WO1998036053A3 - Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages - Google Patents

Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages Download PDF

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Publication number
WO1998036053A3
WO1998036053A3 PCT/US1998/002752 US9802752W WO9836053A3 WO 1998036053 A3 WO1998036053 A3 WO 1998036053A3 US 9802752 W US9802752 W US 9802752W WO 9836053 A3 WO9836053 A3 WO 9836053A3
Authority
WO
WIPO (PCT)
Prior art keywords
recombinant dna
dna sequences
purified proteins
processes
expression
Prior art date
Application number
PCT/US1998/002752
Other languages
French (fr)
Other versions
WO1998036053A2 (en
Inventor
John I Stiles
Istefo Moisyadi
Kabi R Neupane
Original Assignee
Univ Hawaii
John I Stiles
Istefo Moisyadi
Kabi R Neupane
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Hawaii, John I Stiles, Istefo Moisyadi, Kabi R Neupane filed Critical Univ Hawaii
Priority to PCT/US1998/002752 priority Critical patent/WO1998036053A2/en
Priority to AU63247/98A priority patent/AU6324798A/en
Publication of WO1998036053A2 publication Critical patent/WO1998036053A2/en
Publication of WO1998036053A3 publication Critical patent/WO1998036053A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Tea And Coffee (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Purified proteins, DNA sequences that code on expression therefor and recombinant DNA molecules, including hosts transformed therewith, for transforming coffee plants to suppress the expression of caffeine. The DNA sequences and recombinant DNA molecules are characterized in that they code on expression for an enzyme in the pathway for caffeine synthesis in coffee. Coffee plants transformed with DNA molecules that code on transcription for mRNA that is antisense to mRNA that codes on expression for at least one enzyme in the pathway for caffeine biosynthesis.
PCT/US1998/002752 1998-02-13 1998-02-13 Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages WO1998036053A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PCT/US1998/002752 WO1998036053A2 (en) 1998-02-13 1998-02-13 Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages
AU63247/98A AU6324798A (en) 1998-02-13 1998-02-13 Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US1998/002752 WO1998036053A2 (en) 1998-02-13 1998-02-13 Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages

Publications (2)

Publication Number Publication Date
WO1998036053A2 WO1998036053A2 (en) 1998-08-20
WO1998036053A3 true WO1998036053A3 (en) 1999-12-09

Family

ID=22266381

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/002752 WO1998036053A2 (en) 1998-02-13 1998-02-13 Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages

Country Status (2)

Country Link
AU (1) AU6324798A (en)
WO (1) WO1998036053A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6075184A (en) * 1996-03-26 2000-06-13 University Of Hawaii Purified proteins, recombinant DNA sequences and processes for producing caffeine free beverages
US6930227B1 (en) * 1999-05-26 2005-08-16 Mitsui Chemicals, Inc. Camellia sinensis gene encoding a caffeine synthesis associated n-methyl transferase with 7-methylxanthine n3 methyl transferase, theobromine n1 methyl transferase, and paraxanthine n3 methyl transferase activities and use thereof
EP1256629A1 (en) * 2001-05-11 2002-11-13 Société des Produits Nestlé S.A. Leaf specifc gene promoter of coffee
GB201807192D0 (en) 2018-05-01 2018-06-13 Tropic Biosciences Uk Ltd Compositions and methods for reducing caffeine content in coffee beans
IL305071A (en) * 2021-03-02 2023-10-01 Betterseeds Ltd Domestication of a legume plant

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5334529A (en) * 1989-06-27 1994-08-02 Escagenetics Corporation Stably transformed coffee plant cells and plantlets
WO1997035960A1 (en) * 1996-03-26 1997-10-02 University Of Hawaii Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5334529A (en) * 1989-06-27 1994-08-02 Escagenetics Corporation Stably transformed coffee plant cells and plantlets
WO1997035960A1 (en) * 1996-03-26 1997-10-02 University Of Hawaii Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KATO M ET AL: "CAFFEINE BIOSYNTHESIS IN YOUNG LEAVES OF CAMELLIA SINENSIS: IN VITRO STUDIES ON NU-METHYLTRANSFERASE ACTIVITY INVOLVED IN THE CONVERSION OF XANTHOSINE TO CAFFEINE", PHYSIOLOGIA PLANTARUM, vol. 98, no. 3, 1 January 1996 (1996-01-01), pages 629 - 636, XP002071639, ISSN: 0031-9317 *
SCHULTHESS B H ET AL: "CAFFEINE BIOSYNTHESIS STARTS WITH THE METABOLICALLY CHANNELLED FORMATION OF 7-METHYL-XMP-A NEW HYPOTHESIS", PHYTOCHEMISTRY, vol. 41, no. 1, 1 January 1996 (1996-01-01), pages 169 - 175, XP002071640, ISSN: 0031-9422 *
SUZUKI T ET AL: "PURINE AND PURINE ALKALOID METABOLISM IN CAMELLIA AND COFFEA PLANTS", PHYTOCHEMISTRY, vol. 31, no. 8, 1 January 1992 (1992-01-01), pages 2575 - 2584, XP002071641, ISSN: 0031-9422 *

Also Published As

Publication number Publication date
WO1998036053A2 (en) 1998-08-20
AU6324798A (en) 1998-09-08

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