WO1998052411B1 - Cryopreservation of embryogenic callus - Google Patents
Cryopreservation of embryogenic callusInfo
- Publication number
- WO1998052411B1 WO1998052411B1 PCT/US1998/009557 US9809557W WO9852411B1 WO 1998052411 B1 WO1998052411 B1 WO 1998052411B1 US 9809557 W US9809557 W US 9809557W WO 9852411 B1 WO9852411 B1 WO 9852411B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- plant
- caiius
- callus
- piant
- placing
- Prior art date
Links
- 206010020649 Hyperkeratosis Diseases 0.000 title claims abstract 12
- 238000005138 cryopreservation Methods 0.000 title 1
- 230000000408 embryogenic Effects 0.000 title 1
- 238000010257 thawing Methods 0.000 claims abstract 4
- 241000196324 Embryophyta Species 0.000 claims 16
- 240000008042 Zea mays Species 0.000 claims 4
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims 4
- 235000009973 maize Nutrition 0.000 claims 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims 4
- 239000012047 saturated solution Substances 0.000 claims 3
- FBPFZTCFMRRESA-KAZBKCHUSA-N D-Mannitol Natural products OC[C@@H](O)[C@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KAZBKCHUSA-N 0.000 claims 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N D-sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims 2
- 240000007842 Glycine max Species 0.000 claims 2
- 235000010469 Glycine max Nutrition 0.000 claims 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims 2
- 240000007594 Oryza sativa Species 0.000 claims 2
- 235000007164 Oryza sativa Nutrition 0.000 claims 2
- 240000006394 Sorghum bicolor Species 0.000 claims 2
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims 2
- CZMRCDWAGMRECN-GDQSFJPYSA-N Sucrose Natural products O([C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O1)[C@@]1(CO)[C@H](O)[C@@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-GDQSFJPYSA-N 0.000 claims 2
- 240000008529 Triticum aestivum Species 0.000 claims 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims 2
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Inorganic materials [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 claims 2
- 239000003365 glass fiber Substances 0.000 claims 2
- 239000007788 liquid Substances 0.000 claims 2
- 239000000594 mannitol Substances 0.000 claims 2
- 235000010355 mannitol Nutrition 0.000 claims 2
- 229910052757 nitrogen Inorganic materials 0.000 claims 2
- 235000009566 rice Nutrition 0.000 claims 2
- 239000005720 sucrose Substances 0.000 claims 2
- 235000021307 wheat Nutrition 0.000 claims 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N Ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N D-Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims 1
- 239000002202 Polyethylene glycol Substances 0.000 claims 1
- 239000000835 fiber Substances 0.000 claims 1
- 239000008103 glucose Substances 0.000 claims 1
- 238000007654 immersion Methods 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
- 229920001223 polyethylene glycol Polymers 0.000 claims 1
- 239000000600 sorbitol Substances 0.000 claims 1
- 210000001519 tissues Anatomy 0.000 claims 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N β-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims 1
- 230000001082 cryoprotectant Effects 0.000 abstract 1
- 239000002577 cryoprotective agent Substances 0.000 abstract 1
- 241000894007 species Species 0.000 abstract 1
Abstract
A method for cryopreserving plant callus without the use of cryoprotectants or programmable freezers. The callus is spread on a physical support, grown on a media supplemented with an osmoticum, desiccated under controlled conditions, placed directly into cold storage, and can be revived by thawing. Actively growing callus from a number of species can be cryopreserved using the instant invention.
Claims
[received by the International Bureau on 10 November 1998 (10.11.98); original claims 1-15 replaced by new claims 1-14 (2 pages)]
1.
A method of cryopreserving plant callus comprising the steps of spreading the plant callus evenly on a physical support; cuituring said plant cailus on a maintenance medium suppiemented with an osmoticum; desiccating said piant callus under controlled conditions; placing said desiccated piant callus in storage.
2.
The method of claim 1 wherein the stored plant callus is revived by thawing.
3. The method of claim 1 wherein the physical support is selected from the group consisting of a glass fiber disc, a paper disc, a fine wire mesh, and miracloth.
4.
The method of claim 3 wherein the physical support is a glass fiber disc.
5. The method of claim 1 wherein the maintenance medium is supplemented with proiine.
6.
The method of claim 5 wherein the osmoticum is selected from the group consisting of mannitol, sucrose, glucose, sorbitol , and polyethylene glycol.
7.
The method of claim 1 wherein the controlled conditions for desiccation are selected from the group consisting of placing the piant callus in a chamber in the presence of a saturated solution of Ca(NO3)24H2O, placing the plant caiius in a chamber in the presence of a saturated solution of (NH4)2S04, and placing the plant caiius in a mechanical device which aiters ambient humidity.
8.
The method of claim 1 wherein the storage comprises submersion of the plant tissue in liquid nitrogen.
9. The method of claim 2 wherein the reviving procedure comprises thawing at room temperature.
10.
A method of cryopreserving plant caiius comprising the steps of:
A. spreading the plant caiius evenly on a giass fiber disc; B. cuituring the piant caiius on a maintenance medium suppiemented with mannitol or sucrose, and proiine;
C. desiccating the plant caiius by exposure to a saturated solution of Ca(NO3)24H2O or (NH4)2SO4;
D. placing the desiccated plant caiius of C in cold storage by immersion in liquid nitrogen; E. reviving the stored piant callus by thawing the plant caiius at room temperature.
11. The method of ciaim 1 wherein the plant caiius is selected from the group consisting of maize, sorghum, wheat, soybean, and rice.
12.
The method of ciaim 11 wherein the plant caiius is Type II maize caiius.
13. The method of ciaim 9 wherein the plant caiius is selected from the group consisting of maize, sorghum, wheat, soybean, and rice.
14.
The method of claim 13 wherein the plant callus is Type ii maize caiius.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP98921106A EP0982985A2 (en) | 1997-05-20 | 1998-05-11 | Cryopreservation of embryogenic callus |
| AU73783/98A AU7378398A (en) | 1997-05-20 | 1998-05-11 | Cryopreservation of embryogenic callus |
| BR9808805-0A BR9808805A (en) | 1997-05-20 | 1998-05-11 | Method of cryopreserving plant callus |
| CA002289947A CA2289947A1 (en) | 1997-05-20 | 1998-05-11 | Cryopreservation of embryogenic callus |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/859,313 | 1997-05-20 | ||
| US08/859,313 US6143563A (en) | 1997-05-20 | 1997-05-20 | Cryopreservation of embryogenic callus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1998052411A1 WO1998052411A1 (en) | 1998-11-26 |
| WO1998052411B1 true WO1998052411B1 (en) | 1999-01-14 |
Family
ID=25330585
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1998/009557 WO1998052411A1 (en) | 1997-05-20 | 1998-05-11 | Cryopreservation of embryogenic callus |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US6143563A (en) |
| EP (1) | EP0982985A2 (en) |
| AU (1) | AU7378398A (en) |
| BR (1) | BR9808805A (en) |
| CA (1) | CA2289947A1 (en) |
| WO (1) | WO1998052411A1 (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6548263B1 (en) * | 1997-05-29 | 2003-04-15 | Cellomics, Inc. | Miniaturized cell array methods and apparatus for cell-based screening |
| EP1119246B1 (en) * | 1998-10-07 | 2002-07-31 | Societe Des Produits Nestle S.A. | Novel process for the cryo-preservation of plants |
| KR100845877B1 (en) | 2007-04-05 | 2008-07-14 | 인하대학교 산학협력단 | The method of cryopreservation for transgenic rice suspension cell |
| CN102763591B (en) * | 2012-07-05 | 2013-11-13 | 中国农业科学院作物科学研究所 | Atrac tylodes macrocephala koidz conservation method and re-cultivating method |
| CN103202225B (en) * | 2013-03-11 | 2014-07-30 | 上海市农业生物基因中心 | Ultralow-temperature storing, thawing and recultivating method for micro-stem tips of plants |
| CN111657272B (en) * | 2020-06-23 | 2022-05-24 | 上海市农业生物基因中心 | Ultralow-temperature preservation method for rice callus |
| CN113854283A (en) * | 2021-09-06 | 2021-12-31 | 中国林业科学研究院亚热带林业研究所 | Ultralow-temperature cryopreservation method for olive embryogenic callus |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4615141A (en) * | 1984-08-14 | 1986-10-07 | Purdue Research Foundation | Process for encapsulating asexual plant embryos |
| KR900007085B1 (en) * | 1985-10-22 | 1990-09-28 | 플랜트 제네틱스, 인코포레이티드 | Methods for enhanced somatic enbryogenesis |
| US4777762A (en) * | 1986-01-07 | 1988-10-18 | Plant Genetics, Inc. | Desiccated analogs of botanic seed |
| GB8716039D0 (en) * | 1987-07-08 | 1987-08-12 | Cell Systems Ltd | Biological chilling protection |
| EP0332581B1 (en) * | 1988-03-08 | 1996-12-11 | Ciba-Geigy Ag | Regeneration of fertile graminaceous plants of the subfamily pooideae from protoplasts |
| US5183757A (en) * | 1989-08-01 | 1993-02-02 | British Columbia Research Corporation | Process for the production, desiccation and germination of conifer somatic embryos |
| US7705215B1 (en) * | 1990-04-17 | 2010-04-27 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| DE4013099A1 (en) * | 1990-04-25 | 1991-10-31 | Hoechst Ag | Transforming immature somatic plant, esp. maize, embryos - by treating, in dry state, with nucleic acid soln., esp. for introducing resistance to phosphinothricin |
| AU679435B2 (en) * | 1991-12-19 | 1997-07-03 | University Of Saskatchewan | Maturation, desiccation and encapsulation of gymnosperm somatic embryos |
| JPH07507443A (en) * | 1992-01-21 | 1995-08-24 | コーベ ラボラトリーズ インコーポレイテッド | Methods of freezing cells and cell-like material |
| CA2180980A1 (en) * | 1994-01-13 | 1995-07-20 | Franciscus Abraham Antonius Tetteroo | Method for the production of desiccation tolerant plant embryoids and methods for germination of desiccation tolerant embryoids |
-
1997
- 1997-05-20 US US08/859,313 patent/US6143563A/en not_active Expired - Fee Related
-
1998
- 1998-05-11 AU AU73783/98A patent/AU7378398A/en not_active Abandoned
- 1998-05-11 EP EP98921106A patent/EP0982985A2/en not_active Ceased
- 1998-05-11 BR BR9808805-0A patent/BR9808805A/en not_active Application Discontinuation
- 1998-05-11 WO PCT/US1998/009557 patent/WO1998052411A1/en not_active Application Discontinuation
- 1998-05-11 CA CA002289947A patent/CA2289947A1/en not_active Abandoned
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Halmagyi et al. | Cryopreservation of Chrysanthemum morifolium (Dendranthema grandiflora Ramat.) using different approaches | |
| Lopushinsky et al. | Effect of soil temperature on root and shoot growth and on budburst timing in conifer seedling transplants | |
| Uragami et al. | Cryopreservation of dried axillary buds from plantlets of Asparagus officinalis L. grown in vitro | |
| Sakai | Plant cryopreservation | |
| US6180405B1 (en) | Starvation and storage of mature somatic embryos | |
| Gupta et al. | Cryopreservation of shoot tips of blackberry and raspberry by encapsulation-dehydration and vitrification | |
| Langens-Gerrits et al. | Hot-water treatment before tissue culture reduces initial contamination in Lilium and Acer | |
| Coste et al. | In vitro propagation and cryopreservation of Romanian endemic and rare Hypericum species | |
| Walters et al. | Cryopreservation of recalcitrant (ie desiccation-sensitive) seeds | |
| Goveia et al. | Developmental status is a critical factor in the selection of excised recalcitrant axes as explants for cryopreservation: a study on Trichilia dregeana Sond. | |
| WO1998052411B1 (en) | Cryopreservation of embryogenic callus | |
| Suzuki et al. | Cryopreservation of encapsulated gentian axillary buds following 2 step-preculture with sucrose and desiccation | |
| Watts | Leaf extension in Zea mays: I. leaf extension and water potential in relation to root-zone and air temperatures | |
| Arizaga et al. | Cryopreservation of in vitro shoot tips of ulluco (Ullucus tuberosus Cal.) using D cryo-plate method | |
| Bajaj | Casava plants from meristem cultures freeze-preserved for three years | |
| Engelmann | Present development and use of in vitro culture techniques for the conservation of plant genetic resources | |
| Engelmann | In vitro conservation of horticultural genetic resources review of the state of the art | |
| US6143563A (en) | Cryopreservation of embryogenic callus | |
| Ekinci et al. | Medium-and long-term conservation of ornamental plants using synthetic seed technology | |
| CN112997972A (en) | Ornitus avidus egg preservation method suitable for large-scale breeding | |
| Halmagvi et al. | Cryopreservation of Rosa shoot tips: importance of preculture conditions | |
| Benson | Cryopreservation of shoot-tips and meristems | |
| MyCock et al. | Cryopreservation of somatic embryoids of Phoenix dactylifera | |
| Normah et al. | Cryopreservation of seeds and embryonic axes of several Citrus species | |
| Ishikawa | Recent progress in cryopreservation of plant genetic resources |