WO1996017927A3 - Reporter cell line - Google Patents

Reporter cell line Download PDF

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Publication number
WO1996017927A3
WO1996017927A3 PCT/EP1995/004819 EP9504819W WO9617927A3 WO 1996017927 A3 WO1996017927 A3 WO 1996017927A3 EP 9504819 W EP9504819 W EP 9504819W WO 9617927 A3 WO9617927 A3 WO 9617927A3
Authority
WO
WIPO (PCT)
Prior art keywords
nfλb
expression
cell line
gene
gene product
Prior art date
Application number
PCT/EP1995/004819
Other languages
French (fr)
Other versions
WO1996017927A2 (en
Inventor
Stefan Nilsson
Original Assignee
Karobio Ab
Stefan Nilsson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Karobio Ab, Stefan Nilsson filed Critical Karobio Ab
Priority to AU47134/96A priority Critical patent/AU4713496A/en
Publication of WO1996017927A2 publication Critical patent/WO1996017927A2/en
Publication of WO1996017927A3 publication Critical patent/WO1996017927A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides a cell line including a first reporter gene arranged to express an assayable first gene product, expression of the first reporter gene being AP-1 mediated, and a second reporter gene arranged to express a second assayable gene product, expression of the second reporter gene being mediated by NFλB. Thus the present invention provides a convenient one cell line-based assay system which can be used to rapidly test a large number of compounds for potential anti-inflammatory activity. According to a further aspect of the invention there is provided a method of testing a compound for both AP1 and NFλB inhibitory activity, the method comprising providing cells in accordance with the first aspect of the invention, stimulating AP1 and NFλB activity in the cells and contacting the cells with the compound to be tested and monitoring expression of the first and second gene products wherein inhibition of AP1 expression of the first gene product, and inhibition of NFλB mediated expression of the second gene product is indicative that the compound has both AP1 and NFλB-inhibitory activity.
PCT/EP1995/004819 1994-12-05 1995-12-05 Reporter cell line WO1996017927A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU47134/96A AU4713496A (en) 1994-12-05 1995-12-05 Reporter cell line

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9424497A GB9424497D0 (en) 1994-12-05 1994-12-05 Reporter cell line
GB9424497.7 1994-12-05

Publications (2)

Publication Number Publication Date
WO1996017927A2 WO1996017927A2 (en) 1996-06-13
WO1996017927A3 true WO1996017927A3 (en) 1996-08-29

Family

ID=10765423

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1995/004819 WO1996017927A2 (en) 1994-12-05 1995-12-05 Reporter cell line

Country Status (3)

Country Link
AU (1) AU4713496A (en)
GB (1) GB9424497D0 (en)
WO (1) WO1996017927A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1157126A4 (en) * 1999-02-24 2005-03-02 Gen Hospital Corp Method for cloning signal transduction intermediates
WO2003048202A2 (en) * 2001-12-03 2003-06-12 Asahi Kasei Pharma Corporation Nf-kappab activating genes

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992007072A1 (en) * 1990-10-10 1992-04-30 La Jolla Cancer Research Foundation Method of inhibiting transcription utilizing nuclear receptors
WO1994017208A1 (en) * 1993-01-21 1994-08-04 President And Fellows Of Harvard College Methods and diagnostic kits utilizing mammalian stress promoters to determine toxicity of a compound
DE4311835A1 (en) * 1993-04-07 1994-10-13 Boehringer Ingelheim Int Method for inhibiting gene transcription
WO1995006754A1 (en) * 1993-09-01 1995-03-09 The Regents Of The University Of California Methods for screening antiestrogen compounds
WO1995031722A1 (en) * 1994-05-18 1995-11-23 Ligand Pharmaceuticals, Inc. Screening for cytokine modulators

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992007072A1 (en) * 1990-10-10 1992-04-30 La Jolla Cancer Research Foundation Method of inhibiting transcription utilizing nuclear receptors
WO1994017208A1 (en) * 1993-01-21 1994-08-04 President And Fellows Of Harvard College Methods and diagnostic kits utilizing mammalian stress promoters to determine toxicity of a compound
DE4311835A1 (en) * 1993-04-07 1994-10-13 Boehringer Ingelheim Int Method for inhibiting gene transcription
WO1995006754A1 (en) * 1993-09-01 1995-03-09 The Regents Of The University Of California Methods for screening antiestrogen compounds
WO1995031722A1 (en) * 1994-05-18 1995-11-23 Ligand Pharmaceuticals, Inc. Screening for cytokine modulators

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
A. RAY AND K.E. PREFONTAINE: "Physical association and functional antagonism between the p65 subunit of transcription factor NF-kappaB and the glucocorticoid receptor", PROC. NATL.ACAD SCI., vol. 91, January 1994 (1994-01-01), NATL. ACAD SCI.,WASHINGTON,DC,US;, pages 752 - 756, XP002004799 *
ALAM J ET AL: "REPORTER GENES: APPLICATION TO THA STUDY OF MAMMALIAN GENE TRANSCRIPTION", ANALYTICAL BIOCHEMISTRY, vol. 188, no. 2, 1 August 1990 (1990-08-01), pages 245 - 254, XP000563914 *
C. JONAT ET AL.: "Antitumor promotion and antiinflammation: Down-modulation of AP-1(Fos/Jun) activity by glucocorticoid hormone", CELL, vol. 62, 21 September 1990 (1990-09-21), CELL PRESS,CAMBRIDGE,MA,US;, pages 1189 - 1204, XP002004798 *

Also Published As

Publication number Publication date
WO1996017927A2 (en) 1996-06-13
GB9424497D0 (en) 1995-01-25
AU4713496A (en) 1996-06-26

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