, bioluminescent and other compounds for a variety of applications.
- the efficiency of the coupling reaction is determined using different techniques appropriate for the specific reaction. For example, radiolabeling of an angiostatin protein with I is accomplished using chloramine T and Na I of high specific activity. The reaction is terminated with sodium metabisulfite and the mixture is desalted on disposable columns. The labeled protein is eluted from the column and fractions are collected. Aliquots are removed from each fraction and radioactivity measured in a gamma counter. In this manner, the unreacted Na I is separated from the labeled angiostatin protein.
- the protein fractions with the highest specific radioactivity are stored for subsequent use such as analysis of the ability to bind to angiostatin antisera.
- Another application of protein conjugation is for production of polyclonal antisera.
- angiostatin proteins containing lysine residues are linked to purified bovine serum albumin using glutaraldehyde. The efficiency of the reaction is determined by measuring the incorporation of radiolabeled protein. Unreacted glutaraldehyde and protein are separated by dialysis. The conjugate is stored for subsequent use.
- Antiserum against angiostatin, angiostatin analogs, protein fragments of angiostatin and the angiostatin receptor can be generated. After protein synthesis and purification, both monoclonal and polyclonal antisera are raised using established techniques known to those skilled in the art. For example, polyclonal antisera may be raised in rabbits, sheep, goats or other animals. Angiostatin proteins conjugated to a carrier molecule such as bovine serum albumin, or angiostatin itself, is combined with an adjuvant mixture, emulsified and injected subcutaneously at multiple sites on the back, neck, flanks, and sometimes in the footpads. Booster injections are made at regular intervals, such as every 2 to 4 weeks.
- Blood samples are obtained by venipuncture, for example using the marginal ear veins after dilation, approximately 7 to 10 days after each injection.
- the blood samples are allowed to clot overnight at 4C and are centrifuged at approximately 2400 X g at 4C for about 30 minutes.
- the serum is removed, aliquoted, and stored at 4C for immediate use or at -20 to
- All serum samples from generation of polyclonal antisera or media samples from production of monoclonal antisera are analyzed for determination of antibody titer. Titer is established through several means, for example, using dot blots and density analysis, and also with precipitation of radiolabeled protein-antibody complexes using protein A, secondary antisera, cold ethanol or charcoal-dextran followed by activity measurement with a gamma counter.
- the highest titer antisera are also purified on affinity columns which are commercially available.
- Angiostatin proteins are coupled to the gel in the affinity column.
- Antiserum samples are passed through the column and anti-angiostatin antibodies remain bound to the column. These antibodies are subsequently eluted, collected and evaluated for determination of titer and specificity.
- the highest titer angiostatin antisera is tested to establish the following; a) optimal antiserum dilution for highest specific binding of the antigen and lowest non-specific binding, b) the ability to bind increasing amounts of angiostatin protein in a standard displacement curve, c) potential cross-reactivity with related proteins and proteins, including plasminogen and also angiostatin of related species, d) ability to detect angiostatin proteins in extracts of plasma, urine, tissues, and in cell culture media.
- Kits for measurement of angiostatin, and the angiostatin receptor are also contemplated as part of the present invention.
- Antisera that possess the highest titer and specificity and can detect angiostatin proteins in extracts of plasma, urine, tissues, and in cell culture media are further examined to establish easy to use kits for rapid, reliable, sensitive, and specific measurement and localization of angiostatin.
- assay kits include but are not limited to the following techniques; competitive and non-competitive assays, radioimmunoassay, bioluminescence and chemiluminescence assays, fluorometric assays, sandwich assays, immunoradiometric assays, dot blots, enzyme linked assays including ELISA, microtiter plates, antibody coated strips or dipsticks for rapid monitoring of urine or blood, and immunocytochemistry.
- competitive and non-competitive assays radioimmunoassay, bioluminescence and chemiluminescence assays, fluorometric assays, sandwich assays, immunoradiometric assays, dot blots, enzyme linked assays including ELISA, microtiter plates, antibody coated strips or dipsticks for rapid monitoring of urine or blood, and immunocytochemistry.
- competitive and non-competitive assays radioimmunoassay, bioluminescence and chemiluminescence assays, fluorometric assay
- a radioimmunoassay (RIA) kit An angiostatin RIA is illustrated below. After successful radioiodination and purification of angiostatin or an angiostatin protein, the antiserum possessing the highest titer is added at several dilutions to tubes containing a relatively constant amount of radioactivity, such as 10,000 cpm, in a suitable buffer system. Other tubes contain buffer or preimmune serum to determine the non-specific binding.
- a dilution range (approximately 0.1 pg to 10 ng) of the angiostatin protein used for development of the antiserum is evaluated by adding known amounts of the protein to tubes containing radiolabeled protein and antiserum. After an additional incubation period, for example, 24 to 48 hours, protein A is added and the tubes centrifuged, supernatant removed and the radioactivity in the pellet counted. The displacement of the binding of radiolabeled angiostatin protein by the unlabeled angiostatin protein (standard) provides a standard curve. Several concentrations of other angiostatin protein fragments, plasminogen, angiostatin from different species, and homologous proteins are added to the assay tubes to characterize the specificity of the angiostatin antiserum.
- Extracts of various tissues including but not limited to primary and secondary tumors, Lewis lung carcinoma, cultures of angiostatin producing cells, placenta, uterus, and other tissues such as brain, liver, and intestine, are prepared using extraction techniques that have been successfully employed to extract angiostatin. After lyophilization or Speed Vac of the tisssue extracts, assay buffer is added and different aliquots are placed into the RIA tubes. Extracts of known angiostatin producing cells produce displacement curves that are parallel to the standard curve, whereas extracts of tissues that do not produce angiostatin do not displace radiolabeled angiostatin from the angiostatin antiserum.
- extracts of urine, plasma, and cerebrospinal fluid from animals with Lewis lung carcinoma are added to the assay tubes in increasing amounts.
- Parallel displacement curves indicate the utility of the angiostatin assay to measure angiostatin in tissues and body fluids.
- Tissue extracts that contain angiostatin are additionally characterized by subjecting aliquots to reverse phase HPLC. Eluate fractions are collected, dried in Speed Vac, reconstituted in RIA buffer and analyzed in the angiostatin RIA. The maximal amount of angiostatin immunoreactivity is located in the fractions corresponding to the elution position of angiostatin.
- the assay kit provides instructions, antiserum, angiostatin or angiostatin protein, and possibly radiolabeled angiostatin and/or reagents for precipitation of bound angiostatin-angiostatin antibody complexes.
- the kit is useful for the measurement of angiostatin in biological fluids and tissue extracts of animals and humans with and without tumors.
- This angiostatin immunohistochemistry kit provides instructions, angiostatin antiserum, and possibly blocking serum and secondary antiserum linked to a fluorescent molecule such as fluorescein isothiocyanate, or to some other reagent used to visualize the primary antiserum. Immunohistochemistry techniques are well known to those skilled in the art.
- This angiostatin immunohistochemistry kit permits localization of angiostatin in tissue sections and cultured cells using both light and electron microscopy. It is used for both research and clinical purposes. For example, tumors are biopsied or collected and tissue sections cut with a microtome to examine sites of angiostatin production.
- Another method to visualize sites of angiostatin biosynthesis involves radiolabeling nucleic acids for use in in situ hybridization to probe for angiostatin messenger RNA.
- the angiostatin receptor can be localized, visualized and quantitated with immunohistochemistry techniques.
- a Lewis lung carcinoma was selected in which the primary tumor most efficiently inhibited lung metastasis.
- Syngeneic C57BI6/J six-week-old male mice were injected (subcutaneous dorsum) with 1 x 10 6 tumor cells. Visible tumors first appeared after 3-4 days. When tumors were approximately 1500 mm 3 in size, mice were randomized into two groups. The primary tumor was completely excised in the first group and left intact in the second group after a sham operation. Although tumors from 500 mm 3 to 3000 mm 3 inhibited growth of metastases, 1500 mm 3 was the largest primary tumor that could be safely resected with high survival and no local recurrence.
- mice After 21 days, all mice were sacrificed and autopsied. In mice with an intact primary tumor, there were four +2 visible metastases, compared to fifty +5 metastases in the mice in which the tumor had been removed ( p ⁇ 0.0001 ). These data were confirmed by lung weight, which correlates closely with tumor burden, as has been previously demonstrated. There was a 400% increase in wet lung weight in the mice that had their tumors removed compared to mice in which the tumor remained intact ( p ⁇ 0.0001 ).
- This experimental model gave reproducible data and the experiment described is reproducible.
- This tumor is labeled "Lewis lung carcinoma - low metastatic" (LLC-Low).
- LLC-Low Lewis lung carcinoma - low metastatic
- the tumor also suppressed metastases in a nearly identical pattern in SCID mice, which are deficient in both B and T lymphocytes.
- a highly metastatic variant of Lewis lung carcinoma arose spontaneously from the LLC-Low cell line of Example 1 in one group of mice and has been isolated according to the methods described in Example 1 and repeatedly transplanted.
- This tumor (LLC-High) forms more than 30 visible lung metastases whether or not the primary tumor is present.
- Replication rate of tumor cells within metastases was determined by counting nuclei stained with BrdU which had been previously injected into the mice.
- the high percentage of tumor cells incorporating BrdU in small, avascular metastases of animals with an intact primary tumor was equivalent to the BrdU incorporation of tumor cells in the large vascularized metastases of mice from which the primary tumor had been removed ( Figure 3). This finding suggests that the presence of a primary tumor has no direct effect on the replication rate of tumor cells within a metastasis.
- the left panel shows BrdU labeling index of tumor cells in the lung in the presence or absence of a primary tumor.
- sections were permeabilized with 0.2 M HCI for 10 minutes and digested with 1 ⁇ g/ml proteinase K (Boehringer Mannheim GmbH, Mannheim, Germany) in 0.2 M Tris-HCl, 2 mM CaCt ⁇ at 37° C for 15 minutes. Labeling index was estimated by counting percentage of positive nuclei at 250 power.
- the right panel of Figure 3 depicts an analysis of total lung weight of tumors with primary tumors intact or removed 5, 10 and 15 days after operation. Animals were sacrificed 6 hours after intraperitoneal injection of BrdU (0.75 mg/mouse).
- the tumor was neovascularized.
- an intact primary tumor inhibits formation of new capillary blood vessels in metastases, but proliferation of tumor cells within a metastasis are not affected by the primary tumor.
- a primary tumor inhibits angiogenesis of a second tumor implanted in the mouse cornea. Growth of this second tumor is inhibited.
- a 0.25 to 0.5 mm 2 Lewis lung tumor (LLC-Low) was implanted in the mouse cornea on day 0. (Muthukkaruppan Vr., et al, Angiogenesis in the mouse cornea. Science 205: 1416-1418, 1979)
- a primary tumor was formed by inoculating 1 x 10 6 LLC-Low cells subcutaneously in the dorsum, either 4 or 7 days before the corneal implant; or on the day of the corneal implant; or 4 or 7 days after the corneal implant. Control mice received the corneal implant but not the subcutaneous tumor.
- control mice received the corneal implant and an inoculation of LLC-High tumor cells in the dorsum 4 days before the corneal implant.
- the corneas were evaluated daily by slit-lamp stereomicroscopy for the growth of the corneal tumor (measured by an ocular micrometer) and for the growth of new capillary vessels from the edge of the corneal limbus.
- a majority of corneas (6/8) developed neovascularization starting at day 6 to 7 days after corneal implantation and continuing to day 10.
- the vascularized corneal tumors had reached approximately a quarter of the volume of the whole eye.
- the corneal implants did not become vascularized if the primary tumor was in place by at least 4 days or more before the corneal implant (Table 1). In the absence of neovascularization, corneal tumors grew slowly as thin, white, avascular discs within the cornea.
- bFGF basic fibroblast growth factor
- Matrigel an extract of basement membrane proteins, containing either 25 or 50 ng/ml bFGF in the presence of heparin, was injected subcutaneously on the ventral surface of normal and tumor-bearing mice (LLC-Low). Mice were sacrificed 4 days later and hemoglobin concentration in the gel was measured to quantify blood vessel formation. It has previously been shown that the number of new vessels which enter the matrigel is correlated with hemoglobin concentration. (Folkman J., Angiogenesis and its inhibitors in "Important Advances in Oncology 1985". VT DeVita, S. Hellman and S. Rosenberg, editors, J.B. Lippincott, Philadelphia 1985) Some gels were also prepared for histological examination.
- matrigel pellets which contained 50 ng/ml bFGF were completely red. They were heavily invaded by new capillary vessels, and contained 2.4 g/dl hemoglobin. Matrigel which lacked bFGF was translucent and gray and contained only 0.4 g/dl hemoglobin (a 6-fold difference). In contrast, matrigel from mice with a primary tumor contained only 0.5 g/dl ( Figure 4).
- mice were implanted with Lewis lung carcinoma as described above. After 15 days, when tumors were approximately 1500 mm 3 , the mice were randomized into four groups. Three groups underwent complete surgical resection of the primary tumor; in one group the tumors were left in place (after a sham surgical procedure). The mice in the three resection groups then received daily intraperitoneal injections of saline, serum from normal nontumor bearing mice, or serum from mice with 1500 mm 3 Lewis lung carcinomas. The group of mice with the tumors left intact received intraperitoneal saline injections. All mice were treated for 21 days, after which the animals were euthanized and lung metastases were counted (Table 2).
- BCE cells are used between passages 9 and 14 only. At day 0, BCE cells are plated onto gelatinized (1.5 % gelatin in PBS at 37°, 10% C0 2 for 24 hours and then rinsed with 0.5 ml PBS) 24 well plates at a concentration of 12,500 cells/well.
- Cell counts are performed using a hemocytometer.
- Cells are plated in 500 ⁇ l DMEM with 10% heat-inactivated (56°C for 20 minutes) bovine calf serum and 1 % glutamine-pen-strep (GPS).
- BCE cells are challenged as follows: Media is removed and replaced with 250 ⁇ l of DMEM/5% BCS/1 %GPS. The sample to be tested is then added to wells. (The amount varies depending on the sample being tested) Plates are placed at 37°C/10% C0 2 for approximately 10 minutes. 250 ⁇ l of DMEM/5% BCS/1% GPS with 2ng/ml bFGF is added to each well. The final media is 500 ⁇ l of DMEM/5% BCS/1%GPS/ with 1 ng/ml bFGF. The plate is returned to 37°C/10% C0 2 incubator for 72 hours.
- a unit of activity is that amount of serum containing angiostatin that is capable of producing half- maximal inhibition of capillary endothelial proliferation when endothelial cells are incubated in bFGF 1 ng/ml for 72 hours.
- Bovine capillary endothelial cells were stimulated by basic fibroblast growth factor (bFGF 1 ng/ml), in a 72-hour proliferation assay.
- the serum of tumor-bearing mice added to these cultures inhibited endothelial cell proliferation in a dose-dependent and reversible manner. Normal serum was not inhibitory ( Figure 5).
- Endothelial cell proliferation was inhibited in a similar manner (relative to controls) by serum obtained from tumor-bearing nu/nu mice and SCID mice. After the primary tumor was removed, angiostatin activity disappeared from the serum by 3-5 days.
- Tumor-bearing serum also inhibited bovine aortic endothelial cells and endothelial cells derived from a spontaneous mouse hemangioendothelioma, (Obeso, et al., "Methods in Laboratory Investig ation, A Hemangioendothelioma-derived cell line; Its use as a Model for the Study of Endothelial Cell Biology," Lab Invest., 63(2), pgs 259-269, 1990) but did not inhibit Lewis lung tumor cells, 3T3 fibroblasts, aortic smooth muscle cells, mink lung epithelium, or W138 human fetal lung fibroblasts.
- Serum from mice bearing a primary tumor of the LLC- High did not significantly inhibit proliferation of bFGF- stimulated bovine capillary endothelial cells relative to controls. Also, when this serum was subjected to the first two steps of purification (heparin-Sepharose chromatography and gel filtration), angiostatin activity was not found in any fractions.
- mice received intraperitoneal injections of either LLC- Low or LLC-High tumor cells (10 6 ), and one week later, 1-2 ml of bloody ascites was obtained from each of 10-20 mice.
- LLC-High also appears to contain a predominance of endothelial cell stimulator, but angiostatin cannot be identified in the serum.
- angiostatin(s) serum was pooled from tumor-bearing mice.
- the inhibitory activity assayed according the above-described in vitro inhibitor activity assay, was sequentially chromatographed using heparin-Sepharose, Biogel A0.5mm agarose, and several cycles of C4-reverse phase high performance liquid chromatography (HPLC). SDS-PAGE of the HPLC fraction which contained endothelial inhibitory activity, revealed a discrete band of apparent reduced M r of 3 8 , 000 Daltons, which was purified approximately 1 million-fold (see Table 3) to a specific activity of approximately 2xl0 7 .
- a unit of activity is that amount of serum containing angiostatin that is capable of producing half-maximal inhibition of capillary endothelial proliferation when endothelial cells are incubated in bFGF 1 ng/ml for 72 hours.
- Figure 7 shows C4 reverse phase chromatography of partially purified serum or urine from tumor-bearing animals. All fractions were assayed on bovine capillary endothelial cells with bFGF in a 72-hour proliferation assay as described in
- Example IX A discrete peak of inhibition was seen in both cases eluting at 30 - 35 % acetonitrile in fraction 23.
- SDS- polyacrylamide gel electrophoresis of inhibitory fraction from the third cycle of C4 reverse phase chromatography of serum from tumor-bearing animals showed a single band at about 38,000 Daltons.
- Endothelial inhibition was assayed according to the procedure described in Example 9.
- Angiostatin was isolated on a Synchropak HPLC C4 column. (Synchrom, Inc.
- Plasminogen lysine binding site I was obtained from
- the preparation is purified human plasminogen after digestion with elastase. Lysine binding site
- I obtained in this manner is a population of proteins that contain, in aggregate, at least the first three triple-loop structures (numbers 1 through 3) in the plasmin A-chain (Kringle 1 +2+3).
- Plasminogen lysine binding site I (Sigma Chemical Company, St. Louis, MO) was resuspended in water and applied to a C4-reversed phase column that had been equilibrated with HPLC-grade water/0.1% TFA. The column was eluted with a gradient of water/0.1 % TFA to acetonitrile/0.1 % TFA and fractions were collected into polypropylene tubes. An aliquot of each was evaporated in a speed vac, resuspended with water, and applied to BCEs in a proliferation assay. This procedure was repeated two times for the inhibitory fractions using a similar gradient for elution.
- the inhibitory activity eluted at 30-35% acetonitrile in the final run of the C4 column.
- SDS-PAGE of the inhibitory fraction revealed 3 discrete bands of apparent reduced molecular mass of 40, 42.5, and 45 kd.
- SDS-PAGE under non-reducing conditions revealed three bands of molecular mass 30, 32.5, and 35 kd respectively.
- the product was resuspended in saline and applied to bovine capillary endothelial cells stimulated by 1 ng/ml basic fibroblast growth factor in a 72 hour assay. Protein extracted from each of the three bands inhibited the capillary endothelial cells.
- mice were implanted with Lewis lung carcinomas and underwent resections when the tumors were 1500-2000 mm 3 .
- mice On the day of operation, mice were randomized into 6 groups of 6 mice each. The mice received daily intraperitoneal injections with the three purified inhibitory fragments of human plasminogen, whole human plasminogen, urine from tumor-bearing animals, urine from normal mice, or saline. One group of tumor-bearing animals that had only a sham procedure was treated with saline injections. Immediately after removal of the primary tumor, the mice receive an intraperitoneal injection of 24 ⁇ g (1.2 mg/kg/day/mouse) of the inhibitory plasminogen fragments as a loading dose. They then receive a daily intraperitoneal injections of 12 ⁇ g of the inhibitory fragment (0.6 mg/kg/day/mouse) for the duration of the experiment.
- mice receive the same dose of the whole plasminogen molecule after tumor removal.
- the urine of normal or tumor bearing mice is filtered, dialyzed extensively, lyophilized, and then resuspended in sterile water to obtain a 250 fold concentration.
- the mice are given 0.8 ml of the dialyzed urine concentrate, either from tumor bearing mice or normal mice, in two intraperitoneal injections on the day of removal of the primary tumor as a loading dose. They then receive daily intraperitoneal injections of 0.4 ml of the dialyzed and concentrated urine for the course of the experiment. Treatments were continued for 13 days at which point all mice were sacrificed and autopsied.
- Figure 8 shows surface lung metastases after the 13 day treatment.
- Surface lung metastases refers to the number of metastases seen in the lungs of the mice at autopsy. A stereomicroscope was used to count the metastases.
- Figure 8 shows the mean number of surface lung metastases that was counted and the standard error of the mean.
- the group of mice with the primary tumor present showed no metastases.
- the mice in which the primary tumor was resected and were treated with saline showed extensive metastases.
- the mice treated with the human derived plasminogen fragment showed no metastases.
- the mice treated with whole plasminogen showed extensive metastases indicating that the whole plasminogen molecule has no endothelial inhibitory activity.
- Those mice treated with dialyzed and concentrated urine from tumor bearing mice showed no metastases. Mice treated with concentrated urine from normal mice showed extensive metastases.
- Figure 9 shows that the weight of the lung was measured, similar results were obtained (Figure 9).
- Amino acid sequence of murine and human angiostatin The amino acid sequence of angiostatin isolated from mouse urine and angiostatin isolated from the human lysine binding site I fragment preparation was determined on an Applied Biosystem Model 477A protein sequencer. Phenylthiohydantoin amino acid fractions were identified with an on-line ABI Model 120A HPLC. The amino acid sequence determined from the N-terminal sequence and the tryptic digests of the murine and human angiostatin indicate that the sequence of the angiostatin is similar to the sequence beginning at amino acid number 98 of murine plasminogen.
- the amino acid sequence of the angiostatin is a molecule comprising a protein having a molecular weight of between approximately 38 kilodaltons and 45 kilodaltons as determined by reducing polyacrylamide gel electrophoresis and having an amino acid sequence substantially similar to that of a murine plasminogen fragment beginning at amino acid number 98 of an intact murine plasminogen molecule.
- the beginning amino acid sequence of the murine angiostatin (SEQ ID NO: 2) is shown in Figure 1.
- the length of the amino acid sequence may be slightly longer or shorter than that shown in the Figure 1.
- N terminal amino acid analysis and tryptic digests of the active fraction of human lysine binding site I show that the sequence of the fraction begins at approximately amino acid 97 or 99 of human plasminogen and the human angiostatin is homologous with the murine angiostatin.
- the beginning amino acid sequence of the human angiostatin (starting at amino acid 98) is shown in Figure 2, (SEQ ID NO:3).
- the amino acid sequence of murine and human angiostatin is compared in Figure 2 to corresponding internal amino acid sequences from plasminogen of other species including porcine, bovine, and Rhesus monkey plasminogen, indicating the presence of angiostatin in those species.
- the pTrcHisA vector (Invitrogen) (Fig. 10) was used to obtain high-level, regulated transcription from the trc promoter for enhanced translation efficiency of eukaryotic genes in E. coli.
- Angiostatin is expressed fused to an N- terminal nickel-binding poly-histidine tail for one-step purification using metal affinity resins.
- the enterokinase cleavage recognition site in the fusion protein allows for subsequent removal of the N-terminal histidine fusion protein from the purified recombinant protein.
- the recombinant human angioststin protein was found to bind lysine; is cross- reactive with monoclonal antibodies specific for kringle regions 1, 2 and 3, and inhibits bFGF-driven endothelial cell proliferation in vitro.
- the gene fragment encoding human angiostatin is obtained from human liver mRNA which is reverse transcribed and amplified using the polymerase chain reaction (PCR) and specific primers.
- the product of 1131 base pairs encodes amino acids 93 to 470 of human plasminogen.
- the amplified fragment was cloned into the Xhol/Kpnl site of pTrcHisA, and the resultant construct transformed into XL- IB (available from Stratagene) E. coli host cells.
- a control clone containing the plasmid vector pTrcHisA alone was transformed inot XL- IB E. coli host cells as well. This clone is referred to as the vector control clone. Both clones were purified identically as described below.
- clone pTrcHisA/HAsH4 was selected. Induction with IPTG was for 4 hours after which the cell pellet was collected and resuspended in 50 mM Tris pH 8.0, 2 mM EDTA, 5% glycerol and 200 mg/ml lysozyme and stirred for 30 min. at 4°C. The slurry was centrifuged at 14,000 rpm for 25 min. and the pellet resuspended in 50 mM Tris pH 8.0, 2 mM EDTA, 5% glycerol and 0.1% DOC. This suspension was stirred for 1 hr. at 4°C, and then centrifuged at 14,000 rpm for 25 min.
- the supernatant fraction at this step contains expressed angiostatin.
- the E. coli expressed human angiostatin was found to possess the physical property of native angiostatin, that is the ability to bind lysine.
- the E. coli expressed angiostatin was thus purified over a lysine-sepharose (Pharmacia or Sigma) column in a single step. Elution of angiostatin from the column was with 0.2M epsilon-amino-n- caproic acid pH7.5. Subsequent to these experiments, scale-up 10 L fermentation batches of clone pTrcHisA/HAsH4 was performed.
- the cells obtained from this scaled-up induction were pelleted and resuspended in50 mM Tris pH7.5, cracked at 10,000 psi thrice chilling at 10 °C in-between passes.
- the lysate obtained was clarified by centrifugation at 10,000 rpm for 30 min at 4°C, and expressed angiostatin isolated over lysine-sepharose (Fig. 1 1).
- E. coli expressed human angiostatin was dialysed exhaustively against water and lyophilized.
- the expressed human angiostatin was resuspended in media (DMEM, 5% BCS, 1% Gentamycin/ penicillin/streptomycin) to an estimated concentration of 3 ug/ml, and used in bovine capillary endothelial (BCE) cell assays in vitro, as described in EXAMPLE 8, pg.39.
- BCE bovine capillary endothelial
- Percent inhibition was calculated as follows: number of cells with angiostatin - number of cells at day 0
- the percent inhibition of BCE cell proliferation was comparable or higher to that of plasminogen derived angiostatin at similar concentrations.
- the results from a repeat run of the BCE assay are depicted in Fig.1 3, where at a 1 :5 dilution of the stock, recombinant angiostatin gave similar percent inhibitions to those obtained with plasminogen derived angiostatin.
- Figure 13 shows the surprising result that human recombinant angiostatin protein inhibits over 60%, and as much as over 75% of BCh proliferation in culture.
- Angiostatin maintains dormancy of micrometastases by increasing the rate of apoptosis.
- mice Following subcutaneous inoculation of C57 BL6/J mice with Lewis lung carcinoma cells (1 xlO ), primary tumors of approximately 1.5 cm developed. Animals were subject to either surgical removal of the primary tumor or sham surgery. At 5, 10 and 15 days after surgery, mice were sacrificed and their lungs prepared for histological examination. Animals with resected primary tumors showed massive proliferation of micrometastases compared to sham operated controls (Fig. 14). These changes were accompanied by a significant increase in lung weight.
- TNP- 1470 O- chloroacetylcarbamoyl fumagillol, previously named AGM- 1470
- AGM- 1470 an analogue of fumagillin with reported anti- angiogenic activity.
- Subcutaneous injection of TNP-1470 (30 mg/kg every two days) produced results that were strikingly similar to those described above for animals that had intact primary tumors.
- These animals displayed a lower lung weight, equivalent proliferative index and increased apoptotic index compared to saline-injected controls (Fig. 16).
- Fig. 16 aline-injected controls
- Angiostatin was purified from human plasminogen by limited elastase digestion as described in Example 15 above.
- Angiostatin was resuspended in phosphate-buffered saline for administration into six week old male C57BI6/J mice. Animals were implanted subcutaneously with 1 X 10 tumor cells of either the Lewis lung carcinoma or T241 fibrosarcoma. Treatment with angiostatin is begun after four days when tumors are 80-160 mm in size. Mice received angiostatin injections in either a single injection of 40 mg/kg or two 80 mg/kg injections via intraperitoneal (ip) or subcutaneous (sc) routes. Animals were sacrificed at various times after treatment extending to 19 days.
- Angiostatin administered at a daily dose of 40 mg/kg ip, produced a highly significant inhibition of the growth of T241 primary tumors (Fig. 17). This inhibitory effect on growth was visibly evident within 2 days and increased in magnitude throughout the time course of the study. By day 18, angiostatin-treated mice had tumors that were approximately 38% of the volume of the saline injected controls. This difference was statistically significant (p ⁇ 0.001 , Students t-test). Angiostatin treatment (total dose of 80 mg/kg/day, administered twice daily at 40 mg/kg ip or sc) also significantly reduced the growth rate of LLC-LM primary tumors (Fig. 17). This inhibitory effect was evident at 4 days and increased in magnitude at all subsequent times examined. On the last day of the experiment (day 19), angiostatin-treated mice possessed a mean tumor volume that was only 20% of the saline-injected controls which was significantly different (p ⁇ 0.001 Students t-test).
- angiostatin was administered (50 mg/kg ql2h) to mice implanted with T241 fibrosarcoma, Lewis lung carcinoma (LM) or reticulum cell sarcoma cells.
- T241 fibrosarcoma the angiostatin treated mice had mean tumor volumes that were only 15% of the untreated mice at day 24.
- Figure 20 demonstrates that for Lewis lung carcinoma (LM), the angiostatin treated mice had mean tumor volumes that were only 13% of the untreated mice at day 24.
- Figure 21 demonstrates that for reticulum sacroma, the angiostatin treated mice had mean tumor volumes that were only 19% of the untreated mice at day 24.
- the data represent the average of 4 mice at each time point.
- angiostatin is an extremely potent inhibitor of the growth of three different primary tumors in vivo.
- angiostatin on two human tumor cell lines, human prostate carcinoma PC-3 and human breast carcinoma MDA-MB.
- Immunodeficient SCID mice were implanted with human tumor cells, and the mice treated with 50 mg/kg angiostatin every 12 hours essentially as described in
- Example 21 The results demonstrate that the angiostatin protein of the present invention is a potent inhibitor of human tumor cell growth.
- Figure 22 shows that for human prostate carcinoma PC-3, the angiostatin treated mice had only 2% of the mean tumor volume compared to the untreated control mice at day 24.
- Figure 23 shows that for human breast carcinoma MDA-MB, he angiostatin treated mice had only 8% of the mean tumor volume compared to the untreated control mice at day 24.
- a 1380 base pair DNA sequence for angiostatin derived from mouse plasminogen cDNA obtained from American Type Culture Collection (ATCC)
- ATCC American Type Culture Collection
- mouse plasminogen amino acids 1 - 460 was generated using PCR and inserted into an expression vector.
- the expression vector was transfected into T241 fibrosarcoma cells and the transfected cells were implanted into mice. Control mice received either non-transfected T241 cells, or T241 cells transfected with the vector only (i.e. non-angiostatin expressing transfected cells). Three angiostatin-expressing transfected cell clones were used in the experiment. Mean tumor volume determined over time.
- mice show the surprising and dramatic reduction in mean tumor volume in mice for the angiostatin-expressing cells clones as compared with the non-transfected and non-expressing control cells.
- the mouse DNA sequence coding for mouse angiostatin protein is derived from mouse plasminogen cDNA.
- the mouse angiostatin encompasses mouse plasminogen kringle regions 1-4.
- the schematic for constructing this clone is shown in Figure 24.
- the mouse angiostatin protein clones were transfected into T241 fibrosarcoma cells using the LIPOFECTINTM transfection system (available from Life Technologies, Gaithersburg, MD).
- the LIPOFECTINTM reagent is a 1 : 1 (w/w) liposome formulation of the cationic lipid N-[l-(2,3- dioleyloxy) ⁇ ropyl]-n,n,n-trimethylammonium chloride
- DOTMA diolecoyl phosphotidylethanolamine
- DOPE diolecoyl phosphotidylethanolamine
- T241 cells are grown in 60 cm 2 tissue culture dishes, seed -1 -2 x 10 5 cells in 2 ml of the appropriate growth medium supplemented with serum.
- Solution A For each transfection, dilute 5 ⁇ g of DNA in 100 ⁇ l of serum-free OPTI-MEM I Reduced Serum Medium (available from Life Technologies) (tissue culture grade deionized water can also be used).
- Solution B For each transfection, dilute 30 ⁇ g of LIPOFECTIN in 100 ⁇ l OPTI-MEM medium.
- Transfected cells can be assayed for expression of angiostatin protein using angiostatin-specific antibodies.
- G418 resistant colonies appeared in the CMV-angiostatin transfected T241 cells. Also, a number of clones were seen in the vector alone transfected clones but not in the untransfected clones. The G418 resistant clones were selected for their expression of angiostatin, using a immunofluorence method.
- Figure 27 depicts the results of the transfection experiment. All three of the angiostatin-expressing T241 transfected clones produced mean tumor volumes in mice that were substantially reduced relative to the tumor volume in contol mice. The mean tumor volume of the mice implanted with Clone 37 was only 13% of the control, while Clone 31 and Clone 25 tumor volumes were only 21% and 34% of the control tumor volumes, respectively. These results demonstrate that the DNA sequences coding for angiostatin can be transfected into cells, that the transfected DNA sequences are capable of expressing angiostatin protein by implanted cells, and that the expressed angiostatin fucntions in vivo to reduce tumor growth.
- RNA from various cell types Lewis lung carcinoma cells (mouse), T241 fibrosarcoma (mouse), and Burkitt's lymphoma cells (human), both from fresh tumor or cell culture after several passages were analysed to determine the presence of angiostatin transcripts.
- Northern analysis of samples showed an absence of any signal hybridizing with thn sequence from all samples except that of normal mouse liver
- RNA showing a single signal of approximately 2.4 kb corresponding to mouse plasminogen Northern analysis of human samles show an absence of any signal hybridizing with human angiostatin sequence from all samples except that of normal human liver RNA showing a single signal of approximately 2.4 kb corresponding to human plasminogen.
- RT-PCR Reverse transcription polymerase chain reaction
- human angiostatin transcripts (assuming identity with amino acids 93 to 470 of human plasminogen) are not produced by the above human samples.
- the positive signals obtained in normal mouse/human liver is from hybridization with plasminogen.
- the gene fragment encoding amino acids 93 to 470 of human plasminogen was cloned into the XhoI/EcoRI site of pHIL-SI(Invitrogen) which allows the secreted expression of proteins using the PHOl secretion signal in the yeast Pichia pastoris.
- the gene fragment encoding amino acids 93 to 470 of human plasminogen was cloned into the SnaBI/EcoRI site of pPIC9 (Invitrogen) which allows the secreted expression of proteins using the a-factor secretion signal in the yeast Pichia pastoris.
- the expressed human angiostatin proteins in these systems will have many advantages over those expressed in E. coli such as protein processing, protein folding and posttranslational modification inclusive of glycosylation. Expression of gene in P. pastoris: is described in )
- Transgenic animals such as of the bovine or procine family are created which express the angiostatin gene transcript.
- the transgenic animal express angiostatin protein for example in the milk of these animals.
- Additionally edible transgenic plants which express the angiostatin gene transcript are constructed.
- angiostatin fragment means a protein derivative of angiostain, or plasminogen, having an endothelial cell proliferation inhibiting activity.
- Angiostatin fragments are useful for treating angiogenic-mediated diseases or conditions.
- angiostatin fragments can be used to inhibit or suppress tumor growth.
- amino acid sequence of such an angiostatin fragment for example, can be selected from a portion of murine plasminogen (SEQ ID NO: l), murine angiostatin (SEQ ID NO:2); human angiostatin (SEQ ID NO:3), Rhesus angiostatin (SEQ ID NO:4), porcine angiostatin (SEQ ID NO:5), and bovine angiostatin (SEQ ID NO:6), unless indicated otherwise by the context in which it is used.
- murine plasminogen SEQ ID NO: l
- murine angiostatin SEQ ID NO:2
- human angiostatin SEQ ID NO:3
- Rhesus angiostatin SEQ ID NO:4
- porcine angiostatin SEQ ID NO:5
- bovine angiostatin SBEQ ID NO:6
- kringle 1 means a protein derivative of plasminogen having an endothelial cell inhibiting activity or anti-angiogenic activity, and having an amino acid sequence comprising a sequence homologous to kringle 1, exemplified by, but not limited to that of murine kringle 1 (SEQ ID NO:7), human kringle 1 (SEQ ID NO:8), Rhesus kringle 1 (SEQ ID NO:9), porcine kringle 1 (SEQ ID NO: 10), and bovine kringle 1 (SEQ ID NO: 11), unless indicated otherwise by the context in which it is used.
- murine kringle 1 SEQ ID NO:7
- human kringle 1 SEQ ID NO:8
- Rhesus kringle 1 SEQ ID NO:9
- porcine kringle 1 SEQ ID NO: 10
- bovine kringle 1 SEQ ID NO: 11
- Murine kringle 1 corresponds to amino acid positions 103 to 181 (inclusive) of murine plasminogen of SEQ ID NO: l , and corresponds to amino acid positions 6 to 84 (inclusive) of murine angiostatin of SEQ ID NO:2.
- Human kringle 1 (SEQ ID NO: 8), Rhesus kringle 1 (SEQ ID NO:9), porcine kringle 1 (SEQ ID NO: 10), and bovine kringle 1 (SEQ ID NO: 1 1 ) correspond to amino acid positions 6 to 84 (inclusive) of angiostatin of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6, respectively.
- kringle 2 means a protein derivative of plasminogen having an endothelial cell inhibiting activity or anti-angiogenic activity, and having an amino acid sequence comprising a sequence homologous to kringle 2, exemplified by, but not limited to that of murine kringle 2 (SEQ ID NO: 12), human kringle 2 (SEQ ID NO: 13), Rhesus kringle 2 (SEQ ID NO: 14), porcine kringle 2 (SEQ ID NO: 15), and bovine kringle 2 (SEQ ID NO: 16), unless indicated otherwise by the context in which it is used.
- murine kringle 2 SEQ ID NO: 12
- human kringle 2 SEQ ID NO: 13
- Rhesus kringle 2 SEQ ID NO: 14
- porcine kringle 2 SEQ ID NO: 15
- bovine kringle 2 SEQ ID NO: 16
- Murine kringle 2 corresponds to amino acid positions 185 to 262 (inclusive) of murine plasminogen of SEQ ID NO: l, and corresponds to amino acid positions 88 to 165 (inclusive) of murine angiostatin of SEQ ID NO:2.
- Human kringle 2 (SEQ ID NO: 13), Rhesus kringle 2 (SEQ ID NO: 14
- porcine kringle 2 (SEQ ID NO: 15), and bovine kringle 2 (SEQ ID NO: 16) correspond to amino acid positions 88 to 165 (inclusive) of angiostatin of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6, respectively.
- kringle 3 means a protein derivative of plasminogen having an endothelial cell inhibiting activity or anti-angiogenic activity, and having an amino acid sequence comprising a sequence homologous to kringle 3, exemplified by, but not limited to that of murine kringle 3 (SEQ ID NO: 17), human kringle 3 (SEQ ID NO: 18), Rhesus kringle 3
- Murine kringle 3 corresponds to amino acid positions 275 to 352 (inclusive) of murine plasminogen of SEQ ID NO: l, and corresponds to amino acid positions 178 to 255 (inclusive) of murine angiostatin of SEQ ID NO:2.
- Human kringle 3 (SEQ ID NO: 18), Rhesus kringle 3 (SEQ ID NO: 19), porcine kringle 3 (SEQ ID NO:20), and bovine kringle 3 (SEQ ID NO:21) correspond to amino acid positions 178 to 255 (inclusive) of angiostatin of SEQ ID NO: 3, SEQ ID NO:4,
- kringle 4" means a protein derivative of plasminogen having an endothelial cell inhibiting activity or anti-angiogenic activity, and having an amino acid sequence comprising a sequence homologous to kringle 4, exemplified by, but not limited to that of murine kringle 4 (SEQ ID NO:22) and human kringle 4 (SEQ ID NO:23), unless indicated otherwise by the context in which it is used.
- Murine kringle 4 corresponds to amino acid positions 377 to 454 (inclusive) of murine plasminogen of
- kringle 2-3 means a protein derivative of plasminogen having an endothelial cell inhibiting activity or anti-angiogenic activity, and having an amino acid sequence comprising a sequence homologous to kringle 2-3, exemplified by, but not limited to that of murine kringle 2-3 (SEQ ID NO:24), human kringle 2-3 (SEQ ID NO:25), Rhesus kringle 2-3 (SEQ ID NO:26), porcine kringle 2-3 (SEQ ID NO:27), and bovine kringle 2-3 (SEQ ID NO:28), unless indicated otherwise by the context in which it is used.
- Murine kringle 2-3 Murine kringle 2-3
- SEQ ID NO:24 corresponds to amino acid positions 185 to 352 (inclusive) of murine plasminogen of SEQ ID NO:l, and corresponds to amino acid positions 88 to 255 (inclusive) of murine angiostatin of SEQ ID NO:2.
- Human kringle 2-3 (SEQ ID NO:25), Rhesus kringle 2-3 (SEQ ID NO:26), porcine kringle 2-3 (SEQ ID NO:27), and bovine kringle 2-3 (SEQ ID NO:28) correspond to amino acid positions 88 to 255 (inclusive) of angiostatin of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6, respectively.
- kringle 1-3 means a protein derivative of plasminogen having an endothelial cell inhibiting activity or anti-angiogenic activity, and having an amino acid sequence comprising a sequence homologous to kringle 1-3, exemplified by, but not limited to that of murine kringle 1-3 (SEQ ID NO: 1
- Murine kringle 1-3 corresponds to amino acid positions 103 to 352 (inclusive) of murine plasminogen of SEQ ID NO: l, and corresponds to amino acid positions 6 to 255 (inclusive) of murine angiostatin of SEQ ID NO:2.
- Human kringle 1-3 (SEQ ID NO:30), Rhesus kringle 1-3 (SEQ ID NO:31), porcine kringle 1-3 (SEQ ID NO:32), and bovine kringle 1-3
- SEQ ID NO:33 correspond to amino acid positions 6 to 255 (inclusive) of angiostatin of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6, respectively.
- kringle 1-2 means a protein derivative of plasminogen having an endothelial cell inhibiting activity or anti-angiogenic activity, and having an amino acid sequence comprising a sequence homologous to kringle 1-2, exemplified by, but not limited to that of murine kringle 1-2 (SEQ ID NO:34), human kringle 1-2 (SEQ ID NO:35), Rhesus kringle 1-2 (SEQ ID NO:36), porcine kringle 1-2 (SEQ ID NO:37), and bovine kringle 1-2 (SEQ ID NO:38), unless indicated otherwise by the context in which it is used.
- Murine kringle 1-2 corresponds to amino acid positions 103 to 262 (inclusive) of murine plasminogen of SEQ ID NO: l, and corresponds to amino acid positions 6 to 165 (inclusive) of murine angiostatin of SEQ ID NO:2.
- Human kringle 1-2 (SEQ ID NO:35), Rhesus kringle 1-2 (SEQ ID NO:36), porcine kringle 1-2 (SEQ ID NO:37), and bovine kringle 1-2 (SEQ ID NO: 38) correspond to amino acid positions 6 to 165 (inclusive) of angiostatin of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6, respectively.
- kringle 1-4 means a protein derivative of plasminogen having an endothelial cell inhibiting activity or anti-angiogenic activity, and having an amino acid sequence comprising a sequence homologous to kringle 1-4, exemplified by, but not limited to that of murine kringle 1-4 (SEQ ID NO:39) and human kringle 1-4 (SEQ ID NO:40), unless indicated otherwise by the context in which it is used.
- Murine kringle 1-4 corresponds to amino acid positions 103 to 454 (inclusive) of murine plasminogen of SEQ ID NO: 1.
- Kringle 1, kringle 2, kringle 3, kringle 4, kringle 2-3, kringle 1-3, kringle 1-2 and kringle 1-4 amino acid sequences are respectively homologous to the specific kringle sequences identified above.
- the amino acid sequences have a degree of homology to the disclosed sequences of at least 60%, more preferably at least 70%, and more preferably at least 80%. It should be understood that a variety of amino acid substitutions, additions, deletions or other modifications to the above listed fragments may be made to improve or modify the endothelial cell proliferation inhibiting activity or anti- angiogenic activity of the angiostatin fragments. Such modifications are not intended to exceed the scope and spirit of the claims.
- cysteine residues C4 in recombinant human kringle 2 (SEQ ID NO: 13) and C42 in recombinant kringle 3 (SEQ ID NO: 18) were mutated to serines.
- amino acid substitutions, additions, deletions or other modifications can be made in the above identified angiostatin fragments, which do not significantly alter the fragments' endothelial cell proliferation inhibiting activity, and which are, therefore, not intended to exceed the scope of the claims.
- angiostatin fragment has at least 60%, more preferably at least 70%, and more preferably at least 80% of the endothelial cell proliferation inhibiting activity compared to that of the closest homologous angiostatin fragment disclosed herein.
- PCR-based method was used to generate the cDNA fragments coding for kringle 1 (Kl), kringle 2 (K2), kringle 3 (K3), kringle 4 (K4) and kringle 2-3 (K2-3) of human plasminogen (HPg).
- K 1-3, Kl-4 and K4 were prepared by digestion of Lys-HPg (Abbott Labs) with porcine elastase (Sigma) as previously described (Powell, J.R., and Castellino, F.J. (1983) Biochem. 22, 923-927). Briefly, 1.5 mg elastase was incubated at room temperature with 200 mg of human plasminogen in 50 mM Tris-HCl pH 8.0 overnight with shaking. The reaction was terminated by the addition of diisopropyl fluorophosphate (DFP) (Sigma) to a final concentration of ImM. The mixture was rocked for an additional 30 minutes at room temperature and dialyzed overnight against 50 mM Tris-HCl, pH 8.0.
- DFP diisopropyl fluorophosphate
- Recombinant Kl was expressed in DH5 ⁇ E. coli bacterial cells using a pSTII plasmid vector. This protein was purified to homogeneity by chromatography using lysine-
- E. coli bacterial cells (strain HB 101 ) expressing rK2 and rK3 were grown to an OD600 of approximately 0.8 at 3 °C in 2 x YT medium containing 100 mg/ml ampicillin and 25 mg/ml kanamycin.
- IPTG isopropyl-b-D-thiogalactopyranoside
- the cells were harvested by centrifugation and the pellets were stored at -80°C. The thawed cell lysates were re-suspended in the extraction buffer
- the proteolytically cleaved fragments of Kl-3, Kl-4 and K4 were purified using a lysine-Sepharose 4B column (2.5 cm x 15 cm) equilibrated with 50 mM Tris-HCl, pH 8.0 until an absorbance at 180 nm reached 0.005.
- the absorbed kringle fragments were eluted with Tris buffer containing 200 mM ⁇ - aminocaproic acid, pH 8.0.
- the eluted samples were the dialyzed overnight against 20 mM Tris-HCl, pH 5.0, and were applied to a BioRad Mono-S column equilibrated with the same buffer.
- K4, Kl-3 and Kl-4 were eluted with 0-20%, " 20-50% and 50-70% step-gradients of 20 mM phosphate/1 M KC1 , pH 5.0. Most Kl-3 and Kl-4 fragments were eluted from the column with 0.5 M KC1 as determined by SDS-PAGE. All fractions were dialyzed overnight against 20 mM Tris-HCl, pH 8.0. After dialysis, Kl-3 and Kl-4 fragments were further purified using a heparin-Sepharose column (5 cm x 10 cm) (Sigma) pre- equilibrated with 20 mM Tris-HCl buffer, pH 8.0.
- the Kl-3 fragment was eluted with 350 mM KC 1 and Kl-4 was recovered from the flow-through fraction.
- the purified kringle fragments were analyzed on SDS-gels follows by silver-staining, by Western immunoblotting analysis with anti- human K4 and Kl-3 polyclonal antibodies, and by amino- terminal sequencing analysis.
- oxidized glutathione was added to a final concentration of 1.25 mM and incubated for 6 hours at 4°C.
- the renatured protein was dialyzed initially against H2O for 2 days and for an additional two days against 50 mM phosphate-buffered saline, pH 8.0.
- the solution was then loaded onto a lysine-Bio-Gel column (2 cm x 13 cm) equilibrated with the same phosphate-buffered saline.
- the column was washed with phosphate-buffered saline and protein was eluted with a phosphate buffer containing 50 mM 6- AH A (6-aminohexanoic acid).
- Reverse-phase HPLC was performed on an Aquapore Butyl column (2.1 x 100 mm, widepore 30 nm, 7 mm, Applied Biosystems) and a Hewlett Packard liquid chromatography was used with acetonitrile gradients.
- Bovine capillary endothelial (BCE) cells were isolated as previously described (Folkman, J., Haudenschild, CC, and Zetter, B.R. (1979) Proc. Natl. Acad. Sci USA. 76, 5217-
- the medium was replaced with 500 ml of fresh DMEM containing 5% BCS and samples of individual or combinatorial kringle fragments in triplicates were added to each well. After 30 minutes of incubation, bFGF was added to a final concentration of 1 ng/ml. After 72 hours of incubation, cells were trypsinized, re-suspended in Hematall (Fisher Scientific, Pittsburg, PA) and counted with a Coulter counter.
- Kl migrating with a higher molecular weight of 17 kDa was identified by SDS-gel electrophoresis.
- the fragments of Kl-4 and Kl-3 were obtained by proteolytic digestion of human Lys-plasminogen (Lys-HPg) with elastase as previously described (Powell, J.R., and Castellino, F.J.
- N-terminal sequence for K4 produced -VVQD- with approximately 20% -VQD-, followed by SEQ ID NO:23, each of which is predicted from the expected sequence beginning with Valine 1 ⁇ and Valinel77 0 f human angiostatin (SEQ ID NO:
- rKl inhibited BCE cell proliferation in a dose-dependent fashion.
- the concentration of rKl required to reach 50% inhibition (ED50) was about 320 nM (Table 4).
- ED50 50% inhibition
- rK4 exhibited little or no inhibitory effect on endothelial cell proliferation.
- Recombinant K2 and rK3, two non-lysine binding kringle fragments also produced a dose- dependent inhibition of endothelial cell proliferation (Fig. 30B).
- Fig. 34A the DTT-treated protein migrated at a higher position with molecular weight of about 42 kDa (lane 2) as compared to the native angiostatin with molecular weight of 33 kDa (lane 1), suggesting that angiostatin was completely reduced.
- the anti-proliferation activity of angiostatin was largely abolished after reduction (Fig. 34B). From these results, we conclude that the correct folding of angiostatin through the intra-kringle disulfide bonds is preferable to maintain its potent effect on inhibition of endothelial cell proliferation.
- K4 contains two positively charged lysine residues adjacent to cysteines 22 and
- angiostatin may interact with an endothelial cell adhesion receptor such as integrin a v b3, blocking integrin-mediated angiogenesis (Brooks, P.C., Montgomery, A.M., Rosenfeld, M., Reisfeld R.A., Hu, T.
- bFGF- and VEGF-induced angiogenesis were inhibited by angiostatin, it may block a common pathway for these integrin-mediated angiogenesis.
- D'Angelo et. al. reported that the antiangiogenic 16 kDa N- terminal fragment inhibited the activation of mitogen-activated protein kinase (MAPK) by VEGF and bFGF in capillary endothelial cells (D'Angelo, G., Struman, I., Martial, J., and Weiner, R. (1995) Proc. Natl. Acad. Sci. 92, 6374-6378).
- MAPK mitogen-activated protein kinase
- PF-4 Platelet factor 4 inhibits angiogenesis at high concentrations (Maione, T.E., Gray, G.S., Petro, A. J., Hunt, A.L., and Donner, S.I. (1990) Science 247,
- protease(s) may play a critical role in the regulation of angiogenesis. To date, little is known about the regulation of these protease activities in vivo.
- the data show that the disulfide bond mediated folding of the kringle structures in angiostatin is preferable to maintain its inhibitory activity on endothelial cell growth.
- Kringle structures analogous to those in plasminogen are also found in a variety of other proteins.
- apolipoprotein (a) has as many as 37 repeats of plasminogen kringle 4 (McLean, J.W., Tomlinson, J.E., Kuang, W.-J., Eaton, D.L., Chen, E.Y., Fless, G.M., Scanu, A.M., and
- prothrombin also contains two kringles that are homologous to those of plasminogen (Walz, D.A., Hewett-Emmett, D., and Seegers, W.H. (1977) Proc. Natl. Acad. Sci. 74, 1969-1973).
- Urokinase has been shown to possess a kringle structure that shares extensive homology with plasminogen (Gunzler, W.A., J., S.G., Otting, F., Kim, S.-M. A., Frankus, E., and Flohe, L. (1982) Hoppe-Seyler's A.
- surfactant protein B and hepatocyte growth factor (HGF) also carry kringle structures (Johansson, J., Curstedt, T., and Jornvall., H. (1991) Biochem. 30, 6917-6921 ; Lukker, N.A., Presta, L.G., and Godowski, P.J. (1994) Prot. Engin. 7, 895-903).
- the following example characterizes the activity of additional angiostatin fragments.
- the data suggests that potent anti-endothelial and tumor suppressive activity can be obtained from such protein fragments of angiostatin.
- kringle 1-4BKLS means a protein derivative of plasminogen having an endothelial cell inhibiting activity, and having an amino acid sequence comprising a sequence homologous to kringle 1-4BKLS, exemplified by, but not limited to that of murine kringle 1-4BKLS (SEQ ID NO:41), and human kringle 1-4BKLS (SEQ ID NO:42), unless indicated otherwise by the context in which it is used.
- Murine kringle 1-4BKLS (SEQ ID NO:41) corresponds to amino acid positions 93 to 470 (inclusive) of murine plasminogen of SEQ ID NO: l .
- an "angiostatin fragment” can be a plasminogen fragment and encompass an amino acid sequence larger than the angiostatin presented in SEQ ID NO: 3, for example, and still have therapeutic endothelial cell proliferation inhibiting activity or antiangiogenic activity.
- a kringle 1-4BLKS amino acid sequence is homologous to the specific kringle 1-4BLKS sequences identified above.
- the amino acid sequences have a degree of homology to the disclosed sequences of at least 60%, more preferably at least 70%, and more preferably at least 80%. It should be understood that a variety of amino acid substitutions, deletions and other modifications to the above listed fragments may be made to improve or modify the endothelial cell inhibiting activity of the fragments. Such modifications are not intended to exceed the scope and spirit of the claims.
- This plasmid contained sequences encoding amino acids 93 to 470 of human plasminogen (SEQ ID NO:42) for cloning into the Xho I/ECo RI site of pHIL-S 1 expression vector using the P. pastoris native secretion signal PHO 1.
- angiostatin from pHIL-S l/HAsl 8 was typical for a His+ Mut s clone.
- 1L of OD600 cells were cultured in 150 ml of buffered metanol complex medium containing 1 % yeast extract, 2% peptone, 100 mM potassium phosphate pH 6.0, 1.34% yeast nitrogen base with ammonium sulfate, 0.00004% biotin and 0.5% methanol, in a 1L baffled flask.
- Cells were constantly shaken at 30°C, 250 rpm.
- Methanol was batch fed at 24 hour intervals by addition of absolute methanol to a final of 0.5%. After 120 hours cells were spun at 5,000 rpm for 10 minutes, and supernatants were stored at -70°C until used.
- 60 g lysine-sepharose 4B (Pharmacia) was resuspended in 500 ml ice-cold 50 mM phosphate buffer, pH 7.5 and used to pack a 48 x 100 mm column (-180 ml packed volume). The column was washed overnight with 7.5 column volumes (CV) of 50 mM sodium phosphate buffer, pH 7.5, at a flow rate of 1.5 ml/min. The sample was pumped onto the column at a flow rate of 1.5 ml/min and the column washed with 1.5 CV of 50 mM sodium phosphate, pH 7.5, at a flow rate of 3 ml/min.
- CV column volumes
- Bovine Capillary Endothelial Cell Proliferation Assay Bovine capillary endothelial Cell Proliferation Assay Bovine capillary endothelial cells were obtained as previously described. The cells are maintained in DMEM containing 3 mg/ml of recombinant human bFGF (Scios Nova, Mountainview, CA), supplemented with 10% heat-inactivated bovine calf serum, 100 U/ml penicillin, 100 mg/ml streptomycin, and 0.25 mg/ml fungizone (BioWhittaker) in 75 cm 2 cell-culture flasks. The assay was performed as described previously.
- DMEM containing 3 mg/ml of recombinant human bFGF (Scios Nova, Mountainview, CA), supplemented with 10% heat-inactivated bovine calf serum, 100 U/ml penicillin, 100 mg/ml streptomycin, and 0.25 mg/ml fungizone (BioWhittaker) in 75 cm 2 cell-culture flas
- LLC-LM murine Lewis lung carcinoma-low metastatic
- FIG. 36A and B Expressed angiostatin from P. pastoris as well as angiostatin purified by elastase cleavage of plasminogen were recognized by a conformationally dependent monoclonal antibody against kringle 1 to 3 (Castellino, Enzyme Research Laboratories, Inc., South Bend, IN) (Fig. 36B). This antibody fails to recognize reduced forms of plasminogen or angiostatin.
- P. pastoris expressed angiostatin is seen as a doublet that migrates at 49 kDa and 51.5 kDa on denatured unreduced SDS-PAGE Coomassie stained gels.
- P. pastoris expressed proteins are post-translationally modified with the majority of
- BCEs were cultured in the presence of bFGF to determine if the addition of purified recombinant angiostatin would inhibit the proliferation of BCEs.
- Purified P. pastoris-Qxpvessed angiostatin inhibited the bFGF-driven proliferation of bovine endothelial cells in vitro (Fig. 38B) in a dose dependent manner (Fig. 38C).
- Fig. 38B Purified P. pastoris-Qxpvessed angiostatin inhibited the bFGF-driven proliferation of bovine endothelial cells in vitro
- Fig. 38C a dose dependent manner
- At 1 ug/ml of recombinant angiostatin inhibition was 80%. The 50% inhibition was equivalent to that obtained with angiostatin derived from elastase cleavage of human plasminogen.
- the transplantable murine LLC (LM) line from which angiostatin was first identified was used. When implanted subcutaneously in syngenic C57B1/6J mice, these tumors grow rapidly, producing > 1.5 cm ⁇ tumors within 14 days. Following primary tumor resection, the micometastases in the lungs grow exponentially, to completely cover the surface of the lung. These metastases are highly vascularized by day 14 after primary tumor resection. If the primary tumor is left on, the micrometastases remain dormant and are not macroscopically visible. Recombinant angiostatin was administered systemically to mice following primary tumor resection to test the suppression of the growth of metastases. P.
- mice that had primary tumors resected and that received daily doses of recombinant angiostatin or angiostatin obtained from elastase cleavage of plasminogen were of comparable to those of normal mice (190 to 200 mg).
- Lungs of mice that had their primary tumors resected and subsequently treated with daily doses of recombinant angiostatin were pink with minimal numbers of unvascularized micrometastases (Fig. 40).
- Angiostatin protein expressed by P. pastoris possesses two important physical characteristics of the natural protein: ( 1 ) it is recognized by a conformationally dependent monoclonal antibody raised against kringle 1 to 3 of human plasminogen (Fig. 36B) and (2) it binds lysine (Fig. 36A and B). These properties indicated that the recombinant angiostatin protein was expressed with a conformation that mimics the native molecule. P.
- angiostatin protein inhibits the proliferation of bovine capillary endothelial cells stimulated by bFGF in vitro (fig. 38). when administered systemically, the recombinant angiostatin maintained the otherwise lethal metastastic Lewis lung carcinoma in a suppressed state (Fig. 39 A and B and Fig. 40).
- Preliminary data shows the absence of a detectable transcript for angiostatin in Lewis lung tumors freshly resected from mice or in LLC cells after 4 passages in in vitro culture. Plasminogen, produced by the liver, is maintained in circulation at a stable plasma concentration of 1.6 + 0.2 ⁇ M. It is possible that LLC-LM tumors produce an enzyme that cleaves plasminogen, bound or in circulation, to produce angiostatin. Alternatively inflammatory cells attracted to the tumor site could produce such an enzyme.
- Supernatants contain 100 mg/L of the protein. Therefore, the quantities required for clinical trials should be straightforward to produce and purify using standard technology well-known to those skilled in the art.
- the development of this expression system, and the demonstration of the in vitro and in vivo activity of purified recombinant angiostatin against metastases provided the foundation for assessment of the capacity of these fragments to inhibit tumor growth and prolong life in cancer patients and others suffering from angiogenic-mediated disease.
- Kringle 1-5 Angiostatin Protein Fragment The following example describes one method for the production of kringle 1-5 angiostatin protein fragment.
- kringle 1-5" means a protein derivative of plasminogen having an endothelial cell inhibiting activity or anti-angiogenic activity, and having an amino acid sequence comprising a sequence homologous to kringle 1-5, exemplified by, but not limited to that of murine kringle 1 -5 corresponding to amino acid positions 102 to 560 (inclusive) of murine plasminogen of SEQ ID NO: l .
- Kringle 5 itself is represented in the murine sequence of plasminogen of SEQ ID NO: l at amino acid positions 481-560 (inclusive).
- Kringle 1-5 amino acid sequences are respectively homologous to the specific kringle 1-5 sequence identified above.
- the amino acid sequences have a degree of homology to the disclosed sequences of at least 60%, more preferably at least 70%, and more preferably at least 80%.
- a variety of amino acid substitutions, additions, deletions or other modifications to the above listed fragments may be made to improve or modify the endothelial cell proliferation inhibiting activity or anti-angiogenic activity of the angiostatin fragments. Such modifications are not intended to exceed the scope and spirit of the claims.
- the cysteine residues can be mutated to serines.
- angiostatin fragments which do not significantly alter the fragments' endothelial cell proliferation inhibiting activity, and which are, therefore, not intended to exceed the scope of the claims.
- angiostatin fragment has at least 60%, more preferably at least 70%, and more preferably at least 80% of the endothelial cell proliferation inhibiting activity compared to that of the closest homologous angiostatin fragment disclosed herein.
- Kringle 1-5 angiostatin protein fragment can be produced according to the following method:
- step 5 Run the product of step 4 over a lysine-Sepharose column to purify Kl-5 from the rest.
- the kringle 1-5 angiostatin protein fragment can be used to inhibit endothelial cell proliferation and angiogenesis in vitro and in vivo.
- the kringle 1-5 angiostatin protein fragment can be used to inhibit angiogenesis in a cancerous tumor.
- ADDRESSEE Jones & Askew
- B STREET: 191 Peachtree Street, 37th Floor
- Gly Gin Gly Asp Ser Leu Asp Gly Tyr lie Ser Thr Gin Gly Ala Ser 20 25 30
- Tyr Glu Gly Lys lie Ser Lys Thr Met Ser Gly Leu Asp Cys Gin Ala 195 200 205 Trp Asp Ser Gin Ser Pro His Ala His Gly Tyr lie Pro Ala Lys Phe
- Trp Pro Trp Gin lie Ser Leu Arg Thr Arg Phe Thr Gly Gin His Phe 595 600 605
- Phe Glu Lys Asp Lys Tyr lie Leu Gin Gly Val Thr Ser Trp Gly Leu 770 775 780
- Glu Gly Lys lie Ser Lys Thr Met Ser Gly Leu Asp Cys Gin Ala Trp 100 105 110
- Asp Gly Lys lie Ser Lys Thr Met Ser Gly Leu Glu Cys Gin Ala Trp 100 105 110
- MOLECULE TYPE protein
- HYPOTHETICAL NO
- ORIGINAL SOURCE
- Asp Gly Lys lie Ser Lys Thr Met Ser Gly Leu Glu Cys Gin Ala Trp 100 105 110
- MOLECULE TYPE protein
- HYPOTHETICAL NO
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA002291892A CA2291892C (en) | 1997-05-30 | 1998-05-29 | Angiostatin fragments and method of use |
EP98925007A EP0996632A4 (en) | 1997-05-30 | 1998-05-29 | Angiostatin fragments and method of use |
JP50095299A JP2001506506A (en) | 1997-05-30 | 1998-05-29 | Angiostatin fragments and methods of use |
AU77049/98A AU744671B2 (en) | 1994-04-26 | 1998-05-29 | Angiostatin fragments and method of use |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/866,735 | 1997-05-30 | ||
US08/866,735 US5945403A (en) | 1997-05-30 | 1997-05-30 | Angiostatin fragments and method of use |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1998054217A1 true WO1998054217A1 (en) | 1998-12-03 |
Family
ID=25348292
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1998/010979 WO1998054217A1 (en) | 1994-04-26 | 1998-05-29 | Angiostatin fragments and method of use |
Country Status (6)
Country | Link |
---|---|
US (5) | US5945403A (en) |
EP (1) | EP0996632A4 (en) |
JP (1) | JP2001506506A (en) |
KR (1) | KR20010020458A (en) |
CA (1) | CA2291892C (en) |
WO (1) | WO1998054217A1 (en) |
Cited By (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999029878A2 (en) * | 1997-12-08 | 1999-06-17 | Beth Israel Deaconess Medical Center | Methods of producing anti-angiogenic proteins: endostatin, angiostatin or restin, using a pichia yeast expression system |
WO2000032631A2 (en) * | 1998-12-04 | 2000-06-08 | Entremed, Inc. | Proteins that bind angiogenesis-inhibiting proteins, compositions and methods of use thereof |
WO2000044908A2 (en) * | 1999-02-01 | 2000-08-03 | Beth Israel Deaconess Medical Center | Comp/tsp-1, comp/tsp-2 and other tsp chimeric proteins |
WO2000044391A2 (en) * | 1999-01-28 | 2000-08-03 | The Children's Medical Center Corporation | Plasminogen kringle 4 region fragments and methods of use |
WO2000047729A1 (en) * | 1999-02-10 | 2000-08-17 | Entremed, Inc. | Deglycosylated kringle 1-5 region fragments of plasminogen and methods of use |
WO2000061179A1 (en) * | 1999-04-14 | 2000-10-19 | Karolinska Innovations Ab | Kringle domains of plasminogen, capable of modulating angiogenesis in vivo |
KR20020008471A (en) * | 2000-07-20 | 2002-01-31 | 김승수 | Human prothrombin kringle with endothelial cell growth inhibitory activity, vector and host thereof |
WO2002017857A2 (en) * | 2000-09-01 | 2002-03-07 | Philadelphia, Health And Education Corporation | Methods and compositions for inhibiting angiogenesis |
WO2002020813A2 (en) * | 2000-09-05 | 2002-03-14 | Karolinska Innovations Ab | Recombinant endothelial cell growth inhibitors derived from a mammalian plasminogen |
US6358735B1 (en) | 1995-06-30 | 2002-03-19 | University Of Kansas Medical Center | Method for inhibiting angiogenesis and tumors with the isolated NC1 α3 chain monomer of type IV collagen |
US6361994B1 (en) | 1995-06-30 | 2002-03-26 | University Of Kansas Medical Center | Method for inhibiting angiogenesis and tumors with the isolated NC1 α1 chain monomer of type IV collagen |
US6576609B1 (en) | 1996-09-17 | 2003-06-10 | Northwestern University | Methods and compositions for generating angiostatin |
US6797488B1 (en) | 1997-12-08 | 2004-09-28 | Beth Israel Deaconess Medical Center | Methods of producing anti-angiogenic proteins |
WO2004108916A1 (en) * | 2003-06-06 | 2004-12-16 | Pfizer Products Inc. | Methods and compositions for diagnosing and treating disorders involving angiogenesis |
WO2005079835A1 (en) * | 2004-02-24 | 2005-09-01 | Ttc Co., Ltd. | Anticancer agent containing bl angiostatin |
US7144854B1 (en) | 1999-09-10 | 2006-12-05 | Conjuchem, Inc. | Long lasting anti-angiogenic peptides |
US7157556B1 (en) | 1999-02-10 | 2007-01-02 | The Children's Medical Center Corporation | Deglycosylated kringle 1-3 region fragments of plasminogen and methods of use |
US7160858B2 (en) | 2000-09-01 | 2007-01-09 | Philadelphia, Health And Education Corporation | Methods and compositions for inhibiting angiogenesis |
US7741286B2 (en) | 1999-05-17 | 2010-06-22 | Conjuchem Biotechnologies Inc. | Long lasting anti-angiogenic peptides |
Families Citing this family (43)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6949511B1 (en) * | 1994-04-26 | 2005-09-27 | The Children's Medical Center Corporation | Methods of inhibiting angiogenesis via increasing in vivo concentrations of kringle region fragments of plasminogen |
US5945403A (en) * | 1997-05-30 | 1999-08-31 | The Children's Medical Center Corporation | Angiostatin fragments and method of use |
US5837682A (en) * | 1996-03-08 | 1998-11-17 | The Children's Medical Center Corporation | Angiostatin fragments and method of use |
US20040265288A1 (en) * | 1995-03-08 | 2004-12-30 | Gilman Michael Z. | New applications of gene therapy technology |
US6528483B2 (en) | 1995-06-07 | 2003-03-04 | André Beaulieu | Method of producing concentrated non-buffered solutions of fibronectin |
US6346510B1 (en) | 1995-10-23 | 2002-02-12 | The Children's Medical Center Corporation | Therapeutic antiangiogenic endostatin compositions |
US6475784B1 (en) * | 1997-11-14 | 2002-11-05 | Valentis, Inc. | Inhibition of angiogenesis by delivery of nucleic acids encoding anti-angiogenic polypeptides |
US20040022788A1 (en) * | 1998-05-19 | 2004-02-05 | Moser Tammy L. | Compositions and methods for promoting or inhibiting angiogenesis |
WO1999059620A1 (en) * | 1998-05-19 | 1999-11-25 | Duke University | Angiostatin receptor |
IL140729A0 (en) | 1998-07-14 | 2002-02-10 | Bristol Myers Squibb Co | Lysine binding fragments of angiostatin, pharmaceutical compositions containing the same and processes for the preparation thereof |
US6465424B1 (en) * | 1999-02-17 | 2002-10-15 | Bristol-Myers Squibb Company | Anti-angiogenic agent and method for inhibiting angiogenesis |
AU774759B2 (en) | 1999-09-15 | 2004-07-08 | Mogam Biotechnology Research Institute | A novel angiogenesis inhibitor |
US6979563B1 (en) | 1999-11-17 | 2005-12-27 | Biomarin Enzymes, Inc. | Attenuation of tumor growth, metastasis and angiogenesis |
US7361643B2 (en) | 2000-02-09 | 2008-04-22 | University Of Puerto Rico | Methods for inhibiting angiogenesis |
WO2001097752A2 (en) * | 2000-06-20 | 2001-12-27 | The Trustees Of The University Of Pennsylvania | Compositions comprising urokinase for modulating muscle contractility and angiogenisis |
WO2002044328A2 (en) | 2000-11-28 | 2002-06-06 | Waisman David M | Anti-angiogenic polypeptides |
US7122181B2 (en) * | 2000-12-19 | 2006-10-17 | Research Development Foundation | Lentiviral vector-mediated gene transfer and uses thereof |
US20030228298A1 (en) * | 2001-09-04 | 2003-12-11 | Mark Nesbit | Abrogen polypeptides, nucleic acids encoding them and methods for using them to inhibit angiogenesis |
US7285277B2 (en) * | 2002-03-15 | 2007-10-23 | Mogam Biotechnology Research Institute | Anticancer agent |
EP1539799B1 (en) * | 2002-06-21 | 2013-12-11 | The University of Utah Research Foundation | Crosslinked compounds and methods of making and using thereof |
US20040052777A1 (en) * | 2002-09-04 | 2004-03-18 | Mark Nesbit | Kringle polypeptides and methods for using them to inhibit angiogenesis |
EP1581164A4 (en) * | 2002-12-13 | 2008-03-12 | Mediomics Llc | Compositions and methods for inhibiting tumor growth and metastasis |
CN101137388A (en) | 2003-05-15 | 2008-03-05 | 犹他卅大学研究基金会 | Anti-adhesion composites and methods os use thereof |
US20050250694A1 (en) * | 2003-10-10 | 2005-11-10 | Ma Jian-Xing | Compounds useful in inhibiting vascular leakage, inflammation and fibrosis and methods of making and using same |
US7981871B2 (en) * | 2003-12-04 | 2011-07-19 | University Of Utah Research Foundation | Modified macromolescules and associated methods of synthesis and use |
US20100330143A1 (en) | 2003-12-04 | 2010-12-30 | University Of Utah Research Foundation | Modified macromolecules and methods of making and using thereof |
US20050130897A1 (en) * | 2003-12-11 | 2005-06-16 | Ma Jian-Xing | Compounds useful in inhibiting vascular leakage, inflammation and fibrosis and methods of making and using same |
ITMI20041962A1 (en) * | 2004-10-15 | 2005-01-15 | Istituto Naz Per La Ricerca S | "PEPTIDE OF ANGIOSTATIN AND ITS THERAPEUTIC EMPLOYEES" |
KR20080016780A (en) * | 2004-11-24 | 2008-02-22 | 테라킨 리미티드 | An implant for intraocular drug delivery |
EP2012803A4 (en) * | 2006-04-20 | 2012-08-01 | Univ Utah Res Found | Polymeric compositions and methods of making and using thereof |
US8747870B2 (en) | 2006-04-20 | 2014-06-10 | University Of Utah Research Foundation | Polymeric compositions and methods of making and using thereof |
US20080032920A1 (en) * | 2006-07-11 | 2008-02-07 | Prestwich Glenn D | Macromolecules modified with electrophilic groups and methods of making and using thereof |
US20080287341A1 (en) * | 2007-05-18 | 2008-11-20 | Danyang Chen | Treatment of vascular abnormalities using nanoparticles |
EP2435562A1 (en) | 2009-05-26 | 2012-04-04 | Biolex Therapeutics, Inc. | Compositions and methods for production of aglycosylated plasminogen |
CA2819181C (en) * | 2010-11-29 | 2020-03-10 | Dako Denmark A/S | Methods and systems for analyzing images of specimens processed by a programmable quantitative assay |
WO2013010045A1 (en) | 2011-07-12 | 2013-01-17 | Biotime Inc. | Novel methods and formulations for orthopedic cell therapy |
EP3054881A4 (en) | 2013-10-15 | 2017-06-14 | Autonomix Medical, Inc. | Systems and methods for treating cancer and/or augmenting organ function |
US9616114B1 (en) | 2014-09-18 | 2017-04-11 | David Gordon Bermudes | Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity |
WO2016090143A1 (en) | 2014-12-04 | 2016-06-09 | Autonomix Medical, Inc. | Systems and methods for treating cancer and/or augmenting organ function |
KR101760274B1 (en) | 2015-10-02 | 2017-07-21 | 최동운 | Sensitive smart jewelry based on context awareness |
JOP20190100A1 (en) | 2016-11-19 | 2019-05-01 | Potenza Therapeutics Inc | Anti-gitr antigen-binding proteins and methods of use thereof |
US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5639725A (en) * | 1994-04-26 | 1997-06-17 | Children's Hospital Medical Center Corp. | Angiostatin protein |
Family Cites Families (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5836391A (en) * | 1981-08-07 | 1983-03-03 | Sankyo Co Ltd | New physiologically active substance angiostatin |
WO1990013640A1 (en) | 1989-05-01 | 1990-11-15 | The University Of Notre Dame Du Lac | Methods and materials for expression of human plasminogen in a eukaryotic cell system |
GB8927722D0 (en) | 1989-12-07 | 1990-02-07 | British Bio Technology | Proteins and nucleic acids |
US5192744A (en) * | 1990-01-12 | 1993-03-09 | Northwestern University | Method of inhibiting angiogenesis of tumors |
WO1993016716A1 (en) | 1992-02-24 | 1993-09-02 | Northwestern University | Method and composition for inhibiting angiogenesis |
WO1993016717A1 (en) * | 1992-02-25 | 1993-09-02 | The Rockefeller University | Method and agents for promoting wound healing |
ATE274577T1 (en) * | 1993-10-06 | 2004-09-15 | Univ Florida | STEM CELL PROLIFERATION FACTOR |
US5753230A (en) * | 1994-03-18 | 1998-05-19 | The Scripps Research Institute | Methods and compositions useful for inhibition of angiogenesis |
EP0758390B1 (en) | 1994-04-26 | 2007-02-28 | The Children's Medical Center Corporation | Angiostatin and method of use for inhibition of angiogenesis |
US5837682A (en) | 1996-03-08 | 1998-11-17 | The Children's Medical Center Corporation | Angiostatin fragments and method of use |
US5945403A (en) | 1997-05-30 | 1999-08-31 | The Children's Medical Center Corporation | Angiostatin fragments and method of use |
US5861372A (en) | 1996-02-22 | 1999-01-19 | The Children's Medical Center Corporation | Aggregate angiostatin and method of use |
ATE368051T1 (en) * | 1995-04-26 | 2007-08-15 | Childrens Medical Center | ANGIOSTATIN FRAGMENTS AND METHODS OF USE THEREOF |
US5854221A (en) | 1996-12-12 | 1998-12-29 | The Children's Medical Center Corporation | Endothelial cell proliferation inhibitor and method of use |
US5801146A (en) * | 1996-05-03 | 1998-09-01 | Abbott Laboratories | Compound and method for inhibiting angiogenesis |
HU224827B1 (en) | 1996-05-03 | 2006-02-28 | Abbott Lab | Novel antiangiogenic peptides polynucleotides encoding same and methods for inhibiting angiogenesis |
US5801012A (en) * | 1996-09-17 | 1998-09-01 | Northwestern University | Methods and compositions for generating angiostatin |
JP2002510209A (en) * | 1997-06-26 | 2002-04-02 | カロリンスカ イノベイションズ アクチボラゲット | Kringle domain 1-5 of plasminogen that can regulate angiogenesis in vivo |
-
1997
- 1997-05-30 US US08/866,735 patent/US5945403A/en not_active Expired - Fee Related
-
1998
- 1998-05-29 KR KR1019997011209A patent/KR20010020458A/en not_active Application Discontinuation
- 1998-05-29 EP EP98925007A patent/EP0996632A4/en not_active Withdrawn
- 1998-05-29 JP JP50095299A patent/JP2001506506A/en active Pending
- 1998-05-29 WO PCT/US1998/010979 patent/WO1998054217A1/en not_active Application Discontinuation
- 1998-05-29 CA CA002291892A patent/CA2291892C/en not_active Expired - Fee Related
-
2001
- 2001-01-16 US US09/761,120 patent/US20020037847A1/en not_active Abandoned
- 2001-02-16 US US09/788,142 patent/US20010029246A1/en not_active Abandoned
-
2003
- 2003-03-27 US US10/401,108 patent/US7365159B2/en not_active Expired - Lifetime
- 2003-03-28 US US10/402,364 patent/US20040002459A1/en not_active Abandoned
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5639725A (en) * | 1994-04-26 | 1997-06-17 | Children's Hospital Medical Center Corp. | Angiostatin protein |
US5733876A (en) * | 1994-04-26 | 1998-03-31 | The Children's Medical Center Corporation | Method of inhibiting angiogenesis |
Non-Patent Citations (1)
Title |
---|
See also references of EP0996632A4 * |
Cited By (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6358735B1 (en) | 1995-06-30 | 2002-03-19 | University Of Kansas Medical Center | Method for inhibiting angiogenesis and tumors with the isolated NC1 α3 chain monomer of type IV collagen |
US6440729B1 (en) | 1995-06-30 | 2002-08-27 | University Of Kansas Medical Center | Treating angiogenesis-mediated diseases with the α2 monomer of type IV collagen |
US6432706B1 (en) | 1995-06-30 | 2002-08-13 | University Of Kansas Medical Center | Method for inhibiting angiogenesis and tumors with the isolated NC1 α6 chain monomer of type IV collagen |
US6361994B1 (en) | 1995-06-30 | 2002-03-26 | University Of Kansas Medical Center | Method for inhibiting angiogenesis and tumors with the isolated NC1 α1 chain monomer of type IV collagen |
US6576609B1 (en) | 1996-09-17 | 2003-06-10 | Northwestern University | Methods and compositions for generating angiostatin |
US6797488B1 (en) | 1997-12-08 | 2004-09-28 | Beth Israel Deaconess Medical Center | Methods of producing anti-angiogenic proteins |
WO1999029878A2 (en) * | 1997-12-08 | 1999-06-17 | Beth Israel Deaconess Medical Center | Methods of producing anti-angiogenic proteins: endostatin, angiostatin or restin, using a pichia yeast expression system |
WO1999029878A3 (en) * | 1997-12-08 | 1999-09-16 | Beth Israel Hospital | Methods of producing anti-angiogenic proteins: endostatin, angiostatin or restin, using a pichia yeast expression system |
AU753889B2 (en) * | 1997-12-08 | 2002-10-31 | Beth Israel Deaconess Medical Center | Methods of producing anti-angiogenic proteins: endostatin, angiostatin or restin, using a pichia yeast expression system |
WO2000032631A2 (en) * | 1998-12-04 | 2000-06-08 | Entremed, Inc. | Proteins that bind angiogenesis-inhibiting proteins, compositions and methods of use thereof |
WO2000032631A3 (en) * | 1998-12-04 | 2001-06-14 | Entremed Inc | Proteins that bind angiogenesis-inhibiting proteins, compositions and methods of use thereof |
WO2000044391A2 (en) * | 1999-01-28 | 2000-08-03 | The Children's Medical Center Corporation | Plasminogen kringle 4 region fragments and methods of use |
WO2000044391A3 (en) * | 1999-01-28 | 2000-12-21 | Childrens Medical Center | Plasminogen kringle 4 region fragments and methods of use |
WO2000044908A3 (en) * | 1999-02-01 | 2001-02-15 | Beth Israel Hospital | Comp/tsp-1, comp/tsp-2 and other tsp chimeric proteins |
WO2000044908A2 (en) * | 1999-02-01 | 2000-08-03 | Beth Israel Deaconess Medical Center | Comp/tsp-1, comp/tsp-2 and other tsp chimeric proteins |
WO2000047729A1 (en) * | 1999-02-10 | 2000-08-17 | Entremed, Inc. | Deglycosylated kringle 1-5 region fragments of plasminogen and methods of use |
US7157556B1 (en) | 1999-02-10 | 2007-01-02 | The Children's Medical Center Corporation | Deglycosylated kringle 1-3 region fragments of plasminogen and methods of use |
WO2000061179A1 (en) * | 1999-04-14 | 2000-10-19 | Karolinska Innovations Ab | Kringle domains of plasminogen, capable of modulating angiogenesis in vivo |
US7741286B2 (en) | 1999-05-17 | 2010-06-22 | Conjuchem Biotechnologies Inc. | Long lasting anti-angiogenic peptides |
US7144854B1 (en) | 1999-09-10 | 2006-12-05 | Conjuchem, Inc. | Long lasting anti-angiogenic peptides |
KR20020008471A (en) * | 2000-07-20 | 2002-01-31 | 김승수 | Human prothrombin kringle with endothelial cell growth inhibitory activity, vector and host thereof |
WO2002017857A2 (en) * | 2000-09-01 | 2002-03-07 | Philadelphia, Health And Education Corporation | Methods and compositions for inhibiting angiogenesis |
US7625868B2 (en) | 2000-09-01 | 2009-12-01 | Philadelphia, Health And Education Corporation | Methods and compositions for inhibiting angiogenesis |
US7160858B2 (en) | 2000-09-01 | 2007-01-09 | Philadelphia, Health And Education Corporation | Methods and compositions for inhibiting angiogenesis |
WO2002017857A3 (en) * | 2000-09-01 | 2002-06-13 | Philadelphia Health & Educatio | Methods and compositions for inhibiting angiogenesis |
WO2002020813A2 (en) * | 2000-09-05 | 2002-03-14 | Karolinska Innovations Ab | Recombinant endothelial cell growth inhibitors derived from a mammalian plasminogen |
US7008921B2 (en) | 2000-09-05 | 2006-03-07 | Karolinska Innovations Ab | Materials and methods relating to endothelial cell growth inhibitors |
WO2002020813A3 (en) * | 2000-09-05 | 2003-01-16 | Karolinska Innovations Ab | Recombinant endothelial cell growth inhibitors derived from a mammalian plasminogen |
WO2004108916A1 (en) * | 2003-06-06 | 2004-12-16 | Pfizer Products Inc. | Methods and compositions for diagnosing and treating disorders involving angiogenesis |
WO2005079835A1 (en) * | 2004-02-24 | 2005-09-01 | Ttc Co., Ltd. | Anticancer agent containing bl angiostatin |
Also Published As
Publication number | Publication date |
---|---|
EP0996632A1 (en) | 2000-05-03 |
EP0996632A4 (en) | 2004-10-20 |
US20010029246A1 (en) | 2001-10-11 |
CA2291892A1 (en) | 1998-12-03 |
US20040023877A1 (en) | 2004-02-05 |
US7365159B2 (en) | 2008-04-29 |
US20040002459A1 (en) | 2004-01-01 |
CA2291892C (en) | 2006-03-28 |
US5945403A (en) | 1999-08-31 |
US20020037847A1 (en) | 2002-03-28 |
KR20010020458A (en) | 2001-03-15 |
JP2001506506A (en) | 2001-05-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US6521439B2 (en) | Nucleic acids encoding plasminogen fragments | |
US5945403A (en) | Angiostatin fragments and method of use | |
AU709633B2 (en) | Angiostatin fragments and aggregate angiostatin and methods of use | |
US5885795A (en) | Methods of expressing angiostatic protein | |
US5861372A (en) | Aggregate angiostatin and method of use | |
EP1783215B1 (en) | Angiostatin and method of use for inhibition of angiogenesis | |
EP0869970B1 (en) | Endothelial cell proliferation inhibitor and its use | |
US6949511B1 (en) | Methods of inhibiting angiogenesis via increasing in vivo concentrations of kringle region fragments of plasminogen | |
AU744671B2 (en) | Angiostatin fragments and method of use | |
EP1867721A1 (en) | Angiostatin fragments and aggregate angiostatin and methods of use | |
AU4440402A (en) | Angiostatin fragments and method of use | |
MXPA99011041A (en) | Angiostatin fragments and method of use |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH GM GW HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN ML MR NE SN TD TG |
|
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
ENP | Entry into the national phase |
Ref document number: 2291892 Country of ref document: CA Kind code of ref document: A Country of ref document: CA |
|
ENP | Entry into the national phase |
Ref document number: 1999 500952 Country of ref document: JP Kind code of ref document: A |
|
WWE | Wipo information: entry into national phase |
Ref document number: PA/a/1999/011041 Country of ref document: MX Ref document number: 1019997011209 Country of ref document: KR |
|
WWE | Wipo information: entry into national phase |
Ref document number: 1998925007 Country of ref document: EP Ref document number: 77049/98 Country of ref document: AU |
|
REG | Reference to national code |
Ref country code: DE Ref legal event code: 8642 |
|
WWP | Wipo information: published in national office |
Ref document number: 1998925007 Country of ref document: EP |
|
WWP | Wipo information: published in national office |
Ref document number: 1019997011209 Country of ref document: KR |
|
WWG | Wipo information: grant in national office |
Ref document number: 77049/98 Country of ref document: AU |
|
WWR | Wipo information: refused in national office |
Ref document number: 1019997011209 Country of ref document: KR |