WO1996027676A3 - Method and means for the production of gene products, novel recombinant dna vectors therefor and kits employing them - Google Patents

Method and means for the production of gene products, novel recombinant dna vectors therefor and kits employing them Download PDF

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Publication number
WO1996027676A3
WO1996027676A3 PCT/US1996/002849 US9602849W WO9627676A3 WO 1996027676 A3 WO1996027676 A3 WO 1996027676A3 US 9602849 W US9602849 W US 9602849W WO 9627676 A3 WO9627676 A3 WO 9627676A3
Authority
WO
WIPO (PCT)
Prior art keywords
recombinant dna
production
gene products
dna vectors
novel recombinant
Prior art date
Application number
PCT/US1996/002849
Other languages
French (fr)
Other versions
WO1996027676A2 (en
Inventor
Daniel Zurr
Zehava Grossman
Yaron Nophar
Original Assignee
Q B I Enterprises Ltd
Expression System Inc
Daniel Zurr
Zehava Grossman
Yaron Nophar
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Q B I Enterprises Ltd, Expression System Inc, Daniel Zurr, Zehava Grossman, Yaron Nophar filed Critical Q B I Enterprises Ltd
Priority to AU51795/96A priority Critical patent/AU5179596A/en
Publication of WO1996027676A2 publication Critical patent/WO1996027676A2/en
Publication of WO1996027676A3 publication Critical patent/WO1996027676A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/4753—Hepatocyte growth factor; Scatter factor; Tumor cytotoxic factor II
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/80—Vectors comprising a special translation-regulating system from vertebrates
    • C12N2840/85—Vectors comprising a special translation-regulating system from vertebrates mammalian

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A recombinant DNA vector or vector system collectively comprising a gene encoding a TIF of a eukaryotic translation factor (ETF), preceded by a promoter wherein the promoter is a weak eukaryotic promoter; and at least one internal ribosone entry site (IRES) region, followed by a cloning site into which at least one gene encoding a polypeptide to be produced can be inserted.
PCT/US1996/002849 1995-03-03 1996-02-29 Method and means for the production of gene products, novel recombinant dna vectors therefor and kits employing them WO1996027676A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU51795/96A AU5179596A (en) 1995-03-03 1996-02-29 Method and means for the production of gene products, novel recombinant dna vectors therefor and kits employing them

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IL11287295A IL112872A0 (en) 1995-03-03 1995-03-03 Novel recombinant dna vectors and kits employing them
IL112872 1995-03-03

Publications (2)

Publication Number Publication Date
WO1996027676A2 WO1996027676A2 (en) 1996-09-12
WO1996027676A3 true WO1996027676A3 (en) 1996-10-31

Family

ID=11067171

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/002849 WO1996027676A2 (en) 1995-03-03 1996-02-29 Method and means for the production of gene products, novel recombinant dna vectors therefor and kits employing them

Country Status (3)

Country Link
AU (1) AU5179596A (en)
IL (1) IL112872A0 (en)
WO (1) WO1996027676A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6933378B2 (en) 1997-05-30 2005-08-23 Joseph Atabekov Methods for coexpression of more than one gene in eukaryotic cells
FI972293A (en) 1997-05-30 1998-12-01 Joseph Atabekov Methods for co-expression of more than one gene
WO2013163346A1 (en) * 2012-04-25 2013-10-31 The Regents Of The University Of California Synthetic promoter for modulating gene expression

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990001550A1 (en) * 1988-07-29 1990-02-22 Zymogenetics, Inc. High efficiency translation of polycistronic messages in eucaryotic cells
DE4206769A1 (en) * 1992-03-04 1993-09-09 Boehringer Ingelheim Int Analysing host cell shut=off caused by Picorna viruses - by comparing translation prods. of viral 2C gene from RNA with and without cap structure, used e.g. to identify inhibitors of viral 2A protease
EP0585983A2 (en) * 1992-07-03 1994-03-09 Q.B.I. Enterprises Limited Nucleotide vector comprising a translation inhibition factor gene, and IRES region and a desired gene
US5358856A (en) * 1988-01-14 1994-10-25 Whitehead Institute For Biomedical Research Gene expression in mammalian cells using a cap independent 5' noncoding region

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5358856A (en) * 1988-01-14 1994-10-25 Whitehead Institute For Biomedical Research Gene expression in mammalian cells using a cap independent 5' noncoding region
WO1990001550A1 (en) * 1988-07-29 1990-02-22 Zymogenetics, Inc. High efficiency translation of polycistronic messages in eucaryotic cells
DE4206769A1 (en) * 1992-03-04 1993-09-09 Boehringer Ingelheim Int Analysing host cell shut=off caused by Picorna viruses - by comparing translation prods. of viral 2C gene from RNA with and without cap structure, used e.g. to identify inhibitors of viral 2A protease
EP0585983A2 (en) * 1992-07-03 1994-03-09 Q.B.I. Enterprises Limited Nucleotide vector comprising a translation inhibition factor gene, and IRES region and a desired gene

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DAVIES M V ET AL: "THE EFFECT OF POLIOVIRUS PROTEINASE 2A-P-R-O EXPRESSION ON CELLULAR METABOLISM INHIBITION OF DNA REPLICATION RNA POLYMERASE II TRANSCRIPTION AND TRANSLATION.", J BIOL CHEM 266 (22). 1991. 14714-14720. CODEN: JBCHA3 ISSN: 0021-9258, 5 August 1991 (1991-08-05), XP002012162 *

Also Published As

Publication number Publication date
IL112872A0 (en) 1995-06-29
WO1996027676A2 (en) 1996-09-12
AU5179596A (en) 1996-09-23

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