WO1997046712A3 - System and method for carrying out and monitoring biological processes - Google Patents

System and method for carrying out and monitoring biological processes Download PDF

Info

Publication number
WO1997046712A3
WO1997046712A3 PCT/US1997/009856 US9709856W WO9746712A3 WO 1997046712 A3 WO1997046712 A3 WO 1997046712A3 US 9709856 W US9709856 W US 9709856W WO 9746712 A3 WO9746712 A3 WO 9746712A3
Authority
WO
WIPO (PCT)
Prior art keywords
sample chamber
monitoring
per cycle
dna
carrying
Prior art date
Application number
PCT/US1997/009856
Other languages
French (fr)
Other versions
WO1997046712A2 (en
Inventor
Carl T Wittwer
Kirk M Ririe
Randy P Rasmussen
David R Hillyard
Original Assignee
Univ Utah Res Found
Carl T Wittwer
Kirk M Ririe
Randy P Rasmussen
David R Hillyard
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24643601&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO1997046712(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ Utah Res Found, Carl T Wittwer, Kirk M Ririe, Randy P Rasmussen, David R Hillyard filed Critical Univ Utah Res Found
Priority to JP50087498A priority Critical patent/JP4281877B2/en
Priority to DE69727932T priority patent/DE69727932T2/en
Priority to EP10174616.2A priority patent/EP2298931B2/en
Priority to AT97929833T priority patent/ATE260988T1/en
Priority to CA2256773A priority patent/CA2256773C/en
Priority to NZ333137A priority patent/NZ333137A/en
Priority to AU33800/97A priority patent/AU729644B2/en
Priority to EP97929833A priority patent/EP0906449B1/en
Publication of WO1997046712A2 publication Critical patent/WO1997046712A2/en
Publication of WO1997046712A3 publication Critical patent/WO1997046712A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B01L2300/1844—Means for temperature control using fluid heat transfer medium using fans
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6482—Sample cells, cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178—Special arrangements of analysers
    • G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/07—Centrifugal type cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Optical Measuring Cells (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Selective Calling Equipment (AREA)
  • Microscoopes, Condenser (AREA)
  • Control Of Temperature (AREA)

Abstract

A thermal cycling method and device is disclosed. The device comprises a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such as the DNA polymerase chain reaction. Biological samples are placed in glass micro capillary tubes and then located inside the sample chamber. A programmable controller regulates the temperature of the sample inside the sample chamber. Monitoring of the DNA amplification is monitored by fluorescence once per cycle or many times per cycle. The present invention provides that fluorescence monitoring of PCR is a powerful tool for DNA quantification.
PCT/US1997/009856 1996-06-04 1997-06-04 System and method for carrying out and monitoring biological processes WO1997046712A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
JP50087498A JP4281877B2 (en) 1996-06-04 1997-06-04 System and method for performing and monitoring biological processes
DE69727932T DE69727932T2 (en) 1996-06-04 1997-06-04 DEVICE AND METHOD FOR PERFORMING AND MONITORING POLYMERASE CHAIN REACTIONS
EP10174616.2A EP2298931B2 (en) 1996-06-04 1997-06-04 Apparatus for performing PCR and monitoring the reaction in real time during temperature cycling
AT97929833T ATE260988T1 (en) 1996-06-04 1997-06-04 DEVICE AND METHOD FOR CARRYING OUT AND MONITORING POLYMERASE CHAIN REACTIONS
CA2256773A CA2256773C (en) 1996-06-04 1997-06-04 System and process for carrying out and monitoring polymerase chain reaction
NZ333137A NZ333137A (en) 1996-06-04 1997-06-04 System and method for carrying out thermal cycling for biological processes such as the polymerase chain reaction
AU33800/97A AU729644B2 (en) 1996-06-04 1997-06-04 System and method for carrying out and monitoring biological processes
EP97929833A EP0906449B1 (en) 1996-06-04 1997-06-04 System and method for carrying out and monitoring polymerase chain reactions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US65899396A 1996-06-04 1996-06-04
US08/658,993 1996-06-04

Publications (2)

Publication Number Publication Date
WO1997046712A2 WO1997046712A2 (en) 1997-12-11
WO1997046712A3 true WO1997046712A3 (en) 1998-05-07

Family

ID=24643601

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US1997/009620 WO1997046707A2 (en) 1996-06-04 1997-06-04 System and method for monitoring for dna amplification by fluorescence
PCT/US1997/009856 WO1997046712A2 (en) 1996-06-04 1997-06-04 System and method for carrying out and monitoring biological processes

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US1997/009620 WO1997046707A2 (en) 1996-06-04 1997-06-04 System and method for monitoring for dna amplification by fluorescence

Country Status (11)

Country Link
EP (7) EP0912760B1 (en)
JP (2) JP4278712B2 (en)
AT (5) ATE321891T1 (en)
AU (2) AU727296B2 (en)
CA (2) CA2256773C (en)
DE (5) DE69727932T2 (en)
DK (1) DK2298931T3 (en)
ES (6) ES2260562T3 (en)
NZ (2) NZ333137A (en)
PT (1) PT2298931E (en)
WO (2) WO1997046707A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8623602B2 (en) 2008-08-01 2014-01-07 Roche Diagnostics Operations, Inc. Lysis and reverse transcription for MRNA quantification
US9416416B2 (en) 2007-10-01 2016-08-16 Longhorn Vaccines And Diagnostics, Llc Biological specimen collection/transport compositions and methods
US9481912B2 (en) 2006-09-12 2016-11-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and identifying nucleic acid sequences in biological samples

Families Citing this family (235)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2333520T3 (en) * 1997-02-28 2014-09-29 Cepheid Heat-exchanging, requested chemical reaction device
DE19811730A1 (en) * 1998-03-18 1999-09-23 November Ag Molekulare Medizin Identifying marker that indicates presence of immobilized nucleic acid using fluorophore-labeled detection agent bound to solid phase
ATE363339T1 (en) 1998-05-01 2007-06-15 Gen Probe Inc STIRRING DEVICE FOR THE FLUID CONTENTS OF A CONTAINER
US7498164B2 (en) 1998-05-16 2009-03-03 Applied Biosystems, Llc Instrument for monitoring nucleic acid sequence amplification reaction
US6818437B1 (en) 1998-05-16 2004-11-16 Applera Corporation Instrument for monitoring polymerase chain reaction of DNA
CN1664562A (en) 1998-05-16 2005-09-07 阿普尔拉公司 Instrument for monitoring polymerase chain reaction of DNA
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
DE19850593A1 (en) * 1998-11-03 2000-05-04 Biochip Technologies Gmbh DNA hybridization assay comprises detecting fluorescent resonance energy transfer between fluorochromes on different DNA strands
US6303305B1 (en) 1999-03-30 2001-10-16 Roche Diagnostics, Gmbh Method for quantification of an analyte
US6387621B1 (en) 1999-04-27 2002-05-14 University Of Utah Research Foundation Automated analysis of real-time nucleic acid amplification
US20050279949A1 (en) 1999-05-17 2005-12-22 Applera Corporation Temperature control for light-emitting diode stabilization
US7387891B2 (en) 1999-05-17 2008-06-17 Applera Corporation Optical instrument including excitation source
US7410793B2 (en) 1999-05-17 2008-08-12 Applera Corporation Optical instrument including excitation source
US7423750B2 (en) 2001-11-29 2008-09-09 Applera Corporation Configurations, systems, and methods for optical scanning with at least one first relative angular motion and at least one second angular motion or at least one linear motion
EP1274986B1 (en) 1999-08-13 2011-07-13 Bayer Technology Services GmbH Device and method for determining multiple analytes
DE19946296A1 (en) * 1999-09-28 2001-03-29 Roche Diagnostics Gmbh Multiplex PCR for the detection of EHEC infections
WO2001035079A1 (en) * 1999-11-12 2001-05-17 E. I. Du Pont De Nemours And Company Fluorometer with low heat-generating light source
US6691041B2 (en) 2000-03-31 2004-02-10 Roche Molecular Systems, Inc. Method for the efficiency-corrected real-time quantification of nucleic acids
DE10045521A1 (en) 2000-03-31 2001-10-04 Roche Diagnostics Gmbh Determining amplification efficiency of a target nucleic acid comprises measuring real-time amplification of diluted series, setting signal threshold value, and determining cycle number at which threshold is exceeded
US6783934B1 (en) 2000-05-01 2004-08-31 Cepheid, Inc. Methods for quantitative analysis of nucleic acid amplification reaction
US6720187B2 (en) 2000-06-28 2004-04-13 3M Innovative Properties Company Multi-format sample processing devices
AU7024801A (en) * 2000-06-28 2002-01-08 3M Innovative Properties Co Enhanced sample processing devices, systems and methods
US6734401B2 (en) 2000-06-28 2004-05-11 3M Innovative Properties Company Enhanced sample processing devices, systems and methods
US6563581B1 (en) 2000-07-14 2003-05-13 Applera Corporation Scanning system and method for scanning a plurality of samples
AU2001296864A1 (en) 2000-08-31 2002-03-13 Mayo Foundation For Medical Education And Research Detection of varicella-zoster virus
DE10044373A1 (en) 2000-09-08 2002-03-21 Roche Diagnostics Gmbh New reagent for labeling nucleic acids
WO2002061390A2 (en) 2001-01-31 2002-08-08 Mayo Foundation For Medical Education And Research Detection of herpes simplex virus
DE60228952D1 (en) 2001-05-07 2008-10-30 Mayo Foundation Detection of Legionella by PCR and FRET using method
US7226732B2 (en) * 2001-07-16 2007-06-05 Cepheid Methods, apparatus, and computer programs for verifying the integrity of a probe
US7635588B2 (en) 2001-11-29 2009-12-22 Applied Biosystems, Llc Apparatus and method for differentiating multiple fluorescence signals by excitation wavelength
US6889468B2 (en) 2001-12-28 2005-05-10 3M Innovative Properties Company Modular systems and methods for using sample processing devices
US7373253B2 (en) 2002-02-12 2008-05-13 Idaho Technology Multi-test analysis of real-time nucleic acid amplification
US6593093B1 (en) 2002-02-20 2003-07-15 Mayo Foundation For Medical Education And Research Detection of group a Streptococcus
US7785776B2 (en) 2002-05-13 2010-08-31 Idaho Technology, Inc. Genotyping by amplicon melting curve analysis
ATE537438T1 (en) 2002-05-17 2011-12-15 Life Technologies Corp DEVICE AND METHOD FOR DISTINGUISHING SEVERAL FLUORESCENCE SIGNALS BASED ON THEIR EXCITATION WAVELENGTH
AUPS267802A0 (en) * 2002-05-30 2002-06-20 Bio-Molecular Holdings Pty Limited Improved dna amplification apparatus and method
CA2433473A1 (en) * 2002-07-23 2004-01-23 F. Hoffmann-La Roche Ag Fluorescent hybridization probes with reduced background
US7074598B2 (en) 2002-09-25 2006-07-11 Mayo Foundation For Medical Education And Research Detection of vancomycin-resistant enterococcus spp.
US7365176B2 (en) 2002-09-26 2008-04-29 Mayo Foundation For Medical Education And Research Detection of Epstein-Barr virus
US7074599B2 (en) 2002-09-27 2006-07-11 Mayo Foundation For Medical Education And Research Detection of mecA-containing Staphylococcus spp.
US7582429B2 (en) 2002-10-23 2009-09-01 University Of Utah Research Foundation Amplicon melting analysis with saturation dyes
US9850381B2 (en) * 2002-10-23 2017-12-26 University Of Utah Research Foundation Amplicon melting analysis with saturation dyes
EP1564300A4 (en) * 2002-11-13 2007-09-05 Hitachi High Tech Corp Hethod of amplifying nucleic acid and apparatus therefor
US7718361B2 (en) 2002-12-06 2010-05-18 Roche Molecular Systems, Inc. Quantitative test for bacterial pathogens
DE60333480D1 (en) 2002-12-06 2010-09-02 Roche Diagnostics Gmbh METHOD FOR DETECTING PATHOGENIC ORGANISMS
GB2399776A (en) * 2003-03-24 2004-09-29 Pa Knowledge Ltd Cyclical heating and cooling device and associated methods
JP2006521092A (en) * 2003-04-04 2006-09-21 エフ．ホフマン−ラ　ロシュ　アーゲー Improved system for multicolor real-time PCR
US7148043B2 (en) 2003-05-08 2006-12-12 Bio-Rad Laboratories, Inc. Systems and methods for fluorescence detection with a movable detection module
US7788039B2 (en) 2003-09-25 2010-08-31 Roche Molecular Systems, Inc. Quantitation of nucleic acids using growth curves
CA2494571C (en) 2003-12-02 2010-02-09 F.Hoffmann-La Roche Ag Oligonucleotides containing molecular rods
EP1709200B1 (en) 2003-12-03 2012-05-09 Abbott Laboratories Double stranded linear nucleic acid probe and uses thereof
EP1753873A4 (en) 2004-03-05 2008-11-05 Ohio Med College Methods and compositions for assessing nucleic acids and alleles
JP2005295877A (en) * 2004-04-09 2005-10-27 Taiyo Yuden Co Ltd Method for analyzing nucleic acid, analyzer and disk for analysis
US7476502B2 (en) 2004-04-12 2009-01-13 Willey James C Methods and compositions for assaying analytes
US9657347B2 (en) 2004-04-20 2017-05-23 University of Utah Research Foundation and BioFire Defense, LLC Nucleic acid melting analysis with saturation dyes
TWI392863B (en) 2004-08-05 2013-04-11 Universal Bio Research Co Ltd Reaction vessel, liquid introduction device of reaction vessel, device for detecting the introduced reaction liquid, and liquid introudction device
ATE406462T1 (en) * 2004-08-18 2008-09-15 Hoffmann La Roche METHOD FOR MEASURING NUCLEIC ACID AMPLIFICATION IN REAL TIME INCLUDING THE POSITIONING OF A REACTION VESSEL RELATIVE TO A DETECTION UNIT
GB0419325D0 (en) * 2004-09-01 2004-09-29 Perkinelmer Ltd A method of analysing a sample including fluorescent labels and apparatus therefor
EP1634965B1 (en) 2004-09-09 2010-01-20 Roche Diagnostics GmbH Real time PCR with the addition of pyrophosphatase
DE102004043909A1 (en) * 2004-09-10 2006-03-30 Kendro Laboratory Products Gmbh Heating device for samples in the field of life science
EP1801196A4 (en) 2004-10-06 2011-06-15 Universal Bio Research Co Ltd Reaction container and reaction controller
US20060094028A1 (en) * 2004-11-04 2006-05-04 Welch Allyn, Inc. Rapid diagnostic assay
ES2285578T3 (en) 2005-01-18 2007-11-16 F. Hoffmann-La Roche Ag VISUALIZATION OF FLUORESCENCE SIGNS USING TELECENTRICITY.
ES2284087T3 (en) 2005-01-18 2007-11-01 F. Hoffmann-La Roche Ag GENERATION OF FLUORESCENCE SIGNAL IMAGES BY TELECENTRIC EXCITATION AND IMAGE GENERATION OPTICS.
EP1700922B1 (en) 2005-03-10 2016-08-24 Roche Diagnostics GmbH 3-Substituted 5-Nitroindole derivatives and labeled oligonucleotide probes containing them
EP1930730B1 (en) * 2005-03-10 2019-08-14 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes
US7759469B2 (en) 2005-03-10 2010-07-20 Roche Diagnostics Operations, Inc. Labeling reagent
US8615368B2 (en) 2005-03-10 2013-12-24 Gen-Probe Incorporated Method for determining the amount of an analyte in a sample
US7680604B2 (en) 2005-03-11 2010-03-16 Roche Molecular Systems, Inc. PCR elbow determination by rotational transform after zero slope alignment
US7763210B2 (en) 2005-07-05 2010-07-27 3M Innovative Properties Company Compliant microfluidic sample processing disks
US7323660B2 (en) 2005-07-05 2008-01-29 3M Innovative Properties Company Modular sample processing apparatus kits and modules
US7754474B2 (en) 2005-07-05 2010-07-13 3M Innovative Properties Company Sample processing device compression systems and methods
US9017964B2 (en) 2005-08-26 2015-04-28 Denator Aktiebolag Preparing biological samples for analysis
US20070048877A1 (en) * 2005-08-26 2007-03-01 Karl Skold Method and device for preparing a biological sample for biological analyses
US7569367B2 (en) 2005-09-07 2009-08-04 Roche Diagnostics Operations, Inc. Nucleic acid preparation from whole blood for use in diagnosis of transmissible spongiform encephalopathy
US7991558B2 (en) 2005-09-29 2011-08-02 Roche Molecular Systems, Inc. Systems and methods for determining real-time PCR cycle thresholds using cluster analysis
US7991561B2 (en) 2005-09-29 2011-08-02 Roche Molecular Systems, Inc. Ct determination by cluster analysis with variable cluster endpoint
US7781165B2 (en) 2005-10-19 2010-08-24 Roche Diagnostics Operations, Inc. Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification
US7875425B2 (en) 2005-11-09 2011-01-25 Applied Biosystems, Llc Methods for monitoring polymerase chain reactions
GB0522972D0 (en) * 2005-11-11 2005-12-21 Integrated Design Ltd Thermal cycler
DE502005008153D1 (en) 2005-11-23 2009-10-29 Roche Diagnostics Gmbh Polynucleotide with phosphate mimetic
US7680868B2 (en) 2005-12-20 2010-03-16 Roche Molecular Systems, Inc. PCR elbow determination by use of a double sigmoid function curve fit with the Levenburg-Marquardt algorithm and normalization
US7991562B2 (en) 2005-12-20 2011-08-02 Roche Molecular Systems, Inc. PCR elbow determination using curvature analysis of a double sigmoid
US7668663B2 (en) 2005-12-20 2010-02-23 Roche Molecular Systems, Inc. Levenberg-Marquardt outlier spike removal method
US7844403B2 (en) * 2005-12-20 2010-11-30 Roche Molecular Systems, Inc. Temperature step correction with double sigmoid Levenberg-Marquardt and robust linear regression
US7981606B2 (en) 2005-12-21 2011-07-19 Roche Molecular Systems, Inc. Control for nucleic acid testing
JP5022383B2 (en) 2006-02-27 2012-09-12 エフ．ホフマン−ラ ロシュ アーゲー PCR hot start with magnesium blockade
US8293473B2 (en) 2006-04-04 2012-10-23 Labonnet Ltd. Assessment of reaction kinetics compatibility between polymerase chain reactions
US8119352B2 (en) 2006-06-20 2012-02-21 Cepheld Multi-stage amplification reactions by control of sequence replication times
US8097419B2 (en) 2006-09-12 2012-01-17 Longhorn Vaccines & Diagnostics Llc Compositions and method for rapid, real-time detection of influenza A virus (H1N1) swine 2009
US8535888B2 (en) 2006-12-29 2013-09-17 Mayo Foundation For Medical Education And Research Compositions and methods for detecting methicillin-resistant S. aureus
EP1956097A1 (en) 2007-02-06 2008-08-13 bioMerieux B.V. Method for discriminating single nucleotide polymorphisms (SNPs)
WO2008102772A1 (en) * 2007-02-22 2008-08-28 Toyo Boseki Kabushiki Kaisha Nucleic acid amplification system
US9475051B2 (en) 2007-02-27 2016-10-25 Sony Corporation Nucleic acid amplifier
JP4458133B2 (en) * 2007-02-27 2010-04-28 ソニー株式会社 Nucleic acid amplification equipment
JP5191041B2 (en) 2007-04-05 2013-04-24 エフ．ホフマン−ラ ロシュ アーゲー Rapid one-step RT-PCR
WO2008126827A1 (en) 2007-04-06 2008-10-23 Universal Bio Research Co., Ltd. Temperature control device, and temperature control method
JP5444632B2 (en) * 2007-04-26 2014-03-19 東洋紡株式会社 Nucleic acid amplification method and container used therefor
WO2008146754A1 (en) 2007-05-23 2008-12-04 Trust Co., Ltd. Container for liquid reaction mixture, reaction-promoting device using the same and method therefor
US11041215B2 (en) 2007-08-24 2021-06-22 Longhorn Vaccines And Diagnostics, Llc PCR ready compositions and methods for detecting and identifying nucleic acid sequences
US8386184B2 (en) 2007-08-28 2013-02-26 Becton, Dickinson And Company Systems and methods for determining an amount of starting reagent using the polymerase chain reaction
DE102007041864B4 (en) 2007-09-04 2012-05-03 Sirs-Lab Gmbh Method for the detection of bacteria and fungi
US20090119020A1 (en) 2007-09-25 2009-05-07 Roche Molecular Systems, Inc. Pcr elbow determination using quadratic test for curvature analysis of a double sigmoid
EP2209909B1 (en) 2007-09-28 2011-04-13 3M Innovative Properties Company Dual oligonucleotide method of nucleic acid detection
EP3181228B1 (en) 2007-10-02 2020-07-29 Labrador Diagnostics LLC Modular point-of-care devices and uses thereof
JP5178149B2 (en) * 2007-10-31 2013-04-10 キヤノン株式会社 Real-time PCR method and apparatus for target nucleic acid
DE102007063102B4 (en) 2007-12-28 2022-02-10 Robert Bosch Gmbh Method for detecting a periodically pulsating operating parameter
EP2238459B1 (en) 2008-01-23 2019-05-08 Roche Diagnostics GmbH Integrated instrument performing synthesis and amplification
US20090203022A1 (en) * 2008-02-07 2009-08-13 Arizona Board Of Regents For And On Behalf Of Arizona State University Analysis
WO2009100215A2 (en) 2008-02-08 2009-08-13 Mayo Foundation For Medical Education And Research Detection of clostridium difficile
DE102008010436A1 (en) * 2008-02-21 2009-09-03 Berthold Technologies Gmbh & Co. Kg Apparatus and method for measuring luminescence and fluorescence of transfected cells or organ parts
JP2009201444A (en) * 2008-02-29 2009-09-10 Hitachi High-Technologies Corp Nucleic acid analyzer
CA2658520C (en) 2008-03-19 2016-11-08 F. Hoffmann-La Roche Ag Nucleic acid amplification in the presence of modified randomers
GB0806676D0 (en) 2008-04-12 2008-05-14 Environment Agency The Environmental monitoring
ES2439951T3 (en) 2008-06-06 2014-01-27 F. Hoffmann-La Roche Ag Multiplex detection and quantification of internally controlled microbial nucleic acids
EP2138588A1 (en) 2008-06-23 2009-12-30 Koninklijke Philips Electronics N.V. Melting curve measurement during amplification
US8097412B2 (en) 2008-07-12 2012-01-17 Biodiagnostics, Inc. DNA-based test for detection of annual and intermediate ryegrass
EP2148187A1 (en) 2008-07-25 2010-01-27 Roche Diagnostics GmbH Stimulation and optical display system for fluorescence detection
CA2638458A1 (en) 2008-07-31 2010-01-31 Spartan Bioscience Inc. Thermal recycling by positioning relative to fixed-temperature source
US7910720B2 (en) 2008-09-09 2011-03-22 Roche Diagnostics Operations, Inc. Polyanion for improved nucleic acid amplification
US8219324B2 (en) 2008-09-12 2012-07-10 Roche Molecular Systems, Inc. Real-time PCR elbow calling by equation-less algorithm
CA2682439A1 (en) 2008-10-17 2010-04-17 F. Hoffmann-La Roche Ag Cell monitoring and molecular analysis
US8346485B2 (en) 2008-11-25 2013-01-01 Quest Diagnostics Investments Incorporated Methods and apparatuses for estimating initial target nucleic acid concentration in a sample by modeling background signal and cycle-dependent amplification efficiency of a polymerase chain reaction
CA2688174C (en) 2008-12-19 2018-08-07 F. Hoffmann-La Roche Ag Dry composition of reaction compounds with stabilized polymerase
KR101423936B1 (en) * 2009-03-11 2014-07-29 (주)바이오니아 Universal automatic apparatus for real time monitoring of products of nucleic acid amplification reaction and method thereof
US8900854B2 (en) 2009-03-31 2014-12-02 Kanagawa Academy Of Science And Technology Liquid reflux high-speed gene amplification device
EP2443256A1 (en) 2009-06-15 2012-04-25 BG Research Ltd Nucleic acid detection
US8219366B2 (en) 2009-08-26 2012-07-10 Roche Molecular Sytems, Inc. Determination of elbow values for PCR for parabolic shaped curves
US10288632B2 (en) * 2009-09-21 2019-05-14 Pocared Diagnostics Ltd. System for conducting the identification of bacteria in biological samples
FI20096021A0 (en) 2009-10-06 2009-10-06 Wallac Oy Optical measuring instrument
US8834792B2 (en) 2009-11-13 2014-09-16 3M Innovative Properties Company Systems for processing sample processing devices
US20110250598A1 (en) 2010-04-12 2011-10-13 Ulrike Fischer Detergent free polymerases
CN103543282A (en) 2010-07-23 2014-01-29 贝克曼考尔特公司 System for processing samples
CN103270171B (en) 2010-07-29 2014-09-17 霍夫曼-拉罗奇有限公司 Generic pcr
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
CA2802563C (en) 2010-07-29 2018-08-28 F. Hoffmann-La Roche Ag Preparation of nucleic acids from different types of biological fluid samples
CN103069007A (en) 2010-07-29 2013-04-24 霍夫曼-拉罗奇有限公司 Qualitative and quantitative detection of microbial nucleic acids
CA2802567C (en) 2010-07-29 2017-12-05 F. Hoffmann La-Roche Ag Control nucleic acids for multiple parameters
EP2625286B1 (en) 2010-10-04 2020-04-15 Roche Diagniostics GmbH Method for cell lysis in a pcr reaction buffer
WO2012045668A1 (en) 2010-10-04 2012-04-12 Roche Diagnostics Gmbh Method for cell lysis in a rt-pcr reaction buffer
EP2625285B1 (en) 2010-10-04 2020-04-29 Roche Diagniostics GmbH Method for cell lysis and pcr within the same reaction vessel
CN103237880B (en) 2010-10-15 2016-08-03 环球生物研究株式会社 Utilize nucleic acid automatic processing device and the method thereof of multi-functional dispensing unit
US8361720B2 (en) * 2010-11-15 2013-01-29 Exact Sciences Corporation Real time cleavage assay
EP2465945A1 (en) 2010-12-17 2012-06-20 F. Hoffmann-La Roche AG Generic matrix for control nucleic acids
RU2620922C2 (en) 2011-01-21 2017-05-30 Теранос, Инк. Systems and methods for optimization of sample use
WO2012114562A1 (en) 2011-02-22 2012-08-30 ユニバーサル・バイオ・リサーチ株式会社 Reaction container and method for producing same
EP2678664B1 (en) 2011-02-24 2019-08-07 Gen-Probe Incorporated Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector
KR20140022399A (en) 2011-05-18 2014-02-24 쓰리엠 이노베이티브 프로퍼티즈 컴파니 Systems and methods for volumetric metering on a sample processing device
EP2709760B1 (en) 2011-05-18 2019-06-05 DiaSorin S.p.A. Systems and methods for valving on a sample processing device
JP6235462B2 (en) 2011-05-18 2017-11-22 スリーエム イノベイティブ プロパティズ カンパニー System and method for detecting the presence of a selected volume of material in a sample processing apparatus
US9034581B2 (en) 2011-05-26 2015-05-19 Roche Molecular Systems, Inc. Compositions and methods for detection of Staphylococcus aureus
US8475739B2 (en) 2011-09-25 2013-07-02 Theranos, Inc. Systems and methods for fluid handling
US9664702B2 (en) 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
US20140170735A1 (en) 2011-09-25 2014-06-19 Elizabeth A. Holmes Systems and methods for multi-analysis
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
US10012664B2 (en) 2011-09-25 2018-07-03 Theranos Ip Company, Llc Systems and methods for fluid and component handling
US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
SG10201602113TA (en) 2011-09-30 2016-04-28 Life Technologies Corp Systems And Methods For Biological Analysis
US8973736B2 (en) 2011-11-07 2015-03-10 Beckman Coulter, Inc. Magnetic damping for specimen transport system
BR112014011035A2 (en) 2011-11-07 2017-06-13 Beckman Coulter, Inc. aliquot system and workflow
BR112014010955A2 (en) 2011-11-07 2017-06-06 Beckman Coulter Inc system and method for processing samples
ES2729283T3 (en) 2011-11-07 2019-10-31 Beckman Coulter Inc Centrifugal and workflow system
ES2844324T3 (en) 2011-11-07 2021-07-21 Beckman Coulter Inc Robotic arm
KR20140091033A (en) 2011-11-07 2014-07-18 베크만 컬터, 인코포레이티드 Specimen container detection
WO2013139860A1 (en) 2012-03-21 2013-09-26 Roche Diagnostics Gmbh Methods for assessing rna quality
US9982313B2 (en) 2012-08-17 2018-05-29 Roche Molecular Systems, Inc. Compositions and methods for detection of herpes simplex virus 1 and 2
DE102012108158B4 (en) 2012-09-03 2016-03-17 Johann Wolfgang Goethe-Universität Capillary cell, assembly and method for receiving, positioning and examining a microscopic sample
US10176293B2 (en) 2012-10-02 2019-01-08 Roche Molecular Systems, Inc. Universal method to determine real-time PCR cycle threshold values
EP2722399A1 (en) 2012-10-18 2014-04-23 Roche Diagniostics GmbH Method for preventing high molecular weight products during amplification
EP2945742B1 (en) * 2013-01-18 2024-07-10 Biomeme, Inc. Analytic device
WO2014122924A1 (en) 2013-02-05 2014-08-14 パナソニックヘルスケア株式会社 Analysis device, genetic analysis method, analysis receptacle, and control method for fuzzy control
AU2013202788B2 (en) * 2013-03-14 2015-10-01 Gen-Probe Incorporated Indexing signal detection module
US10626440B2 (en) 2013-05-21 2020-04-21 Universal Bio Research Co., Ltd. Sequencer pretreatment device and method thereof
US10072288B2 (en) 2013-11-11 2018-09-11 Roche Molecular Systems, Inc. Detecting single nucleotide polymorphism using overlapped primer and melting probe
US9745618B2 (en) 2014-11-19 2017-08-29 Roche Molecular Systems, Inc. Photoblocked probes and methods for sequential detection of nucleic acids
US9920381B2 (en) 2014-12-02 2018-03-20 Roche Molecular Systems, Inc. Compositions and methods for detecting MECC-containing methicillin-resistant Staphylococcus aureus
FR3035411A1 (en) * 2015-04-23 2016-10-28 Morpho METHOD FOR AMPLIFYING NUCLEIC ACID FOR ANALYSIS, CORRESPONDING AMPLIFICATION MACHINE, AND CARTRIDGE FOR THE MACHINE
EP3355914B1 (en) 2015-09-29 2024-03-06 The General Hospital Corporation A composition comprising bcg for reducing cholesterol.
US10391498B2 (en) 2015-12-11 2019-08-27 Spartan Bioscience Inc. Systems and methods for nucleic acid amplification
US10655190B2 (en) 2016-03-11 2020-05-19 Roche Molecular Systems, Inc. Compositions and methods for detection of Zika virus
US20190176153A1 (en) 2016-05-18 2019-06-13 Roche Sequencing Solutions, Inc. Quantitative real time pcr amplification using an electrowetting-based device
CA3025585C (en) 2016-05-27 2022-07-19 F. Hoffmann-La Roche Ag Compositions and methods for detection of trichomonas vaginalis
US10612101B2 (en) 2016-05-27 2020-04-07 Roche Molecular Systems, Inc. Compositions and methods for detection of Mycoplasma genitalium
US20170356025A1 (en) 2016-06-14 2017-12-14 Roche Molecular Systems, Inc. Internal control probes for improving pcr assay performance
CA3031994A1 (en) 2016-07-29 2018-02-01 Juno Therapeutics, Inc. Methods for assessing the presence or absence of replication competent virus
CN109642253A (en) 2016-08-02 2019-04-16 豪夫迈·罗氏有限公司 For improving the auxiliary oligonucleotide of the amplification of nucleic acid and the efficiency of detected/quantified
CN107964507B (en) * 2016-10-18 2022-09-27 财团法人工业技术研究院 Thermal convection polymerase chain reaction device and optical detection method thereof
US10793923B2 (en) 2016-11-09 2020-10-06 Roche Molecular Systems, Inc. Compositions and methods for detection of BK virus
US20190211377A1 (en) 2016-12-22 2019-07-11 Roche Molecular Systems, Inc. Cobra probes to detect a marker for epidemic ribotypes of clostridium difficile
US10793901B2 (en) 2016-12-28 2020-10-06 Roche Molecular Systems, Inc. Reversibly protected nucleotide reagents with high thermal stability
JP7044715B2 (en) 2016-12-28 2022-03-30 株式会社Mirai Genomics Analysis equipment
US20180363044A1 (en) 2017-06-14 2018-12-20 Roche Molecular Systems, Inc. Compositions and methods for improving the thermal stability of nucleic acid amplification reagents
US10760137B2 (en) 2017-07-18 2020-09-01 Roche Molecular Systems, Inc. Compositions and methods for detection of Babesia
CN111356768A (en) 2017-09-15 2020-06-30 生米公司 Method and system for automated sample processing
WO2019063661A1 (en) 2017-09-29 2019-04-04 Roche Diagnostics Gmbh Compositions and methods for detection of trichomonas vaginalis
US11078534B2 (en) 2017-11-27 2021-08-03 Roche Molecular Systems, Inc. Photocleavable nucleotide reagents with high stability
EP3746569A1 (en) 2018-01-31 2020-12-09 Juno Therapeutics, Inc. Methods and reagents for assessing the presence or absence of replication competent virus
US20200398274A1 (en) * 2018-02-07 2020-12-24 Jaydeep BHATTACHARYA A low cost microfluidic device for dna/rna isolation, purification and amplification using chip based pcr/ rt- pcr for biosensing applications
US20190292596A1 (en) 2018-03-21 2019-09-26 Roche Molecular Systems, Inc. Modified nucleoside phosphates with high thermal stability
US11384376B2 (en) 2018-05-31 2022-07-12 Roche Molecular Systems, Inc. Reagents and methods for post-synthetic modification of nucleic acids
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
WO2020032226A1 (en) 2018-08-10 2020-02-13 株式会社Ｍｉｒａｉ Ｇｅｎｏｍｉｃｓ Analysis device
CN113166802A (en) 2018-12-03 2021-07-23 豪夫迈·罗氏有限公司 Compositions and methods for detecting candida auriculae
WO2020191193A1 (en) 2019-03-21 2020-09-24 Biomeme, Inc. Multi-function analytic devices
CN113874115A (en) * 2019-05-01 2021-12-31 卢米耐克斯公司 Apparatus and method for thermal cycling of samples
CN113785073A (en) 2019-05-02 2021-12-10 豪夫迈·罗氏有限公司 Preferential/selective amplification of RNA versus DNA targets using dITP based on strand isolation temperature
WO2020225231A1 (en) 2019-05-07 2020-11-12 F. Hoffmann-La Roche Ag Compositions and methods for detection of neisseria gonorroheae
EP4004240B1 (en) 2019-07-25 2024-03-13 F. Hoffmann-La Roche AG Compositions and methods for detection of epstein barr virus (ebv)
EP4022086A1 (en) 2019-08-27 2022-07-06 F. Hoffmann-La Roche AG Compositions and methods for amplification and detection of hepatitis b virus rna, including hbv rna transcribed from cccdna
US11441167B2 (en) 2019-11-20 2022-09-13 Roche Molecular Systems, Inc. Compositions and methods for rapid identification and phenotypic antimicrobial susceptibility testing of bacteria and fungi
WO2021116037A1 (en) 2019-12-09 2021-06-17 F. Hoffmann-La Roche Ag Dicationic fluorescent dyes
CN114867871A (en) 2019-12-27 2022-08-05 豪夫迈·罗氏有限公司 Compositions and methods for detecting methicillin-resistant staphylococcus aureus
CN116171333A (en) 2020-03-09 2023-05-26 豪夫迈·罗氏有限公司 Compositions and methods for detecting severe acute respiratory syndrome coronavirus 2 (SARS-COV-2), influenza A and influenza B
CN116457099A (en) 2020-09-18 2023-07-18 生米公司 Portable device and method for analyzing a sample
US20240102110A1 (en) 2020-12-04 2024-03-28 Roche Molecular Systems, Inc. Compositions and methods for detection of malaria
JP7209980B2 (en) 2020-12-11 2023-01-23 東洋紡株式会社 An antibody that specifically binds to the 5' to 3' exonuclease active domain of a DNA polymerase
JP2022099005A (en) 2020-12-22 2022-07-04 船井電機株式会社 Micro fluid device and nucleic acid amplification method
US20220205020A1 (en) 2020-12-30 2022-06-30 Roche Molecular Systems, Inc. Compositions and methods for detection of bacteria and fungi associated with bacterial and candida vaginosis
JP2024505686A (en) 2021-02-05 2024-02-07 エフ． ホフマン－ラ ロシュ アーゲー Compositions and methods for the detection of human parainfluenza virus 1-4 (HPIV1-4)
US20220290221A1 (en) 2021-03-15 2022-09-15 Roche Molecular Systems, Inc. Compositions and methods for detecting severe acute respiratory syndrome coronavirus 2 (sars-cov-2) variants having spike protein mutations
US20240165628A1 (en) 2021-03-19 2024-05-23 Bg Research Ltd An apparatus and associated methods for thermal cycling
CN117858960A (en) 2021-05-06 2024-04-09 豪夫迈·罗氏有限公司 Compositions and methods for detecting hepatitis delta virus by dual target assays
CN113355230B (en) * 2021-07-06 2023-11-24 宁波胤瑞生物医学仪器有限责任公司 Chip locking device
TW202415452A (en) * 2021-07-08 2024-04-16 安盛生科股份有限公司 System and method to perform quantitative polymerase chain reaction
MX2024003654A (en) 2021-09-23 2024-06-19 N6 Tec Inc Methods and systems for sample analysis.
WO2023079032A1 (en) 2021-11-05 2023-05-11 F. Hoffmann-La Roche Ag Compositions and methods for detection of malaria
WO2023089186A1 (en) 2021-11-22 2023-05-25 F. Hoffmann-La Roche Ag Compositions and methods for detecting vana and/or vanb genes associated with multidrug resistance
EP4441252A1 (en) 2021-12-03 2024-10-09 Medicinal Genomics Corporation Psilocybe assay
WO2024003260A1 (en) 2022-06-30 2024-01-04 F. Hoffmann-La Roche Ag Compositions and methods for detecting lymphogranuloma venereum (lgv) serovars of chlamydia trachomatis
WO2024042042A1 (en) 2022-08-24 2024-02-29 F. Hoffmann-La Roche Ag Compositions and methods for detecting monkeypox virus
WO2024059493A1 (en) 2022-09-13 2024-03-21 Medicinal Genomics Corporation Psilocybe assay
CN115555060A (en) * 2022-09-22 2023-01-03 冰山松洋生物科技(大连)有限公司 Environmental test chamber
WO2024141442A1 (en) 2022-12-25 2024-07-04 F. Hoffmann-La Roche Ag Compositions and methods for detecting carbapenem resistant acinetobacter calcoaceticus-baumannii (crab)
WO2024175749A1 (en) 2023-02-25 2024-08-29 F. Hoffmann-La Roche Ag Compositions and methods for candida species detection
WO2024184165A1 (en) 2023-03-03 2024-09-12 F. Hoffmann-La Roche Ag Compositions and methods for the detection of group b streptococcus ( streptococcus agalactiae) and clindamycin resistance gene determinants

Citations (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3219416A (en) * 1962-10-30 1965-11-23 Scientific Industries Apparatus for the automatic chemical sequential treatment and analysis of small quantities of material
US3556659A (en) * 1966-02-03 1971-01-19 Applied Physics Corp Laser-excited raman spectrometer
FR2122187A5 (en) * 1971-01-12 1972-08-25 Damon Corp
US3999868A (en) * 1974-11-29 1976-12-28 Manuel Sanz Unit for use in optical analysis
AU528259B2 (en) * 1978-09-14 1983-04-21 Union Carbide Corp Method and apparatus for assaying liquid materials
EP0171140A2 (en) * 1984-05-15 1986-02-12 The University of Tokyo Automatic cycling reaction apparatus and automatic analyzing apparatus using the same
EP0211334A1 (en) * 1985-08-05 1987-02-25 Martin Marietta Energy Systems, Inc. Method and apparatus for automated processing and aliquoting of whole blood samples for analysis in a centrifugal fast analyzer
EP0229943A2 (en) * 1985-12-23 1987-07-29 Molecular Biosystems, Inc. Fluorescent stokes shift probes for polynucleotide hybridization assays
EP0318255A2 (en) * 1987-11-23 1989-05-31 EASTMAN KODAK COMPANY (a New Jersey corporation) Cuvette
US4908112A (en) * 1988-06-16 1990-03-13 E. I. Du Pont De Nemours & Co. Silicon semiconductor wafer for analyzing micronic biological samples
EP0404258A2 (en) * 1989-06-20 1990-12-27 Claudio Bonini Test-tube with lenticular outside surface particularly for automatized clinical analysis
WO1992020778A1 (en) * 1991-05-24 1992-11-26 Kindconi Pty Limited Biochemical reaction control
EP0519623A2 (en) * 1991-06-07 1992-12-23 Ciba Corning Diagnostics Corp. Multiple surface evanescent wave sensor system
WO1993020240A1 (en) * 1992-04-06 1993-10-14 Abbott Laboratories Method and device for detection of nucleic acid or analyte using total internal reflectance
US5268486A (en) * 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
EP0580362A1 (en) * 1992-07-17 1994-01-26 Tosoh Corporation Fluorescence detecting apparatus
EP0636413A2 (en) * 1993-07-28 1995-02-01 The Perkin-Elmer Corporation Nucleic acid amplification reaction apparatus and method
JPH0731500A (en) * 1993-07-15 1995-02-03 Hamamatsu Photonics Kk Detector for denaturation of nucleic acids
EP0640828A1 (en) * 1993-08-27 1995-03-01 F. Hoffmann-La Roche AG Monitoring multiple reactions simultaneously and analyzing same
WO1995021266A1 (en) * 1994-02-01 1995-08-10 The Regents Of The University Of California Probes labelled with energy transfer coupled dyes
WO1995021382A2 (en) * 1994-02-01 1995-08-10 Fields Robert E Molecular analyzer and method of use
EP0674009A2 (en) * 1994-03-14 1995-09-27 Becton, Dickinson and Company Nucleic acid amplification method and apparatus
US5455175A (en) * 1990-06-04 1995-10-03 University Of Utah Research Foundation Rapid thermal cycling device
WO1996006354A1 (en) * 1994-08-24 1996-02-29 Biocircuits Corporation Device for use in analyte detection assays
EP0711840A2 (en) * 1994-11-09 1996-05-15 Hitachi, Ltd. DNA analyzing method and device therefor
EP0805190A2 (en) * 1996-05-03 1997-11-05 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3616264A (en) 1969-06-30 1971-10-26 Beckman Instruments Inc Temperature-controlled discrete sample analyzer
IT1101295B (en) 1978-11-22 1985-09-28 Erba Strumentazione GAS-CHROMATOGRAPHIC CHAMBER
US4420679A (en) 1982-02-26 1983-12-13 Delta Associates, Inc. Gas chromatographic oven using symmetrical flow of preheated - premixed ambient air
US4762413A (en) * 1984-09-07 1988-08-09 Olympus Optical Co., Ltd. Method and apparatus for measuring immunological reaction with the aid of fluctuation in intensity of scattered light
US5449621A (en) * 1989-07-31 1995-09-12 Biotope, Inc. Method for measuring specific binding assays
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
DE4234086A1 (en) † 1992-02-05 1993-08-12 Diagen Inst Molekularbio METHOD FOR DETERMINING NUCLEIC ACID SEQUENCES AMPLIFIED IN VITRO
ES2082256T3 (en) * 1992-03-23 1996-03-16 Hoffmann La Roche DNA DETECTION METHOD.
WO1994021780A1 (en) * 1993-03-18 1994-09-29 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture Apparatus for the quantitative determination of particulate analytes
EP0700514B1 (en) * 1993-05-18 2001-11-28 University of Utah Research Foundation Apparatus and methods for multianalyte homogeneous fluoroimmunoassays
EP1921169B1 (en) * 1993-11-12 2012-02-15 PHRI Properties, Inc. Hybridization probes for nucleic acid detection, universal stems, methods and kits
EP0706649B1 (en) * 1994-04-29 2001-01-03 Perkin-Elmer Corporation Method and apparatus for real time detection of nucleic acid amplification products
JPH10500572A (en) * 1994-05-23 1998-01-20 バイオトロニクス コーポレーション Method for detecting target nucleic acid
US5622821A (en) * 1994-06-29 1997-04-22 The Regents Of The University Of California Luminescent lanthanide chelates and methods of use

Patent Citations (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3219416A (en) * 1962-10-30 1965-11-23 Scientific Industries Apparatus for the automatic chemical sequential treatment and analysis of small quantities of material
US3556659A (en) * 1966-02-03 1971-01-19 Applied Physics Corp Laser-excited raman spectrometer
FR2122187A5 (en) * 1971-01-12 1972-08-25 Damon Corp
US3999868A (en) * 1974-11-29 1976-12-28 Manuel Sanz Unit for use in optical analysis
AU528259B2 (en) * 1978-09-14 1983-04-21 Union Carbide Corp Method and apparatus for assaying liquid materials
EP0171140A2 (en) * 1984-05-15 1986-02-12 The University of Tokyo Automatic cycling reaction apparatus and automatic analyzing apparatus using the same
EP0211334A1 (en) * 1985-08-05 1987-02-25 Martin Marietta Energy Systems, Inc. Method and apparatus for automated processing and aliquoting of whole blood samples for analysis in a centrifugal fast analyzer
EP0229943A2 (en) * 1985-12-23 1987-07-29 Molecular Biosystems, Inc. Fluorescent stokes shift probes for polynucleotide hybridization assays
US5268486A (en) * 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
EP0318255A2 (en) * 1987-11-23 1989-05-31 EASTMAN KODAK COMPANY (a New Jersey corporation) Cuvette
US4908112A (en) * 1988-06-16 1990-03-13 E. I. Du Pont De Nemours & Co. Silicon semiconductor wafer for analyzing micronic biological samples
EP0404258A2 (en) * 1989-06-20 1990-12-27 Claudio Bonini Test-tube with lenticular outside surface particularly for automatized clinical analysis
US5455175A (en) * 1990-06-04 1995-10-03 University Of Utah Research Foundation Rapid thermal cycling device
WO1992020778A1 (en) * 1991-05-24 1992-11-26 Kindconi Pty Limited Biochemical reaction control
EP0519623A2 (en) * 1991-06-07 1992-12-23 Ciba Corning Diagnostics Corp. Multiple surface evanescent wave sensor system
WO1993020240A1 (en) * 1992-04-06 1993-10-14 Abbott Laboratories Method and device for detection of nucleic acid or analyte using total internal reflectance
EP0580362A1 (en) * 1992-07-17 1994-01-26 Tosoh Corporation Fluorescence detecting apparatus
JPH0731500A (en) * 1993-07-15 1995-02-03 Hamamatsu Photonics Kk Detector for denaturation of nucleic acids
US5599504A (en) * 1993-07-15 1997-02-04 Hamamatsu Photonics K.K. Apparatus for detecting denaturation of nucleic acid
EP0636413A2 (en) * 1993-07-28 1995-02-01 The Perkin-Elmer Corporation Nucleic acid amplification reaction apparatus and method
EP0640828A1 (en) * 1993-08-27 1995-03-01 F. Hoffmann-La Roche AG Monitoring multiple reactions simultaneously and analyzing same
WO1995021266A1 (en) * 1994-02-01 1995-08-10 The Regents Of The University Of California Probes labelled with energy transfer coupled dyes
WO1995021382A2 (en) * 1994-02-01 1995-08-10 Fields Robert E Molecular analyzer and method of use
EP0674009A2 (en) * 1994-03-14 1995-09-27 Becton, Dickinson and Company Nucleic acid amplification method and apparatus
WO1996006354A1 (en) * 1994-08-24 1996-02-29 Biocircuits Corporation Device for use in analyte detection assays
EP0711840A2 (en) * 1994-11-09 1996-05-15 Hitachi, Ltd. DNA analyzing method and device therefor
EP0805190A2 (en) * 1996-05-03 1997-11-05 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
MUJUMDAR R B ET AL: "CYANINE DYE LABELING REAGENTS CONTAINING ISOTHIOCYANATE GROUPS", CYTOMETRY, vol. 10, 1 January 1989 (1989-01-01), pages 11 - 19, XP000575307 *
MULLIS ET AL.: "the polymerase chain reaction", 1994, BIRKHAUSER, BOSTON (US), XP002042556 *
TYAGI S ET AL: "MOLECULAR BEACONS: PROBES THAT FLUORESCE UPON HYBRIDIZATION", BIO/TECHNOLOGY, vol. 14, 1 March 1996 (1996-03-01), pages 303 - 308, XP000196024 *
WEAST: "handbook of chemistry and physics", 1972, CRC, CLEVELAND (USA), XP002042557 *
WITTWER C T ET AL: "RAPID CYCLE ALLELE-SPECIFIC AMPLIFICATION: STUDIES WITH THE CYSTIC FIBROSIS AF508 LOCUS", CLINICAL CHEMISTRY, vol. 39, no. 5, 1993, pages 804 - 809, XP000560381 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9481912B2 (en) 2006-09-12 2016-11-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and identifying nucleic acid sequences in biological samples
US9416416B2 (en) 2007-10-01 2016-08-16 Longhorn Vaccines And Diagnostics, Llc Biological specimen collection/transport compositions and methods
US8623602B2 (en) 2008-08-01 2014-01-07 Roche Diagnostics Operations, Inc. Lysis and reverse transcription for MRNA quantification

Also Published As

Publication number Publication date
AU3154797A (en) 1998-01-05
DE69734587T2 (en) 2006-07-20
EP1442794A3 (en) 2005-05-11
AU727296B2 (en) 2000-12-07
NZ333137A (en) 2000-03-27
ATE309388T1 (en) 2005-11-15
EP2298931A1 (en) 2011-03-23
JP2000512138A (en) 2000-09-19
ATE321891T1 (en) 2006-04-15
NZ333135A (en) 2000-02-28
EP1674585A1 (en) 2006-06-28
DE69735563T2 (en) 2007-05-16
CA2256612A1 (en) 1997-12-11
WO1997046707A2 (en) 1997-12-11
DE69735563D1 (en) 2006-05-18
WO1997046707A3 (en) 1998-10-08
EP2298931B2 (en) 2020-06-24
WO1997046712A2 (en) 1997-12-11
JP2000511435A (en) 2000-09-05
EP0906449A2 (en) 1999-04-07
DE69740030D1 (en) 2010-12-02
DE69727932D1 (en) 2004-04-08
EP1493826A1 (en) 2005-01-05
EP2298931A9 (en) 2011-06-01
EP2298931B1 (en) 2013-08-07
DE69738605D1 (en) 2008-05-08
CA2256773A1 (en) 1997-12-11
ATE260988T1 (en) 2004-03-15
EP0912760A2 (en) 1999-05-06
EP1493826B1 (en) 2008-03-26
DE69738605T2 (en) 2009-04-30
EP1704922A3 (en) 2007-12-12
ES2260562T3 (en) 2006-11-01
JP4281877B2 (en) 2009-06-17
ATE485397T1 (en) 2010-11-15
DK2298931T3 (en) 2013-11-11
JP4278712B2 (en) 2009-06-17
CA2256612C (en) 2012-09-25
EP1442794B1 (en) 2006-03-29
ES2304573T3 (en) 2008-10-16
ES2253778T3 (en) 2006-06-01
ES2215230T3 (en) 2004-10-01
ATE390491T1 (en) 2008-04-15
EP1674585B1 (en) 2010-10-20
DE69734587D1 (en) 2005-12-15
CA2256773C (en) 2010-01-26
ES2434258T3 (en) 2013-12-16
DE69727932T2 (en) 2005-03-10
AU729644B2 (en) 2001-02-08
PT2298931E (en) 2013-11-11
EP1704922A2 (en) 2006-09-27
EP0906449B1 (en) 2004-03-03
EP1442794A2 (en) 2004-08-04
EP0912760B1 (en) 2005-11-09
AU3380097A (en) 1998-01-05
ES2354902T3 (en) 2011-03-21

Similar Documents

Publication Publication Date Title
WO1997046712A3 (en) System and method for carrying out and monitoring biological processes
RU2385940C1 (en) Method for real-time detection of nucleic acids by polymerase chain reaction and device for implementation thereof
US5897842A (en) Method and apparatus for thermal cycling and for automated sample preparation with thermal cycling
EP1871903B1 (en) Devices and methods for identifying genomic dna of organisms
US20080176232A1 (en) Reaction System for Performing in the Amplification of Nucleic Acids
ATE233600T1 (en) DEVICE AND VESSELS FOR CARRYING OUT A POLYMERASE CHAIN REACTION
ATE483790T1 (en) SAMPLE BLOCK DEVICE AND METHOD FOR MAINTAINING A MICRO CARD ON A SAMPLE BLOCK
ATE505560T1 (en) REACTION VESSEL FOR REAL-TIME PCR COMPRISING OLIGONUCLEOTIDE SAMPLES WHERE THE AMPLIFICATION PRODUCTS ARE MEASURED BY HYBRIDIZATION AND WITHOUT OPENING THE REACTION VESSEL
WO2008005323A2 (en) Combined thermal devices for thermal cycling
DE69429038D1 (en) Device and method for nucleic acid amplification
RU2008148143A (en) MICROELECTRONIC SENSOR DEVICE FOR DNA DETECTION
WO2007044917A3 (en) Polynucleotide sample preparation device
EP1375673A4 (en) Gene analysis method and analyzer therefor
KR100879496B1 (en) Nucleic acid preparation
AU2001289754A1 (en) Method and device for analysing chemical or biological samples
EP0572023B1 (en) Electrophoretic apparatus
WO2000066995A3 (en) Device for rapid dna sample processing with integrated liquid handling, thermocycling, and purification
JP2004242607A (en) Reactor
US11345097B2 (en) Method of making a frangible seal in a sample processing device
EP3631007B1 (en) Multiplex nucleic acid amplification assay
Arroyo et al. Flow through pcr module of biobriefcase

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH HU IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK TJ TM TR TT UA UG US UZ VN YU AM AZ BY KG KZ MD RU TJ TM

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH KE LS MW SD SZ UG AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH HU IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK TJ TM TR TT UA UG US UZ VN YU AM AZ BY KG KZ MD RU TJ TM

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH KE LS MW SD SZ UG AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF

WWE Wipo information: entry into national phase

Ref document number: 1997929833

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 333137

Country of ref document: NZ

ENP Entry into the national phase

Ref document number: 2256773

Country of ref document: CA

Ref country code: CA

Ref document number: 2256773

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 1019980709899

Country of ref document: KR

WWP Wipo information: published in national office

Ref document number: 1997929833

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWP Wipo information: published in national office

Ref document number: 1019980709899

Country of ref document: KR

WWG Wipo information: grant in national office

Ref document number: 1997929833

Country of ref document: EP

WWG Wipo information: grant in national office

Ref document number: 1019980709899

Country of ref document: KR