WO1997021801A3 - Production and screening process for phytochemical production by plant cell cultures - Google Patents

Production and screening process for phytochemical production by plant cell cultures Download PDF

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Publication number
WO1997021801A3
WO1997021801A3 PCT/US1996/019724 US9619724W WO9721801A3 WO 1997021801 A3 WO1997021801 A3 WO 1997021801A3 US 9619724 W US9619724 W US 9619724W WO 9721801 A3 WO9721801 A3 WO 9721801A3
Authority
WO
WIPO (PCT)
Prior art keywords
production
phytochemical
plant cell
cell cultures
screening process
Prior art date
Application number
PCT/US1996/019724
Other languages
French (fr)
Other versions
WO1997021801A2 (en
Inventor
Amlan Dutta
Ramesh C Pandey
Original Assignee
Xechem Inc
Amlan Dutta
Ramesh C Pandey
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xechem Inc, Amlan Dutta, Ramesh C Pandey filed Critical Xechem Inc
Priority to AU22398/97A priority Critical patent/AU2239897A/en
Priority to KR1019980704381A priority patent/KR19990072075A/en
Publication of WO1997021801A2 publication Critical patent/WO1997021801A2/en
Publication of WO1997021801A3 publication Critical patent/WO1997021801A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D305/00—Heterocyclic compounds containing four-membered rings having one oxygen atom as the only ring hetero atoms
    • C07D305/14—Heterocyclic compounds containing four-membered rings having one oxygen atom as the only ring hetero atoms condensed with carbocyclic rings or ring systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/337—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having four-membered rings, e.g. taxol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0025—Culture media for plant cell or plant tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P15/00—Preparation of compounds containing at least three condensed carbocyclic rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

A process for producing phytochemicals from plant cell cultures is provided. That process includes the step of culturing plant cells that produce a particular phytochemical in a multi-phase culture medium that includes an aqueous phase and a hydrophobic phase having a high partition coefficient for paclitaxel relative to the aqueous phase. The phytochemical is recovered from the hydrophobic phase. A process of screening plant cell cultures to plant cell lines that produce a particular phytochemical using the production process is also provided. A preferred phytochemical is paclitaxel.
PCT/US1996/019724 1995-12-12 1996-12-11 Production and screening process for phytochemical production by plant cell cultures WO1997021801A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU22398/97A AU2239897A (en) 1995-12-12 1996-12-11 Production and screening process for phytochemical production by plant cell cultures
KR1019980704381A KR19990072075A (en) 1995-12-12 1996-12-11 Production and screening methods for producing plant chemicals by plant cell culture

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US57116695A 1995-12-12 1995-12-12
US08/571,166 1995-12-12

Publications (2)

Publication Number Publication Date
WO1997021801A2 WO1997021801A2 (en) 1997-06-19
WO1997021801A3 true WO1997021801A3 (en) 1997-08-14

Family

ID=24282577

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/019724 WO1997021801A2 (en) 1995-12-12 1996-12-11 Production and screening process for phytochemical production by plant cell cultures

Country Status (5)

Country Link
KR (1) KR19990072075A (en)
CN (1) CN1209168A (en)
AU (1) AU2239897A (en)
CA (1) CA2242929A1 (en)
WO (1) WO1997021801A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW200304947A (en) * 2002-02-08 2003-10-16 Bristol Myers Squibb Co Compositions and methods for latering biosynthesis of taxanes and taxane-related compounds
DE102006006269A1 (en) * 2006-02-10 2007-08-23 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Method and culture device for culturing biological cells
DK3077523T3 (en) * 2013-12-02 2020-07-20 Phyton Holdings Llc Production of Thapsia Argins Using Thapsia Cell Suspension Culture
CN105349592B (en) * 2015-12-09 2018-11-27 天津艾赛博生物技术有限公司 The method of the complementary plant cell miscegenation culture production secondary metabolite of metabolism

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3138493A1 (en) * 1981-09-28 1983-04-07 Rolf 6902 Sandhausen Beiderbeck A process for obtaining lipophilic metabolic products from suspension cultures of live plant cells
WO1990000193A1 (en) * 1988-06-29 1990-01-11 Plantoma Corporation Sustained autotrophic production of plant secondary metabolites
US5019504A (en) * 1989-03-23 1991-05-28 The United States Of America As Represented By The Secretary Of Agriculture Production of taxol or taxol-like compounds in cell culture
EP0577274A2 (en) * 1992-06-11 1994-01-05 Nippon Oil Co., Ltd. Process for preparing taxane compounds
EP0683232A1 (en) * 1993-11-15 1995-11-22 Mitsui Petrochemical Industries, Ltd. Process for producing taxane diterpene and method of harvesting cultred cell capable of producing taxane diterpene in high yield

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3138493A1 (en) * 1981-09-28 1983-04-07 Rolf 6902 Sandhausen Beiderbeck A process for obtaining lipophilic metabolic products from suspension cultures of live plant cells
WO1990000193A1 (en) * 1988-06-29 1990-01-11 Plantoma Corporation Sustained autotrophic production of plant secondary metabolites
US5019504A (en) * 1989-03-23 1991-05-28 The United States Of America As Represented By The Secretary Of Agriculture Production of taxol or taxol-like compounds in cell culture
EP0577274A2 (en) * 1992-06-11 1994-01-05 Nippon Oil Co., Ltd. Process for preparing taxane compounds
EP0683232A1 (en) * 1993-11-15 1995-11-22 Mitsui Petrochemical Industries, Ltd. Process for producing taxane diterpene and method of harvesting cultred cell capable of producing taxane diterpene in high yield

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BASSETTI L. AND TRAMPER J.: "Extractive biocatalysis in a two-liquid-phase system with plant cell suspensions", PHARMACY WORLD AND SCIENCE, vol. 15, no. 6, 17 December 1993 (1993-12-17), pages Sup. H5, XP000674600 *
BYUN S. AND PETERSEN H.: "Two-phase airlift fermentor operation with elicitation for the enhanced production of benzophenanthridine alkaloids in cell suspensions of Eschscholtzia californica", BIOTECHNOLOGY AND BIOENGINEERING, vol. 44, no. 1, 5 June 1994 (1994-06-05), pages 14 - 20, XP002032586 *
COLLINS M. ET AL.: "The production of taxol using two-phase plant cell culture; Taxus sp. suspension cell culture in the presence of two-phase system", ABSTR. PAP. AM. CHEM. SOC., vol. 207, no. Pt.2, 1994, pages BTEC25, XP000675547 *
COLLINS-PAVAO M. ET AL.: "Taxol partitioning in two-phase plant cell cultures of Taxus brevifolia", JOURNAL OF BIOTECHNOLOGY, vol. 49, no. 1-3, 20 August 1996 (1996-08-20), pages 95 - 100, XP002032590 *
DUTTA A. ET AL.: "Two-phase culture system for plant cells", BIOCHEMICAL ENGINEERING, vol. 745, 1994, pages 251 - 260, XP000674603 *
FETT-NETO A. ET AL.: "Improved growth and taxol yield in developing calli of Taxus cuspidata by medium composition modification", BIO/TECHNOLOGY, vol. 11, June 1993 (1993-06-01), pages 731 - 734, XP002032589 *
FETT-NETO A. ET AL.: "Improved taxol yield by aromatic carboxylic acid and amino acid feeding to cell cultures of Taxus cuspidata", BIOTECHNOLOGY AND BIOENGINEERING, vol. 44, no. 8, October 1994 (1994-10-01), pages 967 - 971, XP002032588 *

Also Published As

Publication number Publication date
WO1997021801A2 (en) 1997-06-19
AU2239897A (en) 1997-07-03
KR19990072075A (en) 1999-09-27
CA2242929A1 (en) 1997-06-19
CN1209168A (en) 1999-02-24

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