WO1998049251A1 - Multiple rare-earth metal complexes - Google Patents
Multiple rare-earth metal complexes Download PDFInfo
- Publication number
- WO1998049251A1 WO1998049251A1 PCT/EP1998/002360 EP9802360W WO9849251A1 WO 1998049251 A1 WO1998049251 A1 WO 1998049251A1 EP 9802360 W EP9802360 W EP 9802360W WO 9849251 A1 WO9849251 A1 WO 9849251A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- ion
- rare
- earth metal
- complex
- arene
- Prior art date
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- 229910052761 rare earth metal Inorganic materials 0.000 title claims abstract description 40
- 150000002910 rare earth metals Chemical class 0.000 title description 4
- 230000000536 complexating effect Effects 0.000 claims abstract description 8
- 230000003287 optical effect Effects 0.000 claims abstract description 7
- 238000009007 Diagnostic Kit Methods 0.000 claims abstract description 3
- 229910052693 Europium Inorganic materials 0.000 claims description 3
- 229910052779 Neodymium Inorganic materials 0.000 claims description 3
- OGPBJKLSAFTDLK-UHFFFAOYSA-N europium atom Chemical compound [Eu] OGPBJKLSAFTDLK-UHFFFAOYSA-N 0.000 claims description 3
- QEFYFXOXNSNQGX-UHFFFAOYSA-N neodymium atom Chemical compound [Nd] QEFYFXOXNSNQGX-UHFFFAOYSA-N 0.000 claims description 3
- 229910052691 Erbium Inorganic materials 0.000 claims description 2
- 229910052689 Holmium Inorganic materials 0.000 claims description 2
- 229910052771 Terbium Inorganic materials 0.000 claims description 2
- 229910052775 Thulium Inorganic materials 0.000 claims description 2
- 229910052769 Ytterbium Inorganic materials 0.000 claims description 2
- UYAHIZSMUZPPFV-UHFFFAOYSA-N erbium Chemical compound [Er] UYAHIZSMUZPPFV-UHFFFAOYSA-N 0.000 claims description 2
- KJZYNXUDTRRSPN-UHFFFAOYSA-N holmium atom Chemical compound [Ho] KJZYNXUDTRRSPN-UHFFFAOYSA-N 0.000 claims description 2
- NAWDYIZEMPQZHO-UHFFFAOYSA-N ytterbium Chemical compound [Yb] NAWDYIZEMPQZHO-UHFFFAOYSA-N 0.000 claims description 2
- 229910052727 yttrium Inorganic materials 0.000 claims description 2
- GZCRRIHWUXGPOV-UHFFFAOYSA-N terbium atom Chemical compound [Tb] GZCRRIHWUXGPOV-UHFFFAOYSA-N 0.000 claims 1
- VWQVUPCCIRVNHF-UHFFFAOYSA-N yttrium atom Chemical compound [Y] VWQVUPCCIRVNHF-UHFFFAOYSA-N 0.000 claims 1
- -1 rare-earth metal ion Chemical class 0.000 abstract description 41
- JHFPQYFEJICGKC-UHFFFAOYSA-N erbium(3+) Chemical compound [Er+3] JHFPQYFEJICGKC-UHFFFAOYSA-N 0.000 abstract 1
- LNBHUCHAFZUEGJ-UHFFFAOYSA-N europium(3+) Chemical compound [Eu+3] LNBHUCHAFZUEGJ-UHFFFAOYSA-N 0.000 abstract 1
- HKCRVXUAKWXBLE-UHFFFAOYSA-N terbium(3+) Chemical compound [Tb+3] HKCRVXUAKWXBLE-UHFFFAOYSA-N 0.000 abstract 1
- AWSFICBXMUKWSK-UHFFFAOYSA-N ytterbium(3+) Chemical compound [Yb+3] AWSFICBXMUKWSK-UHFFFAOYSA-N 0.000 abstract 1
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 93
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 66
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 45
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 40
- 125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 description 40
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 33
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 33
- GQPLZGRPYWLBPW-UHFFFAOYSA-N calix[4]arene Chemical compound C1C(C=2)=CC=CC=2CC(C=2)=CC=CC=2CC(C=2)=CC=CC=2CC2=CC=CC1=C2 GQPLZGRPYWLBPW-UHFFFAOYSA-N 0.000 description 33
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 32
- 241001120493 Arene Species 0.000 description 22
- 239000007983 Tris buffer Substances 0.000 description 22
- 150000002500 ions Chemical class 0.000 description 19
- 125000003917 carbamoyl group Chemical group [H]N([H])C(*)=O 0.000 description 18
- 239000000203 mixture Substances 0.000 description 18
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 18
- 239000002904 solvent Substances 0.000 description 17
- 238000006243 chemical reaction Methods 0.000 description 16
- 150000001875 compounds Chemical class 0.000 description 16
- 229910000027 potassium carbonate Inorganic materials 0.000 description 16
- 239000000243 solution Substances 0.000 description 16
- 230000005284 excitation Effects 0.000 description 15
- 238000000034 method Methods 0.000 description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 15
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 12
- 230000015572 biosynthetic process Effects 0.000 description 12
- 238000001953 recrystallisation Methods 0.000 description 12
- 125000006850 spacer group Chemical group 0.000 description 12
- 229910021644 lanthanide ion Inorganic materials 0.000 description 11
- 238000003786 synthesis reaction Methods 0.000 description 11
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 10
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 10
- UHOVQNZJYSORNB-UHFFFAOYSA-N monobenzene Natural products C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 10
- 150000002148 esters Chemical group 0.000 description 9
- 125000003754 ethoxycarbonyl group Chemical group C(=O)(OCC)* 0.000 description 9
- GAGGCOKRLXYWIV-UHFFFAOYSA-N europium(III) nitrate Inorganic materials [Eu+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O GAGGCOKRLXYWIV-UHFFFAOYSA-N 0.000 description 9
- 239000007787 solid Substances 0.000 description 9
- VGCXGMAHQTYDJK-UHFFFAOYSA-N Chloroacetyl chloride Chemical compound ClCC(Cl)=O VGCXGMAHQTYDJK-UHFFFAOYSA-N 0.000 description 8
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 8
- 238000004020 luminiscence type Methods 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 230000005281 excited state Effects 0.000 description 7
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 6
- 238000010521 absorption reaction Methods 0.000 description 6
- 230000003197 catalytic effect Effects 0.000 description 6
- 238000010668 complexation reaction Methods 0.000 description 6
- 238000005859 coupling reaction Methods 0.000 description 6
- 238000001704 evaporation Methods 0.000 description 6
- 230000008020 evaporation Effects 0.000 description 6
- CTSLXHKWHWQRSH-UHFFFAOYSA-N oxalyl chloride Chemical compound ClC(=O)C(Cl)=O CTSLXHKWHWQRSH-UHFFFAOYSA-N 0.000 description 6
- 239000000843 powder Substances 0.000 description 6
- 238000010992 reflux Methods 0.000 description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 5
- 238000005804 alkylation reaction Methods 0.000 description 5
- 230000008878 coupling Effects 0.000 description 5
- 238000010168 coupling process Methods 0.000 description 5
- 150000004985 diamines Chemical class 0.000 description 5
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- XFNJVJPLKCPIBV-UHFFFAOYSA-N trimethylenediamine Chemical compound NCCCN XFNJVJPLKCPIBV-UHFFFAOYSA-N 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 230000029936 alkylation Effects 0.000 description 4
- 239000012043 crude product Substances 0.000 description 4
- 239000013078 crystal Substances 0.000 description 4
- 239000003446 ligand Substances 0.000 description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 4
- 235000019341 magnesium sulphate Nutrition 0.000 description 4
- VLKZOEOYAKHREP-UHFFFAOYSA-N methyl pentane Natural products CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 4
- 239000002244 precipitate Substances 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- KIDHWZJUCRJVML-UHFFFAOYSA-N putrescine Chemical compound NCCCCN KIDHWZJUCRJVML-UHFFFAOYSA-N 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- CBCKQZAAMUWICA-UHFFFAOYSA-N 1,4-phenylenediamine Chemical compound NC1=CC=C(N)C=C1 CBCKQZAAMUWICA-UHFFFAOYSA-N 0.000 description 3
- DNMKJPPCIKKTNT-UHFFFAOYSA-N 2-chloro-n-[4-[(2-chloroacetyl)amino]butyl]acetamide Chemical compound ClCC(=O)NCCCCNC(=O)CCl DNMKJPPCIKKTNT-UHFFFAOYSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-MZCSYVLQSA-N Deuterated methanol Chemical compound [2H]OC([2H])([2H])[2H] OKKJLVBELUTLKV-MZCSYVLQSA-N 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 3
- 235000019270 ammonium chloride Nutrition 0.000 description 3
- 125000003118 aryl group Chemical group 0.000 description 3
- 238000004440 column chromatography Methods 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 230000001404 mediated effect Effects 0.000 description 3
- 239000012044 organic layer Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 229920006395 saturated elastomer Polymers 0.000 description 3
- 238000001228 spectrum Methods 0.000 description 3
- 239000011550 stock solution Substances 0.000 description 3
- DHQOZFQGPYEETI-UHFFFAOYSA-N tert-butyl n-[6-[(2-chloroacetyl)amino]hexyl]carbamate Chemical compound CC(C)(C)OC(=O)NCCCCCCNC(=O)CCl DHQOZFQGPYEETI-UHFFFAOYSA-N 0.000 description 3
- 238000010626 work up procedure Methods 0.000 description 3
- WZCQRUWWHSTZEM-UHFFFAOYSA-N 1,3-phenylenediamine Chemical compound NC1=CC=CC(N)=C1 WZCQRUWWHSTZEM-UHFFFAOYSA-N 0.000 description 2
- RKJYUCXSQOPWBA-UHFFFAOYSA-N 2-chloro-n-[4-[(2-chloroacetyl)amino]phenyl]acetamide Chemical compound ClCC(=O)NC1=CC=C(NC(=O)CCl)C=C1 RKJYUCXSQOPWBA-UHFFFAOYSA-N 0.000 description 2
- DLFVBJFMPXGRIB-UHFFFAOYSA-N Acetamide Chemical compound CC(N)=O DLFVBJFMPXGRIB-UHFFFAOYSA-N 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N Formic acid Chemical compound OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 229910017504 Nd(NO3)3 Inorganic materials 0.000 description 2
- NFHFRUOZVGFOOS-UHFFFAOYSA-N Pd(PPh3)4 Substances [Pd].C1=CC=CC=C1P(C=1C=CC=CC=1)C1=CC=CC=C1.C1=CC=CC=C1P(C=1C=CC=CC=1)C1=CC=CC=C1.C1=CC=CC=C1P(C=1C=CC=CC=1)C1=CC=CC=C1.C1=CC=CC=C1P(C=1C=CC=CC=1)C1=CC=CC=C1 NFHFRUOZVGFOOS-UHFFFAOYSA-N 0.000 description 2
- 239000005700 Putrescine Substances 0.000 description 2
- 229920005654 Sephadex Polymers 0.000 description 2
- 239000012507 Sephadex™ Substances 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- VTJUKNSKBAOEHE-UHFFFAOYSA-N calixarene Chemical class COC(=O)COC1=C(CC=2C(=C(CC=3C(=C(C4)C=C(C=3)C(C)(C)C)OCC(=O)OC)C=C(C=2)C(C)(C)C)OCC(=O)OC)C=C(C(C)(C)C)C=C1CC1=C(OCC(=O)OC)C4=CC(C(C)(C)C)=C1 VTJUKNSKBAOEHE-UHFFFAOYSA-N 0.000 description 2
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 2
- 238000010511 deprotection reaction Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000006260 foam Substances 0.000 description 2
- 235000019253 formic acid Nutrition 0.000 description 2
- NAQMVNRVTILPCV-UHFFFAOYSA-N hexane-1,6-diamine Chemical compound NCCCCCCN NAQMVNRVTILPCV-UHFFFAOYSA-N 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 150000003141 primary amines Chemical class 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- HXLSUUJYZLNLCS-UHFFFAOYSA-N tert-butyl n-[3-[(2-chloroacetyl)amino]propyl]carbamate Chemical compound CC(C)(C)OC(=O)NCCCNC(=O)CCl HXLSUUJYZLNLCS-UHFFFAOYSA-N 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 238000001665 trituration Methods 0.000 description 2
- 238000005160 1H NMR spectroscopy Methods 0.000 description 1
- MEKOFIRRDATTAG-UHFFFAOYSA-N 2,2,5,8-tetramethyl-3,4-dihydrochromen-6-ol Chemical compound C1CC(C)(C)OC2=C1C(C)=C(O)C=C2C MEKOFIRRDATTAG-UHFFFAOYSA-N 0.000 description 1
- 229920000858 Cyclodextrin Polymers 0.000 description 1
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 150000007933 aliphatic carboxylic acids Chemical class 0.000 description 1
- BHELZAPQIKSEDF-UHFFFAOYSA-N allyl bromide Chemical compound BrCC=C BHELZAPQIKSEDF-UHFFFAOYSA-N 0.000 description 1
- 125000003368 amide group Chemical group 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 239000012300 argon atmosphere Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000001273 butane Substances 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 150000001805 chlorine compounds Chemical class 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 229940097362 cyclodextrins Drugs 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000005595 deprotonation Effects 0.000 description 1
- 238000010537 deprotonation reaction Methods 0.000 description 1
- ZMXDDKWLCZADIW-UHFFFAOYSA-N dimethylformamide Substances CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 1
- 239000002019 doping agent Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000000921 elemental analysis Methods 0.000 description 1
- 238000003818 flash chromatography Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 238000007306 functionalization reaction Methods 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- OFBQJSOFQDEBGM-UHFFFAOYSA-N n-pentane Natural products CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 150000004032 porphyrins Chemical class 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000000425 proton nuclear magnetic resonance spectrum Methods 0.000 description 1
- GJAWHXHKYYXBSV-UHFFFAOYSA-N quinolinic acid Chemical class OC(=O)C1=CC=CN=C1C(O)=O GJAWHXHKYYXBSV-UHFFFAOYSA-N 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000001235 sensitizing effect Effects 0.000 description 1
- DKWSBNMUWZBREO-UHFFFAOYSA-N terbium Chemical compound [Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb][Tb] DKWSBNMUWZBREO-UHFFFAOYSA-N 0.000 description 1
- 150000001911 terphenyls Chemical class 0.000 description 1
- 238000004876 x-ray fluorescence Methods 0.000 description 1
- PRZWBGYJMNFKBT-UHFFFAOYSA-N yttrium Chemical compound [Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y][Y] PRZWBGYJMNFKBT-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F5/00—Compounds containing elements of Groups 3 or 13 of the Periodic Table
- C07F5/003—Compounds containing elements of Groups 3 or 13 of the Periodic Table without C-Metal linkages
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C235/00—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms
- C07C235/02—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to acyclic carbon atoms and singly-bound oxygen atoms bound to the same carbon skeleton
- C07C235/32—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to acyclic carbon atoms and singly-bound oxygen atoms bound to the same carbon skeleton the carbon skeleton containing six-membered aromatic rings
- C07C235/34—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to acyclic carbon atoms and singly-bound oxygen atoms bound to the same carbon skeleton the carbon skeleton containing six-membered aromatic rings having the nitrogen atoms of the carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C235/00—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms
- C07C235/02—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to acyclic carbon atoms and singly-bound oxygen atoms bound to the same carbon skeleton
- C07C235/32—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to acyclic carbon atoms and singly-bound oxygen atoms bound to the same carbon skeleton the carbon skeleton containing six-membered aromatic rings
- C07C235/38—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to acyclic carbon atoms and singly-bound oxygen atoms bound to the same carbon skeleton the carbon skeleton containing six-membered aromatic rings having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a six-membered aromatic ring
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/06—Luminescent, e.g. electroluminescent, chemiluminescent materials containing organic luminescent materials
Definitions
- the invention pertains to rare-earth metal ion complexes and optical devices and diagnostic kits comprising the same.
- Rare-earth ions are characterized by their long-lived excited state, which makes them eminently suited for optical amplification, wavelength conversion (“upconversion”) and sensitive diagnostics.
- the idea pertains to the use of multiple complexing units, such as calixarenes, hemispherands, polyaminocarboxyiic acids or ⁇ -diketonates, in combination with rare-earth ions of one type or of various types, with or without an attached (organic) sensitizer.
- the combination of rare-earth ions is of interest to enhance the excitation and/or emission possibilities of such ions (e.g., by broadening the range of absorption wavelengths) and to facilitate the use of multiple photons for excitation of these ions, with the additional possibility of wavelength conversion.
- the use of a sensitizer forms an additional advantage. It can be used to invoke much more efficient population of the excited state:
- the sensitizer has an absorption cross section which is typically 3-4 orders of magnitude higher than that of the rare-earth ion when, for instance, organic ⁇ - ⁇ transitions are considered.
- the lifetime of the excited state of the organic sensitizer generally is much shorter than the lifetime of the luminescent state of the rare-earth ion, multiple ions can be brought to the excited state by the same sensitizer. Therefore it is anticipated that a macromolecular unit consisting of several, preferably 2 or 3 complexing units, optionally, with attached rare-earth ions connected to at least one sensitizer, will serve as a very effective luminescent structure.
- Both the same rare-earth ions and a combination of different rare-earth ions can be applied. Unless use is made of a particular property to influence the complexation behavior of the ligands, a statistical mixture of various combinations of rare-earth ions will be obtained when more than one rare-earth ion is used.
- Preferred rare-earth metal ion complexes are complexes wherein the rare- earth metal ion is selected from Yb 3+ (ytterbium(lll) ion), Y 3+ (yttrium(lll) ion), Er 3+ (erbium(lll) ion), Tb 3+ (terbium(lll) ion), Tm 3+ (thulium(lll) ion), Ho 3+ (holmium(lll) ion), Eu 3+ (europium(lll) ion), and Nd 3+ (neodymium(lll) ion).
- the macroscopic complex can be synthesized at a specific pH to tune the complexation properties of the ligands: for instance, the pK values of aromatic carboxylic acids, aliphatic carboxylic acids, and pyridine dicarboxylic acids will be different. Further, to activate ⁇ -diketonate an alkaline treatment is required for deprotonation. When a specific pretreatment is applied to activate a selected part of the macroscopic structure, there may be rare-earth ion exchange.
- the absorption coefficients of lanthanide ions are usually low due to their weak absorption bands as compared to, e.g., organic molecules.
- Yb 3+ which is often used in lasers and optical amplifiers as a co-dopant, has a somewhat higher absorption coefficient than other rare-earth ions around 980 nm, where relatively inexpensive powerful lasers are available. Therefore, it can be used for excitation and subsequent transfer of its energy to another lanthanide ion which emits light in a wavelength region that is interesting for application in optical telecommunications, e.g., Er 3+ , which emits around 1530 nm.
- the application of this technique in current telecommunications networks renders energy transfer studies from one lanthanide ion to another important.
- the energy transfer studies can be performed by detection of the luminescence of the acceptor lanthanide ion, or by a decrease of the lifetime of the luminescent excited state of the donor ion. The latter method is most frequently used because it is easily accessible.
- Frequency upconversion can also be realized by excited state absorption processes, due to the relatively long- living excited state of rare-earth ions.
- inorganic crystals containing Y 3+ and Yb 3+ in combination with Er 3+ , Ho 3+ , or Tm 3+ have been applied. Excitation is generally done via Yb 3+ in the 950-1000 nm range; particularly the wavelength region around 980 nm, where relatively inexpensive powerful laser sources are available, is suitable for excitation.
- Emission wavelengths depend on the composition and structure of the inorganic crystals. Emission bands in the blue (around 400 nm), green (450-500 nm), orange (530-560 nm), and red (630-690 nm) parts of the spectrum have been reported.
- wavelength conversion materials are expected in two areas: (1) for optical telecommunications, when one desires to convert near-IR light (as used for high transmission long distance links) to visible light (which is transmitted most efficiently by low cost, polymer based short distance networks, e.g., in the home), and (2) for sensitive diagnostics where excitation can be done in the near-IR where no interference occurs with constituents of bodily fluids or specimens and emission is effected in the visible part of the spectrum, where the most sensitive detectors presently available have their optimum sensitivity.
- Suitable complexing moieties are macrocyclic structures, in particular calix[4]arenes.
- Calix[4]arenes are well-known building blocks in supramolecular chemistry because of their relatively easy functionalization. They consist of four phenolic rings that are linked via methylene bridges and can adopt different conformations. The cone conformation is the most stable one. The phenolic groups are all at the same face of the molecule (lower rim) and form a cyclic array of intramolecular hydrogen bonds in this conformation. As a consequence of the vase-like structure of the cone conformation and the presence of four aromatic units, this building block is called the calix[4]arene ("calix” or "chalise” is Greek for vase).
- the number of linked aromatic units can vary from 4 to 8, and is indicated by the number between brackets.
- Calix[4]arenes have been combined with several other building blocks, like resorcinarenes, cyclodextrins, terphenyls, and porphyrins, with the intention to build larger structures. This objective can also be achieved by the covalent coupling of two or more calix[4]arenes, either by lower-lower, lower-upper, or upper-upper rim linkages.
- biscalix[4]arenes that contain two molecular cages suitable for lanthanide ion complexation can be used.
- the hexaester biscalix[4]arene for instance, can be synthesized according to McKervey et al., Anqew. Chem. Int. Ed. Engl., 1990, (29), 280 and contains a sufficient number of donor atoms.
- biscalix[4]arenes containing amide groups instead of ester functions were synthesized. The synthesis routes leading to hexaesters and hexaamides both start from p-ter--butylcalix[4]arene and consist of four reaction steps.
- the combination of differently functionalized calix[4]arenes leads to non-symmetric biscalix[4]arenes that may be capable of selective complexation of different lanthanide ions.
- the synthesis route leading to biscalix[4]arene hexaamides starts with the monoprotection of p-ferf-butylcalix[4]arene (4) with 3-bromo-1-propene in N.N'-dimethylformamide using CsF as a base.
- the monoprotection was performed via a slightly modified literature procedure (L.C. Groenen, Conformational Properties of Calixarenes, PhD Thesis, University of Twente 1992).
- Calix[4]arene (8) was trialkylated with 2-chloro-N,N'-diethylacetamide in acetonitrile in the presence of a catalytic amount of potassium iodide and potassium carbonate as a base.
- the product was purified by recrystallization from acetonitrile and obtained in
- spacer units (11b), (11e), and (11f) were prepared by reaction of the corresponding diamine with chloroacetyl chloride in a mixture of ethyl acetate and water, using potassium carbonate as a base. These spacers were obtained in 70-79% yield, and characterized by 1 H NMR spectroscopy. Finally, the hexaamides (12b,e,f) were prepared via alkylation of (10) with 0.5 mole equivalents of spacer unit 11), a catalytic amount of potassium iodide, and potassium carbonate as a base.
- the reaction was carried out using chloroacetyl chloride (4.28 ml, 55 mmoles), 1 ,6-diaminohexane (2.00 g, 17.2 mmole), and potassium carbonate (10.5 g, 76 mmole) in ethyl acetate (60 ml) and water (60 ml), leading to (11f) in 70% yield.
- spacer unit (11b) (67 mg, 0.25 mmole) with (20) (0.50 g, 0.51 mmole) in the presence of potassium carbonate (0.09 g, 0.65 mmole) was performed in acetonitrile (25 ml). After work-up and recrystallization from cetonitrile, (2b) was obtained as a white powder in 65% yield. Mp: 293- 295°C.
- the tert-butyloxycarbonyl-protected spacers (15) were synthesized as described for spacer units (21), using chloroacetyl chloride (0.56 ml, 7.2 mmoles) in ethyl acetate (3 ml), and a mixture of ⁇ /-(terf-butyloxycarbonyl- 1 ,3-propanediamine (1.00 g, 5.7 mmoles) and potassium carbonate (3.2 g, 23 mmoles) in ethyl acetate (20 ml) and water (20 ml). The solution was stirred for 3 h at room temperature and the organic layer was separated and dried over magnesium sulfate.
- the alkylation was carried out in the same way as described for hexaamide biscalix[4]arenes (2), using (10) (2.00 g, 2.02 mmoles), (23d) (0.56 g, 2.23 mmoles), potassium carbonate (0.34 g, 2.42 mmoles), a catalytic amount of potassium iodide, and acetonitrile (100 ml). After evaporation of the solvent, (13d) was obtained as a foam in 58% yield, and was used as such for further reactions.
- the non-symmetric, double calix[4]arene was prepared in the same way as described for (3d), using (7) (0.25 g, 0.26 mmole) dissolved in dichloromethane (20 ml), and a solution of (14f) (0.30 g, 0.26 mmole) and triethylamine (77 ⁇ l, 1.0 mmole) in dichloromethane (10 ml). Purification gave a white solid in 68% yield. Mp: 106-108°C.
- the photophysical properties of dinuclear complexes (2b Eu 2 ) and (2b Eu ' Nd) were studied in chloroform, acetonitrile, tetrahydrofuran (THF), and methanol.
- the solubility of the complexes was low, and so the solutions contained solid particles that caused scattering.
- the luminescence spectra of the chloroform, acetonitrile, and THF solutions show the typical 5 D 0 ⁇ 7 F j transitions at 590, 615, 650, and 695 nm, after ligand-mediated excitation at 300 nm, and direct excitation at 393 nm.
- the relative intensities of the emission after 300 nm and 393 nm are dependent on the solvent and the concentration.
- the lifetime of the complex is relatively short in methanol-ct, and the solution contains a large fraction of Eu 3+ ions that have a long lifetime (1.9 ms), which may correspond to free Eu 3+ ions in solution. This is supported by the presence of a short-lived component in non-deuterated methanol (0.13 ms). In methanol, a different complex seems to exist from the one in the other solvents that were used.
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Abstract
The invention pertains to a rare-earth metal ion complex, characterized in that the complex comprises at least two complexing moieties, each of which is complexed with a rare-earth metal ion. Preferably, the rare-earth metal ion is selected from Yb3+ (ytterbium(III) ion), Er3+ (erbium(III) ion), Tb3+ (terbium(III) ion), Tm3+ (thulium(III) ion), Ho3+ (holmium(III) ion), Eu3+ (europium(III) ion), and Nd3+ (neodymium(III) ion). Preferred is the rare-earth metal ion complex which further comprises at least one sensitizer, preferably an organic sensitizer. The rare-earth metal ion complex can be used in optical devices and diagnostic kits.
Description
MULTIPLE RARE-EARTH METAL COMPLEXES
The invention pertains to rare-earth metal ion complexes and optical devices and diagnostic kits comprising the same.
Rare-earth ions are characterized by their long-lived excited state, which makes them eminently suited for optical amplification, wavelength conversion ("upconversion") and sensitive diagnostics.
Complexes of rare-earth metal ions with organic molecules having macrocyclic structures are well-known in the art. Such rare-earth metal ion complexes are excited for use in the aforementioned applications. It has now been found that the efficiency of the excitation of rare-earth ions can be further enhanced by using complexes comprising at least two complexing moieties, each of which is complexed with a rare-earth metal ion. It has further been found that when such a multiple rare-earth metal complex comprises at least one, preferably organic, sensitizer, the excitation is further improved.
The idea pertains to the use of multiple complexing units, such as calixarenes, hemispherands, polyaminocarboxyiic acids or β-diketonates, in combination with rare-earth ions of one type or of various types, with or without an attached (organic) sensitizer. The combination of rare-earth ions is of interest to enhance the excitation and/or emission possibilities of such ions (e.g., by broadening the range of absorption wavelengths) and to facilitate the use of multiple photons for excitation of these ions, with the additional possibility of wavelength conversion. The use of a sensitizer forms an additional advantage. It can be used to invoke much more efficient population of the excited state: The sensitizer has an absorption
cross section which is typically 3-4 orders of magnitude higher than that of the rare-earth ion when, for instance, organic π-π transitions are considered.
Since the lifetime of the excited state of the organic sensitizer generally is much shorter than the lifetime of the luminescent state of the rare-earth ion, multiple ions can be brought to the excited state by the same sensitizer. Therefore it is anticipated that a macromolecular unit consisting of several, preferably 2 or 3 complexing units, optionally, with attached rare-earth ions connected to at least one sensitizer, will serve as a very effective luminescent structure.
Both the same rare-earth ions and a combination of different rare-earth ions can be applied. Unless use is made of a particular property to influence the complexation behavior of the ligands, a statistical mixture of various combinations of rare-earth ions will be obtained when more than one rare-earth ion is used.
Preferred rare-earth metal ion complexes are complexes wherein the rare- earth metal ion is selected from Yb3+ (ytterbium(lll) ion), Y3+ (yttrium(lll) ion), Er3+ (erbium(lll) ion), Tb3+ (terbium(lll) ion), Tm3+ (thulium(lll) ion), Ho3+ (holmium(lll) ion), Eu3+ (europium(lll) ion), and Nd3+ (neodymium(lll) ion).
The macroscopic complex can be synthesized at a specific pH to tune the complexation properties of the ligands: for instance, the pK values of aromatic carboxylic acids, aliphatic carboxylic acids, and pyridine dicarboxylic acids will be different. Further, to activate β-diketonate an alkaline treatment is required for deprotonation.
When a specific pretreatment is applied to activate a selected part of the macroscopic structure, there may be rare-earth ion exchange.
The absorption coefficients of lanthanide ions are usually low due to their weak absorption bands as compared to, e.g., organic molecules. Yb3+, which is often used in lasers and optical amplifiers as a co-dopant, has a somewhat higher absorption coefficient than other rare-earth ions around 980 nm, where relatively inexpensive powerful lasers are available. Therefore, it can be used for excitation and subsequent transfer of its energy to another lanthanide ion which emits light in a wavelength region that is interesting for application in optical telecommunications, e.g., Er3+, which emits around 1530 nm. The application of this technique in current telecommunications networks renders energy transfer studies from one lanthanide ion to another important. Therefore, multiple complexes are required in which the lanthanide-lanthanide ion distance is relatively short (preferably <50 A). The energy transfer studies can be performed by detection of the luminescence of the acceptor lanthanide ion, or by a decrease of the lifetime of the luminescent excited state of the donor ion. The latter method is most frequently used because it is easily accessible.
Another application of multiple rare-earth ion complexes which may be of interest is in frequency (or wavelength) conversion. Examples are so-called upconverting phosphori, which are well-known in inorganic crystals, but so far have no organic counterpart. Frequency upconversion can also be realized by excited state absorption processes, due to the relatively long- living excited state of rare-earth ions. For upconversion inorganic crystals containing Y3+ and Yb3+ in combination with Er3+, Ho3+, or Tm3+ have been applied. Excitation is generally done via Yb3+in the 950-1000 nm range; particularly the wavelength region around 980 nm, where relatively inexpensive powerful laser sources are available, is suitable for excitation.
Emission wavelengths depend on the composition and structure of the inorganic crystals. Emission bands in the blue (around 400 nm), green (450-500 nm), orange (530-560 nm), and red (630-690 nm) parts of the spectrum have been reported. Application of such wavelength conversion materials is expected in two areas: (1) for optical telecommunications, when one desires to convert near-IR light (as used for high transmission long distance links) to visible light (which is transmitted most efficiently by low cost, polymer based short distance networks, e.g., in the home), and (2) for sensitive diagnostics where excitation can be done in the near-IR where no interference occurs with constituents of bodily fluids or specimens and emission is effected in the visible part of the spectrum, where the most sensitive detectors presently available have their optimum sensitivity.
Suitable complexing moieties are macrocyclic structures, in particular calix[4]arenes. Calix[4]arenes are well-known building blocks in supramolecular chemistry because of their relatively easy functionalization. They consist of four phenolic rings that are linked via methylene bridges and can adopt different conformations. The cone conformation is the most stable one. The phenolic groups are all at the same face of the molecule (lower rim) and form a cyclic array of intramolecular hydrogen bonds in this conformation. As a consequence of the vase-like structure of the cone conformation and the presence of four aromatic units, this building block is called the calix[4]arene ("calix" or "chalise" is Greek for vase). The number of linked aromatic units can vary from 4 to 8, and is indicated by the number between brackets. Calix[4]arenes have been combined with several other building blocks, like resorcinarenes, cyclodextrins, terphenyls, and porphyrins, with the intention to build larger structures. This objective can also be achieved by the covalent coupling of two or more calix[4]arenes, either by lower-lower, lower-upper, or upper-upper rim linkages. By making use of functional groups containing donor atoms that are able to coordinate
to lanthanide ions, it is possible to acquire multinuclear lanthanide ion complexes.
For energy transfer between two different lanthanide ions, biscalix[4]arenes that contain two molecular cages suitable for lanthanide ion complexation can be used. The hexaester biscalix[4]arene, for instance, can be synthesized according to McKervey et al., Anqew. Chem. Int. Ed. Engl., 1990, (29), 280 and contains a sufficient number of donor atoms. Furthermore, biscalix[4]arenes containing amide groups instead of ester functions were synthesized. The synthesis routes leading to hexaesters and hexaamides both start from p-ter--butylcalix[4]arene and consist of four reaction steps. Moreover, the combination of differently functionalized calix[4]arenes leads to non-symmetric biscalix[4]arenes that may be capable of selective complexation of different lanthanide ions.
The invention is further illustrated by the following examples.
Examples
The examples which support the present invention can be divided into three separate classes, symmetric hexaester biscalix[4]arenes (general structure 1 in Chart 1), symmetric hexaamide biscalix[4]arenes (general structure 2 in Chart 1), and asymmetric biscalix[4]arenes (general structure 3 in Chart 1).
Example 1
Synthesis of symmetric hexaester biscalix[4]arenes 1a-f (Scheme 1)
Starting materials
Starting material p-fetf-butylcalix[4]arene(4) was converted to tetraethyl ester (5) (R = ferf-butyl) (according to F. Amaud-Neu et al., J. Am. Chem. Soc, 111 , (1989) 8681 , and subsequently hydrolyzed to the triethyl ester monoacid derivative (6) (R = ferf-butyl) (according to V. Bόhmer et al., _)__ Chem. Soc, Perkin Trans. 1 , 1990, 431). Coupling of the calix[4]arene to a diamine spacer unit was performed via a slightly modified literature procedure. Triethyl ester monoacid chloride (7) (R = ferf-butyl) was obtained from (6) in refluxing oxalyl chloride, and was reacted with 0.5 mole equivalents of the appropriate diamine in dichloromethane, in the presence of triethylamine as a base. Symmetric biscalix[4]arenes (1tBu) were purified by recrystallization, mainly from a mixture of dichloromethane and methanol (95:5; v/v), and obtained in high yields (65-76%). In all cases the main product was accompanied by tetraethyl ester (5)(R = tetf-butyl). The procedure for compounds with R=H is similar to the procedure for R = ferf- butyl.
General procedure for the synthesis of hexaester biscalix!4 larenes (1a-f)
Biscalix[4]arenes (1a-f) were prepared according to a slightly modified literature procedure. Triester-monoacid 6 (R = ferf-butyl or H) was refluxed in oxalyl chloride (5 ml) for 2 hours. After evaporation of the remaining oxalyl chloride, the monoacid chloride was dissolved in dichloromethane (30 ml) under an argon atmosphere. A solution of 0.5 mole equivalents of the corresponding diamine and triethylamine in dichloromethane (12 ml) was slowly added. The reaction mixture was stirred overnight at room temperature and subsequently quenched with an aqueous acetic acid solution (5%, 25 ml). The layers were separated, and the organic layer was washed twice with water (50 ml), followed by evaporation of the solvent. The product was recrystallized from dichloromethane/methanol(95:5; v/v) unless stated otherwise.
The following compounds were prepared:
1,3-Bis{25-[(aminocarbonyl)methoxy]-5, 11, 17,23-tetrakis(1, 1 -dimethylethyl)- 26,27, 28-tris[(etho-xycarbonyl)methoxy]calix[4]arene}benzen 1tBua)
The reaction was performed using (6) (R = ferf-butyl) (0.50 g, 0.52 mmoles), 1 ,3-phenylenediamine (30 mg, 0.28 mmole), and triethylamine (0.30 ml, 2.1 mmoles). A white solid was obtained in 76% yield. Mp: 132-134°C.
1,4-Bis{25-[(aminocarbonyl)methoxy]-5, 11, 17,23-tetrakis(1, 1-dimethylethyl)- 26,27,28-tris[(etho-xycarbonyl)methoxy]calix[4]arene}benzene(1tBub)
Calix[4]arene 6 (R = ferf-butyl) (1.00 g, 1.04 mmoles) was coupled to 1 ,4- phenylenediamine (60 mg, 0.57 mmole) in the presence of triethylamine (0.60 ml, 4.2 mmoles), and ( iBub) was obtained as a white solid in 72% yield. Mp: 258-260°C.
1,3-Bis{25-[(aminocarbonyl)methoxy]-5, 11, 17,23-tetrakis(1, 1-dimethylethyl)- 26,27, 28-tris[(etho-xycarbonyl)methoxy]calix[4]arene}propan^1tBud)
Compound (6) (R = ferf-butyl) (0.50 g, 0.52 mmole), 1 ,3-diaminopropane(22 μl, 0.26 mmole), and triethylamine (0.30 ml, 2.1 mmoles) were used as reagents, and recrystallization yielded (1tBu d) in 76% yield. Mp: 152-153°C.
1,4-Bis{25-[(aminocarbonyl)methoxy]-5, 11, 17,23-tetrakis(1, 1 -dimethylethyl)- 26,27, 28-tris[(etho-xycarbonyl)methoxy]calix[4]arene}butan tBue)
Coupling of (6) (R = ferf-butyl) (1.00 g, 1.04 mmoles) and 1 ,4-diaminobutane (60 μl, 0.57 mmole), in the presence of triethylamine (0.60 ml, 4.2 mmoles),
yielded (1tBue) after recrystallization from a mixture of chloroform and acetonitrile(4:1) in 65% yield. Mp: 201-203°C.
1,6-Bis{25-[(aminocarbonyl)methoxy]-5, 11, 17, 23-tetrakis(1 , 1-dimethylethyl)- 26,27, 28-t s[(etho-xycarbonyl)methoxy]calix[4]arene}hexane(1tBuf)
The double calix[4]arene(1tBuf) was obtained in 67% yield from (15) (R = ferf- butyl) (1.49 g, 1.45 mmoles), 1 ,6-diaminohexane (84 μl, 0.73 mmole), and triethylamine (0.90 ml, 6.3 mmoles). Mp: 134-136°C.
7 , 3-Bis{25-[(aminocarbonyl)methoxy]-26, 27,28- tris[(ethoxycarbonyl)methoxy]calix[4]arene}-benzene(1Ha)
The reaction of (6) (R = H) (0.51 g, 0.69 mmole) and 1 ,3-phenylenediamine (40 mg, 0.37 mmole) in the presence of triethylamine (0.40 ml, 2.8 mmoles) gave white crystals in 51% yield. Mp: 137-140°C.
1,4-Bis{25-[(aminocarbonyl)methoxy]-26,27,28- tris[(ethoxycarbonyl)methoxy]calix[4]arene}-benzene(1Hb)
Compound (1Hb) was obtained as a white solid in 76% yield by reaction of (6) (R = H) (0.50 g, 0.67 mmole), 1 ,4-phenylenediamine (40 mg, 0.37 mmole), and triethylamine (0.40 ml, 2.8 mmoles). Mp: 183-185°C.
1,2-Bis{25-[(aminocarbonyl)methoxy]-26,27,28- trisKethoxycarbonyljmethoxylcalixfflarenej-ethaneiltfi)
The coupling was performed using (6) (R = H) (0.50 g, 0.67 mmole), 1 ,2- diaminoethane (22 μl, 0.33 mmole), and triethylamine (0.40 ml, 2.8 mmoles). Recrystallization led to (1Hc) in 59% yield. Mp: 175-177°C.
1,3-Bis{25-[(aminocarbonyl)methoxy]-26,27,28- tris ethoxycarbonytymethoxyJcalixfflarene -propaneflHd)
Reaction of (6) (R = H) (0.50 g, 0.67 mmole) with 1 ,3-diaminopropane(27 μl, 0.32 mmole) in the presence of triethylamine (0.40 ml, 2.8 mmoles) gave (1Hd) as a white solid in 61% yield. Mp: 173-174°C.
Example 2
Synthesis of symmetric hexa-amide biscalix[4]arenes 2b,e,f (Scheme 2)
General description of the synthesis
The synthesis route leading to biscalix[4]arene hexaamides starts with the monoprotection of p-ferf-butylcalix[4]arene (4) with 3-bromo-1-propene in N.N'-dimethylformamide using CsF as a base. The monoprotection was performed via a slightly modified literature procedure (L.C. Groenen, Conformational Properties of Calixarenes, PhD Thesis, University of Twente 1992). Calix[4]arene (8) was trialkylated with 2-chloro-N,N'-diethylacetamide in acetonitrile in the presence of a catalytic amount of potassium iodide and potassium carbonate as a base. After work up and recrystallization from acetonitrile, compound (9) was obtained in 57% yield. Deprotection of (10) was performed with Pd(PPh3)4 and triethylamine HCOOH in a mixture of ethanol and water (according to H. Hey et al., Anqew. Chem. Int. Ed. Engl., 12, (1973) 928).
The product was purified by recrystallization from acetonitrile and obtained in
77% yield. Three different spacer units (11b), (11e), and (11f) were prepared by reaction of the corresponding diamine with chloroacetyl chloride in a
mixture of ethyl acetate and water, using potassium carbonate as a base. These spacers were obtained in 70-79% yield, and characterized by 1H NMR spectroscopy. Finally, the hexaamides (12b,e,f) were prepared via alkylation of (10) with 0.5 mole equivalents of spacer unit 11), a catalytic amount of potassium iodide, and potassium carbonate as a base. Acidic work-up and recrystallization resulted in biscalix[4]arenes (12b,e,f) in > 64% yield, which were pure according to elemental analysis. In all cases, the 2:1 calix[4]arene:spacer ratio was obvious from the integrals in the 1H NMR spectra.
Synthesis of intermediate compounds
25,26,27-Tris[(N,N-diethylaminocarbonyl)methoxy]-5, 11, 17,23-tetrakis(1 , 1- dimethylethyl)-28-(2-propenyloxy)calix[4]arene(9)
A mixture of monoallylated calix[4]arene (8) (5.00 g, 7.26 mmoles), potassium carbonate (5.0 g, 36 mmoles), a catalytic amount of potassium iodide, and 2-chloro-Λ/,Λ/-diethylacetamide (2.70 ml, 26.2 mmoles) in acetonitrile (300 ml) was refluxed overnight. After cooling to room temperature, the salts were filtered off and the acetonitrile was evaporated. The crude product was taken up in dichloromethane (200 ml), washed twice with a saturated aqueous solution of ammonium chloride (250 ml) and twice with water (250 ml), and was subsequently dried over magnesium sulfate. Recrystallization from acetonitrile gave pure (9) in 57% yield. Mp: 93-95°C.
25,26,27-Tris[(N,N-diethylaminocarbonyl)methoxy]-5, 11, 17,23-tetrakis(1 , 1- dimethylethyl)calix-[4]arene (10)
A mixture of (9) (3.00 g, 2.92 mmoles), triethylamine HCOOH (0.52 g, 3.50 mmoles), Pd(PPh3)4 (0.25 g, 0.22 mmole) in ethanol (60 ml) and water (12
ml) was refluxed for 2 h. After cooling to room temperature, the solvents were evaporated and the crude product was taken up in chloroform (50 ml). The organic layer was washed twice with a saturated aqueous solution of ammonium chloride (50 ml) and twice with water (50 ml). After drying over magnesium sulfate and evaporation of the solvent, the crude product was recrystallized from acetonitrile (20 ml). Compound (10) was obtained as a white powder in 77% yield. Mp: 225-227°C.
General procedure for the preparation of spacer units (11)
A solution of chloroacetyl chloride in ethyl acetate (20 ml) was added dropwiseto a mixture of the appropriate diamine and potassium carbonate in ethyl acetate and water. The reaction mixture was stirred overnight, and the precipitate was filtered off. The product was washed with cold ethyl acetate and dried under vacuum. Compounds (11 ) were used as such.
N, N'-Bis(chloromethyl)carbonyl-1,4-diaminobenzene (11b)
The reaction was performed using chloroacetyl chloride (8.63 ml, 0.11 mole) 1 ,4-phenylenediamine (4.05 g, 37 mmoles), and potassium carbonate (25.6 g, 0.18 mmole) in a mixture of ethyl acetate (200 ml) and water (200 ml), and gave product (11b) in 79%.
N, N'-Bis(chloromethyl)carbonyl- 1 , 4-diaminobutane (11e)
Spacer (11e) was synthesized from chloroacetyl chloride (5.28 ml, 68 mmoles) 1 ,4-diaminobutane (2.28 ml, 22.7 mmoles), and potassium carbonate (14.5 g, 0.10 mole) in a mixture of ethyl acetate (150 ml) and water (150 ml), and was obtained in 72% yield.
N,N'-Bis(chloromethyl)carbonyl-1 ,6-diaminobutane(11f)
The reaction was carried out using chloroacetyl chloride (4.28 ml, 55 mmoles), 1 ,6-diaminohexane (2.00 g, 17.2 mmole), and potassium carbonate (10.5 g, 76 mmole) in ethyl acetate (60 ml) and water (60 ml), leading to (11f) in 70% yield.
General procedure for the preparation of hexaamide biscalix[41arenes 2b, e, f
A mixture of (10), potassium carbonate, a catalytic amount of potassium iodide, and 0.5 mole equivalents of (11) in acetonitrile was refluxed for 16 hours. After cooling to room temperature, the solvent was evaporated and the crude product was taken up in dichloromethane (50 ml). The resulting mixture was washed with a saturated aqueous ammonium chloride solution (50 ml) and subsequently with water (50 ml). Compounds (2b,e,f) were dried over magnesium sulfate, and the solvent was evaporated.
The following compounds were prepared:
1 , 4-Bis{25-[(aminocarbonyl)methoxy]-26, 27, 28-ths[(N, N- diethylaminocarbonyl)methoxy]-5, 11,-17,23-tetrakis(1, 1- dimethylethyl)calix[4]arene}benzene (2b)
The reaction of spacer unit (11b) (67 mg, 0.25 mmole) with (20) (0.50 g, 0.51 mmole) in the presence of potassium carbonate (0.09 g, 0.65 mmole) was performed in acetonitrile (25 ml). After work-up and recrystallization from cetonitrile, (2b) was obtained as a white powder in 65% yield. Mp: 293- 295°C.
1,4-Bis{25-[(aminocarbonyl)methoxy]-26, 27, 28-tris[(N, N- diethylaminocarbonyl)methoxy]-5, 11,-17,23-tetrakis(1, 1- dimethylethyl)calix[4]arene}butane (2e)
The reaction was performed using (11e) (68 mg, 0.28 mmole), 20 (0.56 g, 0.57 mmole), and potassium carbonate (83 mg, 0.68 mmole) in acetonitrile (25 ml). Recrystallization from a mixture of acetonitrile and dichloromethane (20:1) gave (22e) as a white powder in 64% yield. Mp: 263-265°C.
7 , 6-Bis{25-[(aminocarbonyl)methoxy]-26, 27, 28-tris[(N, N- diethylaminocarbonyl)methoxy]-5, 11,-17,23-tetrakis(1, 1- dimethylethyl)calix[4]arene}hexane(2f)
The coupling was carried out using (11f) (6.9 mg, 2.9 mmoles), 20 (51 mg, 51 mmoles), potassium carbonate (7.5 mg, 54 mmoles), and acetonitrile (15 ml). Recrystallization from methanol gave (2f) as a white powder in 68% yield. Mp: 237-239°C.
Example 3
Synthesis of asymmetric biscalix[4]arenes 3b,e,f (Scheme 3)
General description of the synthesis
By combination of triethyl ester monoacid chloride (7) (R = ferf-butyl) and tris(diethyl)acetamide (12) functionalized with a primary amine, non- symmetric biscalix[4]arenes were prepared. For this synthesis, the tert- butyloxycarbonyl-protectedspacers (15d) and (15f) were synthesized, in the same way as spacer units (11), from a mono-tert-butyloxycarbonyl-protected diamine and chloroacetyl chloride in a 1 :1 ratio. The products were subsequently purified by flash column chromatography.
Spacers (15) were used in the alkylation of (12). This alkylation reaction was carried out in a 1 :1 ratio, as described before.
Deprotection of (13), leading to the primary amine was performed by passing hydrochloric acid gas through a solution of (13) in dichloromethane. The products were obtained in nearly quantitative yields after evaporation of the solvent and purification by trituration with acetonitrile. The amines were coupled in a 1 :1 fashion to monoacid chloride derivative (7), using the same conditions as were employed for the formation of hexaesters. These non- symmetric biscalix[4]arenes were recrystallized from dichloromethane and methanol. Moreover, derivative (3d), which is linked via the shorter spacer, required also Sephadex column chromatography to remove the tetraester (4) formed as side product.
Synthesis of intermediate compounds
N-[(Chloromethyl)carbonyl]-N'-[(1 , 1 -dimethylethoxy)carbonyl]-1 ,3- diaminopropane (15d)
The tert-butyloxycarbonyl-protected spacers (15) were synthesized as described for spacer units (21), using chloroacetyl chloride (0.56 ml, 7.2 mmoles) in ethyl acetate (3 ml), and a mixture of Λ/-(terf-butyloxycarbonyl- 1 ,3-propanediamine (1.00 g, 5.7 mmoles) and potassium carbonate (3.2 g, 23 mmoles) in ethyl acetate (20 ml) and water (20 ml). The solution was stirred for 3 h at room temperature and the organic layer was separated and dried over magnesium sulfate. After purification by column chromatography (3% methanol in dichloromethane), (15d) was obtained as a yellow powder in 82% yield.
N-[(Chloromethyl)carbonyl]-N'-[(1, 1-dimethylethoxy)carbonyl]-1,6- diaminohexane (15f)
Compound (15f) was prepared in the same way as described for (15d), using a solution of chloroacetyl chloride (1.55 ml, 20 mmoles) in ethyl acetate (8 ml), and a mixture of Λ/-tert-butyloxycarbonyl-1 ,6-hexanediamine(4.00 g, 16 mmoles) and potassium carbonate (8.88 g, 64 mmoles) in ethyl acetate (50 ml) and water (50 ml). Column chromatography (3% methanol in dichloromethane) gave (15f) in 75% yield.
25,26,27-Tris[(N,N-diethylaminocarbonyl)methoxy]-28-[(1, 1- dimethylethoxy)carbonyl]-1-amino-propyl-3-[(aminocarbonyl)methoxy]- 5, 11, 17,23-tetrakis(1, 1 -dimethylethyl-i:alix[4]arene (13d)
The alkylation was carried out in the same way as described for hexaamide biscalix[4]arenes (2), using (10) (2.00 g, 2.02 mmoles), (23d) (0.56 g, 2.23 mmoles), potassium carbonate (0.34 g, 2.42 mmoles), a catalytic amount of potassium iodide, and acetonitrile (100 ml). After evaporation of the solvent, (13d) was obtained as a foam in 58% yield, and was used as such for further reactions.
25, 26, 27- Tris[(N, N-diethylaminocarbonyl)methoxy]-28-[(1, 1- dimethylethoxy)carbonyl]-1-amino-hexyl-6-[(aminocarbonyl)methoxy]- 5, 11, 17,23-tetrakis(1, 1 -dimethylethyl)calix[4]arene(13f)
The alkylation was carried out in the same way as described for biscalix[4]arenes (2), using (10) (2.00 g, 2.02 mmoles), (15f) (0.65 g, 2.22 mmole), potassium carbonate (0.34 g, 2.42 mmoles), a catalytic amount of potassium iodide, and acetonitrile (100 ml). Compound (13f) was obtained as a foam in 62% yield and was used for further reactions without purification.
25-[1-Aminopropyl-3-(aminocarbonyl)methoxy]-26,27, 28-tris[(N, N'- diethylaminocarbonyl)me-thoxy]-5, 11, 17,23-tetrakis(1 , 1- dimethylethyl)calix[4]arene(14d)
Hydrochloric acid gas was led through a solution of (13d) (0.94 g, 0.78 mmole) dissolved in dichloromethane (45 ml) for 30 minutes. The solvent was evaporated, and the white residue was triturated with acetonitrile to give compound (14d) in nearly quantitative yield. Mp: 127-129°C.
25-[1-Aminohexyl-6-(aminocarbonyl)methoxy]-26,27,28-tris[(N,N'- diethylaminocarbonyl)me-thoxy]-5, 11, 17,23-tetrakis(1 , 1- dimethylethyl)calix[4]arene(14f)
Compound (14f) was obtained in the same way as described for (14d), using hydrochloric acid gas, and (13f) (1.55 g, 1.25 mmoles) dissolved in dichloromethane (35 ml). After trituration with acetonitrile, a white powder was obtained in nearly quantitative yield. Mp: 133-134°C.
The following compounds were prepared:
1 -{25-[(Aminocarbonyl)methoxy]-26, 27, 28-tris[(N, N- diethylaminocarbonyl)methoxy]-5, 11, 17,23-tetrakis(1 , 1- dimethylethyl)calix[4]arene}-3-{25-[(aminocarbonyl)methoxy]-5, 11, 17,23- tetra-kis(1, 1-dimethylethyl)-26,27, 28- tris[(ethoxycarbonyl)methoxy]calix[4]aren ropane (3d)
The coupling reaction was performed in the same way as described for hexaesters (1), using (7) (0.25 g, 0.26 mmole) dissolved in dichloromethane
(20 ml) and a solution of (14d) (0.29 g, 0.26 mmole) and triethylamine (77 μl, 1.0 mmole) in dichloromethane (10 ml). The product was purified by
recrystallization from dichloromethane/methanol, followed by Sephadex (LH20/methanol) column chromotography. Compound (3d) was obtained as a white solid in 70% yield. Mp: 120-122°C.
1-{25-[(Aminocarbonyl)methoxy]-26,27, 28-tris[(N, N- diethylaminocarbonyl)methoxy]-5, 11, 17,23-tetrakis(1 , 1- dimethylethyl)calix[4]arene}-6-{25-[(aminocarbonyl)methoxy]-5, 11, 17,23- tetra-kis(1, 1-dimethylethyl)-26,27,28- tris[(ethoxycarbonyl)methoxy]calix[4]arenej}ιexane (3f)
The non-symmetric, double calix[4]arene was prepared in the same way as described for (3d), using (7) (0.25 g, 0.26 mmole) dissolved in dichloromethane (20 ml), and a solution of (14f) (0.30 g, 0.26 mmole) and triethylamine (77 μl, 1.0 mmole) in dichloromethane (10 ml). Purification gave a white solid in 68% yield. Mp: 106-108°C.
Example 4
Lanthanide ion complexation
General methods
Different methods were used to complex Eu3+ ions inside biscalix[4]arenes. In all cases, biscalix[4]arenewas dissolved in acetone, acetonitrile, or either of these solvents in combination with dichloromethane. Subsequently, two equivalents of a Eu(NO3)36H2O were added, followed by stirring overnight either at room or at reflux temperature. The white solids were collected either by precipitation or by evaporation of the solvents. Luminescence studies revealed that hexaester (1tBub) does not complex any Eu3+ ions, since stirring in acetone at room temperature only resulted in "free" Eu3+ ions in solution.
Moreover, the precipitate that was collected after refluxing overnight in acetonitrile did not show any detectable luminescence, indicating the absence of Eu3+ ions. On the other hand, all preparation methods that were carried out to prepare Eu3+ complexes of the hexaamide (2b) were successful. However, the highest yield was achieved in refluxing acetonitrile. In addition, biscalix[4]arene (2b) was complexed with one equivalent of Eu3+ and one equivalent of Nd3+ by the same method. The biscalix[4]arene showed a slight selectivity for Nd3+ as was revealed by the ionic distribution of 1 :1.3 determined by X-ray fluorescence.
Eu .3+ Complexation
( iEyb : 1) dissolve calix[4]arene in acetone, add two equivalents of Eu(NO3)3 in acetone (stock solution), stir overnight, evaporate solvent, dry under vacuum.
2) dissolve calix[4]arene in dichloromethane/acetonitrile (1 :1), add two equivalents of Eu(NO3)3 in acetonitrile, reflux overnight, collect precipitate, dry under vacuum.
(2b): 1) dissolve calix[4]arene in acetone/dichloromethane (3:1), add two equivalents of Eu(NO3)3 in acetone, stir overnight, a) evaporate solvent or b) remove solvent with pipette, dry under vacuum.
2) as 1) reflux overnight, collect precipitate, dry under vacuum. as 2) in acetonitrile. , 4-Bis[25-aminocarbonyl-26, 27, 28-tris[(N, N- diethylaminocarbonyl)methoxy]-5, 11, 17,23-tetrakis(1 , 1- dimethylethyl)calix[4]arene]benzenedieuropium(lll)hexanitrate(-)
(2b2[Eu(N03)3l) A homodinuclear complex (2b2[Eu(NO3)3]) was prepared by method 3, using biscalix[4]arene (22b) (0.108 g, 49.9 μmoles) and a stock solution of 19.9 mM Eu(NO3)36H2O (5.02 ml, 99.9 μmoles) in acetonitrile.
Mp: >300°C.
1 , 4-Bis[25-aminocarbonyl-26, 27, 28-tris[(N, N-diethylaminocarbonyl)methoxy]- 5, 11, 17, 23-tetrakis-(1 , 1-dimethylethyl)calix[4]arene]benzene europium(lll) neodymium(lll) hexanitrate(-) (2b[Eu(N0^3JlNd(N0^3j) A heterodinuclear complex (2b [Eu(NO3)3] [Nd(NO3)3]) was prepared by method 3, using biscalix[4]arene22b (58.2 mg, 26.9 μmoles) stock solutions of 6.14 mM Eu(NO3)36H2O (4.39 ml, 26.9 μmoles), and 6.71 mM Nd(NO3)35H2O (4.01 ml, 26.9 μmoles) in acetonitrile. Mp: >300°C.
Example 5
Photophysical studies
The photophysical properties of dinuclear complexes (2b Eu2) and (2b Eu'Nd) were studied in chloroform, acetonitrile, tetrahydrofuran (THF), and methanol. The solubility of the complexes was low, and so the solutions contained solid particles that caused scattering. The luminescence spectra of the chloroform, acetonitrile, and THF solutions show the typical 5D0 → 7Fj transitions at 590, 615, 650, and 695 nm, after ligand-mediated excitation at 300 nm, and direct excitation at 393 nm. The relative intensities of the emission after 300 nm and 393 nm are dependent on the solvent and the concentration. Generally, direct Eu3+ excitation is more efficient than ligand- mediated excitation, which might be a result of the large distance between the aromatic sensitizing units of the calix[4]arene and the Eu3+ ion. On the other hand, the complexes behave completely differently in methanol, as is indicated by the red shift of the most intense emission band to 624 nm. This emission band has a shoulder at 614 nm, at which the peak maximum is usually positioned. Furthermore, two additional emission bands are present at 570 and 600 nm, whereas the band at 580 nm is broadened. The band around 680 nm is also red shifted in methanol-d4. The deviating Eu3+
emission, which was not observed for other Eu3+ complexes that were studied, is very sensitive to small amounts of water and can disappear completely, resulting in the usually observed Eu3+ luminescence.
The presence of Nd3+ in (2b Eu Nd) was obvious from the typical Nd3+ emission band at 1060 nm that was observed after ligand-mediated excitation. Moreover, luminescence lifetime measurements were performed with the same solutions, and the results are reported in the Table. The lifetimes of (2b Eu2) dissolved in chloroform and acetonitrile are equal within the experimental error, whereas the Eu3+ luminescence is deactivated slightly more efficiently in THF. Upon addition of water, the Eu3+ luminescence is extinguished in both acetonitrile and THF. The lifetime of the complex is relatively short in methanol-ct,, and the solution contains a large fraction of Eu3+ ions that have a long lifetime (1.9 ms), which may correspond to free Eu3+ ions in solution. This is supported by the presence of a short-lived component in non-deuterated methanol (0.13 ms). In methanol, a different complex seems to exist from the one in the other solvents that were used.
The most striking feature of the results reported in the Table is the lifetime decrease of the Eu3+ luminescent state after excitation at 300 nm when Nd3+ is present. Based on literature examples, it was expected that the decay curve would be bi-exponential, since the composition of the complex is, in principle, statistical. However, only a mono-exponential decay curve was observed in all solvents. A mixture of the homo- and heterodinuclear complexes also decayed mono-exponentially, the lifetime corresponding to that of the homodinuclear complex. The relative lifetime decrease is most pronounced in acetonitrile and the E values given in Table 1 are equal to the efficiency of energy transfer if this is the only additional deactivation pathway compared to the homodinuclear complex.
Table 1 Lifetimes (in ms) of the D0 state of (2bEu2) and (2bEu Nd) in different solvents,
after excitation at 300 nm and detection at 614 nm.a
a) Data fitting of the decay curves all show two components, one corresponding to the complex and the other to solid particles present (« 0.10 ms); b) relative decrease of the lifetime; c) detected at 624 nm; d) contains a large fraction with a lifetime of 2.5 ms; e) contains a large fraction with a lifetime of 0.1 ms; ή signal too weak.
Claims
1. A rare-earth metal ion complex, characterized in that the complex comprises at least two complexing moieties, each of which is complexed with a rare-earth metal ion.
2. The rare-earth metal ion complex of claim 1 wherein the complexing moiety has a macrocyclic structure.
3. The rare-earth metal ion complex of claim 1 or 2 wherein at least two different rare-earth metal ion are complexed.
4. The rare-earth metal ion complex of any one of claims 1-3, comprising 2 or 3 complexing moieties.
5. The rare-earth metal ion complex of any one of claims 1-4 wherein the rare-earth metal ion is selected from Yb3+ (ytterbium(lll) ion), Y3+ (yttrium(lll) ion), Er3+ (erbium(lll) ion), Tb3+ (terbium(lll) ion), Tm3+ (thulium(lll) ion), Ho3+ (holmium(lll) ion), Eu3+ (europium(lll) ion), and Nd3+ (neodymium(lll) ion).
6. The rare-earth metal ion complex of any one of claims 1-5 wherein the complex further comprises at least one sensitizer, preferably an organic sensitizer.
7. An optical device comprising the rare-earth metal ion complex of any one of claims 1-7.
8. A diagnostic kit comprising the rare-earth metal ion complex of any one of claims 1-7.
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CN108329336A (en) * | 2018-03-15 | 2018-07-27 | 中国科学院苏州生物医学工程技术研究所 | The metal complex and its synthetic method of double cup [4] arene derivatives and application |
CN108395372A (en) * | 2018-03-15 | 2018-08-14 | 中国科学院苏州生物医学工程技术研究所 | The metal complex and its synthetic method of double cup [4] arene derivatives and application |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2008510749A (en) * | 2004-08-24 | 2008-04-10 | バイエル・テクノロジー・サービシーズ・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング | Specific calixarene, its production method and its use |
JP4906726B2 (en) * | 2004-08-24 | 2012-03-28 | バイエル・テクノロジー・サービシーズ・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング | Specific calixarene, its production method and its use |
CN108329336A (en) * | 2018-03-15 | 2018-07-27 | 中国科学院苏州生物医学工程技术研究所 | The metal complex and its synthetic method of double cup [4] arene derivatives and application |
CN108395372A (en) * | 2018-03-15 | 2018-08-14 | 中国科学院苏州生物医学工程技术研究所 | The metal complex and its synthetic method of double cup [4] arene derivatives and application |
CN108395372B (en) * | 2018-03-15 | 2019-09-10 | 中国科学院苏州生物医学工程技术研究所 | The metal complex and its synthetic method of double cup [4] arene derivatives and application |
CN108329336B (en) * | 2018-03-15 | 2020-06-05 | 中国科学院苏州生物医学工程技术研究所 | Metal complex of dicapryl [4] arene derivative and synthetic method and application thereof |
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