WO1996011707A1 - Renibacterium salmoninarum vaccine and method for its preparation - Google Patents

Abstract

A vaccine and method for treating fish susceptible infection by Renibacterium salmoninarum is described. The vaccine comprises killed microorganisms that lack intact cell-surface-associated protein p57. The vaccine can be used in combination with additional materials, such as, without limitation, adjuvants, plasticizers, pharmaceutical excipients, antigens other than the cells lacking intact cell-surface-associated protein p57, diluents, carriers, binders, lubricants, glidants, aesthetic compounds, such as flavoring and colouring agents, and combinations thereof. The vaccine may be enteric-coated for oral delivery. The enteric coating generally comprises a polymer coating that is impervious to dissolution and/or degradatation in the stomach, but is dissolved upon passing to the higher pH environments of the intestine. A preferred embodiment of the vaccine is made using spherical sugar microspheres. The microsphere is coated with a first layer comprising the killed Renibacterium salmoninarum microorganisms lacking intact cell-surface-associated protein p57. The sugar microsphere is then coated with a second enteric-coating layer comprising a material that is impervious to dissolution and/or degradataion in the stomach of the fish.

Description

RENIBACTERIUM SALMONINARUM VACCINE AND METHOD FOR ITS PREPARATION

FIELD OF THE INVENTION

This invention concerns a vaccine and method for treating fish susceptible to infection by Renibacterium salmoninarum .

ACKNOWLEDGEMENT OF GOVERNMENT SUPPORT This invention was made with Government support under USCS-CSRS #92-34123-7665 and USDA (WRAC) #91-38500-6078. The Government may have certain rights in the invention. BACKGROUND OF THE INVENTION

Bacterial kidney disease (BKD) results from infection by Renibacterium salmoninarum . BKD is a chronic and systemic disease that generally leads to mortality in juvenile and adult salmonids, both in fresh water and marine environments. Bacterial Kidnev Disease of Salmonid Fish. Annu. Rev. Microbiol, 35:273-298. Salmonids are fish of the family Salmonidae , which are soft-finned fishes such as salmon, trout, chars and whitefishes.

Renibacterium salmoninarum is a slow-growing gram-positive bacterium. The bacterium is endemic in wild anadromous (migrating up rivers from the sea to breed in fresh water) salmonid populations on both coasts of North America, and has been found in wild Atlantic salmon and sea trout. After infection, Renibacterium salmoninarum localizes in the kidney from which infection rapidly becomes systemic.

Farming of marine species is an ancient practice, and aqua culturing of fish has increased significantly over the last twenty years. Commercial aqua culturing requires maintaining high densities of cultured fish. This increases the likelihood of economic loss from diseases such as BKD relative to less-dense fish populations. Although the actual losses attributed to BKD have not yet been calculated, the disease is known to be one of the most important bacterial diseases affecting resident anadromous salmonid stocks in the Pacific Northwest. Because BKD is one of the most prevalent diseases of cultured salmonids, it has had a significant economic impact on the fishing and aqua culture industries.

There still are limited effective methods for controlling BKD despite its economic impact. One reason for this is that the bacteria is capable of adjusting to different conditions as an intracellular parasite, and has the ability to survive and multiply in phagocytic cells (cells that engulf and digest foreign bodies). Current approaches to managing BKD outbreaks include stress reduction, quarantine, chemotherapy (antibiotic treatment), total destruction of the infected population and complete sterilization of the facilities. These approaches to BKD infection are not commercially appealing, and are difficult to administer to large fish populations.

There are no known vaccines effective for treating fish susceptible to infection by BKD, despite the continuous efforts by those skilled in the art. For instance, McCarthy reported an attempt to vaccinate fish susceptible to BKD using two preparations of formalin- inactivated cells of Renibacterium salmoninarum . McCarthy et al .. Immunization of Rainbow Trout. Salmo eatrdneri Against Bacterial Kidnev Disease¹ Preliminary Efficacy Evaluation . J. of Fish Dis., 7:65-71 (1984). The bactenns were administered without adjuvant by IP-uyection, immersion, or two-step hyperosmotic infiltration. No significant protection was afforded by these methods.

Furthermore, Kaattaπ et al. have treated salmonids with a number of potential lmmunogens in an attempt to confer immunity to fish susceptible to BKD infection. These lrnmunogens included cell-wall fractions, fractured cells and extracellular products. Kaattaπ et al . , Development of a Vaccine for Bacterial Kidnev Disease . Bonneville Power Administration

Final Report, (1990). These immunogens were administered by lntrapentoneal injection, orally, and by immersion with and without adjuvant. None of these early preparations protected fish. In fact, some of these preparations exacerbated the disease.

The route of delivering vaccines often is an important factor for the successful vaccination of fish. lntrapentoneal vaccination is generally the most effective method for vaccinating any species, even though IP vaccination is labor intensive. Immersion is another vaccination method, which is widely used on smaller fish (fish that weigh less than about 10 to 15 grams). The standard immersion method involves exposing fish to the vaccine in aerated standing water for a minimum of 20 seconds. The disadvantage of immersion vaccination is that it is limited by the weight of fish that can be immunized per unit volume of vaccine. And, immersion vaccination usually provides lower levels of immunity than other techniques, due to the stress it causes fish.

SUMMARY OF THE INVENTION Based on the discussion provided above, it is apparent that a vaccine is needed for protecting salmonids against infection by Renibacterium salmoninarum . The present invention provides such a vaccine, as well as a method for treating fish using the vaccine The vaccine compπses killed Renibacterium salmoninarum microorganisms that are devoid of intact cell-surface-associated protein p57. Although vaccines can be made using virtually any strain of Renibacterium salmoninarum , operative vaccines have been made using Renibacterium salmoninarum microorganisms having the identifying characteristics of a microorganism selected from the group consisting of Renibacterium salmoninarum ATCC strain 33209 and Renibacterium salmoninarum D6 isolate The vaccine can be used in combination with additional materials, such as, without limitation, materials selected from the group consisting of ad_juvants, plasticizers, pharmaceutical excipients, antigens other than cells lacking mtact cell- surface-associated protein p57, diluents, earners, binders, lubneants, glidants, aesthetic compounds, such as flavonng and colonng agents, and combinations thereof

The vaccine also may be entenc-coated for oral delivery The entenc coating protects the vaccine from proteases and from the relatively low pH levels of the stomach. This allows the vaccine to reach the hindgut associated with lymphoid tissue, which maximizes the effectiveness of the vaccine for protecting fish. The enteric coating typically comprises a polymer coating that is unaffected by acidic pH, but which is dissolved upon passing to the higher pH environments of the pyloric caecum and intestine. The pH of salmonid stomachs varies from about 1.5 to about 4.8. The physiologic pH rapidly increases in the intestine of the fish to pH values of greater than about 5, and continues to increase to a pH of about 8 in the anus region of the fish. As a result, the polymer coating should not dissolve until in an environment where the pH is greater than about 5.0 and less than about 8. As a result, enteric-coating materials useful for the invention may be selected from the group consisting of enteric-coating materials, particularly polymeric materials, that dissolve in a liquid having a pH of from about 5 to about 8.

Oral administration is a generally preferred method of vaccinating fish against BKD using vaccines of the present invention. Oral vaccinations provide an ideal method for the mass administration of the vaccine to fish. Oral vaccination also is not limited by the size of the fish that can be handled, and it reduces the stress on the fish associated with immersion and IP vaccination. Furthermore, oral vaccines offer the additional advantage of stimulating mucosal immunity.

A preferred embodiment of the vaccine is made by coating spherical sugar microspheres (beads) with vaccine formulations. The beads can be virtually any material, now known or hereinafter developed, that is useful for delivering pharmacological materials. By way of example and without limitation, dextrose beads have been shown to be useful for forming such beads. The beads generally have a mesh size of from about 10 to about 60 mesh, preferably from about 20 to about 35 mesh, and even more preferably from about 25 to about 30 mesh. The beads generally are coated with a first layer comprising the killed

Renibacterium salmoninarum microorganisms lacking intact cell-surface-associated protein p57. This coating also may comprise additional materials, such as materials selected from the group consisting of adjuvants, plasticizers, pharmaceutical excipients, antigens other than cells lacking intact cell-surface-associated protein p57, diluents, carriers, binders, lubricants, glidants, aesthetic compounds, such as flavoring and coloring agents, and combinations thereof. For instance, a disintegrant or a super disintegrant often is used to help disperse the material once it is ingested. One example of a super disintegrant is sodium starch glycolate.

The bead is then coated with a second coating layer comprising an enteric-coating layer. This layer generally is a polymeric layer wherein the polymer is impervious to dissolution and/or degradation in the stomach of the fish, but does dissolve upon passing out of the stomach. That is, the polymer generally is impervious to dissolution in an aqueous media having a low pH, such as a pH of less than about 5, but is dissolved by an aqueous media having a pH value of from about 5 to about 8. There are numerous materials that are potentially useful for coating the beads as discussed in detail below. Solely by way of example, a polymenc mateπal currently known to be suitable for coating the beads is poly(methylacryhc acid-ethyl acrylate).

A preferred embodiment of the second bead coating compnses a mixture that includes about 2 weight percent to about 50 weight percent poly(methylacrylιc acid-ethyl acrylate), less than about 50 weight percent of a plasticizer, such as less than about 10 weight percent dibutyl sebacate and less than about 10 weight percent tnethyl citrate, and a matenal that reduces particle agglomeration dunng the coating process, such as talc. Unless noted otherwise, the weight percents stated in this application are based on the final dry weight of the coated beads.

One skilled in the art also will realize that the BKD vaccine of the present invention can be used in combination with lmmunostimulants, such as 0-glucans. The immunostimulant may be incorporated into the formulations coated onto the microspheres so that the immunostimulant is released by the beads following the administration thereof to fish susceptible to infection by Renibacterium salmoninarum . The beads can be coated so that the immunostimulant is released pnor to the release of the BKD vaccine. This is believed to pnme the immune system. Alternatively, the BKD vaccine of the present application may be released pnor to the immunostimulant. As still another possibility, lmmunostimulants may be administered by a method other than that chosen for the delivery of the BKD vaccine. For instance, the BKD vaccine might be orally administered and the immunostimulant administered by IP injection or by immersion, either pnor to, simultaneously with, or after the administration of the BKD vaccine.

The present invention also provides a method for reducing the infection of fish susceptible to infection by virulent strains of Renibacterium salmoninarum . A preferred embodiment of the method compnses first heating Renibacterium salmoninarum microorganisms to a temperature of at least about 37 °C to produce Renibacterium salmoninarum microorganisms lacking intact cell-surface-associated protein p57. Nonpareil sugar beads, having a preferred mesh size of from about 25 to about 30, then are coated with a first layer compnsing a mixture of a super disintegrant and Renibacterium salmoninarum microorganisms lacking intact cell-surface-associated protein p57. The beads are then coated with a second layer compnsing a pH-sensitive polymenc matenal that is dissolved by an aqueous media having a pH of about 5 Oor greater, thereby producing an enteπc-coated vaccine. The coated beads are orally administered to fish in an amount sufficient to reduce the infection by fish susceptible to infection by Renibacterium salmoninarum . The method may also comprise the step of treating fish susceptible to infection by Renibacterium salmoninarum with an immunostimulant either before, simultaneously with, or after the step of adrmnistenng the vaccine to fish BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a Western blot (A) and total protein stain (B) of Renibacterium salmoninarum cells after treatment at 37 °C followed by formalin incubation at 17 °C.

FIG. 2 is a schematic representation of an oral enteric-coated vaccine according to the present invention.

FIG. 3A-3D are graphs showing the percent survival of fish over time following challenge with Renibacterium salmoninaru , wherein the challenged fish had been IP injected with one embodiment of a vaccine according to the present invention.

FIG. 4 is a graph of titers of Renibacterium salmoninarum antibodies from chinook salmon immunized with p57^~ or p57⁺ whole Renibacterium salmoninarum cells. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

The present invention provides the first known vaccine and method for effectively protecting fish susceptible to infection by Renibacterium salmoninarum microorganisms. A detailed discussion follows concerning how to make the claimed vaccine, as well as how to administer the vaccine to fish. Experimental data also is presented which demonstrates that the vaccine is effective for protecting fish from infection by Renibacterium salmoninarum .

1. DEFINITIONS A number of definitions are provided below. These definitions are provided solely for the convenience of persons reading this disclosure. These definitions are not intended to narrow the scope of such terms to definitions less encompassing than that understood by persons skilled in the art.

1. "Killed vaccines" generally refer to microorganisms which have been heat- treated and thereafter treated with some chemical agent, such as formalin. Renibacterium salmoninarum is heat sensitive. It cunently is believed that heat treatment alone, such as heating to temperatures greater than about 35 °C, is sufficient to kill the bacterium. However, solely for the purpose of caution, the heat-treated bacterium also usually are treated with a chemical agent to produce the killed vaccine.

2. "Protective immunity" is the condition induced by the administration of a vaccine to a fish wherein the susceptibility of the fish to infection by a particular pathogen is reduced.

3. "Susceptible fish" are those species of fish of which Renibacterium salmoninarum is a pathogen and in which the vaccines of the present invention are capable of inducing protective immunity. That is, the microorganism is capable of causing Bacterial Kidney Disease (BKD) in such a fish and the fish is capable of being protected from such disease by vaccination with the vaccines of the present invention. For the purposes of the present invention, "susceptible fish" includes all salmonid fish. Salmonid fish include, but are not limited to, pacific salmon in general (Oncorhynchus sp.), such as rainbow trout (Oncorhynchus mykiss), chinook salmon (Oncorhynchus tshawytscha), coho salmon (Oncorhynchus kisutch) sockeye salmon (Oncorhynchus nerca) and atlantic salmon (Salmo salaή. Both the chinook and coho salmon appear to be particularly susceptible to infection.

4. "Susceptibility to infection" describes the condition of being a host for a particular pathogen and of suffering injury from the disease caused by that pathogen. The condition of "susceptibility to infection" encompasses a range of susceptibilities. The degree of susceptibility of a particular fish to infection by a particular pathogen may be determined by calculating the LD₅₀ value for this pathogen. Fish species less susceptible to infection by a particular pathogen will have a higher LD₅₀ for that pathogen than a more susceptible fish species. 5. "p57" is a short-hand notation which refers to cells of Renibacterium salmoninarum which lack intact cell-surface-associated protein p57.

6. "pST*" is a short-hand notation which refers to cells of Renibacterium salmoninarum which include cell-surface protein p57.

7. "Adjuvant" as used herein refers to any material that enhances the action of a drug or antigen.

8. "Pharmaceutical Excipient" refers to any inert substance that is combined with an active drug or antigen for preparing an agreeable or convenient dosage form.

II. MATERIALS AND METHODS A. Bacterial Strains Bacterial Kidney Disease (BKD) is caused by a fastidious, slow growing bacterium, Renibacterium salmoninaru . The bacteria presents itself as a facultative (i.e., the bacteria is capable of an adaptive response to various environments) intracellular parasite, which also has the ability to survive and multiply within the phagocytic cell. Renibacterium salmoninarum is a gram-positive, short rod (.08-1. OX 0.3-0. j m) bacterium. The bacterium is nonmotile, asporogenous, non-acid fast and encapsulated. The guanine-plus-cytosine (G + C) content of the bacteria averages about 53-mole percent.

It is likely that all isolates of Renibacterium salmoninarum can be used to make vaccines according to the present invention. All strains of Renibacterium salmoninarum produce cell-surface protein p57. And, all strains of Renibacterium salmoninarum are significantly genetically homogenous, more so than other bacterium, regardless of where the bacteria are isolated. The genetic homogeneity of the Renibacterium salmoninarum is a trait fairly unique to the organism. Persons skilled in the art have tried to develop antibodies useful for distinguishing between strains of the bacterium. So far, these efforts have proved fruitless. Thus, "strains" when used in connection with Renibacterium salmoninarum simply refers to the location where the bacterium were isolated, and not to some inherent physiological difference between the isolated microorganisms.

Solely to provide specific guidance as to Renibacterium salmoninarum isolates that have been used to develop vaccines, a first such isolate was cultured from chinook salmon (Oncorhynchus tsawytscha, Oregon) and has ATCC strain number 33209. A second isolate, isolate D6, was isolated from coho salmon (Oncorhynchus kisutch, held in salt water in Oregon). The D6 isolate was obtained from C. Banner of Oregon State University. The D6 isolate also is discussed in Wiens et al.'s Monoclonal Antibody Analysis of Common Surface Protein(s) of Renibacterium salmoninarum . Fish Pathology, 24: 1-7 (1989), which is incorporated herein by reference. All strains used to produce vaccines were stored at -70 ° C prior to culture.

The following Example 1 describes a method for culturing Renibacterium salmoninarum . This and all subsequent examples should, in no way, be construed to limit the scope of the present invention to the particular embodiments described.

EXAMPLE 1 Renibacterium salmoninarum ATCC 33209, or Isolate D6, was grown in one-liter volume portions using a 2.5 liter low form, VWR culture flask. The Renibacterium salmoninarum cultures were grown with intermittent shaking at 17 °C using a KDM-II growth medium prepared according to the method of Evelyn, except without serum supplementation. This medium is discussed in Evelyn et al.'s An Improved Growth Medium for the Kidnev Disease Bacterium and Some Notes on Using the Medium . Bull. Int. Epiz. 78:511-513(1977), which is incorporated herein by reference. The bacteria were grown until an optical density of from about 0.4 to about 0.8 was generated at about 525 nm. This required approximately 7 to 8 days. Seven one-liter volumes of bacteria from the 2.5-liter low-form VWR culture flasks were combined, and then pelleted by centrifugation at 6,000 X g for about 30 minutes. The pelleted cells were then resuspended in 100 ml of cold, phosphate-buffered saline solution (PBS; 0.85% NaCl, lOmM NaP0₄, pH 7.2). The cells were then centrifuged a second time at 6.000X g. Thereafter, the cells were placed in microfuge tubes and frozen at -70°C for storage.

B. Bacterial Extracellular Preparations as Vaccination Control The vaccine preparations of the present invention, which are described in detail below, were evaluated relative to a number of control formulations, including extracellular preparations from Renibacterium salmoninarum . An extracellular protein (ECP) preparation for use as a control was prepared according to the method of Daly et al.'s Agglutination of Salmonid Spermatozoa bv Renibacterium salmoninarum . J. Aquatic Animal Health, 1: 163-164 (1989), which is incorporated herein by reference. The following Example 2 describes a method for obtaining the ECP. EXAMPLE 2

2 to 4 grams of wet bacterial cells were washed with 100 ml of sterile phosphate buffer saline and then pelleted by centrifugation at 6,000 X g for 30 minutes. The centrifuged cells were then resuspended in 100 ml of distilled and deionized water and placed on ice for about one hour. The cells were then repelleted by centrifugation at 6,000 X g. The supernatant was removed and cell-surface proteins were precipitated with the addition of powdered ammonium sulfate. The resulting ECP extract was dialyzed three times against phosphate buffer saline and filter stenhzed by passing the extract through a .45 μm filter. The protein concentration was then determined by the method of Lowry et al.

C. Preparation of Killed Vaccine Lacking Intact Cell-Surface-Associated Protein pS7 A major component of the ECP is a protein having a molecular weight of about 57 kDa. This protein also is known to be a major cell-surface protein. Without limiting the present invention to one theory of operation, it currently is believed that the present vaccine protects fish where previous attempted vaccines and vaccination methods have failed because cell surface protein p57 is removed from the bactenal cells before being administered to fish as a vaccine

Heat treatment is a currently prefened method for removing the cell-surface protein p57. However, the present invention encompasses any methods now known or hereafter developed for removing p57. Although the temperature used to remove the protein may vary, a temperature of about 37 °C is believed to be the optimal temperature for activatmg an autologous senne protease, and therefore for cleaving off the cell-surface protein. See Rockey et al.. Charactenzation of a Renibacterium salmoninarum Senne Protease Which Digests a Mai or Autologous Extracellular and Cell-Associated Protein ■ Can. J. Micro., 37:758-

763 (1991), which is incorporated herein by reference

The vaccine of the present invention is administered as a killed vaccine. The present invention encompasses any methods now known or hereafter developed for killing Renibacterium salmoninarum cells for use as a vaccine. However, it has been determined that heating the cells to a temperature of about 37 °C kills the microorganism. Renibacterium salmoninarum is a relatively heat-sensitive organism In fact, it is likely that temperatures of less than about 37 °C can be used to kill the bactenum However, as the temperature is lowered, the time required to kill the organisms increases If heat treatment alone is used to kill the vaccine, then the temperature likely should not be increased to be much above 55 °C. Otherwise, the senne protease activity may be hindered. As a result, the removal of the p57 cell-surface-associated protein would be affected.

In order to insure that the bacteria were killed pnor to adπunistenng the vaccine denved therefrom to fish, the heat-treated cells first also were fixed with formalin. The step of formalin fixing likely is a superfluous step, and it currently is believed that heating the cells is a sufficient method for killing the bacterial cells

The following Example 3 describes a method for preparing killed p57^" bactenal cells EXAMPLE 3 Frozen harvested cells were prepared as described above in Example 1. The frozen cells were thawed from -70° C and microfuged at 6,000 X g. The centrifuged cells were then weighed and resuspended in a sufficient amount of cold, sterile phosphate buffer to obtain a concentration of about 200 mg cells/ml. The cells were then heated to a temperature of about 37 °C, and this temperature was maintained for about 48 hours. After the heating step had been completed, the cells were again microfuged at 6,000 X g, and then resuspended in a 3 % formalin-phosphate buffered saline solution. The formalin-cell ixture was then cooled to a temperature of about 17 °C, which was maintained for about 10 hours. The cells then were repeatedly washed with phosphate buffered saline solutions and reweighed.

FIG. 1 shows a Western blot (A) and total protein stain (B) of Renibacterium salmoninarum cells following treatment at 37 °C and formalin incubation at 17 °C as described above in Example 3. The Western blot was probed with monoclonal antibody 4D3, which recognizes p57. The lanes represented on the gels are as follows: Molecular weight; Lane 1 shows untreated Renibacterium salmoninarum cells; lanes 2-4 illustrate three separate treatments of Renibacterium salmoninaru . Lanes 2-4 on both the Western blot and the total protein stain clearly indicate the absence of a band corresponding to p57. This demonstrates that the method described in Example 3 effectively removes p57 to below detectable limits, thereby producing p57^" Renibacterium salmoninarum cells. D. Antigen Preparation for Vaccine Comparisons

In order to determine the effectiveness of the killed Renibacterium salmoninarum cells for developing an intraperitoneal vaccine, bacterial antigens were emulsified in Freund's incomplete adjuvant (FIA) using a Virtis 23 mixer set at 100 units for four minutes. The putative antigens used for the intraperitoneal vaccination consisted of approximately 500 μg of heat-treated Renibacterium salmoninarum cells produced as described above in Example 3, 50 μg of cell surface extract obtained as described above in Example 2, and 50 μg from extracellular protein extracted from culture supernatants.

E. IP Vaccination and Challenge Coho salmon were injected with the putative antigen preparations in a total volume of about 0.1 ml. The fish were injected IP and intramuscularly, with a total of about 0.05 ml at each location, using a 22 gauge needle. Booster injections were then given to the fish 45 days after the primary injection. The booster injection consisted of about one half of the volume of the antigen or control preparations used in the primary injection, although the total volume of material injected was the same in both the primary and booster injections. The fish then received a secondary boost 10 days after the first booster shot was administered.

The vaccinated fish were challenged by IP injection. The progress of BKD in fish challenged by natural methods is slow. The onset of the disease may take as long as a year or more, which makes laboratory testing impractical. As a result, the fish were initially challenged by IP injection to accelerate the onset of the disease. The Renibacterium salmoninarum used for the challenge were grown for 7 days in KDM-II and thereafter washed with PBS. The cells were then suspended in sterile PBS to obtain a final optical density of about 0.2 at 525 nm. The fish were then IP challenged with a challenge dose of about 4.1 X 10° cfu/ml. The results of this challenge experiment are summarized in FIG. 3.

FIG. 3 shows the percent survival of coho salmon immunized with various potential immunogenic materials obtained from Renibacterium salmoninarum . Three tanks of salmon (40 salmon/tank) were injected with the various materials. The fish were injected with either saline emulsified in Freund's incomplete adjuvant (see graph 3A), extracellular protein in Freund's incomplete adjuvant (see graph 3B), virtually pure p57 obtained from cell wash and emulsified in Freunds incomplete adjuvant (CW, see graph 3C), and p57^" Renibacterium salmoninarum cells in Freund's incomplete adjuvant. Graphs 3A-3D are illustrated having enor bars which represent two standard errors about the mean for the three trials.

The results summarized by FIG. 3 indicate that fish receiving p57^" had a significantly increased mean time to death following challenge with live Renibacterium salmoninaru . FIG. 3D appears to show that all fish IP treated with p57^" cells die; however, one reason for this is because the fish were challenged with a relatively large concentration of pathogen that would be expected to cause death, even if the fish were successfully immunized by the vaccine. In other words, the large pathogen concentration used for the challenge was intended to cause death; otherwise, the time required for the challenged fish to die would be too long for practical laboratory investigation. However, it is clear from FIG. 3D that the fish treated by IP immunization had a significantly enhanced mean time to death following pathogen challenge.

FIG. 4 depicts the units of activity/ μl for titers of mύ-Renibacterium salmoninarum antibodies produced upon immunization of chinook salmon. Two groups of chinook salmon (60 fish per group) were IP immunized using either p57⁺ or p57^" cells. After 86 days following injection, serum samples were taken and antibody titers were then determined using the procedure of Kaattari et al. The fish then received a booster shot on day 100 post initial inoculation. On day 128, serum samples were again taken and the data is presented in FIG. 4. This data clearly shows that salmon demonstrate a 20-fold higher titer to p57^" cells (C) than do sera taken from fish injected with p57⁺ cells (A'). More specifically, fish receiving p57^" cells had an average titer of about 25,703 units of anti- Renibacterium salmoninarum activity/ μl. Fish receiving p57⁺ cells had an average titer following receipt of the booster shot of about 1,276 units of anti- Renibacterium salmoninarum activity/ μl. Thus, removing p57 cell-surface protein from the bacterial cells used as immunogenic material for treating salmonids significantly increased the immunogenicity of the Renibacterium salmoninarum . Moreover, although oral administration of the p57^" bacterial cells is a preferred delivery method, IP injection also effectively elicits an immune response in fish receiving the bacterial cells.

F. Preparation of Oral Vaccine After the results were obtained for IP vaccination and challenge, a decision was made to try and develop an oral vaccine. Although the present invention is not limited to administering the vaccine orally, oral administration is a currently preferred delivery method. One reason for this is that oral administration apparently stimulates the gut associated lymphoid tissue (GALT) to a greater extent than does IP injection.

Microspheres have been used for the oral delivery of the present vaccine. There are several advantages for using microspheres, including:

(1) The microspheres release the antigen which can then be taken up in the GALT, which is an important part of the secretory immune system.

(2) Entrapped antigens are protected from being degraded by the acidic environment of the stomach. (3) Plural antigens can be administered at the same time.

(4) Pharmaceutical excipients, such as super disintegrants, can be applied to the microspheres.

(5) Additional materials, such as adjuvants, may be administered along with the specific antigen of interest. (6) Controlled/sustained-release formulations are possible.

The present invention provides a BKD vaccine that can be administered orally after the antigenic material is applied to microspheres and subsequently enteric-coated to produce an enteric-coated antigenic microsphere (ECAM). A schematic diagram of one embodiment of an ECAM is illustrated in FIG. 2 as ECAM 2. The illustrated embodiment of the ECAM 2 comprises a sugar bead 10 of conventional type, at least a first antigen coating 12, and an enteric protective layer 14.

One skilled in the an will realize that a number of sugar beads potentially are useful for forming ECAMs. However, without limitation, a currently suitable sugar bead is an NF sugar sphere which can be obtained commercially from Ingredient Technology Corporation, of Pennsauken, New Jersey. The illustrated sugar beads 10 of FIG. 2 are dextrose beads.

The size of the bead is an important consideration for selecting an appropriate sugar microsphere. Certain bead sizes have been found to be too big to pass through the pyloric sphincter of the fish, and therefore may not effectively confer immunity to fish treated with such beads and an oral vaccine. Presently, it appears that a microsphere having a mesh size of from about 10 to about 60 mesh, preferably from about 20 to about 35 mesh, and even more preferably from about 25 to about 30 mesh, will perform satisfactorily for forming ECAMs of the present invention. Sugar beads 10 are coated with a first antigen coating 12. As will be apparent from the preceding discussion, the antigens of choice for the present invention are p57^~ killed bacterial cells. Sugar spheres have been coated with this, and other potential immunogenic materials, using a fluidized bed spray coater. The actual coater used for coating such microspheres was obtained from Labline/PRL, of Melrose Park, Illinois. The antigens were applied to the sugar spheres typically as a gelatinized solution, such as about a 4% weight/volume solution.

After the antigen coating 12 has been applied to the sugar bead 10, an enteric- protective layer 14 is then be applied to the sugar bead. As with the selection of the sugar bead, one skilled in the art will realize that a number of enteric-coating agents may be used. The present invention is directed to any vaccine comprising p57^", including any such material that has been enteric-coated with any coating material now known or hereafter developed.

However, solely by way of example, currently suitable enteric-coating materials are non-toxic polymeric materials which resist dissolution at the pH of the stomach, but which are dissolved once the material passes from the stomach to the pyloric caecum and intestines. Preferably, the polymeric material is dispersible in an aqueous system without the use of organic solvents. The Environmental Protection Agency does not recommend using organic solvents for use in spray-coating procedures. Organic solvents also add to the expense of producing the vaccine. Table 1 below provides a non-exhaustive list of enteric-protecting polymeric materials cunently believed to be useful for forming the vaccines of the present invention.

TABLE 1 ENTERIC COATING POLYMERIC MATERIALS (1) Cellulose Acetate p thalate (CAP) (2) Hydroxypropylmethyl Cellulose Phthalate (HPMCP)

(3) Carboxymethylethyl Cellulose (CMEC)

(4) Hydroxypropylmethyl Cellulose Acetate Succinate (HPMC-AS)

(5) Cellulose Acetate Trimellitate (CAT) (6) Polyvinyl Acetate Phthalate (PAP)

EUDAGRIT BRAND POLYMERS

(7) EUDAGRIT L-30-D and 1 100-55

Poly(ethylacrylate, methacrylic acid) , [copolymer having a 1:1 ratio of monomers; dissolves at pH = 5.5]

(8) EUDAGRIT L 12.5 and L 100 Poly(methacrylic acid, methylmethacrylate) tend to dissolve at pH of from about 5. 8-6. 0. (9 ) EUDRAGIT E, RL, RS and NE . Additional information concerning materials useful for forming coatings for the present invention can be obtained by consulting (1) Osterwald's Properties of Film-Formers and Their Use In Aqueous Systems. Pharmaceutical Research, 2: 14-18(1985), and (2) Aqueous Polymeric Coatings for Pharmaceutical Dosage Forms, edited by J.W. McGinity, Marcel Publishing (1989). Each of these references is incorporated herein by reference. A polymeric material that has been used to form vaccines for the present invention is poly(methacrylic acid-ethylacrylate). This material is commercially available from Rδhm Pharma of Weiterstadt, Germany, as EUDRAGIT ™ L-30D. The polymeric material was applied in the same manner as the antigen to form enteric-protected sugar spheres.

Persons skilled in the art also will realize that additional materials can be used in combination with the enteric-coating materials to form the enteric-coated antigen microspheres. For instance, plasticizers often are used to form pharmaceutical preparations. Pages 17 and 68 of Aqueous Polymeric Coatings for Pharmaceutical Dosage Forms , supra, provide a list of plasticizers commonly used for pharmaceutical preparations. The following Table 2 also provides a non-exhaustive list of useful plasticizers. TABLE 2

PLASTICIZERS

(1) Polyethylene glycol 200 (PEG 200; 200 refers to the average molecular weight)

(2) Polyethylene glycol 400 (PEG 400)

(3) Polyethylene glycol 1000 (PEG 1000) (4) Polyethylene glycol 4000 (PEG 4000)

(5) Polyethylene glycol 6000 (PEG 6000)

(6) Propylene glycol

(7) PVPK-90

(8) Glycerin or Glycerol (9) Diethyl Phthalate

(10) Oleic acid

(11) Isopropyl myristate

(12) Liquid paraffin or mineral oil ( 13 ) Triacetin

(14) Glycerol monostearate (15) Dibutyl Sebacate

(16) Triethyl citrate

(17) Tributyl Citrate

(18) Acetylated monoglyceride

(19) Dibutyl phthalate (20) Acetyl tributyl citrate

(21) Castor oil

(22 ) Glycerol tributyrate Disintegrants, including matenals generally considered by those skilled in the art to be super disintegrants, also often are used in combination with the entenc-coating material to facilitate the disintegration of the microsphere and release of the vaccine. Any disintegrant now known or hereafter developed likely will work for forming the vaccines of the present invention. Soley by way of example, sodium starch glycolate (SSG, Explotab^®, Edward Mendell) is a super disintegrant cunently known to be useful for practicing the present invention.

Vaccines produced using spray-coating devices tend to agglomerate while air- entrained. To alleviate the agglomeration, a "free-flowing "matenal may be added to the coating mixture. A number of "free-flowin "matenals potentially are useful for practicing the invention, and the invention should be interpreted as being broad enough to cover any such additives now known or hereafter developed. Solely by way of example, useful matenals for preventing the agglomeration of the vaccines during the spray coating operation may be selected from the group consisting of talc, magnesium stearate, silicone, silicon oxide, and combinations thereof. For the present invention, it has been found that talc efficiently alleviates agglomeration dunng the coating process, is readily available, and hence is a currently prefened matenal for alleviating agglomeration.

Example 4 below descπbes a method for producing an ECAM according to the present invention. EXAMPLE 4

100 grams of 25-30 or 30-35 mesh size NU-PARELL^® PG, NF Sugar Spheres were obtained from Ingredient Technology Corporation of Pennsauken, NJ. These sugar spheres were loaded into a Lab-line/PRL fluid-bed bottom spray coater preheated to a temperature of about 60 °C (microspheres also have been coated at temperatures of from about 37 °C to about 70 °C). The temperature of the sugar spheres was allowed to equilibrate with that of the coating unit. A 22.5 ml aqueous mixture of a vaccine and a suitable binder comprising from about 0.03 weight percent to about 4 weight percent BKD vaccine (p57^" cells, weight percent based on the weight of the sugar beads to be coated in the coating chamber), and gelatin was prepared. A super disintegrant (either 5%, 9% or 12%) sodium starch glycolate (SSG, Explotab^®, Edward Mendell, Patterson, NY)) was added to this mixture. The 12% SSG released antigen the fastest, and hence is a preferred amount of SSG disintegrant useful for the present invention. The sugar spheres were then placed into the preheated coating apparatus, which was equipped with an 0.8 mm bottom spray nozzle. The operating nozzle pressure of the apparatus was about 18 ± psi, and the blower speed was set at 40 to 50% of full capacity. This caused free movement of the beads in the coating machine. The BKD vaccine was constantly delivered to the nozzle by a peristaltic pump (Gilson Medical Electronics, Middleton, WI) at rate of about 2 to 3 ml/minute.

Once the sugar-sphere coating process was completed, the pellets were dried in the coating chamber for about 5 minutes using the same temperature and air flow stated above. The antigen-coated beads were removed from the coating chamber and kept overnight in an oven that was heated to a temperature of 37 °C. This helped remove residual moisture before the enteric-coating was applied.

An enteric-coating mixture was then formed comprising about 30% (w/v) Eudragit L-30D (PMA-EA, Eudragit ™ L-30D, Rόhm Pharma, Weiterstadt, Germany), less than about 10 weight percent dibutyl sebacate (DBS, Sigma Chemical Co.), less than about 10 weight percent triethyl citrate (TEC, Aldrich Chemical Company, Inc.) and talc (Matheson Coleman & Bell, East Rutherford, NJ). This mixture was applied to the antigen-coated sugar bead in the same manner as the antigen was applied, thereby forming an ECAM according to the present invention. About 20% (w/w) Eudragit L-30D was applied to the beads based on the final dry weight of antigen-loaded beads.

The following enteric-coating formulations have been applied to sugar beads to from coated vaccine microspheres.

TABLE 3

ENTERIC-PROTECTED FILM POLYMER FORMULATION FOR 100 G OF 30-35 MESH-SIZE ANTIGEN-COATED VACCINE BEADS

COMPONENTS GRAMS WT. IN DISPERSION

EUDAGRIT L-30D 15g (Solids) 51.3 (15.4% w/w based on antigen loaded beads; 70% w/w based on total polymer

& Plasticizer solids) TEC

(Triethyl Citrate) 3.3 g Solids) 3.3 (3.3 % w/w based on antigen loaded beads; 15 % w/w based on total polymer & Plasticizer solids)

DBS

(Dibutyl sebacate) 3.3 g (Solids) 3.3 (3.3 % w/w based on antigen loaded beads; 15 w/w based on total polymer & plasticizer solids)

Talc 1 - l g 1.1

(5 % w/w based on total polymer

& plasticizer solids)

Water N/A 51.3 Enteric coating increased weight of microspheres to a final weight of about 123. I g. Total enteric-coating solids = 21.3% w/w based on dried antigen loaded beads. Water was added to make the final suspension about 20% w/v.

TABLE 4

ENTERIC-PROTECTED FILM POLYMER FORMULATION FOR 100 G OF 25-30 MESH-SIZE ANTIGEN-COATED VACCINE BEADS

COMPONENTS GRAMS WT. IN DISPERSION

EUDAGRIT L-30D 14g (Solids) 46.7

(14.0 % w/w based on antigen loaded beads; 70% based on total polymer

& Plasticizer solids)

TEC

(Triethyl Citrate) 3.0 (Solids) 3.0

(3.0 % w/w based on antigen loaded beads; 15 % w/w based on total polymer & Plasticizer solids) DBS

(Dibutyl sebacate) 3. Og (Solids) 3.0

(3.3 % w/w based on antigen loaded beads; 15 w/w based on total polymer & plasticizer solids)

Talc l.Og 1.0

(5% w/w based on total polymer & plasticizer solids)

Water N/A 46.7*

Enteric coating increased weight of microspheres to a final weight of about 121 g. Total enteric-coating solids = 21 % w/w based on dried antigen loaded beads. TABLE 5

ENTERIC-PROTECTED FILM POLYMER FORMULATION FOR 100 G OF 20-25 MESH-SIZE ANTIGEN-COATED VACCINE BEADS

COMPONENTS GRAMS WT. IN DISPERSION

EUDAGRIT L-30D 11.9g (Solids) 39.7

(11.9 % w/w based on antigen loaded beads;

70% based on total polymer

& Plasticizer solids)

TEC

(Triethyl Citrate) 2.55 g (Solids) 2.55

(3.0 % w/w based on antigen loaded beads;

15% w/w based on total polymer

& Plasticizer solids)

DBS

(Dibutyl sebacate) 2.55 g (Solids) 2.55

(3.3 % w/w based on antigen loaded beads;

15 w/w based on total polymer & plasticizer solids)

Talc .85 g 0.85

(5 % w/w based on total polymer & plasticizer solids)

Water N/A 39./

Enteric coating increased weight of microspheres to a final weight of about 117.9g. Total enteric-coating solids = 17.9% w/w based on dried antigen loaded beads.

TABLE 6

ENTERIC-PROTECTED FILM POLYMER FORMULATION FOR 100 G OF 14-18 MESH-SIZE ANTIGEN-COATED VACCINE BEADS

COMPONENTS GRAMS WT. IN DISPERSION EUDAGRIT L-30D 7.7 g (Solids) 25.7

(7.7 % w/w based on antigen loaded beads;

70% w/w based on total polymer

& Plasticizer solids)

TEC

(Triethyl Citrate) 1.65 g (Solids) 1.65

(1.65 % w/w based on antigen loaded beads;

15 % w/w based on total polymer &

Plasticizer solids)

DBS

(Dibutyl sebacate) 1.65 g (Solids) 1.65 (1.65 % w/w based on antigen loaded beads;

15 w/w based on total polymer & plasticizer solids)

Talc .55 g 0.55

(5 % w/w based on total polymer & plasticizer solids)

Water N/A 25.7^*

Enteric coating increased weight of microspheres to a final weight of about 111.6 g. Total enteric-coating solids = 11.6% w/w based on dried antigen loaded beads.

Table 7 lists typical parameters that were used to coat the microspheres using the

Labline/PRL spray coater.

TABLE 7

TYPICAL PROCESS CONDITIONS FOR ANTIGEN OR ENTERIC-FILM COATING

Parameter Amount or Setting

Bed load 100 grams urster Insert bottom spray

Pump peristaltic

Column 7" Wurster

Nozzle Size 0.8 mm

Inlet Temperature 40 or 65°C

Atomization Air 15-18 psi

Fluidization air blower 40-50 % of capacity

Flow Rate 2.3 -6.5 ml/min intermittently^*

Spray Time 0.5-1.5 hour Dry Time about 15 minutes Coating Level very

^*Peristaltic pump was manually turned on or off as necessary to control clumping of beads during the coating process. Vaccines also have been made which included adjuvants, such as lmmunostimulants. lmmunostimulants were added to pnme the immune system of the fish to enhance the immune reponse that occurs as a result of admmistenng the vaccine of the present mvention. β-glucans, which function well as adjuvants, are commercially available in molecular weights of from about 150, 000 to about 700,000. It currently is believed that all such 3-glucans are useful for forming vaccines according to the present invention. Thus, the method for forming ECAMs as descnbed above can be modified to include a /3-glucan adjuvant. One embodiment of a method for producing ECAMs utilizing β-glucans involved spray coating the sugar beads with p57^" cells, a thin coat of AQUACOAT brand coating agent, over which about 140 mg of β-glucan per 100 grams of beads was applied. The beads were then spray-coated with the EUDRAGIT L-30D entenc coating.

One embodiment of a 0-glucan-contaιnιng vaccine is summanzed below in Tables 8 and 9. The data provided in Table 8 represents the matenals used in an AQUACOAT layer, and Table 9 provides the matenals used to form the entenc-coating layer. The process for coating the beads first involved coating microspheres with vaccine, sodium starch glycolate, and gelatin to produce 160 grams of vaccine-loaded beads. A layer of an AQUACOAT composition was then applied over the first microsphere-coating layer. A layer of a /3-glucan having a molecular weight of about 415, 000 was then applied over the AQUACOAT layer. A solution of the 0-glucan (lOmg/ml) was sprayed on the microspheres until 224 mg of the β- glucan was applied to the beads Finally, the microspheres were coated with the entenc- coating composition as summanzed in Table 9.

TABLE 8 AQUACOAT COMPOSITION FOR COATING MICROSPHERES COMPONENTS GRAMS WT. IN DISPERSION

AQUACOAT^® 1.6 g (Solids) 5.33

(1.0 % w/w based on antigen loaded beads, 70% based on total polymer

& Plasticizer solids)

TEC

(Tπethyl Citrate) 0.24 g (Solids) 0.24

(.15 % w/w based on antigen loaded beads, ~11.5 % w/w based on total polymer &

Plasticizer solids) DBS

(Dibutyl sebacate) 0.24 g (Solids) 0.24

(.15 % w/w based on antigen loaded beads; 15 w/w based on total polymer & plasticizer solids)

TABLE 9 ENTERIC COATING FOR BEADS FIRST COATED WITH AQUACOAT

COMPONENTS GRAMS WT. IN DISPERSION EUDAGRIT L-30D 24.6 g (Solids) 82.1 (24.6% w/w based on antigen loaded beads; 70% w/w based on total polymer & Plasticizer solids)

TEC

(Triethyl Citrate) 5.3 g (Solids) 5.3 (5.3% w/w based on antigen loaded beads; 15 % w/w based on total polymer & Plasticizer solids)

DBS

(Dibutyl sebacate) 5.3 g (Solids) 5.3 (5.3% w/w based on antigen loaded beads; 15 w/w based on total polymer & plasticizer solids)

Talc 1.75 g 1.75

(5% w/w based on total polymer

& plasticizer solids)

Water 82.1 Entenc coating mcreased weight of microspheres to a final weight of about 198.5 g. Total entenc-coating solids = 21.3 % w/w based on dned antigen loaded beads. Water was added to make the final suspension about 20% w/v. The formulations discussed above also have been modified as follows. The sugar beads first were coated with a mixture compnsing the BKD vaccine, gelatin, and SSG solution. Thereafter, a layer of about a 1 w/w percent ethyl cellulose (AQUACOAT®) was spray coated onto the sugar beads as a sealing coat. A 0-glucan layer was applied over the AQUACOAT. Finally, the entenc-coating layer (EUDAGRIT L-30-D) was applied. These formulations were designed to release the β-glucan first to pnme the immune system. The BKD vaccine is thereafter released.

However, one skilled in the art will realize that this order could be reversed so that the BKD vaccine was released pnor to the immunostimulant. Moreover, it also would be possible to deliver the β-glucan be means other than entenc-coated microspheres. For instance, if the fish are to be treated with an ECAM, then the immunostimulant (such as a β- glucan) or other desired matenal could be delivered by immersion or IP injection. The immersion or IP ln-ection could be done either pnor to, simultaneously with or after the ECAM is administered to susceptible fish.

G. Oral Vaccine Preparation Utilizing ECAMs Fish were challenged with Renibacterium salmoninarum in order to determine the effectiveness of oral vaccines made according to the present invention. The fish selected for challenge by Renibacterium salmoninarum first were fed ECAMs produced accordmg to the procedure descnbed above. The ECAMs were mixed uniformly throughout fish meal. A sufficient amount of distilled water was added to the fish meal to form a mull. The mull was then extruded using a conventional extruder, and the extruded mixture was cut into pellets. Thus, the ECAMS were incorporated directly into the food supply fed to the fish.

H. Administering Antigens to Fish For oral administration, fish received ECAM -incorporated feed on an every- other-day basis for a total of thirty days. Control fish received lntrapentoneal injections The fish were injected with about

500 μg of the vaccine (in a total volume of about 0.1 ml) antenor to the pelvic fin using a 26- gauge needle The fish received a first booster shot after 30 days, and a second booster shot 10 days after receiving the first booster shot.

Following treatment by both ECAM and IP injection, the fish were allowed to rest for 20 days, and they were then challenged with Renibacterium salmoninarum as descnbed below in Example 5. Each of the five treatments was performed in tnplicate with a total of 25 fish/tank. To monitor the humoral responses and pre-challenge soluble antigen titers, five fish per tank were sacnficed Sera and kidney samples were collected pnor to the challenge. EXAMPLE 5

The D-6 isolate strain of Renibacterium salmoninarum was grown as described above, and the contents of 3 one-liter flasks were combined. The fish were then exposed to Renibacterium salmoninarum by bath challenge as described by Elliot and Pascho (1991), Development of a Waterbome Challenge Procedure for Infecting Salmonids with

Renibacterium salmoninaru . Abstract, 14th Annual AFS/FHS Meetings, 32nd Western Fish Disease Conference, Newport, Oregon, which is incorporated herein by reference. More specifically, fish were placed in tanks and the water level was then reduced from a volume of about 125 liters to a volume of about 25 liters. The flow of water to the tanks was then stopped, and supplemental aeration of the tanks was initiated. Thereafter, viable

Renibacterium salmoninarum was added to the tanks in amounts sufficient to give a final Renibacterium salmoninarum concentration of about 4.2 X lCr cfu/ml as determined by plate count. The fish were exposed to the bacteria for about 22 hours in the standing aerated water. Water flow to the tanks was then resumed and the tanks were allowed to fill at a rate of about 2.8 liters/minute. The bacteria was removed from the tanks through normal effluent flow.

I. ELISA-Based Monitoring of Disease Progress The progress of fish infection following challenge with Renibacterium salmoninarum was accomplished using the monoclonal antibody-based ELISA protocol, with modifications, as described by Rockey et al.. Monoclonal Antibody Analysis of the Renibacterium salmoninarum p57 Protein in Spawning Chinook and Coho Salmon. Journal of Aquatic Animal Health. 3, 23-30 (1991), which is incorporated herein by reference. Example 6 describes a method for monitoring the progress of fish infection.

EXAMPLE 6 Five fish from triplicate challenge treatments were sacrificed in order to monitor levels of soluble antigen. Samples were taken from pre-challenged fish. Samples also were taken at 50, 0 and 150 days following challenge of the fish with Renibacterium salmoninarum . Kidney samples were obtained from each fish and were stored in microfuge tubes held on ice. The kidney samples were then mixed with cold 1 % bovine serum albumin in Tween 20 tris buffered saline (1: 1 weight-volume; tris base, EDTA, NaCl and Tween 20). The samples were homogenized by repeatedly passing them through a 1 ml syringe. Supernatants were collected as described by Rockey et al. ELISA's were then performed on all samples according to the protocol of Rockey et al. Incubation times also were as described by Rockey et al. Optical densities were measured at 405 nm using a Titertek Multiscan Plus plate reader that was purchased from Flow Laboratories. A standard p57 curve was run on every plate. The concentration of p57 in each sample was calculated as described by Rockey et al. using optical- density values generated from the standard curve. It was determined that the assay had a baseline detection limit of about 1.65 ng/ml. Fish were considered to be infected with Renibacterium salmoninarum if the detected level of antigen was at least 3 ng/ l or greater. The data obtained from these ELISA evaluations was statistically analyzed to determine if there were any significant differences between controls and fish treated with vaccines according to the present invention. These results are presented below in Tables 10 and 11. There appeared to be considerable variance between p57 levels in the challenged fish; therefore, all data was log transformed. The results shown below indicate that there was no statistically important difference between control studies and vaccinated fish prior to about 90 days. However, at ninety days the mean p57 levels (ng/ml) for the vaccinated fish was about 20 ng/ml, whereas the control had mean protein levels of about 351 ng/ml. At 150 days the statistical analysis clearly demonstrates that the fish treated orally with p57^~ cells had a significant decrease in the levels of p57, thereby demonstrating the efficacy of vaccines produced according to the present invention.

Table 1 1 shows the results of serum antibody titers, expressed in activity/ μl, throughout the testing period. These results demonstrate that the serum activity levels for p57^~ orally treated fish were much lower than for control fish, or for fish treated by other methods. Specifically, p57^" orally treated fish had an activity of about 126 units/ μl, whereas the mean value for the control fish was about 2060 units/ μl.

TABLE 10

Values expressed are the means of p57 detected (ng/ml) for each particular treatment at each sampling date. Standard errors are in parentheses. Asterisk denotes significant difference from control p<0.01 , **=p <0.03versus control.

Treatment number of fish Mean pre-challenge mean 50 days post mean 90 days post mean 150 days post challenge challenge challenge

"Control 15/sample day < 3 ng/ml * 2.4 (.34) 351 (352) 2070 (1600) ^bOral p57- 15 /sample day < 3 ng/ml 1.2 (.2) 20 (18) 1.9 (.419) *

^CNPP p57- 15/sample day < 3 ng/ml 1.9 (.51) 21 (17) 2.9 (.59) ** ^dOral p57+ 15 /sample day < 3 ng/ml 1.3 (.3) 8701 (8600) 8403 (5603) ^cip p57- 15/sample day < 3 ng/ml 2.14 (.94) 12900 (6400) 220 (173) a = control non-antigen coated beads. b = ECAM delivered p57- whole cells. c = Non-pH protected p57- whole cells. d = p57+ whole cells. e = intraperitoneal injected p57- whole cells.

U = below detection limit of assay

TABLE 11

Values expressed are the means of serum antibody units of activity/ μl serum detected for each particular treatment at each sampling date. Standard errors are in parentheses.

Treatment number of fish Mean pre-challenge mean 50 days post mean 90 days post mean 150 days post challenge challenge challenge ^a Control 15/sample day -ND-* -ND- 2060 (226) 2060 (2276) ^bOral p57- 15 /sample day -ND- -ND- -ND- 126 (436)

^CNPP p57- 15 /sample day -ND- -ND- 938 (2241) 1827 (3146) ^dOral p57+ 10/sample day -ND- -ND- 500 (1881) 3423 (5852) ^eip p57- 15 /sample day 5800 (1500) 42400 (52030) 82000 (210000) 14776 (22065) a = control non-antigen coated beads, b = ECAM delivered p57- whole cells. c = Non-pH protected p57- whole cells, d = p57 + whole cells. e = intraperitoneal injected p57- whole cells. fC = below detection limit of assay.

J. Determination of Antibody Activity Antibody activity titers were ascertained by the use of an enzyme-linked immunosorbent assay (ELISA) as previously described by Arkoosh and Kaattari (1990) Ouantitation of Fish Antibody to a Specific Antigen bv an Enzvme Linked Immunosorbent Assay (ELISA) . Techniques in Fish Immunology , pp. 15-24, which is incorporated herein by reference. Each antiserum was titrated on an ELISA plate that was obtained from Costar E.I. A. /R.I. A., Certified Surface Chemistry of Cambridge, Massachusetts. Formalin-fixed Renibacterium salmoninarum was used as a coating agent at a concentration of approximately 150 μg/ml. Each plate contained a titration of an anti- Renibacterium salmoninarum hyperimmune-serum.

Generally, the detection of serum antibodies is considered a measure of immunity. However, the results of the serum antibody titers seem to indicate that serum antibodies are not necessarily an indication of immunity in the vaccinated fish. Fish receiving oral vaccines survived Renibacterium salmoninarum challenge, but typically had lower serum antibody levels than fish receiving an IP injection. Fish receiving IP injections did exhibit an increased mean-time-to death. All treatment groups, other than the ECAM-delivered p57^~ whole cells and the orally administered, non-pH protected p57^" whole cells, had significantly higher occurrences of p57 in the kidneys of fish challenged with Renibacterium salmoninarum . Without limiting the present invention to one theory of operation, it appears that the best vaccination results are obtained by inducing mucosal immunity. As a result, serum antibody levels are of less importance than mucosal antibody levels. Alternatively, it may be that the protective response in the fish is not mediated by antibodies.

The present invention has been described with reference to several preferred embodiments. Other embodiments of the invention will be apparent to those skilled in the art from a consideration of this specification or practice of the invention disclosed herein. It is intended that the specification and examples contained herein be considered as exemplary only, with the true scope and spirit of the invention being indicated by the following claims.

Claims

1. A vaccine for treating fish susceptible to infection by Renibacterium salmoninarum comprising Renibacterium salmoninarum microorganisms lacking intact cell- surface-associated protein p57.

2. The vaccine according to claim 1 and further including a material selected from the group consisting of adjuvants, plasticizers, pharmaceutical excipients, antigens other than the cells lacking intact cell-surface-associated protein p57, diluents, carriers, binders, lubncants, glidants, aesthetic compounds, such as flavonng and coloring agents, and combinations thereof.

3. The vaccine according to claim 1 wherein the Renibacterium salmoninarum microorganisms have the identifying characteristics of a microorganism selected from the group consisting of Renibacterium salmoninarum ATCC strain 33209 and Renibacterium salmoninarum D6 isolate.

4. The vaccine according to claim 1 compnsing an oral vaccine that includes an entenc coating.

5. The vaccine according to claim 4 wherein the entenc coating is impervious to dissolution in the stomach of the fish.

6. The vaccine according to claim 4 wherein the entenc-coating material is selected from the group consisting of polymenc entenc coating materials that dissolve m a liquid having a pH of about 5 or greater.

7. An oral vaccine for treating fish susceptible to infection by Renibacterium salmoninarum comprising: killed Renibacterium salmoninarum microorganisms lacking intact cell-surface- associated protein p57; and an entenc coating that protects the Renibacterium salmoninarum microorganisms from degradation in the stomach of the fish.

8. The vaccine according to claim 4 wherein the entenc-coating matenal is selected from the group consisting of polymeric enteric coating materials that dissolve in a liquid having a pH of about 5 or greater.

9. The vaccine according to claim 7 wherein the microorganism has identifying characteristics of a microorganism selected from the group consisting of Renibacterium salmoninarum ATCC strain 33209 and Renibacterium salmoninarum D6 isolate.

10. An oral vaccine for treating fish susceptible to infection by Renibacterium salmoninarum , comprising: microspheres having a mesh size of from about 10 to about 60 mesh; a first microsphere coating layer comprising killed Renibacterium salmoninarum microorcanisms lacking intact cell-surface-associated protein p57; and at least a second microsphere coating layer comprising an enteric coating layer that is impervious to dissolution in the stomach of the fish.

11. The vaccine according to claim 10 and further including a material selected from the group consisting of adjuvants, plasticizers, pharmaceutical excipients, antigens other than the cells lacking intact cell-surface-associated protein p57, diluents, carriers, binders, lubricants, glidants, aesthetic compounds, such as flavoring and coloring agents, and combinations thereof.

12. The vaccine according to claim 11 wherein the adjuvant is a disintegrant or a super disintegrant.

13. The vaccine according to claim 11 wherein the pharmaceutical excipient is a

/3-glucan.

14. The vaccine according to claim 11 wherein the enteric-coating layer comprises a polymeric organic material that dissolves in a liquid having a pH of about 5 or greater.

15. The vaccine according to claim 14 wherein the enteric-coating layer comprises ρoly(methylacrylic acid-ethyl acrylate).

16. The vaccine according to claim 11 wherein the enteric-coating layer comprises from about 2 weight percent to about 50 weight percent poly(methylacrylic acid-ethyl acrylate), less than about 10 weight percent dibutyl sebacate, less than about 10 weight percent triethyl citrate, and talc.

17. An oral vaccine for treating fish susceptible to infection by Renibacterium salmoninarum , comprising: microspheres having a mesh size of from about 25 mesh to about 30 mesh; a coating layer comprising killed Renibacterium salmoninarum microorganisms lacking intact cell-surface-associated protein p57; an enteric-coating layer comprising a polymeric organic material that is impervious to dissolution in the stomach of the fish; and a material selected from the group consisting of adjuvants, plasticizers, pharmaceutical excipients, antigens other than the cells lacking intact cell-surface-associated protein p57, diluents, carriers, binders, lubricants, glidants, aesthetic compounds, such as flavoring and coloring agents, and combinations thereof.

18. A method for reducing the infection of fish susceptible to infection by virulent strains of Renibaaerium salmoninarum comprising exposing fish to a sufficient amount of Renibaaerium salmoninarum microorganisms lacking intact cell-surface-associated protein p57 so as to reduce the susceptibility of the fish to infection by virulent Renibaaerium salmoninarum .

19. The method according to claim 18 wherein the step of exposing comprises orally administering a sufficient amount of the Renibacterium salmoninarum microorganisms to the fish so as to reduce the susceptibility of the fish to infection by virulent Renibaaerium salmoninarum .

20. A method for reducing the infection of fish susceptible to infection by virulent strains of Renibaaerium salmoninarum , comprising the steps of: removing cell-surface protein p57 from the Renibaaerium salmoninarum microorganisms; and administering a sufficient amount of the Renibaaerium salmoninarum microorganisms lacking intact cell-surface-associated protein p57 to fish to reduce the susceptibility of the fish to infection by Renibaaerium salmoninarum .

21. The method according to claim 20 wherein the step of removing the cell- surface protein p57 comprises heating Renibaaerium salmoninarum microorganisms to a temperature sufficient to induce protease activity by an autologous serine protease, thereby producing microorganisms lacking intact cell-surface-associated protein p57;

22. The method according to claim 20 wherein the step of removing cell-surface protein p57 comprises heating the microorganisms to a temperature of about 37 °C.

23. The method according to claim 20 and further comprising the step of forming enteric-coated killed Renibaaerium salmoninarum microorganisms lacking the intact cell-surface-associated protein p57 prior to the administering step.

24. The method according to claim 23 wherein the step of administering comprises orally administering the Renibacterium salmoninarum microorganisms lacking the cell-surface-associated p57 protein to fish.

25. The method according to claim 23 wherein the step of forming an enteric- coated microorganisms comprises: providing sugar microspheres having a sufficiently small mesh size to pass through the pyloric sphincter of the fish; applying a first microsphere coating layer to the microspheres, the first microsphere layer comprising killed Renibaaerium salmoninarum microorganisms lacking cell- surface-associated protein p57; and applying a second microsphere coating layer to the microspheres comprising an enteric-coating material that is impervious to dissolution in the stomach of the fish.

26. The method according to claim 25 wherein the second microsphere-coating layer comprises a mixture that includes about 2 weight percent to about 50 weight percent poly(methylacrylic acid-ethyl acrylate), less than about 10 weight percent dibutyl sebacate, less than about 10 weight percent triethyl citrate, and talc.

27. The method according to claim 25 and further including the step of applying to the microspheres a material selected from the group consisting of adjuvants, plasticizers, pharmaceutical excipients, antigens other than the cells lacking intact cell-surface-associated protein p57, diluents, carriers, binders, lubricants, glidants, aesthetic compounds, such as flavoring and coloring agents, and combinations thereof.

28. A method for reducing the infection of fish susceptible to infection by Renibaaerium salmoninarum , comprising the steps of: heating Renibaaerium salmoninarum microorganisms to a temperature of about

37 °C for a sufficient time to produce Renibaaerium salmoninarum microorganisms lacking intact cell-surface-associated protein p57; treating the Renibaaerium salmoninarum microorganisms lacking intact cell- surface-associated protein p57 with formalin; providing microspheres having a mesh size of from about 10 mesh to about 60 mesh; applying a first coating to the microspheres comprising Renibaaerium salmoninarum microorganisms lacking intact cell-surface-associated protein p57; applying a second coating to the microspheres comprising a polymeric material that is impervious to dissolution and/or degradation in the stomach of the fish, thereby producing coated microspheres; applying to the microspheres a material selected from the group consisting of adjuvants, plasticizers, pharmaceutical excipients, antigens other than the cells lacking intact cell-surface-associated protein p57, diluents, carriers, binders, lubricants, glidants, aesthetic compounds, such as flavoring and coloring agents, and combinations thereof; forming a mixture comprising fish food and the coated microspheres; and orally administering a sufficient amount of the mixture to the fish to reduce die infection of such fish by Renibaaerium salmoninarum .

29. The method according to claim 26 wherein the step of coating the microspheres with a second layer first comprises forming a second-layer coating mixture that includes about 2 to about 50 weight percent poly(methylacrylic acid-ethyl acrylate), les than about 10 weight percent dibutyl sebacate, less than about 10 weight percent triethyl citrate, and talc.

30. A method for reducing the infection of fish susceptible to infection by Renibaaerium salmoninaru , comprising the steps of: heating Renibaaerium salmoninarum microorganisms selected from the group consisting of Renibacterium salmoninarum ATCC strain 33209 and Renibaaerium salmoninarum D6 isolate to a temperature of about 37 °C, thereby producing Renibaaerium salmoninarum microorganisms lacking intact cell-surface-associated protein p57; treating the Renibaaerium salmoninarum microorganisms lacking intact cell- surface-associated protein p57 with formalin; providing sugar microspheres having a mesh size of from about 25 mesh to about 30 mesh; applying a first coating to the microspheres comprising a Renibaaerium salmoninarum microorganisms lacking intact cell-surface-associated protein p57; coating die sugar microspheres with a second layer comprising a mixture of from about 2 to about 50 weight percent poly(methylacrylic acid-ethyl acrylate), less than about 10 weight percent dibutyl sebacate, less than about 10 weight percent triethyl citrate, and talc, thereby producing coated microspheres; extruding the coated microspheres through an extruder and cutting the extruded coated microspheres into pellets; forming a mull comprising fish food and the coated microspheres; and orally administering a sufficient amount of the mull to the fish to reduce the infection by such fish by Renibaaerium salmoninarum .

31. A fish vaccine, comprising: an antigenic material; and an enteric-coating material that substantially covers the antigenic material.