WO1998033898A3 - Quantitative detection of phenylalanine and tyrosine in whole blood - Google Patents

Quantitative detection of phenylalanine and tyrosine in whole blood Download PDF

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Publication number
WO1998033898A3
WO1998033898A3 PCT/US1998/001822 US9801822W WO9833898A3 WO 1998033898 A3 WO1998033898 A3 WO 1998033898A3 US 9801822 W US9801822 W US 9801822W WO 9833898 A3 WO9833898 A3 WO 9833898A3
Authority
WO
WIPO (PCT)
Prior art keywords
phenylalanine
tyrosine
whole blood
quantitative detection
acids
Prior art date
Application number
PCT/US1998/001822
Other languages
French (fr)
Other versions
WO1998033898A2 (en
Inventor
Herbert Di Essien
Donald J Bartling
Steven R Binder
Sergey Vlasenko
Original Assignee
Bio Rad Laboratories
Essien Chris & Hm
Donald J Bartling
Steven R Binder
Sergey Vlasenko
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Rad Laboratories, Essien Chris & Hm, Donald J Bartling, Steven R Binder, Sergey Vlasenko filed Critical Bio Rad Laboratories
Priority to AU60511/98A priority Critical patent/AU6051198A/en
Priority to JP10533111A priority patent/JP2000515388A/en
Priority to EP98903853A priority patent/EP0970197A2/en
Publication of WO1998033898A2 publication Critical patent/WO1998033898A2/en
Publication of WO1998033898A3 publication Critical patent/WO1998033898A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/527—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Whole blood is analyzed for phenylalanine and tyrosine by first subjecting a specimen of the blood to the enzymatic action of phenylalanine ammonia lyase to selectively convert the two amino acids to trans-cinnamic and trans-coumaric acids, respectively, then analyzing the sample containing the converted acids by cation exchange chromatography.
PCT/US1998/001822 1997-01-31 1998-01-30 Quantitative detection of phenylalanine and tyrosine in whole blood WO1998033898A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU60511/98A AU6051198A (en) 1997-01-31 1998-01-30 Quantitative detection of phenylalanine and tyrosine in whole blood
JP10533111A JP2000515388A (en) 1997-01-31 1998-01-30 Quantitative detection of phenylalanine and tyrosine in whole blood
EP98903853A EP0970197A2 (en) 1997-01-31 1998-01-30 Quantitative detection of phenylalanine and tyrosine in whole blood

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US79249797A 1997-01-31 1997-01-31
US08/792,497 1997-01-31

Publications (2)

Publication Number Publication Date
WO1998033898A2 WO1998033898A2 (en) 1998-08-06
WO1998033898A3 true WO1998033898A3 (en) 1998-10-08

Family

ID=25157088

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/001822 WO1998033898A2 (en) 1997-01-31 1998-01-30 Quantitative detection of phenylalanine and tyrosine in whole blood

Country Status (4)

Country Link
EP (1) EP0970197A2 (en)
JP (1) JP2000515388A (en)
AU (1) AU6051198A (en)
WO (1) WO1998033898A2 (en)

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
F. W. SPIERTO, W. WHITFIELD, M. APETZ, W. H. HANNON: "Liquid-Chromatographic Measurement of Phenylalanine and Tyrosine in Serum", CLINICAL CHEMISTRY, vol. 28, no. 11, 1982, pages 2282 - 2285, XP002067024 *
R. S. HEDGE, M. J. MEHTA, R. A. BHATT, D. J. PATEL, S. L. BAFNA: "Molecular Sorption on Ion-Exchange Resins: Studies with Coumarin", JOURNAL OF PHARMACEUTICAL SCIENCES, vol. 57, no. 4, April 1968 (1968-04-01), pages 598 - 601, XP002067025 *
R-S. SHEN, C. W. ABELL: "Phenylketonuria: A New Method for the Simultaneous Determination of Plasma Phenylalanine and Tyrosine", SCIENCE, vol. 197, no. 4298, 1 July 1977 (1977-07-01), pages 665 - 667, XP002067026 *

Also Published As

Publication number Publication date
AU6051198A (en) 1998-08-25
EP0970197A2 (en) 2000-01-12
JP2000515388A (en) 2000-11-21
WO1998033898A2 (en) 1998-08-06

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