WO1996009404A1 - Method for preparing nucleic acids for analysis and kits useful therefor - Google Patents
Method for preparing nucleic acids for analysis and kits useful therefor Download PDFInfo
- Publication number
- WO1996009404A1 WO1996009404A1 PCT/US1995/011566 US9511566W WO9609404A1 WO 1996009404 A1 WO1996009404 A1 WO 1996009404A1 US 9511566 W US9511566 W US 9511566W WO 9609404 A1 WO9609404 A1 WO 9609404A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- sample
- nucleic acid
- lysis buffer
- integer
- moiety
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 70
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 70
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 70
- 238000000034 method Methods 0.000 title claims abstract description 45
- 238000004458 analytical method Methods 0.000 title description 4
- 239000003599 detergent Substances 0.000 claims abstract description 30
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims abstract description 19
- 239000002253 acid Substances 0.000 claims abstract description 14
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims description 59
- 230000003321 amplification Effects 0.000 claims description 30
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 30
- 210000004027 cell Anatomy 0.000 claims description 21
- 239000012139 lysis buffer Substances 0.000 claims description 21
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 20
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 20
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 20
- 239000003153 chemical reaction reagent Substances 0.000 claims description 16
- 108090000623 proteins and genes Proteins 0.000 claims description 10
- 102000004169 proteins and genes Human genes 0.000 claims description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 9
- 239000008004 cell lysis buffer Substances 0.000 claims description 9
- 210000003743 erythrocyte Anatomy 0.000 claims description 9
- 210000000265 leukocyte Anatomy 0.000 claims description 9
- 210000004369 blood Anatomy 0.000 claims description 7
- 239000008280 blood Substances 0.000 claims description 7
- -1 poly(alkylene carbonic acid Chemical compound 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 238000001556 precipitation Methods 0.000 claims description 6
- 125000003545 alkoxy group Chemical group 0.000 claims description 5
- 125000002947 alkylene group Chemical group 0.000 claims description 5
- 125000004432 carbon atom Chemical group C* 0.000 claims description 5
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 claims description 5
- 238000004132 cross linking Methods 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 229910052739 hydrogen Inorganic materials 0.000 claims description 5
- 239000001257 hydrogen Substances 0.000 claims description 5
- 125000004435 hydrogen atom Chemical group [H]* 0.000 claims description 5
- 125000005647 linker group Chemical group 0.000 claims description 5
- 125000003099 maleoyl group Chemical group C(\C=C/C(=O)*)(=O)* 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 4
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 4
- 241000589500 Thermus aquaticus Species 0.000 claims description 4
- 230000002401 inhibitory effect Effects 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims description 3
- 239000000523 sample Substances 0.000 description 21
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 14
- 239000013504 Triton X-100 Substances 0.000 description 11
- 229920004890 Triton X-100 Polymers 0.000 description 11
- 239000003795 chemical substances by application Substances 0.000 description 9
- 239000000463 material Substances 0.000 description 9
- 239000007983 Tris buffer Substances 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 8
- 150000007513 acids Chemical class 0.000 description 7
- 230000009089 cytolysis Effects 0.000 description 7
- 239000011780 sodium chloride Substances 0.000 description 7
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 6
- 238000003556 assay Methods 0.000 description 6
- 239000000872 buffer Substances 0.000 description 6
- 238000003752 polymerase chain reaction Methods 0.000 description 6
- 150000003839 salts Chemical class 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- 239000008188 pellet Substances 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 108010006785 Taq Polymerase Proteins 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 4
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 239000003112 inhibitor Substances 0.000 description 3
- 230000002934 lysing effect Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000000379 polymerizing effect Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 108091005904 Hemoglobin subunit beta Proteins 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 102000000447 Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase Human genes 0.000 description 2
- 108010055817 Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase Proteins 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 238000000137 annealing Methods 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 210000005260 human cell Anatomy 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 238000007826 nucleic acid assay Methods 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- IXQGCWUGDFDQMF-UHFFFAOYSA-N o-Hydroxyethylbenzene Natural products CCC1=CC=CC=C1O IXQGCWUGDFDQMF-UHFFFAOYSA-N 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- IEQAICDLOKRSRL-UHFFFAOYSA-N 2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-(2-dodecoxyethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethanol Chemical compound CCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO IEQAICDLOKRSRL-UHFFFAOYSA-N 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 244000304337 Cuminum cyminum Species 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- 102100021519 Hemoglobin subunit beta Human genes 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 239000005639 Lauric acid Substances 0.000 description 1
- 108010090665 Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase Proteins 0.000 description 1
- 238000010222 PCR analysis Methods 0.000 description 1
- 239000012807 PCR reagent Substances 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 108010019653 Pwo polymerase Proteins 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- ZMZDMBWJUHKJPS-UHFFFAOYSA-M Thiocyanate anion Chemical compound [S-]C#N ZMZDMBWJUHKJPS-UHFFFAOYSA-M 0.000 description 1
- 102000004338 Transferrin Human genes 0.000 description 1
- 108090000901 Transferrin Proteins 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 238000003916 acid precipitation Methods 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 239000012736 aqueous medium Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 230000003196 chaotropic effect Effects 0.000 description 1
- 239000013065 commercial product Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- OJSUWTDDXLCUFR-YVKIRAPASA-N deoxy-bigchap Chemical compound C([C@@H]1CC2)[C@@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@@H]([C@@H](CCC(=O)N(CCCNC(=O)[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO)CCCNC(=O)[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO)C)[C@@]2(C)[C@H](O)C1 OJSUWTDDXLCUFR-YVKIRAPASA-N 0.000 description 1
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 102000018146 globin Human genes 0.000 description 1
- 108060003196 globin Proteins 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 150000003278 haem Chemical class 0.000 description 1
- 231100001261 hazardous Toxicity 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- ZMZDMBWJUHKJPS-UHFFFAOYSA-N hydrogen thiocyanate Natural products SC#N ZMZDMBWJUHKJPS-UHFFFAOYSA-N 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229940070765 laurate Drugs 0.000 description 1
- 238000007834 ligase chain reaction Methods 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- POULHZVOKOAJMA-UHFFFAOYSA-N methyl undecanoic acid Natural products CCCCCCCCCCCC(O)=O POULHZVOKOAJMA-UHFFFAOYSA-N 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000003147 molecular marker Substances 0.000 description 1
- UMWKZHPREXJQGR-UHFFFAOYSA-N n-methyl-n-(2,3,4,5,6-pentahydroxyhexyl)decanamide Chemical compound CCCCCCCCCC(=O)N(C)CC(O)C(O)C(O)C(O)CO UMWKZHPREXJQGR-UHFFFAOYSA-N 0.000 description 1
- OQICDZLCHORSMX-UHFFFAOYSA-N oxirane-2,2,3-triol Chemical compound OC1OC1(O)O OQICDZLCHORSMX-UHFFFAOYSA-N 0.000 description 1
- WVDDGKGOMKODPV-ZQBYOMGUSA-N phenyl(114C)methanol Chemical compound O[14CH2]C1=CC=CC=C1 WVDDGKGOMKODPV-ZQBYOMGUSA-N 0.000 description 1
- ONJQDTZCDSESIW-UHFFFAOYSA-N polidocanol Chemical compound CCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO ONJQDTZCDSESIW-UHFFFAOYSA-N 0.000 description 1
- 229920001983 poloxamer Polymers 0.000 description 1
- 229920001992 poloxamer 407 Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920001451 polypropylene glycol Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 150000004032 porphyrins Chemical group 0.000 description 1
- 230000000063 preceeding effect Effects 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000012521 purified sample Substances 0.000 description 1
- 239000011535 reaction buffer Substances 0.000 description 1
- 230000003381 solubilizing effect Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000013077 target material Substances 0.000 description 1
- 239000012581 transferrin Substances 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 150000008501 α-D-glucopyranosides Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE69535284T DE69535284T2 (en) | 1994-09-21 | 1995-09-13 | Method for the preparation of nucleic acids for analysis and kits therefor |
JP51095996A JP3887014B2 (en) | 1994-09-21 | 1995-09-13 | Method for preparing whole cell-containing sample for nucleic acid amplification, method for amplifying target nucleic acid molecule, kit for recovering nucleic acid from nucleic acid-containing cell for nucleic acid amplification |
EP95934415A EP0784695B1 (en) | 1994-09-21 | 1995-09-13 | Method for preparing nucleic acids for analysis and kits useful therefor |
CA002199835A CA2199835C (en) | 1994-09-21 | 1995-09-13 | Method for preparing nucleic acids for analysis and kits useful therefor |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/309,575 US5538870A (en) | 1994-09-21 | 1994-09-21 | Method for preparing nucleic acids for analysis and kits useful therefor |
US08/309,575 | 1994-09-21 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1996009404A1 true WO1996009404A1 (en) | 1996-03-28 |
Family
ID=23198772
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1995/011566 WO1996009404A1 (en) | 1994-09-21 | 1995-09-13 | Method for preparing nucleic acids for analysis and kits useful therefor |
Country Status (7)
Country | Link |
---|---|
US (1) | US5538870A (en) |
EP (1) | EP0784695B1 (en) |
JP (1) | JP3887014B2 (en) |
CA (1) | CA2199835C (en) |
DE (1) | DE69535284T2 (en) |
ES (1) | ES2274520T3 (en) |
WO (1) | WO1996009404A1 (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001042456A2 (en) * | 1999-12-10 | 2001-06-14 | Genespan Corporation | Isolation and purification of nucleic acids |
EP2465934A1 (en) | 2010-12-20 | 2012-06-20 | Steffen Mergemeier | Extraction of nucleic acids |
DE102011054474A1 (en) | 2011-07-20 | 2013-01-24 | Stratec Molecular Gmbh | System for stabilization, storage and storage of a nucleic acid |
DE202014002379U1 (en) | 2014-03-15 | 2014-08-19 | Ruprecht-Karls-Universität Heidelberg | DNA stabilization system |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3062250B2 (en) * | 1994-03-10 | 2000-07-10 | ジェン−プローブ・インコーポレイテッド | Method of inhibiting inhibition of enzyme-mediated reactions by ionic surfactants |
US6218160B1 (en) | 1997-10-31 | 2001-04-17 | Roche Diagnostics Corporation | Site-specific conjugation of glycoproteins |
US7569342B2 (en) | 1997-12-10 | 2009-08-04 | Sierra Molecular Corp. | Removal of molecular assay interferences |
WO2001013086A2 (en) * | 1999-08-13 | 2001-02-22 | Brandeis University | Detection of nucleic acids |
US20050164204A1 (en) * | 2004-01-27 | 2005-07-28 | Reed Thomas D. | Single use lyophilized rnase reagents, and kits and methods for using same |
JP5292722B2 (en) * | 2007-05-09 | 2013-09-18 | 凸版印刷株式会社 | Nucleic acid amplification method using unpurified blood as a sample |
US9416426B2 (en) | 2008-10-27 | 2016-08-16 | Becton, Dickinson And Company | Assay for Chlamydia trachomatis by amplification and detection of Chlamydia trachomatis pmpA gene |
US8492092B2 (en) * | 2008-10-27 | 2013-07-23 | Becton, Dickinson And Company | Assay for Chlamydia trachomatis by amplification and detection of Chlamydia trachomatis PMPA gene |
CN105164259B (en) | 2013-02-25 | 2018-02-27 | 拜奥卡蒂斯股份有限公司 | The separation of nucleic acid |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4889818A (en) * | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
US5130423A (en) * | 1990-07-13 | 1992-07-14 | Microprobe Corporation | Non-corrosive compositions and methods useful for the extraction of nucleic acids |
US5234824A (en) * | 1990-11-13 | 1993-08-10 | Specialty Laboratories, Inc. | Rapid purification of DNA |
US5294681A (en) * | 1992-03-20 | 1994-03-15 | Affinity Technology, Inc. | Water insoluble cross-linked acid compositions |
US5453493A (en) * | 1992-03-20 | 1995-09-26 | Affinity Technology, Inc. | Method of precipitating proteins from aqueous media with a polyhydroxy polycarboxylic acid |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4908318A (en) * | 1987-09-04 | 1990-03-13 | Integrated Genetics, Inc. | Nucleic acid extraction methods |
JPH03503481A (en) * | 1988-02-09 | 1991-08-08 | メモリアル ブラッド センター オヴ ミネアポリス | Nucleic acid isolation |
US5010183A (en) * | 1989-07-07 | 1991-04-23 | Macfarlane Donald E | Process for purifying DNA and RNA using cationic detergents |
US5128247A (en) * | 1989-08-14 | 1992-07-07 | Board Of Regents, The University Of Texas System | Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources |
US5231015A (en) * | 1989-10-18 | 1993-07-27 | Eastman Kodak Company | Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme |
IT1240870B (en) * | 1990-02-14 | 1993-12-17 | Talent | PROCEDURE FOR THE EXTRACTION AND PURIFICATION OF HUMAN GENOMIC DNA |
US5284940A (en) * | 1990-11-14 | 1994-02-08 | Hri Research, Inc. | Preparation for nucleic acid samples |
GB9100551D0 (en) * | 1991-01-10 | 1991-02-20 | Amersham Int Plc | Method and reagent for eliminating analytical interference in enzymatic analysis from substances used for extraction of intracellular metabolites |
AU664050B2 (en) * | 1991-12-18 | 1995-11-02 | Becton Dickinson & Company | Process for lysing mycobacteria |
CA2137690A1 (en) * | 1992-06-12 | 1993-12-23 | Thomas B. Ryder | Preparation of nucleic acid from blood |
-
1994
- 1994-09-21 US US08/309,575 patent/US5538870A/en not_active Expired - Lifetime
-
1995
- 1995-09-13 JP JP51095996A patent/JP3887014B2/en not_active Expired - Fee Related
- 1995-09-13 WO PCT/US1995/011566 patent/WO1996009404A1/en active IP Right Grant
- 1995-09-13 DE DE69535284T patent/DE69535284T2/en not_active Expired - Fee Related
- 1995-09-13 ES ES95934415T patent/ES2274520T3/en not_active Expired - Lifetime
- 1995-09-13 CA CA002199835A patent/CA2199835C/en not_active Expired - Fee Related
- 1995-09-13 EP EP95934415A patent/EP0784695B1/en not_active Expired - Lifetime
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4889818A (en) * | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
US5130423A (en) * | 1990-07-13 | 1992-07-14 | Microprobe Corporation | Non-corrosive compositions and methods useful for the extraction of nucleic acids |
US5234824A (en) * | 1990-11-13 | 1993-08-10 | Specialty Laboratories, Inc. | Rapid purification of DNA |
US5294681A (en) * | 1992-03-20 | 1994-03-15 | Affinity Technology, Inc. | Water insoluble cross-linked acid compositions |
US5453493A (en) * | 1992-03-20 | 1995-09-26 | Affinity Technology, Inc. | Method of precipitating proteins from aqueous media with a polyhydroxy polycarboxylic acid |
Non-Patent Citations (1)
Title |
---|
See also references of EP0784695A4 * |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001042456A2 (en) * | 1999-12-10 | 2001-06-14 | Genespan Corporation | Isolation and purification of nucleic acids |
WO2001042456A3 (en) * | 1999-12-10 | 2002-01-03 | Genespan Corp | Isolation and purification of nucleic acids |
EP2465934A1 (en) | 2010-12-20 | 2012-06-20 | Steffen Mergemeier | Extraction of nucleic acids |
WO2012084909A1 (en) | 2010-12-20 | 2012-06-28 | Steffen Mergemeier | Extraction of nucleic acids |
DE102011054474A1 (en) | 2011-07-20 | 2013-01-24 | Stratec Molecular Gmbh | System for stabilization, storage and storage of a nucleic acid |
DE202014002379U1 (en) | 2014-03-15 | 2014-08-19 | Ruprecht-Karls-Universität Heidelberg | DNA stabilization system |
Also Published As
Publication number | Publication date |
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US5538870A (en) | 1996-07-23 |
EP0784695B1 (en) | 2006-11-02 |
JP3887014B2 (en) | 2007-02-28 |
DE69535284D1 (en) | 2006-12-14 |
ES2274520T3 (en) | 2007-05-16 |
EP0784695A1 (en) | 1997-07-23 |
DE69535284T2 (en) | 2007-04-19 |
CA2199835C (en) | 2005-08-16 |
JPH10506279A (en) | 1998-06-23 |
CA2199835A1 (en) | 1996-03-28 |
EP0784695A4 (en) | 1999-11-17 |
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