WO1998035058A3 - Procede ameliore de detection et de quantification de molecules d'acides nucleiques - Google Patents

Procede ameliore de detection et de quantification de molecules d'acides nucleiques Download PDF

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Publication number
WO1998035058A3
WO1998035058A3 PCT/US1998/001471 US9801471W WO9835058A3 WO 1998035058 A3 WO1998035058 A3 WO 1998035058A3 US 9801471 W US9801471 W US 9801471W WO 9835058 A3 WO9835058 A3 WO 9835058A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid molecules
quantification
detection
improved process
Prior art date
Application number
PCT/US1998/001471
Other languages
English (en)
Other versions
WO1998035058A2 (fr
Inventor
James D Thompson
Original Assignee
Ribozyme Pharm Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ribozyme Pharm Inc filed Critical Ribozyme Pharm Inc
Priority to AU63173/98A priority Critical patent/AU6317398A/en
Publication of WO1998035058A2 publication Critical patent/WO1998035058A2/fr
Publication of WO1998035058A3 publication Critical patent/WO1998035058A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Abstract

L'invention concerne un procédé de mesure des quantités relatives de deux molécules d'acides nucléiques différentes ou davantage dans un système biologique au moyen d'une amplification en chaîne par polymérase multiplex compétitive.
PCT/US1998/001471 1997-02-07 1998-01-27 Procede ameliore de detection et de quantification de molecules d'acides nucleiques WO1998035058A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU63173/98A AU6317398A (en) 1997-02-07 1998-01-27 Improved process for detection and quantification of nucleic acid molecules

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US3784197P 1997-02-07 1997-02-07
US60/037,841 1997-02-07
US99373197A 1997-12-18 1997-12-18
US08/993,731 1997-12-18

Publications (2)

Publication Number Publication Date
WO1998035058A2 WO1998035058A2 (fr) 1998-08-13
WO1998035058A3 true WO1998035058A3 (fr) 1998-09-17

Family

ID=26714548

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/001471 WO1998035058A2 (fr) 1997-02-07 1998-01-27 Procede ameliore de detection et de quantification de molecules d'acides nucleiques

Country Status (1)

Country Link
WO (1) WO1998035058A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6345700A (en) * 1999-07-14 2001-02-05 Packard Bioscience Company Derivative nucleic acids and uses thereof
US6964847B1 (en) 1999-07-14 2005-11-15 Packard Biosciences Company Derivative nucleic acids and uses thereof
WO2002048352A1 (fr) * 2000-12-12 2002-06-20 Aisin Seiki Kabushiki Kaisha Methode d'analyse d'expression genique
EP1468117A2 (fr) * 2002-01-15 2004-10-20 MATFORSK, Norwegian Food Research Institute Techniques d'amplification d'acide nucleique
FI20040723A0 (fi) 2004-05-26 2004-05-26 Orpana Aarne Menetelmä mRNA:n ilmentymistasojen kvantitatiiviseksi ja/tai suhteelliseksi mittaamiseksi pienissä biologisissa näytteissä
US7575863B2 (en) 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
US20060057595A1 (en) * 2004-09-16 2006-03-16 Applera Corporation Compositions, methods, and kits for identifying and quantitating small RNA molecules

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994023023A1 (fr) * 1993-04-06 1994-10-13 University Of Rochester Procede pour la mesure quantitative d'une expression de gene utilisant une transcription inverse et une amplification enzymatique du genome, multiplexe et competitive
EP0628640A1 (fr) * 1993-06-04 1994-12-14 Becton, Dickinson and Company Amplification simultanée de cibles multiples

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994023023A1 (fr) * 1993-04-06 1994-10-13 University Of Rochester Procede pour la mesure quantitative d'une expression de gene utilisant une transcription inverse et une amplification enzymatique du genome, multiplexe et competitive
EP0628640A1 (fr) * 1993-06-04 1994-12-14 Becton, Dickinson and Company Amplification simultanée de cibles multiples

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BABU ET AL.: "LIMITATIONS AND MODIFICATIONS OF QUANTITATIVE POLYMERASE CHAN REACTION", J. IMMUN. METH., vol. 165, 1993, pages 207 - 216, XP002071877 *
CELI F S ET AL: "A RAPID AND VERSATILE METHOD TO SYNTHESIZE INTERNAL STANDARDS FOR COMPETITIVE PCR", NUCLEIC ACIDS RESEARCH, vol. 21, no. 4, 25 February 1993 (1993-02-25), pages 1047, XP000349930 *
FERRE F ET AL: "QUANTITATIVE PCR: AN OVERVIEW", 1994, POLYMERASE CHAIN REACTION, PAGE(S) 67 - 88, MULLIS K B;FERRE F; GIBBS R A, XP002017884 *
KELLER AND MANAK: "DNA PROBES", 1994, STOCKTON PRESS, US, NY, XP002071878, 208660 *
REISCHL U ET AL: "QUANTITATIVE PCR A SURVEY OF THE PRESENT TECHNOLOGY", MOLECULAR BIOTECHNOLOGY, vol. 3, 1995, pages 55 - 71, XP000600241 *
SECCHIERO P ET AL: "QUANTITATIVE PCR FOR HUMAN HERPESVIRUSES 6 AND 7", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 33, no. 8, 1 August 1995 (1995-08-01), pages 2124 - 2130, XP000564243 *
SIEBERT AND LARRICK: "COMPETITVE PCR", NATURE, vol. 359, 1992, pages 557 - 558, XP002071876 *
ZIMMERMANN K ET AL: "TECHNICAL ASPECTS OF QUANTITATIVE COMPETITIVE PCR", BIOTECHNIQUES, vol. 21, no. 2, 1 August 1996 (1996-08-01), pages 268 - 270, 272, 274 - 279, XP000597689 *

Also Published As

Publication number Publication date
WO1998035058A2 (fr) 1998-08-13

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