METHOD FOR PREPARING SUBSTITUTED l-PIPERIDINECARBOXAMIDE DERIVATIVES
Summary of the Invention
This invention provides an improved process for producing the compounds of the formula (1.0):
wherein all substituents are as described below, which comprises reacting a compound of the formula (1.0)'
with an excess of urea in water.
The compounds of formula (1.0) are useful for inhibiting tumor growth. Examples of tumors which may be inhibited include, but are not limited to, lung cancer (e.g., lung adenocarcinoma), pancreatic cancers (e.g., pancreatic carcinoma such as, for example, exocrine pancreatic
carcinoma), colon cancers (e.g., colorectal carcinomas, such as, for example, colon adenocarcinoma and colon adenoma), myeloid leukemias (for example, acute myelogenous leukemia (AML)), thyroid follicular cancer, myelodysplastic syndrome (MDS), bladder carcinoma and epidermal carcinoma.
The compounds of formula (1.0) are disclosed in copending application, Ser. No. 08/410,187 filed March 24, 1995, which is hereby incorporated by reference.
The compounds of formula (1.0)' may be prepared by procedures disclosed in WO 95/10516 published April 20, 1995, which is hereby incorporated by reference.
Compounds of the invention may be prepared according to the procedures described in WO 95/10516 published April 20, 1995, copending Application Serial No. 08/410,187 filed March 24, 1995, copending Application Serial No. 08/577,951 filed December 22, 1995, and copending Application Serial No. 08/615,760 filed March 13, 1996; the disclosures of each being incoφorated herein by reference thereto; and according to the procedures described below.
The preparation of certain starting materials of the formula (1.0)' is described in the preparatory examples below. All starting materials of the formula (1.0)' can be prepared by the methods described in the preparatory examples below, or by methods analogous to those described in the preparatory examples below. Moreover, the preparation of final products of the formula (1.0) are also described in the preparatory examples below.
DETAILED DESCRIPTION OF THE INVENTION
This invention provides an improved process for producing the compounds of the formula (1.0):
which comprises reacting a compound of formula(I.O)'
with an excess of urea in water;
wherein:
X is N, CH, or C when the double bond is present at the C-11 position; one of a, b, c and d represents N or NR9 wherein R9 is O', -CH3 or -(CH2)nCθ2H wherein n is 1 to 3, and the remaining a, b, c and d groups represent CR1 or CR2; or each of a, b, c, and d are independently selected from CR1 or CR ;
each R1 and each R2 is independently selected from H, halo, -CF3, -OR10 (e.g., -OCH3), -COR™, -SR™ (e.g., -SCH3 and -SCH2C6H5), -S(0)(R11 (wherein t is 0, 1 or 2, e.g., -SOCH3 and -SO2CH3), -SCN, -N(R10)2, -NR1<>R11, -N02) -OC(0)R1°, -C02R1°, -OCO2R11, -CN, -NHC(0)R10, -NHSO2R10, -CONHR1°, -CONHCH2CH2OH, -NR^COOR11,
-SR 1C(0)OR1 (e.g., -SCH2CO2CH3), -SR11N(R12)2 wherein each R12 is independently selected from H and -C(0)OR11 (e.g., -S(CH2)2NHC(0)0-t-butyl and -S(CH )2NH2), benzotriazol-1-yloxy, tetrazol-5-ylthio, or substituted tetrazol-5-ylthio (e.g., alkyl substituted tetrazol-5-ylthio such as 1-methyl-tetrazol-5-ylthio), alkynyl, alkenyl or alkyl, said alkyl or alkenyl group optionally being substituted with halo, -ORi0 or -CO2R10; R3 and R4 are the same or different and each independently represents H. any of the substituents of R1 and R2, or R3 and R4 taken together represent a saturated or unsatu rated C5-C7 fused ring to the benzene ring (Ring III);
R5, R6, R7 and R8 each independently represents H, -CF3, -COR10, alkyl or aryl, said alkyl or aryl optionally being substituted with
-OR , -SR10, -S(0)tR11, -NR10COOR11, -N(R10)2, -N02, -COR10, -OCOR™, -OCO2R11, -CO2R10, OPO3R10, or R5 is combined with R6 to represent =0 or =S and or R7 is combined with R8 to represent =0 or =S; R10 represents H, alkyl, aryl, or aralkyl (e.g., benzyl);
R11 represents alkyl or aryl; the dotted line between carbon atoms 5 and 6 represents an optional double bond, such that when a double bond is present, A and B independently represent -R10, halo, -OR11, -OCO2R11 or -OC(0)R1°, and when no double bond is present between carbon atoms 5 and 6, A
and B each independently represent H2, -(0R11)2; H and halo, dihalo, alkyl and H, (alkyl)2, -H and -OC(0)R1°, H and -OR10, =0, aryl and H, =NOR10 or -0-(CH2)p-0- wherein p is 2, 3 or 4.
This process comprises reacting compounds of formula (1.0)' as described above with excess urea in water to obtain the compounds of formula (1.0)
A preferred process of the invention comprises using a compound of formula (1.0') wherein a is N; R5. Re, R7. and Re are all H; and R-|. R2, R3. and R4, are each independently selected from the group consisting of H or halo.
A more preferred process of the invention comprises using a compound of formula (1.0') as described just above wherein Ri _ is H; and R2 is Br; and R3, and R^ are each independently selected from the group consisting of Br and Cl.
A more preferred process of the invention comprises using a compound of formula (1.0') as described just above wherein X is CH.
A more preferred process of the invention comprises using a compound of formula (1.0') as described just above wherein R3 is Br; and R_t_ is Cl.
In a more specific embodiment, this invention provides a process for producing compounds of the formulae:
and m
which comprises reacting either II or IV
π or rv with an excess of urea in water. This reaction is run with about 4 to about 10 equivalents of urea as compared to the compounds of formula π, or IV more preferably about 10 equivalents of urea. The reaction is run for about 3 to about 68 hours, more preferably about 60 to 70 hours. The reaction is usually run at the reflux temperature of the reaction mixture. This can range from about 98 to about 100°C. The ratio of reactants II or IV to water may typically vary from about 0.025g/ml to about 0.6g/ml more preferably about 0.1 g/ml. The respective carboxamides of formulae I and III so obtained can be isolated by conventional means such as evaporation of the reaction mixture followed by chromatography on silica gel.
The process of the invention is typically run with the reactants II or IV which are insoluble in water, in a heterogeneous
mixture with the aqueous urea solution. Typically, the reactants II or IV may partially, or fully melt at some point during the reaction, but the reaction remains heterogeneous.
All of the compounds of formula (1.0) above can be prepared using the procedures described above, or can be prepared by procedures analogous to those described just above.
Literature procedures (F. Kurzer, Org. Synth., Collec, Vol. 4, 52 (1953)) require the conversion of water insoluble amines into their acid addition salts, which is usually done in SUM in order to obtain water soluble forms of the amine for reaction with aqueous solutions of urea at about 100°C. The present process of the invention is carried out without the formation of the acid addition salts of the amine.
As used herein, alkyl-(including the alkyl portions of alkoxy, alkylamino and dialkylamino)-represents straight and branched carbon chains and contains from one to twenty carbon atoms, preferably one to six carbon atoms; alkanediyl-represents a divalent, straight or branched hydrocarbon chain having from 1 to 20 carbon atoms, preferably 1 to 6 carbon atoms, the two available bonds being from the same or different carbon atoms thereof, e.g., methylene, ethylene, ethylidene, -CH2CH2CH2-, -CH2CHCH3, -CHCH2CH3, etc. cycloalkyl-represents saturated carbocyclic rings branched or unbranched of from 3 to 20 carbon atoms, preferably 3 to 7 carbon atoms; alkenyl-represents straight and branched carbon chains having at least one carbon to carbon double bond and containing from 2 to 12 carbon atoms, preferably from 2 to 6 carbon atoms and most preferably from 3 to 6 carbon atoms; alkynyl-represents straight and branched carbon chains having at least one carbon to carbon triple bond and containing from 2 to 12 carbon atoms, preferably from 2 to 6 carbon atoms;
aryl (including the aryl portion of aryloxy and aralkyl)-represents a carbocyclic group containing from 6 to 15 carbon atoms and having at least one aromatic ring (e.g., aryl is a phenyl ring), with all available substitutabie carbon atoms of the carbocyclic group being intended as possible points of attachment, said carbocyclic group being optionally substituted (e.g., 1 to 3) with one or more of halo, alkyl, hydroxy, alkoxy, phenoxy, CF3, amino, alkylamino, dialkylamino, -COOR10 or -Nθ2; and halo-represents fluoro, chloro, bromo and iodo; and Reference to the position of the substituents R1 , R2, R3, and R4 is based on the numbered ring structure:
For example, R1 can be at the C-4 position and R2 can be at the C-2 or C-3 position. Also, for example, R3 can be at the C-8 position and R4 can be at the C-9 position.
When the bond from the IV ring to the C-11 carbon is a single bond, all stereoisomers are included within formula (1.0), that is, racemates, R-isomers, and S-isomers.
Compounds of Formula 1.0 include compounds wherein the bottom piperidinyl group is a 4- or 3-piperidinyl group, i.e.,
Compounds of Formula 1.0 include compounds wherein R
2 and R
4 are H, and R
1 and R
3 are halo (preferably independently selected from Br or Cl). For example, R
1 is Br and R
3 is Cl. These compounds include compounds wherein R
1 is in the 3-position and R
3 is in the 8- position, e.g., 3-Br and 8-CI. Compounds of Formula 1.0 also include compounds wherein R
2 is H, and R
1, R
3 and R
4 are halo (preferably independently selected from Br or Cl).
Preferably, compounds of Formula 1.0 are represented by compounds of Formula 1.1 :
wherein all substituents are as defined for Formula 1.0.
Preferably, R2 is H and R1, R3 and R4 are halo; a is N and b, c and d are carbon; A and B are each H2; the optional bond between C5 and C6 is absent; X is CH; and R5, R6, R7 and R8 are H. More preferably, R1, R3 and R4 are independently selected from Br or Cl. Most preferably, R1 is Br, and R3 and R4 are independently selected from Cl and Br.
More preferably, compounds of Formula 1.0 are represented by compounds of Formula 1.2 and Formula 1.3:
a s of Formulas 1.4 and 1.5
wherein R
1 , R
3 and R
4 are each independently selected from halo, preferably, Br or Cl; and A, B, X and W are as defined for Formula 1.0. More preferably, A and B are each H2; the optional bond between C5 and C6 is absent; and X is CH. Most preferably, R
1 is Br; R
3 and R
4 are independently Br or Cl, and still more preferably R
3 is Cl and R
4 is Br; A and B are each H
2; the optional bond between C5 and C6 is absent; X is CH; and R*, R
6, R
7 and R
3 are H.
Examples of -C(0)R12 substituents for W include groups wherein R12 is sel
Examples of imidates for substituent W include groups wherein R13 is selected from the group consisting of: (1) CN; (2) H; (3) -Sθ2NR15R16 wherein R15 and R16 are selected from the group consisting of: H and alkyl (e.g., methyl); (4) -C(0)NR15R16 wherein R15 and R16 are selected from the group consisting of: H and alkyl (e.g., methyl); (5) -Sθ2-alkyl; and (6) -C(0)-aryl. Examples of imidates also include groups wherein R14 is aryl (e.g., phenyl).
For example, imidates for substituent W include groups wherein R13 is selected from the group consisting of: CN, -C(0)NH2, H, -SO2NH2, -SO2NHCH3, -Sθ2N(CH3)2, -C(0)NHCH3, -SO2CH3 and -C(0)C6H5* Exampes of imidates also include groups wherein R14 is phenyl; and R13 is selected from the group consisting of: CN, -C(0)NH2,
H, -S02NH2, -S02NHCH3, -S02N(CH3)2, -C(0)NHCH3, -S02CH3 and
-C(0)C6H5*
Examples of guanidines for substituent W include groups wherein
R17 is selected from the group consisting of: (1) CN; (2) H; (3) -OR22; (4) -NR20R21 wherein R20 and R21 are independently selected from the group consisting of: H and alkyl (e.g., methyl); (5) -SO2NR20R21 wherein
R20 and R21 are independently selected from the group consisting of: H and alkyl (e.g., methyl), (6) -C(O)NR 0R21 wherein R20 and R21 are independently selected from the group consisting of: H and alkyl (e.g., methyl); (7) -Sθ2-alkyl; and (8) -C(0)-aryl. Examples of the guanidines also include groups wherein R18 and R19 are selected from the group consisting of: H and heteroaralkyl.
For example, guanidines for substituent W include groups wherein R17 is selected from the group consisting of: CN, H, -OCH3 , -OH,-NH2, -N(CH3)2, -S0 NH2l -SO2NHCH3, -Sθ2N(CH3)2. -C(0)NH2,
-C(0)NHCH3, -SO2CH3 and -C(0)C6H5* Examples of guanidines also include groups wherein R18 and R19 are selected from the group consisting of: H and
Examples of the guanidine substituents additionally include groups wherein: R18 and R19 are selected from the group consisting of: H and -CH2-pyrid-3-yl); and R17 is selected from the group consisting of: CN, H, -OCH3 , -OH,-NH , -N(CH3)2, -S02NH2, -SO2NHCH3, -S02N(CH3)2. -C(0)NH2, -C(0)NHCH3, -SO2CH3 and -C(0)C6H5.
In addition, examples of the guanidine substituents additionally include groups wherein: (1) R17 and R18 are H, and R19 is -CH2-pyrid-3- yl; and (2) R18 and R19 are H, and R17 is selected from the group consisting of: CN, H, -OCH3 , -OH, -NH2, -N(CH3)2, -SO2NH2, -SO2NHCH3, -S02N(CH3)2, -C(0)NH2) -C(0)NHCH3, -S02CH3 and
-C(0)C6H5*
Examples of 1-amino-2-nitroethylene derivatives for substituent W include groups wherein R26 is alkyl, e.g., methyl.
Those skilled in the art will appreciate that compounds of Formula 1.0 include compounds of Formulas 1.0A and 1.OB:
wherein X is CH or N, with compounds of 1.0A being preferred when X is
CH, and with compounds of 1.0B being preferred when X is N.
The preferred compounds of this invention are represented by the compounds of Formulas:
wherein the substituents are as defined above, with the compounds of Formula 1.5A being more preferred.
The examples illustrate the process of this invention:
PREPARATORY EXAMPLES
PREPARATIVE EXAMPLE 1
Combine 10 g (60.5 mmol) of ethyl 4-pyridylacetate and 120 mL of dry CH2CI2 at -20°C, add 10.45 g (60.5 mmol) of MCPBA and stir at -20°C for 1 hour and then at 25°C for 67 hours. Add an additional 3.48 g (20.2 mmoles) of MCPBA and stir at 25°C for 24 hours. Dilute with CH2CI2 and wash with saturated NaHCθ3 (aqueous) and then water. Dry over MgSθ4, concentrate in vacuo to a residue, and chromatograph (silica gel, 2%-5.5% (10% NH4OH in MeOH)/CH2CI2)to give 8.12 g of the product compound. Mass Spec: MH+ = 182.15
Step B:
Combine 3.5 g (19.3 mmol) of the product of Step A, 17.5 mL of EtOH and 96.6 mL of 10% NaOH (aqueous) and heat the mixture at 67°C for 2 hours. Add 2 N HCI (aqueous) to adjust to pH = 2.37 and concentrate in vacuo to a residue. Add 200 mL of dry EtOH, filter throuπh celite® and wash the filter cake with dry EtOH (2X50 ml). Concentrate the combined filtrates in vacuo to give 2.43 g of the title compound.
PREPARATIVE EXAMPLE 2
The title compound is prepared via the process disclosed in PCT
International Publication No. WO95/10516.
PREPARATIVE EXAMPLE 3
Step A:
Combine 14.95 g (39 mmol) of 8-ch.oro-11-(1-ethoxy-carbonyl-4- piperidinyl)-11 H-benzo[5,6]cyclohepta[1,2-b]pyridine and 150 mL of CH2CI2, then add 13.07 g (42.9 mmol) of (nBu NNOs and cool the mixture to 0°C. Slowly add (dropwise) a solution of 6.09 mL (42.9 mmol) of TFAA in 20 mL of CH2CI2 over 1.5 hours. Keep the mixture at 0°C overnight, then wash successively with saturated NaHC03 (aqueous), water and brine. Dry the organic solution over Na2S04, concentrate in vacuo to a residue and chromatograph the residue (silica gel, EtOAc/hexane gradient) to give 4.32 g and 1.90 g of the two product compounds 3A(i) and 3A(ii), respectively.
Mass Spec, for compound 3A(i): MH+ = 428.2;
Mass Spec, for compound 3A(ii): MH+ = 428.3
Step β:
Combine 22.0 g (51.4 mmol) of the product 3A(i) from Step A, 150 mL of 85% EtOH (aqueous), 25.85 g (0.463 mole) of Fe powder and 2.42 g (21.8 mmol) of CaCtø, and heat at reflux overnight. Add 12.4 g (0.222 mole) of Fe powder and 1.2 g (10.8 mmol) of CaCtø and heat at reflux for 2 hours. Add another 12.4 g (0.222 mole) of Fe powder and 1.2 g (10.8 mmol) of CaCl2 and heat at reflux for 2 hours more. Filter the hot mixture through celite®, wash the celite® with 50 mL of hot EtOH and concentrate the filtrate in vacuo to a residue. Add 100 mL of anhydrous EtOH, concentrate to a residue and chromatograph the residue (silica gel, eOH/CH2Cl2 gradient) to give 16.47 g of the product compound.
Step C:
Combine 16.47 g (41.4 mmol) of the product from Step B, and 150 mL of 48% HBr (aqueous) and cool to -3°C. Slowly add (dropwise) 18 mL of bromine, then slowly add (dropwise) a solution of 8.55 g (0.124 mole) of NaNθ3 in 85 mL of water. Stir for 45 minutes at -3° to 0°C, then adjust to pH = 10 by adding 50% NaOH (aqueous). Extract with EtOAc, wash the extracts with brine and dry the extracts over Na2S04- Concentrate to a residue and chromatograph (silica gel, EtOAc/hexane gradient) to give 10.6 g and 3.28 g of the two product compounds 3C(i) and 3C(ii), respectively.
Mass Spec, for compound 3C(i): MH+ = 461.2;
Mass Spec, for compound 3C(ii): MH+ = 539
Hydrolyze the product 3C(i) of Step C by dissolving in concentrated HCI and heating to about 100°C for @ 16 hours. Cool the mixture, the neutralize with 1 M NaOH (aqueous). Extract with CH2CI2, dry the extracts over MgSθ4, filter and concentrate in vacuo to the title compound. Mass Spec: MH+ = 466.9
PREPARATIVE EXAMPLE 4
Combine 25.86 g (55.9 mmol) of 4-(8-chloro-3-bromo-5,6- dihydro-11H-benzo[5,6]cyclohepta[1 ,2-b]pyridin-11-ylidene)-1-
piperidine-1-carboxylic acid ethyl ester and 250 mL of concentrated H2S04 at -5°C, then add 4.8 g (56.4 mmol) of NaN03 and stir for 2 hours. Pour the mixture into 600 g of ice and basify with concentrated NH4OH (aqueous). Filter the mixture, wash with 300 mL of water, then extract with 500 mL of CH2CI2. Wash the extract with 200 mL of water, dry over MgS04, then filter and concentrate in vacuo to a residue. Chromatograph the residue (silica gel, 10% EtOAc/ CH2CI2) to give 24.4 g (86% yield) of the product, m.p. = 165-167°C, Mass Spec: MH+ = 506, 508 (Cl). elemental analysis: calculated - C, 52.13; H, 4.17; N, 8.29 found - C, 52.18; H. 4.51 ; N, 8.16
Step B:
Combine 20 g (40.5 mmol) of the product of Step A and 200 mL of concentrated H2SO4 at 20°C, then cool the mixture to 0°C. Add 7.12 g (24.89 mmol) of 1,3-dibromo-5,5-dimethyl-hydantoin to the mixture and stir for 3 hours at 20°C. Cool to 0°C, add an additional 1.0 g (3.5 mmol) of the dibromohydantoin and stir at 20°C for 2 hours. Pour the mixture into 400 g of ice, basify with concentrated NH4OH (aqueous) at 0°C, and collect the resulting solid by filtration. Wash the solid with 300 mL of water, slurry in 200 mL of acetone and filter to provide 19.79 g (85.6% yield) of the product, m.p. = 236-237°C, Mass Spec: MH+ = 586 (CI). elemental analysis: calculated - C, 45.11 ; H, 3.44; N, 7.17 found - C, 44.95; H, 3.57; N. 7.16
Step C:
Combine 25 g (447 mmol) of Fe filings, 10 g (90 mmol) of CaCl2 and a suspension of 20 g (34.19 mmol) of the product of Step B in 700 mL of 90:10 EtOH/water at 50°C. Heat the mixture at reflux overnight, filter through Celite® and wash the filter cake with 2 X 200 mL of hot EtOH. Combine the filtrate and washes, and concentrate in vacuo to a residue. Extract the residue with 600 mL of CH2CI2, wash with 300 mL of water and dry over MgS04. Filter and concentrate in vacuo to a residue, then chromatograph (silica gel, 30% E.OAC/CH2CI2) to give 11.4 g (60% yield) of the product, m.p. = 211-212°C, Mass Spec: MH+ = 556 (Cl). elemental analysis: calculated - C, 47.55; H, 3.99; N, 7.56 found - C, 47.45; H. 4.31 ; N, 7.49
Step D:
Slowly add (in portions) 20 g (35.9 mmol) of the product of Step C to a solution of 8 g (116 mmol) of NaNθ2 in 120 mL of concentrated HCI
(aqueous) at -10°C. Stir the resulting mixture at 0°C for 2 hours, then slowly add (dropwise) 150 mL (1.44 mole) of 50% H3PO2 at 0°C over a 1 hour period. Stir at 0°C for 3 hours, then pour into 600 g of ice and basify with concentrated NH4OH (aqueous). Extract with 2 X 300 mL of CH2CI2, dry the extracts over MgSθ4, then filter and concentrate in vacuo to a residue. Chromatograph the residue (silica gel, 25% EtOAc/ hexanes) to give 13.67 g (70% yield) of the product, m.p. = 163-165°C, Mass Spec: MH+ = 541 (Cl). elemental analysis: calculated - C, 48.97; H, 4.05; N, 5.22 found - C, 48.86; H, 3.91 ; N, 5.18 Step E:
Combine 6.8 g (12.59 mmol) of the product of Step D and 100 mL of concentrated HCI (aqueous) and stir at 85°C overnight. Cool the mixture, pour it into 300 g of ice and basify with concentrated NH4OH (aqueous). Extract with 2 x 300 mL of CH2CI2, then dry the extracts over MgSθ4. Filter, concentrate in vacuo to a residue, then chromatograph (silica gel, 10% MeOH/EtOAc + 2% NH4OH (aqueous)) to give 5.4 g (92% yield) of the title compound, m.p. = 172-174°C, Mass Spec: MH+ = 469 (FAB). elemental analysis: calculated - C, 48.69; H, 3.65; N, 5.97 found - C, 48.83; H, 3.80; N, 5.97
PREPARATIVE EXAMPLE 5
Hydrolyze 2.42 g of 4-(8-chloro-3-bromo-5,6-dihydro-11H- benzo[5,6]cyclohepta[1 ,2-b]pyridin-11 -ylidene)-1 -piperidine-1 -carboxylic acid ethyl ester via substantially the same procedure as described in Preparative Example 3, Step D, to give 1.39 g (69% yield) of the product. Step B:
Combine 1 g (2.48 mmol) of the product of Step A and 25 mL of dry toluene, add 2.5 mL of 1 M DIBAL in toluene and heat the mixture at reflux. After 0.5 hours, add another 2.5 mL of 1 M DIBAL in toluene and heat at reflux for 1 hour. (The reaction is monitored by TLC using 50%
MeOH CH2Cl2 +NH4OH (aqueous).) Cool the mixture to room temperature, add 50 mL of 1 N HCI (aqueous) and stir for 5 min. Add 100 mL of 1 N NaOH (aqueous), then extract with EtOAc (3 X 150 mL). Dry the extracts over MgSθ , filter and concentrate in vacuo Xo give 1.1 g of the title compound.
PREPARATIVE EXAMPLE 6
[racemic as well as (+)- and (-)-isomers] Step A:
Combine 16.6 g (0.03 mole) of the product of Preparative Example 4, Step D, with a 3:1 solution of CH3CN and water (212.65 mL CH3CN and 70.8 L of water) and stir the resulting slurry overnight at room temperature. Add 32.833 g (0.153 mole) of Nalθ4 and then 0.31 g (2.30 mmol) of Ruθ2 and stir at room temperature give 1.39 g (69% yield) of the product. (The addition of RuO is accompanied by an exothermic reaction and the temperature climbs from 20° to 30°C.) Stir the mixture for 1.3 hrs. (temperature returned to 25°C after about 30 min.), then filter to remove the solids and wash the solids with CH2CI2. Concentrate the filtrate in vacuo to a residue and dissolve the residue in
CH2CI2. Filter to remove insoluble solids and wash the solids with CH2CI2. Wash the filtrate with water, concentrate to a volume of about 200 mL and wash with bleach, then with water. Extract with 6 N HCI (aqueous). Cool the aqueous extract to 0°C and slowly add 50% NaOH (aqueous) to adjust to pH = 4 while keeping the temperature <30°C. Extract twice with CH2CI2, dry over MgSO and concentrate in vacuo to a residue. Slurry the residue in 20 mL of EtOH and cool to 0°C. Collect the resulting solids by filtration and dry the solids in vacuo to give 7.95 g of the product. 1 H NMR (CDCI3, 200 MHz): 8.7 (s, 1H); 7.85 (m, 6H); 7.5 (d, 2H); 3.45 (m, 2H); 3.15 (m, 2H). Step B:
Combine 21.58 g (53.75 mmol) of the product of Step A and 500 mL of an anhydrous 1 :1 mixture of EtOH and toluene, add 1.43 g (37.8 mmol) of NaBH4 and heat the mixture at reflux for 10 min. Cool the mixture to 0°C, add 100 mL of water, then adjust to pH= 4-5 with 1 M HCI (aqueous) while keeping the temperature <10°C. Add 250 mL of EtOAc and separate the layers. Wash the organic layer with brine (3 X 50 mL) then dry over Na2Sθ4* Concentrate in vacuo to a residue (24.01 g) and chromatograph the residue (silica gel, 30 % hexane/CH2Cl2) to give the product. Impure fractions were purified by rechromatography. A total of 18.57 g of the product was obtained. 1H NMR (DMSO-d6, 400 MHz): 8.5 (s, 1H); 7.9 (s, 1 H); 7.5 (d of d, 2H); 6.2 (s, 1H); 6.1 (s, 1H); 3.5 (m, 1H); 3.4 (m. 1 H); 3.2 (m, 2H).
Step C:
Combine 18.57 g (46.02 mmol) of the product of Step B and 500 mL of CHCI3, then add 6.70 mL (91.2 mmol) of SOCI2, and stir the mixture at room temperature for 4 hrs. Add a solution of 35.6 g (0.413 mole) of piperazine in 800 mL of THF over a period of 5 min. and stir the mixture for 1 hr. at rrom temperature. Heat the mixture at reflux overnight, then cool to room temperature and dilute the mixture with 1 L of CH2CI2- Wash with water (5 X 200 mL), and extract the aqueous wash with CHCI (3 X 100 mL). Combine all of the organic solutions, wash with brine (3 X 200 mL) and dry over MgS04. Concentrate in vacuo to a residue and chromatograph (silica gel, gradient of 5%, 7.5%, 10% MeOH CH2Cl2 + NH4OH) to give 18.49 g of the title compound as a racemic mixture.
Step D - Separation of Enantiomers:
The racemic title compound of Step C is separated by preparative chiral chromatography (Chiralpack AD, 5 cm X 50 cm column, flow rate 100 mL/min., 20% iPrOH/hexane + 0.2% diethylamine), to give 9.14 g of the (+)-isomer and 9.30 g of the (-)-isomer.
Physical chemical data for (+)-isomer: m.p. = 74.5°-77.5°C; Mass Spec. MH+ = 471.9; [a}^ = +97.4° (8.48 mg/ 2mL MeOH).
Physical chemical data for (-)-isomer: m.p. = 82.9°-84.5°C; Mass Spec. MH+ = 471.8; [a]£5 = -97.4° (8.32 mg/ 2mL MeOH).
PREPARATIVE EXAMPLE 7
Combine 15 g (38.5 mmol) of 4-(8-chloro-3-bromo-5,6-dihydro- 11 H-benzo[5,6]cyclohepta[1 ,2-b]pyridin-11-ylidene)-1-piperidine-1- carboxylic acid ethyl ester and 150 mL of concentrated H2SO4 at -5°C, then add 3.89 g (38.5 mmol) of KNO3 and stir for 4 hours. Pour the mixture into 3 L of ice and basify with 50% NaOH (aqueous). Extract with CH2CI2, dry over MgS04, then filter and concentrate in vacuo to a residue. Recrystallize the residue from acetone to give 6.69 g of the product. 1H NMR (CDCI3, 200 MHz): 8.5 (s, 1H); 7.75 (s, 1H); 7.6 (s, 1H); 7.35 (s, 1H); 4.15 (q, 2H); 3.8 (m, 2H); 3.5-3.1 (m, 4H); 3.0-2.8 (m, 2H); 2.6-2.2 (m, 4H); 1.25 (t, 3H).
Combine 6.69 g (13.1 mmol) of the product of Step A and 100 mL of 85% EtOH/water, then add 0.66 g (5.9 mmol) of CaC.2 and 6.56 g (117.9 mmol) of Fe and heat the mixture at reflux overnight. Filter the hot reaction mixture through celite® and rinse the filter cake with hot EtOH. Concentrate the filtrate in vacuo to give 7.72 g of the product. Mass Spec: MH+ = 478.0
Step C:
Combine 7.70 g of the product of Step B and 35 mL of HOAc, then add 45 mL of a solution of Br2 in HOAc and stir the mixture at room temperature overnight. Add 300 mL of 1 N NaOH (aqueous) , then 75 mL of 50% NaOH (aqueous) and extract with EtOAc. Dry the extract over MgSO and concentrate in vacuo to a residue. Chromatograph the residue (silica gel, 20%-30% EtOAc/hexane) to give 3.47 g of the product (along with another 1.28 g of partially purified product). Mass Spec: MH+ = 555.9.
1H NMR (CDCI3, 300 MHz): 8.5 (s, 1H); 7.5 (s, 1H); 7.15 (s, 1H); 4.5 (s, 2H); 4.15 (m, 3H); 3.8 (br s, 2H); 3.4-3.1 (m, 4H); 9-2.75 (m, 1H); 2.7- 2.5 (m, 2H); 2.4-2.2 (m, 2H); 1.25 (m, 3H).
Step D:
Combine 0.557 g (5.4 mmol) of t-butylnitrite and 3 mL of DMF, and heat the mixtre at to 60°-70°C. Slowly add (dropwise) a mixture of 2.00 g (3.6 mmol) of the product of Step C and 4 mL of DMF, then cool the mixture to room temperature. Add another 0.64 mL of t-butylnitrite at
40°C and reheat the mixture to 60°-70°C for 0.5 hrs. Cool to room temperature and pour the mixture into 150 mL of water. Extract with CH2CI2, dry the extract over gS0 and concentrate in vacuo to a residue. Chromatograph the residue (silica gel, 10%-20% EtOAc/hexane) to give 0.74 g of the product. Mass Spec: MH+ = 541.0. H NMR (CDCI3, 200 MHz): 8.52 (s, 1H); 7.5 (d, 2H); 7.2 (s, 1H); 4.15 (q, 2H); 3.9-3.7 (m, 2H); 3.5-3.1 (m, 4H); 3.0-2.5 (m, 2H); 2.4-2.2 (m, 2H); 2.1-1.9 (m, 2H); 1.26 (t, 3H).
Combine 0.70 g (1.4 mmol) of the product of Step D and 8 mL of concentrated HCI (aqueous) and heat the mixture at reflux overnight. Add 30 mL of 1 N NaOH (aqueous), then 5 mL of 50% NaOH (aqueous) and extract with CH2CI2. Dry the extract over MgSθ4 and concentrate in vacuo to give 0.59 g of the title compound. Mass Spec: M+ = 468.7. m.p. = 123.9°-124.2°C.
PREPARATIVE EXAMPLE 8
[racemic as well as (+)- and (-)-isomers]
Step A:
Prepare a solution of 8.1 g of the title compound from Freparative Example 7 in toluene and add 17.3 mL of a 1 M solution of DIBAL in toluene. Heat the mixture at reflux and slowly add (dropwise) another 21 mL of 1 M DIBALΛoluene solution over a period of 40 min. Cool the reaction mixture to about 0°C and add 700 mL of 1 M HCI (aqueous). Separate and discard the organic phase. Wash the aqueous phase with CH2CI2, discard the extract, then basify the aqueous phase by adding 50% NaOH (aqueous). Extract with CH2CI2, dry the extract over MgS04 and concentrate in vacuo to give 7.30 g of the title compound, which is a racemic mixture of enantiomers.
Step B - Separation of Enantiomers:
The racemic title compound of Step A is separated by preparative chiral chromatography (Chiralpack AD, 5 cm X 50 cm column, using 20% iPrOH/hexane + 0.2% diethylamine), to give the (+)-isomer and the (-)-isomer of the title compound.
Physical chemical data for (+)-isomer: m.p. = 148.8°C; Mass Spec MH+ = 472; [a]^ = +65.6° ( mg/ 2mL MeOH).
Physical chemical data for (-)-isomer: m.p. = 112°C; Mass Spec. MH+ = 472; [a]^ = -65.2° ( mg/ 2mL MeOH).
PREPARATIVE EXAMPLE 9
[racemic as well as (+)- and (-)-isomers]
Step A:
Combine 40.0 g (0.124 mole) of the starting ketone and 200 mL of H2SO4 and cool to 0°C. Slowly add 13.78 g (0.136 mole) of KNO3 over a period of 1.5 hrs., then warm to room temperature and stir overnight. Work up the reaction using substantially the same procedure as described for Preparative Example 4, Step A. Chromatograph (silica
gel, 20%, 30%, 40%, 50% EtOAc/hexane, then 100% EtOAc) to give 28 g of the 9-nitro product, along with a smaller quantity of the 7-nr.ro product and 19 g of a mixture of the 7-nitro and 9-nitro compounds.
Step B:
React 28 g (76.2 mmol) of the 9-nitro product of Step A, 400 mL of 85% EtOH/water, 3.8 g (34.3 mmol) of CaCtø and 38.28 g (0.685 mole) of Fe using substantially the same procedure as described for Preparative Example 4, Step C, to give 24 g of the product
Step C:
Combine 13 g (38.5 mmol) of the product of Step B, 140 mL of
HOAc and slowly add a solution of 2.95 mL (57.8 mmol) of Br2 in 10 mL of HOAc over a period of 20 min. Stir the reaction mixture at room temperature, then concentrate in vacuo X a residue. Add CH2CI2 and water, then adjust to pH = 8-9 with 50% NaOH (aqueous). Wash the organic phase with water, then brine and dry over Na2S04. Concentrate in vacuo to give 11.3 g of the product.
Step D:
Cool 100 L of concentrated HCI (aqueous) to 0°C, then add 5.61 g (81.4 mmol) of NaNθ2 and stir for 10 min. Slowly add (in portions) 11.3 g (27.1 mmol) of the product of Step C and stir the mixture at 0°-3°C for 2.25 hrs. Slowly add (dropwise) 180 mL of 50% H3PO2 (aqueous) and allow the mixture to stand at 0°C ovemight. Slowly add (dropwise) 150 mL of 50% NaOH over 30 min., to adjust to pH = 9, then extract with CH2CI
2* Wash the extract with water, then brine and dry over Na2S0
4. Concentrate in vacuo to a residue and chromatograph (silica gel, 2% EtOAc/ CH
2CI2) to give 8.6 g of the product.
Step E:
Combine 8.6 g (21.4 mmol) of the product of Step D and 300 mL of MeOH and cool to 0°-2°C. Add 1.21 g (32.1 mmol) of NaBH and stir the mixture at ~0°C for 1 hr. Add another 0.121 g (3.21 mmol) of NaBH4, stir for 2 hr. at 0°C, then let stand ovemight at 0°C. Concentrate in vacuo to a residue then partition the residue between CH2CI2 and water. Separate the organic phase and concentrate in vacuo (50°C) to give 8.2 g of the product.
Step F:
Combine 8.2 g (20.3 mmol) of the product of Step E and 160 L of CH2CI2, cool to 0°C, then slowly add (dropwise) 14.8 mL (203 mmol) of SOCI2 over a 30 min. period. Warm the mixture to room temperature
and stir for 4.5 hrs., then concentrate in vacuo to a residue, add CH2CI2 and wash with 1 N NaOH (aqueous) then brine and dry over Na2Sθ4. Concentrate in vacuo to a residue, then add dry THF and 8.7 g (101 mmol) of piperazine and stir at room temperature ovemight. Concentrate in vacuo to a residue, add CH2CI2, and wash with 0.25 N NaOH (aqueous), water, then brine. Dry over Na2S04 and concentrate in vacuo to give 9.46 g of the crude product. Chromatograph (silica gel, 5% MeOH/CH2Cl2 + NH3) to give 3.59 g of the title compound, as a racemate. 1H NMR (CDCI3. 200 MHz): 8.43 (d, 1 H); 7.55 (d, 1H); 7.45 (d, 1H); 7.11 (d, 1 H); 5.31 (s, 1 H); 4.86-4.65 (m, 1 H); 3.57-3.40 (m, 1 H); 2.98-2.55 (m, 6H); 2.45-2.20 (m, 5H).
Step G - Separation of Enantiomers:
The racemic title compound from Step F (5.7 g) is chromatographed as described for Preparative Example 6, Step D, using 30% iPrOH/hexane + 0.2% diethylamine, to give 2.88 g of the R- (+)-isomer and 2.77 g of the S-(-)-isomer of the title compound.
Physical chemical data for the R-(+)-isomer: Mass Spec. MH+ = 472.0; [a]^5 = +12.1 ° (10.9 mg/ 2mL MeOH).
Physical chemical data for the S-(-)-isomer: Mass Spec. MH+ =
,25
472.0; [a£ = -13.2° (11.51 mg/ 2mL MeOH).
PREPARATIVE EXAMPLE 10
[racemic as well as (+)- and (-)-isomers]
Step A:
Combine 13 g (33.3 mmol) of the title compound from Preparative Example 4, Step D, and 300 mL of toluene at 20°C, then add 32.5 L (32.5 mmol) of a 1 M solution of DIBAL in toluene. Heat the mixture at reflux for 1 hr., cool to 20°C, add another 32.5 mL of 1 M DIBAL solution and heat at reflux for 1 hr. Cool the mixture to 20°C and pour it into a mixture of 400 g of ice, 500 mL of EtOAc and 300 mL of 10% NaOH (aqueous). Extract the aqueous layer with CH2CI2 (3 x 200 mL), dry the organic layers over MgS04, then concentrate in vacuo to a residue. Chromatograph (silica gel, 12% MeOH/CH2Cl2 + 4% NH4OH) to give 10.4 g of the title compound as a racemate. Mass Spec: MH+ = 469 / 471 (FAB), partial 1H NMR (CDCI3, 400 MHz): 8.38 (s, 1H); 7.57 (s, 1 H); 7.27 (d, 1 H); 7.06 (d, 1 H); 3.95 (d, 1 H).
Step B - Separation of Enantiomers:
The racemic title compound of Step A is separated by preparative chiral chromatography (Chiralpack AD, 5 cm X 50 cm column, using 5% iPrOH/hexane + 0.2% diethylamine), to give the (+)-isomer and the (-)- isomer of the title compound.
Physical chemical data for (+)-isomer: Mass Spec. MH+ = 470.9, 472.8 (FAB); [a]p5 = +43.5° (c=0.402, EtOH); partial 1H NMR (CDCI3, 400 MHz): 8.38 (s, 1 H); 7.57 (s, 1 H); 7.27 (d, 1H); 7.05 (d, 1H); 3.95 (d, 1H).
Physical chemical data for (-)-isomer: Mass Spec. MH+ = 470.9, 472.8 (FAB); [a]^ = -41.8° (c=0.328 EtOH); partial 1H NMR (CDCI3, 400 MHz): 8.38 (s, 1H); 7.57 (s, 1H); 7.27 (d, 1H); 7.05 (d, 1H); 3.95 (d, 1 H).
PREPARATIVE EXAMPLE 11
[racemic as well as R-(+)- and S-(-)-isomers]
Treat 4-(8-chloro-3-bromo-5,6-dihydro-11H- benzo[5,6]cyclohepta[1 ,2-b]pyridin-11-ylidene)-1-piperidine-1 -carboxylic acid ethyl ester via substantially the same procedure as described in Preparative Example 6, Steps A-D, to give as the product of Step C, the racemic title compound, and as the products of Step D the R-(+)-isomer and S-(-)-isomer of the title compound.
Physical chemical data for the R-(+)-isomer: 13C NMR (CDCI3): 155.8 (C); 146.4 (CH); 140.5 (CH); 140.2 (C); 136.2 (C); 135.3 (C); 133.4 (C); 132.0 (CH); 129.9 (CH); 125.6 (CH); 119.3 (C); 79.1 (CH); 52.3 (CH2); 52.3 (CH); 45.6 (CH2); 45.6 (CH2); 30.0 (CH2); 29.8 (CH2). [ajo5 = +25.8° (8.46 mg/2 mL MeOH).
Physical chemical data for the S-(-)-isomer: 13C NMR (CDCI3): 155.9 (C); 146.4 (CH); 140.5 (CH); 140.2 (C); 136.2 (C); 135.3 (C); 133.3 (C); 132.0 (CH); 129.9 (CH); 125.5 (CH); 119.2 (C); 79.1 (CH); 52.5 (CH2); 52.5 (CH); 45.7 (CH2); 45.7 (CH2); 30.0 (CH2); 29.8 (CH2). [a]o5 = -27.9° (8.90 mg/2 mL MeOH).
EXAMPLE 1
Dissolve 1.160 g (2.98 mmol) of the title compound from Preparative Example 3 in 20 mL of DMF, stir at room temperature, and add 0.3914 g (3.87 mmol) of 4-methyl-morpholine, 0.7418 g (3.87 mmol) of DEC, 0.5229 g (3.87 mmol) of HOBT, and 0.8795 g (3.87 mmol) of 1- N-t-butoxycarbonyl-piperidinyl-4-acetic acid. Stir the mixture at room temperature for 2 days, then concentrate in vacuo to s residue and partition the residue between CH2CI2 and water. Wash the organic phase successively with saturated NaHCθ3 (aqueous), 10% NaH2P04 (aqueous) and brine. Dry the organic phase over MgSθ4, filter and concentrate in vacuo to a residue. Chromatograph the residue (silica gel, 2% MeOH/ CH2CI2 + NH3) to give 1.72 g of the product, m.p. = 94.0-94.5°C, Mass Spec: MH+ = 616.3 elemental analysis: calculated - C, 60.54; H, 6.06; N, 6.83 found - C, 59.93; H, 6.62; N, 7.45
Step B:
Combine 1.67 g (2.7 mmol) of the product of Step A and 20 mL of CH2CI2 and stir at 0°C. Add 20 mL of TFA, stir the mixture for 2 hours, then basify the mixture with 1 N NaOH (aqueous). Extract with CH2CI2. dry the organic phase over MgSθ4, filter and concentrate in vacuo to give 1.16 g of the product, m.p. = 140.2-140.8°C, Mass Spec: MH+ = 516.2.
Combine 0.50 g of the product of Step B, 20 mL of CH2CI2 and 4.5 equivalents of (CHsføSiNCO and stir at room temperature for 3 hours. Extract the mixture with saturated aHC03 (aqueous) and dry the organic phase over MgSθ4. Filter and concentrate in vacuo to give 0.8 g of the crude product. Chromatograph the crude product (silica gel, 5% MeOH/CH2Cl2 + NH3) to give 0.26 g of the product, m.p. = 170.2- 170.5°C, Mass Spec: MH+ s 559.1
EXAMPLE g
Combine 0.5 g (1.06 mmol) of the title compound of Preparative Example 4, 0.4 g (2.61 mmol) of the title compound of Preparative Example 1 , 5 mL of dry DMF, and 0.5 mL (4.53 mmol) of 4- methylmorpholine, at 0°C, then add 0.6 g (3.12 mmol) of DEC and 0.4 g (2.96 mmol) of HOBT an stir the mixture ovemight at 20°C. Concentrate in vacuo to a residue and extract the residue with CH2CI2 (2 X 50 mL). Wash the extracts with 25 mL of water, dry over MgSθ4, then concentrate in vacuo to a residue and chromatograph (silica gel, 10% MeOH/EtOAc + 2% NH4OH (aqueous)) to give 0.6 g (93.7% yield) of the title compound. Mass Spec: MH+ = 604.6 (FAB); partial 1H NMR (CDCI3, 300 MHz): 8.48 (s, 1H); 8.16 (d, 2H); 7.61 (s, 1H); 7.29 (m, 1H); 7.18 (d, 2H); 7.04 (d, 1H); 3.71 (s, 2H). elemental analysis: calculated - C, 48.81 ; H, 4.10; N, 6.57 found - C, 49.10; H, 3.79; N, 6.74 EXAMPLE 3
Dissolve 5.9 g (9.78 mmol) of the title compound of Example 2 in 300 mL of 1 :5 CH
2CI
2/EtOAc at 0°C. Slowly add (dropwise) 3 mL of 4 N HCI (aqueous) and stir the mixture at 0°C for 5 min. Add 200 mL of Et2θ, collect the resulting solids by filtration and wash the solids with 50 mL of E.2O. Dry the solids at 20°C and 0.2 mm Hg to give 5.9 g (96% yield) of the title compound. Mass Spec: MH+ = 604 (FAB), partial H NMR (DMSO-d
6, 300 MHz): d 8.66 (d, 2H); 8.51 (s, 1H); 7.95 (s, 1H); 7.67 (d, 2H); 7.47 (m, 1H); 7.15 (m, 1H); 3.99 (s, 2H). elemental analysis: calculated - C, 48.77; H, 3.62; N, 6.56 found - C, 48.34; H, 3.95; N, 6.84 EXAMPLE 4
Step A:
Combine 0.501 g (1.28 mmol) of the title compound of Preparative Example 5 and 20 mL of dry DMF, then add 0.405 g (1.664 mmol) of 1- N-t-butoxycarbonylpiperidinyl-4-acetic acid, 0.319 g (1.664 mmol) of DEC, 0.225 g (1.664 mmol) of HOBT, and 0.168 g (1.664 mmol) of 4- methylmorpholine and stir the mixture at room temperature overnight. Concentrate the mixture in vacuo to a residue, then partition the residue between 150 mL of CH2CI2 and 150 mL of saturated NaHCθ3
(aqueous). Extract the aqueous phase with another 150 mL of CH2CI2. Dry the organic phase over MgSθ4, and concentrate in vacuo to a residue. Chromatograph the residue (silica gel, 500 mL hexane, 1 L of 1% MeOH/CH2CI + 0.1% NH4OH (aqueous), then 1 L of 2% MeOH/CH2CI2 + 0.1% NH4OH (aqueous)) to give 0.575 g of the product, m.p. = 115°-125°C; Mass Spec: MH+ = 616. Step B:
Combine 0.555 g (0.9 mmol) of the product of Step A and 15 mL of CH2CI2 and cool the mixture to 0°C. Add 15 mL of TFA and stir at 0°C for 2 hours. Concentrate in vacuo at 40-45°C to a residue, then partition the residue between 150 mL of CH2CI2 and 100 mL of saturated NaHC03 (aqueous). Extract the aqueous layer with 100 mL of CH2CI2, combine the extracts and dry over gS04. Concentrate in vacuo to give 0.47 g of the product. m.p. = 140°-150°C; Mass Spec: MH+ = 516. Step C:
Combine 0.449 g (0.87 mmol) of the product of Step B, 20 mL of CH2CI2 and 0.501 g (0.59 mmol) of (CH3)3SiNCO and stir at room temperature ovemight. Add 50-75 mL of saturated NaHC03 (aqueous)
and stir for 0.5 hours. Dilute with CH2CI2. separate the layers and extract the aqueous layer with 2 X 100 mL of CH2CI2. Dry the combined CH2CI2 extracts over gSθ4 and concentrate in vacuo to a residue. Chromatograph the residue (silica gel, 500 mL CH2CI2; 1 L of 1% MeOH/CH2CI2 + 0.1% NH4OH; 1 L of 2% MeOH/CH2CI2 + 0.2% NH4OH; then with 3% MeOH/CH2CI + 0.3% NH4OH) to give 0.33 g of the title compound. m.p. = 145°-155°C; Mass Spec: MH+ = 559.
EXAMPLE 5
(-)-isomer
Combine 3.0 g (6.36 mmol) of the (-)-isomer the title compound from Preparative Example 6, Step D, and 70 mL of dry DMF. Add 3.84 mL (34.94 mmol) of N-methylmorpholine, 3.28 g (17.11 mmol) of DEC, 2.23 g (16.52 mmol) of HOBT and 2.09 (13.55 mmol) of 4-pyridylacetic acid N-oxide from Preparative Example 1 and stir the mixture at room temperature overnight. Concentrate in vacuo to remove the DMF, add 100 mL of saturated NaHCθ3 (aqueous) and 10 mL of CH2CI2 and stir for 15 min. Extract the mixture with CH2CI2 (2 X 500 mL), dry the extracts over MgS04 and concentrate in vacuo to a residue. Chromatograph the residue (500 g reverse phase C18 silica, gradient of
75%, 80%, then 85% MeOH/water + 0.1% HOAc). Concentrate the desired fractions in vacuo to remove MeOH and add 50 mL of 1 M NaOH (aqueous). Stir for 15 min., then extract with CH2CI2 (2 X 500 mL). Dry the extract over MgSθ4 and concentrate in vacuo to give 3.4 g of the title compound, m.p. = 148.9°-150.5°C; [a]" = -56.37° (9.4 mg/2mL MeOH); Mass Spec. MH
+ = 607.
The title compound of Example 5 can also be isolated as its HCI salt by treating a solution of the product in HCI and CH2CI2 at room temperature, followed by concentration in vacuo to give the HCI salt. [ajξ = -31.9° (4.80 mg 2 mL MeOH + 1 L of water). Using the (+)-isomer of the product of Preparative Example 6 and following essentially the same procedure as described above for Example 5, the analogous (+)-isomer (Example 5A), i.e., the enantiomer of the title compound of Example 5, is prepared, m.p. = 149.0°-150.5°C; Mass
The title compound of Example 5A can also be isolated as its HCI salt as described above for Example 5. m.p. = 152.9° (dec); [a]f = +41.7° (2 mL MeOH + 1 mL of water).
Using the racemic title compound of Preparative Example 6, Step C, and following essentially the same procedure as described above for Example 5, the racemate (Example 5B), is prepared, m.p. = 84.3°- 85.6°C; Mass Spec: MH+ = 607;
EXAMPLE 6
(-)-isomer
S ep A'*
Combine 3.21 g (6.80 mmol) of the (-)-isomer product of Preparative Example 6 and 150 mL of anhydrous DMF. Add 2.15 g (8.8 mmol) of 1-N-t-butoxycarbonylpiperidinyl-4-acetic acid, 1.69 g (8.8 mmol) of DEC, 1.19 g (8.8 mmol) of HOBT and 0.97 mL (8.8 mmol) of N- methylmorpholine and stir the mixture at room temperature overnight. Concentrate in vacuo to remove the DMF and add 50 mL of saturated NaHCθ3 (aqueous). Extract with CH2CI2 (2 X 250 mL), wash the extracts with 50 mL of brine and dry over MgS04* Concentrate in vacuo to a residue and chromatograph (silica gel, 2% MeOH/CH2Cl2 + 10% NH4OH) to give 4.75 g of the product, m.p. = 75.7°-78.5°C; Mass Spec: MH+ = 697; [a^5 = -5.5° (6.6 mg/2 mL MeOH). Step B:
Combine 4.70 g (6.74 mmol) of the product of Step A and 30 mL of MeOH, then add 50 mL of 10% H2Sθ4/dioxane in 10 mL aliquots over a 1 hr. period. Pour the mixture into 50 mL of water and add 15 mL of 50% NaOH (aqueous) to adjust to pH« 10-11. Fitter to remove the
resulting solids and extract the filtrate with CH2CI2 (2 X 250 mL). Concentrate the aqueous layer in vacuo to remove the MeOH and extract again with 250 L of CH2CI2. Dry the combined extracts over MgSθ4 and concentrate in vacuo to give the product, m.p. = 128.1 ' 1 13311..55°°CC; Mass Spec: MH+ = 597; [a] = -6.02° (9.3 mg/2 mL MeOH). Step C:
Combine 3.64 g (5.58 mmol) of the product of Step B and 30 mL of CH2CI2, then add 6.29 mL (44.64 mmol) of (CH3)3SiNCO and stir the mixture for 2 days at room temperature. Add 25 mL of NaHC03 (aqueous), then extract with CH2CI2 (2 X 250 mL). Wash the extracts with 25 mL of brine and dry over MgSθ4. Concentrate in vacuo to a residue and chromatograph (silica gel, gradient of 2.5%, 5.0%, then 7.5% MeOH/CH2CI2 + 10% NH4OH) to give the title compound, m.p. = 150.5°-153.0°C; Mass Spec: MH+ = 640; [a]D δ = -61.4° (8.18 mg/2 mL MeOH).
EXAMPLE 7
React the title compound of Preparative Example 7 and the title compound of Preparative Example 1 using substantially the same
procedure as described for Example 2, to give 0.25 g of the title compound, which is a racemic mixture of atropisomers. Mass Spec: MH+ = 604. m.p. = 167.2°-167.8°C.
The HCI salt of the title compound of Example 7 is prepared by stirring for 1 hr. with HCI/CH2CI2, then concentrating in vacuo Xo give the salt.
EXAMPLES 7A & 7B
The title compound of Example 7 is a racemic mixture of atropisomers. Those atropisomers are separated by preparative chromatography (HPLC), using an Chiralpack AD column (5 cm x 50 cm) and 40% i-PrOH/ hexane + 0.2% diethylamine as the mobile phase to give the (+)- and (-)-isomers, Examples 7B and 7A, respectively. Physical chemical data for (-)-isomer, Example 7A: m.p. = 114.2°-114.8°C; [a]^5 = -154.6° (8.73 mg/2 mL, MeOH).
Physical chemical data for (+)-isomer, Example 7B: m.p. = 112.6°-113.5°C; [a] = +159.7° (10.33 mg/2 mL, MeOH). EXAMPLE 8
React 6.0 g (12.8 mmol) of the title compound of Example 8 and with 3.78 g (16.6 mmol) of 1-N-t-butoxycarbonyl-piperidinyl-4-acetic acid using substantially the same procedures as described for Example 6, Step A, to give 8.52 g of the product. Mass Spec: MH+ = 694.0 (FAB). 1H NMR (CDCI3) 200 MHz): 8.5 (d, 1H); 7.5 (d, 2H); 7.2 (d, 1H); 4.15- 3.9 (m, 3H); 3.8-3.6 (m, 1H); 3.5-3.15 (m, 3H); 2.9 (d, 2H); 2.8-2.5 (m, 4H); 2.4-1.8 (m, 6H); 1.8-1.6 (br d, 2H); 1.4 (s, 9H); 1.25-1.0 (m, 2H).
Step B:
Combine 8.50 g of the product of Step A and 60 mL of CH2CI2, then cool to 0°C and add 55 L of TFA. Stir the mixture for 3 h at 0°C, then add 500 L of 1 Ν ΝaOH (aqueous) followed by 30 mL of 50% ΝaOH (aqueous). Extract with CH2C.2, dry over MgS04 and concentrate in vacuo to give 7.86 g of the product. Mass Spec: M+ = 593.9 (FAB). 1H ΝMR (CDCI3, 200 MHz): 8.51 (d, 1H); 7.52 (d of d, 2H); 7.20 (d, 1H); 4.1 -3.95 (m, 2H); 3.8-3.65 (m, 2H); 3.5-3.05 (m, 5H); 3.0- 2.5 (m, 6H); 2.45-1.6 (m, 6H);1.4-1.1 (m, 2H).
Step C:
Treat 7.80 g (13.1 mmol) of the product of Step B with 12.1 g (105 mmol) of (CH3)3SiNCO using substantially the same procedure as described for Example 6, Step C, to give 5.50 g of the title compound, which is a racemic mixture of atropisomers. m.p. = 163.6°-164.0°C. Mass spec: M+ = 636.9 (FAB). 1H NMR (CDCI3. 200 MHz): 8.5 (d, 1H); 7.52 (d, 1H); 7.48 (d, 1 H); 7.21 (d, 1H); 4.54, (s, 2H); 4.1-3.6 (m, 4H); 3.45-3.15 (m, 4H); 3.0-2.5 (m, 5H); 2.45-1.6 (m, 7H); 1.4-1.0, (m, 2H).
EXAMPLES 8A & 8B
Example 8A Example 8B
The title compound of Example 8 is a racemic mixture of atropisomers. Those atropisomers are separated by preparative chromatography (HPLC), using an Chiralpack AD column (5 cm x 50 cm) and 20% i-PrOH/ hexane + 0.2% diethylamine as the mobile phase, at a
flow rate of 100 mL min., to give the (+)- and (-)-isomers, Examples 8B and 8A, respectively.
Physical chemical data for (-)-isomer, Example 8A: m.p. = 142.9°-143.5°C; a]^ = -151.7° (11.06 mg/2 mL, MeOH).
Physical chemical data for (+)-isomer, Example 8B: m.p. = 126.5°-127.0°C; [a^5 = +145.6° (8.38 mg/2 mL, MeOH).
EXAMPLE 9
Combine 3.32 g of the (+)-isomer of the title compound of Preparative Example 8, Step B, 2.38 g of the title compound of
Preparative Example 1 , 1.92 g of HOBT, 2.70 g of DEC, 1.56 mL of N- methylmorpholine and 50 mL of dry DMF and stir at 25°C for 24 hrs. Concentrate in vacuo, then dilute the residue with CH2CI2. Wash with 1 N NaOH (aqueous), then with saturated Na2HP04 (aqueous) and dry over MgS04* Concentrate in vacuo to a residue and chromatograph
(silica gel, 2% Me0H/CH2Cl2 + NH4OH) to give the crude product. Rechromatograph using 1% MeOH/CH2C*2 + NH4OH to give 3.82 g of the title compound.
The HCI salt of the title compound of Example 9 is prepared by dissolving of the title compound in CH2CI2, adding 6M HCI (g) in
CH2CI2, then concentrating in vacuo Xo give the salt. m.p. = 166.5°C;
[a]o5 = +70.8° (MeOH).
EXAMPLES 9A & 9B
Example 9A Example 9B The (-)-isomer of the title compound of Preparative Example 8,
Step B, (3.38 g) is reacted with 2.20 g of the title compound of Preparative Example 1 , via substantially the same procedure as described for Example 9 to give 3.58 g of the title compound Example 9A. The HCI salt of the title compound of Example 9A is prepared by dissolving of the title compound in CH2CI2, adding 6M HCI (g) in
,25 CH2CI2, then concentrating in vacuo to give the salt. m.p. = 129°C; [a]D ; = -72.3° (MeOH).
The racemic title compound of Preparative Example 8, Step A, is reacted with the title compound of Preparative Example 1 , via substantially the same procedure as described for Example 9 to give the title compound Example 9B. m.p. = 145.0°C
EXAMPLE 10
React 1.33 g of the (+)-isomer of the title compound of Preparative Example 8, Step B, with 1.37 g of 1-N-t-butoxy-carbonylpiperidinyl-4- acetic acid using substantially the same procedures as described for Example 6, Step A, to give 2.78 g of the product. Mass Spec: MH+ = 694.0 (FAB); [a] 5 = +34.1° ( mg/2 L, MeOH). Step B:
Treat 2.78 g of the product of Step A via substantially the same procedure as described for Example 8, Step B, to give 1.72 g of the product, m.p. = 104.1°C; Mass Spec: M+ = 597; [a]^ = +53.4° ( mg 2 mL, MeOH).
Treat 1.58 g of the product of Step B with 6 mL of (CH3)3SiNCO using substantially the same procedure as described for Example 6, Step C, to give 1.40 g of the title compound, m.p. = 140°C; Mass spec M+ = 639; [a]o5 = +49.1° ( mg/2 mL, MeOH).
EXAMPLES 10A & 1 OB
Example 10A Example 10B
The (-)-isomer of the title compound of Preparative Example 8, Step B, (3.38 g) is converted to the title compound (Example 9A) via substantially the same procedure as described for Example 10, Steps A- C, to give the title compound Example 9A. m.p. = 152°C; Mass spec: M+ = 606; [ajo5 = -62.5° (MeOH).
The racemic title compound of Preparative Example 8, Step A, is converted to the title compound (Example 9B) via substantially the same procedure as described for Example 10, Steps A-C to give the title compound Example 9B. m.p. = 111.2°C (dec).
Ex-ample 11
The title compound is prepared using the racemic title compound from Preparative Example 9, Step F, following substantially the same procedure as described for Example 2.
1H NMR (CDCI3. 400 MHz): 8.44 (d, 1H); 8.14 (d, 2H): 7.58 (d, 1 H); 7.47 (d, 1H); 7.14 (m, 3H); 5.32 (s, 1H); 4.65-4.57 (m, 1H); 3.68 (s, 2H); 3.65-3.39 (m, 4H); 2.91-2.87 (m, 1H); 2.69-2.63 (m, 1H); 2.45-2.33 (m,
4H).
Example 11A & 11B
Example 11A Example 11 B
Using the R(+)- or S(-)-isomer of the title compound from Preparative Example 9, Step G, the R(+)-isomer (Example 11A) or the S- (-)-isomer (Example 11 B) is prepared using substantially the same procedure as described for Example 2.
Physical chemical data for R-(+)-isomer, Example 11 A: m.p. = 167.0°-167.8°C; [a] = +32.6° (MeOH); 1H NMR (CDCI3, 400 MHz): 8.44 (d, 1H); 8.14 (d, 2H): 7.58 (d, 1H); 7.47 (d, 1H); 7.14 (m, 3H); 5.32 (s, 1 H); 4.65-4.57 (m, 1H); 3.68 (s, 2H); 3.65-3.39 (m, 4H); 2.91-2.87 (m, 1 H); 2.69-2.63 (m, 1H); 2.45-2.33 (m, 4H).
Physical chemical data for S-(-)-isomer, Example 11B: [a]o5 = -38.2° (14.67 mg/2 mL, MeOH); 1H NMR (CDCI3, 400 MHz): 8.44 (d, 1 H); 8.14 (d, 2H): 7.58 (d, 1H); 7.47 (d, 1H); 7.14 (m. 3H); 5.32 (s, 1H); 4.64-4.57 (m, 1 H); 3.67 (s, 2H); 3.70-3.34 (m, 4H); 2.95-2.87 (m, 1H); 2.69-2.63 (m, 1 H); 2.45-2.31 (m, 4H).
Example 12
The title compound of this Example is prepared using the racemic title compound from Preparative Example 9, Step F, by following substantially the same procedures as described for Example 8, Steps A- C. This compound is a racemate.
EXAMPLES 12A & 12B
Example 12A Example 12B
The title compound of Example 12 is a racemic mixture of enantiomers. Chromatograph 2.45 g of the compound of Example 12, using an Chiralpack AD column and 20% i-PrOH/ hexane + 0.2% diethylamine as the mobile phase, at a flow rate of 100 mL/min., to give 0.970 g of the (+)-isomer and 0.982 g of the (-)-isomer, Examples 12B and 12A, respectively. Physical chemical data for (-)-isomer, Example 12A: H NMR (CDCI3, 200 MHz): 8.43 (d, 1H); 7.58 (d, 1H); 7.48 (d, 1 H); 7.14 (d, 1 H); 5.32 (s, 1H); 4.5-4.75 (m, 1H); 4.4 (s, 2H); 3.9 (d, 2H); 3.2-3.7 (m, 5H); 2.52-3.05 (m, 4H); 1.85-2.5 (m, 6H); 1.5-1.85 (m, 4H); 1.0-1.4 (m, 1 H). [a]p5 = -31.2° (MeOH). Physical chemical data for (+)-isomer, Example 12B:
1H NMR (CDCI3, 200 MHz): 8.43 (d, 1H); 7.58 (d, 1H); 7.48 (d, 1 H);
7.14 (d, 1 H); 5.32 (s, 1 H); 4.5-4.75 (m, 1 H); 4.4 (s, 2H); 3.9 (d, 2H);
3.2-3.7 (m, 5H); 2.52-3.05 (m, 4H); 1.85-2.5 (m, 6H); 1.5-1.85 (m, 4H);
1.0-1.4 (m, 1 H). [a]o5 = +29.8° (MeOH).
EXAMPLE 13
Step A:
React 1.35 g of the (-)-isomer of the title compound of Preparative Example 10, Step B, with 1.4 g of 1-N-t-butoxy-carbonylpiperidinyl-4- acetic acid following substantially the same procedures as described for Example 6, Step A, to give 2.0 g of the product. Mass Spec: MH+ = 696 (FAB), partial 1H NMR (CDCI3, 300 MHz): 8.38 (s, 1H); 7.60 (s, 1H); 7.25 (d, 1H); 7.05 (m, 1H); 1.45 (s, 9H).
Step B:
Treat 1.95 g of the product of Step A via substantially the same procedure as described for Example 8, Step B, to give 1.63 g of the product. Mass Spec. MH+ = 596 (FAB), partial 1H NMR (CDCI3, 300 MHz): 8.38 (s, 1 H); 7.60 (s, 1 H); 7.25 (d, 1H); 7.03 (m, 1H); 4.64 (d, 1 H); 3.90 (m, 2H).
Step C:
(-)-isomer
Treat 1.6 g of the product of Step B with 1.3 mL of (CH3)3SiNCO using substantially the same procedure as described for Example 6, Step C, to give 1.27 g of the title compound. Mass spec: M+ = 639 (FAB); [a]o5 = -33.1 ° (c=0.58, EtOH). partial 1H NMR (CDCI3, 400 MHz): 8.38 (s, 1H); 7.59 (s, 1 H); 7.25 (d, 1H); 7.04 (m, 1H); 4.60 (d, 1H); 4.41 (s, 2H).
EXAMPLES 13A & 13B
The (+)-isomer of the title compound from Preparative Example 10, Step B, (2.1 g) is converted to the title compound via substantially the same procedure as described for Example 10, Steps A-C, to give the title compound, Example 13A. Mass spec: MH+ = 638.9; [a]p5 = +32.4° (c=0.57, EtOH). partial 1H NMR (CDCI3, 400 MHz): 8.39 (s, 1H); 7.59
(s, 1H); 7.25 (d, 1H); 7.04 (m, 1H); 4.60 (d, 1H); 4.41 (s, 2H). partial H
NMR (DMSO-d6, 400 MHz): 8.42 (s, 1H); 7.88 (s, 1H); 7.41 (d, 1H);
7.29 (m, 1H); 5.85 (s, 2H); 4.20 (d, 1H).
The racemic title compound from Preparative Example 10, Step A, is converted to the racemic title compound, Example 13B, in an analogous manner, partial 1H NMR (CDCI3, 400 MHz): 8.38 (s, 1H);
7.59 (s, 1H); 7.25 (d, 1 H); 7.04 (m, 1 H); 4.60 (d, 1H); 4.41 (s, 2H). partial H NMR (DMSO-d6, 400 MHz): 8.42 (s, 1H); 7.88 (s, 1H); 7.41
(d, 1H); 7.29 (d, 1 H); 5.85 (s, 2H); 4.20 (d, 1H).
EXAMPLE 14
React 2.6 g of the (+)-isomer of the title compound of Preparative Example 10, Step B, and 1.68 g of the title compound of Preparative Example 1 following substantially the same procedure as described for Example 9 to give 2.10 g of the title compound. Mass spec: MH+ = 606 (FAB); [a]o5 = +34.1 ° (10.98 mg/2 mL, EtOH). partial 1 H NMR (CDCI3, 400 MHz): 8.38 (s, 1 H); 8.15 (d, 2H); 7.58 (s, 1H); 7.26 (d, 1H); 7.15 (d, 2H); 7.03 (d, 1 H); 4.57 (d, 1H).
To prepared the HCI salt of the title compound of Example 14 dissolve 700 mg of the title compound in 4 mL of CH2CI2, add 4 mL of Et2θ, cool to 0°C and slowly add (dropwise) 1 mL of HCI (g) in dioxane. Add 2 mL of Et2θ and stir at 0°C for 7 min. Dilute with 30 mL of Et2θ, filter to collect the solid product and wash with 30 mL of E_2θ. Dry the solids in vacuo to give 0.836 g of the HCI salt of Example 14. [a]D 5 = +64.8° (9.94 mg/2 mL, EtOH).
EXAMPLE 14A & 14B
Example 14A Example 14B
The (-)-isomer of the title compound of Preparative Example 10, Step B, (0.60 g) is reacted with 0.39 g of the title compound of Preparative Example 1 , via substantially the same procedure as described for Example 9 to give 0.705 g of the title compound. Mass spec: MH+ = 604 (FAB); [a]o5 = -41.8° (EtOH). partial ■ H NMR (CDCI3, 300 MHz): 8.38 (s, 1H); 8.15 (d, 2H); 7.58 (s, 1H); 7.26 (d, 1H); 7.15 (d, 2H); 7.03 (d, 1H); 4.57 (d, 1 H).
The HCI salt of the title compound of Example 14A is prepared via
25 substantially the same procedure as described for Example 14. [a]D = -63.2° (EtOH).
The racemic title compound of Preparative Example 10, Step A, is converted to the racemic title compound of Example 14B following substantially the same procedure as described for Example 9. partial 1H NMR (CDCI3. 400 MHz): 8.38 (s, 1 H); 8.15 (d, 2H); 7.58 (s, 1H); 7.26 (d, 1H); 7.15 (d, 2H); 7.03 (d, 1H); 4.57 (d, 1H). partial H NMR
(DMSO-d6, 400 MHz): 8.77 (d, 2H); 8.47 (s, 1H); 7.95 (s, 1H); 7.74 (d, 2H); 7.43 (m, 1H); 7.27 (d, 1H); 4.35 (d, 1H).
EXAMPLE 15
The title compound of Preparative EΞxample 4 is reacted via substantially the same methods as described for Example 8, Steps A-C, to give the title compound, which is a racemate. Mass Spec: MH+ = 637 (FAB), partial H NMR (CDCI3): 8.45 (s, 1H); 7.60 (s, 1H); 7.35 (d, 1H); 7.05 (d, 1 H); 4.45 (s, 1 H).
Example 16A & 16B
Example 16A Example 16B The R-(+)-isomer or the S-(-) isomer of the title comound of
Preparative Example 11 is treated via substantially the same procedure as described for Example 2 to give the R-(+)-isomer of the title compound or the S-(-)-isomer of the title compound, Examples 16A and
16B, respectively. Physical chemical data for the R-(+)-isomer: 13C NMR (CDCI3):
166.5 (C); 154.8 (C); 146.6 (CH); 140.8 (CH); 140.4 (C); 138.5 (CH);
138.5 (CH); 136.3 (C); 134.6 (C); 133.8 (C); 133.6 (C); 132.0 (CH);
130.0 (CH); 126.3 (CH); 126.3 (CH); 125.8 (CH); 119.6 (C); 78.4 (CH);
51.1 (CH2); 50.6 (CH2); 45.4 (CH); 41.5 (CH ); 38.0 (CH2); 30.1 (CH2); 30.0 (CH2). [a]o5 = +30.7° (10.35 mg/2 mL MeOH).
Physical chemical data for the S-(-)-isomer: 13C NMR (CDCI3):
166.5 (C); 154.8 (C); 146.6 (CH); 140.8 (CH); 140.4 (C); 138.5 (CH);
138.5 (CH); 136.3 (C); 134.6 (C); 133.8 (C); 133.6 (C); 132.0 (CH);
130.0 (CH); 126.3 (CH); 126.3 (CH); 125.8 (CH); 119.6 (C); 78.4 (CH); 51.1 (CH2); 50.6 (CH2); 45.4 (CH); 41.5 (CH2); 38.0 (CH2); 30.1
(CH2); 29.9 (CH2). [a]p5 = -30.9° (9.70 mg 2 mL MeOH).
Examples 17 & 17A
Treat the (+)-isomer or the (-)-isomer of the title compound of Preparative Example 11 via substantially the same procedure as described for Example 1 , Steps A-C, to give the R-(+)-isomer of the title compound or the S-(-)-isomer of the title compound, Examples 17 and 17A, respectively.
Physical chemical data for the R-(+)-isomer: 13C NMR (CDCI3): 169.3 (C); 157.5 (C); 155.0 (C); 146.6 (CH); 140.8 (CH); 140.4 (C); 136.3 (C); 134.8 (C); 133.7 (C); 132.0 (CH); 130.0 (CH); 125.8 (CH); 119.6 (C); 78.5 (CH); 51.4 (CH2); 50.9 (CH2); 45.2 (CH); 43.9 (CH2); 43.9 (CH ). 41.1 (CH2); 38.8 (CH2); 32.5 (CH); 31.5 (CH2); 31.5 CH2); 30.1 (CH2); 30.0 (CH2). [a ,48 = +28.7° (10.1 mg 2 L MeOH). Physical chemical data for the S-(-)-isomer: 13C NMR (CDCI3): 169.3 (C); 157.6 (C); 155.0 (C); 146.6 (CH); 140.8 (CH); 140.4 (C); 136.3 (C); 134.8 (C); 133.7 (C); 132.0 (CH); 130.0 (CH); 125.8 (CH); 119.6 (C); 78.5 (CH); 51.4 (CH2); 50.9 (CH2); 45.2 (CH); 43.9 (CH2);
43.9 (CH2); 41.1 (CH2); 38.8 (CH2); 32.5 (CH); 31.5 (CH2); 31.5
,24.8
(CH2); 30.1 (CH2); 30.0 (CH2). [a£ = -28.5° (10.1 mg/2 mL MeOH)
Dissolve 9.90 g (18.9 mmol) of the product of Preparative Example 7, Step B, in 150 mL CH2CI2 and 200 mL of CH3CN and heat to 60°C. Add 2.77 g (20.8 mmol) N-chlorosuccinimide and heat to reflux for 3 h., monitoring the reaction by TCL (30%EtOAc/H2θ). Add an additional 2.35 g (10.4 mmol) of N-chlorosuccinimide and reflux an additional 45 min. Cool the reaction mixture to room temperature and extract with 1 N NaOH and CH2CI2. Dry the CH2CI2 layer over MgSθ4, filter and purify by flash chromatography (1200 mL normal phase silica gel, eluting with 30% EtOAc/H2θ) to obtain 6.24 g of the desired product. M.p. 193-195.4°C.
To 160 mL of cone HCI at -10°C add 2.07 g (30.1 mmol) NaN02 and stir for 10 min. Add 5.18 g (10.1 mmol) of the product of Step A and warm the reaction mixture from -10°C to 0°C for 2 h. Cool the reaction to -10°C, add 100 mL H3PO2 and let stand ovemight. To extract the reaction mixture, pour over crushed ice and basifiy with 50% NaOH/ CH2CI2. Dry the organic layer over MgS04, filter and concentrate to dryness. Purify by flash chromatography (600 mL normal phase silica gel, eluting with 20% EtOAc/hexane) to obtain 3.98 g of product. Mass spec: MH+=497.2.
Step C:
Dissolve 3.9 g of the product of Step B in 100 mL cone. HCI and reflux ovemight. Cool the mixture, basify with 50 % w/w NaOH and extract the resultant mixture with CH2CI2. Dry the CH2CI2 layer over MgS04, evaporate the solvent and dry under vacuum to obtain 3.09 g of the desired product. Mass spec: MH+=424.9.
Using a procedure similar to that described in Preparative Example 8, obtain 1.73 g of the desired product, m.p. 169.6-170.1 °C; [a]o5 = +48.2° (c=1 , MeOH).
Step E:
Use a procedure similar to that of Example 4 with the product of Step D as the starting material to obtain the title compound. M.p. 152.3- 153.3°C; [a] = +53.0° (c=1, MeOH).
EXAMPLE 1
4-[2-[4-(3-BROMO-8-CHLORO-6,11 -DIHYDRO-5H-BENZO [5,6]CYCLOHEPTA[1 ,2-b]PYRIDIN-11-YL)-1-PIPERAZINYL]-2-
OXOETHYLJ-1 - PIPERIDINECARBOXAMIDE
Procedure i:
1-(3-Bromo-8-chloro-6,11-dihydro-5H- benzo[5,6]cyclohepta[1 ,2-b]pyridin-11 -yl)-4-[(4- piperidinyl)acetyl]piperazine (1 equivalent) (0.3g) and urea (Aldrich 99%)
(10 equivalents) (0.3479g) were slurried in distilled water (3ml) and the heterogeneous mixture was heated under reflux at 100°C for 66h with stirring. The mixture was dissolved in methanol/dichloromethane and then evaporated to dryness. The product was chromatographed on a silica gel column (15x2.5cm) using 3%(10% concentrated ammonium hydroxide in methanoi)-dichloromethane as the eluant to give the title compound (0.2863g.; 88%), FABMS: m/z 562.1 (MH+).
dc (CDCI3)
Tricyclic CH2: 30.5, 30.6
CH: 147.1 , 141.4, 132.5, 126.3, 130.6, 79.1
C: 120.1 , 140.9, 134.3, 135.2, 136.9, 155.4
Piperazine CH2: 41.6, 45.7, 51.5, 51.9
Piperazine CH2: 44.4, 44.5, 32.1 , 32.1 , 39.3 N-substituent CH: 33.0
C: 169.8, 158.1
Procedure (ii):
1 -(3-Bromo-8-chloro-6, 11 -dihydro-5H- benzo[5,6]cyclohepta[1 ,2-b]pyridin-11 -yl)-4-[(4- piperidinyl)acetyl]piperazine (1 equivalent) (0.3g) and urea (Aldrich 99%) (4 equivalents) (0.1392g) were slurried in distilled water (12ml) and the mixture was heated under reflux at 100°C for 3h with stirring. The mixture was dissolved in methanol/dichloromethane and then evaporated to dryness. The product was chromatographed on a silica gel column (15x2.5cm) using 3% increasing to 7%(10% concentrated ammonium hydroxide in methanol)-dichloromethane as the eluant to give the title compound (0.1486g.; 46%), which was identical to that prepared in Procedure (i) above.
Procedure (iii):
1 -(3-Bromo-8-chloro-6, 11 -dihydro-5H- benzo[5,6]cyclohepta[1 ,2-b]pyridin-11-yl)-4-[(4- piperidinyl)acetyl]piperazine (1 equivalent) (0.3g) and urea (Aldrich 99%) (4 equivalents) (0.1392g) were slurried in distilled water (3ml) and the mixture was heated under reflux at 100°C for 18h with stirring. The mixture was dissolved in methanol/dichloromethane and then evaporated to dryness. The product was chromatographed on a silica gel column (15x2.5cm) using 3% increasing to 7%(10% concentrated
ammonium hydroxide in methanol)-dichIoromethane as the eluant to give the title compound (0.2163g.; 67%), which was identical to that prepared in Procedures (i) and (ii) above.
EXAMPLE 2
(+)-4-[2-[4-(8-CHLORO-3,10-DIBROMO-6,11-D_HYDRO-5H-
BENZO[5,6]CYCLOHEPTA[1 ,2-b]PYRIDIN-11 (R)-YL)-1- PIPERIDINYL]-2-OXOETHYL]-1-PIPERIDINECARBOXAMIDE
(+)-4-{8-Chloro-3,10-dibromo-6,11-dihydro-5H- benzo[5,6lcycloheρta[1 ,2-b]pyridin-11 (R)-yl)-1-(4- piperidinylacetyl)piperidine (1 equivalent) (0.060g) and urea (Aldrich 99%) (10 equivalents) (0.065g) were slurried in distilled water (1ml) and the mixture was heated under reflux at 100°C for 68h with stirring, the volume being maintained at 1 ml throughout the reaction by the addition of water as needed. The mixture was dissolved in methanol/dichloromethane and then evaporated to dryness. The product was chromatographed on a silica gel column (15x1 cm) using 3%(10% concentrated ammonium hydroxide in methanol)-dichloromethane as
the eluant to give the title compound (0.0632g; 98%), FABMS: m/z 639.4 (MH+).
dc (CDCI3)
Tricyclic CH2: 30.2, 30.2
CH: 147.5/147.3, 141.5/141.7, 131.1/131.2, 129.2/129.3, 58.1
C: 119.0, 142.6/142.7, 135.2/135.4, 133.2/133.3, 127.2/127.3, 137.0/137.3, 154.7/154.8
Piperidine CH2: 45.9/45.8, 42.0/41.8, 31.2/31.3, 31.9
CH: 42.0/42.2
Piperidine CH2: 44.4/44.6, 44.4/44.6, 32.1/32.2, 32.1/32.2, 39.4
N-substituent CH: 33.0
C: 169.7, 158.0