Classifications

• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
  • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
  • A61K31/716—Glucans
  • A61K31/718—Starch or degraded starch, e.g. amylose, amylopectin
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
  • A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents

Definitions

• This invention relates to an improved method of enhancing a population of one or more target microorganisms in the gastrointestinal tract, especially the small intestine and the large bowel, of animals and humans.
• the present invention consists in an improved method of enhancing a population of one or more target microorganisms in the gastrointestinal tract of an animal, the improvement comprising providing to the animal a selected modified or unmodified resistant starch or mixtures thereof, such that the one or more microorganisms will selectively utilise the starch and/or increase in number and/or activity in the gastrointestinal tract.
• the target population of microorganism may be enhanced throughout the gastrointestinal tract of the animal or targeted at specific sites of the gastrointestinal tract. It will be appreciated that the present invention will be suitable for any animal that requires alteration of its gastrointestinal flora. The present method is particularly suitable for use in humans.
• the starches suitable include resistant or high amylose starches and modified forms thereof. The animal or human may be fed the selected resistant starch or the starch may be incorporated in a probiotic composition.
• resistant starch includes those forms defined as RSl, RS2, RS3 and RS4 as defined in Brown, McNaught and
• resistant starch is a high amylose starch particularly high amylose starches as disclosed and taught in WO 94/03049 and WO 94/14342, the contents of which are incorporated into this specification for the purposes of convenient cross-reference.
• high amylose starches which are resistant starches and include maize starch having an amylose content of 50% w/w or more, particularly 80% w/w or more, rice or wheat starch having an amylose content of 27% w/w or more and; particular granular size ranges of starches having an amylose content of 50% or more and enhanced resistant starch content, these starches including maize, barley, and legumes.
• This invention is not, however, limited to these forms of resistant starch.
• other forms of resistant starch are derived from sources such as bananas and tubers such as potatoes and modified forms thereof.
• starch may be advantageous to also chemically modify the starch to, for instance, alter the charge density or hydrophobicity of the granule and/or granule surface to enhance the attachment compatibility between the microorganism and the resistant starch.
• Chemical modifications such as etherification, esterification, acidification and the like are well known in this art as being suitable chemical treatments.
• the conformation or structure of the starch can be altered. Examples include acid or enzyme thinning and cross bonding using difunctional reagents.
• the starches may be modified physically by, for example, crystallisation. It is also within the scope of this invention to subject enzymatically treated resistant starches to chemical modification as described above.
• Hi-maizeTM (trade mark) refers to a high amylose starch obtained from Starch Australasia Limited.
• Figure 1 shows comparison of the co-culturing of Lactobacillus acidophilus with Bifidobacte ⁇ um strain X8AT2 in glucose and amylose starch medium.
• Figure 2 shows enumeration of number of bifidobacteria in starch based medium inoculated with human faecal homogenates and incubated anaerobically at 37°C for 12 hours. Individual starches according to the description in Table 4.
• Figure 3 shows enumeration of number of amylolytic bacteria in starch based media inoculated with human faecal homogenates and incubated anaerobically at 37°C for 12 hours. Individual starches as in Table 4.
• Figure 4 shows enumeration of major bacterial groups in stomach contents from mice on various starch based diets (Table 4).
• Figure 5 shows enumeration of major bacterial groups in ileal contents from mice on various starch based diets (Table 4).
• Figure 6 shows enumeration of major bacterial groups caecal contents from mice on various starch based diets (Table 4)
• Figure 7 shows enumeration of major bacterial group in colon contents from mice on various starch based diets (Table 4).
• Figure 8 shows the total anaerobic microbial population of ileal origin, 9 hours post inoculation in media containing starch nos 4, 6, 8, 9 and glucose.
• Figure 9 shows the total anaerobic microbial population of caecal origin, 9 hours post inoculation in media containing starch nos 4. 6, 8. 9 and glucose.
• amylase activity of specific intestinal bacteria when grown in standard laboratory medium containing glucose, starch (amylopectin) or resistant starch (amylose) added to a defined medium (composition included in Table 1 at a final concentration of 10 mg/ml), one can show that many of the intestinal bacteria produce amylase which can utilise the resistant starch (Table 2).
• the specific growth rates when six different intestinal bacteria were grown on glucose, amylose, amylopectin, Hi-maizeTM and carboxymethylated resistant starch were determined (Table 3). The various bacteria tested grew at very different rates to each other, indicative that individual bacterial groups or species will be selectively enhanced by the form of starch used.
• Table 1 Composition of medium used for growing intestinal strains of bacteria.
• Lactobacillus sp in the amylose starch medium Lactobacillus sp in the amylose starch medium
• the growth of L ⁇ cf. acidophilus in the Hi-maizeTM containing medium with or without the present of Bif. X8AT2 was compared.
• the growth medium contained 1% Hi-maizeTM starch or glucose as the growth carbon and energy source.
• the medium was autoclaved at 121°C for 15 minutes and strictly anaerobic conditions were used during the medium preparation.
• Overnight cultures (0.1 ml) of Lact. acidophilus and Bif. X8AT2 were inoculated into the serum tubes containing either glucose or Hi- maizeTM starch based media.
• mice were fed either normal mouse diet or a prepared diet containing either waxy starch, Hi-maizeTM or modified Hi-maizeTM (carboxymethylated) and were orally dosed with 200 microlitres of Bifidobacte ⁇ um sp strain X8AT2 or Bifidobacte ⁇ um bifidum cultures.
• the composition of the mouse prepared diet is included in Table 6. Faecal samples were collected after continuous feeding from day 3 to day 8 of the diet plus the bifidobacteria. The major bacterial groups were enumerated using selective media and the total bacteria output for the groups were calculated.
• Wheat bran 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100
• Group A Waxy starch plus X8AT2 - Bifidobacteria human isolates:
• Group B Hi-rnaizeTM starch plus X8AT2;
• Group C Carboxymethylated resistant starch plus X8AT2;
• Group D Hi-maizeTM starch plus Bif. bifidum;
• Group E Normal mice diet plus X8AT2
• Group A received 40% waxy starch in the diet, and groups C and E had 40% modified starches D2 and D5 , respectively in their diets.
• Group D was the Hi-maizeTM starch group and group B was assigned as the control to be fed with normal mice diet.
• Two faecal samples were collected at the end of experimental period (4 weeks) to enumerate the population of Bifidobacterium by using propionic acid agar. Bifidobacteria were further identified by cell morphology under light microscopy. The population of Bifidobacterium was expressed as total output per day per mice.
• mice used in the experiment were free of detectable bifidobacteria ( ⁇ 10 3 ) and continued to be so for the 2 months as control animals (Table 8). It is very surprising, however, to find that when the mice shifted from normal mice diet to the starch diets, the population of bifidobacteria increased significantly. The degree of increase depended on the type of starch incorporated into the diets. Hi-maizeTM starch diet yielded the greatest numbers of native bifidobacteria in the mice faeces, followed by the waxy starch diet.
• Modified Hi-maizeTM starch D57 demonstrated better results in the stimulation of the growth of bifidobacteria than modified Hi- maizeTM starch D2.
• the statistical analysis of the data is also presented in Table 8.
• 200 ul of Bif. X8AT2 was orally dosed into mice for 5 days.
• Numbers oi Lactobacillus from all of groups were quantified in the mice faeces at both stages of experiments by using Rogosa agar.
• the cell morphology of Lactobacillus were also checked under phase-contract microscopy.
• mice All of the mice were heavily colonised with dense populations of Lactobacillus.
• the influence of diets on faecal population of Lactobacillus is shown in Table 9.
• none of the starch diets supported the increased growth of native Lactobacillus, in comparison with normal mice diets.
• Particularly low numbers of Lactobacillus were detected in the groups of mice fed with modified starches D2 and D57.
• the population of Lactobacillus however, increased in the group of mice fed with Hi-maizeTM diet when amylolytic bifidobacterial strain X8AT2 was associated with the mice.
• Group B Normal mice diet
• Group E Acetylated maize starch
• Lactobacillus Period 1 7.596 ⁇ 0.477 8.113 ⁇ 0.532 7.423 ⁇ 0.295 7.858 ⁇ 0.367 7.309 ⁇ 0.326
• the mixtures were incubated 37°C for 24 h and sampled at 0, 3, 6, 9, 12 and 24 h post inoculation.
• Starch no. 6 The difference in resistance to degradation was even more significant in cultures incubated for 12 h. At this point (12 h), six starches were assayed: Starch nos. 1, 4, 6, 7, 8 and 9. Starch no. 1 was of the starches the most easily degraded (2.74% remaining), followed by Starch no. 8 (5.3%). Starch no. 4 (23.4%), Starch no. 9 (44.5%), Starch no. 7 (79.2%) and Starch no. 6, the one most resistant to degradation. No degradation of starch no. 6 could be detected 12 h post inoculation (Table 10).
• Hi-maizeTM can be modified to various levels with chemical reagents, such as acetic anhydride.
• chemical reagents such as acetic anhydride.
• the degree of susceptibility to in vitro digestion by bacterial alpha-amylase and amyloglucosidase of Hi-maizeTM and three acetylated starches from Hi-MaizeTM was ascertained using the Megazyme Total Starch Assay Procedure (AA/AMG 6/95). Each starch was solubilised and the enzyme resistant "residue" recovered by centrifugation. The residue was then solubilised using DMSO and assayed as per the Megazyme resistant starch method. The results are shown in Table 11. Table 11. Resistance of acetylated Hi-maizeTM starch to amylase digestion
• n-butyric acid was greatest in media containing Starch no. 8, followed by Starch no. 4, Starch no. 5, Starch no. 2, Starch no. 6 and media containing Starch no. 10 (Table 13).
• Starch no. 8 followed by Starch no. 3, Starch no. 9, Starch no. 6, Starch no. 4 and media containing Starch no. 2.
• iso-butyric acid was greatest in media containing glucose, followed by Starch no. 7 and Starch no. 3. Iso-butyric acid could not be detected in cultures supplied with any other starches.
• Starch no. 8 promoted production of all major SCFA's (acetic, propionic and butyric acid), more that any of the other starch, that resulted in a butyric acid concentration that was about 1.5 times greater than for Starch no. 3 (Table 13).
• mice Specific pathogen free mice were fed synthetic diets consistent with those presented in Table 6 but using waxy starch and starches 4, 6 and 9 (Table 4). Five animals per group were used and maintained in the diet for 2 weeks. Animals were sacrificed and the gastrointestinal tract was collected. Contents from the stomach, ileum, caecum and colon were collected, weighed and stored on ice for processing within an hour. The major bacterial groups were enumerated using routine selective media. The groups include the obligate anaerobes, lactobacilli, enterococci, coliforms, amylolytic bacteria, clostridia and bifidobacteria.
• Amylolytic activity was assessed for isolates from the mice on the various diets by ranking the zone of clearance around colonies on agar plates prepared using either amylose (Sigma) of starches 4, 6 or 9. Results are presented in Figures 4, 5, 6 and 7. It can be seen in these figures that the different starches will induce altered levels of specific groups of microbes at different sites in the tract.
• starch 6 and 4 stimulate lactobacillus from the stomach, ileum and caecum; starch 9 stimulates bifidobacterium in all sites samples; starch 4 stimulates endospore forming populations such as the clostridia in all sites sampled and suppresses the bifidobacterial numbers as all sites sampled; starch 9 suppressed endospore forming populations in all regions sampled.
• Example 11
• mice colonised with human faecal homogenates were fed a commercial animal diet.
• Material from gastrointestinal of germ free mice colonised with human microbes gastric, ileal, caecal and colonic content
• Wilkins Charlgren broth (1/1000).
• the faecal microbial composition of the animal that served as a source for inoculum is presented in Table 15. Diluted material was used as the inoculum for the starch media (Table 1) continuing the different resistant starches in Table 4.
• the mice gastrointestinal content mixes were sampled at 0 and 9 h post inoculation. The use of different modifications of resistant starches or unmodified starches could be used to control specific populations at different sites.
• starch 9 induced higher levels of obligate anaerobes in the ileum than were induced by starch 8 ( Figure 8) while starch 8 promoted higher levels of these obligate anaerobes in the caecum than were induced by starch 9 ( Figure 9).
• the resident bifidobacterial and amylolytic population may be replaced with new bfidobacterial and amylolytic populations. This will happen if the unmodfified Hi-maizeTM (Starch no. 6) is supplied. Although bifidobacterial and amylolytic populations will be disadvantaged in the short term, animals fed Starch 6 (fro about 2.5 months) have a dense bifidobacterial and amylolytic population through out the gastrointestinal tract ( Figures 4, 5, 6 and 7 and Table 14).
• modified resistant starches may lead to enhancement of other beneficial bacteria in the large intestine. Consequently, one can use a modified resistant starch in the diet to achieve one or all of the following conditions: i) as a general gut microflora stabiliser: ii) in clinical conditions related to disturbances e.g. flora related irritable bowel syndrome and inflammatory bowel disease, Crohn's disease, diarrhoea; iii) improved intestinal health e.g.
• resistant starch ingestion can cause a lowering of the pH which will lead to suppression of bacterial transformation of cholesterol and bile acids, thus affecting excretion of cholesterol and bile acids. Since the present inventors have found that modification of the resistant starch affected utilisation by specific microbes and the bacterial groups that were enhanced, modifications of the resistant starch could influence cholesterol and bile acid excretion levels.

Landscapes

• Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Medicinal Chemistry (AREA)
• Pharmacology & Pharmacy (AREA)
• Animal Behavior & Ethology (AREA)
• General Health & Medical Sciences (AREA)
• Public Health (AREA)
• Veterinary Medicine (AREA)
• Molecular Biology (AREA)
• Epidemiology (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Chemical Kinetics & Catalysis (AREA)
• General Chemical & Material Sciences (AREA)
• Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
• Organic Chemistry (AREA)
• Engineering & Computer Science (AREA)
• Nutrition Science (AREA)
• Coloring Foods And Improving Nutritive Qualities (AREA)
• Micro-Organisms Or Cultivation Processes Thereof (AREA)
• Medicines Containing Material From Animals Or Micro-Organisms (AREA)
• Medicines Containing Plant Substances (AREA)
• Jellies, Jams, And Syrups (AREA)
• Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
• Preparation Of Compounds By Using Micro-Organisms (AREA)
• Fodder In General (AREA)
• Medicinal Preparation (AREA)

Priority Applications (8)

Applications Claiming Priority (2)

Related Child Applications (2)

Publications (1)

Family

ID=3793122

Family Applications (1)

Country Status (11)

Cited By (7)

Families Citing this family (14)

Citations (4)

Family Cites Families (13)

• 1996
  • 1996-03-20 AU AUPN8809A patent/AUPN880996A0/en not_active Abandoned
• 1997
  • 1997-03-20 CA CA002253364A patent/CA2253364C/en not_active Expired - Fee Related
  • 1997-03-20 WO PCT/AU1997/000175 patent/WO1997034592A1/en active IP Right Grant
  • 1997-03-20 EP EP97908077A patent/EP0910359B1/de not_active Expired - Lifetime
  • 1997-03-20 AT AT97908077T patent/ATE347882T1/de not_active IP Right Cessation
  • 1997-03-20 JP JP53298197A patent/JP4128219B2/ja not_active Expired - Fee Related
  • 1997-03-20 DE DE69737096T patent/DE69737096T2/de not_active Expired - Lifetime
  • 1997-03-20 NZ NZ331952A patent/NZ331952A/en unknown
  • 1997-03-20 KR KR1019980707462A patent/KR20000064730A/ko not_active Application Discontinuation
  • 1997-03-20 ES ES97908077T patent/ES2278389T3/es not_active Expired - Lifetime
  • 1997-03-20 US US09/155,115 patent/US6274567B1/en not_active Expired - Lifetime
• 2001
  • 2001-05-18 US US09/859,540 patent/US6528498B2/en not_active Expired - Lifetime

Patent Citations (4)

Non-Patent Citations (1)

Also Published As

Similar Documents

Legal Events