WO1996036717A3 - Dna sequences encoding a lycopene cyclase, antisense sequences derived therefrom and their use for the modification of carotenoids levels in plants - Google Patents

Dna sequences encoding a lycopene cyclase, antisense sequences derived therefrom and their use for the modification of carotenoids levels in plants Download PDF

Info

Publication number
WO1996036717A3
WO1996036717A3 PCT/EP1996/002101 EP9602101W WO9636717A3 WO 1996036717 A3 WO1996036717 A3 WO 1996036717A3 EP 9602101 W EP9602101 W EP 9602101W WO 9636717 A3 WO9636717 A3 WO 9636717A3
Authority
WO
WIPO (PCT)
Prior art keywords
plants
modification
derived therefrom
lycopene cyclase
dna sequences
Prior art date
Application number
PCT/EP1996/002101
Other languages
French (fr)
Other versions
WO1996036717A2 (en
Inventor
Marcel Kuntz
Original Assignee
Centre Nat Rech Scient
Marcel Kuntz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre Nat Rech Scient, Marcel Kuntz filed Critical Centre Nat Rech Scient
Priority to AU58977/96A priority Critical patent/AU5897796A/en
Publication of WO1996036717A2 publication Critical patent/WO1996036717A2/en
Publication of WO1996036717A3 publication Critical patent/WO1996036717A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention relates to DNA constructs comprising a DNA sequence homologous to some or all of a sequence encoding a lycopene cyclase, and to their use for modifying carotenoids levels in plants.
PCT/EP1996/002101 1995-05-17 1996-05-17 Dna sequences encoding a lycopene cyclase, antisense sequences derived therefrom and their use for the modification of carotenoids levels in plants WO1996036717A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU58977/96A AU5897796A (en) 1995-05-17 1996-05-17 Dna sequences encoding a lycopene cyclase, antisense sequenc es derived therefrom and their use for the modification of c arotenoids levels in plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP95401151 1995-05-17
EP95401151.6 1995-05-17

Publications (2)

Publication Number Publication Date
WO1996036717A2 WO1996036717A2 (en) 1996-11-21
WO1996036717A3 true WO1996036717A3 (en) 1996-12-27

Family

ID=8221491

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1996/002101 WO1996036717A2 (en) 1995-05-17 1996-05-17 Dna sequences encoding a lycopene cyclase, antisense sequences derived therefrom and their use for the modification of carotenoids levels in plants

Country Status (2)

Country Link
AU (1) AU5897796A (en)
WO (1) WO1996036717A2 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5792903A (en) * 1993-10-25 1998-08-11 Yissum Research Development Company Of Hebrew University Of Jerusalem Lycopene cyclase gene
US6429356B1 (en) * 1996-08-09 2002-08-06 Calgene Llc Methods for producing carotenoid compounds, and specialty oils in plant seeds
US6653530B1 (en) 1998-02-13 2003-11-25 Calgene Llc Methods for producing carotenoid compounds, tocopherol compounds, and specialty oils in plant seeds
WO1999055887A2 (en) * 1998-04-24 1999-11-04 E.I. Du Pont De Nemours And Company Carotenoid biosynthesis enzymes
JP2002517187A (en) * 1998-06-02 2002-06-18 ユニバーシティ オフ メリーランド Carotenoid biosynthesis and metabolism genes and methods of use
US6252141B1 (en) * 1998-08-14 2001-06-26 Yissum Research And Development Company Of The Hebrew University Of Jerusalem Tomato gene B polynucleotides coding for lycopene cyclase
US6232530B1 (en) 1998-11-30 2001-05-15 University Of Nevada Marigold DNA encoding beta-cyclase
DE19909637A1 (en) * 1999-03-05 2000-09-07 Peter Beyer Process for improving the agronomic value and nutritional properties of plants
JP2003527077A (en) 1999-04-15 2003-09-16 カルジーン エルエルシー Nucleic acid sequence of protein involved in tocopherol synthesis
US6872815B1 (en) 2000-10-14 2005-03-29 Calgene Llc Nucleic acid sequences to proteins involved in tocopherol synthesis
AU9052201A (en) 2000-08-07 2002-02-18 Monsanto Technology Llc Methyl-d-erythritol phosphate pathway genes
US7161061B2 (en) 2001-05-09 2007-01-09 Monsanto Technology Llc Metabolite transporters
ES2318004T3 (en) 2001-05-09 2009-05-01 Monsanto Technology Llc TYRA GENES AND USES OF THE SAME.
US7244877B2 (en) 2001-08-17 2007-07-17 Monsanto Technology Llc Methyltransferase from cotton and uses thereof
DE60235252D1 (en) 2001-10-25 2010-03-18 Monsanto Technology Llc AROMATIC METHYLTRANSFERASES AND THEIR USE
WO2003080647A2 (en) 2002-03-19 2003-10-02 Monsanto Technology, Llc Homogentisate prenyl transferase ('hpt') nucleic acids and polypeptides, and uses thereof
CN1681928A (en) 2002-08-05 2005-10-12 孟山都技术公司 Tocopherol biosynthesis related genes and uses thereof
KR101209345B1 (en) * 2002-12-06 2012-12-07 델몬트 후레쉬 프로듀스 컴퍼니 Transgenic pineapple plants with modified carotenoid levels and methods of their production

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0393690A1 (en) * 1989-04-21 1990-10-24 Kirin Beer Kabushiki Kaisha DNA sequences useful for the synthesis of carotenoids
WO1991009128A1 (en) * 1989-12-13 1991-06-27 Imperial Chemical Industries Plc Dna, constructs, cells and plants derived therefrom
WO1991013078A1 (en) * 1990-03-02 1991-09-05 Amoco Corporation Biosynthesis of carotenoids in genetically engineered hosts
JPH07155189A (en) * 1993-10-25 1995-06-20 Yissum Res Dev Co Of Hebrew Univ Of Jerusalem Lycopene cyclase
WO1995023863A1 (en) * 1994-03-01 1995-09-08 Centre National De La Recherche Scientifique (Cnrs) Dna constructs, cells and plants derived therefrom
WO1996028014A1 (en) * 1995-03-07 1996-09-19 Yissum Research Development Company Of The Hebrew University Of Jerusalem Lycopene cyclase gene

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0393690A1 (en) * 1989-04-21 1990-10-24 Kirin Beer Kabushiki Kaisha DNA sequences useful for the synthesis of carotenoids
WO1991009128A1 (en) * 1989-12-13 1991-06-27 Imperial Chemical Industries Plc Dna, constructs, cells and plants derived therefrom
WO1991013078A1 (en) * 1990-03-02 1991-09-05 Amoco Corporation Biosynthesis of carotenoids in genetically engineered hosts
JPH07155189A (en) * 1993-10-25 1995-06-20 Yissum Res Dev Co Of Hebrew Univ Of Jerusalem Lycopene cyclase
WO1995023863A1 (en) * 1994-03-01 1995-09-08 Centre National De La Recherche Scientifique (Cnrs) Dna constructs, cells and plants derived therefrom
WO1996028014A1 (en) * 1995-03-07 1996-09-19 Yissum Research Development Company Of The Hebrew University Of Jerusalem Lycopene cyclase gene

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
BOUVIER, F., ET AL.: "Xanthophyll biosynthesis in chromoplasts: isolation and molecular cloning of an enzyme catalyzing the conversion of 5,6-epoxycarotenoid into ketocarotenoid", PLANT JOURNAL, vol. 6, July 1994 (1994-07-01), pages 45 - 54, XP002017248 *
BRAMLEY, P., ET AL.: "BIOCHEMICAL CHARACTERIZATION OF TRANSGENIC TOMATO PLANTS IN WHICH CAROTENOID SYNTHESIS HAS BEEN INHIBITED THROUGH THE EXPRESSION OF ANTISENSE RNA TO PTOM5", THE PLANT JOURNAL, vol. 2, no. 3, pages 343 - 349, XP002017201 *
CAMARA B., ET AL.: "DEMONSTRATION AND SOLUBILIZATION OF LYCOPENE CYCLASE FROM CAPSICUM CHROMOPLAST MEMBRANES", PLANT PHYSIOL (BETHESDA) 80 (1). 1986. 172-174., XP002017199 *
DATABASE WPI Section Ch Week 9533, Derwent World Patents Index; Class C06, AN 95-250738, XP002017205 *
DERUÈRE. J., ET AL.: "Structure and expression of two plant genes encoding chromoplast-specific proteins: occurrence of partially spliced transcripts", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 199, no. 3, 1994, pages 1144 - 1150, XP002017485 *
EMBL SEQUENCE DATABASE ACC. NO. L40176, REL.43, 15-APR-1995. ARABIDOPSIS THALIANA LYCOPENE CYCLASE (LYC) MRNA, COMPLETE CDS. *
FRAY, R.G., ET AL.: "IDENTIFICATION AND GENETIC ANALYSIS OF NORMAL AND MUTANT PHYTOENE SYNTHASE GENES OF TOMATO BY SEQUENCING, COMPLEMENTATION AND CO-SUPPRESSION", PLANT MOLECULAR BIOLOGY, vol. 22, pages 589 - 602, XP002017200 *
HUGUENEY P., ET AL.: "Metabolism of cyclic carotenoids: A model for the alteration of this biosynthetic pathway in Capsicum annuum chromoplasts.", PLANT JOURNAL 8 (3). 1995. 417-424., September 1995 (1995-09-01), XP002017198 *
MISAWA, N., ET AL.: "EXPRESSION OF AN ERWINIA PHYTOENE DESATURASE GENE NOT OLNLY CONFERS MULTIPLE RESISTANCE TO HERBICIDES INTERFERING EITH CAROTENOID BIOSYNTHESIS BUT ALSO ALTERS XANTHOPHYLL METABOLISM IN TRANSGENIC PLANTS", THE PLANT JOURNAL, vol. 6, no. 4, pages 481 - 489, XP002017203 *
MISAWA, N., ET AL.: "FUNCTIONAL EXPRESSION OF THE ERWINIA UREDOVORA CAROTENOID BIOSYNTHESIS GENE CRTI IN TRANSGENIC PLANTS SHOWING AN INCREASE OF BETA-CAROTENE BIOSYNTHESIS ACTIVITY AND RESISTANCE TO THE BLEACHING HERBICIDE NORFLURAZON", THE PLANT JOURNAL, vol. 4, no. 5, pages 833 - 840, XP002017202 *

Also Published As

Publication number Publication date
WO1996036717A2 (en) 1996-11-21
AU5897796A (en) 1996-11-29

Similar Documents

Publication Publication Date Title
WO1996036717A3 (en) Dna sequences encoding a lycopene cyclase, antisense sequences derived therefrom and their use for the modification of carotenoids levels in plants
CA2296769A1 (en) Modified dorsal tissue affecting factor and compositions
AU2795292A (en) Synthetic dna sequence having enhanced insecticidal activity in maize
MX9606013A (en) Phosphonyldipeptides useful in the treatment of cardiovascular diseases.
WO1999049064A3 (en) Plant control genes
WO1992013950A3 (en) NUCLEOTIDIC SEQUENCES CODING FOR VARIABLE REGIONS OF β CHAINS OF HUMAN T LYMPHOCYTE RECEPTORS, CORRESPONDING PEPTIDIC SEGMENTS AND DIAGNOSTIC AND THERAPEUTIC APPLICATIONS
CA2153254A1 (en) Cloning of enterokinase and method of use
WO1994005791A3 (en) Dorsal tissue affecting factor and compositions
IL108719A0 (en) Expression constructs containing hiv inhibitory antisense and other nucleotide sequences, retroviralvectors and recombinant retroviruses containing them
WO2002060935A3 (en) A chimeric protein comprising non-toxic pseudomonas exotoxin a and type iv pilin sequences
AU6244296A (en) Novel beta-xylosidase, nucleotide sequence encoding it, and use thereof
AU6724794A (en) GRB3-3 gene, its variants and their uses
DE69422928T2 (en) MODIFIED MAXADILAN PROTEIN, THEIR PRODUCTION AND USE, AND DNA ENCODING THE PROTEIN
WO2002063022A3 (en) Plant regulatory sequences
WO2000022145A3 (en) A hydroperoxide lyase gene from maize and methods of use
WO2001030990A3 (en) Isolated nucleic acid sequence conferring salt tolerance in rice plant
WO2001095946A3 (en) Combination product for carrying out a cytotoxic treatment in a mammal
WO2002087604A8 (en) Preparations containing a combination of vitamin e and afamin
WO1996005300A3 (en) Or-1 on orphan receptor belonging to the nuclear receptor family
WO2003066852A3 (en) Plant growth modulation by drl1 and other proteins of the elongator complex
AU6125496A (en) Human growth factors, nucleotide sequence encoding growth factors, and methods of use thereof
WO2001092468A3 (en) Novel compositions for the expression of the human peptide histidine transporter 1 and methods of use thereof
AU6844596A (en) Nucleotide sequence encoding the cd33-like protein
AU7293296A (en) Xylanase, oligonucleotidic sequence encoding it and its uses
AU7942498A (en) Compositions and methods for sedating, anaesthetising and euthanising aquatic organisms

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AT AU AZ BB BG BR BY CA CH CN CZ DE DK EE ES FI GB GE HU IS JP KE KG KP KR KZ LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK TJ TM TR TT UA UG US UZ VN AM AZ BY KG KZ MD RU

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): KE LS MW SD SZ UG AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN

AK Designated states

Kind code of ref document: A3

Designated state(s): AL AM AT AU AZ BB BG BR BY CA CH CN CZ DE DK EE ES FI GB GE HU IS JP KE KG KP KR KZ LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK TJ TM TR TT UA UG US UZ VN AM AZ BY KG KZ MD RU

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): KE LS MW SD SZ UG AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

NENP Non-entry into the national phase

Ref country code: CA

122 Ep: pct application non-entry in european phase