WO1998028971A3 - Transgenic animals for in vivo visualization of beta-amyloid toxicity - Google Patents

Transgenic animals for in vivo visualization of beta-amyloid toxicity Download PDF

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Publication number
WO1998028971A3
WO1998028971A3 PCT/US1997/023819 US9723819W WO9828971A3 WO 1998028971 A3 WO1998028971 A3 WO 1998028971A3 US 9723819 W US9723819 W US 9723819W WO 9828971 A3 WO9828971 A3 WO 9828971A3
Authority
WO
WIPO (PCT)
Prior art keywords
beta
transgenic animals
vivo visualization
amyloid toxicity
visualization
Prior art date
Application number
PCT/US1997/023819
Other languages
French (fr)
Other versions
WO1998028971A2 (en
Inventor
Christopher Link
Original Assignee
Univ Technology Corp
Christopher Link
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Technology Corp, Christopher Link filed Critical Univ Technology Corp
Priority to AU56192/98A priority Critical patent/AU5619298A/en
Priority to EP97952623A priority patent/EP0948612A2/en
Publication of WO1998028971A2 publication Critical patent/WO1998028971A2/en
Publication of WO1998028971A3 publication Critical patent/WO1998028971A3/en

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
    • A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
    • A01K67/0335—Genetically modified worms
    • A01K67/0336—Genetically modified Nematodes, e.g. Caenorhabditis elegans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4711—Alzheimer's disease; Amyloid plaque core protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/20—Animal model comprising regulated expression system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/70—Invertebrates
    • A01K2227/703—Worms, e.g. Caenorhabdities elegans
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0306—Animal model for genetic diseases
    • A01K2267/0312—Animal model for Alzheimer's disease
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0337—Animal models for infectious diseases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0393—Animal model comprising a reporter system for screening tests
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Wood Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Physics & Mathematics (AREA)
  • Neurology (AREA)
  • Veterinary Medicine (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to methods and compositions for visualization of the toxic effects of transgenes in vivo. In particular, the present invention provides methods and compositions for the production and use of transgenic, including dually transgenic, Caenorhabditis elegans for visualization of the toxic effects of β-amyloid accumulation in vivo.
PCT/US1997/023819 1997-01-03 1997-12-31 Transgenic animals for in vivo visualization of beta-amyloid toxicity WO1998028971A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU56192/98A AU5619298A (en) 1997-01-03 1997-12-31 Beta-amyloid toxicity
EP97952623A EP0948612A2 (en) 1997-01-03 1997-12-31 Beta-amyloid toxicity

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US77864597A 1997-01-03 1997-01-03
US8022297P 1997-01-03 1997-01-03
US08/778,645 1997-01-03
US60/080,222 1997-01-03

Publications (2)

Publication Number Publication Date
WO1998028971A2 WO1998028971A2 (en) 1998-07-09
WO1998028971A3 true WO1998028971A3 (en) 1998-10-29

Family

ID=26763238

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/023819 WO1998028971A2 (en) 1997-01-03 1997-12-31 Transgenic animals for in vivo visualization of beta-amyloid toxicity

Country Status (3)

Country Link
EP (1) EP0948612A2 (en)
AU (1) AU5619298A (en)
WO (1) WO1998028971A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1294450B1 (en) * 1997-08-28 1999-03-25 Consiglio Nazionale Ricerche TRANSGENIC ANIMALS FOR THE STUDY OF PHYSICAL OR BIOLOGICAL CHEMICAL TOXIC AGENTS
AU6291899A (en) * 1998-10-09 2000-05-01 Regents Of The University Of California, The Viable prp (prion protein) transgenic animals and methods of use
DE19849073A1 (en) * 1998-10-24 2000-04-27 Aventis Pharma Gmbh Transgene encoding amyloid precursor protein or its fragment, used to produce transgenic nematodes used, e.g. to screen for agents for treating Alzheimer's disease
EP1141359B1 (en) 1998-12-17 2006-10-04 Xenogen Corporation Non-invasive evaluation of physiological response in a mammal
GB9906018D0 (en) * 1999-03-16 1999-05-12 Devgen Nv Expression of DNA or proteins in C.elegans
AU2589500A (en) * 1999-09-03 2001-04-10 Xenogen Corporation Targeting constructs and transgenic animals produced therewith
US6867348B1 (en) 1999-12-16 2005-03-15 Xenogen Corporation Methods and compositions for screening for angiogenesis modulating compounds
IT1311335B1 (en) 1999-12-21 2002-03-12 Otc Optical Technologies Ct S PROCEDURE AND DEVICE FOR MAKING FIBER OPTIC LICENSES.
JP3354918B2 (en) 2000-06-08 2002-12-09 佑子 若松 Transparent medaka
US7741098B2 (en) 2001-11-27 2010-06-22 Nexyte Ab Production of eukaryotic proteins and nucleic acid molecules in C. elegans
FR2840320B1 (en) * 2002-05-30 2007-07-20 Centre Nat Rech Scient XENOPE TRANSGENIC EMBRYOS AND USES THEREOF FOR THE DETECTION OF ENDOCRINE DISRUPTORS AND METHODS THEREOF
GB0517977D0 (en) * 2005-09-02 2005-10-12 Birkeland Innovasjon As Transgenic animal model for identifying modulators of protein kinase
DE102007035238A1 (en) * 2007-07-27 2009-01-29 Christian-Albrechts-Universität Zu Kiel Biological effect monitoring by transgenic Hydra
WO2011017319A1 (en) 2009-08-03 2011-02-10 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Methods of treating disorders associated with protein polymerization
US9072772B2 (en) 2009-11-05 2015-07-07 University of Pittsburgh—of the Commonwealth System of Higher Education Methods of treating disorders associated with protein aggregation
US8809617B2 (en) * 2009-11-05 2014-08-19 The University of Pittsburgh—Of the Commonwealth System of Higher Education Automated high-content live animal drug screening using C. elegans
CN104920307B (en) * 2015-06-19 2018-01-26 安徽省农业科学院农业工程研究所 A kind of Caenorthaditis elegans ws123 fixing means suitable for single-particle microbeam device

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993023533A1 (en) * 1992-05-08 1993-11-25 Exemplar Corporation Anti-neoplastic in vivo drug screen
CA2088379A1 (en) * 1993-01-29 1994-07-30 University Of British Columbia Biological systems incorporating stress-inducible genes and reporter constructs for environmental biomonitoring and toxicology
WO1995011968A1 (en) * 1993-10-27 1995-05-04 Athena Neurosciences, Inc. Transgenic animals harboring app allele having swedish mutation

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993023533A1 (en) * 1992-05-08 1993-11-25 Exemplar Corporation Anti-neoplastic in vivo drug screen
CA2088379A1 (en) * 1993-01-29 1994-07-30 University Of British Columbia Biological systems incorporating stress-inducible genes and reporter constructs for environmental biomonitoring and toxicology
WO1995011968A1 (en) * 1993-10-27 1995-05-04 Athena Neurosciences, Inc. Transgenic animals harboring app allele having swedish mutation

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CHALFIE M ET AL: "GREEN FLUORESCENT PROTEIN AS A MARKER FOR GENE EXPRESSION", SCIENCE, vol. 263, no. 5148, 11 February 1994 (1994-02-11), pages 802 - 805, XP002003599 *
LINK, C.D.: "Expression of human beta-amyloid peptide in transgenic Caenorhabditis elegans", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA., vol. 92, no. 20, 26 September 1995 (1995-09-26), WASHINGTON US, pages 9368 - 9372, XP002065639 *
LINK, C.D.: "Transgenic Caenorhabditis elegans as model system to study amyloid formation and toxicity", NEUROBIOLOGY OF AGING, vol. 15, no. suppl. 1, 29 July 1994 (1994-07-29), pages S33, XP002065640 *
MOSS, J. B. ET AL.: "Green fluorescent protein marks skeletal muscle in murine cell lines and zebrafish", GENE, vol. 173, no. 1, 1996, AMSTERDAM NL, pages 89 - 98, XP002065641 *

Also Published As

Publication number Publication date
AU5619298A (en) 1998-07-31
WO1998028971A2 (en) 1998-07-09
EP0948612A2 (en) 1999-10-13

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