WO1997034138B1 - Method of and apparatus for transferring micro quantities of liquid samples to discrete locations - Google Patents
Method of and apparatus for transferring micro quantities of liquid samples to discrete locationsInfo
- Publication number
- WO1997034138B1 WO1997034138B1 PCT/US1997/004015 US9704015W WO9734138B1 WO 1997034138 B1 WO1997034138 B1 WO 1997034138B1 US 9704015 W US9704015 W US 9704015W WO 9734138 B1 WO9734138 B1 WO 9734138B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- cells
- chamber
- pressure
- conduits
- gas pressure
- Prior art date
Links
- 239000007788 liquid Substances 0.000 title claims abstract 13
- 238000007789 sealing Methods 0.000 claims 6
- 230000001939 inductive effect Effects 0.000 claims 3
- 230000003247 decreasing Effects 0.000 claims 1
- 210000001736 Capillaries Anatomy 0.000 abstract 2
- 239000011521 glass Substances 0.000 abstract 1
- 238000003780 insertion Methods 0.000 abstract 1
Abstract
A method of and apparatus for simultaneously and uniformly transferring micro quantities of liquid from a first plurality of separate cells to a second plurality of separate cells. The gel loading apparatus (20) comprises a vertical support plate (26), a pressure block (28) mounted on the support plate and defining a pressure block (28) mounted on the support plate and defining pressure chamber (30) therein, and a slidable pressure tray (32) engageable with an underside of the pressure block for closing the pressure chamber. A plurality of liquid conveying conduits (capillaries 34) have first ends (36) inside the pressure chamber (30) and second ends (38) outside the pressure chamber. The second ends (38) of the capillaries (34) are preferably arranged for insertion between the plates of glass (22, 24) and into destination wells (21).
Claims
1. A method of simultaneously transferring quantities of liquid from a first plurality of separate cells to a second plurality of separate cells, each of the first plurality of separate cells containing a liquid sample, the method comprising: placing intake ends of a plurality of conduits into the first cells so that the intake ends of the conduits are immersed in the liquid samples, the conduits further including discharge ends; aligning the discharge ends of the conduits with the second plurality of cells; and creating a pressure differential between the first plurality of cells and the second plurality of cells to cause quantities of the liquid samples to simultaneously flow through their respective conduits and to the second plurality of cells.
2. A method as set forth in claim 1 further comprising the step of sealing one of said first and second plurality of cells in a chamber, the other of said first and second plurality of cells being exterior of the chamber, and wherein the step of creating a pressure differential comprises inducing a change of gas pressure in the chamber so that the gas pressure in the chamber is different from gas pressure in the other of said first and second plurality of cells.
3. A method as set forth in claim 2 wherein: the discharge ends of the conduits are within the chamber; the step of sealing one of said first and second plurality of cells in a chamber comprises sealing the second plurality of cells in the chamber; and the step of inducing a change of gas pressure in the chamber comprises decreasing gas pressure in the chamber to a pressure less than gas pressure in the first plurality of cells to draw liquid through the conduits and into the first plurality of cells.
4. A method as set forth in claim 2 wherein: the intake ends of the conduits are within the chamber and the liquid samples have surfaces exposed to the interior of the chamber; the step of sealing one of said first and second plurality of cells in a chamber comprises sealing the first plurality of cells in the chamber; and the step of inducing a change of gas pressure in the chamber comprises increasing gas pressure in the chamber to a pressure greater than gas pressure in the second plurality of cells to force liquid through the conduits and into the second plurality of cells.
5. A method as set forth in claim 1 wherein the step of creating a pressure differential comprises simultaneously pressurizing the first plurality of cells with a gas to pressurize the liquid samples and cause micro quantities of the liquid samples to flow through their respective conduits and to the second plurality of cells.
6. A method as set forth in claim 5 wherein the step of simultaneously pressurizing the first plurality of cells comprises simultaneously pressurizing each of the first plurality of cells to substantially the same pressure.
7. A method as set forth in claim 6 further comprising the step of sealing the first plurality of cells in a pressure chamber, the intake ends of the conduits being within the pressure chamber and the discharge ends of the conduits being exterior of the pressure chamber, the liquid samples having surfaces exposed to the interior of the pressure chamber, and wherein the step of simultaneously pressurizing the plurality of cells comprises pressurizing gas within the pressure chamber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU23252/97A AU2325297A (en) | 1996-03-15 | 1997-03-14 | Method of and apparatus for transferring micro quantities of liquid samples to discrete locations |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/617,494 | 1996-03-15 | ||
| US08/617,494 US5849598A (en) | 1996-03-15 | 1996-03-15 | Method for transferring micro quantities of liquid samples to discrete locations |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1997034138A1 WO1997034138A1 (en) | 1997-09-18 |
| WO1997034138B1 true WO1997034138B1 (en) | 1997-10-16 |
Family
ID=24473863
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1997/004015 WO1997034138A1 (en) | 1996-03-15 | 1997-03-14 | Method of and apparatus for transferring micro quantities of liquid samples to discrete locations |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US5849598A (en) |
| AU (1) | AU2325297A (en) |
| WO (1) | WO1997034138A1 (en) |
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- 1996-03-15 US US08/617,494 patent/US5849598A/en not_active Expired - Fee Related
-
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- 1997-03-14 WO PCT/US1997/004015 patent/WO1997034138A1/en active Application Filing
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