WO1997035472B1 - Use of arabinogalactan in cell cryopreservation media - Google Patents
Use of arabinogalactan in cell cryopreservation mediaInfo
- Publication number
- WO1997035472B1 WO1997035472B1 PCT/US1997/004764 US9704764W WO9735472B1 WO 1997035472 B1 WO1997035472 B1 WO 1997035472B1 US 9704764 W US9704764 W US 9704764W WO 9735472 B1 WO9735472 B1 WO 9735472B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- medium
- cryoprotective
- arabinogalactan
- cells
- concentration
- Prior art date
Links
- 229920000189 Arabinogalactan Polymers 0.000 title claims abstract 13
- 239000001904 Arabinogalactan Substances 0.000 title claims abstract 13
- 235000019312 arabinogalactan Nutrition 0.000 title claims abstract 13
- 239000012595 freezing medium Substances 0.000 title abstract 3
- 210000004027 cells Anatomy 0.000 claims abstract 10
- 210000001082 somatic cell Anatomy 0.000 claims abstract 8
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims abstract 6
- 238000007710 freezing Methods 0.000 claims abstract 4
- 239000003795 chemical substances by application Substances 0.000 claims abstract 3
- 210000004962 mammalian cells Anatomy 0.000 claims abstract 3
- 239000000203 mixture Substances 0.000 claims abstract 3
- 238000010257 thawing Methods 0.000 claims abstract 3
- 230000035899 viability Effects 0.000 claims abstract 3
- 241000283690 Bos taurus Species 0.000 claims abstract 2
- 241000282465 Canis Species 0.000 claims abstract 2
- 241000283073 Equus caballus Species 0.000 claims abstract 2
- 241000282414 Homo sapiens Species 0.000 claims abstract 2
- 241000283984 Rodentia Species 0.000 claims abstract 2
- 230000000959 cryoprotective Effects 0.000 claims 19
- 239000002577 cryoprotective agent Substances 0.000 claims 2
- 102000009027 Albumins Human genes 0.000 claims 1
- 108010088751 Albumins Proteins 0.000 claims 1
- 229940064005 Antibiotic throat preparations Drugs 0.000 claims 1
- 229940083879 Antibiotics FOR TREATMENT OF HEMORRHOIDS AND ANAL FISSURES FOR TOPICAL USE Drugs 0.000 claims 1
- 229940042052 Antibiotics for systemic use Drugs 0.000 claims 1
- 229940042786 Antitubercular Antibiotics Drugs 0.000 claims 1
- 210000004369 Blood Anatomy 0.000 claims 1
- 210000000988 Bone and Bones Anatomy 0.000 claims 1
- 210000004556 Brain Anatomy 0.000 claims 1
- 210000000845 Cartilage Anatomy 0.000 claims 1
- 210000002808 Connective Tissue Anatomy 0.000 claims 1
- 102000004127 Cytokines Human genes 0.000 claims 1
- 108090000695 Cytokines Proteins 0.000 claims 1
- 229940093922 Gynecological Antibiotics Drugs 0.000 claims 1
- 229940088597 Hormone Drugs 0.000 claims 1
- 210000003734 Kidney Anatomy 0.000 claims 1
- 210000004185 Liver Anatomy 0.000 claims 1
- 210000004072 Lung Anatomy 0.000 claims 1
- 210000003205 Muscles Anatomy 0.000 claims 1
- 210000002966 Serum Anatomy 0.000 claims 1
- 210000004927 Skin cells Anatomy 0.000 claims 1
- 229940024982 Topical Antifungal Antibiotics Drugs 0.000 claims 1
- 150000001413 amino acids Chemical class 0.000 claims 1
- 210000001776 amniocyte Anatomy 0.000 claims 1
- 239000003242 anti bacterial agent Substances 0.000 claims 1
- 230000001857 anti-mycotic Effects 0.000 claims 1
- 239000002543 antimycotic Substances 0.000 claims 1
- 230000003115 biocidal Effects 0.000 claims 1
- 239000008280 blood Substances 0.000 claims 1
- 230000030833 cell death Effects 0.000 claims 1
- 239000012091 fetal bovine serum Substances 0.000 claims 1
- 239000003102 growth factor Substances 0.000 claims 1
- 239000005556 hormone Substances 0.000 claims 1
- 229940079866 intestinal antibiotics Drugs 0.000 claims 1
- 150000002632 lipids Chemical class 0.000 claims 1
- 229940005935 ophthalmologic Antibiotics Drugs 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 239000012266 salt solution Substances 0.000 claims 1
- 239000003270 steroid hormone Substances 0.000 claims 1
- 210000004881 tumor cells Anatomy 0.000 claims 1
- 239000002609 media Substances 0.000 abstract 4
- 238000003860 storage Methods 0.000 abstract 3
- 238000005138 cryopreservation Methods 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 1
Abstract
Methods and compositions for cryopreserving somatic cells are provided. In one embodiment, a cell cryopreservation medium is provided which includes an effective amount of arabinogalactan to maintain the viability of cells upon freezing, storage and thawing. The cells may be cooled or frozen during storage to a temperature about or below 4 °C, for example, to about - 196 °C. In one preferred embodiment, ultrarefined arabinogalactan is provided in the cryopreservation medium, optionally in combination with a second cryopreservation agent, such as dimethyl sulfoxide. The medium can be used for the cryopreservation of a wide variety of different cell types from different sources. For example, mammalian cells, including porcine, canine, human, equine, rodent and bovine cells can be cryopreserved in the medium. The presence of arabinogalactan in the medium protects the viability of cells in the medium during the process of freezing, storage and thawing.
Claims
AMENDED CLAIMS
[received by the International Bureau on 16 September 1997 ( 16.09.97); original claims 24, 30, 35 and 36 amended; remaining claims unchanged (2 pages)]
21. The method of claim 20 wherein the mammalian cells are selected from the group consisting of porcine, canine, human, rodent, equine and bovine cells.
22. The method of claim 6 wherein the somatic cells are tumor cells.
23. The method of claim 6 wherein the somatic cells are genetically altered mammalian cells.
24. A medium for cryopreserving and cryoprotecting somatic cells comprising arabinogalactan as a cryopreservaiive agent, wherein the medium comprises an effective concentration of cryoprescrvative agent to maintain the viability, and to reduce cell damage and cell death, of cells disposed therein upon freezing and thawing.
25. The cryoprotective medium of claim 24, wherein the medium is frozen to a temperature suitable for cell freezing.
26. The cryoprotective medium of claim 25 wherein the temperature is about or below 4βC.
27. The cryoprotective medium of claim 25 wherein the medium is frozen to a temperature between about -70°C and -200°C.
28. The cryoprotective medium of claim 24 wherein the arabinogalactan is ultrarefined arabinogalactan.
29. The cryoprotective medium of claim 28 wherein the concentration of arabinogalactan in the medium is the maximum concentration soluble in the medium.
30. The cryoprotective medium of claim 28 wherein the concentration of arabinogalactan in the medium is between 10 and 70% (weight/ volume).
31. The cryoprotective medium of claim 30 wherein the concentration of arabinogalactan is between about 20 and 50% (weight/volume) .
32. The cryoprotective medium of claim 31 wherein the concentration of arabinogalactan is between about 44 and 50% (weight/ volume).
33. The cryoprotective medium of claim 28 wherein the medium comprises arabinogalactan in an aqueous buffered isotonic salt solution.
34. The cryoprotective medium of claim 33 wherein the medium further comprises a composition selected from the group consisting of amino acids, cytokines, lipids, growth factors, antibiotics, antimycotics, steroid hormones, protein hormones and albumins.
35. The cryoprotective medium of claim 24 wherein the concentration of arabinogalactan is between 5 and 70% (weight/ volume), further comprising a second cryoprotective agent.
36. The cryoprotective medium oi" claim 35 wherein the second cryoprotective agent is dimethyl sulfoxide ("DMSO").
37. The cryoprotective medium of claim 36 wherein the medium comprises DMSO at a concentration of between about 1 and 10% (volume percent).
38. The cryoprotective medium of claim 36 wherein the medium further comprises serum.
39. The cryoprotective medium of claim 38 wherein the medium comprises fetal bovine serum at a concentration of between about 10 and 40% (volume percent).
40. The cryoprotective medium of claim 24 further comprising somatic cells.
41. The cryoprotective medium of claim 40 wherein the somatic cells are selected from the group consisting of epithelial, connective tissue, muscle, amniocyte nerve, brain, mucosal, blood, cartilage, mammaiy, kidney, liver, pancreatic, bone, corneal, arterial, lung, and skin cells.
42. The cryoprotective medium of claim 40 wherein the somatic cells are derived from the circulatory system.
43. The cryoprotective medium of claim 40 wherein the somatic cells axe mammalian.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP97916960A EP0891134B1 (en) | 1996-03-25 | 1997-03-25 | Use of arabinogalactan in cell cryopreservation media |
| JP53454397A JP4031044B2 (en) | 1996-03-25 | 1997-03-25 | Use of arabinogalactan in cell cryopreservation medium |
| DE69713312T DE69713312T2 (en) | 1996-03-25 | 1997-03-25 | USE OF ARABINOGALAKTAN IN CYCLE PRESERVATION MEDIA FOR CELL |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/621,489 US5897987A (en) | 1996-03-25 | 1996-03-25 | Use of arabinogalactan in cell cryopreservation media |
| US621,489 | 1996-03-25 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1997035472A1 WO1997035472A1 (en) | 1997-10-02 |
| WO1997035472B1 true WO1997035472B1 (en) | 1997-10-30 |
Family
ID=24490374
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1997/004764 WO1997035472A1 (en) | 1996-03-25 | 1997-03-25 | Use of arabinogalactan in cell cryopreservation media |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US5897987A (en) |
| EP (1) | EP0891134B1 (en) |
| JP (1) | JP4031044B2 (en) |
| CA (1) | CA2250084A1 (en) |
| DE (1) | DE69713312T2 (en) |
| WO (1) | WO1997035472A1 (en) |
Families Citing this family (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6378527B1 (en) * | 1998-04-08 | 2002-04-30 | Chondros, Inc. | Cell-culture and polymer constructs |
| WO1999060849A1 (en) | 1998-05-26 | 1999-12-02 | Lifecell Corporation | Cryopreservation of human red blood cells |
| DE19835368C1 (en) * | 1998-08-05 | 2000-02-10 | Juergen Steinmeyer | Process for the preparation of a cartilage cell suspension |
| GR1004204B (en) * | 1999-09-29 | 2003-09-05 | Cryopreserved amniotic human cells for future therapeutic, diagnostic, genetic and other uses | |
| US7112576B1 (en) | 1999-12-10 | 2006-09-26 | Regents Of The University Of Minnesota | Compositions and methods for cryopreservation of peripheral blood lymphocytes |
| WO2002054864A1 (en) * | 2001-01-12 | 2002-07-18 | Abs Corporation | Semen extender composition and methods for manufacturing and using |
| EP1475434A1 (en) * | 2003-05-09 | 2004-11-10 | Oncoscience AG | Method for storing tumor cells |
| US20070077237A1 (en) * | 2003-10-09 | 2007-04-05 | Udi Damari | Method for freezing, thawing and transplantation of viable cartilage |
| WO2005072790A1 (en) * | 2004-02-02 | 2005-08-11 | I.M.T. Interface Multigrad Technology Ltd. | Device for directional cooling of biological matter |
| JP4777908B2 (en) * | 2004-02-02 | 2011-09-21 | コア・ダイナミクス・リミテッド | Biological materials and methods and solutions for storage of biological materials |
| US7892726B2 (en) * | 2004-06-07 | 2011-02-22 | Core Dynamics Limited | Method for sterilizing lyophilized eukaryotic anuclear cells with gamma irradiation |
| US8037696B2 (en) * | 2004-08-12 | 2011-10-18 | Core Dynamics Limited | Method and apparatus for freezing or thawing of a biological material |
| WO2006090372A2 (en) * | 2005-02-22 | 2006-08-31 | I.M.T. Interface Multigrad Technology Ltd. | Preserved viable cartilage, method for its preservation, and system and devices used therefor |
| US8703198B2 (en) | 2005-03-02 | 2014-04-22 | Aquatrove Biosciences | Water-based personal moisturizers and lubricants, in particular vaginal lubricants, and uses thereof |
| US20100233295A1 (en) * | 2005-03-02 | 2010-09-16 | Vibha Gupta | Aqueous moisturizers and lubricants and uses thereof |
| SG161258A1 (en) * | 2005-06-21 | 2010-05-27 | Pervasis Therapeutics Inc | Methods and compositions for enhancing vascular access |
| WO2007015252A2 (en) * | 2005-08-03 | 2007-02-08 | I.M.T. Interface Multigrad Technology Ltd. | Somatic cells for use in cell therapy |
| US20070110666A1 (en) * | 2005-09-30 | 2007-05-17 | Paul Pevsner | Methods for preparation of live body tissues for examination |
| US20070098695A1 (en) * | 2005-10-31 | 2007-05-03 | Deolden James | Methods of islet separation during isolation |
| WO2007071048A1 (en) * | 2005-12-22 | 2007-06-28 | Jane Ennis | Viable cells from frozen umbilical cord tissue |
| US20070259327A1 (en) * | 2006-05-03 | 2007-11-08 | Bioverde. Inc. | Cryopreservation medium and cryopreservation method for tissues and cells |
| JP2008099659A (en) * | 2006-09-22 | 2008-05-01 | Ina Food Ind Co Ltd | Protective agent against environmental stress of microorganism, and fermented food and method for producing the same |
| US20080113432A1 (en) * | 2006-11-15 | 2008-05-15 | Mariposa Biotechnology, Inc. | Methods and compositions for reanimating cryopreserved oocytes |
| US20080113431A1 (en) * | 2006-11-15 | 2008-05-15 | Mariposa Biotechnology, Inc. | Methods and compositions for cryopreserving oocytes |
| US20090123436A1 (en) * | 2007-11-08 | 2009-05-14 | Surmodics, Inc. | Cryopreservative compositions and methods |
| US20090202978A1 (en) * | 2008-02-13 | 2009-08-13 | Ginadi Shaham | Method and apparatus for freezing of a biological material |
| WO2014130883A2 (en) | 2013-02-22 | 2014-08-28 | Allosource | Cartilage mosaic compositions and methods |
| WO2014150784A1 (en) | 2013-03-15 | 2014-09-25 | Allosource | Cell repopulated collagen matrix for soft tissue repair and regeneration |
| KR102138399B1 (en) | 2013-03-15 | 2020-07-27 | 알로소스 | Perforated osteochondral allograft compositions |
| ES2529265B1 (en) * | 2013-07-18 | 2015-10-07 | Universidad De Sevilla | TAC monitoring of cold preservation processes and cryopreservation of biological material |
| JP6373241B2 (en) * | 2015-10-05 | 2018-08-15 | 有限会社ジーエヌコーポレーション | How to store cells |
| US10004697B2 (en) | 2016-11-16 | 2018-06-26 | M Pharmaceutical Usa Inc. | In vivo sperm selection for treating male infertility |
| GB201708999D0 (en) | 2017-06-06 | 2017-07-19 | Univ Warwick | Recrystallization Inhibitor |
| WO2019156601A1 (en) * | 2018-02-08 | 2019-08-15 | Al Otaibi Noha Abdullah S | High efficiency cryo-additive agent to improve mammalian cells biopreservation |
| CN111034712A (en) * | 2019-12-23 | 2020-04-21 | 成都大熊猫繁育研究基地 | Panda milk preservation method |
| GB202007905D0 (en) * | 2020-05-27 | 2020-07-08 | Univ Edinburgh | Cryopressing macrophages |
| CN113615681A (en) * | 2021-08-27 | 2021-11-09 | 郑州源创吉因实业有限公司 | Frozen stock solution and frozen stock method for immune cells |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4327177A (en) * | 1969-04-10 | 1982-04-27 | Wallace Shrimpton | Method and means for controlling the sex of mammalian offspring and product therefor |
| US4007087A (en) * | 1975-10-17 | 1977-02-08 | Gametrics Limited | Sperm fractionation and storage |
| US4004975A (en) * | 1975-12-30 | 1977-01-25 | The United States Of America As Represented By The Secretary Of The Navy | Method of isolating and cryopreserving human white cells from whole blood |
| US4605558A (en) * | 1984-04-20 | 1986-08-12 | Wallace Shrimpton | Process for cell separation |
| US5490991A (en) * | 1986-07-03 | 1996-02-13 | Advanced Magnetics, Inc. | Directed delivery of radioprotectants using a receptor specific carrier |
| US5478576A (en) * | 1986-07-03 | 1995-12-26 | Advanced Magnetics, Inc. | Arabinogalactan derivatives and uses thereof |
| WO1992008347A1 (en) * | 1988-04-18 | 1992-05-29 | Cryolife, Inc. | Cryoprotective agent |
| US5071741A (en) * | 1988-04-18 | 1991-12-10 | Cryolife, Inc. | Cryoprotective agent and its use in cryopreservation of cellular matter |
| US5116969B1 (en) * | 1990-04-26 | 1997-04-01 | Larex International Inc | Ultrarefined arabinogalactan product |
| GB9009674D0 (en) * | 1990-04-30 | 1990-06-20 | Zaadunie Bv | Improvements in or relating to organic compounds |
| CA2135295A1 (en) * | 1992-06-17 | 1993-12-23 | Chu Jung | Arabinogalactan derivatives and uses thereof |
| US5405742A (en) * | 1993-07-16 | 1995-04-11 | Cyromedical Sciences, Inc. | Solutions for tissue preservation and bloodless surgery and methods using same |
| JPH07113001A (en) * | 1993-08-23 | 1995-05-02 | Tsumura & Co | Novel polysaccharide and radiation-preventing agent containing the polysaccharide as active ingredient |
-
1996
- 1996-03-25 US US08/621,489 patent/US5897987A/en not_active Expired - Lifetime
-
1997
- 1997-03-25 WO PCT/US1997/004764 patent/WO1997035472A1/en active IP Right Grant
- 1997-03-25 CA CA002250084A patent/CA2250084A1/en not_active Abandoned
- 1997-03-25 DE DE69713312T patent/DE69713312T2/en not_active Expired - Fee Related
- 1997-03-25 EP EP97916960A patent/EP0891134B1/en not_active Expired - Lifetime
- 1997-03-25 JP JP53454397A patent/JP4031044B2/en not_active Expired - Fee Related
-
1998
- 1998-08-21 US US09/138,101 patent/US6593138B1/en not_active Expired - Fee Related
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO1997035472B1 (en) | Use of arabinogalactan in cell cryopreservation media | |
| EP0790767B1 (en) | Wound repair dressings and methods for their preservation | |
| EP0511317B1 (en) | Composition to improve survival of biological materials | |
| JP4031044B2 (en) | Use of arabinogalactan in cell cryopreservation medium | |
| Harp et al. | Cryopreservation of murine ovarian tissue | |
| EP1093377B1 (en) | Inclusion of apoptotic regulators in solutions for cell storage at low temperature | |
| EP0237567B1 (en) | Perfusate for the preservation of organs | |
| EP1730523B1 (en) | Use of a composition which regulates oxidation/reduction reactions intracellularly and/or extracellularly in a staining or sorting process of spermatozoa | |
| NL193971C (en) | Perfusate for the preservation of organs. Aqueous composition for the preservation and preservation of organs. | |
| Vajta et al. | Morphological changes of in‐vitro‐produced bovine blastocysts after vitrification, in‐straw direct rehydration, and culture | |
| US6060233A (en) | Methods for the lyophilization of platelets, platelet membranes or erythrocytes | |
| CA2427765A1 (en) | Evaluation and preservation solution | |
| CA2235069A1 (en) | Methods and compositions to improve germ cell and embryo survival and function | |
| WO1991018505A1 (en) | Cryopreservation of cultured epithelial sheets | |
| JP2008501320A (en) | Cell preservation method | |
| WO2020207151A1 (en) | Cryopreservation solution without dmso, preparation method therefor and application thereof | |
| JPH07255469A (en) | Frozen storage solution for mammalian cell | |
| Song et al. | Vitreous preservation of rabbit articular cartilage | |
| JP2001247401A (en) | Cooling preservation liquid for tissue | |
| EP2578082A2 (en) | Composition for the cold storage of organs | |
| CA2207324A1 (en) | Organ transplant solutions and method for transplanting an organ | |
| Sławeta et al. | The effect of glutathione on the motility and fertility of frozen bull sperm | |
| GB1014712A (en) | Improvements in and relating to liquid media in contact with animal cells | |
| WO2008127959A1 (en) | Ergothioneine and/or its derivatives as a cell preservative | |
| US6040132A (en) | Methods for the lyophilization of living biological materials |