WO1996027379A1 - Carbopeptoids and carbonucleotoids - Google Patents

Carbopeptoids and carbonucleotoids Download PDF

Info

Publication number
WO1996027379A1
WO1996027379A1 PCT/US1996/003227 US9603227W WO9627379A1 WO 1996027379 A1 WO1996027379 A1 WO 1996027379A1 US 9603227 W US9603227 W US 9603227W WO 9627379 A1 WO9627379 A1 WO 9627379A1
Authority
WO
WIPO (PCT)
Prior art keywords
equivalents
carbon
carbohydrate
anomeric
solution
Prior art date
Application number
PCT/US1996/003227
Other languages
French (fr)
Other versions
WO1996027379A9 (en
Inventor
Kyriacos C. Nicolaou
Original Assignee
The Scripps Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Scripps Research Institute filed Critical The Scripps Research Institute
Priority to EP96908737A priority Critical patent/EP0827406A1/en
Priority to AU51882/96A priority patent/AU717099B2/en
Priority to US08/913,035 priority patent/US6204376B1/en
Publication of WO1996027379A1 publication Critical patent/WO1996027379A1/en
Publication of WO1996027379A9 publication Critical patent/WO1996027379A9/en
Priority to US10/140,597 priority patent/US20030013870A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D407/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00
    • C07D407/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00 containing two hetero rings
    • C07D407/04—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00 containing two hetero rings directly linked by a ring-member-to-ring-member bond
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D309/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only ring hetero atom, not condensed with other rings
    • C07D309/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only ring hetero atom, not condensed with other rings having no double bonds between ring members or between ring members and non-ring members
    • C07D309/08—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only ring hetero atom, not condensed with other rings having no double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D309/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only ring hetero atom, not condensed with other rings
    • C07D309/16—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only ring hetero atom, not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member
    • C07D309/20—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only ring hetero atom, not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member with hydrogen atoms and substituted hydrocarbon radicals directly attached to ring carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D309/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only ring hetero atom, not condensed with other rings
    • C07D309/16—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only ring hetero atom, not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member
    • C07D309/20—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only ring hetero atom, not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member with hydrogen atoms and substituted hydrocarbon radicals directly attached to ring carbon atoms
    • C07D309/22—Radicals substituted by oxygen atoms
    • C07D309/24—Methylol radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/655—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms
    • C07F9/6552—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms the oxygen atom being part of a six-membered ring
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support

Definitions

  • the invention relates to oligosaccharides and libraries incorporating oligosaccharide. More
  • the invention relates to oligosaccharides and libraries of oligosaccharides which employ amide and/or phosphodiester linkages for joining adjacent carbohydrate subunits.
  • Carbohydrates are known to mediate many cellular recognition processes. Carbohydrates can serve directly as binding molecules and, in such instances, are
  • Dysfunctional mediation of cellular recognition processes can lead to disease states. If a cellular recognition process is mediated by an oligosaccharide, then an absence or excess of such oligosaccharide can lead to a dysfunctional mediation of such process.
  • the mediating oligosaccharide may be deficient or absent due to a deficiency of production or due to a high rate of catabolism. If rate of catabolism is excessive, then catabolically resistant analogs of the bioactive
  • oligosaccharide may be preferred as drug candidates as compared to the native bioactive oligosaccharide .
  • a library which includes analogs of known bioactive oligosaccharides .
  • Such a library may be usefully employed for screening drug candidates .
  • oligopeptoids are shown by calculation to have greater conf ormational freedom as compared to conventional oligopeptides . Accordingly, oligopeptoids are thought to have greater potential as pharmaceutically useful binding ligands as compared to conventional oligopeptides having close sequence homology to such oligopeptoids .
  • Von Roedern et al disclose a carbohydrate amino acid (Angew. Chem, Int . Ed. Engl . 1994 , 31 , 687-689 ) . Although von Roedern discloses that carbohydrate amino acids may be coupled to peptides , he does not disclose that they may also be polymerized so as to form
  • a first aspect of the invention involves the molecular design and chemical synthesis of a class of carbohydrates designated as carbopeptoids (CPD ' s ) .
  • Glycopeptoids are preferred carbopeptoids .
  • Carbopeptoids and glcopeptoids are oligosaccharides which employ peptide- like amide bonds for linking the various
  • the invention is directed to a oligomeric carbopeptoid or glycopeptoid compound having carbohydrate amino acid subunits (CA ' s ) or glycoside amino acid subunits (GA ' s ) coupled to one another via an amide linkage .
  • the amide linkage may be represented by the formula CA 1 - (CO-NH) -CA 2 .
  • the amide linkage (CO-NH) includes a carbonyl carbon and an amido nitrogen .
  • a first carbohydrate amino acid subunit CA 1 or glycoside amino acid subunit GA 1 has an anomeric carbon bonded to the carbonyl carbon of the amide linkage .
  • the anomeric carbon of the first carbohydrate amino acid subunit CA 1 forms a C-glycosidic bond with the carbonyl carbon of the amide linkage and maintains the carbohydrate in a closed ring configuration .
  • a second carbohydrate amino acid subunit CA 2 has a non-anomeric carbon bonded to the amido nitrogen of the amide linkage .
  • the second carbohydrate amino acid subunit CA 2 like the first amino acid subunit CA 1 , may include an anomeric carbon bonded to the
  • carbohydrate amino acid subunit CA 2 is a terminal subunit , then its anomeric carbon may form a hemiacetal , a hemiketal , or a glycoside .
  • the invention is also directed to a process for synthesizing the above oligomeric carbopeptoid or glycopeptoid compound .
  • the synthetic process involves the coupling of two or more carbohydrate amino acid subunits (CA ' s) or glycoside amino acid subunits (GA' s ) to one another by means of amide linkages .
  • the invention is also directed to libraries of oligomeric carbopeptoid or glycopeptoid compounds . Such libraries are employable for drug screening .
  • Each oligomeric carbopeptoid or glydopeptoid compound includes at least two carbohydrate amino acid subunits (CA ' s ) or glycoside amino acid subunits (GA' s ) coupled to one another via an amide linkage as indicated above .
  • the invention is also directed to an improved process for synthesizing the above library of oligomers .
  • the process employs an elongation step for coupling the subunits to one another to produce the oligomers . In the elongation step, two carbohydrate amino acid subunits (CA ' s ) or glycoside amino acid subunits (GA' s ) are coupled to one another via an amide linkage as indicated above .
  • the invention is also directed to chemical
  • a first chemical intermediate is a derived carbohydrate amino acid having an anomeric carbon and non-anomeric carbons .
  • the anomeric carbon is substituted with a carboxyl radical .
  • Each of the non-anomeric carbons is substituted with a radical selected from the group consisting of blocked hydroxyl , blocked amino ,
  • a second chemical intermediate is a derived carbohydrate amino acid similar to the first except that the non-anomeric carbons are substituted with a radical selected from the group consisting of blocked hydroxyl , blocked amino , unprotected amino , and hydrogen, with the proviso that at least one radical is an
  • unprotected amino and at least one radical is a blocked hydroxyl or amino .
  • a second aspect of the invention involves the molecular design and chemical synthesis of a class of carbohydrates designated as carbonucleotoids (CND ' s ) .
  • Carbonucleotoids are oligosaccharides which employ oligonucleotide- like phosphate bonds for linking the various carbohydrate subunits within an oligomer
  • Phosphate bond formation may be achieved by employing technology and instrumentation developed for oligonucleotide synthesis .
  • the phosphate bonds employed within carbonucleotoids are convenient linkages for coupling these units . The ease and high efficiency by which the oligonucleotide- like linkages can be
  • the disclosed methods are characterized by their versatility and practicality.
  • the methods may exploit conventional solid phase and automated synthesis
  • oligomeric carbonucleotoid molecule comprising carbohydrate C-glycoside subunits (CG's) coupled to one another via a phosphodiester linkage.
  • the phosphodiester linkage may be represented by the structure: CG 1 -C 1 '-(O-PO(OH)-O)-CG 2 .
  • the first carbohydrate C-glycoside subunit (CG 1 -C 1 ') has an anomeric carbon forming a C-glycosidic bond with a carbon C 1 '. In turn the carbon C 1 ' is bonded to the phosphodiester linkage.
  • the second carbohydrate C-glycoside subunit CG 2 has a non-anomeric carbon bonded to the phosphodiester linkage.
  • the invention is also directed a process for synthesizing the oligomeric carbonucleotoid molecule.
  • the process employs a coupling step wherein two or more carbohydrate C-glycoside subunits (CG's) are coupled by means of a phosphodiester linkage as indicated above.
  • the second aspect of the invention is also directed to libraries of oligomeric carbonucleotoid molecules.
  • the libraries are employable for drug screening.
  • Each oligomeric carbonucleotoid molecule including at least two carbohydrate C-glycoside subunits (CG's) coupled to one another by means of a phosphodiester linkage as indicated above.
  • the invention is also directed to an improved process for synthesizing a library of oligomers .
  • the process employs an elongation step wherein subunits are coupled to one another to produce the oligomers .
  • the improvement is directed to the use of phosphodiester linkage linkages for linking the C-glycoside subunits as indicated above .
  • the second aspect of the invention is also directed to derived carbohydrate C-glycosides having an anomeric carbon and non-anomeric carbons .
  • the anomeric carbon forms a C-glycosidic bond with carbon C 1 ' .
  • the carbon C 1 ' is bonded to an phosphoramidite .
  • Each of the non-anomeric carbons is substituted with a radical selected from the group consisting of blocked hydroxyl , dif ferentially protected hydroxyl , and hydrogen , with the proviso that at least one radical is a differentially protected hydroxyl .
  • An alternative derived carbohydrate C-glycoside is similar to the above except that each of the non-anomeric carbons is substituted with a radical selected from the group consisting of blocked hydroxyl , unprotected hydroxyl , and hydrogen , with the proviso that at least one radical is an unprotected hydroxyl and at least one radical is a blocked hydroxyl .
  • the carbopeptoids are oligomers having repeating carbohydrate subunits linked to one another by means of amide linkage units. More particularly, the carbonyl carbon of each amide linkage unit is bonded to the anomeric carbon of a carbohydrate subunit.
  • the amide nitrogen of the amide linkage unit is bonded to a non-anomeric carbon.
  • the retrosynthetic scheme suggests that the amide bond may be split and that the preferred starting materials are carbohydrate amino acids.
  • Carbonucleotoids are oligosaccharides in which carbohydrate C-glycoside subunits (CG's) are linked to one another by means of phosphodiester bonds. More particularly, the retrosynthetic scheme suggests that the phosphate group may be eliminated, yielding hydroxy la ted starting material.
  • Scheme 2 illustrates representative carbohydrate amino acid subunits (CA's) and carbohydrate C-glycoside subunits (CG's).
  • Preferred carbohydrate amino acid subunits (CA's) include the following:
  • D-glucose having an unprotected carboxyl at the anomeric C(1) position, an unprotected amino group at the C(6) position, and blocked hydroxyls at the C(2), C ⁇ 3), and C(4) positions;
  • D-mannose having an unprotected carboxyl at the anomeric C(1) position, an unprotected amino group at the C(6) position, and blocked hydroxyls at the C(2), C(3), and C(4) positions;
  • D-galactose having an unprotected carboxyl at the anomeric C(1) position, an unprotected amino group at the C(6) position, and blocked hydroxyls at the C(2), C(3), and C(4) positions;
  • N-acetyl-D-glucosamine having an unprotected carboxyl at the anomeric C(1) position, an
  • ⁇ -D-altrose having an unprotected carboxyl at the anomeric C(1) position, an unprotected amino group at the C(6) position, and blocked hydroxyls at the C(2), C(3), and C(4) positions;
  • ⁇ -D-gulose having an unprotected carboxyl at the anomeric C(1) position, an unprotected amino group at the C(6) position, and blocked hydroxyls at the C(2), C(3), and C(4) positions;
  • ⁇ -D-glucose having an unprotected O-glycosidic amino at the anomeric C(1) position, an unprotected carboxyl as the C(6) position, and blocked hydroxyls at the C(2), C(3), and C(4) positions;
  • D-mannose having an unprotected O-glycosidic amino at the anomeric C(1) position, an unprotected carboxyl as the C(6) position, and blocked hydroxyls at the C(2), C(3), and C(4) positions;
  • D-galactose having an unprotected O-glycosidic amino at the anomeric C(1) position, an unprotected carboxyl as the C(6) position, and blocked hydroxyls at the C(2), C(3), and C(4) positions;
  • N-acetyl-D-glucosamine having an unprotected O- glycosidic amino at the anomeric C(1) position, an unprotected carboxyl as the C(6) position, a blocked amino group at the C(2) position and blocked hydroxyls at the C(3) and C(4) positions;
  • D-ribose having an unprotected carboxyl at the anomeric C(1) position, an unprotected amino group at the C(5) position, and blocked hydroxyls at the C(2) and C(3) positions;
  • D-arabinose having an unprotected carboxyl at the anomeric C(1) position, an unprotected amino group at the C(5) position, and blocked hydroxyls at the C(2) and C(3) positions.
  • Preferred carbohydrate amino acid subunits include the following:
  • D-glucose having a C(1) C 1' -glycosidic carbon bonded to a phosphoramidite, an unprotected hydroxyl at the C(6) position and blocked hydroxyls at the C(2), C(3), and C(4) positions;
  • D-mannose having a C(1) C 1' -glycosidic carbon bonded to a phosphoramidite, an unprotected hydroxyl at the C(6) position and blocked hydroxyls at the C(2), C(3), and C(4) positions;
  • D-galactose having a C(1) C 1' -glycosidic carbon bonded to a phosphoramidite, an unprotected hydroxyl at the C(6) position and blocked hydroxyls at the C(2), C(3), and C(4) positions;
  • N-acetyl-D-glucosamine having a C(1) C 1' -glycosidic carbon bonded to a phosphoramidite, an unprotected hydroxyl at the C(6) position, a blocked amino at the C(2) position, and blocked hydroxyls at the C(3) and C(4) positions.
  • CA's protected carbohydrate amino acid subunits from N-acetyl-D-glucosamine, i.e. compound 62.
  • Scheme 5 summarizes the synthesis of hexamer 74, i.e glucose-glucosamine hetero carbopeptoid (CPD).
  • Scheme 6 illustrates the construction of suitably protected and activated C-glycoside subunits (CG's) corresponding to glucose.
  • Scheme 7 illustrates the construction of suitably protected and activated C-glycoside subunits (CG's) corresponding to glucosamine.
  • Scheme 8 summarizes the synthesis of hexamer 116, i.e. glucose-glucosamine hetero carbonucleotoid (CND).
  • a oligosaccharide carbopeptoid (CPD) library may be constructed by performing using a split synthesis method of oligomerization as illustrated in Scheme 500 for carbopeptoids and Scheme 550 for carbonucleotoids .
  • the split synthesis may employ beads upon which to build the oligomers . Beads are aliguoted into each of a several reaction vessels , each reacrtion vessel containing a different core molecule . The core molecules are then allowed to attach to the beads . The beads are washed, mixed with one another , and then re-aliquoted ( split ) into a second set of reaction vessels for addition of a second core
  • oligosaccharides may then be screened using conventional methods developed for oligopeptide and oligonucleotide libraries . Screening an oligosaccharide library can lead to the identification of individual oligosaccharide components within the library having binding activity and/or bioactivity .
  • oligosaccharide libraries may be enlarged by introducing additional functionalities into the basic CA ' s and CG ' s .
  • oligosaccharide libraries may be further enlarged by enlarging the pool of free functional groups on the CA ' s and CG ' s and employed this enlarged pools of CA ' s and CG ' s during the respective split synthesis processes .
  • Scheme 20 illustrate a protocol published by Fuchs, E.F. et al. (J. Chem Ber. 1975, 108, 2254) for the synthesis of CA 45 and 46 from glucose pentaacetate.
  • Scheme 20 illustrates a synthetic route for CG 82, also starting from glucose pentaacetate.
  • the reagents and conditions for synthesizing CG 82 are provided as follows:
  • Step (g) (NCCH 2 CH 2 ) (NiPr 2 )PCl, tetrazole, CH 2 Cl 2 .
  • Step M The reagents and conditions for synthesizing CA 46 from CA 45 are provided in Step M as follows:
  • Step (c) NaN 3 .
  • Step (c) 10% HCOOH m CH 2 Cl 2 , 0°C, 2 minutes, 100%.
  • Step (d) RuCl 3 , NalO 4, CH 3 CN, H 2 O, CCl 4 , 20oC, 10 minutes,
  • Step (e) (1) 1 equiv. TsCl, base;
  • Step (f) NaN 3 .
  • Step (j) (1) 1 equiv. PivCl, base;
  • Step (k) (1) oxidative Nef; (2) CH 2 N 2
  • Step 1 DCC, HOBT, Et 3 , DMF;
  • Step 2 Piperidine, DMF
  • the crude product 50 is next dissolved in ethanol (0.15 M) and then concentrated H 2 S O 4 (0.01 equivalents-catalytic) is added. The reaction mixture is heated to 85 °C for eight hours.
  • the crude product 126 is dissolved in 25% NaOH (0.5 M) and heated at reflux for 18 hours (vigorous reflux is necessary).
  • triol 178 (.0 equiv.) in CH2Cl2 (-5 M) at 0 °C. was added triethylamine (1.2 equiv.), 4-DMAP (.10 equiv.) and then TOSCl ( 1.1 equiv.). The reaction is stirred for 1 h and then is quenched with saturated ammonium chloride ( 1.5 mL), diluted with ethyl acetate (25 mL), washed with water (2X 5 mL). brine ( 1X 5 mL), back-extracted (2X), recombined, dried (MgSO 4 ) and evaporated.
  • triol 182 (.0 equiv.) in CH 2 Cl 2 (.5 M) at 0 °C
  • sodium-azide ( 1.2 equiv.) from Aldrich chemical company at 0 °C.
  • the reaction is stirred for 1 h and then is quenched with saturated ammonium chloride ( 1 .5 mL), diluted with ethyl acetate (25 mL), washed with water (2X 5 mL), brine ( 1X 5 mL), back-extracted (2X), recombined, dried (MgSO 4 ) and evaporated.
  • the compound is purified by flash column chromatography and affords compound 183.
  • a solution of 201 ( 1.0 equivalents) is dissolved in ethanol (.01 M total) at 25 °C.
  • the mixture is next exposed to 10% Pd/C (.1 equivalents) and is then subsequently capped with a hydrogen balloon at 1 atmosphere.
  • the reaction is stirred for 72 hours and is then filtered through celite.
  • the crude mixture is subsequently diluted with ether and washed with NaHCO 3 (3X), brine ( 1 X) and dried (MgSO 4 ) and concentrated.
  • LAH lithiumaluminumhydride
  • a depiction of the generation of a combinatorial library for oligopeptoid compounds is shown in scheme 500.
  • the example uses an alphabet of eight D-aldose hexose sugars (other sugars groups such as the D/L ketoses and L-configurations of aldose hexoses, may be used) and carries the synthesis to a degree of three or 512 compounds. (The process can repeat itself to afford the library of desired size). Standard chemistry is shown and follows the reaction conditions as described above herein for peptoid synthesis.
  • the solid support used is the standard N-(2-Aminoethyl)-3-amino-propyl glass support; amino-polystyrene resin; aminopropyl glass; isothiocyanato glass and others as purchased from Sigma company. All supports may be with or without a linker extending from the amino group on the support
  • a depiction of the generation of a combinatorial library for oligonucleotoid compounds is shown in scheme 550.
  • the example uses an alphabet of eight D-aldose hexose sugars (other sugars groups such as the D/L ketoses and L-configurations of aldose hexoses. may be used) and carries the synthesis to a degree of three or 512 compounds. (The process can repeat itself to afford the library of desired size). Standard chemistry is shown and follows the reaction conditions as described above herein for carbonucleotoid synthesis.
  • the solid support used is the standard N-( 2-Aminoethyl)-3-amino-propyl glass support; amino-polystyrenc resin; aminopropyl glass; isothiocyanato glass and others as purchased from Sigma company. All supports may be with or without a linker extending from the amino group on the support (eg. succinate linkage, amide, ether, alkyl chain with terminal carbon activated as free alcohol, bromide etc.).
  • a linker extending from the amino group on the support (eg. succinate linkage, amide, ether, alkyl chain with terminal carbon activated as free alcohol, bromide etc.).
  • the benzylidene is then azeotroped with benzene (2X 100 mL) and then dried overnight under vacuum over P 2 O 5 .
  • a mixture of benzylidene, dibutyl tin oxide ( 1.2 equiv.) and dry methanol (.25 M) are heated at reflux for 4 h until the solution became clear and homogeneous. (An automatic stirring apparatus may be necessary.)
  • the solvent is next removed in vacuo to give a foamy white tin complex which was then azeotroped with benzene (2X) and dried (2 h to overnight) under vacuum over P2O5.
  • the solid support used is the standard N-(2-Aminoethyl)-3-amino-propyl glass support; amino-polystyrene resin; aminopropyl glass; isothiocyanato glass and others as purchased from Sigma company. All supports may be with or without a linker extending from the amino group on the support (eg. succinate linkage, amide, ether, alkyl chain with terminal carbon activated as free alcohol, bromide etc.).
  • a linker extending from the amino group on the support (eg. succinate linkage, amide, ether, alkyl chain with terminal carbon activated as free alcohol, bromide etc.).
  • TBDPS ether is then azeotroped with benzene (2X 100 mL) and then dried overnight under vacuum over P 2 O 5 .
  • the solid support used is the standard N-(2-Aminoethyl)-3-amino-propyl glass support; amino-polystyrene resin; aminopropyl glass; isothiocyanato glass and others as purchased from Sigma company. All supports may be with or without a linker extending from the amino group on the support (eg. succinate linkage, amide, ether, alkyl chain with terminal carbon activated as free alcohol, bromide etc.).
  • a linker extending from the amino group on the support (eg. succinate linkage, amide, ether, alkyl chain with terminal carbon activated as free alcohol, bromide etc.).
  • Aqueous layer is back extracted with ethyl acetate (3X) and then recombined with the organic layer which was then dried over MgSO 4 and evaporated. Purification by flash column chromatography yields the desired benzyl ether 2130.
  • the compound 2140 is then treated with tetrabutylammonium fluoride (2.0 equivalents) in THF (.1 Molar) and allowed to stir for an additional 2 hours at 25 °C.
  • a saturated solution of ammonium chloride (50 mL) is then added dropwise to quench the reaction mixture at 0 °C and the mixture was diluted with ethyl acetate, washed with water (2X), brine ( 1X), dried over M g S O 4 and evaporated. Purification by flash column chromatography yields tribenzyl ether 2150.
  • N- (2-Aminoethyl)-3-amino-propyl glass support amino-polystyrene resin; aminopropyl glass; isothiocyanato glass and others as purchased from Sigma company.
  • All supports may be with or without a linker extending from the amino group on the support (eg. succinate linkage, amide, ether, alkyl chain with terminal carbon activated as free alcohol, bromide etc.).
  • the concentrate is allowed to cool to room temperature and the product crystallizes overnight and carried on as follows:
  • the methyl glycoside is dissolved in chloroform (.5 M) and to it, is added phthalic anhydride ( 1.5 equiv.) and the reaction mixture is allowed to reflux at 70 °C for 4 h.
  • phthalic anhydride 1.5 equiv.
  • Phosphoramidate 138 (2 diastereomers): IR, (neat) cm -1 ; 3089, 2964, 2927, 2856, 2253, 1497, 1455, 1396, 1363, 1253, 1184, 1156, 1094, 1028, 978, 876, 836, 779, 735,

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Structural Engineering (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)

Abstract

Librairies are synthesized with oligomeric carbopeptoids and carbonucleotoids. Carbopeptoids are oligosaccharides having carbohydrate subunits linked to one another by amide bonds. Carbonucleotoids are oligosaccharides having carbohydrate subunits linked to one another by phosphodiester bonds. Carbopeptoid librairies may be fabricated using automated polypeptide synthesizers. Carbonucleotoid librairies may be fabricated using automated polynucleotide synthesizers.

Description

CARBOPEPTOIDS AND CARBONUCLEOTOIDS
Specification Field of the Invention:
The invention relates to oligosaccharides and libraries incorporating oligosaccharide. More
particularly, the invention relates to oligosaccharides and libraries of oligosaccharides which employ amide and/or phosphodiester linkages for joining adjacent carbohydrate subunits.
Background:
Carbohydrates are known to mediate many cellular recognition processes. Carbohydrates can serve directly as binding molecules and, in such instances, are
essential to the recognition process. A review of the biological role of carbohydrates with respect to cellular recognition phenomena is provided by Sharon et al.
(Scientific American, January 1993, 82). The emerging importance of glycobiology is further characterized by Mekelburger et al. (Angew. Chem. Int. Ed. Engl. 1992, 31, 1571) and by Dagani et al. (Chem. Eng. News, February 1, 1993, 28).
Dysfunctional mediation of cellular recognition processes can lead to disease states. If a cellular recognition process is mediated by an oligosaccharide, then an absence or excess of such oligosaccharide can lead to a dysfunctional mediation of such process. The mediating oligosaccharide may be deficient or absent due to a deficiency of production or due to a high rate of catabolism. If rate of catabolism is excessive, then catabolically resistant analogs of the bioactive
oligosaccharide may be preferred as drug candidates as compared to the native bioactive oligosaccharide .
Accordingly, what is needed is a library which includes analogs of known bioactive oligosaccharides . Such a library may be usefully employed for screening drug candidates .
Central requirements for the design of libraries of oligosaccharide analogs include the following :
(a) A need to maximize the potential of the designed oligosaccharides as ligand and drug candidates ;
(b) A need to capitalize on existing highly sophisticated technology directed to the synthesis of oligopeptides and oligonucleotides in order to facilitate the rapid and efficient design and construction of oligosaccharides ; and
(c ) A need for f lexibility with respect to
synthesizing either single target molecules or large libraries of target molecules simultaneously .
Methodologies for synthesizing biopolymers are well developed for peptides , nucleic acids , and saccharides .
Segments of oligopeptides and of oligonucleotides can now be routinely synthesized both in solution and in the solid phase, manually and/or on automated systems . The synthesis of such structures is facilitated by the availability of efficient techniques and sophisticated instrumentation for synthesizing peptide and phosphate bonds with high yields . The synthesis of oligopeptides and oligonucleotides is also facilitated by the absence of stereocenters in these linkages . In contrast , technology for the construction of oligosaccharides is comparatively less sophisticated and efficient .
Synthetic methods for constructing oligosaccharides give comparatively lower yields and are complicated by the two isomer possibilities (α and β) in glycoside bond
formation .
Techniques and chemical methods for simultaneously synthesizing multiple oligopeptides , e . g . 100-150
completely different peptides having lengths of up to 20 amino acid residues , are reviewed by Jung , G . et al .
(Angew. Chem, Int . Ed. Engl . 1992 , 31 , 367-383 - incorporated therein by reference) . Such techniques facilitate the construction of oligopeptide libraries .
Simon, et al . ( Proc, Natl . Acad. Sci . USA, 1992 , 89, 9367-9371 ) disclose oligopeptide analogs in which amino acid side chain groups are attached not to conventional peptide backbone carbons but to peptide backbone
nitrogens . Such analogs are termed peptoids . Simon also discloses the construction of peptoid libraries as a modular approach to drug discovery . Simon ' s
oligopeptoids are shown by calculation to have greater conf ormational freedom as compared to conventional oligopeptides . Accordingly, oligopeptoids are thought to have greater potential as pharmaceutically useful binding ligands as compared to conventional oligopeptides having close sequence homology to such oligopeptoids .
Von Roedern et al . disclose a carbohydrate amino acid (Angew. Chem, Int . Ed. Engl . 1994 , 31 , 687-689 ) . Although von Roedern discloses that carbohydrate amino acids may be coupled to peptides , he does not disclose that they may also be polymerized so as to form
oligosaccharides .
Summary :
A first aspect of the invention involves the molecular design and chemical synthesis of a class of carbohydrates designated as carbopeptoids (CPD ' s ) .
Glycopeptoids are preferred carbopeptoids . Carbopeptoids and glcopeptoids are oligosaccharides which employ peptide- like amide bonds for linking the various
carbohydrate subunits within an oligomer assembly . Amide bond formation may be achieved by employing oligopeptide synthesis technology and instrumentation . The method allows for the design and synthesis of specific compounds for biological and pharmacological investigations . The method also allows for the generation of libraries of compounds for biological and pharmacological screening . Conventional screening techniques employed with respect to peptide and peptoid libraries ( Simon et al . , supra) may also be employed with respect to carbopeptoid libraries . The design takes advantage of the
multifunctionality of carbohydrate subunits to maximize the binding properties of the molecules . The ease and high efficiency by which the peptide-like linkages can be constructed make the synthesis of these molecules a practical proposition . Furthermore , non-carbohydrate units may be inserted into the sequence making this approach even more flexible and versatile for the generation of new libraries of organic compounds .
More particularly, the invention is directed to a oligomeric carbopeptoid or glycopeptoid compound having carbohydrate amino acid subunits (CA ' s ) or glycoside amino acid subunits (GA ' s ) coupled to one another via an amide linkage . The amide linkage may be represented by the formula CA1 - (CO-NH) -CA2 . The amide linkage (CO-NH) includes a carbonyl carbon and an amido nitrogen . A first carbohydrate amino acid subunit CA1 or glycoside amino acid subunit GA1 has an anomeric carbon bonded to the carbonyl carbon of the amide linkage . The anomeric carbon of the first carbohydrate amino acid subunit CA1 forms a C-glycosidic bond with the carbonyl carbon of the amide linkage and maintains the carbohydrate in a closed ring configuration . A second carbohydrate amino acid subunit CA2 has a non-anomeric carbon bonded to the amido nitrogen of the amide linkage . The second carbohydrate amino acid subunit CA2 , like the first amino acid subunit CA1 , may include an anomeric carbon bonded to the
carbonyl carbon of a second amide linkage linking the second carbohydrate amino acid subunit CA2 to a third carbohydrate amino acid subunit CA3 , etc . In this instance , the anomeric carbon of the second carbohydrate amino acid subunit CA2 forms a C-glycosidic bond with the carbonyl carbon of the amide linkage and maintains the carbohydrate in a closed ring
configuration . On the other hand, if the second
carbohydrate amino acid subunit CA2 is a terminal subunit , then its anomeric carbon may form a hemiacetal , a hemiketal , or a glycoside .
The invention is also directed to a process for synthesizing the above oligomeric carbopeptoid or glycopeptoid compound . The synthetic process involves the coupling of two or more carbohydrate amino acid subunits (CA ' s) or glycoside amino acid subunits (GA' s ) to one another by means of amide linkages .
The invention is also directed to libraries of oligomeric carbopeptoid or glycopeptoid compounds . Such libraries are employable for drug screening . Each oligomeric carbopeptoid or glydopeptoid compound includes at least two carbohydrate amino acid subunits (CA ' s ) or glycoside amino acid subunits (GA' s ) coupled to one another via an amide linkage as indicated above . The invention is also directed to an improved process for synthesizing the above library of oligomers . The process employs an elongation step for coupling the subunits to one another to produce the oligomers . In the elongation step, two carbohydrate amino acid subunits (CA ' s ) or glycoside amino acid subunits (GA' s ) are coupled to one another via an amide linkage as indicated above .
The invention is also directed to chemical
intermediates for producing oligomeric carbopeptoids . A first chemical intermediate is a derived carbohydrate amino acid having an anomeric carbon and non-anomeric carbons . The anomeric carbon is substituted with a carboxyl radical . Each of the non-anomeric carbons is substituted with a radical selected from the group consisting of blocked hydroxyl , blocked amino ,
differentially protected amino , and hydrogen, with the proviso that at least one radical is a differentially protected amino . A second chemical intermediate is a derived carbohydrate amino acid similar to the first except that the non-anomeric carbons are substituted with a radical selected from the group consisting of blocked hydroxyl , blocked amino , unprotected amino , and hydrogen, with the proviso that at least one radical is an
unprotected amino and at least one radical is a blocked hydroxyl or amino .
A second aspect of the invention involves the molecular design and chemical synthesis of a class of carbohydrates designated as carbonucleotoids (CND ' s ) . Carbonucleotoids are oligosaccharides which employ oligonucleotide- like phosphate bonds for linking the various carbohydrate subunits within an oligomer
assembly . Phosphate bond formation may be achieved by employing technology and instrumentation developed for oligonucleotide synthesis . The phosphate bonds employed within carbonucleotoids are convenient linkages for coupling these units . The ease and high efficiency by which the oligonucleotide- like linkages can be
constructed make the synthesis of these molecules a practical proposition.
The disclosed methods are characterized by their versatility and practicality. The methods may exploit conventional solid phase and automated synthesis
techniques for producing carbopeptoids and
carbonucleotoids in large scale.
More particularly, the second aspect of the
invention is directed to an oligomeric carbonucleotoid molecule comprising carbohydrate C-glycoside subunits (CG's) coupled to one another via a phosphodiester linkage. The phosphodiester linkage may be represented by the structure: CG1-C1'-(O-PO(OH)-O)-CG2. The first carbohydrate C-glycoside subunit (CG1-C1') has an anomeric carbon forming a C-glycosidic bond with a carbon C1'. In turn the carbon C1' is bonded to the phosphodiester linkage. The second carbohydrate C-glycoside subunit CG2 has a non-anomeric carbon bonded to the phosphodiester linkage. The invention is also directed a process for synthesizing the oligomeric carbonucleotoid molecule. The process employs a coupling step wherein two or more carbohydrate C-glycoside subunits (CG's) are coupled by means of a phosphodiester linkage as indicated above.
The second aspect of the invention is also directed to libraries of oligomeric carbonucleotoid molecules. The libraries are employable for drug screening. Each oligomeric carbonucleotoid molecule including at least two carbohydrate C-glycoside subunits (CG's) coupled to one another by means of a phosphodiester linkage as indicated above. The invention is also directed to an improved process for synthesizing a library of oligomers . The process employs an elongation step wherein subunits are coupled to one another to produce the oligomers . The improvement is directed to the use of phosphodiester linkage linkages for linking the C-glycoside subunits as indicated above .
The second aspect of the invention is also directed to derived carbohydrate C-glycosides having an anomeric carbon and non-anomeric carbons . The anomeric carbon forms a C-glycosidic bond with carbon C1 ' . In turn, the carbon C1 ' is bonded to an phosphoramidite . Each of the non-anomeric carbons is substituted with a radical selected from the group consisting of blocked hydroxyl , dif ferentially protected hydroxyl , and hydrogen , with the proviso that at least one radical is a differentially protected hydroxyl . An alternative derived carbohydrate C-glycoside is similar to the above except that each of the non-anomeric carbons is substituted with a radical selected from the group consisting of blocked hydroxyl , unprotected hydroxyl , and hydrogen , with the proviso that at least one radical is an unprotected hydroxyl and at least one radical is a blocked hydroxyl .
Figure imgf000012_0001
Detailed Description:
Retrosynthetic schemes for carbopeptoids (compound I) and carbonucleotoids (compound II) are illustrated in Scheme 1.
The carbopeptoids (CPD's) are oligomers having repeating carbohydrate subunits linked to one another by means of amide linkage units. More particularly, the carbonyl carbon of each amide linkage unit is bonded to the anomeric carbon of a carbohydrate subunit.
Similarly, the amide nitrogen of the amide linkage unit is bonded to a non-anomeric carbon. The retrosynthetic scheme suggests that the amide bond may be split and that the preferred starting materials are carbohydrate amino acids.
Carbonucleotoids (CND's) are oligosaccharides in which carbohydrate C-glycoside subunits (CG's) are linked to one another by means of phosphodiester bonds. More particularly, the retrosynthetic scheme suggests that the phosphate group may be eliminated, yielding hydroxy la ted starting material.
Scheme 2 illustrates representative carbohydrate amino acid subunits (CA's) and carbohydrate C-glycoside subunits (CG's). Preferred carbohydrate amino acid subunits (CA's) include the following:
D-glucose having an unprotected carboxyl at the anomeric C(1) position, an unprotected amino group at the C(6) position, and blocked hydroxyls at the C(2), C{3), and C(4) positions;
D-mannose having an unprotected carboxyl at the anomeric C(1) position, an unprotected amino group at the C(6) position, and blocked hydroxyls at the C(2), C(3), and C(4) positions;
D-galactose having an unprotected carboxyl at the anomeric C(1) position, an unprotected amino group at the C(6) position, and blocked hydroxyls at the C(2), C(3), and C(4) positions;
Figure imgf000015_0001
N-acetyl-D-glucosamine having an unprotected carboxyl at the anomeric C(1) position, an
unprotected amino group at the C(6) position, a blocked amino group at the C(2) position, and blocked hydroxyls at the C(3) and C(4) positions; α-D-idose having an unprotected carboxyl at the anomeric C(1) position, an unprotected amino group at the C(6) position, and blocked hydroxyls at the C(2), C(3), and C(4) positions;
α-D-altrose having an unprotected carboxyl at the anomeric C(1) position, an unprotected amino group at the C(6) position, and blocked hydroxyls at the C(2), C(3), and C(4) positions;
α-D-gulose having an unprotected carboxyl at the anomeric C(1) position, an unprotected amino group at the C(6) position, and blocked hydroxyls at the C(2), C(3), and C(4) positions;
α-D-glucose having an unprotected O-glycosidic amino at the anomeric C(1) position, an unprotected carboxyl as the C(6) position, and blocked hydroxyls at the C(2), C(3), and C(4) positions;
D-mannose having an unprotected O-glycosidic amino at the anomeric C(1) position, an unprotected carboxyl as the C(6) position, and blocked hydroxyls at the C(2), C(3), and C(4) positions;
D-galactose having an unprotected O-glycosidic amino at the anomeric C(1) position, an unprotected carboxyl as the C(6) position, and blocked hydroxyls at the C(2), C(3), and C(4) positions; N-acetyl-D-glucosamine having an unprotected O- glycosidic amino at the anomeric C(1) position, an unprotected carboxyl as the C(6) position, a blocked amino group at the C(2) position and blocked hydroxyls at the C(3) and C(4) positions;
D-ribose having an unprotected carboxyl at the anomeric C(1) position, an unprotected amino group at the C(5) position, and blocked hydroxyls at the C(2) and C(3) positions; and
D-arabinose having an unprotected carboxyl at the anomeric C(1) position, an unprotected amino group at the C(5) position, and blocked hydroxyls at the C(2) and C(3) positions. Preferred carbohydrate amino acid subunits (CA's) include the following:
D-glucose having a C(1) C1'-glycosidic carbon bonded to a phosphoramidite, an unprotected hydroxyl at the C(6) position and blocked hydroxyls at the C(2), C(3), and C(4) positions;
D-mannose having a C(1) C1'-glycosidic carbon bonded to a phosphoramidite, an unprotected hydroxyl at the C(6) position and blocked hydroxyls at the C(2), C(3), and C(4) positions;
D-galactose having a C(1) C1'-glycosidic carbon bonded to a phosphoramidite, an unprotected hydroxyl at the C(6) position and blocked hydroxyls at the C(2), C(3), and C(4) positions; and
N-acetyl-D-glucosamine having a C(1) C1'-glycosidic carbon bonded to a phosphoramidite, an unprotected hydroxyl at the C(6) position, a blocked amino at the C(2) position, and blocked hydroxyls at the C(3) and C(4) positions.
Scheme 3 outlines a preferred synthesis of suitably protected carbohydrate amino acid subunits (CA's) from D-glucose, i.e. compound 46.
Figure imgf000019_0001
Scheme 4 outlines the synthesis of suitably
protected carbohydrate amino acid subunits (CA's) from N-acetyl-D-glucosamine, i.e. compound 62.
Figure imgf000021_0001
Scheme 5 summarizes the synthesis of hexamer 74, i.e glucose-glucosamine hetero carbopeptoid (CPD).
Figure imgf000023_0001
Figure imgf000024_0001
Scheme 6 illustrates the construction of suitably protected and activated C-glycoside subunits (CG's) corresponding to glucose.
Figure imgf000026_0001
Scheme 7 illustrates the construction of suitably protected and activated C-glycoside subunits (CG's) corresponding to glucosamine.
Figure imgf000028_0001
Scheme 8 summarizes the synthesis of hexamer 116, i.e. glucose-glucosamine hetero carbonucleotoid (CND).
Figure imgf000029_0001
Figure imgf000030_0001
The chemistries illustrated in Schemes 5 and 8 for synthesizing heterohexamer CPD 74 and heterohexamer CND 116 can also be employed for synthesizing homohexamer CPD's 118 (glucose) and 120 (glucosamine) and homohexamer CND's 122 (glucose) and 124 (glucosamine).
Figure imgf000032_0001
In analogy with the construction of oligopeptide and oligonucleotide libraries , a oligosaccharide carbopeptoid (CPD) library may be constructed by performing using a split synthesis method of oligomerization as illustrated in Scheme 500 for carbopeptoids and Scheme 550 for carbonucleotoids . For example, the split synthesis may employ beads upon which to build the oligomers . Beads are aliguoted into each of a several reaction vessels , each reacrtion vessel containing a different core molecule . The core molecules are then allowed to attach to the beads . The beads are washed, mixed with one another , and then re-aliquoted ( split ) into a second set of reaction vessels for addition of a second core
molecule to the first added core molecule . The process is then reiterated until the oligomerization process is complete . The resultant library of oligosaccharides may then be screened using conventional methods developed for oligopeptide and oligonucleotide libraries . Screening an oligosaccharide library can lead to the identification of individual oligosaccharide components within the library having binding activity and/or bioactivity .
The above oligosaccharide libraries (CPD and CND) may be enlarged by introducing additional functionalities into the basic CA ' s and CG ' s .
The above oligosaccharide libraries (CPD and CND) may be further enlarged by enlarging the pool of free functional groups on the CA ' s and CG ' s and employed this enlarged pools of CA ' s and CG ' s during the respective split synthesis processes . Scheme 20 illustrate a protocol published by Fuchs, E.F. et al. (J. Chem Ber. 1975, 108, 2254) for the synthesis of CA 45 and 46 from glucose pentaacetate.
Additionally, Scheme 20 illustrates a synthetic route for CG 82, also starting from glucose pentaacetate. The reagents and conditions for synthesizing CG 82 are provided as follows:
Steps (a)-(d): according to Fuchs (supra). Step (e): (1) DMTCl, DMAP, Pyridine; room temperature.
(2) TESTfl; 0°C.
Step (f): DIBAL-H, CH2Cl2 ; -78°C; and
Step (g) : (NCCH2CH2) (NiPr2)PCl, tetrazole, CH2Cl2.
The reagents and conditions for synthesizing CA 46 from CA 45 are provided in Step M as follows:
Step (m): FMOC-Cl, K2CO3, THF, H2O; 0°C .
Figure imgf000035_0001
A synthetic route for producing C-glycosides (CG's) with β-configuration at the former anomer center is illustrated in Scheme 21. The starting material
(compound 36) is commercially available. The reagents and conditions for synthesizing CG 181 and CG 185 are as follows:
Step (a): Co2(CO)8, HSiEt2Me, CO.
Step (b): (1) AcOH, H2O, THF;
(2) RuCl3, NalO4, CH3CN, H2O, CCL4, room temperature;
Step (c): NaOMe, MeOH;
Step (d): (1) DMTCl, DMAP, Pyridine, room temperature;
(2) TESOTf;
Step (e): BH3-THF;
Step (f): (NCCH2CH2) (NiPr2)PCl, tetrazole, Ch2Cl2;
Step (g): (1) 1 equiv TsCl. base;
(2) TESOTf;
Step (h): NaN3;
Step (i): H2, Pd(OH)2-C;
Step (j): FMOC-Cl, base.
Figure imgf000037_0001
Figure imgf000038_0001
Synthetic routes for producing with C-glycosides with α-configurations at the former anomeric center, i.e. CG 196 and CG 1204, are illustrated in Scheme 22. The common starting material for these synthetic routes
(compound 190) is disclosed by Schmidt, R. R. et al.
(Liebigs Ann. Chem. 1987, 825). The reagents and
conditions for the reactions leading to CG 196 and CG 204 are as follows:
Step (a): reductive debenzylation;
Step (b): (1) equiv TsCl. base;
( 2 ) TESOTf .
Step (c) : NaN3.
Step (d): RuCl3, NalO4, CH3CN, H2O, CCl4.
Step (e): H2 , Pd-C .
Step (f): FMOC-Cl, base.
Step (g): (1) DMTCl, DMAP, Pyridine, room
temperature;
(2) TESOTf .
Step (h): (1) RuCl3, NalO4, CH3CN, H2O, CCl4;
(2) CH2N2.
Step (i): DIBAL-H.
Step (j): PPh3, DIAD, diphenyl phosphoryl azide (DPPA), THF.
Step (k): KMnO4, t-BuOH, buffer.
Reactions for the development of the galactose derived C-glycoside 138 into protected CA's and diols is illustrate in Scheme 23. The common starting material for these synthetic routes (compound 138) is disclosed by Petrus, L. et al. (Chem. zvesti. 1982, 36, 103). The reagents and conditions required for the synthesis of compound 209, compound 214, compound 220, and compound 224 are indicated below:
Step (a): (1) 1.1 equivalent DMTCl, DMAP, Pyridine, 12 hour, 20°C;
(2) TesOTf, CH2, 0°C, 1 hour, 83%.
Step (b): (1) LAH, ether, reflux, 2 hour;
(2) FMOC-Cl, K2CO3, THF, H2O, 0°C, 1 hour, 55%;
Step (c) 10% HCOOH m CH2Cl2 , 0°C, 2 minutes, 100%.
Step (d) RuCl3, NalO4, CH3CN, H2O, CCl4, 20ºC, 10 minutes,
54%.
Step (e) (1) 1 equiv. TsCl, base;
(2) TESOTf.
Step (f) NaN3.
Step (g) oxidative NEF.
Step (h) Pd-C, H2.
Step (i) FMOC-Cl, base.
Step (j) (1) 1 equiv. PivCl, base;
(2) TESOTf.
Step (k) (1) oxidative Nef; (2) CH2N2
Step (l) DIBAL-H.
Step (m) DMTCl, DMAP, Pyridine.
Step (n) LAH.
Step (o) Nef reaction
Step (p) LAH.
Figure imgf000041_0001
An exemplary protocol for synthesizing a hexamer carbopeptoid (CPD 234) starting from galactose derived CA 214, glucosamine derived CA 62, and glucose derived CA, using standard methods for solid phase peptide synthesis is illustrated in Scheme 24. The reagents and condition for these reactions are as follows:
Step 1: DCC, HOBT, Et3, DMF;
Step 2 : Piperidine, DMF
Figure imgf000043_0001
Figure imgf000044_0001
THIS PAGE WAS MISSING UPON FILING
SYNTHETIC METHODS
Preparation of 37
Figure imgf000046_0001
To a solution of β -D-Glucose pentaacetate 36 i n nitromethane from Aldrich company ( .13 Molar), is added trimethylsilyl cyanide (3.0 equivalents) and then SnCl4 ( .02 equivalents). The mixture is stirred for one hour and then an aqueous solution of sodium acetate was added to hydrolyze the remaining trimethylsilyl cyanide. The mixture is evaporated and the remaining oil is resuspended in dichloromethane and washed with sodium acetate solution ( 1X), water ( 1X ). brine ( 1X) and then dried over magnesium sulphate and con centrated. The crude solid is then recrystallized from methanol to yield 37 as a white solid (47%). scheme 3 step 1; scheme 9, step a.
Preparation of 38
Figure imgf000047_0002
The crude product 37 is next dissolved in ethanol (0.15 M) and then concentrated H2S O 4 (0.01 equivalents-catalytic) is added. The reaction mixture is heated to 85 °C for eight hours. The solution is next concentrated in vacuo and purification by flash column chromatography affords compound 38. scheme 3 step 2
Preparation of 39
Figure imgf000047_0001
To a solution of 38 ( 1.0 equivalents) in pyridine ( .10 Molar), is added trimethylacetyl chloride (pivaloyl chloride) (2.5 equivalents) at 0 °C. The reaction is stirred for 2 hours and then diluted with diethylether and washed with ammonium chloride (2X), copper sulfate (2X), brine (IX), dried over MgSO4 and concentrated. Purification by flash column chromatography affords compound 39. scheme 3 step 1
Preparation of 40
Figure imgf000048_0001
To a solution of 39 (1.0 equivalents) in methylene chloride
(.10 Molar), is added diisopropylethylamine (3.3 equivalents) at 0 °C. Subsequent addition of triethylsily 1 trifluoromethanesulfonate (3.3 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X), brine
(IX) and then dried (MgSO4) and concentrated. Purification by flash column chromatography affords compound 40. scheme 3 step 2
Preparation of 41
Figure imgf000049_0002
To a solution of 40 in ethanol (.13 Molar), is added sodium ethoxide (0.3 equivalents) and the reaction mixture is stirred f or two hours at room temperature. The solution is then concentrated in vacuo and purification by flash column chromatography affords compound 41. scheme 3 step 1
Preparation of 42
Figure imgf000049_0001
A solution of 41 ( 1.0 equivalents) in tetrahydrofuran (.18
M) is treated with DPPA (diphenylphosphorylazide, 2.0 equivalents), triphenylphosphine ( 1.3 equivalents) and DIAD (diisopropyl-azo-dicarboxylate, 1.3 equivalents). The reaction is heated to 80 °C for 3 hours and then diluted with ether (2X) and washed with .5 M aqueous NaOH (2X). The organic layer is dried over MgSO4 and evaporated. Purification by flash column chromatography affords compound 42. scheme 3 step 2
Preparation of 44
Figure imgf000050_0001
A solution of 42 ( 1.0 equivalents) is dissolved in ethanol
(.01 M total) at 25 °C. The mixture is next exposed to 10% Pd/C ( .1 equivalents) and is then subsequently capped with a hydrogen balloon at 1 atmosphere. The reaction is stirred for 72 hours and is then filtered through celite. The crude mixture is subsequently diluted with ether and washed with NaHCO3 (3X), brine ( I X) and dried (MgSO4) and concentrated. Purification by flash column chromatography affords compound 44. scheme 3 step 1
Preparation of 45
Figure imgf000051_0001
A solution of 44 ( 1.0 equivalents) is dissolved in p-dioxanes (.1 M) and then exposed to a solution 3.0 Molar solution of sodium hydroxide ( 1.5 equivalents). The reaction is then stirred for 2 hours at 50 °C and is subsequently diluted with ether and washed with a solution of NH4CI (3X), brine ( IX) and dried (MgSO4 ) and concentrated. Purification by flash column chromatography affords compound 45. scheme 3 step 1
Preparation of 46
To a solution of 45 ( 1.0 equivalents) in methylene chloride (.10 Molar), is added sodium bicarbonate (2.0 equivalents) at 0 °C. Subsequent addition of 9-fluorenylmethyl chloroformate (FMOC-Cl, 1.2 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X), brine ( I X) and then dried ( M g S O 4 ) and concentrated. Purification by flash column chromatography affords compound 46. scheme 3 step 2
Preparation of 48
Figure imgf000052_0001
Procedure as described in Methods in Carbohydrate chemistry, Whistler, R., II, 1963 , p. 327. A mixture of 80g anhydrous D-glucosamine hydrochloride or D-galactosamine hydrochloride from Aldrich chemical company, in 200 mL. methanol and 20g Dowex 50 (H+) acidic resin, is stirred at the boiling point in a round bottom flask. After 24-hr. reaction time, the resin is removed by filtration and ished three times with 20 ml. of methanol. The filrate and ishings are combined and concentrated to about 125 ml by rotovap. The concentrate is allowed to cool to room temperature and the product crystallizes overnight.
To a solution of free amine, in chloroform (.5 M), is added phthalic anhydride ( 1.5 equiv.) and the reaction mixture is allowed to reflux at 70 °C for 4 h. The product is then crystallized and carried onto the next step.
To a solution of triol in methylene chloride (.5 M), is added acetic anhydride (3.5 equiv.) and triethyl amine (3.5 equiv.) and the reaction mixture is allowed to stir at 0 °C for 4 h. The product 48 , is then crystallized or purified by flash column chromatography and carried onto the next step.
Preparation of 50
Figure imgf000054_0001
To a solution of N-phthalamido-D-Glucosamine tetraacetae
48 in nitromethane (.13 Molar), is added trimethylsilyl cyanide (3.0 equivalents) and then SnCl 4 (.02 equivalents). The mixture is stirred for one hour and then an aqueous solution of sodium acetate was added to hydrolyze the remaining trimethylsilyl cyanide. The mixture is evaporated and the remaining oil is resuspended in dichloromethane and washed with sodium acetate solution ( I X), water ( IX), brine ( IX) and then dried over magnesium sulphate and concentrated. The crude solid is then recrystallized from methanol to yield 50 as a white solid (47%). scheme 4
Preparation of 52
The crude product 50 is next dissolved in ethanol (0.15 M) and then concentrated H2 S O 4 (0.01 equivalents-catalytic) is added. The reaction mixture is heated to 85 °C for eight hours.
The solution is next concentrated in vacuo and purification by flash column chromatography affords compound 52. scheme 4
Preparation of 54
Figure imgf000055_0002
A solution of 52 ( 1.0 equivalents) is dissolved in methanol (.1 M total). The reaction is then charged with acetic anhydride ( 1.1 equivalents) and is subsequently stirred for 2 hours at 30 °C. The reaction is next diluted with ether and washed with NaHCO 3 (3X), brine ( 1 X) and dried (MgSO4) and concentrated. Purification by flash column chromatography affords compound 54. scheme 4
Preparation of 55
Figure imgf000055_0001
To a solution of 54 (1.0 equivalents) in pyridine (.10 Molar), is added trimethylacetylchloride (pivaloyl chloride) (2.5 equivalents) at 0 °C. The reaction is stirred for 2 hours and then diluted with diethylether and washed with ammonium chloride (2X), copper sulfate (2X), brine (IX), dried over MgSO4 and concentrated. Purification by flash column chromatography affords compound 55. scheme 4
Preparation of 56
Figure imgf000056_0001
To a solution of 55 (1.0 equivalents) in methylene chloride (.10 Molar), is added diisopropylethylamine (2.2 equivalents) at 0 °C. Subsequent addition of trie thylsilyl trifluoromethanesulfonate (2.2 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X). brine (IX) and then dried (MgSO4) and concentrated. Purification by flash column chromatography affords compound 56. scheme 4 Preparation of 57
Figure imgf000057_0002
To a solution of 56 in ethanol (.13 Molar), is added sodium ethoxide (0.3 equivalents) and the reaction mixture is stirred for two hours at room temperature. The solution is then concentrated in vacuo and purification by flash column chromatography affords compound 57. scheme 4
Preparation of 58
Figure imgf000057_0001
A solution of 57 ( 1.0 equivalents) in tetrahydrofuran (.18 M) is treated with DPPA (diphenylphosphorylazide, 2.0 equivalents), triphenylphosphine ( 1 .3 equivalents) and DIAD (diisopropyl-azo-dicarboxylate, 1.3 equivalents). The reaction is heated to 80 °C for 3 hours and then diluted with ether (2X) and washed with .5 M aqueous NaOH (2X). The organic layer is dried over MgSO4 and evaporated. Purification by flash column chromatography affords compound 58. scheme 4
Preparation of 60
Figure imgf000058_0001
A solution of 58 ( 1.0 equivalents) is dissolved in ethanol (.01 M total) at 25 °C. The mixture is next exposed to 10% Pd/C (.1 equivalents) and is then subsequently capped with a hydrogen balloon at 1 atm. The reaction is stirred for 72 hours and is then filtered through celite. The crude mixture is subsequently diluted with ether and washed with NaHCO3 (3X), brine (IX) and dried (MgSO4) and concentrated. Purification by flash column chromatography affords compound 60. scheme 4
Preparation of 61
Figure imgf000059_0002
A solution of 60 ( 1.0 equivalents) is dissolved in p-dioxanes ( .1 M) and then exposed to a solution 3.0 Molar solution of sodium hydroxide ( 1.5 equivalents). The reaction is then stirred for 2 hours at 50 °C and is subsequently diluted with ether and washed with a solution of NH4CI (3X), brine ( IX) and dried (MgSO4 ) and concentrated. Purification by flash column chromatography affords compound 61. scheme 4
Preparation of 62
Figure imgf000059_0001
To a solution of 61 ( 1.0 equivalents) in methylene chloride (.10 Molar), is added sodium bicarbonate (2.0 equivalents) at 0 °C. Subsequent addition of 9-fluorenylmethyl chloroformate (FMOC-Cl, 1.2 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X), brine ( IX) and then dried ( M g S O 4 ) and concentrated. Purification by flash column chromatography affords compound 62. scheme 4
Preparation of 63
Figure imgf000060_0001
To a stirred solution of the acid 46 ( 1.0 equivalents) and the amine 60 ( 1. 1 equivalents) in dimethylformamide ( . 10 Molar) at 25 °C, is added 1 -hydroxybenzotriazole (HOBT; 1.1 equivalents). Next dicyclohexylcarbodiimide ( 1.2 equivalents) is added and the reaction is stirred for 14 hours. The mixture is diluted with ether, filtered and the filtrate is washed with aqueous NaHCO3 (2X), water (2X). and brine (2X). The organic phase is dried over MgSO4 and then concentrated. Purification by flash column chromatography affords compound 63 . scheme 5 step 1
Preparation of 64
Figure imgf000061_0002
To a stirred solution of 63 ( 1 .0 equivalents ) in dimethylformamide (.10 Molar) at 25 °C, is added piperidine ( 1.1 equivalents). The reaction is stirred for 1 hour and is then diluted with ether, and washed with aqueous CUSO4 (2X), water (2X), and brine (2X). The organic phase is dried over MgSO4 and then concentrated . Purification by flash column chromatography affords compound 64. scheme 5 step 2
Preparation of 65
Figure imgf000061_0001
To a stirred solution of the acid 62 ( 1.0 equivalents) and the amine 64 ( 1.1 equivalents) in dimethylformamide (.10 Molar) at 25 °C, is added 1 -hydroxybenzotriazole (HOBT; 1.1 equivalents). Note: numerous iterations can be performed using the acid 62 or intermixing with other acids including for example acid 46 to form successive oligomers where n=2 to infinity (a hexamer is shown in scheme 5 ) to obtain large carbopeptoid libraries. Next dicyclohexylcarbodiimide ( 1.2 equivalents) is added and the reaction is stirred for 14 hours. The mixture is diluted with ether, filtered and the filtrate is washed with aqueous NaHCO3 (2X), water (2X), and brine (2X). The organic phase is dried over MgSO4 and then concentrated. Purification by flash column chromatography affords compound 65. scheme 5 step 1
Preparation of 66
Figure imgf000062_0001
To stirred solution of 65 ( 1 .0 equivalents) i n dimethylformamide (.10 Molar) at 25 °C, is added piperidine ( 1.1 equivalents). The reaction is stirred for 1 hour and is then diluted with ether, and washed with aqueous CUSO4 (2X), water (2X), and brine (2X). The organic phase is dried over MgSO4 and then concentrated. Purification by flash column chromatography affords compound 66. Note: n um erous iterations can be performed using variable length oligomers of 66 to form peptoid oligomers where n=2 to infinity (a hexamer is shown in scheme 5). scheme 5 step 2
Preparation of 67
Figure imgf000063_0001
To a stirred solution of the acid 46 ( 1 .0 equivalents ) and the amine 66 ( 1 . 1 equivalents) in dimethylformamide ( .10 Molar) at 25 °C, is added 1 -hydroxybenzotriazole (HOBT; 1.1 equivalents). Note: numerous iterations can be performed using the acid 46 or intermixing with other acids including for example acid 62, to form successive oligomers where n=2 to infinity (a hexamer is shown in scheme 5 ) to obtain large carbopeptoid libraries. Next dicyclohexylcarbodiimide ( 1 .2 equivalents) is added and the reaction is stirred for 14 hours. The mixture is diluted with ether, filtered and the filtrate is washed with aqueous NaHCO3 (2X), water (2X), and brine (2X). The organic phase is dried over MgSO4 and then concentrated. Purification by flash column chromatography affords compound 67. scheme 5 step 1
Preparation of 68
Figure imgf000065_0002
To a stirred solution of 67 ( 1.0 equivalents) in dimethylformamide (.10 Molar) at 25 °C, is added piperidine (1.1 equivalents). The reaction is stirred for 1 hour and is then diluted with ether, and washed with aqueous CUSO4 (2X), water (2X), and brine (2X). The organic phase is dried over MgSO4 and then concentrated . Purification by flash column chromatography affords compound 68. Note: nume rous iterations can be performed using variable length oligomers of 68 to form peptoid oligomers where n=2 to infinity (a hexamer is shown in scheme 5). scheme 5 step 2
Preparation of 69
Figure imgf000065_0001
To a stirred solution of the acid 62 ( 1 .0 equivalents) and the amine 68 ( 1.1 equivalents) in dimethylformamide (.10 Molar) at 25 °C, is added 1-hydroxybenzotriazole (HOBT; 1.1 equivalents). Note: numerous iterations can be performed using the acid 62, or intermixing with other acids including for example acid 46, to form successive oligomers where n=2 to infinity (a hexamer is shown in scheme 5 ) to obtain large carbopeptoid libraries. Next dicyclohexylcarbodiimide ( 1.2 equivalents) is added and the reaction is stirred for 14 hours. The mixture is diluted with ether, filtered and the filtrate is washed with aqueous NaHCO3 (2X), water (2X), and brine (2X). The organic phase is dried over MgSO4 and then concentrated. Purification by flash column chromatography affords compound 69. scheme 5 step 1
Preparation of 70
Figure imgf000066_0001
To a stirred solution of 69 ( 1 .0 equivalents ) in dimethylformamide (. 10 Molar) at 25 °C, is added piperidine ( 1.1 equivalents). The reaction is stirred for 1 hour and is then diluted with ether, and washed with aqueous CUSO4 (2X), water (2X), and brine (2X). The organic phase is dried over MgSO4 and then concentrated . Purification by flash column chromatography affords compound 70. Note : nume rous iterations can be performed using variable length oligomers of 70 to form peptoid oligomers where n=2 to infinity (a hexamer is shown in scheme 5). scheme 5 step 2
Preparation of 71
Figure imgf000067_0001
To a stirred solution of the acid 46 ( 1.0 equivalents) and the amine 70 ( 1 . 1 equivalents) in dimethylformamide ( . 10 Molar) at 25 ºC. is added 1 -hydroxybenzotriazole (HOBT; 1 .1 equivalents). Note: numerous iterations can be performed using the acid 46 or intermixing with other acids including for example acid 62. to form successive oligomers where n-2 to infinity (a hexamer is shown in scheme 5 ) to obtain large carbopeptoid libraries. Next dicyclohexylcarbodiimide ( 1 .2 equivalents) is added and the reaction is stirred for 14 hours. The mixture is diluted with ether, filtered and the filtrate is washed with aqueous NaHCO3 (2X), water (2X), and brine (2X).
The organic phase is dried over MgSO4 and then concentrated.
Purification by flash column chromatography affords compound 71. scheme 5 step 1
Preparation of 72
Figure imgf000069_0001
To a stirred solution of 71 ( 1.0 equivalents) in dimethylformamide (.10 Molar) at 25 °C, is added piperidine ( 1.1 equivalents). The reaction is stirred for 1 hour and is then diluted with ether, and washed with aqueous CUSO4 (2X), water (2X), and brine (2X). The organic phase is dried over MgSO4 and then concentrated . Purification by flash column chromatography affords compound 72. Note: n ume rous iterations can be performed using variable length oligomers of 72 to form peptoid oligomers where n=2 to infinity (a hexamer is shown in scheme 5). scheme 5 step 2
Preparation of 74
Figure imgf000069_0002
To a stirred solution of 72 ( 1.0 equivalents) in acetonitrile (.50 Molar) is added an HF pyridine solution (.50 M) from Aldrich chemical company. The reaction is allowed to stir for five hours and is then condensed. The crude 73 oligomer is then resuspended in p-dioxane (.50 Molar) to which is added a 3.0 Molar solution of NaOH (3.0 equivalents). The reaction is stirred for 1 hour at 50 °C and is then quenched with aqueous NH4CI (2X) and subsequently lyophilized. Purification by HPLC chromatography affords compound 74. scheme 5 Preparation of 76
Figure imgf000070_0001
To a solution of β -D-Glucose pentaacetate 36 i n nitromethane from Aldrich company ( .13 Molar), is added trimethylsilylcyanide ( 3.0 equivalents) and then tin tetrachloride (.02 equivalents). Note: other pyranose sugars such as β -D-Mannose, β-D-Galactose pentaacetate and other lewis acids such as BF3 O Et 2 may be used for alternative derivatives. The mixture is stirred for one hour and then an aqueous solution of sodium acetate was added to hydrolyze the remaining trimethylsilylcyanide. The mixture is evaporated and the remaining oil is resuspended in dichloromethane and washed with sodium acetate solution (IX), water (IX), brine ( IX) and then dried over magnesium sulphate and concentrated. The crude product is next dissolved in ethanol (or methanol if the O-methyl glycoside is desired as in scheme 20), (0.15 M) and then concentrated H2 S O 4 (0.01 equivalents) is added. The reaction mixture is heated to 85 °C for eight hours. The solution is next concentrated in vacuo and purification by flash column chromatography affords compound 76. scheme 6; 76, scheme
20 (as the O-methyl glycoside).
Preparation of 78
Figure imgf000071_0001
To Tetrol 76 ( 1 .0 equivalents) in pyridine (.10 Molar), is added dimethyoxytntylchloride (DMT chloride) (2.5 equivalents) at 0 °C. The reaction is stirred for 2 hours and then diluted with diethylether and washed with ammonium chloride (2X), copper sulfate (2X), brine ( 1 X), dried over MgSO4 and concentrated. Next a solution of the crude intermediate ( 1.0 equivalents) is dissolved in methylene chloride ( . 10 Molar) and diisopropylethylamine (4.4 equivalents) is added at 0 °C. Subsequent addition of triethylsilyl trifluoromethanesulfonate (4.4 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X), brine ( IX) and then dried (MgSO4) and concentrated. Purification by flash column chromatography affords compound 78, scheme 6; 78, scheme 20 (as the O-methyl glycoside).
Preparation of 80
Figure imgf000072_0001
To a solution of 78 ( 1.0 equivalents) in methylene chloride (.10 Molar) is added a 1.0 M solution of DIBALH in methylene chloride from Aldrich chemical company ( 1.2 equivalents) at 0 °C. Subsequent stirring for 2 hours is followed by dilution with diethylether and washing with sodium-potassium tartrate (2X), brine ( IX) and then MgSO4. The solution is then concentrated and purification by flash column chromatography affords compound 80. scheme 6
Preparation of 82
Figure imgf000073_0001
To a solution of 80 ( 1.0 equivalents) in methylene chloride (.10 M), is added diisopropylethylamine (4.0 equivalents) at 25°C. The reaction is stirred for 5 minutes and then 2-cyanoethyl- N, N-diisopropyl-chlorophosphoramidite ( 1 .5 equivalents) is added, as prepared from the procedures of Sinha et al. Nu c l. Acids Res. 1 984 , 12, 4539. After 15 minutes the reaction is complete and is next diluted with ether and next washed with brine ( I X) and is then dried (MgSO4 ) and concentrated. Purification by flash column chromatography (silica, 30% ethyl acetate in petroleum ether) affords compound 82 (66% yield) . scheme 6
Preparation of 84
Figure imgf000074_0001
To 80 (1.0 equivalents) in methylene chloride (.10 Molar) at 0 °C, is added diisopropylethylamine ( 1.1 equivalents ). Subsequent addition of triethylsilyl trifluoromethanesulfonate (1.1 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X), brine ( IX) and then dried (MgSO4) and concentrated. The crude is then resuspended in nitromethane and exposed to 10% CI3COOH ( 1.1 equivalents) in THF ( .10 Molar). The reaction is stirred at 0 °C for 2 hours and is then diluted with ether and washed with sodium bicarbonate (2X), brine ( 1 X) and then dried (MgSO4) and concentrated. Purification by flash column chromatography affords compound 84. scheme 6
Preparation of 86
Figure imgf000075_0001
To a solution of N-phthalamido-D-Glucosamine tetraacetate 48 in nitromethane (.13 Molar), is added trimethylsilyl cyanide (3.0 equivalents) and then SnCl4 (.02 equivalents). The mixture is stirred for one hour and then an aqueous solution of sodium acetate was added to hydrolyze the remaining trimethylsilyl cyanide. The mixture is evaporated and the remaining oil is resuspended in dichloromethane and washed with sodium acetate solution (IX), water (IX), brine ( IX) and then dried over magnesium sulphate and concentrated. The crude product is next dissolved in ethanol (0.15 M) and then concentrated H2S O4 (0.04 equivalents) is added. The reaction mixture is heated to 85 °C for eight hours. The solution is next concentrated in vacuo and is then resuspended in methanol (.10 M) and acetic anhydride ( 1.1 equivalents) from Aldrich company is added in one step. After 2 hours, condensation and purification by flash column chromatography affords compound 86. scheme 7 Preparation of 88
Figure imgf000076_0001
To Triol 86 (1.0 equivalents) in pyridine (.10 Molar), is added dimethyoxytntylchloride (DMT chloride) (2.5 equivalents) at 0 °C. The reaction is stirred for 2 hours and then diluted with diethylether and washed with ammonium chloride (2X), copper sulfate (2X), brine ( 1X), dried over MgSO4 and concentrated. Next a solution of the crude intermediate ( 1 .0 equivalents) is dissolved in methylene chloride (.10 Molar) and diisopropylethylamine (3.3 equivalents) is added at 0 °C. Subsequent addition of triethylsilyl trifluoromethanesulfonate (3.3 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X), brine ( IX) and then dried (MgSO4) and concentrated. Purification by flash column chromatography affords compound 88. scheme 7
Preparation of 90
Figure imgf000077_0002
To a solution of 88 (1.0 equivalents) in methylene chloride (.10 Molar) is added a 1.0 M solution of DIBALH in methylene chloride from Aldrich chemical company ( 1.2 equivalents) at 0 °C. Subsequent stirring for 2 hours is followed by dilution with diethylether and washing with sodium-potassium tartrate (2X), brine ( IX) and then MgSO4. The solution is then concentrated and purification by flash column chromatography affords compound 90. scheme 7
Preparation of 92
Figure imgf000077_0001
To a solution of 90 (1 .0 equivalents) in methylene chloride (.10 M), is added diisopropylethylamine (4.0 equivalents) at 25 °C. The reaction is stirred for 5 minutes and then 2-cyanoethyl-N, N-diisopropyl-chlorophosphoramidite ( 1.5 equivalents) is added, as prepared from the procedures of Sinha et al. Nuc l. Acids Res. 1984 , 12, 4539. After 15 minutes the reaction is complete and is next diluted with ether and next washed with brine ( IX) and is then dried (MgSO4 ) and concentrated. Purification by flash column chromatography (silica, 30% ethyl acetate in petroleum ether) affords compound 92 (66% yield). scheme 7
Preparation of 94
Figure imgf000078_0001
To 90 ( 1.0 equivalents) in methylene chloride (.10 Molar) at 0 °C, is added diisopropylethylamine ( 1 .1 equivalents).
Subsequent addition of triethylsilyl trifluoromethanesulfonate ( 1.1 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X), brine ( IX) and then dried (MgSO4) and concentrated. The crude is then resuspended in nitromethane and exposed to 10% CI3COOH ( 1.1 equivalents) in THF (.10 Molar). The reaction is stirred at 0 °C for 2 hours and is then diluted with ether and washed with sodium bicarbonate (2X), brine (IX) and then dried (MgSO4) and concentrated. Purification by flash column chromatography affords compound 94 . scheme 7
Preparation of 98 (homodimer scheme 8)
To a solution of 94 ( 1.0 equivalents) in methylene chloride (.10 M), is added 1-H-tetrazole from Aldrich company ( 10.0 equivalents) at 25 °C. Next, a solution of 82 (3.0 equivalents) in methylene chloride ( 1.0 M), is added dropwise with stirring at
25 °C. After 25 minutes, the mixture is cooled to 0 °C and I2 (4.0 equivalents), 2,6 lutidine (4.0 equivalents) in TΗF ( 1.0 M) is added to oxidize the phosphoamidate to the phosphate (Alternatively m-chloroperoxybenzoic acid (4.5 equivalents) is added). The reaction is next stirred for an additional 5 minutes and is next diluted with ether and washed with brine ( IX) and dried (MgSO4 ) and concentrated. Purification by flash column chromatography and then the product is suspended in acetic acid-tetrahydrofuran-water (3 : 1 : 1 ), (.01 M) and stirred for 18 hours at 25 °C. The reaction is then diluted with ether and washed with NaΗCO3 (3X), brine ( IX) and dried (MgSO4 ) and concentrated. Purification by flash column chromatography affords compound 98 (scheme 8). Preparation of 102 (heterotrimer scheme 8)
To a solution of 98 ( 1.0 equivalents) in methylene chloride (.10 M), is added 1-H-tetrazole from Aldrich company ( 10.0 equivalents) at 25 °C. Next, a solution of 92 (3.0 equivalents) in methylene chloride ( 1.0 M), is added dropwise with stirring at
25 °C. After 25 minutes, the mixture is cooled to 0 °C and I2 (4.0 equivalents), 2,6 lutidine (4.0 equivalents) in TΗF ( 1.0 M) is added to oxidize the phosphoamidate to the phosphate (Alternatively m-chloroperoxybenzoic acid (4.5 equivalents) is added). The reaction is next stirred for an additional 5 minutes and is next diluted with ether and washed with brine ( IX) and dried (MgSO4) and concentrated. Purification by flash column chromatography and then the product is suspended in acetic acid-tetrahydrofuran-water (3: 1 : 1 ), (.01 M) and stirred for 18 hours at 25 °C. The reaction is then diluted with ether and washed with NaΗCO3 (3X), brine ( IX) and dried (MgSO4 ) and concentrated. Purification by flash column chromatography affords compound 102 (scheme 8). Preparation of 106 (heterotetramer scheme 8)
To a solution of 102 ( 1.0 equivalents) in methylene chloride (.10 M), is added 1-H-tetrazole from Aldrich company ( 10.0 equivalents) at 25 °C. Next, a solution of 82 (3.0 equivalents) in methylene chloride ( 1.0 M), is added dropwise with stirring at 25 °C. After 25 minutes, the mixture is cooled to 0 °C and I2 (4.0 equivalents), 2,6 lutidine (4.0 equivalents) in THF ( 1.0 M) is added to oxidize the phosphoamidate to the phosphate (Alternatively m-chloroperoxybenzoic acid (4.5 equivalents) is added). The reaction is next stirred for an additional 5 minutes and is next diluted with ether and washed with brine ( 1 X) and dried (MgSO4 ) and concentrated. Purification by flash column chromatography and then the product is suspended in acetic acid-tetrahydrofuran-water (3: 1 : 1 ), (.01 M) and stirred for 18 hours at 25 °C. The reaction is then diluted with ether and washed with NaHCO3 (3X), brine ( IX) and dried (MgSO4) and concentrated. Purification by flash column chromatography affords compound 106 (scheme 8). Preparation of 110 (heteropentamer scheme 8)
To a solution of 106 ( 1 .0 equivalents) in methylene chloride ( .10 M), is added 1 -H-tetrazole from Aldrich company ( 10.0 equivalents) at 25 °C. Next, a solution of 92 (3.0 equivalents) in methylene chloride ( 1.0 M), is added dropwise with stirring at 25 °C. After 25 minutes, the mixture is cooled to
0 °C and I2 (4.0 equivalents), 2,6 lutidine (4.0 equivalents) in TΗF ( 1.0 M) is added to oxidize the phosphoamidate to the phosphate (Alternatively m-chloroperoxybenzoic acid (4.5 equivalents) is added). The reaction is next stirred for an additional 5 minutes and is next diluted with ether and washed with brine (1X) and dried (MgSO4) and concentrated. Purification by flash column chromatography and then the product is suspended in acetic acid-tetrahydrofuran-water (3:1:1), (.01 M) and stirred for 18 hours at 25 °C. The reaction is then diluted with ether and washed with NaHCO3 (3X), brine (IX) and dried (MgSO4) and concentrated. Purification by flash column chromatography affords compound 110 (scheme 8). Preparation of 114 (heterohexamer scheme 8)
To a solution of 110 (1.0 equivalents) in methylene chloride (.10 M). is added 1-H-tetrazole from Aldrich company (10.0 equivalents) at 25 °C. Next, a solution of 82 (3.0 equivalents) in methylene chloride (1.0 M), is added dropwise with stirring at 25 °C. After 25 minutes, the mixture is cooled to
0 °C and h (4.0 equivalents), 2,6 lutidine (4.0 equivalents) in TΗF (1.0 M) is added to oxidize the phosphoamidate to the phosphate (Alternatively m-chloroperoxybenzoic acid (4.5 equivalents) is added). The reaction is next stirred for an additional 5 minutes and is next diluted with ether and washed with brine (1X) and dried (MgSO4) and concentrated. Purification by flash column chromatography and then the product is suspended in acetic acid-tetrahydrofuran-water (3:1:1), (.01 M) and stirred for 18 hours at 25 °C. The reaction is then diluted with ether and washed with NaHCO3 (3X), brine ( 1X) and dried (MgSO4) and concentrated. Purification by flash column chromatography affords compound 114 (scheme 8). Preparation of 116 (heterohexamer scheme 8)
To a solution of 114 (1.0 equivalents) in methylene
chloride (.10 M), is added a solution of HF-pyridine (1.0 M) at 0
°C. The reaction is next stirred for an additional 30 minutes and
is next diluted with ether and washed with a saturated solution
of sodium bicarbonate (3X), copper sulfate solution to remove
the pyridine (2X) brine (1X), dried (MgSO4) and concentrated.
Purification by flash column chromatography and then the
product is resuspended in concentrated aqueous ammonium
hydroxide and acetonitrile (1 : 1), (.1 M total). The reaction is
then stirred for 2 hours at 50 °C and is subsequently diluted
with ether and washed with NaHCO3 (3X), brine ( 1X) and dried
(MgSO4) and concentrated. Purification by flash column
chromatography affords compound 116 scheme 8.
Figure imgf000084_0001
Preparation of 125
Figure imgf000085_0001
To a solution of β-D-Glucose pentaacetate in nitromethane from Aldrich company (.13 Molar), is added trimethylsilylcyanide (3.0 equivalents) and then borontrifluoride etherate (.02 equivalents). Note: other pyranose sugars such as β-D-Mannose, β-D-Galactose pentaacetate and other lewis acids such as SnCl4, may be used for alternative derivatives. The mixture is stirred for one hour and then an aqueous solution of sodium acetate was added to hydrolyze the remaining trimethylsilylcyanide. The mixture is evaporated and the remaining oil is resuspended in dichloromethane and washed with sodium acetate solution (1X), water (1X), brine (1X) and then dried over magnesium sulphate and concentrated. The crude solid is then recrystallized from methanol to yield 125 (also 37) as a white solid (47%). scheme 9 step a Preparation of 126
Figure imgf000086_0002
To a solution of 125 in methanol (.13 Molar), is added sodium methoxide (0.3 equivalents) and the reaction mixture is stirred for two hours at room temperature. The dark brown solution is then concentrated in vacuo to give a dark brown syrup of compound 126 which is carried on without purification as a crude oil for the next step, scheme 9 step b
Preparation of 127
Figure imgf000086_0001
The crude product 126 is dissolved in 25% NaOH (0.5 M) and heated at reflux for 18 hours (vigorous reflux is necessary).
Next, the solution is diluted with an addition of water (0.1 M) and to this solution is added Amberlite 1 12120 resin (H+-form ) and is then stirred. The supernatant is then decanted and the resin is washed until the eluate is colorless. The eluate is then collected, condensed and azeotroped with MeOH which yields 127 as a crude, pale yellow syrup (47%).
Preparation of 130
Figure imgf000087_0002
The crude product 127 is next dissolved in methanol (0.15 M) and then concentrated HCl (0.01 equivalents) is added. The reaction mixture is heated to 85 °C for eight hours. The solution is next concentrated in vacuo and purification by flash column chromatography (silica, 20% methanol in ethyl acetate), affords compound 130 as a white solid (60% yield), scheme 9 step d
Preparation of 131
Figure imgf000087_0001
To a solution of 130 (1.0 equivalents) in dimethylformamide (.23 Molar), is added imidazole (2.5 equivalents) at 0 °C. Subsequent addition of tert-Butyl-dimethylsilylchloride (2.5 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X), brine (IX) and then dried (MgSO4) and concentrated. Purification by flash column chromatography (silica, 50% ethyl acetate) affords compound 131 as a white solid (93% yield), scheme 9 step e Note: the molecule can be protected with other primary directing protecting groups such as DMT (dimethoxytrityl), and TBDPS tert-butyldiphenly silyl, etc. Preparation of 132
Figure imgf000088_0001
To a solution of 131 (1.0 equivalents) in dimethylformamide (.23 M), is added Ag2O (6.0 equivalents) at 25 °C. Benzyl bromide (9.0 equivalents) is next added and the reaction is allowed to stir for 20 hours. The reaction is diluted with diethylether and washed with ammonium chloride (2X), brine ( 1 X) and then dried (MgSO4 ) and concentrated. Purification by flash column chromatography (silica, 20% ethyl acetate) affords compound 132 (83% yield), scheme 9 step f Note: the choice of the protecting group is relative and the molecule can be protected with other protecting groups at C2, C3, C4, such as PMB (paramethoxybenzyl), TES (triethylsilyl), TBS (tertbutyldimethylsilyl), etc.
Preparation of 134
Figure imgf000089_0001
To a solution of 132 ( 1 .0 equivalents) in tetrahydrofuran (.08 M), is added diisobutylaluminumhydride (DIBALH) (3.0 equivalents) at 0 °C. The reaction is stirred for 1 hour and then quenched with methanol and diluted with ether. The reaction is next worked-up with ammonium chloride (2X), brine ( 1X) and is then dried (MgSO4 ) and concentrated. Purification by flash column chromatography (silica. 20% ethyl acetate) affords compound 134 (66% yield), scheme 9 step g Preparation of 136
Figure imgf000090_0001
To a solution of 134 ( 1.0 equivalents) in pyridine ( 10.0 equivalents), is added naphthoyl chloride (3.0 equivalents) from Aldrich company (3.0 equivalents) at 25 °C. The reaction is stirred for 45 minutes and then diluted with ether and worked-up with a saturated solution of CUSO4 (2X), brine (IX) and is then dried (MgSO4) and concentrated. The crude product is then exposed to acetic acid/tetrahydrofuran/water (3: 1 : 1 ) at 25 °C and allowed to stir for 15 hours. The reaction is then diluted with ether and worked-up with brine (2X) and is then dried ( M g S O 4 ) and concentrated. Purification by flash column chromatography (silica. 20% ethyl acetate) affords compound 136 (95% yield). Note: alternatively, one could originally protect the C7 position as a DMT (dimethoxytrityl) functionality and protect the Cl position as a TES (triethyl silyl) group. Subsequent mild acid hydrolysis of the DMT group leads to the above compound with the TES group at the C1 position and a free hydroxyl at the C7 position, scheme 9 step h
Preparation of 138
Figure imgf000091_0001
To a solution of 134 ( 1 .0 equivalents) in methylene chloride ( . 1 0 M ). i s added diisopropylethylamine (4.0 equivalents) at 25 ºC. The reaction is stirred for 5 minutes and then 2-cyanoethyl- N,N-diisopropyl-chlorophosphoramidite ( 1.5 equivalents) is added, as prepared from the procedures of Sinha et al. Nucl. Acids Res. 1 984 , 12, 4539. After 15 minutes the reaction is complete and is next diluted with ether and next washed with brine ( 1 X) and is then dried (MgSO4 ) and concentrated. Purification by flash column chromatography (silica, 30% ethyl acetate in petroleum ether) affords compound 138 (66% yield), scheme 9 step i
It should be noted that the oligomerization process as shown below in scheme 9, uses the same C-glycoside 138 in an iterative fashion. The process can be extended however to include a pool of random or ordered C-glycosides as depicted in scheme 8.
Preparation of 140
Figure imgf000092_0001
To a solution of 136 ( 1.0 equivalents) in methylene chloride (.10 M), is added 1-H-tetrazole from Aldrich company ( 10.0 equivalents) at 25 °C. Next, a solution of 138 (3.0 equivalents) in methylene chloride ( 1.0 M), is added dropwise with stirring at 25 °C. After 25 minutes, the mixture is cooled to 0 °C and m-chloroperoxybenzoic acid (4.5 equivalents) is added. The reaction is stirred for an additional 5 minutes and is next diluted with ether and washed with brine ( I X) and dried ( M g S O 4 ) and concentrated. Purification by flash column chromatography (silica, 50% ethyl acetate in petroleum ether) affords compound 140 (97% yield), scheme 9 step j Note the process can iterate as many times as possible to build large carbonucleotide libraries. Preparation of 142
Figure imgf000093_0002
A solution of 1 40 ( 1.0 equivalents) in acetic acid- tetrahydrofuran-water (3: 1 : 1 ), (.01 M) is stirred for 18 hours at 25 °C. The reaction is then diluted with ether and washed with NaHCO 3 (3X), brine ( IX) and dried (MgSO4) and concentrated. Purification by flash column chromatography (silica, 60% ethyl acetate in petroleum ether) affords compound 142 (95% yield). scheme 9 step k Note the process can iterate as many times as possible to build large carbonucleotide libraries.
Preparation of 144
Figure imgf000093_0001
To a solution of 138 ( 1 .0 equivalents) in methylene chloride (.10 M), is added 1 -H-tetrazole from Aldrich company (10.0 equivalents) at 25 °C. Next, a solution of 142 (3.0 equivalents) in methylene chloride ( 1.0 M), is added dropwise with stirring at 25 °C. After 25 minutes, the mixture is cooled to 0 °C and m-chloroperoxybenzoic acid (4.5 equivalents) is added. The reaction is stirred for an additional 5 minutes and is next diluted with ether and washed with brine ( I X) and dried ( M g S O 4 ) and concentrated. Purification by flash column chromatography (silica, 50% ethyl acetate in petroleum ether) affords compound 144 (97% yield), scheme 9 step j Note the process can iterate as many times as possible to build large carbonucleotide libraries.
Preparation of 146
Figure imgf000095_0002
A solution of 144 (1.0 equivalents) in acetic acid- tetrahydrofuran-water (3:1:1), (.01 M total) is stirred for 18 hours at 25 °C. The reaction is then diluted with ether and washed with NaHCO3 (3X), brine (IX) and dried (MgSO4) and concentrated. Purification by flash column chromatography (silica, 60% ethyl acetate in petroleum ether) affords compound 146 (95% yield). scheme 9 step k Note the process can iterate as many times as possible to build large carbonucleotide libraries.
Preparation of 148
Figure imgf000095_0001
To a solution of 138 (1.0 equivalents) in methylene chloride (.10 M), is added 1-H-tetrazole from Aldrich company (10.0 equivalents) at 25 °C. Next, a solution of 146 (3.0 equivalents) in methylene chloride ( 1.0 M), is added dropwise with stirring at 25 °C. After 25 minutes, the mixture is cooled to 0 °C and m-chloroperoxybenzoic acid (4.5 equivalents) is added.
The reaction is stirred for an additional 5 minutes and is next diluted with ether and washed with brine ( 1 X) and dried ( M g S O 4 ) and concentrated. Purification by flash column chromatography (silica, 50% ethyl acetate in petroleum ether) affords compound 148 (97% yield), scheme 9 step j Note the process can iterate as many times as possible to build large carbonucleotide libraries.
Preparation of 150
Figure imgf000096_0001
A solution of 1 48 ( 1 .0 equivalents) in acetic acid- tetrahydrofuran-water (3: 1 : 1 ), ( .01 M total) is stirred for 18 hours at 25 °C. The reaction is then diluted with ether and washed with NaΗCO3 (3X), brine ( 1X) and dried (MgSO4 ) and concentrated. Purification by flash column chromatography (silica, 60% ethyl acetate in petroleum ether) affords compound 150 (95 % yield). scheme 9 step k Note the process can iterate as many times as possible to build large carbonucleotide libraries.
Preparation of 152
Figure imgf000097_0002
A solution of 1 50 ( 1.0 equivalents) is dissolved in concentrated aqueous ammonium hydroxide and acetonitrile ( 1 : 1 ), (.1 M total). The reaction is then stirred for 2 hours at 50 °C and is subsequently diluted with ether and washed with NaHC O 3 (3X), brine (IX) and dried (MgSO4) and concentrated.
Purification by flash column chromatography (silica, 80% ethyl acetate in petroleum ether) affords compound 152 (88% yield). scheme 9 step L Preparation of 154
Figure imgf000097_0001
A solution of 152 ( 1.0 equivalents) is dissolved in a mixture of ethanol-tetrahydrofuran-acetic acid (2: 1 : 1 ), ( .01 M total) at 25 °C. The mixture is next exposed to 10% Pd/C ( 1.0 equivalents) and is then subsequently capped with a hydrogen balloon at 1 atmosphere. The reaction is stirred for 72 hours and is then filtered through celite. The crude mixture is subsequently diluted with ether and washed with NaHCO3 (3X), brine ( 1X) and dried (MgSO4) and concentrated. Purification by flash column chromatography (silica, 100% ethyl acetate in petroleum ether) affords compound 154 (78% yield), scheme 9 step m
Preparation of 174 (R group = OTES, NPhth or NHAc)
To a solution of tetraacetate derived from 36 or 48 (glucose or glucosamine derived ) in methylene chloride (.1 molar) is added a 1.0 molar solution of Co2(CO)8 ( 1.5 equivalents ) in methylene chloride and diethylmethylsilane ( 1.5 equivalents) at 0 °C. To the stirring reaction mixture, a stream of carbon monoxide is bubbled at 1 ml per 10 seconds for 30 minutes. The reaction mixture is then quenched with water ( 1.5 equivalents), diluted with ether, washed with sodium bicarbonate (2x), brine ( 1x) and dried over magnesium sulfate. The crude is purified by column chromatography and affords product 174.
Preparation of 176 ( R group = OTES, NPhth or NHAc)
To a solution ol compound 174 in acetonitrile/water ( 1 : 1 ratio,
.1 molar combined ), is added RuCl3 (.03 equiv.) and NaIO4 (4.0 equiv.) at 25 °C and the muddy black mixture is allowed to stir for 1.5 h. The mixture is then diluted with ether (25 mL), washed with water ( 2X 5.0 mL) and brine ( 1X 5 mL). The aqueous layer is back extracted (2X), recombined, and the organic layer was then dried MgSO4 and evaporated. Purification by flash column chromatography yields the desired product 176.
Preparation of 178 (R group = OTES, NPhth or NHAc)
A solution of triacetate 176 ( 1.0 equiv.) in methanol (0.5 M), is treated with NaOMe (0.4 equiv.) and allowed to stir at 25 °C for 24 h. The reaction mixture is then condensed and purified by flash column chromatography to afford compound 178.
Preparation of 180 (R group = OTES, NPhth or NHAc)
To triol 178 ( 1.0 equivalents) in pyridine (.10 Molar), is added dimethyoxytntylchloride (DMT chloride) ( 1.5 equivalents) at 0°C. The reaction is stirred for 2 hours and then diluted with diethylether and washed with ammonium chloride (2X). copper sulfate (2X), brine ( 1X), dried over MgSO4 and concentrated. Next a solution of the crude intermediate ( 1.0 equivalents) is dis solved i n methylene ch loride ( . 1 0 Molar) and diisopropylethylamine (3.3 equivalents) is added at 0 °C .
Subsequent addition of triethylsilyl trifluoromethanesulfonate (3.3 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X). brine ( 1X) and then dried (MgSO4) and concentrated. Purification by flash column chromatography affords the intermediate acid, which is then resuspended in THF ( 1.0 M) and exposed to a 1.0 M solution of BH3 -THF ( 1 .5 equivalents) at 0 °C for 1 hour. The reaction is then quenched with methanol for an additional hour and the crude is then diluted with diethylether and washed with ammonium chloride (2X), brine (1X) and then dried (MgSO4) and concentrated. Purification by flash column chromatography affords the desired tetraprotected alcohol 180.
Preparation of 181 (R group = OTES, NPhth or NHAc)
To a solution of 180 ( 1.0 equivalents) in methylene chloride (.10 M), is added tetrazole (4.0 equivalents) at 25 °C. The reaction is stirred for 5 minutes and then 2-cyanoethyl-N, N-dii sopropy l-chlorophosphoramidite ( 1.5 equiv.) is added, as prepared from the procedures of Sinha et al. Nucl. Acids Res. 1984 , 12, 4539. After 15 minutes the reaction is complete and is next diluted with ether and next washed with brine ( IX) and is then dried ( M g S O 4 ) and concentrated. Purification by flash column chromatography (silica, 30% ethyl acetate in petroleum ether) affords compound 181 (66% yield), scheme 21
Preparation of 182 (R group = OTES. ΝPhth or ΝHAc)
To a solution of triol 178 (.0 equiv.) in CH2Cl2 (-5 M) at 0 °C. was added triethylamine (1.2 equiv.), 4-DMAP (.10 equiv.) and then TOSCl ( 1.1 equiv.). The reaction is stirred for 1 h and then is quenched with saturated ammonium chloride ( 1.5 mL), diluted with ethyl acetate (25 mL), washed with water (2X 5 mL). brine ( 1X 5 mL), back-extracted (2X), recombined, dried (MgSO4 ) and evaporated. The compound is purified by flash column chromatography and then a solution of the crude intermediate ( 1.0 equivalents) is dissolved in methylene chloride (.10 Molar) and diisopropylethylamine (2.2 equivalents) is added at 0 °C. Subsequent addition of triethylsilyl trifluoromethanesulfonate
(2.2 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X), brine ( 1X) and then dried (MgSO4) and concentrated. Purification by flash column chromatography affords the protected tosylate/acid 182.
Preparation of 183 (R group = OTES. NPhth or NHAc)
To a solution of triol 182 (.0 equiv.) in CH2Cl2 (.5 M) at 0 °C, is added sodium-azide ( 1.2 equiv.) from Aldrich chemical company at 0 °C. The reaction is stirred for 1 h and then is quenched with saturated ammonium chloride ( 1 .5 mL), diluted with ethyl acetate (25 mL), washed with water (2X 5 mL), brine ( 1X 5 mL), back-extracted (2X), recombined, dried (MgSO4) and evaporated. The compound is purified by flash column chromatography and affords compound 183.
Preparation of 185 (R group = OTES, NPhth or NHAc)
A solution of 183 (1.0 equivalents) in ethanol (.01 M total) at 25°C is exposed to 10% Pd(OH)2-C (0.1 equivalents) and is then subsequently capped with a hydrogen balloon at 1 atmosphere. The reaction is stirred for 72 hours and is then filtered through celite. The crude mixture is subsequently diluted with ether and washed with NaHCO3 (3X), brine (1X) and dried (MgSO4) and concentrated. Purification by flash column chromatography affords compound 185 scheme 21.
Preparation of 191
Figure imgf000102_0001
A solution of starting material 1 90 as disclosed by
Schmidt, R. R. et al. (Liebigs Ann. Chem. 1 987 , 825), ( 1.0 equivalents) is dissolved in a mixture of ethanol-tetrahydrofuran-acetic acid (2: 1 : 1 ), (.01 M total) at 25 °C. The mixture is next exposed to 10% Pd/C ( 1.0 equivalents) and is then subsequently capped with a hydrogen balloon at 1 atmosphere. The reaction is stirred for 72 hours and is then filtered through celite. The crude mixture is subsequently diluted with ether and washed with NaHCO3 (3X), brine ( 1X) and dried (MgSO4) and concentrated. Purification by flash column chromatography (silica, 100% ethyl acetate in petroleum ether) affords compound 191. scheme 22 step a
Preparation of 192
Figure imgf000104_0001
To a solution of 191 ( 1.0 equivalents) in methylene chloride (.10 Molar) is added tosylchloride ( 1.2 equivalents) at 0 °C. Subsequent addition of triethylamine ( 1.5 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X), brine ( 1X) and then dried (MgSO4) and concentrated to afford the crude tosylate. Next a solution of the crude intermediate ( 1.0 equivalents) is dissolved in methylene chloride (.10 Molar) and diisopropylethylamine (3.3 equivalents) is added at 0 °C. Subsequent addition of triethylsilyl trifluoromethanesulfonate (3.3 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X), brine ( 1X) and then dried (MgSO4) and concentrated. Purification by flash column chromatography affords compound 192. scheme 22 step b Preparation of 193
Figure imgf000105_0002
To a solution of 192 ( 1.0 equivalents) in methylene chloride (.10 Molar) is added sodium azide from Aldrich chemical company ( 1.2 equivalents) at 0 °C. Subsequent stirring for 2 hours is followed by dilution with diethylether and washing with ammonium chloride (2X), brine ( 1 X) and then M g S O4. The solution is then concentrated and purification by flash column chromatography affords compound 193. scheme 22 step c
Preparation of 194
Figure imgf000105_0001
To solution of 193 in CCl4 (.33 M), CH3CN (.33 M) and water (.22 M) at 0 °C is added RuCl3 (.03 equiv.) and NaIO4 (4.0 equiv.) and the muddy black mixture is allowed to stir for 1.5 h. The mixture is then diluted with ether (25 mL), washed with water (2X 5.0 mL) and brine ( 1X 5 mL). The aqueous layer is back extracted (2X), recombined, and the organic layer iss then dried MgSO4 and evaporated. Purification by flash column chromatography affords the compound 194. scheme 22 step d
Preparation of 196
Figure imgf000106_0001
A solution of 194 ( 1.0 equivalents) is dissolved in ethanol (.01 M total) at 25 °C. The mixture is next exposed to 10% Pd/C ( .1 equivalents) and is then subsequently capped with a hydrogen balloon at 1 atmosphere. The reaction is stirred for 72 hours and is then filtered through celite. The crude mixture is subsequently diluted with ether and washed with NaHCO3 (3X), brine ( 1 X) and dried (MgSO4 ) and concentrated. Next, to a solution of crude amine ( 1.0 equivalents) in methylene chloride (.10 Molar), is added sodium bicarbonate (2.0 equivalents) at 0
°C. Subsequent addition of 9-fluorenylmethyl chloroformate (FMOC-Cl, 1.2 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X), brine (1X) and then dried (MgSO4) and concentrated. Purification by flash column chromatography affords compound 196. scheme 22 steps e-f
Preparation of 197
Figure imgf000108_0001
To Tetrol 191 (1.0 equivalents) in pyridine (.10 Molar), is added dimethyoxytritylchloride (DMT chloride) (2.5 equivalents) at 0 °C. The reaction is stirred for 2 hours and then diluted with diethylether and washed with ammonium chloride (2X), copper sulfate (2X). brine (1X), dried over MgSO4 and concentrated. Next a solution of the crude intermediate (1.0 equivalents) is dissolved in methylene chloride (.10 Molar) and diisopropylethylamine (3.3 equivalents) is added at 0 °C. Subsequent addition of triethylsilyl trifluoromethanesulfonate (3.3 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X), brine (1X) and then dried (MgSO4) and concentrated. Purification by flash column chromatography affords compound 197. scheme 22 step g
Preparation of 198
Figure imgf000109_0001
To solution of 197 in CCI4 (.33 M), CH3 CN (.33 M) and water (.22 M) at 0 °C is added RuCl3 (.03 equiv.) and NaIO4 (4.0 equiv.) and the muddy black mixture is allowed to stir for 1.5 h. The mixture is then diluted with ether (25 mL), washed with water (2X 5.0 mL) and brine ( 1X 5 mL). The crude is then resuspended in a mixture of methylene chloride/water ( 1 : 1 , .1 M total ) and diazomethane ( 1.2 equivalents) is gradually dropped into the flask via an addition funnel at the rate of 1 drop/10 seconds After complete addition the mixture is diluted with ether, washed with brine (2X) and the aqueous layer is back extracted ( 2X ) recombined, and the organic layer is then dried MgSO4 and evaporated. Purification by flash column chromatography affords the compound 198. scheme 22 step h Preparation of 200
Figure imgf000110_0002
To a solution of 198 ( 1.0 equivalents) in methylene chloride (.10 Molar) is added a 1.0 M solution of DIBALH in methylene chloride from Aldrich chemical company ( 1 .2 equivalents) at 0 °C. Subsequent stirring for 2 hours is followed by dilution with diethylether and washing with sodium-potassium tartrate (2X), brine ( 1 X) and then MgSO4. The solution is then concentrated and purification by flash column chromatography affords compound 200. scheme 22 step i
Preparation of 201
Figure imgf000110_0001
A solution of 200 ( 1 .0 equivalents) in tetrahydrofuran (.18 M) is treated with DPPA (diphenylphosphorylazide, 2.0 equivalents), triphenylphosphine ( 1.3 equivalents) and DIAD (diisopropyl-azo-dicarboxylate, 1.3 equivalents). The reaction is heated to 80 °C for 3 hours and then diluted with ether (2X) and washed with .5 M aqueous NaOH (2X). The organic layer is dried over MgSO4 and evaporated. Purification by flash column chromatography affords compound 201. scheme 22 step j
Preparation of 202
Figure imgf000111_0001
A solution of 201 ( 1.0 equivalents) is dissolved in ethanol (.01 M total) at 25 °C. The mixture is next exposed to 10% Pd/C (.1 equivalents) and is then subsequently capped with a hydrogen balloon at 1 atmosphere. The reaction is stirred for 72 hours and is then filtered through celite. The crude mixture is subsequently diluted with ether and washed with NaHCO3 (3X), brine ( 1 X) and dried (MgSO4 ) and concentrated. Next, to a solution of crude amine ( 1.0 equivalents) in methylene chloride (.10 Molar), is added sodium bicarbonate (2.0 equivalents) at 0 °C. Subsequent addition of 9-fluorenylmethyl chloroformate (FMOC-Cl, 1.2 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X), brine ( 1X) and then dried ( M g S O 4 ) and concentrated. Purification by flash column chromatography affords compound 202. scheme 22 step e
Preparation of 204
Figure imgf000112_0001
To a solution of 202 ( 1 .0 equivalents) in methylene chloride (.10 Molar) is added 10% HCOOH from Aldrich chemical company ( 1.2 equivalents) at 0 °C. Subsequent stirring for 2 hours is followed by dilution with diethylether and washing with sodium bicarbonate (2X), brine ( 1 X) and then MgSO4. The solution is then resuspended in r-BuOH (.10 M) and pH 7 buffer
(.10 M) and is then exposed to KMnO4 ( 1.2 equivalents) for 2 hours at 0 °C. The reaction mixture is next washed with sodium bicarbonate (2X), brine ( 1X) and then MgSO4. The organic layer is then concentrated and purified by flash column chromatography to afford compound 204. scheme 22 step k Preparation of 206
Figure imgf000113_0001
To Tetrol 205 ( 1.0 equivalents) (as disclosed by Petrus, L. et al. Chem. zvesti. 1982 , 36 , 103) in pyridine (.10 Molar), is added dimethyoxytntylchloride (DMT chloride) (2.5 equivalents) at 0 °C. The reaction is stirred for 2 hours and then diluted with diethylether and washed with ammonium chloride (2X), copper sulfate (2X), brine ( 1 X), dried over MgSO4 and concentrated.
Next a solution of the crude intermediate ( 1.0 equivalents) is di s solved in methy lene chloride ( . 10 Molar) and diisopropylethylamine (3.3 equivalents) is added at 0 °C. Subsequent addition of triethylsilyl trifluoromethanesulfonate (3.3 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X), brine ( 1X) and then dried (MgSO4) and concentrated. Purification by flash column chromatography affords compound 206. scheme 23 step a Preparation of 207
Figure imgf000114_0001
To a solution of 206 ( 1.0 equivalents) in diethylether (.08 M), is added lithiumaluminumhydride (LAH) ( 1.5 equivalents) at 30 °C. The reaction is refluxed for 2 hours and then quenched with methanol and diluted with ether. The reaction is next worked-up with sodium potassium tartrate (2X), brine ( 1 X) and is then dried (MgSO4) and concentrated. The crude mixture is resuspended in methylene chloride (.10 Molar) and to it is added sodium bicarbonate (2.0 equivalents) at 0 °C.
Subsequent addition of 9-fluorenylmethyl chloroformate (FMOC- Cl, 1.2 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X), brine ( 1 X) and then dried (MgSO4) and concentrated. Purification by flash column chromatography affords compound 207. scheme 23 step b
Preparation of 208
Figure imgf000115_0002
To a solution of 207 ( 1 .0 equivalents) in methylene chloride (.10 Molar) is added 10% HCOOH ( 1.1 equivalents). The reaction is stirred at 0 °C for 2 minutes and is then diluted with ether and washed with sodium bicarbonate (2X), brine ( 1 X) and then dried (MgSO4 ) and concentrated. Purification by flash column chromatography affords compound 208. scheme 23 step c
Preparation of 209
Figure imgf000115_0001
To solution of 208 in CCI4 (.33 M), CH3CN (.33 M) and water (.22 M) at 20 °C is added RuCl3 (.03 equiv.) and NaIO4
(4.0 equiv.) and the muddy black mixture is allowed to stir for
10 min. The mixture is then diluted with ether (25 mL), washed with water (2X 5.0 mL) and brine ( 1X 5 mL). The aqueous layer is back extracted (2X), recombined, and the organic layer iss then dried MgSO4 and evaporated. Purification by flash column chromatography affords the compound 209. scheme 23 step d
Preparation of 210
Figure imgf000116_0001
To a solution of 205 ( 1 .0 equivalents) in methylene chloride (.10 Molar) is added tosylchloride (1.2 equivalents) at 0 °C. Subsequent addition of triethylamine ( 1.5 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X), brine ( I X) and then dried (MgSO4) and concentrated to afford the crude tosylate. Next a solution of the crude intermediate ( 1.0 equivalents) is dissolved in methylene chloride (.10 Molar) and diisopropylethylamine (3.3 equivalents) is added at 0 °C. Subsequent addition of triethylsilyl trifluoromethanesulfonate
(3.3 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X), brine (IX) and then dried (MgSO4) and concentrated. Purification by flash column chromatography affords compound 210. scheme 23 step e Preparation of 211
Figure imgf000117_0001
To a solution of 210 ( 1.0 equivalents) in methylene chloride (.10 Molar) is added sodium azide from Aldrich chemical company ( 1.2 equivalents) at 0 °C. Subsequent stirring for 2 hours is followed by dilution with diethylether and washing with ammonium chloride (2X), brine ( 1 X) and then M g S O4. The solution is then concentrated and purification by flash column chromatography affords compound 211. scheme
23 step f
Preparation of 212
Figure imgf000118_0002
To solution of 211 in CCI4 (.33 M), CH3CN (.33 M) and water (.22 M) at 20 °C is added RuCl3 (.03 equiv.) and NaIO4 (4.0 equiv.) and the muddy black mixture is allowed to stir for 10 min. The mixture is then diluted with ether (25 mL), washed with water (2X 5.0 mL) and brine (1X 5 mL). The aqueous layer is back extracted (2X), recombined, and the organic layer iss then dried MgSO4 and evaporated. Purification by flash column chromatography affords the compound 212. scheme 23 step g Preparation of 213
Figure imgf000118_0001
A solution of 212 ( 1.0 equivalents) in ethanol (.01 M total) at 25 °C is exposed to 10% Pd/C (0.1 equivalents) and is then subsequently capped with a hydrogen balloon at 1 atmosphere. The reaction is stirred for 72 hours and is then filtered through celite. The crude mixture is subsequently diluted with ether and washed with NaHCO3 (3X), brine ( 1X) and dried (MgSO4) and concentrated. Purification by flash column chromatography affords compound 213. scheme 23 step h
Preparation of 214
Figure imgf000119_0001
Compound 213 is suspended in methylene chloride (.10
Molar) and to it is added sodium bicarbonate (2.0 equivalents) at 0 °C . S ubsequent addition of 9-fluorenylmethyl chloroformate ( FMOC-Cl, 1.2 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X), brine ( 1X) and then dried (MgSO4 ) and concentrated. Purification by flash column chromatography affords compound 214. scheme 23 step i Preparation of 215
Figure imgf000120_0001
To a solution of 205 ( 1.0 equivalents) in pyridine (.10
Molar), is added trimethylacetyl chloride (pivaloyl chloride) (2.5 equivalents) at 0 °C. The reaction is stirred for 2 hours and then diluted with diethylether and washed with ammonium chloride (2X), copper sulfate (2X), brine ( IX), dried over MgSO4 and concentrated. Next a solution of the crude intermediate
( 1.0 equivalents) is dissolved in methylene chloride (.10 Molar) and diisopropylethylamine (3.3 equivalents) is added at 0 °C. Subsequent addition of triethylsilyl trifluoromethanesulfonate (3.3 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X), brine ( 1X) and then dried (MgSO4) and concentrated. Purification by flash column chromatography affords compound 215. scheme 23 step j
Preparation of 216
Figure imgf000121_0001
To solution of 215 in CCI4 (.33 M), CH3CN (.33 M) and water (.22 M) at 20 °C is added RuCl3 (.03 equiv.) and NaIO4 (4.0 equiv.) and the muddy black mixture is allowed to stir for 10 min. The mixture is then diluted with ether (25 mL), washed with water (2X 5.0 mL) and brine ( 1X 5 mL). The aqueous layer is back extracted (2X), recombined, and the organic layer is then dried MgSO4 and evaporated. The crude is then resuspended in a mixture of methylene chloride/water ( 1 : 1 , .1 M total) and diazomethane ( 1 .2 equivalents) is gradually dropped into the flask via an addition funnel at the rate of 1 drop/10 seconds. After complete addition the mixture is diluted with ether, washed with brine (2X) and the aqueous layer is back extracted (2X) recombined. and the organic layer is then dried MgSO4 and evaporated. Purification by flash column chromatography affords the compound 216. scheme 23 step k Preparation of 217
Figure imgf000122_0002
To a solution of 216 ( 1.0 equivalents) in methylene chloride (.10 Molar) is added a 1.0 M solution of DIBALH in methylene chloride from Aldrich chemical company ( 1 .2 equivalents) at 0 °C. Subsequent stirring for 2 hours is followed by dilution with diethylether and washing with sodium-potassium tartrate (2X), brine ( 1 X) and then MgSO4. The solution is then concentrated and purification by flash column chromatography affords compound 217. scheme 23 step 1
Preparation of 218
Figure imgf000122_0001
To 217 ( 1.0 equivalents) in pyridine (.10 Molar), is added dimethyoxytritylchloride (DMT chloride) ( 1.1 equivalents) at 0°C. The reaction is stirred for 2 hours and then diluted with diethylether and washed with ammonium chloride (2X), copper sulfate (2X), brine ( 1X), dried over MgSO4 and concentrated. Purification by flash column chromatography affords compound 218. scheme 23 step m
Preparation of 220
Figure imgf000123_0001
To a solution of 218 ( 1.0 equivalents) in diethylether (.08
M), is added lithiumaluminumhydride (LAH) ( 1.5 equivalents) at 30 °C. The reaction is refluxed for 2 hours and then quenched with methanol and diluted with ether. The reaction is next worked-up with sodium potassium tartrate (2X), brine ( 1 X) and is then dried (MgSO4 ) and concentrated. Purification by flash column chromatography affords compound 220. scheme 23 step n
Preparation of 221
Figure imgf000124_0001
To Tetrol 205 ( 1.0 equivalents) in pyridine (.10
Molar), is added dimethyoxytntylchloride (DMT chloride) (2.5 equivalents) at 0 °C. The reaction is stirred for 2 hours and then diluted with diethylether and washed with ammonium chloride (2X), copper sulfate (2X), brine ( 1X), dried over MgSO4 and concentrated. Next a solution of the crude intermediate ( 1 .0 equivalents) is dissolved in methylene chloride (.10 Molar) and diisopropylethylamine (3.3 equivalents) is added at 0 °C. Subsequent addition of triethylsilyl trifluoromethanesulfonate (3.3 equivalents) is followed by stirring for 2 hours and then the reaction i s diluted with diethylether and washed with ammonium chloride (2X), brine ( 1X) and then dried (MgSO4 ) and concentrated. Purification by flash column chromatography affords compound 221. scheme 23 step a
Preparation of 222
Figure imgf000125_0002
To solution of 221 in CCI4 (.33 M), CH3CN (.33 M) and water (.22 M) at 20 °C is added RuCl3 (.03 equiv.) and NaIO4 (4.0 equiv.) and the muddy black mixture is allowed to stir for 10 min. The mixture is then diluted with ether (25 mL), washed with water (2X 5.0 mL) and brine ( 1X 5 mL). The aqueous layer is back extracted (2X), recombined, and the organic layer iss then dried MgSO4 and evaporated. Purification by flash column chromatography affords the compound 222. scheme 23 step o.
Preparation of 224
Figure imgf000125_0001
To a solution of 222 ( 1.0 equivalents) in diethylether (.08 M), is added lithiumaluminumhydride (LAH) (1.5 equivalents) at 30 °C. The reaction is refluxed for 2 hours and then quenched with methanol and diluted with ether. The reaction is next worked-up with sodium potassium tartrate (2X), brine (1X) and is then dried (MgSO4) and concentrated. Purification by flash column chromatography affords compound 224. scheme 23 step p
Preparation of 216
To a stirred solution of the acid 214 ( 1 .0 equivalents) in dimethylformamide ( .10 Molar) at 25 °C, is added 1 -hydroxybenzot ria zol e ( HOB T ; 1 . 1 equivalents) . Next dicyclohexylcarbodiimide ( 1.2 equivalents) is added and the reaction is stirred for 1 hour in the presence of an appropriately substituted solid support (N-(2-Aminoethyl)-3-amino-propyl glass; aminopolystyrene resin; aminopropyl glass; isothiocyanato glass, all with or without a linker extending from the amino group on the support etc. from Sigma Company). The mixture is then diluted with ether, filtered and the filtrate is washed with aqueous NaHCO3 ( 2X ). water (2X), and brine (2X). The organic phase is dried over MgSO4 and then concentrated.
Preparation of 226; 228; 230 or 232
To a stirred solution of the acid 214; 62; 215 or 62 ( 1 . 0 equivalents) and the amine 216; 226; 228 or 230 ( 1.1 equivalents) in dimethylformamide (.10 Molar) at 25 °C, is added 1-hydroxybenzotriazole (HOBT; 1.1 equivalents). N e x t dicyclohexylcarbodiimide ( 1.2 equivalents) is added and the reaction is stirred for 14 hours. The mixture is diluted with ether, filtered and the filtrate is washed with aqueous NaHCO3 (2X), water (2X), and brine (2X). The organic phase is dried over M g S O 4 and then concentrated. Purification by flash column chromatography and then reexposure of the intermediate compound (1.0 equivalents) in dimethyl-formamide (.10 Molar) at 25 °C, is added piperidine (1.1 equivalents). The reaction is stirred for 1 hour and is then diluted with ether, and washed with aqueous CuSO4 (2X), water (2X), and brine (2X). The organic phase is dried over MgSO4 and then concentrated. Purification by flash column chromatography affords compound
226; 228; 230 or 232, respectively, scheme 24
Preparation of 234
To a stirred solution of 232 ( 1.0 equivalents) in acetonitrile (.50 Molar) is added an HF pyridine solution (.50 M) from Aldrich chemical company. The reaction is allowed to stir for five hours and is then condensed. The crude 234 oligomer is then resuspended in p-dioxane (.50 Molar) to which is added a 3.0 Molar solution of NaOH (3.0 equivalents). The reaction is stirred for 1 hour at 50 °C and is then quenched with aqueous NH4C I (2X) and subsequently lyophilized. Purification by HPLC chromatography affords compound 234. scheme 24
Preparation of Peptoid Combinatorial libraries
(Scheme 500)
A depiction of the generation of a combinatorial library for oligopeptoid compounds is shown in scheme 500. The example uses an alphabet of eight D-aldose hexose sugars (other sugars groups such as the D/L ketoses and L-configurations of aldose hexoses, may be used) and carries the synthesis to a degree of three or 512 compounds. (The process can repeat itself to afford the library of desired size). Standard chemistry is shown and follows the reaction conditions as described above herein for peptoid synthesis. The solid support used is the standard N-(2-Aminoethyl)-3-amino-propyl glass support; amino-polystyrene resin; aminopropyl glass; isothiocyanato glass and others as purchased from Sigma company. All supports may be with or without a linker extending from the amino group on the support
(eg. succinate linkage, amide, ether, alkyl chain with terminal carbon activated as free alcohol, bromide etc.).
Figure imgf000130_0001
Figure imgf000131_0001
Figure imgf000132_0001
Figure imgf000133_0001
Figure imgf000134_0001
Figure imgf000135_0001
Preparation of Nucleotoid Combinatorial libraries
(Scheme 550)
A depiction of the generation of a combinatorial library for oligonucleotoid compounds is shown in scheme 550. The example uses an alphabet of eight D-aldose hexose sugars (other sugars groups such as the D/L ketoses and L-configurations of aldose hexoses. may be used) and carries the synthesis to a degree of three or 512 compounds. (The process can repeat itself to afford the library of desired size). Standard chemistry is shown and follows the reaction conditions as described above herein for carbonucleotoid synthesis. The solid support used is the standard N-( 2-Aminoethyl)-3-amino-propyl glass support; amino-polystyrenc resin; aminopropyl glass; isothiocyanato glass and others as purchased from Sigma company. All supports may be with or without a linker extending from the amino group on the support (eg. succinate linkage, amide, ether, alkyl chain with terminal carbon activated as free alcohol, bromide etc.).
Preparation of compound 2000. To a solution of 76 ( 1.0 equiv) was added methylene chloride (.1 M) and benzaldehyde (1.1 equiv), and the solution was exposed to ZnCl ( 1.1 equiv) at 25 °C and allowed to stir for 2.5 hour. The solution is then diluted with ether and then washed with a saturated solution of sodium bicarbonate (2X), water (2X), brine (1X) and then dried over MgSO4. The compound is purified by flash column chromatography to yield the desired benzylidene.
Figure imgf000138_0001
Figure imgf000139_0001
The benzylidene is then azeotroped with benzene (2X 100 mL) and then dried overnight under vacuum over P2O 5. A mixture of benzylidene, dibutyl tin oxide ( 1.2 equiv.) and dry methanol (.25 M) are heated at reflux for 4 h until the solution became clear and homogeneous. (An automatic stirring apparatus may be necessary.) The solvent is next removed in vacuo to give a foamy white tin complex which was then azeotroped with benzene (2X) and dried (2 h to overnight) under vacuum over P2O5. Next, anhydrous DMF (.2M) is added to redissolve the tin complex and then CsF ( 1.2 equiv.) and finally Benzyl bromide ( 1.5 equiv.) are added and then heated (40 °C) overnight. The clear solution is partially distilled under vacuum, (3.3 mm Hg, 75- 100 °C) to obtain 1/5 the original volume of solvent. Reaction mixture was then diluted with ethyl acetate (2L) and washed with a small amount of water (2X) to remove cesium salts. Aqueous layer is back extracted with ethyl acetate (3X) and then recombined with the organic layer which was then dried over MgSO4 and evaporated. Purification by flash column chromatography yields the desired benzyl ether 2000. F o r related chemistry see Nagashima, N.; Ohno. M. Chemistry Letters, Chem. Soc. of Japan 1987. 141. Preparation of compound 2010.
Procedure adopted from Johansson R.; Samuelsson; B. J. Chem. Soc , Chem. Commun. , 1 984 , 201. To a solution of the benzylidene acetal ( 1 equiv) and sodium cyanoborohydride (5 equiv.) in DMF (.125 M) containing powedered 3 angtrsom molecular sieves is added trifluoroacetic acid ( 10 equiv) and the reaction is allowed to stir at 0 °C until no starting material remains. Reaction mixture is then diluted with ethyl acetate (2L) and washed with a small amount of water (2X) and brine (2X). Aqueous layer is back extracted with ethyl acetate (3X) and then recombined with the organic layer which was then dried over MgSO4 and evaporated. Purification by flash column chromatography yields the desired benzyl ether 2010. Preparation of compound 2020.
To a solution of 2010 ( 1.0 equiv) was added methylene chloride (.1 M) and benzaldehyde ( 1 .1 equiv), and the solution was exposed to ZnCI ( 1.1 equiv) at 25 °C and allowed to stir for 2.5 hour. The solution is then diluted with ether and then washed with a saturated solution of sodium bicarbonate (2X), water (2X), brine ( 1 X) and then dried over MgSO4. The compound is purified by flash column chromatography to yield the desired benzylidene 2020. Preparation of compound 2030.
To a solution of alcohol 2020 (22.0 g, .1068 mol, 1.0 equiv.) in THF (0.5 M) at 0 °C, is added NaH (1.0 equiv., 35% dispersion in mineral oil) over several portions. The reaction mixture is warmed to room temperature and stirred 1h. Next, the reaction iss cooled to 0 °C and treated with benzyl bromide ( 1.0 equiv.) and stirred for 1.5 h. A saturated solution of ammonium chloride (50 mL) is added dropwise to quench the reaction mixture at 0 °C and the mixture was diluted with ethyl acetate, washed with water (2X), brine ( 1X), dried over MgSO4 and evaporated. Purification by flash column chromatography yields tribenzyl ether 2030.
Preparation of compound 2040.
Procedure as adopted from Hanessian S.; Organic Syntheses
1 987 , 243. To a suspension containing 1 .0 equivalent of benzylidene 2030 in one molar carbon tetrachloride and 1 , 1 ,2, 2-tetrachloroethane ( 1 .5 equivalent) i s added 1 .2 equivalents of N-bromosuccinimide and 0.5 equivalents of barium carbonate. The resulting suspension is heated at the reflux temperature of the mixture with mechanical stirring for a period of 2.5 hour and filtered while hot. The solution is washed with water (3X), then dried over anhydrous sodium sulfate and evaporated. Purification by flash column chromatography yields tribenzyl ether 2040.
Preparation of compound 2050.
To a solution of 2040 ( 1.0 equivalents) in methylene chloride (.10 M), is added diisopropylethylamine (4.0 equivalents) at 25 °C. The reaction is stirred for 5 minutes and then 2-cyanoethyl-N, N-diisopropyl-chlorophosphoramidite ( 1.5 equiv) is added, as prepared from the procedures of Sinha et al. Nucl. Acids Res. 1984, 12, 4539. After 15 minutes the reaction is
Figure imgf000144_0001
brine ( 1X) and is then dried (MgSO4 ) and concentrated. Purification by flash column chromatography (silica, 30% ethyl acetate in petroleum ether) affords compound 2050 (as shown in scheme 2000).
Preparation of compound 2060
To a solution of alcohol 2040 (1.0 equiv.) in THF (0.5 M) at 0 °C, is added NaH ( 1.0 equiv., 35% dispersion in mineral oil) over several portions. The reaction mixture is warmed to room temperature and stirred 1h. Next, the reaction is cooled to 0 °C and exposed to the solid support functionalized with a bromide linker or any reasonable leaving group attached ( 1.0 equiv.) and stirred for 2 hours. A saturated solution of ammonium chloride (50 mL) is added dropwise to quench the reaction mixture at 0 °C and the support was washed with ethyl acetate, 1 % NaOH in methanol (2X) to remove the benzoate and finally brine ( 1 X) to give 2 060 . The solid support used is the standard N-(2-Aminoethyl)-3-amino-propyl glass support; amino-polystyrene resin; aminopropyl glass; isothiocyanato glass and others as purchased from Sigma company. All supports may be with or without a linker extending from the amino group on the support (eg. succinate linkage, amide, ether, alkyl chain with terminal carbon activated as free alcohol, bromide etc.).
Figure imgf000146_0001
Figure imgf000147_0001
Preparation of compound 2070
To a solution of 76 (1.0 equiv) was added methylene chloride (.1 M) and benzaldehyde ( 1.1 equiv), and the solution was exposed to ZnCI (1.1 equiv) at 25 °C and allowed to stir for 2.5 hour. The solution is then diluted with ether and then washed with a saturated solution of sodium bicarbonate (2X), water (2X), brine (IX) and then dried over MgSO4. The compound is purified by flash column chromatography to yield the desired benzylidene.
Procedure adopted from Johansson R.; Samuelsson; B. J. Chem. Soc , Chem. Commun. , 1 984 , 201. To a solution of the benzylidene acetal (1 equiv) and sodium cyanoborohydride (5 equiv.) in DMF (.125 M) containing powedered 3 angtrsom molecular sieves is added trifluoroacetic acid (10 equiv) and the reaction is allowed to stir at 0 °C until no starting material remains. Reaction mixture is then diluted with ethyl acetate
(2L) and washed with a small amount of water (2X) and brine (2X). Aqueous layer is back extracted with ethyl acetate (3X) and then recombined with the organic layer which was then dried over MgSO4 and evaporated. Purification by flash column chromatography yields the desired benzyl ether 2070.
Preparation of compound 2080
To a solution of 2070 ( 1.0 equivalents) in methylene chloride (.10 Molar), is added triethylamine (1.1 equivalents) at 0 °C. Subsequent addition of tertbutyldiphenylsilylchloride ( 1 . 1 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X), brine ( IX) and then dried (MgSO4 ) and concentrated. Purification by flash column chromatography affords the TBDPS ether which is subsequently carried on as follows:
The TBDPS ether is then azeotroped with benzene (2X 100 mL) and then dried overnight under vacuum over P2 O 5. A mixture of benzylidene, dibutyl tin oxide ( 1.2 equiv.) and dry methanol
(.25 M) are heated at reflux for 4 h until the solution became clear and homogeneous. (An automatic stirring apparatus may be necessary.) The solvent is next removed in vacuo to give a foamy white tin complex which was then azeotroped with benzene (2X) and dried (2 h to overnight) under vacuum over
P2O5. Next, anhydrous DMF (.2M) is added to redissolve the tin complex and then CsF ( 1.2 equiv.) and finally Benzoyl bromide for the benzoate formation, ( 1.5 equiv.) are added and then heated (40 °C) overnight. The clear solution is partially distilled under vacuum, (3.3 mm Hg, 75- 100 °C) to obtain 1/5 the original volume of solvent. Reaction mixture was then diluted with ethyl acetate (2L) and washed with a small amount of water (2X) to remove cesium salts. Aqueous layer is back extracted with ethyl acetate (3X) and then recombined with the organic layer which was then dried over MgSO4 and evaporated. Purification by flash column chromatography yields the desired benzyl ether 2080. For related chemistry see Nagashima, N. ; Ohno, M. Chemistry Letters, Chem. Soc. of Japan 1987, 141.
Preparation of compound 2090
To a solution of alcohol 2080 (1.0 equiv.) in THF (0.5 M) at 0 °C, is added NaH (1.0 equiv., 35% dispersion in mineral oil) over several portions. The reaction mixture is warmed to room temperature and stirred 1h. Next, the reaction is cooled to 0 °C and treated with benzyl bromide ( 1.0 equiv.) and stirred for 1.5 h. The compound is then treated with tetrabutylammonium fluoride (2.0 equivalents) and allowed to stir for an additional 2 hours. A saturated solution of ammonium chloride (50 mL) is then added dropwise to quench the reaction mixture at 0 °C and the mixture was diluted with ethyl acetate, washed with water (2X). brine ( 1X), dried over MgSO4 and evaporated. Purification by flash column chromatography yields tribenzyl ether 2090. Preparation of compound 2100
To a solution of 2090 ( 1.0 equivalents) in methylene chloride (.10 M), is added diisopropylethylamine (4.0 equivalents) at 25 °C. The reaction is stirred for 5 minutes and then 2-cyanoethyl-N, N-diisopropyl-chlorophosphoramidite ( 1.5 equiv) is added, as prepared from the procedures of Sinha et al. Nucl. Acids Res. 1984, 12, 4539. After 15 minutes the reaction is complete and is next diluted with ether and next washed with brine ( 1X) and is then dried (MgSO4) and concentrated. Purification by flash column chromatography (silica, 30% ethyl acetate in petroleum ether) affords compound 2100 (as shown in scheme 2002).
Figure imgf000152_0001
Preparation of compound 2110
To a solution of alcohol 2090 (1.0 equiv.) in THF (0.5 M) at 0 °C, is added NaH ( 1.0 equiv., 35% dispersion in mineral oil) over several portions. The reaction mixture is warmed to room temperature and stirred 1h. Next, the reaction is cooled to 0 °C and exposed to the solid support functionalized with a bromide linker or any reasonable leaving group attached ( 1.0 equiv.) and stirred for 2 hours. A saturated solution of ammonium chloride (50 mL) is added dropwise to quench the reaction mixture at 0
°C and the support was washed with ethyl acetate, 1 % NaOH in methanol (2X) to remove the benzoate and finally brine ( IX) to give 2 1 1 0 . The solid support used is the standard N-(2-Aminoethyl)-3-amino-propyl glass support; amino-polystyrene resin; aminopropyl glass; isothiocyanato glass and others as purchased from Sigma company. All supports may be with or without a linker extending from the amino group on the support (eg. succinate linkage, amide, ether, alkyl chain with terminal carbon activated as free alcohol, bromide etc.).
Preparation of compound 2120
To a solution of 76 ( 1.0 equiv) was added methylene chloride (.1 M) and benzaldehyde ( 1.1 equiv), and the solution was exposed to ZnCI ( 1.1 equiv) at 25 °C and allowed to stir for 2.5 hour. The solution is then diluted with ether and then washed with a saturated solution of sodium bicarbonate (2X), water (2X), brine (1X) and then dried over MgSO4. The compound is purified by flash column chromatography to yield the desired
Figure imgf000155_0001
Figure imgf000156_0001
benzylidene and carried on as follows:
To a solution of benzylidene (1.0 equiv.) in THF (0.5 M) at 0 °C, is added NaH ( 1.0 equiv., 35% dispersion in mineral oil) over several portions. The reaction mixture is warmed to room temperature and stirred 1h. Next, the reaction is cooled to 0 °C and treated with benzyl bromide ( 1.0 equiv.) and stirred for 1.5 h. A saturated solution of ammonium chloride (50 mL) is then added dropwise to quench the reaction mixture at 0 °C and the mixture was diluted with ethyl acetate, washed with water (2X), brine (1X), dried over MgSO4 and evaporated. Purification by flash column chromatography yields tribenzyl ether 2120.
Preparation of compound 2130
Procedure adopted from Johansson R.; Samuelsson; B. J. Chem. Soc , Chem. Commun. , 1 984 , 201. To a solution of the benzylidene acetal 2120 ( 1 equi v ) and sodi u m cyanoborohydride (5 equiv.) in DMF ( .125 M) containing powedered 3 angtrsom molecular sieves is added trifluoroacetic acid ( 10 equiv) and the reaction is allowed to stir at 0 °C until no starting material remains. Reaction mixture is then diluted with ethyl acetate (2L) and washed with a small amount of water (2X) and brine (2X). Aqueous layer is back extracted with ethyl acetate (3X) and then recombined with the organic layer which was then dried over MgSO4 and evaporated. Purification by flash column chromatography yields the desired benzyl ether 2130.
Preparation of compound 2140
To a solution of 2130 ( 1.0 equivalents) in methylene chloride
(.10 Molar), is added triethylamine ( 1.1 equivalents) at 0 °C. Subsequent addition of tertbutyldiphenylsilylchloride ( 1 . 1 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X), brine ( 1X) and then dried (MgSO4) and concentrated. Purification by flash column chromatography affords the TBDPS ether which is subsequently carried on as follows:
To a solution of TBDPS ether ( 1.0 equiv.) in THF (0.5 M) at 0 °C, is added NaH ( 1.0 equiv., 35% dispersion in mineral oil) over several portions. The reaction mixture is warmed to room temperature and stirred l h. Next, the reaction is cooled to 0 °C and treated with benzoyl bromide to afford benzoate formation ( 1.0 equiv.) and stirred for 1.5 h. A saturated solution of ammonium chloride (50 mL) is then added dropwise to quench the reaction mixture at 0 °C and the mixture was diluted with ethyl acetate, washed with water (2X), brine ( 1X), dried over M g S O 4 and evaporated. Purification by flash column chromatography yields tribenzyl ether 2140. Preparation of compound 2150
The compound 2140 is then treated with tetrabutylammonium fluoride (2.0 equivalents) in THF (.1 Molar) and allowed to stir for an additional 2 hours at 25 °C. A saturated solution of ammonium chloride (50 mL) is then added dropwise to quench the reaction mixture at 0 °C and the mixture was diluted with ethyl acetate, washed with water (2X), brine ( 1X), dried over M g S O 4 and evaporated. Purification by flash column chromatography yields tribenzyl ether 2150.
Preparation of compound 2160
To a solution of 2150 ( 1.0 equivalents) in methylene chloride (.10 M), is added diisopropylethylamine (4.0 equivalents) at 25°C. The reaction is stirred for 5 minutes and then 2-cyanoethyl-
N, N-diisopropyl-chlorophosphoramidite ( 1.5 equiv) is added, as prepared from the procedures of Sinha et al. Nucl. Acids Res. 1984 , 12, 4539. After 15 minutes the reaction is complete and is next diluted with ether and next washed with brine ( 1X) and is then dried ( MgSO4 ) and concentrated. Purification by flash column chromatography (silica, 30% ethyl acetate in petroleum ether) affords compound 2160 (as shown in scheme 2004). Preparation of compound 2170
To a solution of alcohol 2150 (1.0 equiv.) in THF (0.5 M) at 0 °C, is added NaH (1.0 equiv., 35% dispersion in mineral oil) over several portions. The reaction mixture is warmed to room temperature and stirred 1h. Next, the reaction is cooled to 0 °C and exposed to the solid support functionalized with a bromide linker or any reasonable leaving group attached (1.0 equiv.)
Figure imgf000161_0001
and stirred for 2 hours. A saturated solution of ammonium chloride (50 mL) is added dropwise to quench the reaction mixture at 0 °C and the support was washed with ethyl acetate, 1 % NaOH in methanol (2X) to remove the benzoate and finally brine (IX) to give 2170. The solid support used is the standard
N- (2-Aminoethyl)-3-amino-propyl glass support; amino-polystyrene resin; aminopropyl glass; isothiocyanato glass and others as purchased from Sigma company. All supports may be with or without a linker extending from the amino group on the support (eg. succinate linkage, amide, ether, alkyl chain with terminal carbon activated as free alcohol, bromide etc.).
Prepartion of compound 3010
Procedure as described in Methods in Carbohydrate chemistry, Whistler, R., II, 1963 , p. 327. A mixture of 80 g anhydrous D-glucosamine hydrochloride or D-galactosamine hydrochloride from Aldrich chemical company, in 200 mL. methanol and 20g Dowex 50 (H+) acidic resin, is stirred at the boiling point in a round bottom flask. After 24-hr. reaction time, the resin is removed by filtration and ished three times with 20 ml. of methanol. The filrate and washings are combined and concentrated to about 125 ml by rotovap. The concentrate is allowed to cool to room temperature and the product crystallizes overnight and carried on as follows: The methyl glycoside is dissolved in chloroform (.5 M) and to it, is added phthalic anhydride ( 1.5 equiv.) and the reaction mixture is allowed to reflux at 70 °C for 4 h. The product
Figure imgf000164_0001
3010 is then crystallized and carried onto the next step.
Preparation of compound 3020
To a solution of alcohol 3010 ( 1.0 equiv.) in THF (0.5 M) at 0 °C, is added NaH (3.3equiv., 35% dispersion in mineral oil) over several portions. The reaction mixture is warmed to room temperature and stirred 1h. Next, the reaction is cooled to 0 °C and treated with benzyl bromide (3.3 equiv.) and stirred for 1.5 h. A saturated solution of ammonium chloride (50 mL) is then added dropwise to quench the reaction mixture at 0 °C and the mixture was diluted with ethyl acetate, washed with water (2X), brine ( IX), dried over MgSO4 and evaporated. Purification by flash column chromatography yields tribenzyl ether and is carried on as follows:
To a solution ol tribenzyl ether in nitromethane is added trimethylsilyl cyanide (3.0 equivalents) and then SnCl4 ( .02 equivalents ). The mixture is stirred for one hour and then an aqueous solution ot sodium acetate was added to hydrolyze the remaining trimethylsilyl cyanide. The mixture is evaporated and the remaining oil is resuspended in dichloromethane and washed with sodium acetate solution ( 1X), water ( 1X), brine ( 1X) and then dried over magnesium sulphate and concentrated. The crude solid is then recrystallized from methanol is next dissolved in ethanol ( 0.15 M) and then concentrated H2S O4 (0.01 equivalents-catalytic) is added. The reaction mixture is heated to 85 °C for eight hours. The solution is next concentrated in vacuo and purification by flash column chromatography affords compound 3020 scheme 3000.
Prepartion of compound 3030
To a solution of 3020 ( 1.0 equivalents) in methylene chloride (.10 Molar), is added potassium carbonate (2.0 equivalents) at 0°C. Subsequent addition of 9-fluorenylmethyl chloroformate (FMOC-Cl, 1.2 equivalents) is followed by stirring for 2 hours and then the reaction is diluted with diethylether and washed with ammonium chloride (2X), brine ( 1X) and then dried ( M g S O 4) and concentrated. Purification by flash column chromatography affords product which is carried on as follows:
To a solution of ester in ethanol (.13 Molar), is added sodium ethoxide (0.3 equivalents) and the reaction mixture is stirred for two hours at room temperature . The solution is then concentrated in vacuo and purification by flash column chromatography affords compound 3030 scheme 3000.
Preparation of compound 3040
To a stirred solution of the acid 3030 ( 1.0 equivalents) and the ( 1.1 equivalents) in dimethylformamide (.10 Molar) at 25 °C, is added 1 -hydroxybenzotriazole (HOBT; 1 .1 equivalents). N e x t dicyclohexylcarbodiimide ( 1.2 equivalents) is added and the reaction is stirred for 2 hours. The mixture is then exposed to the solid support and mixed for 24 hours. (The solid support used is the standard N-(2-Aminoethyl)-3-amino-propyl glass support; amino-polystyrene resin; aminopropyl glass; isothiocyanato glass and others as purchased from Sigma company. All supports may be with or without a linker extending from the amino group on the support (eg. succinate linkage, amide, ether, alkyl chain with terminal carbon activated as free alcohol, bromide etc.)). The mixture is then
diluted with ether, washed with aqueous NaHCO3 (2X), water (2X), and brine (2X). Next, the compound/support ( 1.0 equivalents) in dimethyl-formamide (.10 Molar) at 25 °C, is added piperidine ( 1.1 equivalents). The support is stirred or exposed for 1 hour and is then diluted with ether, and washed with aqueous CuSO4 (2X), water (2X), and brine (2X). The final step affords compound 3040.
Figure imgf000168_0001
Physical Data for scheme 9.
Phosphoramidate 138 (2 diastereomers): IR, (neat) cm-1; 3089, 2964, 2927, 2856, 2253, 1497, 1455, 1396, 1363, 1253, 1184, 1156, 1094, 1028, 978, 876, 836, 779, 735,
1H-NMR (400 MHz, C6D6) : 57.34 (m, 5 H, Ph), 7.14 (m, 10 H, Ph), 4.97 (m, 4 H, CH2Ph) , 4.78 (m, 2 H, CH2Ph) , 4.07-3.24 (m, 13 H, OCH, OCH2, CH2CN) , 1.81 (m, 2 H, CH(CH3)2), 1.16 (m, 12 H, CH3CH) , 1.03, 1.02 (2 s, 9 H, iBuSi), 0.20, 0.18, 0.16, 0.15, (4 s, 6 H, Me2Si);
HRMS: C43H63O7N2PSi, Calc . (M+Cs+): 911.3197; found:
911.3185.
Naphthoylester 136 IR, (neat) cm-1 : 3494, 3062, 2919, 1716, 1630, 1600, 1454, 1355, 1284, 1228, 1197, 1091,
779, 736; 1H-NMR (250 MHz, CDCI3): δ 8.58 (s, 1 H, Ar), 8.00 (m, 2 H, Ar), 7.89 (m, 2 H, Ar), 7.59 (m, 2 H, Ar), 7.32 (m, 15 H, Ph), 4.95 (m, 3 H) , 4.90 (d, J=4.5 Hz, 1 H) , 4.69 (m, 3 H), 4.52 (dd, J = 3.9, 12.0 Hz, 1 H) , 3.91 (dd J - 2.6, 12.0, 1 H) , 3.83 (d, J =
8.3, 1 H), 3.70 (m, 4 H), 3.96 (m, 1 H) , 2.25 (s, 1 H, OH). HRMS: C39H38O7 Calc. (M+Cs+) : 751.1672; found: 751.1668. Dimer 142 IR, (neat) cm-1. 3397, 3030, 2923, 2254, 1718,
1653, 1629, 1497, 1453, 1355, 1284, 1227, 1197, 1094, 1029, 780. 1H-NMR (400 MHz, C6D6) : δ 8.82 (s, 1 H, Ar), 8.26 (d, 1 H, Ar), 7.72 (m, 1 H, Ar), 7.61 (m, 1 H, Ar), 7.48 (m, 1 H, Ar), 7.37-6.95 (m, 32 H, Ar, Ph), 4.89-4.18 (m, 21 H, CH2Ph, CH2-Ar, -CH2CH2CN, CHCH2-Ar and CH2OH) , 3.95-3.45 (m, 13 H, CH- and CH2 - sugar), 1.71 (s, 1 H, OH); HRMS: C170H72O5NP calc. (M+H+):
1198.4718; found: 1198.4715. Tetramer iso IR, (neat) cm-1; 3420, 3064, 2924, 2255, 1721, 1497, 1455, 1357, 1278, 1028, 737. 1H-NMR (400 MHz, CDCI3) : δ 8.41 (s, 1 H, Ar), 8.00 (m, 2 H, Ar), 7.91 (m, 2 H, Ar), 7.55 (m, 2 H, Ar), 7.30 (m, 60 H, Ph), 4.93-4.05 (m, 39 H, CH2Ph, CH2-Ar, CH2CH2CN and CH2OH) , 3.88-3.27 (m, 23 H, CH- and CH2- sugar ) , 2.58 (s, 1 H, OH). HRMS: C132H140O31N3P3 Calc. (M+Cs+) :
2488.7738; found: 2488.7758.
Tetramer 154 IR, (neat) cm-1: 3376, 2934, 1450, 1244, 1110, 1088. 1H-NMR (400 MHz, D2O): δ 8.41 (s, 1 H, Ar), 8.00 (m, 2 H, Ar), 7.91 (m, 2 H, Ar), 7.55 (m, 2 H, Ar), 4.93-4.05 (m, 4 H, CH2-Ar and CH2OH) , 3.88-3.27 (m, 32 H, CH- and CH2 - sugar ) ; HRMS: C39H59O31P3 Calc.
(M+H+) : 1117.2331; found: 1117.2350.

Claims

What is claimed is:
1. An oligomeric carbopeptoid compound comprising carbohydrate amino acid subunits (CA's) coupled to one another via an amide linkage having a carbonyl carbon and an amido nitrogen represented by the following formula:
CA1-(CO-NH)-CA2
wherein:
CA1 is a first carbohydrate amino acid subunit having an anomeric carbon bonded to the carbonyl carbon of said amide linkage for forming a C-glycosidic linkage therewith and
CA2 is a second carbohydrate amino acid subunit having a non-anomeric carbon bonded to the amido nitrogen of said amide linkage.
2. In a process for synthesizing an oligomeric carbopeptoid compound, a coupling step wherein two or more carbohydrate amino acid subunits (CA's) are coupled by means of an amide linkage having a carbonyl carbon and an amido nitrogen for synthesizing said oligomeric carbopeptoid compound, said amide linkage being represented by a formula as follows:
CA1-(CO-NH)-CA2
wherein:
CA1 is a first carbohydrate amino acid subunit having an anomeric carbon bonded to the carbonyl carbon of said amide linkage for forming a C-glycosidic linkage therewith; and
CA2 is a second carbohydrate amino acid subunit having a non-anomeric carbon bonded to the amido nitrogen of said amide linkage.
3 . A library of oligomeric carbopeptoid compounds employable for drug screening , each ol igomeric carbopeptoid compound including at l eas t two carbohydrate amino acid subunits (CA ' s ) coupled to one another via an amide linkage having a carbonyl carbon and an amido nitrogen , said amide linkage being represented by the following formula :
CA1- (CO-NH) -CA2
wherein :
CA1 is a first carbohydrate amino acid subunit having an anomeric carbon bonded to the carbonyl carbon of said amide linkage for forming a C-glycosidic linkage therewith; and
CA2 is a second carbohydrate amino acid subunit having a non-anomeric carbon bonded to the amido nitrogen of said amide linkage.
4. An improved process for synthesizing a library of oligomers, the process employing an elongation step wherein subunits are coupled to one another to produce the oligomers, wherein the improvement comprises:
in said elongation step the oligomer includes at least two carbohydrate amino acid subunits (CA's) coupled to one another via an amide linkage having a carbonyl carbon and an amido nitrogen represented by the following formula:
CA1-(CO-NH)-CA2
wherein:
CA1 is a first carbohydrate amino acid subunit having an anomeric carbon bonded to the carbonyl carbon of said amide linkage for forming a C-glycosidic linkage therewith; and CA2 is a second carbohydrate amino acid subunit having a non-anomeric carbon bonded to the amido nitrogen of said amide linkage.
5. A derived carbohydrate amino acid having an anomeric carbon and non-anomeric carbons,
said anomeric carbon being substituted with a carboxyl radical,
each of said non-anomeric carbons being substituted with a radical selected from the group consisting of blocked hydroxyl, blocked amino, differentially protected amino, and hydrogen, with the proviso that at least one radical is a differentially protected amino.
6. A derived carbohydrate amino acid having an anomeric carbon and non-anomeric carbons,
said anomeric carbon being substituted with a carboxyl radical,
each of said non-anomeric carbons being substituted with a radical selected from the group consisting of blocked hydroxyl, blocked amino, unprotected amino, and hydrogen, with the proviso that at least one radical is an unprotected amino and at least one radical is a blocked hydroxyl or amino.
7. An oligomeric carbonucleotoid molecule comprising carbohydrate C-glycoside subunits (CG's) coupled to one another via a phosphodiester linkage represented by the following structure:
CG1-C1'-(O-PO(OH)-O)-CG2 wherein:
(O-PO(OH)-O) is said phosphodiester linkage; CG1-C1' is a first carbohydrate C-glycoside subunit having an anomeric carbon forming a C-glycosidic bond with a carbon C1', said carbon C1' being bonded to said phosphodiester linkage; and
CG2 is a second carbohydrate C-glycoside subunit having a non-anomeric carbon bonded to said phosphodiester linkage.
8. In a process for synthesizing an oligomeric carbonucleotoid molecule, a coupling step wherein two or more carbohydrate C-glycoside subunits (CG's) are coupled by means of a phosphodiester linkage, said phosphodiester linkage being represented by a formula as follows:
CG1-C1'-(O-PO(OH)-O)-CG2 wherein:
(O-PO(OH)-O) is said phosphodiester linkage;
CG1-C1' is a first carbohydrate C-glycoside subunit having an anomeric carbon forming a C-glycosidic bond with a carbon C1', said carbon C1' being bonded to said phosphodiester linkage; and
CG2 is a second carbohydrate C-glycoside subunit having a non-anomeric carbon bonded to said phosphodiester linkage.
9. A library of oligomeric carbonucleotoid molecules employable for drug screening, each oligomeric carbonucleotoid molecule including at least two carbohydrate
C-glycoside subunits (CG's) coupled to one another by means of a phosphodiester linkage, said phosphodiester linkage being represented by a formula as follows:
CG1-C1'-(O-PO(OH)-O)-CG2 wherein :
(O-PO(OH)-O) is said phosphodiester linkage;
CG1-C1' is a first carbohydrate C-glycoside subunit having an anomeric carbon forming a C-glycosidic bond with a carbon C1', said carbon C1' being bonded t'o said phosphodiester linkage; and
CG2 is a second carbohydrate C-glycoside subunit having a non-anomeric carbon bonded to said phosphodiester linkage.
10. An improved process for synthesizing a library of oligomers, the process employing an elongation step wherein subunits are coupled to one another to produce the oligomers, wherein the improvement comprises:
in said elongation step the oligomer is a carbonucleotoid including at least two carbohydrate C-glycoside subunits (CG's) are coupled by means of a phosphodiester linkage, said phosphodiester linkage being represented by a formula as follows:
CG1-C1'-(O-PO(OH)-O)-CG2 wherein:
(O-PO(OH)-O) is said phosphodiester linkage;
CG1-C1' is a first carbohydrate C-glycoside subunit having an anomeric carbon forming a C-glycosidic bond with a carbon C1', said carbon C1' being bonded to said phosphodiester linkage; and
CG2 is a second carbohydrate C-glycoside subunit having a non-anomeric carbon bonded to said phosphodiester linkage.
11. A derived carbohydrate C-glycoside having an anomeric carbon and non-anomeric carbons,
said anomeric carbon forming a C-glycosidic bond with a carbon C1', said carbon C1' being bonded to an activated phosphite,
each of said non-anomeric carbons being substituted with a radical selected from the group consisting of blocked hydroxyl, differentially protected hydroxyl, and hydrogen, with the proviso that at least one radical is a differentially protected hydroxyl.
12. A derived carbohydrate C-glycoside having an anomeric carbon and non-anomeric carbons,
said anomeric carbon forming a C-glycosidic bond with a carbon C1', said carbon C1' being bonded to an activated phosphite,
each of said non-anomeric carbons being substituted with a radical selected from the group consisting of blocked hydroxyl, unprotected hydroxyl, and hydrogen, with the proviso that at least one radical is an unprotected hydroxyl and at least one radical is a blocked hydroxyl.
PCT/US1996/003227 1995-03-08 1996-03-08 Carbopeptoids and carbonucleotoids WO1996027379A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP96908737A EP0827406A1 (en) 1995-03-08 1996-03-08 Carbopeptoids and carbonucleotoids
AU51882/96A AU717099B2 (en) 1995-03-08 1996-03-08 Carbopeptoids and carbonucleotoids
US08/913,035 US6204376B1 (en) 1996-03-08 1996-03-08 Carbopeptoids and carbonucleotoids
US10/140,597 US20030013870A1 (en) 1996-03-08 2002-05-07 Carbopeptoids and carbonucleotoids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US40103995A 1995-03-08 1995-03-08
US08/401,039 1995-03-08

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US09/417,877 Division US6384211B1 (en) 1996-03-08 1999-10-13 Carbopeptoids and carbo-nucleotoids

Publications (2)

Publication Number Publication Date
WO1996027379A1 true WO1996027379A1 (en) 1996-09-12
WO1996027379A9 WO1996027379A9 (en) 1996-11-28

Family

ID=23586019

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/003227 WO1996027379A1 (en) 1995-03-08 1996-03-08 Carbopeptoids and carbonucleotoids

Country Status (4)

Country Link
EP (1) EP0827406A1 (en)
AU (1) AU717099B2 (en)
CA (1) CA2214789A1 (en)
WO (1) WO1996027379A1 (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5756712A (en) * 1997-01-23 1998-05-26 E. I. Du Pont De Nemours And Company Peptidodisaccharides as oligosaccharide mimetics
US5919967A (en) * 1997-04-11 1999-07-06 Epix Medical, Inc. Process for synthesizing phosphodiesters
US6251433B1 (en) 1996-08-13 2001-06-26 Chiron Corporation Polycationic polymers
US6569450B1 (en) 1997-08-13 2003-05-27 Chiron Corporation Lipid-conjugated polyamide compounds and related compositions and methods thereof
CN110526950A (en) * 2019-09-23 2019-12-03 济南山目生物医药科技有限公司 A kind of preparation method of five-O- acetylmannosamine sugar of alpha-
US20230045939A1 (en) * 2018-11-22 2023-02-16 Idorsia Pharmaceuticals Ltd Stable vaccine against clostridium difficile

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5212298A (en) * 1989-08-16 1993-05-18 Monsanto Company Method for producing synthetic N-linked glycoconjugates

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5573905A (en) * 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
WO1995003315A2 (en) * 1993-07-21 1995-02-02 Oxford Glycosystems Ltd Saccharides, their synthesis and use
WO1995018971A1 (en) * 1994-01-11 1995-07-13 Affymax Technologies N.V. Methods for the solid phase synthesis of glycoconjugates

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5212298A (en) * 1989-08-16 1993-05-18 Monsanto Company Method for producing synthetic N-linked glycoconjugates

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANGEW. CHEM. INT. ED. ENGL., 1993, Vol. 32, No. 4, KESSLER HORST, "Peptoids - A New Approach to the Development of Pharmaceuticals", pages 543-544. *
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, Vol. 114, ZUCKERMAN et al., "Efficient Method for the Preparation of Peptoids [Oligo(N-Substituted Glycines)] by Submonomer Solid-Phase Synthesis", pages 10646-10647. *
PROC. NATL. ACAD. SCI. U.S.A., October 1992, Vol. 89, SIMON et al., "Peptoids: A Modular Approach to Drug Discovery", pages 9367-9371. *
See also references of EP0827406A4 *

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6251433B1 (en) 1996-08-13 2001-06-26 Chiron Corporation Polycationic polymers
US6468986B1 (en) 1996-08-13 2002-10-22 Chiron Corporation Compositions and methods for polynucleotide delivery
US7462592B2 (en) 1996-08-13 2008-12-09 Novartis Vaccines And Diagnostics, Inc. Compositions and methods for polynucleotide delivery
US5756712A (en) * 1997-01-23 1998-05-26 E. I. Du Pont De Nemours And Company Peptidodisaccharides as oligosaccharide mimetics
US5919967A (en) * 1997-04-11 1999-07-06 Epix Medical, Inc. Process for synthesizing phosphodiesters
US6569450B1 (en) 1997-08-13 2003-05-27 Chiron Corporation Lipid-conjugated polyamide compounds and related compositions and methods thereof
US6572881B1 (en) 1997-08-13 2003-06-03 Chiron Corporation Lipid-conjugated polyamide compounds and related compositions and methods thereof
US7214384B2 (en) 1997-08-13 2007-05-08 Novartis Vaccines And Diagnostics, Inc. Lipid-conjugated polyamide compounds
US20230045939A1 (en) * 2018-11-22 2023-02-16 Idorsia Pharmaceuticals Ltd Stable vaccine against clostridium difficile
US12187756B2 (en) * 2018-11-22 2025-01-07 Idorsia Pharmaceuticals Ltd Stable vaccine against Clostridium difficile
CN110526950A (en) * 2019-09-23 2019-12-03 济南山目生物医药科技有限公司 A kind of preparation method of five-O- acetylmannosamine sugar of alpha-
CN110526950B (en) * 2019-09-23 2023-08-04 济南山目生物医药科技有限公司 Preparation method of alpha-five-O-acetyl mannose

Also Published As

Publication number Publication date
AU717099B2 (en) 2000-03-16
EP0827406A4 (en) 1998-03-11
EP0827406A1 (en) 1998-03-11
AU5188296A (en) 1996-09-23
CA2214789A1 (en) 1996-09-12

Similar Documents

Publication Publication Date Title
AU606909B2 (en) Sialic acid derivatives having active carbonyl group
CN113527388B (en) Method for stereoselective synthesis of beta-2-deoxy sugar, 2-deoxy-2-azido sugar and glucosidic bond
WO2019113889A1 (en) Preparation method for helicobacter pylori lipopolysaccharide outer core octasaccharide
Knapp et al. Reactions of some pyranoside diol monotriflates with nucleophiles and bases
WO1996027379A1 (en) Carbopeptoids and carbonucleotoids
WO1996027379A9 (en) Carbopeptoids and carbonucleotoids
US6204376B1 (en) Carbopeptoids and carbonucleotoids
EP0828729A1 (en) Collection of activated glycoside compounds and their biological use
EP1144430A1 (en) Protecting groups for carbohydrate synthesis
JP5238822B2 (en) Process for synthesizing docetaxel, its intermediate and its synthesis
CN114874345A (en) Chemical synthesis method of helicobacter pylori core lipopolysaccharide oligosaccharide antigen sugar chain
CA1300532C (en) Covalent oligonucleotide-horseradish peroxidase conjugate
CN115785168B (en) Method for preparing 4-demethoxydaunorubicin hydrochloride
US5371202A (en) Processes of preparing sialoglycosyl compounds
JP4555466B2 (en) Substituted tetrahydropyran derivatives, processes for their preparation, their use as medicaments or diagnostics and medicaments containing them
CN104693252A (en) Glycosidic taxane compound and preparation method thereof
CN1172945C (en) A New Synthesis Method of Antineoplastic Drug Etoposide
EP0852231A2 (en) 16-Membered macrolide derivative having sustained antibacterial activity in plasma, synthesis intermediate thereof and process for producing the same
Nishida et al. Syntheses and 1H-NMR Studies of Methyl 4, 6-Di-O-glucopyranosyl-β-d-glucopyranosides Chirally Deuterated at the (l→ 6)-Linkage Moiety
Johnson et al. An efficient synthesis of 6, 6′-DI-O-acylated α, α-trehaloses
CN110041377B (en) A kind of synthetic method of O-mannan core structure
Ledvina et al. New Effective Synthesis of (N-Acetyl-and N-Stearoyl-2-amino-2-deoxy-β-D-glucopyranosyl)-(1→ 4)-N-acetylnormuramoyl-L-2-aminobutanoyl-D-isoglutamine, Analogs of GMDP with Immunopotentiating Activity
US7456309B2 (en) Reusable universal polymer support for repetitive oligonucleotide synthesis
Ogawa et al. An efficient synthesis of sulfo Lewis X analog containing 1-deoxynojirimycin
WO2003095478A1 (en) Thermostable and monoconjugatable gold cluster complexes

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AL AM AT AU AZ BB BG BR BY CA CH CN CZ DE DK EE ES FI GB GE HU IS JP KE KG KP KR KZ LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK TJ TM TR TT UA UG US UZ VN AM AZ BY KG KZ MD RU TJ TM

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): KE LS MW SD SZ UG AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN

COP Corrected version of pamphlet

Free format text: PAGES 43-168,DESCRIPTION,REPLACED BY NEW PAGES 43-167;PAGES 169-174,CLAIMS,REPLACED BY NEW PAGES 168-173;DUE TO LATE TRANSMITTAL BY THE RECEIVING OFFICE

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
ENP Entry into the national phase

Ref document number: 2214789

Country of ref document: CA

Ref country code: CA

Ref document number: 2214789

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 1996908737

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 08913035

Country of ref document: US

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWP Wipo information: published in national office

Ref document number: 1996908737

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1996908737

Country of ref document: EP