WO1998015293A3 - Apport cible de polymeres solubles a des proteines de recombinaison - Google Patents

Apport cible de polymeres solubles a des proteines de recombinaison Download PDF

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Publication number
WO1998015293A3
WO1998015293A3 PCT/US1997/018104 US9718104W WO9815293A3 WO 1998015293 A3 WO1998015293 A3 WO 1998015293A3 US 9718104 W US9718104 W US 9718104W WO 9815293 A3 WO9815293 A3 WO 9815293A3
Authority
WO
WIPO (PCT)
Prior art keywords
soluble polymers
recombinant proteins
targeted addition
protein
modification
Prior art date
Application number
PCT/US1997/018104
Other languages
English (en)
Other versions
WO1998015293A2 (fr
Inventor
Brian Seed
Wen Shao
John Seed
Original Assignee
Advanced Genetic Technology Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanced Genetic Technology Co filed Critical Advanced Genetic Technology Co
Priority to EP97945549A priority Critical patent/EP1011730A2/fr
Priority to AU46722/97A priority patent/AU4672297A/en
Publication of WO1998015293A2 publication Critical patent/WO1998015293A2/fr
Publication of WO1998015293A3 publication Critical patent/WO1998015293A3/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/61—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule the organic macromolecular compound being a polysaccharide or a derivative thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/59—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
    • A61K47/60—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol

Landscapes

  • Health & Medical Sciences (AREA)
  • Epidemiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

On modifie des protéines avec des polymères solubles afin de modifier leurs propriétés biologiques, de manière à les rendre moins immunogènes et à prolonger leur durée de vie dans l'organisme. On effectue cette modification au moyen d'une complexation forte mais non covalente entre un chélate de métal et un marqueur d'oligohistidine introduit dans la protéine par génie génétique. Ce procédé de modification de protéines permet d'obtenir des préparations homogènes qu'on peut préparer en quantités importantes.
PCT/US1997/018104 1996-10-07 1997-10-07 Apport cible de polymeres solubles a des proteines de recombinaison WO1998015293A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP97945549A EP1011730A2 (fr) 1996-10-07 1997-10-07 Apport cible de polymeres solubles a des proteines de recombinaison
AU46722/97A AU4672297A (en) 1996-10-07 1997-10-07 Targeted addition of soluble polymers to recombinant proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US2809196P 1996-10-07 1996-10-07
US60/028,091 1996-10-07

Publications (2)

Publication Number Publication Date
WO1998015293A2 WO1998015293A2 (fr) 1998-04-16
WO1998015293A3 true WO1998015293A3 (fr) 1998-06-18

Family

ID=21841531

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/018104 WO1998015293A2 (fr) 1996-10-07 1997-10-07 Apport cible de polymeres solubles a des proteines de recombinaison

Country Status (3)

Country Link
EP (1) EP1011730A2 (fr)
AU (1) AU4672297A (fr)
WO (1) WO1998015293A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6540897B1 (en) * 2000-07-13 2003-04-01 Pierce Chemical Company Direct detection of histidine tagged biomolecules on electrophoretic gel
US7045283B2 (en) 2000-10-18 2006-05-16 The Regents Of The University Of California Methods of high-throughput screening for internalizing antibodies
CN108586291A (zh) * 2018-01-02 2018-09-28 成都傲飞生物化学品有限责任公司 一种n，n-双（羧甲基）-l-赖氨酸的生产工艺

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993010233A1 (fr) * 1991-11-11 1993-05-27 Fidia S.P.A. Synthese et purification des formes tronquees et proteiniques mutantes du facteur neurotrophique ciliaire de l'homme
WO1995001797A1 (fr) * 1993-07-09 1995-01-19 Smithkline Beecham Corporation Purification de proteines
WO1996009805A2 (fr) * 1994-09-23 1996-04-04 Zonagen, Inc. Immunostimulation induite par le chitosane

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993010233A1 (fr) * 1991-11-11 1993-05-27 Fidia S.P.A. Synthese et purification des formes tronquees et proteiniques mutantes du facteur neurotrophique ciliaire de l'homme
WO1995001797A1 (fr) * 1993-07-09 1995-01-19 Smithkline Beecham Corporation Purification de proteines
WO1996009805A2 (fr) * 1994-09-23 1996-04-04 Zonagen, Inc. Immunostimulation induite par le chitosane

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GERSHON P.D. ET AL: "Stable chelating linkage for reversible immobilization of oligohistidine tagged proteins in the BIAcore surface plasmon resonance detector", JOURNAL OF IMMUNOLOGICAL METHODS, 1995, 183/1 SPEC. ISSUE (65-76), NETHERLANDS, XP004021026 *
HOCHULI E. ET AL.: "New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues", J. CHROMATOGRAPHY, vol. 411, 1987, pages 177 - 184, XP002059738 *
PORATH J. ET AL: "Metal chelate affinity chromatography, a new approach to protein fractionation", NATURE, vol. 258, 18 December 1975 (1975-12-18), pages 598 - 599, XP002059246 *

Also Published As

Publication number Publication date
EP1011730A2 (fr) 2000-06-28
AU4672297A (en) 1998-05-05
WO1998015293A2 (fr) 1998-04-16

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