WO1995033492A9 - Methods and compositions for modulation of wound healing - Google Patents

Methods and compositions for modulation of wound healing

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Publication number
WO1995033492A9
WO1995033492A9 PCT/US1995/007395 US9507395W WO9533492A9 WO 1995033492 A9 WO1995033492 A9 WO 1995033492A9 US 9507395 W US9507395 W US 9507395W WO 9533492 A9 WO9533492 A9 WO 9533492A9
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WIPO (PCT)
Prior art keywords
antibody
conjugate
tissue
collagen
fibronectin
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Application number
PCT/US1995/007395
Other languages
French (fr)
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WO1995033492A1 (en
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Publication date
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Priority to AU28234/95A priority Critical patent/AU2823495A/en
Publication of WO1995033492A1 publication Critical patent/WO1995033492A1/en
Publication of WO1995033492A9 publication Critical patent/WO1995033492A9/en

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  • This invention relates to methods and compositions for modulating wound healing.
  • the invention is exemplified by a composition comprising a conjugate of a tissue extracellular matrix-specific binding molecule coupled to an antiproliferative or cytotoxic agent for use in inhibition of wound healing in the eye as an adjunct to glaucoma filtering surgery for use in the treatment of proliferative vitreoretinopathy.
  • Glaucoma encompasses a heterogeneous group of eye diseases characterized by a classical triad of symptoms: elevated intraocular pressure (IOP), optic nerve damage, and progressive visual field loss. These eye disorders affect more than two million Americans and are a leading cause of blindness in the United States.
  • IOP intraocular pressure
  • Glaucomas can be classified according to etiology (primary, secondary or developmental) or to the state of the iridocorneal angle (open or closed).
  • the primary glaucomas are open-angle glaucoma, low-tension glaucoma, and single- closure glaucoma.
  • Secondary glaucomas are the result of a defined local or systemic process which causes elevated IOP, i.e., pigmentary, neovascular, pseudoexfoliative, steroid-induced or lens-induced glaucomas and glaucomas secondary to ocular tumors, trauma, inflammation, hemorrhage and surgery.
  • Developmental glaucomas arise from congenital abnormalities, including developmental anomalies of the anterior chamber angle. The dominant form of glaucoma in the United States and Europe i primary open-angle glaucoma (POAG).
  • POAG primary open-angle glaucoma
  • Surgical techniques include laser trabeculoplasty and various types of glaucoma filtering surgery.
  • Laser trabeculoplasty involves the controlled applicatio of intense light to the trabecular meshwork in order to shrink its collagenous tissue and thereby open drainage channels for the aqueous humor.
  • laser trabeculoplasty reduces IOP by approximately 25 percent and is often used as an adjunct to medical therapy before resorting to more invasive surgical procedures.
  • the initial decreases in IOP observed following trabeculoplasty are often lost after a few months or years.
  • Glaucoma filtering surgery is performed to create a drainage channel for aqueous humor outflow from the anterior chamber in order to lower IOP.
  • GFS Glaucoma filtering surgery
  • glaucoma filtering surgery has the potential to replace drug therapy permanently, its current high failure rate makes it an unattractive choice. Uncontrolled scarring of the surgical site usually occurs and the scar tissue frequen blocks the newly created drainage channel. For this reason, GFS is used primarily on "end stage" glaucoma patients who no longer respond to drug therapy and therefore have no other options.
  • Most successful glaucoma filtering surgery results the creation of a filtering bleb, which is an elevation of the conjunctiva at the surgi site.
  • agents also have undesirable side effects due to their inherent toxicity and the lack of specific localization to the surgical site. Further, the use of antiproliferative agents such as mitomycin C and 5-FU applied directly to the surgical site, often results in inadequate wound healing, leakage of the aqueous humor, hypotony or very low pressure leading to further complications.
  • PVR proliferative vitreoretinopathy
  • This growth and differentiation of cells with the accompanying formation of an epiretinal membrane can eventually lead to a tractional distortion of the retina and eventually detachment of the retina.
  • PVR may occur as a result of accidental or surgical trauma.
  • PVR is currently treated surgically with a procedure called vitrectomy. The operation consists of inserting the tip of a small cutting instrument into the vitreous and removing the cloudy vitreous and scar tissue resulting from the trauma.
  • hyaluronic acid also called hyaluronan
  • Toole, In Guidebook to the Extracellular Matrix and Adlecien Proteins eds. Kreis and Vale, pp. 64-65. (1993).
  • the present invention provides a method and a composition for modulating wound healing in a tissue of interest in a mammalian host.
  • the composition includes a cell proliferation-modulating moiety joined to a targeting moiety that binds with high affinity to a component of the extracellular matrix of the tissue of interest and/or of the plasma membrane of a cell type that is involved in the wound-healing process.
  • the cell proliferation-modulating agent can be either one that inhibits or one that promotes healing of the wound.
  • the method includes the step of introducing the composition into a host, generally in the area of the tissue of interest.
  • the method and compositions find use, for example, in the prevention or treatment of proliferative vitreoretinopathy and in enhancing the success of glaucoma filtering surgery by use of an antiproliferative agent as the cell proliferation-modulating agent to inhibit growth of cells migrating into a wound site.
  • Figure 1 shows a schematic representation of one embodiment of the invention wherein a wound in a tissue of interest is contacted with a composition which includes a conjugate between a cell proliferation-modulating agent and a targeting agent that binds with high affinity to a component of the ECM of the tissue of interest.
  • the cell-proliferation modulating agent is daunomycin attached to the carrier agent albumin, which is bound to the targeting agent which is a monoclonal antibody specific for ECM components.
  • the binding region of the monoclonal antibody binds to a component of the ECM, thus placing the daunomycin in close proximity to the offending fibroblast.
  • FIG 2 shows a schematic representation of another embodiment of the invention wherein the cell-proliferation modulating agent is a cytotoxic agent attached to the targeting agent, which is an ECM-binding protein.
  • the binding region of the ECM-binding protein binds to a component of the ECM, thus placing the cytotoxic agent in close proximity to the offending fibroblast.
  • Figure 3 shows a schematic representation of another embodiment of the invention wherein the cell-proliferation modulating agent is a cytotoxic agent, a ricin A chain, which is attached to the targeting agent, which is a bispecific antibody with specificity both for ECM components and for fibroblasts.
  • the ECM-specific bindin region of the antibody binds to a component of the ECM, and the fibroblast-specific binding region of the antibody binds to fibroblast so that the cytotoxic agent can be internalized by the fibroblast and kill it.
  • the tissue can be any tissue which is characterized by having an extracellular matrix (ECM) composed of one or more components that can serve as a target for the targeting moiety of the conjugate, for example, hyaluronan and polypeptides such as proteoglycans, fibronectin, collagen, certain glycoproteins and the like, and in which migration of particular cell types, such as fibroblasts, macrophages, platelets, polymorphonuclear cells and the like, in the wound site is part of the wound-healing or wound-closure process.
  • ECM extracellular matrix
  • compositions include a targeting moiety that "targets" a cell proliferation-modulating moiety to one or more components of the ECM and/or cells that migrate into a wound site so that growth of cells migrating into the wound site is either increased or decreased, depending upon the nature of th cell-proliferation modulating moiety.
  • a linker agent can be included in the conjugate so that multiple molecules of the proliferation-modulating component can be associated with a single molecule of the targeting moiety.
  • the targeting moiety of the conjugate is a bispecific agent with combined specificity for cell targets such as fibroblasts and phagocytes and components of the ECM in the tissue of interest.
  • composition and method of the subject invention offer several advantages over currently available treatment modalities for glaucoma.
  • Successful filtering surgery can prevent many of the problems associated with medical therapy for
  • 5-FU requires repeated injections (a typical treatment regimen is 1 to 2 injections per day for 14 days) and has a number of undesirable side-effects, which include epithelial and conjunctival ulceration, conjunctiva] wound leaks, retinal toxicity, increased likelihood of ocular infection and pain during administration.
  • a single application is often sufficient for modulating wound healing following GFS.
  • the targeting moiety of the conjugate binds with high affinity to a component of the ECM, and/or a cell migrating into the wound site, the growth-modulating component of the conjugate remains localized in the wound site, creating a high local concentration of the growth- modulating agent and minimizing diffusion of the growth-modulating agent into tissue abutting the wound site.
  • the subject invention offers advantages which include controlled dosage of the growth-modulating agent and prevention of unwanted effects on essential cells in the tissue of interest: localization of the conjugate to the wound site prevents migration to areas of sensitive cells, especially normal cells, from the wound site and sequesters the growth-modulating agent in the region of invading cells. Another advantage is that it is unnecessary to prepare targeting agents for each tissue of interest, as components of the ECM such as hyaluronan, collagen and fibronectin, are common to many tissues. Specificity is instead achieved by the site of introduction of the conjugate. The resulting effect on the cell type can, as appropriate, include complete elimination of an undesirable cell type from the wound site.
  • the wound-healing modulating composition is a conjugate comprising a cell proliferation-modulating agent and a targeting agent (first member of a specific binding pair) that binds with high affinity to a component of the extracellular matrix (ECM) of a tissue of interest or at the plasma membrane of a cell involved in healing of the wound.
  • the binding site on the ECM or the cell is the second member of the specific binding pair.
  • the dissociation constant KJ is about 10 "10 M to 10 ⁇ M, preferably less than 10"*M, most preferably less than 10 "7 M.
  • th Kj will be on the order of about 10 "8 M to 10 ",0 M, preferably less than 10 "9 M.
  • the affinity of the binding domain for the relevant component of the ECM should be sufficient so as to provid specificity of binding to the ECM components.
  • the targeting agent can be a polyclonal or monoclonal antibody with high affinity for collagen, fibronectin, proteoglycans such as chondroitin sulfate, glycoproteins unique to the extracellular matrix of the tissue of interest, or the like.
  • the antibody may be produced as a result of hybridoma formation and expression by the hybridoma, or the supernatant culture, o produced by ascites, or may be a monoclonal antibody fragment, such as FAB, F(ab') 2 , Fv, a recombinant variable region, or the like.
  • the antibody may be of an mammalian species, including, murine, rabbit, human or the like, or combinations thereof, such as chimeric antibodies, having a human constant region and a mouse o other mammalian source variable region.
  • the important characteristic is that the antibody or antibodies bind with high affinity to one or more components of the extracellular matrix of the tissue of interest.
  • the methods for preparing such antibodies are well established. See, for example, Fazekas de St. Groth et al (1980) J. Immunol. Methods. 35: 1.
  • An anima is hyperimmunized with a suitable immunogen, with or without addition of adjuvant.
  • the source of the immunogen can include specimens such as tissues removed during surgery, biopsy specimens, cultured cell lines and the like.
  • the extracellular matrix of the tissue of interest or wound-healing cells which migrat into the wound site such as scleral fibroblasts, macrophages, platelets, and the like may be used as the immunogen, particularly components that are obtainable from th human tissue of interest or human cells where the intended treatment recipient is human, or from another intended recipient animal, where the intended use is a veterinary use or when a conjugate that is cross-reactive with human and animal tissue is desired.
  • the immunogen can be extracellular matrix obtained from the tissue of interest, for example, human scleral tissue, human conjunctival tissue, human vitreous and human epiretinal membrane.
  • purified components obtainable from the extracellular matrix of the tissue of interest can be used as the immunogen, for example, collagen, fibronectin, proteoglycans, glycoproteins, and the like.
  • the isotype can be used as the immunogen, for example, where the tissue of interest is human sclera, the immunogen can be type I or in human collagen, where the tissue of interest is vitreous, the immunogen can be type ⁇ human collagen.
  • a mammal which can be a mouse or other mammal, is hyperimmunized with immunogen according to methods well known to those skilled in the art.
  • antibody-producing cells such as spleen cells, lymph nodes or lymphocytes from an immunized animal are removed and immortalized using methods well known to those skilled in the art.
  • Hybridomas are prepared and antibodies secreted by the hybridomas are screened to identify the clones that secrete antibodies of the desired specificity.
  • Screening of the hybridoma clones can be by binding to components of the extracellular matrix using an enzyme-linked immunoabsorbant assay (ELISA) or by other screening methods which are known to those skilled in the art, including radioimmunoassay and immunohistochemical staining of frozen sections of the tissue of interest.
  • ELISA enzyme-linked immunoabsorbant assay
  • the antibodies also were screened for species specificity and cell type specificity.
  • bispecific antibodies antibodies which bind to epitopes on two different substrates, for example they bind both to a component of the ECM and to a component in the plasma membrane of a particular cell type.
  • the bispecific antibodies can be prepared in a number of ways, including linking or combining a monoclonal antibody specific for ECM components of the tissue of interest with another monoclonal antibody specific tor a cellular target, such as a fibroblast cell or a retinal pigmented epithelial (RPE) cell.
  • the two antibodies are joined using, for example, protein A or G. Mixing of different antibodies with protein A can lead either to precipitation or to an ill-defined product, however.
  • parent hybridoma cell lines each producing a antibody with a desired specificity are fused as described by Milstein and Cuello, (1984) Nature 5:299-304.
  • This technique results in hybridoma cell lines which secrete antibodies of the types produced by each of the parent hybridoma cell lines. With multiple possibilities for heavy and light chain mixing, a heterogeneous population of antibodies can result. However, in some cases the predominant forms are the parental antibodies and a bispecific form.
  • Fused hybridoma cell lines producing the desired bispecific antibodies can be obtained as described by Fazekas De St. Grogh and Scheidegger (1980) J. Immunol Methods 35: 21-1 and other methods known to those skilled in the art.
  • the hybridoma producing the bispecific antibody can then be grown in large scale culture and purified. Since the parental antibodies typically can have distinctly different isoelectric points, purification is achieved by cation exchange chromatography, for example, on Sepharose fast S (Pharmacia). Fractions from the chromatography can then be analyzed for specificity of binding and purified antibody which binds only to the cells of interest, components of the ECM in the tissue of interest, or both the cell of interest and components of the ECM in the tissue of interest obtained.
  • components of the ECM in the vitreous and the sclera Of particular interest as cell types of interest are scleral fibroblasts, particularly human scleral fibroblasts and RPE cells.
  • the targeting agent can be a protein which binds to extracellular matrix components and includes extracellular matrix binding proteins.
  • proteins that bind to hyaluronic acid (HA) also referred to as hyaluronan
  • HA binding proteins include CD44, hyaluronectin/versican, aggrecan and link protein. These proteins share a homologous region containing the HA binding domain.
  • a novel HA binding sequence unrelated to the above has been described by Hard wick, ((1992) J. Cell Biol. 117: 1343-1350).
  • HA binding domains also have been described for fibronectin and fibrinogen. These peptides can then be linked to drugs directly or by the use of carriers. Equivalent peptides would those peptides which bind HA and that show greater than 50% ssequence homology with known peptides binding HA, i.e., link protein.
  • Peptides may be prepared using art accepted methods for solid phase syntheses. Peptides prepared with a terminal cysetine or having a terminal control group as described by Winger et al , Biocanjugate Chem. (1995) 6:323-326) can be utilized for direct coupling to appropriately derivatized carrier proteins conjugated to daunomycin. Carrier proteins are derivatized with thiol reactive groups such as N-succinimidyl-32-pyridyldithio propinate (SPDP). Alternatively, peptides may be synthesized such that they have a terminal hydrazide group. Such peptides also can be prepared using conventional methods for solid phase synthesis.
  • SPDP N-succinimidyl-32-pyridyldithio propinate
  • the terminal amino group is deblocked and treated with succinic anhydride.
  • Succinic anhydride reacts with the amino group to provide a terminal carboxyl group to the peptide.
  • Tert-butyl carbazate (Aldrich) is reacted with this carboxyl group using carbodiimide.
  • An alternative procedure is to react eqimolar amounts of succinic anhydride with tert-butyl carbazate independently. The product of this reaction is then reacted to the deblocked terminal amino group of the peptide using carbodiimide. Final deblocking and decoupling yields the peptide of interest with a terminal hydrazide group.
  • the terminal hydrazide group can then react with the C13 carbonyl group of doxorubicin or daunomycin forming a hydrazone (Yamamoto, et al, (1972) J. Medicinal Chem. 15:872-875).
  • the choice of an agent which provides for modulation of wound healing will depend upon whether inhibition or promotion of wound healing is the desired outcome of treatment.
  • specific proliferation-modulating agents include daunomycin, mitomycin C, daunorubicin, doxorubicin, 5-FU, cytosine arabinoside, colchicine, cytochalasin B, bleomycin, vincristine, vinblastine, methotrexate or the like.
  • toxic agents which may be derived from microorganism or plant source
  • examples include the toxic subunits of naturally occurring toxins such as ricin, ab diphtheria toxin, saporin, and the like.
  • Illustrative toxic subunits include the A cha of diphtheria toxin, enzymatically active proteolytic fragments from Pseudomonas aeruginosa exotoxin-A, ricin A-chain, abrin A-chain, modeccin A-chain, and protei having similar activity found in various plants such as the plants Gelonium multiflorum, Phytolacca Americana, Crown, Tiglium, Jatropha, Curcas, Momordic Charantia, Reachan, the toxin saporin from Saponaria qfficinalis (Thorpe et al , (1985) J.
  • the proliferation-modulating agent can be a growth- promoting agent such as fibroblast growth factor, epidermal growth factor, transforming growth factor-j8, and platelet-derived growth factor.
  • the proliferation-modulating agent and the targeting agent providing for binding to the extracellular matrix can be linked, usually by a bond which is cleavable, either intra- or extracellularly by reduction, hydrolysis, enzymatically, o by a bond which is acid labile.
  • the type of linkage used depends upon a number o factors, but particularly the nature of the proliferation-modulating agent. For example, where the agent is one which must be internalized by a cell to have an effect, such as a toxin molecule or toxin A chain, it is preferable that the linkage to the targeting moiety can be cleaved.
  • the targeting moiety can be linked to a drug either directly or indirectly by carrier molecules such as serum albumin (particularl human serum albumin), polyaminoacids, dextran, and the like, by methods well known to those skilled in the art.
  • carrier molecules such as serum albumin (particularl human serum albumin), polyaminoacids, dextran, and the like.
  • the use of a carrier molecule permits binding of multiple molecules of the proliferation-modulating agent to the linker molecule, the number at least partially dependant upon the size of the carrier. Generally, 10 to 3 molecules of agent are bound per molecule of carrier molecule, preferably 5-20, fo an antiproliferative compound, and 1 to 2 molecules per molecule of carrier molecu for a toxin molecule.
  • linkage used can also be dictated by the cell type which is the ultimate goal of the proliferation modulating activity thus preferably the linkage is a pH labile or acid labile link between the targeting moiety and the proliferation- modulating moiety in cases where the cells have phagocytotic properties which migrate into the wound site, for example, fibroblasts and macrophages.
  • linkages which are susceptible to hydrolysis by enzymes which are present in the extracellular matrix. Convenient linkages thus include disulfides, imides, hydrazones, amides and the like.
  • the wound-modulating conjugates be associated noncovalently or covalently in an amount sufficient to provide the desired amount of cell with a compound such as hydroypropylemethyl cellulose (HPMC), collagen, destrem, etc. modulation, either cell-proliferation inhibition or enhancement.
  • HPMC hydroypropylemethyl cellulose
  • the conjugate can be incorporated into wafers (or other forms) comprising materials such as HPMC, collagen, dextran, etc.
  • the conjugate would not necessarily be bound to the mixture rather than incorporated with these materials. This is intended to cement and aid the delivery of the conjugate.
  • a wafer could be delivered to a surgical site more efficiently than an injection.
  • the conjugate can be associated nonconvalently with the polymer either by entrapment of the agent within the biologically inert polymer or by adsorption on to the biologically inert polymer. Generally the rate of release of the growth-modulating agent from the will be at the rate of about 25 to 50% of the amount bound per hour. Generally, the growth-modulating agent is combined with the solid support by assembly of the various components in a sterile environment and the assembly is maintained in an aseptic environment until use. Other agents also can be employed in conjunction with the process of this invention. For example, all-trans retinoic acid can be used to enhance the receptor- mediated cytotoxicity of ricin A-chain immunotoxins, and anti-inflammatory agents can be used. Corticosteroids Name Trade Name
  • the effectiveness of the conjugate for its intended use may be determined in variety of ways. For example, where the desired effect is inhibition of cell proliferation, particularly proliferation of cells which migrate into an ocular wound, is preferable to test the conjugate using cells as similar as possible to the cell type i the tissue of interest, since the sensitivity of various cell types to growth-modulatin agents may vary significantly.
  • the intended use of the conjuga is in human ocular tissue, it is preferable that human scleral fibroblast cells obtaine from surgical specimens or fresh eye bank tissues are used; conversely, where the intended use is a veterinary one, it is preferable that the cells be from the intended recipient host species.
  • Conjugate effectiveness can be evaluated in any of several bioassays includin in vitro cell culture of scleral fibroblasts and an in vivo model of the intended condition for treatment, such as glaucoma or PVR.
  • an in vitro assay for determining the ability of the conjugate to inhibit or stimulate cell proliferation in vitro, cell culture plates can be coated with an extracellular matrix component of the type most prevalent in the tissue of interest.
  • type or type m collagen is prevalent in scleral tissue
  • type II collagen is prevalent in vitreous.
  • concentrations of a conjugate having a high affinity for the ECM component used to coat the plates is then added.
  • the plates are incubated to allow binding of the conjugate to the well associated ECM component, then washed to remove any unbound conjugate. The plates are then washed to remove any unboun conjugate.
  • Culture plates are then seeded with a sub-confluent concentration of cells from the tissue of interest, for example, scleral or conjunctival -derived fibroblasts or RPE cells, and incubated for a sufficient time to observe the desired effect. Growth is determined for each plate by any method known to those skilled in the art, such as total cell count used to differentiate between live and dead cells, trypan blue dye exclusion, tritiated thymidine incorporation and the like.
  • An effective concentration is defined as the dose which inhibits fibroblast proliferation by at least 70%, preferably more than 80%, and most preferably by more than 95% when compared to control plates, i.e., those to which targeting moiety alone is added, or as the dose that stimulates cell proliferation by at least 50%, preferably more than 100% percent, and most preferably by more than 200% when compared to control plates over a period of 24-48 hours.
  • the tissue(s) of interest are the sclera and/or the conjunctiva of the eye
  • the intended application of the invention is to enhance the success rate of glaucoma filtering surgery, in which a drainage fistula or channel has been surgically created to increase aqueous humor outflow as a means of lowering IOP.
  • the intended application of the subject invention in glaucoma filtering surgery is to control healing of the surgical wound such that it heals, but in the process does not create excessive scar tissue to block the filtering channel and bleb that have been made. Since many of the cells in the tissue abutting the wound site are amitotic (non-dividing), the antiproliferative agent will have no effect on these cells, thus conferring additional selectivity to the use of the conjugate.
  • an in vivo animal model is used for evaluation of the efficacy of the invention for use as an adjunct in glaucoma filtering surgery.
  • a functioning filter is created in ocular tissue of rabbits according to the methods described by Lee, et al , (1987) Ophthalmol. 94:1523.
  • Pigs, monkeys, dogs, or other animals that provide an art-accepted means for testing glaucoma treatment regimens also can be used as the model animal.
  • Various concentrations of the immunoconjugate are administered to the fistula and bleb areas prior to, during, or following surgery. Eyes are then studied in terms of IOP, outflow facility and percent of eyes with functional filters as determined by ocular examination.
  • Efficacy of the immunoconjugate is determined by a reduction in IOP, increase in outflow facility and increase in mean duration of time that functional filters are observed when compared to vehicle-treated (control) animals.
  • Another use of the invention is in the prevention of proliferative vitreoretinopathy (PVR). In this disease, cells migrate into the posterior chamber o the eye following accidental or surgical trauma.
  • PVR proliferative vitreoretinopathy
  • the clinical presentation will vary depending on whether the condition originated from pre-existing retinal tears (rhegmatogenous retinal detachment) or as a result of other ocular diseases such as diabetes, retinopathy of prematurity or from injury. Retinal detachment has been observed following intracapsular surgery and Nd:YAG laser capsulotomy.
  • Various cell types implicated in the formation of PVR include: retinal pigmented epithelium (RPE), glial cells, fibroblasts, macrophages and blood elements.
  • RPE retinal pigmented epithelium
  • fibroblasts fibroblasts
  • macrophages macrophages
  • the usual presentation of PVR is characteristic of the elements of wound healing.
  • Cell type, presence of various growth and differentiation factors, as well as the generation of various extracellular matrix components (ECM) combine to promote the inappropria formation of contractile membranes.
  • Extracellular matrix components in epiretinal membranes have been analyzed and show collagen and fibronectin to be the major constituents.
  • the preferred anim model for PVR which can be used to test the efficacy of the conjugate in treating PVR in rabbits, involves compression and partial detachment of the vitreous humor from the retinal surface, followed by introduction of fibroblasts into the posterior chamber of small numbers of cultured fibroblasts (Chandler, et al, (1986) Arch. Clin. Exp. Ophthalmol. 224:86-91).
  • the bispecific or a monospecific conjugate can be used.
  • the experimental animal for example, a rabbit, is anesthetized and the pupils of the eyes dilated.
  • Cryopexy is performed and seven days following cryopexy, a gas such as perfluorocarbone is injected intravitreally under indirect ophthalmoscopic observation.
  • the gas is left in the eye for about two days after which it is replaced with a balanced salt solution.
  • Seven to ten days after gas-fluid exchange the eyes are examined for indications of cataract, vitreous hemorrhage, or any retinal detachment. Animals with any sign of these conditions are eliminated from the study.
  • Experimental eyes are injected with cells such as fibroblasts directl above the optic disc.
  • the test conjugate is injected into the posterior chamber of th eye both before, at the time of, or following injection of the fibroblasts.
  • a sufficient amount of the growth modulating agent is introduced into the wound site to achieve the desired effect of enhanced wound healing or inhibition of wound healing.
  • Methods for introducing the growth-modulating agent include injection directly into the wound site of a solution comprising the cell-modulating agent.
  • An effective dose of the conjugates for inhibiting wound healing in glaucoma filtering surgery would generally be in the range of 1-500 ug/0.1 ml vehicle, preferably 1-100 ug/0.1 ml vehicle, and most preferably 1-50 ug/0.1 ml vehicle.
  • an effective dose of the conjugates would generally be in the range of 1-50 ug/0.1 ml vehicle, preferably 1-25 ug/ml vehicle, and most preferably 1-10 ug/0.1 ml vehicle.
  • the vehicle may be any pharmaceutically acceptable solution that is compatible with the conjugates, including phosphate-buffered saline, balanced salt solution and the like.
  • Compatible pharmaceutical stabilizating agents, antiseptic agents and the like may also be used in the conjugate solutions as long as they do not adversely interfere with the activity of the wound-healing-modulating conjugates.
  • the growth modulating agent can be introduced before, during or after surgery, preferably at the time of surgery into the wound site.
  • the wound-healing-modulating conjugate may be introduced during the surgery incorporated in a solid support composed of materials including biodegradable polyanhydrides and other biocompatible polymers such as HPMC, dextran or collagen.
  • the wound-healing-modulating agent also can be applied after the surgical incision in the conjunctiva, but before an incision is made the sclera, to allow the agent to bind to the newly exposed target tissue. Alternatively, it may also be applied 1 to 3 days after surgery by injection at or nea the surgical site.
  • the affect of the cell-modulating agent associated with the ECM o cell proliferation will be realized within 3 to 24 hours after cells come in contact wi the cell-modulating agent, depending upon the concentration of the agent used and/o the mode of application. In some applications, such as scarring of a fistula site in GFS, it may take from 7 to 30 days. Blockage of the drainage fistula is accompanied by a concomita rise in IOP and loss of bleb patency. These clinical signs of the failure of the fistul and/or the filtering bleb are evaluated by pneumotonometry or other methods and by visual inspection and digital examination of the bleb site.
  • the subject compositions can be provided as kits for use in one or more operations.
  • Kits will include the growth-modulating conjugate either as a concentrate, including lyophilized compositions (which may be further diluted prior use or at the concentration of use, where the vials may include one or more dosages Conveniently, single dosages may be provided in sterilized containers. Where the containers include the formulation for direct use, usually there will be no need for further reagents for use with the method.
  • Immunoconjugates a. Preparation of Goat Anti-Collagen Antibodies. A castrated male Spanish goat, 3-5 years of age, was immunized with type I human collagen (Calbiochem) emulsified in Freund's complete adjuvant (Difco). The animal was given 50 ug collagen in adjuvant as 6-8 subcutaneous injections (prescapular, post-scapular and lateral lumbar regions) at 0.1 - 0.2 ml per site with a total injection volume of 1.0 ml. Booster injections were given every two weeks for six weeks. Antibodies to type I collagen were detected by ELISA, d e animals were bled and serum obtained by conventional methods. b. Preparation of Affinitv-Purified Anti-Collagen Antibodies.
  • IgG immunoglobulin fraction
  • Serum 360 ml
  • CDR Cell Debris Remover
  • the diluted and clarified serum was applied to a Fast S (Pharmacia) cation exchange column (100 ml bed volume) equibrated in the same buffer used to dilute the serum.
  • the bound IgG fraction was eluted with 50 Mm MES pH 6.0 containing 200 Mm NaCl and dialyzed overnight against phosphate-buffered saline (PBS) at 4°C.
  • PBS phosphate-buffered saline
  • the purified immunoglobulin fraction was applied to a 20 ml gelatin-Sepharose 4B column (Pharmacia). Bound antibody was eluted with 200 Mm glycine-Hcl buffer pH 2.5. Affinity purified antibody (40 ml) was dialyzed overnight against 50 Mm MES buffer pH 6.0 and passed over a 1.5 ml Fast S column equilibrated in the same buffer. Bound antibody was eluted with 50 Mm MES pH 6.0 containing 200 Mm NaCl. The antibody solution was desalted into PBS using a Econo Pac 10 DG column (Bio-Rad). Protein concentration was determined by absorbance at 280 nm.
  • the pH is maintained at 9.0 with the addition of 0.5N NaOH. After 15 min., the pH is adjusted to 7 and 0.4 ml of 1 Hepes buffer added. After incubation for one hour at room temperature, six mg o ethyl-3-(3-dimethylaminopropyl-carbodiimide (Sigma) and six mg N- hydroxysulfosuccmimide (Pierce) is added to the reaction mixture. Six mg of affin purified antibody (10 mg/ml) in 50 Mm Hepes buffer pH 7.0 is added and the reaction allowed to proceed overnight at room temperature.
  • the immunoconjugate purified by passage over a Econo Pac 10 DG desalting column equilibrated with P
  • the concentration of DM is determined by measuring the absorbance at 476 nm (extinction coefficient of 17.3 at lmg/ml).
  • the IgG concentration is determined using the Bradford Coommassie Blue dye binding assay (Bio-Rad).
  • d In vitro Inhibition of Cell Growth with Anti-Collagen Daunomycin Immunoconjugates.
  • the anti-type I collagen-DM conjugate cytotoxicity can be tested on human rabbit scleral derived fibroblasts.
  • rat tail collagen Collagen Corp.
  • 0.2 ml/well 24 well culture plates are precoated with rat tail collagen (Collagen Corp.) 0.2 ml/well, 200 ug/ml and the collagen allowed dry overnight at room temperature. The following day additional wells are loaded with 1.0 ml PBS containing 0.1% BSA and the plates are incubated for an addition 2 hours at 37°C. Wells are washed twice with PBS to remove unbound protein. Dilutions of immunoconjugate in PBS containing 0.1 % bovine serum albumin (BS are prepared and added to wells. PBS containing 0.1 % BSA was added to collage and BSA-treated wells as controls.
  • BS bovine serum albumin
  • the plates are incubated at 37° for 3 hours to allow for immunoconjugate binding, then washed twice with PBS to remove unbou material.
  • a suspension of human scleral fibroblasts in M199 media (Gibco) containing 10% fetal bovine serum (FBS) is added to each well (1.0 ml/well at 1 X 10 4 cells/ml). Plates are incubated at 37° for 48 hours followed by the addition of 2.0 uCi 3H-thymidine (Amersham) and incubation for 24 hours. Following thymidine incorporation, cell monolayers are rinsed three times with PBS and 1.0 5% ice cold trichloroacetic acid (TCA) added to the wells.
  • TCA ice cold trichloroacetic acid
  • the well After 30 minutes on ic the well are rinsed once with 5% TCA and the precipitate dissolved by the addition of 1.0 ml 0.1 N NaOH containing 2% Na 2 CO 3 and incubation at 37°C for one hou Following neutralization of the samples by the addition of 15 ul 12 N Hcl/well, the amount of radioactivity in each well is determined by adding 0.5 ml of each sample to 9.0 ml scintillation fluid and counting in a Beckman scintillation counter.
  • peritoneal macrophage feeder cells were prepared. Five BALB/c mice were sacrificed by CO-, asphyxiation and their peritoneal cavities flushed with 5 ml sucrose solution (0.34M). Washings were pooled, counted and centrifuged. Cells were resuspended in Iscoves-modified Dulbeccos containing hypoxanthine, aminopterin and thymidine (IMDM) media to a concentration of 6 X 10* cells/ml. The cells were then distributed in ten 96 well plates at 100 ul/well and incubated at 37°C in a CO 2 incubator. The P3-X63/AG8.653 myeloma cell line (ATCC # CRL 1580) was grown in
  • IMDM containing 10% FBS IMDM containing 10% FBS.
  • the cells were maintained in log phase growth at low density and split 1:2 the day before the fusion.
  • On the day of fusion myeloma cells were collected and washed in IMDM media with 10% serum. The final wash and resuspension was in IMDM without serum. Cells were suspended to a concentration of 1 X10 7 cell ml. All procedures were performed at room temperature.
  • the spleen from each immune mouse was removed aseptically and minced to a single cell suspension.
  • Cells were suspended in 10 ml IMDM and large clumps allowed to settle for 10 minutes.
  • Nine ml of cell suspension were transferred to a 50 ml centrifuge tube, made up to 50 ml with IMDM and cells counted with hemocytometer. Cells were then centrifuged at 200 X g for 10 min and suspended 1 X 10 7 cells/ml in IMDM.
  • the preparation of myeloma and spleen cells was carri out simultaneously. For fusion, 10 ml of myeloma and 6 ml of spleen cells were combined with
  • IMDM media containing hypoxanthine, aminopterin and thymidine (HAT) and 20% FBS and plated on the previously prepared macrophage feeder cells to give approximately 1 X 10 4 cell/well Cells were refed on days 5, 12 and 15, with half (100 ⁇ l) of the media removed and replaced.
  • HAT aminopterin and thymidine
  • 6G10 0.01 0.02 1.0 'ELISA assays were performed in 96-well polystyrene test plates. Antibodies were detected using goat anti-mouse IgG coupled to peroxidase (Kirkegaard and Perry). ABTS was used as the substrate. Absorbance readings were determined with a Dynatech plate reader at 410 nm. 2 Rabbit vitreous diluted in PBS and dried overnight at room temperature. 'Bovine plasma fibronectin diluted in PBS and incubated overnight at 4 ⁇ C. 'Human sclera tissue extracted in PBS using a Dounce homogenizer and dried overnight at room temperature.
  • Hybridomas cells were cultivated in cell culture media, harvested by centrifugation and washed PBS. Cells (1-5 X 10 6 ) were injected intraperitoneally into adult females BOBA/C mice. Mice had been primed by injection of 0.2ml 2,6,10,14-te ⁇ tramethyl decanoic acid (Pristane, Sigma) ten to 14 days prior to injection of cells. Ascities fluid was harvested from the mice within three weeks following injection of cells. The Ascities food was centrifuged at 3,000 x g for ten minutes, cooled and stored at -20 C C.
  • Preparation of Murine Monoclonal Antibodies to Ocular Tissues a. Preparation of Antibodies Binding Scleral Fibroblasts and Retinal Pigmented Epithelial Cells. Preparation of hybridomas secreting monoclonal antibodies to scleral fibroblasts and retinal pigmented epithelial cells followed standard procedures developed in a number of laboratories (Fazekas de St. Groth (1980); and Goding (1986). Individual BALB/c mice were given interperitoneally human scleral fibroblasts in PBS. Subsequent injections were given intravenously. Three days prior to fusion, mice were given a final boost of cells by an intravenous injection.
  • 'ELISA assays were performed in 96-well polystyrene test plates. Individual cell suspensions were added to wells in M199 containing 10% FBS and allowed to
  • HSF human scleral fibroblast
  • S RSF rabbit scleral fibroblast
  • RPE pig retinal pigmented epithelial cells
  • TK- thymidine kinase deficient
  • 3D4 and 7H1 cells (secreting antibodies binding cells as described above) were made hypoxanthine guanine phosphoribosyl transferase deficient (HGPRT-) by incubation in culture medium containing 8-azaguanine (3.3 x 10-5 M). Following incubation in respective media surviving cells were subcloned by limiting dilution (0.5 cells/ well) in 96 well culture plates and the subclones tested for viability in medium containing hypoxanthine, aminopterin and thymidine (HAT). Subclones sensitive to HAT and producing antibody were selected for fusion. TK- 7G12 cells were fused with HGRPT- 3D4 and 7H1 cells as described in Example 2.
  • 1B4 hybridoma cells prepared as described in Example 3, were grown in IMEM containing 10% FBS in spinner culture. Three liters of culture supernatant were obtained and clarified by passage over CDR, concentrated to 500 ml by ultrafiltration, diluted 1:10 with 50 Mm MES buffer pH 5.6 and applied to a Fast S cation exchange column. 1B4 antibody was batch eluted with MES buffer containing NaCl. The column was first treated with buffer containing 125 Mm NaCl. Parental 3D4 antibody eluted at this concentration. 1B4 was then eluted with MES buffer containing 175 Mm NaCl. Parental 7G12 antibody was eluted with 250 Mm NaCl in MES buffer. The eluted bispecific antibody was further purified over protein A-Sepharose (Pharmacia) and the eluted antibody desalted over an Econo Pac 10 DG column equilibrated with PBS.
  • 1B4 antibody was batch eluted with
  • Purified 1B4 antibody was treated with a 7 fold molar excess of SPDP (1 mg/ml in ethanol) incubated for 1 hour at room temperature and desalted over a Econo Pac 10 DG column equilibrated with PBS. SPDP derivitized 1B4 was mixed with ricin A and incubated for 2 hours at room temperature followed by 4°C overnight.
  • Cytotoxic activity of 1B4-RA was tested essentially as described in Example Id. Briefly, 24-well culture plates were precoated with rabbit vitreous diluted in PBS (0.2 ml/well, 6.2 ug/ml of protein) and dried overnight at room temperature. The following day wells were fixed by the addition of absolute ethanol for 30 minutes at 4°C and rinsed with M199 containing 10% FBS. Additional wells were loaded with 1.0 ml PBS containing 1.0 % BSA and the plates were incubated for an additional hour at 37°C. Wells were washed twice with M199 containing 10% FBS.
  • Dilutions of 1B4-RA in M199 containing 10% FBS were prepared (250 ul/well at 250 ug/ml) and added to triplicate wells.
  • M199 containing 10% FBS was added to triplicate wells as a control and the plate incubated at room temperature for 2 hours to allow for immunotoxin binding, then washed three times with PBS to remove unbound material.
  • a suspension of ME180 cells in M199 containing 10% FBS was added to each well (2 X 10 4 cells in 1.0 ml/well). Plates were incubated at 37°C for 48 hours followed by the addition of 2 Uci per well 3H-leucine (Amersham) and incubation for 24 hours.
  • IB4-RA 3,272 18,211 'individual culture wells were coated with a solution of rabbit vitreous diluted in PBS and then treated with 1B4-RA. Following incubation to allow for immunotoxin binding, the wells were rinsed with PBS to remove unbound conjugate and ME 180 cells in M199 containing 10% FBS were added. Following incubation, the ability of cells to incorporate 3H-leucine was determined.
  • Example 5 Preparation and Assay of 3A3-BSA-DM Immunoconjugates a. Modification of Daunomycin using Cis-Aconitic Anhydride. Thirty mg of daunomycin (Sigma Chemical Co.. St. Louis MO) was dissolved in 1.8 ml of ice cold water. Bicarbonate buffer (1.2 ml of a 1M solution, pH 9.0) was added and the solution was placed on ice with stirring. A solution of 0.15 ml dioxane containing 15 mg of cis-aconitic anhydride was added dropwise. The pH was maintained at 9.0 by the addition of 0.5 N NaOH. The reaction mixture was incubated for 15 minutes at 4°C with stirring.
  • Bicarbonate buffer 1.2 ml of a 1M solution, pH 9.0
  • Bovine serum albumin (Sigma Chemical Company) was dissolved in 0.1 M MES buffer, pH 6.0. Twenty mg BSA in 1.12 ml was added to 3.2 ml of the daunomycin solution prepared above with stirring. The reaction vial was incubated overnight at room temperature in the dark to give BSA-DM. c. Introduction of Free Sulfhydryl Groups on BSA-DM.
  • BSA-DM was separated from free DM by desalting over a Econo Pac 10 DG column equilibrated with 50 Mm MES buffer pH 6.0. Limited reduction of disulfide bonds in the BSA-DM was performed by adding dithiothretiol (DTT) to a final concentration of 50 Mm followed by incubation at room temperature for 30 minutes. DTT was removed by desalting over a Econo Pac 10 DG column equilibrated with PBS containing 1 Mm EDTA. As a final step the BSA-DM solution was concentrated to a final volume of 1.1 ml by ultrafiltration (molecular weight cutoff of 10,000 daltons) using a Centricon 10 (Amicon). A ratio of 22: 1 DM molecules per BSA molecule was obtained. d. Modification of Antibody 3A3 with SPDP.
  • 3A3-(LC)-SPDP (0.9 ml) was added to 0.250 BSA-DM prepared from Example 5c and incubated for one hour at room temperature followed by overnight incubation at 4°C.
  • One ml of this mixture was diluted to 10 ml with 50 Mm MES pH 6.0 and applied to a Fast S cation exchange column (1.0 ml bed volume) equilibrated in the same buffer. Unconjugated BSA-DM did not bind to the column.
  • the column was rinsed with starting buffer and bound immunoconjugate was eluted with 20 Mm phosphate buffer containing 300 Mm NaCl pH 7.2.
  • the 3A3-BSA-DM immunoconjugate was collected in a 1.2 ml fraction, f.
  • 0.1 % BSA in PBS 107,465 'individual culture wells were coated with solutions of fibronectin and then treated with 3A3-BSA-DM and PBS containing 0.1 % BSA. Following incubation to allow for immunoconjugate binding, the wells were rinsed with PBS to remove unbound conjugate and MRC-5 fibroblasts in M199 containing 10% FBS were added. Following incubation, the ability of cells to incorporated 3H-thymidine was determined. Data represent average of triplicate samples.
  • DM-BSA with free sulfhydryl groups was prepared as described in Example 5.
  • Bovine plasma fibronectin (0.9 ml) (Calbiochem) at 1.0 mg/ml in PBS was treated with a 5-fold molar excess of (LC)-SPDP (1 mg/ml in ethanol) and incubated for 1 hour at room temperature.
  • (LC)-SPDP-fibronectin (0.9 ml) was mixed with 0.25 ml BSA-DM and incubated for one hour at room temperature followed by overnight incubation at 4 C C to produce Fibronectin-BSA-DM conjugate, b. Cvtotoxic Activity of Fibronectin-BSA-DM.
  • Gelatin (Sigma) was diluted to 100 ⁇ g/ml in water and 0.2 ml aliquots were added to wells of a 24 cell culture plate and allowed to dry overnight at room temperature. Following incubation, wells were rinsed twice with PBS to remove unbound material.
  • Each rabbit receives a subconjunctival injection at the operative site in one eye of 25 ug conjugate in 0.1 ml PBS two hours prior to surgery.
  • the other eye receives a similar injection of 0.1 ml PBS as a control.
  • the rabbit is placed under general anesthesia, a lid speculum placed, a limbal-based conjunctival portame is made approximately 8 mm posterior to the li bus and sharp and blunt dissection performed until the cornea scleral limbus is well visualized.
  • a triangular partial thickness scleral flap is then developed based at the limbus to approximately 50% scleral depth, and then an entry wound into the anterior chamber is made with a sharp 15 degree razor knife.
  • a 1 mm x 3 mm sclerostomy is then performed to excise the tissue under the partial thickness flap.
  • a peripheral iridectomy is performed with Vannus scissors and curved jewelers' forceps. The flap is sutured in place with 10-0 nylon suture and the conjunctiva closed with a running absorbable suture.
  • the animals are then given a combination antibiotic/steroid ointment applied to each eye, are kept warm, and observed every hour for eight hours and then every four hours the following day.
  • the rabbits are given subconjunctival injections in both eyes as above.
  • Daily observations continue thereafter with the instillation of the antibiotic steroid ointment daily for approximately 21 days.
  • Examinations including a routine ophthalmic examination for bleb patency, toxicity and complications, slit lamp biomicroscopy and pneumotonometry, are performed daily for the first five days after surgery, then every third day for eight weeks. Observations, including variations in IOP, are subjected to standard statistical analysis to look for maintenance of lowered IOP.
  • Example 8 Effect of 3A3-BSA-DM Conjugate on IOP after Filtering Surgery in Beagles with Glaucoma
  • Beagle dogs are naturally susceptible to glaucoma and are well accepted as a model for human glaucoma (King et al. (1991) Am. J. Vet. Res. 52:2067-2070).
  • Two groups of six beagles each with glaucoma, as characterized by IOP greater than 30 mm Hg in one or both eyes receive glaucoma filtering surgery in one glaucomatous eye; one group receives 3A3-BSA-DM conjugate and the other group is treated with vehicle control.
  • Beagles will be given a preoperative eye examination with a Zeiss slit lamp biomicroscope.
  • Preoperative IOP will be obtained from the average of three measurements by pneunototometry, using an Applanation Pneumatograph (BioRad), after the installation of one drop of 0.5% proparacaine HC1 to each eye.
  • BioRad Applanation Pneumatograph
  • Each dog receives a subconjunctival injection at the operative site in one eye of 25 ug conjugate in 0.1 ml PBS two hours prior to surgery.
  • the other eye receives a similar injection of 0.1 ml PBS as a control.
  • the dog is placed under general anesthesia, a lid speculum placed, a limbal-based conjunctival portame is made approximately 8 mm posterior to the limbus and sharp and blunt dissection performed until the cornea scleral limbus is well visualized.
  • a triangular partial thickness scleral flap is then developed based at the limbus to approximately 50% scleral depth, and then an entry wound into the anterior chamber is made with a sharp 15 degree razor knife.
  • a 1 mm x 3 mm sclerostomy is then performed to excise the tissue under the partial thickness flap.
  • a peripheral iridectomy is performed with Vannus scissors and curved jewelers' forceps. The flap is sutured in place with 10-0 nylon suture and the conjunctiva closed with a running absorbable suture.
  • the animals are then given a combination antibiotic/steroid ointment applied to each eye, are kept warm, and observed every hour for eight hours and then every four hours the following day.
  • the beagles are given subconjunctival injections in both eyes as above.
  • Daily observations continue thereafter with the instillation of the antibiotic steroid ointment daily for approximately 21 days.
  • Examinations including a routine ophthalmic examination for bleb patency, toxicity and complications, slit lamp biomicroscopy and pneumotonometry, are performed daily for the first five days after surgery, then every third day for eight weeks. Observations, including variations in IOP, are subjected to standard statistical analysis to look for maintenance of lowered IOP.
  • Vitrectomy in Rabbits The refined experimental PVR model involving vitreous gas compression and gas-fluid exchange as described by Chandler et al , (1986) is used to test in vivo activity efficacy of 1B4-RA immunotoxin in rabbits; i.e., to test the ability of the compound to bind in vivo as well as its ability to exert a cytotoxic effect in vivo.
  • Two groups of six Dutch-Belted rabbits each weighing 1.5-2.0 kg are used in the study. All rabbits undergo vitreous gas compression and gas-fluid exchange, and one group receives the immunotoxin, while the other receives a vehicle control.
  • the gas is left in the eye for 2 days after which it is replaced with BSS. Seven to 10 days after gas fluid exchange, eyes are examined for indications of cataract, vitreous hemorrhage, or any detachment. Animals with any sign of these conditions are eliminated from the study. Experimental eyes are injected with 25,000 fibroblasts directly above the optic disc. Cultured rabbit fibroblasts (described above) are harvested from cell culture by trypsinization and resuspended in BSS to achieve a final cell density of 25,000/0.1 ml for intravitreal injection.
  • one group of rabbits receives an intravitreal injection of 25 ug 1B4-RA in 0.1 ml PBS.
  • the other group receives an intravitreal injection of 0.1 ml PBS.
  • Example 10 Conjugation of Daunomycin to Carrier Proteins: Preparation of DM-BSA-DPDPB Forty mg of l-ethyl-3-dimethylaminopropylcarbodiimide (EDC) (Pierce) and 80 mg N-hydroxysulfosuccinimide (sulfo-NHS) (Pierce) were added to 1 ml of bovine serum albumin (BSA) (Sigma) dissolved in 2-(N-Morpholino)ethanesulfonic acid buffer (MES), (10 mg/ml in 50 mM MES pH 6.0) and incubated for one minute.
  • EDC l-ethyl-3-dimethylaminopropylcarbodiimide
  • sulfo-NHS N-hydroxysulfosuccinimide
  • the reduced BSA-DM was desalted over a 1.5 x 32 cm Sephadex G-25 column equilibrated with PBS containing 1 mM EDTA.
  • One 4-di-3'-(2'pyridyldithio)-propionamido) butane (DPDPB) (Pierce) (0.1 ml at 10 mg/ml in DMSO) was added per ml of BSA-DM and allowed to react for 45 minutes at room temperature. Following incubation, unconjugated reactants were removed by desalting over a 1.5 x 32 cm Sephadex G-25 column equilibrated with PBS containing 1 mM EDTA.
  • BSA-DM-DPDPB conjugate was used within one hour for coupling to ECM binding proteins as described below.
  • DM-BSA-DPDPB (17 ml containing 0.13 mg daunomycin/ml) was mixed with 30 ml of reduced 7G12 Fab' (1.4 mg/ml) and incubated for one hour at room temperature followed by 48 hours at 4°C. Following incubation, the Fab '-BSA- DM conjugate was centrifuged to remove insoluble precipitates. The supernatant was passed through a 0.45 micron filter and applied to a anion exchange column (HITrap Q, 2 ml, Pharmacia) equilibrated with PBS. Bound conjugate was eluted with PBS containing 2 M NaCl.
  • Fractions containing 7G12 Fab'-BSA-DM were pooled and applied to a 1.5 x 28 cm Sephadex G-25 column equilibrated with PBS. Fractions containing protein and daunomycin eluting in the column void volume were pooled and stored at -20°C.
  • DM-BSA-DPDPB Collagen Binding Domain of Fibronectin
  • FnCD Collagen Binding Domain of Fibronectin
  • the digested fibronectin was applied to a 27 ml gelatin sepharose (Pharmacia) column and the column was washed with 50 mM Tris buffer pH 8.0 containing 10 mM EDTA. Collagen binding fragments were eluted from the column with 4 M urea in 50 mM Tris buffer, pH 8.0 and fractions containing proteins as determined by absorbance at 280 nm were pooled. Collagen binding fragments containing the fibronectin collagen binding (FnCD) domain were desalted into 50 mM Tris pH 8.0 using a 5 x 15 cm Sephadex G-25 column and pooled. FnCD was then applied to a 2 ml HITrap Q column equilibrated with the same buffer.
  • FnCD fibronectin collagen binding
  • FnCD fractions were eluted with 50 mM Tris pH 8.0 containing 1 M NaCL, pooled and desalted into 0.2 M sodium phosphate buffer pH 8.0 containing 1 mM EDTA using a 1.5 x 28 cm Sephadex G-25 column.
  • Purified FnCD was treated with a fivefold molar excess of 2-imminothiolane-HCl (2-IT) (Pierce) and incubated for 60 minutes at 30 C C. Following incubation, the derivatized FnCD was desalted into PBS using 1.5 x32 cm Sephadex G-25 column equilibrated with PBS containing 1 mM EDTA.
  • FnCD-2IT (15 ml, 4.86 mg/ml) was mixed with DM-BSA-DPDPB (22.4 ml, 0.28 mg/ml) prepared as described above. The mixture was incubated 2 hours at room temperature and 48 hours at 4°C.
  • FnCD-BSA-DM was purified by passage over an anion exchange chromatography (HITrap Q, 5 ml column) equilibrated with PBS. The FnCD- BSA-DM conjugate was eluted from the column using 2 M NaCl in PBS. Fractions containing protein and daunomycin were pooled and desalted over a 1.5 x 28 cm Sephadex G-25 column and stored at -20°C.
  • Hyaluronic Acid Binding Peptides A peptide binding hyaluronic acid (Goetinck, et al, (1987) J. Cell Bio. 105:2403-2408) containing a cysetine at the amino terminal end was synthesized. The peptide sequence is shown below. H-Cys-Ala-Gly-Txp-Leu-Ser-Asp-Gly-Ser-Val-Gn-Tyr-Pro-Ile-Thr-
  • Fmoc amino acids were activated using one equivalent of 0.45M 2-(lH-benzotriazol-l-yl)l,l,3,3-tetraethyl-uronium hexaflurophoshate (HBTU)/ 1 hydroxybenzotriazole (HOBt) solution and two equivalents of N,N- diisopropylethylamine (DIEA). Synthesis was started with 2-chlorotrityl resin. Fmoc protecting groups were removed using 25% piperidine/NMP.
  • TFA trifluroacetic acid
  • a scavenger mixture (0.75 g phenol, 0.25 ml EDT, 0.5 ml thioanisole, 0.5 ml water and 10 ml TFA) for three hours at room temperature.
  • the peptide was purified to greater than 95% purity by reverse phase HPLC using a C18 column with an AB gradient from 0% B where A is 0.1 % TFA in water and B is 0.08% TFA in acetonitrile.
  • a peptide binding hyaluronic acid was synthesized using standard Fmoc solid phase synthesis chemistry starting with Wang resin and using appropriate N-Fmoc-amino acids. Side chain protecting groups were tert-Butyl ethers and esters for Asp, Ser and Glu; Boc for Lys; and trityl group for Asn. Fmoc removal was performed with piperidine-DMF, followed by washing with DMF.
  • Coupling was achieved by combining solid Fmoc-amino acids, HoBt and benzotriazol-1-yl-oly tris (dimethylamino) phosphonium hexafluorophosphate (BOP) and n-methylmorpholine (NMM) under nitrogen at 25 °C. Following the addition of the last amino acid residue and deblocking of the terminal NH2 group, the peptide was treated with an excess of succinic anhydride. Following washing, an excess of tert-butyl carbazate (Aldrich) was added and coupled to the terminal carboxyl group using carbodiimide. Protected peptide resin was then dried in vacuum and cleaved with TFA-scavenger mix solvent. After four hours at 25 °C, the cleavage mixture was filtered, precipitated with ether, collected by centrifugation and lyophilized. The peptide sequence with the terminal hydrazide group is shown below.
  • DTT was added to a final concentration of 50 mM followed by incubation at room temperature for 2 hours.
  • the conjugate was centrifuged, passed through a 0.45 micron filter and purified by chromatography on a 1.5 x 15 cm Sephadex G-10 column equilibrated with PBS.
  • the peptide-Hz-DM conjugate fraction eluted in the column void volume while free daunomycin came off the column considerably later.
  • BSA containing reactive hydrazide groups was prepared as follows. To 250 mg BSA in 34.4 ml of water was added 3 g adipic acid dihydrazide (Sigma) with stirring. The pH was adjusted to 4.75 with 1 N HCl and 90 mg EDC were added. The mixture was allowed to react for one hour at room temperature with stirring while maintaining pH at 4.75. Following reaction, the solution was dialyzed at 4°C against distilled water to remove unreacted dihydrazide and EDC and stored at 4°C.
  • BSA-Hz (0.5 ml, 3.8 mg/ml in 0.2 M sodium acetate buffer pH 6.0) was mixed with 0.187 mg daunomycin in 7.5 ⁇ l of water. The mixture was incubated overnight at room temperature followed by chromatography over a Econo PaclODG column equilibrated with 0.2 M sodium acetate pH 6.0. The BSA-Hz- DM conjugate fraction eluted in the column void volume while free daunomycin came off the column considerably later. The concentration of daunomycin in the conjugate fraction was determined spectrophotometrically. The molecular substitution ratio was 12 molecules of daunomycin per molecule of BSA. b.
  • BSA peptide-Hz-DM was separated from free DM by chromatography over a 1.5 x 15 cm Sephadex G-10 column equilibrated with PBS. The BSA peptide-Hz-DM conjugate fraction eluted in the column void volume while free daunomycin came off the column considerably later.
  • 7G12-BSA peptide -DM conjugate is then purified by chromatography over a 1.5 x 120 cm Sephadex G-50 column equilibrated with PBS. Fractions eluting in the column void column containing protein and daunomycin are collected, pooled and stored at -20°C.
  • a method for modulating wound healing in a tissue of a mammalian host comprising: contacting said tissue with a conjugate comprising a cell proliferation modulating agent and a first member of a specific binding pair, wherein said first member binds with high affinity to a second member of said specific binding pair, and wherein said second member is a binding site for said first member on a component of an extracellular matrix in said tissue.

Abstract

To achieve cellular selectivity of modulation of wound healing, this invention provides conjugates of cell-proliferation-modulating agents and macromolecules that bind with high affinity to extracellular matrix components such as collagen, fibronectin and proteoglycans and/or a specific cell type that migrates into a wound site. These conjugates restrict the proliferation-modulating agent to the wound site and additionally increase the residence time of the conjugate at that site. Use of the conjugate reduces side effects due to the proliferation-modulating agent and reduces frequency of administration.

Description

METHODS AND COMPOSITIONS FOR MODULATION OF WOUND HEALING
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuation-in-part of USSN 08/258,550, filed June 9, 1994, which disclosure is incorporated herein by reference.
INTRODUCTION
Technical Field
This invention relates to methods and compositions for modulating wound healing. The invention is exemplified by a composition comprising a conjugate of a tissue extracellular matrix-specific binding molecule coupled to an antiproliferative or cytotoxic agent for use in inhibition of wound healing in the eye as an adjunct to glaucoma filtering surgery for use in the treatment of proliferative vitreoretinopathy.
Background
Glaucoma encompasses a heterogeneous group of eye diseases characterized by a classical triad of symptoms: elevated intraocular pressure (IOP), optic nerve damage, and progressive visual field loss. These eye disorders affect more than two million Americans and are a leading cause of blindness in the United States. The increase in IOP is due to a decrease in the outflow of aqueous humor, the fluid in the anterior segment of the eye that is responsible for maintaining pressure balance for the entire eye. Glaucomas can be classified according to etiology (primary, secondary or developmental) or to the state of the iridocorneal angle (open or closed). The primary glaucomas are open-angle glaucoma, low-tension glaucoma, and single- closure glaucoma. Secondary glaucomas are the result of a defined local or systemic process which causes elevated IOP, i.e., pigmentary, neovascular, pseudoexfoliative, steroid-induced or lens-induced glaucomas and glaucomas secondary to ocular tumors, trauma, inflammation, hemorrhage and surgery. Developmental glaucomas arise from congenital abnormalities, including developmental anomalies of the anterior chamber angle. The dominant form of glaucoma in the United States and Europe i primary open-angle glaucoma (POAG).
Current medical therapy for glaucoma involves medical, laser and conventional surgical approaches and is directed toward reduction in IOP to a level that is presumed to arrest the progressive damage to the optic nerve. Medical ther of POAG involves treatment with cholinergics (pilocarpine) and/or adrengerics (epinephrine, timolol), as well as oral therapy with carbonic anhydrase inhibitors (acetazolamide). While most glaucoma patients initially respond to drug therapy, many become refractory over time. For those individuals, maintenance of normal IOP requires surgical intervention.
Surgical techniques include laser trabeculoplasty and various types of glaucoma filtering surgery. Laser trabeculoplasty involves the controlled applicatio of intense light to the trabecular meshwork in order to shrink its collagenous tissue and thereby open drainage channels for the aqueous humor. In most patients, laser trabeculoplasty reduces IOP by approximately 25 percent and is often used as an adjunct to medical therapy before resorting to more invasive surgical procedures. However, the initial decreases in IOP observed following trabeculoplasty are often lost after a few months or years.
Glaucoma filtering surgery (GFS) is performed to create a drainage channel for aqueous humor outflow from the anterior chamber in order to lower IOP. Although glaucoma filtering surgery has the potential to replace drug therapy permanently, its current high failure rate makes it an unattractive choice. Uncontrolled scarring of the surgical site usually occurs and the scar tissue frequen blocks the newly created drainage channel. For this reason, GFS is used primarily on "end stage" glaucoma patients who no longer respond to drug therapy and therefore have no other options. Most successful glaucoma filtering surgery results the creation of a filtering bleb, which is an elevation of the conjunctiva at the surgi site. Numerous techniques are sometimes employed as modification to maintain th patency of the drainage fistula, including the use of biocompatible plastic tubes or valves, yet scarring over of the drainage channel frequently causes blockage of the fistula and concomitant increase in IOP. See Shields, Ch. 36, "Filtering Surgery" Textbook of Glaucoma. 3rd ed., Williams and Wilkins, Baltimore, 1992, . Recent clinical studies have demonstrated that introduction into the surgical wound site of agents which inhibit the healing process can improve the success rate of GFS. However, these agents generally are administered by non-specific means such as application by sponge to the drainage filter tissue during the surgical procedure or by repeated, painful injections into the conjunctiva after d e operation. These agents also have undesirable side effects due to their inherent toxicity and the lack of specific localization to the surgical site. Further, the use of antiproliferative agents such as mitomycin C and 5-FU applied directly to the surgical site, often results in inadequate wound healing, leakage of the aqueous humor, hypotony or very low pressure leading to further complications.
Another ophthalmic disease related to harmful proliferation of cells at a surgical site is proliferative vitreoretinopathy (PVR), a potentially blinding disease caused by the escape into and subsequent proliferation of retinal pigmented epithelial cells and other cell types in the vitreous cavity (posterior segment of the eye). This growth and differentiation of cells with the accompanying formation of an epiretinal membrane can eventually lead to a tractional distortion of the retina and eventually detachment of the retina. PVR may occur as a result of accidental or surgical trauma. PVR is currently treated surgically with a procedure called vitrectomy. The operation consists of inserting the tip of a small cutting instrument into the vitreous and removing the cloudy vitreous and scar tissue resulting from the trauma. Due to the delicate nature of the vitrectomy, a typical procedure takes two to three hours to complete. Pharmacological approaches investigated for the treatment or prevention of PVR include steroids and anti-proliferative drugs. These agents are intended to inhibit the initial macrophage-mediated inflammation, which is part of the wound- healing process, in traumatic PVR or to control the proliferation process by killing the respective cell types (RPE, fibroblasts) in a non-specific manner. This experimental therapy has been hindered primarily by non-specific toxicity and the need for multiple administration of the drugs due to their short half-life.
For both glaucoma filtering surgery and proliferative vitreoretinopathy, it would be of interest for the treatment and or prevention of these disorders to identify new methods and compositions to provide localization of agents capable of modulating wound healing at the surgical site. Relevant Literature
The use of colchicine to inhibit cell migration and proliferation in vitro (Lemor, et al. (1986) Arch. Ophthalmol 104:1223) and to treat PVR in an in vivo animal model (Lemor and Glaser (1986) Arch. Ophthalmol. 104: 1226) has been reported. The use of minoxidil (Handa, et al (1983) Invest. Ophthalmol. Vis. Sci. 34:567-575) and retinoic acid have been reported in experimental models of PVR (Ver Straeten, et al. (1990) Invest. Ophthalmol. Vis. Sci. 31: 1761-1766). Hajek, et al ((1986) Invest. Ophthalmol. Vis. Sci. 37: 1010) investigated the pharmacologic inhibition of cell proliferation and migration in vitro. Bi-specific antibodies for use in immunodiagnostic reagents or in the targetin of cytotoxic lymphocytes, drugs or enzymes to specific targets such as cancer cells have been described. See Suresh, et al. (1986) Proc. Natl. Acad. Sci. USA 83:7989 7993; Runge, et at. (1990) Bioconjugate Chem. 1:274-277; Foglesong, et al. (1989) Cancer Immunol. Immunother. 30:177-184); and Milstein and Cuello (1984) Immunol. Today 5:299-304). MacKay, et al (1993) have reported on the construction of a fusion protein with epidermal growth factor (EGF) and the collage binding domain of fibronectin.
The following references may also be relevant to the subject invention: Hudecz et al (1990) Bioconjugate Chem., 1:197-204; Wei-Chiang et al. (1981) Biochem. Biophys. Res. Commun., 102(31:1048-1054: Yang et al. (1988) Proc. Natl Acad. Sci. U.S.A. , £5.: 1189-1193; Wilson et al. (1991) J. of Ocular Pharm., 7(D:1- 8; Xu et al (1993) Ophthal. Surgery, 24(6J:382-388; Tahery et al. (1989) J. of Ocular Pharm. , 5i2): 155-179; Sherwood et al. (1993) J. of Glaucoma, 2:64-67; Jampel et al. (1993) J. of Glaucoma, 2:58-63; Palmer (1991) Ophthalmology, 22:317-321; Miller et al. (1989) Ophthal. Surgery, 2Ω(5J:350-357; European Patent Application 0 253 202 A2; European Patent Application 0 299 467 Al; Miller et al. (1991) Invest. Ophthal. Vis. Sci., 22(4): 1160; Wilkie ev α/. (1991) Am. J. Vet. Res. , V52. N3:441~444: King et al. (1991) American Journal of Veterinary Research, V52 N12:2067-2070: Potter et al. (1988) Journal of Ocular Pharmacology, 4(1):19- 28;Brooks et al. (1990) Small Animal Ophthalmology, 20_:775-797; M. Bruce Shields Textbook of Glaucoma, 3rd ed., 1992., Ch. 34 and 36.
The following references relate to hyaluronic acid (also called hyaluronan): Goetinck et al (1987) /. Cell Biol, 105:2403-2408; Geng et al. (1994) Invest. Ophthalmol & Visual Science, 35:4328-4332; Toole, In Guidebook to the Extracellular Matrix and Adlecien Proteins (eds. Kreis and Vale), pp. 64-65. (1993).
SUMMARY OF THE INVENTION The present invention provides a method and a composition for modulating wound healing in a tissue of interest in a mammalian host. The composition includes a cell proliferation-modulating moiety joined to a targeting moiety that binds with high affinity to a component of the extracellular matrix of the tissue of interest and/or of the plasma membrane of a cell type that is involved in the wound-healing process. The cell proliferation-modulating agent can be either one that inhibits or one that promotes healing of the wound. The method includes the step of introducing the composition into a host, generally in the area of the tissue of interest. The method and compositions find use, for example, in the prevention or treatment of proliferative vitreoretinopathy and in enhancing the success of glaucoma filtering surgery by use of an antiproliferative agent as the cell proliferation-modulating agent to inhibit growth of cells migrating into a wound site.
BRIEF DESCRIPTION OF THE DRAWINGS
Figure 1 shows a schematic representation of one embodiment of the invention wherein a wound in a tissue of interest is contacted with a composition which includes a conjugate between a cell proliferation-modulating agent and a targeting agent that binds with high affinity to a component of the ECM of the tissue of interest. In this embodiment, the cell-proliferation modulating agent is daunomycin attached to the carrier agent albumin, which is bound to the targeting agent which is a monoclonal antibody specific for ECM components. The binding region of the monoclonal antibody binds to a component of the ECM, thus placing the daunomycin in close proximity to the offending fibroblast.
Figure 2 shows a schematic representation of another embodiment of the invention wherein the cell-proliferation modulating agent is a cytotoxic agent attached to the targeting agent, which is an ECM-binding protein. The binding region of the ECM-binding protein binds to a component of the ECM, thus placing the cytotoxic agent in close proximity to the offending fibroblast. Figure 3 shows a schematic representation of another embodiment of the invention wherein the cell-proliferation modulating agent is a cytotoxic agent, a ricin A chain, which is attached to the targeting agent, which is a bispecific antibody with specificity both for ECM components and for fibroblasts. The ECM-specific bindin region of the antibody binds to a component of the ECM, and the fibroblast-specific binding region of the antibody binds to fibroblast so that the cytotoxic agent can be internalized by the fibroblast and kill it.
BRIEF DESCRIPTION OF THE SPECIFIC EMBODIMENTS Methods and compositions are provided for modulating healing of a wound site in a mammalian tissue of interest. The tissue can be any tissue which is characterized by having an extracellular matrix (ECM) composed of one or more components that can serve as a target for the targeting moiety of the conjugate, for example, hyaluronan and polypeptides such as proteoglycans, fibronectin, collagen, certain glycoproteins and the like, and in which migration of particular cell types, such as fibroblasts, macrophages, platelets, polymorphonuclear cells and the like, in the wound site is part of the wound-healing or wound-closure process. Such cell types can also serve as targets. The compositions include a targeting moiety that "targets" a cell proliferation-modulating moiety to one or more components of the ECM and/or cells that migrate into a wound site so that growth of cells migrating into the wound site is either increased or decreased, depending upon the nature of th cell-proliferation modulating moiety. Optionally, a linker agent can be included in the conjugate so that multiple molecules of the proliferation-modulating component can be associated with a single molecule of the targeting moiety. In some embodiments, the targeting moiety of the conjugate is a bispecific agent with combined specificity for cell targets such as fibroblasts and phagocytes and components of the ECM in the tissue of interest.
The composition and method of the subject invention offer several advantages over currently available treatment modalities for glaucoma. Successful filtering surgery can prevent many of the problems associated with medical therapy for
POAG. These problems include patient compliance, ocular and systemic side effects associated with chronic anti-glaucoma therapy and pressure spikes. Current intra- and post-operative medical therapy employs anti-proliferative agents such as 5-fluorouracil (5-FU) and mitomycin C that act nonselectively on all cell types within the anterior segment and ocular adnexa. Diffusion of these agents through tissue is essentially uncontrolled. Extensive diffusion at effective concentrations may be responsible for poor healing of surgical sites, and lead to complications. Limited pharmacokinetic studies have been performed in this area, however, recent reports have suggested that drugs can diffuse through the sclera and be detected in the aqueous humor. Furthermore, 5-FU requires repeated injections (a typical treatment regimen is 1 to 2 injections per day for 14 days) and has a number of undesirable side-effects, which include epithelial and conjunctival ulceration, conjunctiva] wound leaks, retinal toxicity, increased likelihood of ocular infection and pain during administration. With the subject invention, a single application is often sufficient for modulating wound healing following GFS. Furthermore, because the targeting moiety of the conjugate binds with high affinity to a component of the ECM, and/or a cell migrating into the wound site, the growth-modulating component of the conjugate remains localized in the wound site, creating a high local concentration of the growth- modulating agent and minimizing diffusion of the growth-modulating agent into tissue abutting the wound site.
Thus, the subject invention offers advantages which include controlled dosage of the growth-modulating agent and prevention of unwanted effects on essential cells in the tissue of interest: localization of the conjugate to the wound site prevents migration to areas of sensitive cells, especially normal cells, from the wound site and sequesters the growth-modulating agent in the region of invading cells. Another advantage is that it is unnecessary to prepare targeting agents for each tissue of interest, as components of the ECM such as hyaluronan, collagen and fibronectin, are common to many tissues. Specificity is instead achieved by the site of introduction of the conjugate. The resulting effect on the cell type can, as appropriate, include complete elimination of an undesirable cell type from the wound site.
The wound-healing modulating composition is a conjugate comprising a cell proliferation-modulating agent and a targeting agent (first member of a specific binding pair) that binds with high affinity to a component of the extracellular matrix (ECM) of a tissue of interest or at the plasma membrane of a cell involved in healing of the wound. The binding site on the ECM or the cell is the second member of the specific binding pair. By high affinity is intended higher affinity binding to a component of the extracellular matrix or cell surface as compared to other components of the ECM or other cells that may be present in the wound site or in tissue abutting the wound site. In general, where the targeting agent binds to a receptor on the extracellular matrix, the dissociation constant (KJ is about 10"10M to 10^M, preferably less than 10"*M, most preferably less than 10"7M. Where the targeting moiety is an antibody binding to an epitope on the extracellular matrix, th Kj will be on the order of about 10"8M to 10",0M, preferably less than 10"9M. For other targeting agents, such as a peptide binding domain, such as the collagen-bindi domain of fibronectin or the hyaluronic acid binding domains of a number of proteins, i.e., link protein, aggrecan, hyaluronectin, the affinity of the binding domain for the relevant component of the ECM should be sufficient so as to provid specificity of binding to the ECM components.
The targeting agent can be a polyclonal or monoclonal antibody with high affinity for collagen, fibronectin, proteoglycans such as chondroitin sulfate, glycoproteins unique to the extracellular matrix of the tissue of interest, or the like. Where the antibody is a monoclonal antibody, it may be produced as a result of hybridoma formation and expression by the hybridoma, or the supernatant culture, o produced by ascites, or may be a monoclonal antibody fragment, such as FAB, F(ab')2, Fv, a recombinant variable region, or the like. The antibody may be of an mammalian species, including, murine, rabbit, human or the like, or combinations thereof, such as chimeric antibodies, having a human constant region and a mouse o other mammalian source variable region. The important characteristic is that the antibody or antibodies bind with high affinity to one or more components of the extracellular matrix of the tissue of interest. The methods for preparing such antibodies are well established. See, for example, Fazekas de St. Groth et al (1980) J. Immunol. Methods. 35: 1. An anima is hyperimmunized with a suitable immunogen, with or without addition of adjuvant. The source of the immunogen can include specimens such as tissues removed during surgery, biopsy specimens, cultured cell lines and the like. Various components of the extracellular matrix of the tissue of interest or wound-healing cells which migrat into the wound site, such as scleral fibroblasts, macrophages, platelets, and the like may be used as the immunogen, particularly components that are obtainable from th human tissue of interest or human cells where the intended treatment recipient is human, or from another intended recipient animal, where the intended use is a veterinary use or when a conjugate that is cross-reactive with human and animal tissue is desired. Thus, the immunogen can be extracellular matrix obtained from the tissue of interest, for example, human scleral tissue, human conjunctival tissue, human vitreous and human epiretinal membrane. Alternatively, purified components obtainable from the extracellular matrix of the tissue of interest can be used as the immunogen, for example, collagen, fibronectin, proteoglycans, glycoproteins, and the like. Where a particular isotype of a protein is found in the tissue of interest as compared to other tissues, the isotype can be used as the immunogen, for example, where the tissue of interest is human sclera, the immunogen can be type I or in human collagen, where the tissue of interest is vitreous, the immunogen can be type π human collagen.
In accordance with the subject invention, a mammal, which can be a mouse or other mammal, is hyperimmunized with immunogen according to methods well known to those skilled in the art. Once a suitable antibody titer has been obtained in serum of immunized animals, antibody-producing cells such as spleen cells, lymph nodes or lymphocytes from an immunized animal are removed and immortalized using methods well known to those skilled in the art. Hybridomas are prepared and antibodies secreted by the hybridomas are screened to identify the clones that secrete antibodies of the desired specificity. Screening of the hybridoma clones can be by binding to components of the extracellular matrix using an enzyme-linked immunoabsorbant assay (ELISA) or by other screening methods which are known to those skilled in the art, including radioimmunoassay and immunohistochemical staining of frozen sections of the tissue of interest. In addition to screening for binding to components of a particular ECM type, the antibodies also were screened for species specificity and cell type specificity.
For some applications of the subject invention, it may be desirable to use bispecific antibodies rather than monospecific antibodies. By bispecific antibodies is intended antibodies which bind to epitopes on two different substrates, for example they bind both to a component of the ECM and to a component in the plasma membrane of a particular cell type. The bispecific antibodies can be prepared in a number of ways, including linking or combining a monoclonal antibody specific for ECM components of the tissue of interest with another monoclonal antibody specific tor a cellular target, such as a fibroblast cell or a retinal pigmented epithelial (RPE) cell. The two antibodies are joined using, for example, protein A or G. Mixing of different antibodies with protein A can lead either to precipitation or to an ill-defined product, however. In another method, parent hybridoma cell lines each producing a antibody with a desired specificity are fused as described by Milstein and Cuello, (1984) Nature 5:299-304. This technique results in hybridoma cell lines which secrete antibodies of the types produced by each of the parent hybridoma cell lines. With multiple possibilities for heavy and light chain mixing, a heterogeneous population of antibodies can result. However, in some cases the predominant forms are the parental antibodies and a bispecific form.
Fused hybridoma cell lines producing the desired bispecific antibodies can be obtained as described by Fazekas De St. Grogh and Scheidegger (1980) J. Immunol Methods 35: 21-1 and other methods known to those skilled in the art. The hybridoma producing the bispecific antibody can then be grown in large scale culture and purified. Since the parental antibodies typically can have distinctly different isoelectric points, purification is achieved by cation exchange chromatography, for example, on Sepharose fast S (Pharmacia). Fractions from the chromatography can then be analyzed for specificity of binding and purified antibody which binds only to the cells of interest, components of the ECM in the tissue of interest, or both the cell of interest and components of the ECM in the tissue of interest obtained. Examples of bispecific antibodies which have been obtained using this method include the bispecific antibody 1B4, a fusion of hybridomas 3D4 a number of normal epithelial cells including comeal and len epithelial as well as a number of epithelial derived tumor cell lines for example ME-180 cells and 7G12 (vitreous specific; binds to an ECM component of the vitreous) and the bispecific antibody 7B5, a fusion of hybridomas 7H1 (cell specific, binds to a number of normal fibroblast cells derived from various animals for examle human and pig scleral fibroblasts, RPE cells and various cell lines i.e., ME-180 and MRC5) and 7G12 (vitreous specific). Of particular interest are components of the ECM in the vitreous and the sclera. Of particular interest as cell types of interest are scleral fibroblasts, particularly human scleral fibroblasts and RPE cells.
Where the targeting agent is other than an antibody, it can be a protein which binds to extracellular matrix components and includes extracellular matrix binding proteins. Of interest are proteins that bind to hyaluronic acid (HA) (also referred to as hyaluronan), and peptides derived from domains of these proteins which are responsible for binding to HA. Several HA binding proteins have been described including CD44, hyaluronectin/versican, aggrecan and link protein. These proteins share a homologous region containing the HA binding domain. In addition, a novel HA binding sequence unrelated to the above has been described by Hard wick, ((1992) J. Cell Biol. 117: 1343-1350). HA binding domains also have been described for fibronectin and fibrinogen. These peptides can then be linked to drugs directly or by the use of carriers. Equivalent peptides would those peptides which bind HA and that show greater than 50% ssequence homology with known peptides binding HA, i.e., link protein.
Peptides may be prepared using art accepted methods for solid phase syntheses. Peptides prepared with a terminal cysetine or having a terminal control group as described by Winger et al , Biocanjugate Chem. (1995) 6:323-326) can be utilized for direct coupling to appropriately derivatized carrier proteins conjugated to daunomycin. Carrier proteins are derivatized with thiol reactive groups such as N-succinimidyl-32-pyridyldithio propinate (SPDP). Alternatively, peptides may be synthesized such that they have a terminal hydrazide group. Such peptides also can be prepared using conventional methods for solid phase synthesis. Once the final amino acid has been added to the peptide, the terminal amino group is deblocked and treated with succinic anhydride. Succinic anhydride reacts with the amino group to provide a terminal carboxyl group to the peptide. Tert-butyl carbazate (Aldrich) is reacted with this carboxyl group using carbodiimide. An alternative procedure is to react eqimolar amounts of succinic anhydride with tert-butyl carbazate independently. The product of this reaction is then reacted to the deblocked terminal amino group of the peptide using carbodiimide. Final deblocking and decoupling yields the peptide of interest with a terminal hydrazide group. The terminal hydrazide group can then react with the C13 carbonyl group of doxorubicin or daunomycin forming a hydrazone (Yamamoto, et al, (1972) J. Medicinal Chem. 15:872-875). The choice of an agent which provides for modulation of wound healing will depend upon whether inhibition or promotion of wound healing is the desired outcome of treatment. For example, where the intended application is an inhibition of wound healing, specific proliferation-modulating agents include daunomycin, mitomycin C, daunorubicin, doxorubicin, 5-FU, cytosine arabinoside, colchicine, cytochalasin B, bleomycin, vincristine, vinblastine, methotrexate or the like. Also interest are toxic agents which may be derived from microorganism or plant source Examples include the toxic subunits of naturally occurring toxins such as ricin, ab diphtheria toxin, saporin, and the like. Illustrative toxic subunits include the A cha of diphtheria toxin, enzymatically active proteolytic fragments from Pseudomonas aeruginosa exotoxin-A, ricin A-chain, abrin A-chain, modeccin A-chain, and protei having similar activity found in various plants such as the plants Gelonium multiflorum, Phytolacca Americana, Crown, Tiglium, Jatropha, Curcas, Momordic Charantia, Reachan, the toxin saporin from Saponaria qfficinalis (Thorpe et al , (1985) J. National Cancer Institute 75: 151), the Chinese cucumber toxin, trichosanthin (Yeung et al, (1985) Intl. J. of Peptide Protein Res. 27:325-333). Mutant species of the toxins also may be used, for example, CRM 45 (Boquet et al Proc. Natl. Acad. Sci. USA (1976) 73:4449-4453). Where the desired result is enhanced wound healing, the proliferation-modulating agent can be a growth- promoting agent such as fibroblast growth factor, epidermal growth factor, transforming growth factor-j8, and platelet-derived growth factor.
The proliferation-modulating agent and the targeting agent providing for binding to the extracellular matrix can be linked, usually by a bond which is cleavable, either intra- or extracellularly by reduction, hydrolysis, enzymatically, o by a bond which is acid labile. The type of linkage used depends upon a number o factors, but particularly the nature of the proliferation-modulating agent. For example, where the agent is one which must be internalized by a cell to have an effect, such as a toxin molecule or toxin A chain, it is preferable that the linkage to the targeting moiety can be cleaved. The targeting moiety can be linked to a drug either directly or indirectly by carrier molecules such as serum albumin (particularl human serum albumin), polyaminoacids, dextran, and the like, by methods well known to those skilled in the art. The use of a carrier molecule permits binding of multiple molecules of the proliferation-modulating agent to the linker molecule, the number at least partially dependant upon the size of the carrier. Generally, 10 to 3 molecules of agent are bound per molecule of carrier molecule, preferably 5-20, fo an antiproliferative compound, and 1 to 2 molecules per molecule of carrier molecu for a toxin molecule. The type of linkage used can also be dictated by the cell type which is the ultimate goal of the proliferation modulating activity thus preferably the linkage is a pH labile or acid labile link between the targeting moiety and the proliferation- modulating moiety in cases where the cells have phagocytotic properties which migrate into the wound site, for example, fibroblasts and macrophages. Also of interest are peptide linkages which are susceptible to hydrolysis by enzymes which are present in the extracellular matrix. Convenient linkages thus include disulfides, imides, hydrazones, amides and the like.
For some applications it is desirable that the wound-modulating conjugates be associated noncovalently or covalently in an amount sufficient to provide the desired amount of cell with a compound such as hydroypropylemethyl cellulose (HPMC), collagen, destrem, etc. modulation, either cell-proliferation inhibition or enhancement. The conjugate can be incorporated into wafers (or other forms) comprising materials such as HPMC, collagen, dextran, etc. The conjugate would not necessarily be bound to the mixture rather than incorporated with these materials. This is intended to cement and aid the delivery of the conjugate. A wafer could be delivered to a surgical site more efficiently than an injection.
The conjugate can be associated nonconvalently with the polymer either by entrapment of the agent within the biologically inert polymer or by adsorption on to the biologically inert polymer. Generally the rate of release of the growth-modulating agent from the will be at the rate of about 25 to 50% of the amount bound per hour. Generally, the growth-modulating agent is combined with the solid support by assembly of the various components in a sterile environment and the assembly is maintained in an aseptic environment until use. Other agents also can be employed in conjunction with the process of this invention. For example, all-trans retinoic acid can be used to enhance the receptor- mediated cytotoxicity of ricin A-chain immunotoxins, and anti-inflammatory agents can be used. Corticosteroids Name Trade Name
Dexamethazone Maxidex, Decadron, AK-Dex
Fluorometholone Fluor-Op, FML, Flarex
Prednisolone Pred-Forte, AK-Pred, Inflamase
Nonsteroidal
Diclofenac Voltaren
Flurbiprofen Ocufen
Ketorolac Acular
The effectiveness of the conjugate for its intended use may be determined in variety of ways. For example, where the desired effect is inhibition of cell proliferation, particularly proliferation of cells which migrate into an ocular wound, is preferable to test the conjugate using cells as similar as possible to the cell type i the tissue of interest, since the sensitivity of various cell types to growth-modulatin agents may vary significantly. For example, where the intended use of the conjuga is in human ocular tissue, it is preferable that human scleral fibroblast cells obtaine from surgical specimens or fresh eye bank tissues are used; conversely, where the intended use is a veterinary one, it is preferable that the cells be from the intended recipient host species.
Conjugate effectiveness can be evaluated in any of several bioassays includin in vitro cell culture of scleral fibroblasts and an in vivo model of the intended condition for treatment, such as glaucoma or PVR. As an example of an in vitro assay, for determining the ability of the conjugate to inhibit or stimulate cell proliferation in vitro, cell culture plates can be coated with an extracellular matrix component of the type most prevalent in the tissue of interest. As an example, type or type m collagen is prevalent in scleral tissue, type II collagen is prevalent in vitreous. Various concentrations of a conjugate having a high affinity for the ECM component used to coat the plates is then added. The plates are incubated to allow binding of the conjugate to the well associated ECM component, then washed to remove any unbound conjugate. The plates are then washed to remove any unboun conjugate. Culture plates are then seeded with a sub-confluent concentration of cells from the tissue of interest, for example, scleral or conjunctival -derived fibroblasts or RPE cells, and incubated for a sufficient time to observe the desired effect. Growth is determined for each plate by any method known to those skilled in the art, such as total cell count used to differentiate between live and dead cells, trypan blue dye exclusion, tritiated thymidine incorporation and the like. An effective concentration is defined as the dose which inhibits fibroblast proliferation by at least 70%, preferably more than 80%, and most preferably by more than 95% when compared to control plates, i.e., those to which targeting moiety alone is added, or as the dose that stimulates cell proliferation by at least 50%, preferably more than 100% percent, and most preferably by more than 200% when compared to control plates over a period of 24-48 hours.
The conjugates of the subject invention have several uses. In one application of the invention, the tissue(s) of interest are the sclera and/or the conjunctiva of the eye, and the intended application of the invention is to enhance the success rate of glaucoma filtering surgery, in which a drainage fistula or channel has been surgically created to increase aqueous humor outflow as a means of lowering IOP. The intended application of the subject invention in glaucoma filtering surgery is to control healing of the surgical wound such that it heals, but in the process does not create excessive scar tissue to block the filtering channel and bleb that have been made. Since many of the cells in the tissue abutting the wound site are amitotic (non-dividing), the antiproliferative agent will have no effect on these cells, thus conferring additional selectivity to the use of the conjugate.
For evaluation of the efficacy of the invention for use as an adjunct in glaucoma filtering surgery, an in vivo animal model is used. A functioning filter is created in ocular tissue of rabbits according to the methods described by Lee, et al , (1987) Ophthalmol. 94:1523. Pigs, monkeys, dogs, or other animals that provide an art-accepted means for testing glaucoma treatment regimens also can be used as the model animal. Various concentrations of the immunoconjugate are administered to the fistula and bleb areas prior to, during, or following surgery. Eyes are then studied in terms of IOP, outflow facility and percent of eyes with functional filters as determined by ocular examination. At selected times following surgery, animals are humanely sacrificed and the eyes are submitted for histologic evaluation to assess ultrastructural changes in the fistula and filtering bleb. Efficacy of the immunoconjugate is determined by a reduction in IOP, increase in outflow facility and increase in mean duration of time that functional filters are observed when compared to vehicle-treated (control) animals. Another use of the invention is in the prevention of proliferative vitreoretinopathy (PVR). In this disease, cells migrate into the posterior chamber o the eye following accidental or surgical trauma. The clinical presentation will vary depending on whether the condition originated from pre-existing retinal tears (rhegmatogenous retinal detachment) or as a result of other ocular diseases such as diabetes, retinopathy of prematurity or from injury. Retinal detachment has been observed following intracapsular surgery and Nd:YAG laser capsulotomy. Various cell types implicated in the formation of PVR include: retinal pigmented epithelium (RPE), glial cells, fibroblasts, macrophages and blood elements. The usual presentation of PVR is characteristic of the elements of wound healing. Cell type, presence of various growth and differentiation factors, as well as the generation of various extracellular matrix components (ECM) combine to promote the inappropria formation of contractile membranes.
Extracellular matrix components in epiretinal membranes have been analyzed and show collagen and fibronectin to be the major constituents. The preferred anim model for PVR, which can be used to test the efficacy of the conjugate in treating PVR in rabbits, involves compression and partial detachment of the vitreous humor from the retinal surface, followed by introduction of fibroblasts into the posterior chamber of small numbers of cultured fibroblasts (Chandler, et al, (1986) Arch. Clin. Exp. Ophthalmol. 224:86-91). The bispecific or a monospecific conjugate can be used. The experimental animal, for example, a rabbit, is anesthetized and the pupils of the eyes dilated. Cryopexy is performed and seven days following cryopexy, a gas such as perfluorocarbone is injected intravitreally under indirect ophthalmoscopic observation. The gas is left in the eye for about two days after which it is replaced with a balanced salt solution. Seven to ten days after gas-fluid exchange, the eyes are examined for indications of cataract, vitreous hemorrhage, or any retinal detachment. Animals with any sign of these conditions are eliminated from the study. Experimental eyes are injected with cells such as fibroblasts directl above the optic disc. The test conjugate is injected into the posterior chamber of th eye both before, at the time of, or following injection of the fibroblasts. Following injection, animals are monitored for adverse reactions and toxicity using ophthalmoscopic and slit lamp examination of the cornea, anterior chamber, iris and retina Fundus photographs or drawings are obtained at various intervals following fibroblast injection. PVR development is graded using the classification system of Hida, et al, (1987) 225:303-307 Arch. Clin. Exp. Ophthalmol. Eyes are removed from the animals and processed for histological examination and/or immunocytochemical staining to analyze binding of the targeting agent to epiretinal membranes. This model has been used to study daunorubicin (Khawly, et al, (1991) Arch Clin Exp, Ophthalmol, 229: 464-467; Santana, et al , (1984) Arch Clin Exp, Ophthalmol, 221: 210-213; aclacinomycin (Steinhorst (1994) Invest Ophthal Vis Sci, 35(4) 432 and retinoic acid in silicone oil (Araiz, et al (1993) Invest. Ophthal. Vis. Sci. 34:522-530) as treatments of PVR.
In using the subject invention for treatment, a sufficient amount of the growth modulating agent is introduced into the wound site to achieve the desired effect of enhanced wound healing or inhibition of wound healing. Methods for introducing the growth-modulating agent include injection directly into the wound site of a solution comprising the cell-modulating agent. An effective dose of the conjugates for inhibiting wound healing in glaucoma filtering surgery would generally be in the range of 1-500 ug/0.1 ml vehicle, preferably 1-100 ug/0.1 ml vehicle, and most preferably 1-50 ug/0.1 ml vehicle. For treating PVR, an effective dose of the conjugates would generally be in the range of 1-50 ug/0.1 ml vehicle, preferably 1-25 ug/ml vehicle, and most preferably 1-10 ug/0.1 ml vehicle. The vehicle may be any pharmaceutically acceptable solution that is compatible with the conjugates, including phosphate-buffered saline, balanced salt solution and the like. Compatible pharmaceutical stabilizating agents, antiseptic agents and the like may also be used in the conjugate solutions as long as they do not adversely interfere with the activity of the wound-healing-modulating conjugates.
Where the growth modulating agent is used in conjunction with a surgical procedure, it can be introduced before, during or after surgery, preferably at the time of surgery into the wound site. The wound-healing-modulating conjugate may be introduced during the surgery incorporated in a solid support composed of materials including biodegradable polyanhydrides and other biocompatible polymers such as HPMC, dextran or collagen. The wound-healing-modulating agent also can be applied after the surgical incision in the conjunctiva, but before an incision is made the sclera, to allow the agent to bind to the newly exposed target tissue. Alternatively, it may also be applied 1 to 3 days after surgery by injection at or nea the surgical site.
Generally, the affect of the cell-modulating agent associated with the ECM o cell proliferation will be realized within 3 to 24 hours after cells come in contact wi the cell-modulating agent, depending upon the concentration of the agent used and/o the mode of application. In some applications, such as scarring of a fistula site in GFS, it may take from 7 to 30 days. Blockage of the drainage fistula is accompanied by a concomita rise in IOP and loss of bleb patency. These clinical signs of the failure of the fistul and/or the filtering bleb are evaluated by pneumotonometry or other methods and by visual inspection and digital examination of the bleb site. The subject compositions can be provided as kits for use in one or more operations. Kits will include the growth-modulating conjugate either as a concentrate, including lyophilized compositions (which may be further diluted prior use or at the concentration of use, where the vials may include one or more dosages Conveniently, single dosages may be provided in sterilized containers. Where the containers include the formulation for direct use, usually there will be no need for further reagents for use with the method.
The following examples are offered by way of illustration and not by way of limitation.
EXAMPLES
TABLE OF CONTENTS
A. Biological Materials Deposits
B. Examples
1. Preparation of Goat Anti-Collagen/Daunomycin Immunoconj ugates a. Preparation of Goat Anti-Collagen Antibodies b. Preparation of Affinity-Purified Anti-Collagen Antibodies c. Preparation of Anti-Collagen/Daunomycin Immunoconjugates d. In vitro Inhibition of Cell Growth with Anti- Collagen/Daunomycin Immunoconjugates 2. Preparation of Murine Monoclonal Antibodies to Extracellular
Matrix Components
3. Preparation of Murine Monoclonal Antibodies to Ocular Tissues a. Preparation of Antibodies Binding Scleral Fibroblasts and Retinal Pigmented Epithelial Cells b. Preparation of Hybridomas Secreting Bispecific Antibodies 1B4 and 7B5
4. Preparation and Assay of Bispecific Immunotoxins a. Purification of Bispecific Antibody 1B4 b. Conjugation of Ricin A with 1B4 c. Cytotoxic Activity of Bispecific Immunotoxin 1B4-Ricin A 5. Preparation and Assay of 3A3-BSA-DM Immunoconjugates a. Modification of Daunomycin Using Cis-Aconitic Anhydride b. Attachment of Modified Daunomycin to Albumin c. Introduction of Free Sulfhydryl Groups on BSA-DM d. Modification of Antibody 3A3 with SPDP e. Conjugation of 3A3 to BSA-DM f. Cytotoxic Activity of Immunoconjugate 3A3-BSA-DM 6. Preparation and Assay of Fibronectin-BSA-DM Conjugates a. Preparation of Fibronectin-BSA-DM Conjugate b. Cytotoxic Activity of Fibronectin-BSA-DM 7. Effect of Fibronectin-BSA-DM on IOP after Filtering Surgery in Normotensive Rabbits
8. Effect of 3A3-BSA-DM Conjugate on IOP after Filtering Surgery in Beagles with Glaucoma
9. Effect of 1B4-RA Immunoconjugates on Development of PVR after Vitrectomy in Rabbits 10. Conjugation of Daunomycin to Carrier Proteins: Preparation of
DM-BSA-DPDPB
11. Conjugation of DM-BSA-DPDPB to 7G12 Fab' 12. Conjugation of DM-BSA-DPDPB to the Collagen Binding
Domain of Fibronectin
13. Conjugation of DM-BSA-DPDPB to Hyaluronic Acid Binding Peptides (HABP)
14. Conjugation of Daunomycin to Hyaluronic Acid Binding Peptides Containing a Terminal Hydrazide Group
15. Conjugation of Daunomycin to BSA-Hydrazide
16. Conjugation of 7G12 to BSA Peptide-Hz-DM
Biological Materials Deposits The following hybridomas, which secrete monoclonal antibodies described below, were deposited on June 8, 1994, with the ATCC under the indicated accessi numbers:
Hybridoma ATCC Accession Number
7G12 6G10
7H1
1B4
7B5 Example 1
Preparation of Goat Anti-Collagen/Daunomycin
Immunoconjugates a. Preparation of Goat Anti-Collagen Antibodies. A castrated male Spanish goat, 3-5 years of age, was immunized with type I human collagen (Calbiochem) emulsified in Freund's complete adjuvant (Difco). The animal was given 50 ug collagen in adjuvant as 6-8 subcutaneous injections (prescapular, post-scapular and lateral lumbar regions) at 0.1 - 0.2 ml per site with a total injection volume of 1.0 ml. Booster injections were given every two weeks for six weeks. Antibodies to type I collagen were detected by ELISA, d e animals were bled and serum obtained by conventional methods. b. Preparation of Affinitv-Purified Anti-Collagen Antibodies.
A purified immunoglobulin (IgG) fraction was prepared from immune goat serum by ion exchange chromatography. Serum (360 ml) was diluted ten fold in 50 Mm MES buffer, pH 6.0 and clarified by passage over approximately 800 ml of Cell Debris Remover (CDR) (Whatman) using a Buchner funnel. The diluted and clarified serum was applied to a Fast S (Pharmacia) cation exchange column (100 ml bed volume) equibrated in the same buffer used to dilute the serum. The bound IgG fraction was eluted with 50 Mm MES pH 6.0 containing 200 Mm NaCl and dialyzed overnight against phosphate-buffered saline (PBS) at 4°C. The purified immunoglobulin fraction was applied to a 20 ml gelatin-Sepharose 4B column (Pharmacia). Bound antibody was eluted with 200 Mm glycine-Hcl buffer pH 2.5. Affinity purified antibody (40 ml) was dialyzed overnight against 50 Mm MES buffer pH 6.0 and passed over a 1.5 ml Fast S column equilibrated in the same buffer. Bound antibody was eluted with 50 Mm MES pH 6.0 containing 200 Mm NaCl. The antibody solution was desalted into PBS using a Econo Pac 10 DG column (Bio-Rad). Protein concentration was determined by absorbance at 280 nm. Six ml of affinity- purified antibody at 1.3 mg/ml was obtained. c. Preparation of Anti-Collagen Daunomvcin Immunoconjugates. Affinity-purified goat anti-type I collagen is conjugated to daunomycin by a method modified from Yang et al. (1988) Proc. Natl. Acad. Sci. , 25: 1189-1193. Briefly, 5 mg of cis-aconitic anhydride (Sigma) is dissolved in 0.050 ml dioxane and added dropwise to an ice-cold solution of daunomycin (DM) (10 mg in 1.0 ml 400 Mm sodium bicarbonate buffer ph 9.0). The pH is maintained at 9.0 with the addition of 0.5N NaOH. After 15 min., the pH is adjusted to 7 and 0.4 ml of 1 Hepes buffer added. After incubation for one hour at room temperature, six mg o ethyl-3-(3-dimethylaminopropyl-carbodiimide (Sigma) and six mg N- hydroxysulfosuccmimide (Pierce) is added to the reaction mixture. Six mg of affin purified antibody (10 mg/ml) in 50 Mm Hepes buffer pH 7.0 is added and the reaction allowed to proceed overnight at room temperature. The immunoconjugate purified by passage over a Econo Pac 10 DG desalting column equilibrated with P The concentration of DM is determined by measuring the absorbance at 476 nm (extinction coefficient of 17.3 at lmg/ml). The IgG concentration is determined using the Bradford Coommassie Blue dye binding assay (Bio-Rad). d. In vitro Inhibition of Cell Growth with Anti-Collagen Daunomycin Immunoconjugates. The anti-type I collagen-DM conjugate cytotoxicity can be tested on human rabbit scleral derived fibroblasts. Briefly, 24 well culture plates are precoated with rat tail collagen (Collagen Corp.) 0.2 ml/well, 200 ug/ml and the collagen allowed dry overnight at room temperature. The following day additional wells are loaded with 1.0 ml PBS containing 0.1% BSA and the plates are incubated for an addition 2 hours at 37°C. Wells are washed twice with PBS to remove unbound protein. Dilutions of immunoconjugate in PBS containing 0.1 % bovine serum albumin (BS are prepared and added to wells. PBS containing 0.1 % BSA was added to collage and BSA-treated wells as controls. The plates are incubated at 37° for 3 hours to allow for immunoconjugate binding, then washed twice with PBS to remove unbou material. A suspension of human scleral fibroblasts in M199 media (Gibco) containing 10% fetal bovine serum (FBS) is added to each well (1.0 ml/well at 1 X 104 cells/ml). Plates are incubated at 37° for 48 hours followed by the addition of 2.0 uCi 3H-thymidine (Amersham) and incubation for 24 hours. Following thymidine incorporation, cell monolayers are rinsed three times with PBS and 1.0 5% ice cold trichloroacetic acid (TCA) added to the wells. After 30 minutes on ic the well are rinsed once with 5% TCA and the precipitate dissolved by the addition of 1.0 ml 0.1 N NaOH containing 2% Na2CO3 and incubation at 37°C for one hou Following neutralization of the samples by the addition of 15 ul 12 N Hcl/well, the amount of radioactivity in each well is determined by adding 0.5 ml of each sample to 9.0 ml scintillation fluid and counting in a Beckman scintillation counter.
Example 2 Preparation of Murine Monoclonal Antibodies to
Extracellular Matrix Components Preparation of hybridomas secreting monoclonal antibodies to extracellular matrix components follows standard procedures developed in a number of laboratories (Fazekas de St. Groth et al. (1980) J. Immunol. Methods, 35:1-21; and Goding, Monoclonal Antibodies: Principles and Practice, Academic Press, London, 1986). Individual female BALB/c mice, (4 to 6 weeks of age) were immunized with human scleral tissue (homogenized in PBS), bovine plasma fibronectin (pFN) (purified from bovine plasma by gelatin-sepharose affinity chromatography) and human vitreous. Antigen preparations were emulsified in Freunds complete adjuvant (Difco). Mice were given 50-100 ug of antigen preparation injected subcutaneously. Booster injections were prepared in Freunds incomplete adjuvant. Three days prior to fusion mice were given a final boost of antigen in PBS given as an intraperitoneal injection.
One day prior to cell fusion, peritoneal macrophage feeder cells were prepared. Five BALB/c mice were sacrificed by CO-, asphyxiation and their peritoneal cavities flushed with 5 ml sucrose solution (0.34M). Washings were pooled, counted and centrifuged. Cells were resuspended in Iscoves-modified Dulbeccos containing hypoxanthine, aminopterin and thymidine (IMDM) media to a concentration of 6 X 10* cells/ml. The cells were then distributed in ten 96 well plates at 100 ul/well and incubated at 37°C in a CO2 incubator. The P3-X63/AG8.653 myeloma cell line (ATCC # CRL 1580) was grown in
IMDM containing 10% FBS. The cells were maintained in log phase growth at low density and split 1:2 the day before the fusion. On the day of fusion myeloma, cells were collected and washed in IMDM media with 10% serum. The final wash and resuspension was in IMDM without serum. Cells were suspended to a concentration of 1 X107 cell ml. All procedures were performed at room temperature.
The spleen from each immune mouse was removed aseptically and minced to a single cell suspension. Cells were suspended in 10 ml IMDM and large clumps allowed to settle for 10 minutes. Nine ml of cell suspension were transferred to a 50 ml centrifuge tube, made up to 50 ml with IMDM and cells counted with hemocytometer. Cells were then centrifuged at 200 X g for 10 min and suspended 1 X 107 cells/ml in IMDM. The preparation of myeloma and spleen cells was carri out simultaneously. For fusion, 10 ml of myeloma and 6 ml of spleen cells were combined with
34 ml IMDM and centrifuged at 200 X g for 10 min. The supernatant was remove and the pellet loosened by gentle agitation. One ml of 45% PEG 4000 (Gibco) was added dropwise over one minute with constant agitation of the tube. The tube was then immersed for 90 seconds in a 37°C water bath with gentle agitation and finally centrifuged at 20 X g for 1 min. Fusion was stopped by slowly diluting PEG with IMDM. Twenty ml IMDM was added, 1 ml over the first 30 seconds, 3 ml over th next 30 seconds, and the rest over the second minute. The cells were then allowed stand for 5 minutes, centrifuged for 10 minutes at 200 X g, and the supernatant discarded. Following fusion, cells were suspended in IMDM media containing hypoxanthine, aminopterin and thymidine (HAT) and 20% FBS and plated on the previously prepared macrophage feeder cells to give approximately 1 X 104 cell/well Cells were refed on days 5, 12 and 15, with half (100 μl) of the media removed and replaced. When the positive cultures were identified by screening assays (see below they were transferred to 24 well plates containing 1 ml of IMDM media containing hypoxanthine and thymidine and 10% FBS. When confluent, the expanded cultures also were retested and positive cultures cloned, frozen and expanded. The expanded cultures were also tested and frozen. Further expansion and cloning was performed in media without hypoxanthine, aminopterin or thymidine. Culture supernatants were tested for specific monoclonal antibodies by ELIS using antigen coated plates. Results of hybridomas selected from each fusion are shown in Table 1. Table 1
Binding of Hybridoma Culture Supernatants to ECM Antigens by ELISA1
Scleral
Supernatant Vitreous2 pFN3 Extract
7G12 0.75 0.08 1.2
3A3 0.10 1.2 0.13
6G10 0.01 0.02 1.0 'ELISA assays were performed in 96-well polystyrene test plates. Antibodies were detected using goat anti-mouse IgG coupled to peroxidase (Kirkegaard and Perry). ABTS was used as the substrate. Absorbance readings were determined with a Dynatech plate reader at 410 nm. 2Rabbit vitreous diluted in PBS and dried overnight at room temperature. 'Bovine plasma fibronectin diluted in PBS and incubated overnight at 4βC. 'Human sclera tissue extracted in PBS using a Dounce homogenizer and dried overnight at room temperature.
Supernatants from hybridomas 7G12 and 6G10, that tested positive for human scleral tissue, were also examined for antibody binding to human type I collagen. Results are shown in Table 2.
Rbje_2 Binding of Hybridoma Culture Supernatants to Human Type I Collagen by ELISA1
Supernatant Tvpe I Collagen BSA
7G12 0.16 0.03
6G10 0.40 0.00 'The assay was performed in a 96-well polystyrene plate. Human type I collagen
(Sigma) diluted in M199, added to wells and dried. Additional wells were treated with BSA diluted in PBS. Antibodies were detected with goat anti-mouse IgG coupled to peroxidase. ABTS was used as the substrate. Results are expressed as absorbance at 410 nm and are the average of duplicate samples.
Production of the Ascities fluid containing monoclonal antibodies and high concentration was performed as described by
Harlow and Lane (Antibodies: A Laboratory Manual, Cold Spring
Harbor Laboratories (1988) Cold Spring Harbor, New York). Hybridomas cells were cultivated in cell culture media, harvested by centrifugation and washed PBS. Cells (1-5 X 106) were injected intraperitoneally into adult females BOBA/C mice. Mice had been primed by injection of 0.2ml 2,6,10,14-teιtramethyl decanoic acid (Pristane, Sigma) ten to 14 days prior to injection of cells. Ascities fluid was harvested from the mice within three weeks following injection of cells. The Ascities food was centrifuged at 3,000 x g for ten minutes, cooled and stored at -20CC.
Example 3
Preparation of Murine Monoclonal Antibodies to Ocular Tissues a. Preparation of Antibodies Binding Scleral Fibroblasts and Retinal Pigmented Epithelial Cells. Preparation of hybridomas secreting monoclonal antibodies to scleral fibroblasts and retinal pigmented epithelial cells followed standard procedures developed in a number of laboratories (Fazekas de St. Groth (1980); and Goding (1986). Individual BALB/c mice were given interperitoneally human scleral fibroblasts in PBS. Subsequent injections were given intravenously. Three days prior to fusion, mice were given a final boost of cells by an intravenous injection. Fusion procedures using P3-X63/AG8.653 myeloma were performed as in Example 2. Culture supernatants were tested for antibodies binding human scleral fibroblasts by ELISA. Positive cultures were expanded, cloned by limiting dilution and frozen in liquid nitrogen. Results of binding assay using culture supernatant from one positive hybridoma (7H1) to various cell types are given in Table 3. Results of binding using culture supernatant from hybridoma 3D4 (US Patent No. 5,055,291) is also given. b. Preparation of hybridomas secreting bispecific antibodies 1B4 and 7B5. Hybridomas producing bispecific antibodies were produced as described by Wong et al (1987) J. Immunol. 139:1369-1374. This procedure results in hybridoma cell lines secreting both types of antibody. With multiple possibilities Table 3 Binding of Monoclonal Antibodies to Vitreous and Cells by ELISA1
Vitreous2 ME1803 HSF4 RSF5 PSF6 RPE7 MRC58
Parental
7H1 0.01 0.77 0.32 0.08 0.30 0.59 0.87
3D4 0.01 0.75 0.02 0.22 0.04 0.13 0.02
7G12 0.54 0.02 0.02 0.08 NT 0.25 0.12
Bispecific
1B4 0.86 0.21 NT
10 7B5 0.64 0.14 0.52
'ELISA assays were performed in 96-well polystyrene test plates. Individual cell suspensions were added to wells in M199 containing 10% FBS and allowed to
NJ attach by overnight incubation at 37 βC. Sufficient cells were added to give complete confluence at the time of assay. Bound monoclonal antibodies were detected using goat anti-mouse IgG coupled to peroxidase (Kirkegaard and Perry). ABTS was used as the substrate. Absorbance readings were determined with a Dynatech
15 plate reader at 410 nm.
2Pig vitreous diluted in PBS and dried overnight at room temperature.
'ME 180, human cervical carcinoma cell line ATCC Number HTB33. HSF= human scleral fibroblast
SRSF= rabbit scleral fibroblast
20 *PSF=pig scleral fibroblast
7RPE=pig retinal pigmented epithelial cells
*MRC5, human fibroblast cell line ATCC Number CCL171.
NT = Not Tested
Figure imgf000029_0001
for heavy and light chain mixing a heterogeneous population of antibodies can result. However, in some cases the predominant forms are parental antibodies and a bispecific antibody (Milstein et al. (1984) Nature, 305:537-540). 7G12 cells secreting a monoclonal antibody binding vitreous were made thymidine kinase deficient (TK-) by incubation in IMDM containing 10% FBS and an increasing concentration of 5-bromo-2'-deoxyuridine (1.95 x 10-5 to 1.95 x 10-4 M). 3D4 and 7H1 cells (secreting antibodies binding cells as described above) were made hypoxanthine guanine phosphoribosyl transferase deficient (HGPRT-) by incubation in culture medium containing 8-azaguanine (3.3 x 10-5 M). Following incubation in respective media surviving cells were subcloned by limiting dilution (0.5 cells/ well) in 96 well culture plates and the subclones tested for viability in medium containing hypoxanthine, aminopterin and thymidine (HAT). Subclones sensitive to HAT and producing antibody were selected for fusion. TK- 7G12 cells were fused with HGRPT- 3D4 and 7H1 cells as described in Example 2. Culture supernatants were tested for binding to both vitreous and fibroblasts or ME180 cells using procedures described above. A hybridoma culture testing positive for antibody binding to vitreous and ME180 cells (7G12X3D4 fusion) was labeled as 1B4. A hybridoma culture testing positive for binding to vitreous and fibroblasts (7G12X7H1 fusion) was labeled as 7B5. Results of ELISA assays for parental and bispecific antibodies to their respective antigens is shown above in Table 3. Binding by parental 7G12 supernatant from Example 2 is also shown.
Example 4 Preparation and Assay of Bispecific Immunotoxins a. Pyrification of Pjspecjfic ntibody 1P4.
1B4 hybridoma cells, prepared as described in Example 3, were grown in IMEM containing 10% FBS in spinner culture. Three liters of culture supernatant were obtained and clarified by passage over CDR, concentrated to 500 ml by ultrafiltration, diluted 1:10 with 50 Mm MES buffer pH 5.6 and applied to a Fast S cation exchange column. 1B4 antibody was batch eluted with MES buffer containing NaCl. The column was first treated with buffer containing 125 Mm NaCl. Parental 3D4 antibody eluted at this concentration. 1B4 was then eluted with MES buffer containing 175 Mm NaCl. Parental 7G12 antibody was eluted with 250 Mm NaCl in MES buffer. The eluted bispecific antibody was further purified over protein A-Sepharose (Pharmacia) and the eluted antibody desalted over an Econo Pac 10 DG column equilibrated with PBS.
b. Conjugation of Ricin A with 1B4.
Purified 1B4 antibody was treated with a 7 fold molar excess of SPDP (1 mg/ml in ethanol) incubated for 1 hour at room temperature and desalted over a Econo Pac 10 DG column equilibrated with PBS. SPDP derivitized 1B4 was mixed with ricin A and incubated for 2 hours at room temperature followed by 4°C overnight.
c. Cytotoxic Activity of Bispecific I munotoxin 1B4-Ricin A.
Cytotoxic activity of 1B4-RA was tested essentially as described in Example Id. Briefly, 24-well culture plates were precoated with rabbit vitreous diluted in PBS (0.2 ml/well, 6.2 ug/ml of protein) and dried overnight at room temperature. The following day wells were fixed by the addition of absolute ethanol for 30 minutes at 4°C and rinsed with M199 containing 10% FBS. Additional wells were loaded with 1.0 ml PBS containing 1.0 % BSA and the plates were incubated for an additional hour at 37°C. Wells were washed twice with M199 containing 10% FBS. Dilutions of 1B4-RA in M199 containing 10% FBS were prepared (250 ul/well at 250 ug/ml) and added to triplicate wells. M199 containing 10% FBS was added to triplicate wells as a control and the plate incubated at room temperature for 2 hours to allow for immunotoxin binding, then washed three times with PBS to remove unbound material. A suspension of ME180 cells in M199 containing 10% FBS was added to each well (2 X 104 cells in 1.0 ml/well). Plates were incubated at 37°C for 48 hours followed by the addition of 2 Uci per well 3H-leucine (Amersham) and incubation for 24 hours. Following leucine incorporation, cell monolayers were processed as in Example Id and the amount of radioactivity in each well was determined by counting in a scintillation counter. Results are show in Table 4. Figure 3 shows a schematic representation of the bispecific immunotoxin. Table 4
Cytotoxic Activity of Culture Wells Coated with
Vitreous and Treated with 1B4-RA for ME180 Cells
3H Leucine Incorporation fcpm1)
Culture wells coated with: Vitreous BSA
Wells treated with:
Media (control) 15,511 12,262
IB4-RA 3,272 18,211 'individual culture wells were coated with a solution of rabbit vitreous diluted in PBS and then treated with 1B4-RA. Following incubation to allow for immunotoxin binding, the wells were rinsed with PBS to remove unbound conjugate and ME 180 cells in M199 containing 10% FBS were added. Following incubation, the ability of cells to incorporate 3H-leucine was determined.
Example 5 Preparation and Assay of 3A3-BSA-DM Immunoconjugates a. Modification of Daunomycin using Cis-Aconitic Anhydride. Thirty mg of daunomycin (Sigma Chemical Co.. St. Louis MO) was dissolved in 1.8 ml of ice cold water. Bicarbonate buffer (1.2 ml of a 1M solution, pH 9.0) was added and the solution was placed on ice with stirring. A solution of 0.15 ml dioxane containing 15 mg of cis-aconitic anhydride was added dropwise. The pH was maintained at 9.0 by the addition of 0.5 N NaOH. The reaction mixture was incubated for 15 minutes at 4°C with stirring. Following incubation, 1.2 ml of 1.0 M Hepes buffer, pH 7.0 was added and the reaction mixture was removed from the ice bath and incubated for 60 minutes. Following incubation 155 mg of emyl-3-(3-dime ylaminopropyl)carbodiimide-Hcl (Pierce) and 150 mg N-hydroxysulfosuccinimide (Pierce) was added to the daunomycin solution and dissolved. The solution was allowed to incubate for 10 minutes and the final volume adjusted to 4.8 ml by the addition of 0.3 ml of 1 M Hepes buffer. b. Attachment of Modified Daunomycin to Albumin.
Bovine serum albumin (Sigma Chemical Company) was dissolved in 0.1 M MES buffer, pH 6.0. Twenty mg BSA in 1.12 ml was added to 3.2 ml of the daunomycin solution prepared above with stirring. The reaction vial was incubated overnight at room temperature in the dark to give BSA-DM. c. Introduction of Free Sulfhydryl Groups on BSA-DM.
Following overnight incubation BSA-DM was separated from free DM by desalting over a Econo Pac 10 DG column equilibrated with 50 Mm MES buffer pH 6.0. Limited reduction of disulfide bonds in the BSA-DM was performed by adding dithiothretiol (DTT) to a final concentration of 50 Mm followed by incubation at room temperature for 30 minutes. DTT was removed by desalting over a Econo Pac 10 DG column equilibrated with PBS containing 1 Mm EDTA. As a final step the BSA-DM solution was concentrated to a final volume of 1.1 ml by ultrafiltration (molecular weight cutoff of 10,000 daltons) using a Centricon 10 (Amicon). A ratio of 22: 1 DM molecules per BSA molecule was obtained. d. Modification of Antibody 3A3 with SPDP.
Purified 3 A3 antibody (0.83 mg/ml in PBS) which binds fibronectin was treated with a 7.5 molar excess of long chain (LC)-SPDP (Pierce) (lmg/ml (LC)- SPDP in ethanol) and incubated at room temperature for 1 hour. e. Conjugation of 3A3 to BSA-DM.
3A3-(LC)-SPDP (0.9 ml) was added to 0.250 BSA-DM prepared from Example 5c and incubated for one hour at room temperature followed by overnight incubation at 4°C. One ml of this mixture was diluted to 10 ml with 50 Mm MES pH 6.0 and applied to a Fast S cation exchange column (1.0 ml bed volume) equilibrated in the same buffer. Unconjugated BSA-DM did not bind to the column. The column was rinsed with starting buffer and bound immunoconjugate was eluted with 20 Mm phosphate buffer containing 300 Mm NaCl pH 7.2. The 3A3-BSA-DM immunoconjugate was collected in a 1.2 ml fraction, f. Cytotoxic Activity pf Immunoconjugate 3A3-BSA-PM. Bovine fibronectin (CalBiochem) was diluted to 50 ug/ml in PBS and 0.2 ml aliquots were added to wells of a 24 well culture plate and incubated overnight at 4°C. Following incubation wells were rinsed twice with PBS to remove unbound protein. One ml of 3A3-BSA-DM was diluted 1:3 in PBS containing 0.1 % BSA and filter sterilized with a 0.45 micron filter and added to wells previously coated fibronectin. Control wells were loaded with PBS containing 0.1 % BSA. Following incubation for three hours at 37°C the wells were rinsed with PBS. One ml of MRC-5 fibroblasts (ATCC No. CCL 171)(1 X 104 cells/ml) in M199 containing 10% FBS was added to each well. 3H-thymidine was added and the wells processed as described in Example Id. Results are shown in Table 5.
Table 5 Cytotoxicity of 3A3-BSA-DM for MRC-5 Fibroblasts
Incorporation of SH-Thvmidine1 3A3-BSA-DM 30,006
0.1 % BSA in PBS 107,465 'individual culture wells were coated with solutions of fibronectin and then treated with 3A3-BSA-DM and PBS containing 0.1 % BSA. Following incubation to allow for immunoconjugate binding, the wells were rinsed with PBS to remove unbound conjugate and MRC-5 fibroblasts in M199 containing 10% FBS were added. Following incubation, the ability of cells to incorporated 3H-thymidine was determined. Data represent average of triplicate samples.
Example 6 Preparation and Assay of Fibronectin-BSA-DM Conjugate a. Preparation of Fibronectin-BSA-DM Conjugate.
DM-BSA with free sulfhydryl groups was prepared as described in Example 5. Bovine plasma fibronectin (0.9 ml) (Calbiochem) at 1.0 mg/ml in PBS was treated with a 5-fold molar excess of (LC)-SPDP (1 mg/ml in ethanol) and incubated for 1 hour at room temperature. (LC)-SPDP-fibronectin (0.9 ml) was mixed with 0.25 ml BSA-DM and incubated for one hour at room temperature followed by overnight incubation at 4CC to produce Fibronectin-BSA-DM conjugate, b. Cvtotoxic Activity of Fibronectin-BSA-DM.
Gelatin (Sigma) was diluted to 100 μg/ml in water and 0.2 ml aliquots were added to wells of a 24 cell culture plate and allowed to dry overnight at room temperature. Following incubation, wells were rinsed twice with PBS to remove unbound material.
Following incubation, 0.5 ml of the Fibronectin-BSA-DM conjugate was diluted to a fibronectin concentration of 50 ug/ml in PBS containing 0.1 % BSA and added to wells previously coated with gelatin. Control wells were loaded with PBS containing 0.1 % BSA. Following incubation for three hours at 37°C the wells were rinsed with PBS. One ml of MRC-5 fibroblasts (1 X 10* cells/ml) in M199 containing 10% FBS was added to each well. After incubation for 48 hours, 3H-thymidine was added and the wells processed as described in Example Id. Results are shown in Table 6. Figure 2 shows a generic schematic representation of the Fibronectin-BSA-DM conjugate.
Table 6 Cytotoxicity of 3A3-BSA-DM for MRC-5 Fibroblasts
Incorporation of SH-Thvmidine1 Fibronectin-BSA-DM 12,255 cpm
0.1% BSA in PBS 135,198 cpm
'individual culture wells were coated with solutions of gelatin and then treated with Fibronectin-BSA-DM or PBS containing 0.1 % BSA. Following incubation to allow for the conjugate to bind, the wells were rinsed with PBS to remove unbound conjugate and MRC-S fibroblasts in M199 containing 10% FBS were added. Following incubation, the ability of cells to incorporated 3H-thymidine was determined. Data represent average of triplicate samples.
Example 7
Effect of Fibronectin-BSA-DM on IOP after Filtering
Surgery in Normotensive Rabbits
The creation of a functioning filtering bleb in six 2.0-2.5 kg rabbits is carried out according to the methods described by Lee, et al (Ophthalmol.
94:1523, 1987). The animals receive surgery in both eyes, with one eye treated with Fibronectin-BSA-DM conjugate and the fellow eye treated with vehicle control. Rabbits will be given a preoperative eye examination with a Zeiss slit lamp biomicroscope. Preoperative IOP will be obtained from the average of three measurements by pneunototometry, using an Applanation Pneumatograph
(BioRad), after the installation of one drop of 0.5% proparacaine Hcl to each eye. Each rabbit receives a subconjunctival injection at the operative site in one eye of 25 ug conjugate in 0.1 ml PBS two hours prior to surgery. The other eye receives a similar injection of 0.1 ml PBS as a control. The rabbit is placed under general anesthesia, a lid speculum placed, a limbal-based conjunctival portame is made approximately 8 mm posterior to the li bus and sharp and blunt dissection performed until the cornea scleral limbus is well visualized. A triangular partial thickness scleral flap is then developed based at the limbus to approximately 50% scleral depth, and then an entry wound into the anterior chamber is made with a sharp 15 degree razor knife. A 1 mm x 3 mm sclerostomy is then performed to excise the tissue under the partial thickness flap. A peripheral iridectomy is performed with Vannus scissors and curved jewelers' forceps. The flap is sutured in place with 10-0 nylon suture and the conjunctiva closed with a running absorbable suture.
The animals are then given a combination antibiotic/steroid ointment applied to each eye, are kept warm, and observed every hour for eight hours and then every four hours the following day. At 24 and 72 hours post-surgery, the rabbits are given subconjunctival injections in both eyes as above. Daily observations continue thereafter with the instillation of the antibiotic steroid ointment daily for approximately 21 days. Examinations including a routine ophthalmic examination for bleb patency, toxicity and complications, slit lamp biomicroscopy and pneumotonometry, are performed daily for the first five days after surgery, then every third day for eight weeks. Observations, including variations in IOP, are subjected to standard statistical analysis to look for maintenance of lowered IOP.
Example 8 Effect of 3A3-BSA-DM Conjugate on IOP after Filtering Surgery in Beagles with Glaucoma Beagle dogs are naturally susceptible to glaucoma and are well accepted as a model for human glaucoma (King et al. (1991) Am. J. Vet. Res. 52:2067-2070). Two groups of six beagles each with glaucoma, as characterized by IOP greater than 30 mm Hg in one or both eyes receive glaucoma filtering surgery in one glaucomatous eye; one group receives 3A3-BSA-DM conjugate and the other group is treated with vehicle control. Beagles will be given a preoperative eye examination with a Zeiss slit lamp biomicroscope. Preoperative IOP will be obtained from the average of three measurements by pneunototometry, using an Applanation Pneumatograph (BioRad), after the installation of one drop of 0.5% proparacaine HC1 to each eye. Each dog receives a subconjunctival injection at the operative site in one eye of 25 ug conjugate in 0.1 ml PBS two hours prior to surgery. The other eye receives a similar injection of 0.1 ml PBS as a control. The dog is placed under general anesthesia, a lid speculum placed, a limbal-based conjunctival portame is made approximately 8 mm posterior to the limbus and sharp and blunt dissection performed until the cornea scleral limbus is well visualized. A triangular partial thickness scleral flap is then developed based at the limbus to approximately 50% scleral depth, and then an entry wound into the anterior chamber is made with a sharp 15 degree razor knife. A 1 mm x 3 mm sclerostomy is then performed to excise the tissue under the partial thickness flap. A peripheral iridectomy is performed with Vannus scissors and curved jewelers' forceps. The flap is sutured in place with 10-0 nylon suture and the conjunctiva closed with a running absorbable suture.
The animals are then given a combination antibiotic/steroid ointment applied to each eye, are kept warm, and observed every hour for eight hours and then every four hours the following day. At 24 and 72 hours post-surgery, the beagles are given subconjunctival injections in both eyes as above. Daily observations continue thereafter with the instillation of the antibiotic steroid ointment daily for approximately 21 days. Examinations including a routine ophthalmic examination for bleb patency, toxicity and complications, slit lamp biomicroscopy and pneumotonometry, are performed daily for the first five days after surgery, then every third day for eight weeks. Observations, including variations in IOP, are subjected to standard statistical analysis to look for maintenance of lowered IOP.
Example 9
Effect of 1B4-RA Immunoconjugate on Development of PVR after
Vitrectomy in Rabbits The refined experimental PVR model involving vitreous gas compression and gas-fluid exchange as described by Chandler et al , (1986) is used to test in vivo activity efficacy of 1B4-RA immunotoxin in rabbits; i.e., to test the ability of the compound to bind in vivo as well as its ability to exert a cytotoxic effect in vivo. Two groups of six Dutch-Belted rabbits each weighing 1.5-2.0 kg are used in the study. All rabbits undergo vitreous gas compression and gas-fluid exchange, and one group receives the immunotoxin, while the other receives a vehicle control. Before each step in the procedure, rabbits are anesthetized with intramuscular injections of ketamine Hcl (30 mg/kg) and eylazine (5 mg/kg). The pupils of experimental eyes are dilated with phenylephrine (5.0%) and tropicamide (0.25%). Briefly, cryopexy is performed at approximately 4 mm from the limbus at the lower nasal quadrant. Seven to 10 days following cryopexy, 0.3 ml of perfluorocarbone gas is injected intravitreally with a 30-gauge tuberculin syringe in two portions of 0.2 ml and 0.1 ml. Injections are performed under indirect ophthalmoscopic observation. The gas is left in the eye for 2 days after which it is replaced with BSS. Seven to 10 days after gas fluid exchange, eyes are examined for indications of cataract, vitreous hemorrhage, or any detachment. Animals with any sign of these conditions are eliminated from the study. Experimental eyes are injected with 25,000 fibroblasts directly above the optic disc. Cultured rabbit fibroblasts (described above) are harvested from cell culture by trypsinization and resuspended in BSS to achieve a final cell density of 25,000/0.1 ml for intravitreal injection.
Twenty-four hours after injection of the fibroblasts, one group of rabbits receives an intravitreal injection of 25 ug 1B4-RA in 0.1 ml PBS. The other group receives an intravitreal injection of 0.1 ml PBS. Eyes are examined by indirect ophthalmoscopy on days 1, 3, 7, 14, and 28 after injection. Appearance of the retina and fundus are documented by fundus photography. The toxic changes observed are graded in five stages according to their ophthalmoscopic appearance: stage 0= clear fundus; stage 1 = gray retinal area near the optic disc; stage 2= retina attached, but decreased vascularity and small rays; stage 3= retinal detachment without tear; stage 4=retinal detachment with tear. At the end of the study, the animals are sacrificed and the eyes enucleated. Portions of the posterior fundus are fixed, processed and embedded for histological examination. Activity of the immunotoxin is determined by comparing the results from the control and the treated groups. Example 10 Conjugation of Daunomycin to Carrier Proteins: Preparation of DM-BSA-DPDPB Forty mg of l-ethyl-3-dimethylaminopropylcarbodiimide (EDC) (Pierce) and 80 mg N-hydroxysulfosuccinimide (sulfo-NHS) (Pierce) were added to 1 ml of bovine serum albumin (BSA) (Sigma) dissolved in 2-(N-Morpholino)ethanesulfonic acid buffer (MES), (10 mg/ml in 50 mM MES pH 6.0) and incubated for one minute. Five mg of daunomycin in 0.2 ml of water was added to this solution followed by incubation for 2 hours at room temperature and overnight at 4°C. The reaction mixture was centrifuged (3000 x g, 30 minutes) to remove any precipitation formed during the reaction and the supernatant containing the BSA- DM conjugate was desalted over a Econo-PaclODG column (Bio Rad) (10 ml bed volume) equilibrated with 50 mM Tris buffer pH 8.0. Dithiothreitol (DTT) was added to give a final concentration of 50 mM followed by incubation at room temperature for 50 minutes. The reduced BSA-DM was desalted over a 1.5 x 32 cm Sephadex G-25 column equilibrated with PBS containing 1 mM EDTA. One 4-di-3'-(2'pyridyldithio)-propionamido) butane (DPDPB) (Pierce) (0.1 ml at 10 mg/ml in DMSO) was added per ml of BSA-DM and allowed to react for 45 minutes at room temperature. Following incubation, unconjugated reactants were removed by desalting over a 1.5 x 32 cm Sephadex G-25 column equilibrated with PBS containing 1 mM EDTA. BSA-DM-DPDPB conjugate was used within one hour for coupling to ECM binding proteins as described below.
Example U Conjugation of DM-BSA-DPDPB to 7G12 Fab'
Purified 7G12 IgG (2.3 mg/ml, 39 ml) in 0.1 M citrate buffer pH 3.8 was mixed with pepsin (Sigma, 3700U/mg, 0.9 ml of a 1 mg/ml solution) and incubated for four hours at 37°C. The reaction was stopped by adjusting the pH to 7.0 with 3.0 ml of 3M Tris buffer pH 8.0. DTT was added to give a final concentration of 50 mM followed by incubation at 37 °C for 30 minutes. The reduced 7G12 Fab' was isolated by desalting over a 1.5 x 32 cm Sephadex G-25 column equilibrated with PBS containing 1 mM EDTA. DM-BSA-DPDPB (17 ml containing 0.13 mg daunomycin/ml) was mixed with 30 ml of reduced 7G12 Fab' (1.4 mg/ml) and incubated for one hour at room temperature followed by 48 hours at 4°C. Following incubation, the Fab '-BSA- DM conjugate was centrifuged to remove insoluble precipitates. The supernatant was passed through a 0.45 micron filter and applied to a anion exchange column (HITrap Q, 2 ml, Pharmacia) equilibrated with PBS. Bound conjugate was eluted with PBS containing 2 M NaCl. Fractions containing 7G12 Fab'-BSA-DM were pooled and applied to a 1.5 x 28 cm Sephadex G-25 column equilibrated with PBS. Fractions containing protein and daunomycin eluting in the column void volume were pooled and stored at -20°C.
Example 12
Conjugation of DM-BSA-DPDPB to the Collagen Binding Domain of Fibronectin (FnCD) Purified bovine plasma fibronectin (1.0 mg/ml) in 50 mM Tris buffer pH 8.9 was digested with thermolysin (Sigma, 5 μ\ of a 1 mg/ml stock solution per ml of fibronectin) dissolved in 50 mM Tris buffer pH 8.0 for 4 hours at 37°C. The reaction was stopped by the addition of EDTA to give a final concentration of 50 mM. The digested fibronectin was applied to a 27 ml gelatin sepharose (Pharmacia) column and the column was washed with 50 mM Tris buffer pH 8.0 containing 10 mM EDTA. Collagen binding fragments were eluted from the column with 4 M urea in 50 mM Tris buffer, pH 8.0 and fractions containing proteins as determined by absorbance at 280 nm were pooled. Collagen binding fragments containing the fibronectin collagen binding (FnCD) domain were desalted into 50 mM Tris pH 8.0 using a 5 x 15 cm Sephadex G-25 column and pooled. FnCD was then applied to a 2 ml HITrap Q column equilibrated with the same buffer. FnCD fractions were eluted with 50 mM Tris pH 8.0 containing 1 M NaCL, pooled and desalted into 0.2 M sodium phosphate buffer pH 8.0 containing 1 mM EDTA using a 1.5 x 28 cm Sephadex G-25 column. Purified FnCD was treated with a fivefold molar excess of 2-imminothiolane-HCl (2-IT) (Pierce) and incubated for 60 minutes at 30CC. Following incubation, the derivatized FnCD was desalted into PBS using 1.5 x32 cm Sephadex G-25 column equilibrated with PBS containing 1 mM EDTA. FnCD-2IT (15 ml, 4.86 mg/ml) was mixed with DM-BSA-DPDPB (22.4 ml, 0.28 mg/ml) prepared as described above. The mixture was incubated 2 hours at room temperature and 48 hours at 4°C. FnCD-BSA-DM was purified by passage over an anion exchange chromatography (HITrap Q, 5 ml column) equilibrated with PBS. The FnCD- BSA-DM conjugate was eluted from the column using 2 M NaCl in PBS. Fractions containing protein and daunomycin were pooled and desalted over a 1.5 x 28 cm Sephadex G-25 column and stored at -20°C.
Example 13 Conjugation of DM-BSA-DPDPB to
Hyaluronic Acid Binding Peptides A peptide binding hyaluronic acid (Goetinck, et al, (1987) J. Cell Bio. 105:2403-2408) containing a cysetine at the amino terminal end was synthesized. The peptide sequence is shown below. H-Cys-Ala-Gly-Txp-Leu-Ser-Asp-Gly-Ser-Val-Gn-Tyr-Pro-Ile-Thr-
Lys-Pro-Arg-Glu-Pro-OH
Synthesis was performed on an Applied Biosystems Peptide Synthesizer Model 431 A. The peptide was synthesized using standard solid peptide synthesis employing Fmoc chemistry. The side chain protecting groups for the amino acids were: t-butyl for Asp, Glu, Ser, Thr, Tyr; trityl for Ala, Cys, Gin, His; Pmo for Arg; and Boc for Lys. Fmoc amino acids were activated using one equivalent of 0.45M 2-(lH-benzotriazol-l-yl)l,l,3,3-tetraethyl-uronium hexaflurophoshate (HBTU)/ 1 hydroxybenzotriazole (HOBt) solution and two equivalents of N,N- diisopropylethylamine (DIEA). Synthesis was started with 2-chlorotrityl resin. Fmoc protecting groups were removed using 25% piperidine/NMP.
Deprotection and cleavage of the peptide from the resin was performed using trifluroacetic acid (TFA) in the presence of a scavenger mixture (0.75 g phenol, 0.25 ml EDT, 0.5 ml thioanisole, 0.5 ml water and 10 ml TFA) for three hours at room temperature. The peptide was purified to greater than 95% purity by reverse phase HPLC using a C18 column with an AB gradient from 0% B where A is 0.1 % TFA in water and B is 0.08% TFA in acetonitrile.
Seventeen mg of lyophilized peptide was resuspended in 1 ml of water and added to 20.0 ml of BSA-DM-DPDPB in PBS containing 1 mM EDTA followed by overnight incubation at 4°C. The conjugate was purified by chromatography over a 1 ml HITrap Q column equilibrated with PBS. The conjugate was eluted from the column using 2 M NaCl in PBS and fractions containing protein and daunomycin were pooled and applied to a 1.5 x 28 cm Sephadex G-25 column equilibrated with PBS. The peptide-BSA-DM conjugate fraction eluting in the column void volume was collected and stored at -20°C.
Example 14 Conjugation of Daunomycin to Hyaluronic Acid Binding Peptides Containing a Terminal Hydrazide Group
A peptide binding hyaluronic acid was synthesized using standard Fmoc solid phase synthesis chemistry starting with Wang resin and using appropriate N-Fmoc-amino acids. Side chain protecting groups were tert-Butyl ethers and esters for Asp, Ser and Glu; Boc for Lys; and trityl group for Asn. Fmoc removal was performed with piperidine-DMF, followed by washing with DMF. Coupling was achieved by combining solid Fmoc-amino acids, HoBt and benzotriazol-1-yl-oly tris (dimethylamino) phosphonium hexafluorophosphate (BOP) and n-methylmorpholine (NMM) under nitrogen at 25 °C. Following the addition of the last amino acid residue and deblocking of the terminal NH2 group, the peptide was treated with an excess of succinic anhydride. Following washing, an excess of tert-butyl carbazate (Aldrich) was added and coupled to the terminal carboxyl group using carbodiimide. Protected peptide resin was then dried in vacuum and cleaved with TFA-scavenger mix solvent. After four hours at 25 °C, the cleavage mixture was filtered, precipitated with ether, collected by centrifugation and lyophilized. The peptide sequence with the terminal hydrazide group is shown below.
NH2-NH-C(O)-CH2-CH2-C(O)-NH-Leu-Ala-Leu-Asp-Trp-Cys-Asn- Ala-Gly-Trp-Leu-Ser-Asp-Gly-Ser-Val-Gn-Tyr-Pro-Ile-Thr-Lys-Pro- Arg-Glu-Pro-Cys-Gly-NH2 Thirty mg of lyophilized peptide was resuspended in 2.0 ml 0.2 M sodium acetate buffer pH 6.0 and added to 0.43 ml of daunomycin (28 mg/ml) followed by overnight incubation at 4°C. DTT was added to a final concentration of 50 mM followed by incubation at room temperature for 2 hours. The conjugate was centrifuged, passed through a 0.45 micron filter and purified by chromatography on a 1.5 x 15 cm Sephadex G-10 column equilibrated with PBS. The peptide-Hz-DM conjugate fraction eluted in the column void volume while free daunomycin came off the column considerably later.
Example 15 Conjugation of Daunomycin to BSA-Hvdrazide a. Preparation of BSA-Hz Coupled to Daunomycin.
BSA containing reactive hydrazide groups (BSA-Hz) was prepared as follows. To 250 mg BSA in 34.4 ml of water was added 3 g adipic acid dihydrazide (Sigma) with stirring. The pH was adjusted to 4.75 with 1 N HCl and 90 mg EDC were added. The mixture was allowed to react for one hour at room temperature with stirring while maintaining pH at 4.75. Following reaction, the solution was dialyzed at 4°C against distilled water to remove unreacted dihydrazide and EDC and stored at 4°C.
BSA-Hz (0.5 ml, 3.8 mg/ml in 0.2 M sodium acetate buffer pH 6.0) was mixed with 0.187 mg daunomycin in 7.5 μl of water. The mixture was incubated overnight at room temperature followed by chromatography over a Econo PaclODG column equilibrated with 0.2 M sodium acetate pH 6.0. The BSA-Hz- DM conjugate fraction eluted in the column void volume while free daunomycin came off the column considerably later. The concentration of daunomycin in the conjugate fraction was determined spectrophotometrically. The molecular substitution ratio was 12 molecules of daunomycin per molecule of BSA. b. Preparation of Peptides Derived from BSA-Hz Coupled to Daunomycin. Three ml of BSA-Hz (3.9 mg/ml in 0.1 M sodium citrate buffer pH 3.8) was mixed with pepsin (Sigma) (1.0 mg/ml stock in citrate buffer) to give a 2% (w/w) final pepsin concentration. The mixture was incubated overnight at 37°C and the reaction stopped by the addition of 0.75 ml 1 M Tris buffer pH 8.0. Two ml of this preparation was desalted over a 1.5 x 15 cm Sephadex G-25 column equilibrated with 0.1 M sodium acetate buffer pH 6.0. To 2.5 ml of the desalted BSA peptide-Hz mixture was added 3 mg daunomycin in 0.12 ml water followed by incubation at room temperature overnight. BSA peptide-Hz-DM was separated from free DM by chromatography over a 1.5 x 15 cm Sephadex G-10 column equilibrated with PBS. The BSA peptide-Hz-DM conjugate fraction eluted in the column void volume while free daunomycin came off the column considerably later.
Example 16
Conjugation of 7G12 to BSA Peptide-Hz-DM Purified 7G12 IgG in PBS (5 ml at 2.0 mg/ml) is treated with a fivefold molar excess of N-succinimidyl-32-pyridyldithiopropionate (SPDP) (1 mg/ml stock solution in absolute ethanol) with rapid mixing. Following incubation for 30 minutes at room temperature, the derivatized IgG is separated from unreacted
SPDP by chromatography over a 1.5 x 40 cm Sephadex G-25 column equilibrated with PBS. Fractions containing protein as determined by absorbance at 280 nm are pooled and stored at 4°C. BSA peptide Hz-DM (2 mg protein/ml. 5 ml) in PBS is treated with DTT to give a final 50 mM concentration followed by incubation at room temperature for 30 minutes. The reduced conjugate is separated from DTT by passage over a 1.5 x 50 cm Sephadex G-10 column. The conjugate is eluted from the column in the void volume and the protein and daunomycin containing fractions are pooled. Five ml of reduced BSA peptide-Hz- DM conjugate is mixed with 5 ml of SPDP derivatized IgG and incubated at room temperature for one hour followed by incubation at 4°C overnight. The
7G12-BSA peptide -DM conjugate is then purified by chromatography over a 1.5 x 120 cm Sephadex G-50 column equilibrated with PBS. Fractions eluting in the column void column containing protein and daunomycin are collected, pooled and stored at -20°C.
It is evident from the above results, that modulation of wound healing can be obtained using the conjugates of the subject invention. It is found that one can use conjugates of antibodies that target particular components of the extracellular matrix of the sclera and/or the conjunctiva, or of the vitreous coupled to an antiproliferative agent to inhibit proliferation of cells into a surgical wound site such as is introduced during glaucoma filtering surgery or as a result of trauma to the eye as is observed in PVR. All publications and patent applications mentioned in the specification are indicative of the level of skill of those skilled in the art to which this invention pertains. All publications and patent applications are herein incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference.
Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, it would be obvio us that certain changes and modifications may be practiced within the scope of independent claims.
What is claimed is:
1. A method for modulating wound healing in a tissue of a mammalian host, said method comprising: contacting said tissue with a conjugate comprising a cell proliferation modulating agent and a first member of a specific binding pair, wherein said first member binds with high affinity to a second member of said specific binding pair, and wherein said second member is a binding site for said first member on a component of an extracellular matrix in said tissue.
2. The method according to Claim 1, wherein said component is a collagen, a proteoglycan or a fibronectin.
3. The method according to Claim 2, wherein said tissue is sclera or conjunctiva.
4. The method according to Claim 3, wherein said tissue is an epiretinal membrane.
5. The method according to Claim 1, wherein said modulating is inhibiting.
6. The method according to Claim 5, wherein said cell proliferation modulating agent is an antimetabolite or an antimitotic.
7. The method according to Claim 6, wherein said antimitotic is daunomycin.
8. The method according to Claim 5, wherein said cell proliferation modulating agent is a toxin or a toxic portion of a toxin.
9. The method according to Claim 8, wherein said toxin is ricin.

Claims

10. The method according to Claim 1 , wherein said first member is an antibody.
11. The method according to Claim 10, wherein said antibody is a monoclonal antibody.
12. The method according to Claim 10, wherein said antibody is a bifunctional antibody.
13. The method according to Claim 11, wherein said first member comprises a binding domain of a polypeptide.
14. The method according to Claim 13, wherein said component is a collagen.
15. The method according to Claim 14, wherein said polypeptide is fibronectin.
16. The method according to Claim 1, wherein said cell proliferation modulating agent and said first member are conjoined by an acid labile linking group, a linking group which is cleaved by an enzyme endogenous to said tissue, or a group which is reduced by a reducing agent.
17. The method according to Claim 1, wherein said conjugate further comprises a protein carrier interposed between said cell proliferation modulating agent and said first member.
18. The method according to Claim 17, wherein said protein carrier is serum albumin.
19. The method according to Claim 18, wherein said cell proliferation modulating agent comprises 10 to 30 molecules of agent per molecule of said carrier protein. 20. The method according to Claim 1, wherein said wound is a fistula.
21. The method according to Claim 1, wherein said component is hyaluronic acid. 22. The method according to Claim 21, wherein said first member is hyaluronan binding protein.
23. The method according to Claim 22, wherein said hyaluronan binding protein is a link protein.
24. A method for inhibiting healing of a fistula, said method comprising: contacting tissue abutting said fistula with a conjugate comprising a cytotoxic agent and a first member of a specific binding pair which binds with high affinity to a second member of said specific binding pair, wherein said first member comprises an antibody or a macromolecule comprising a binding domain of a polypeptide, and wherein said second member is a binding site for said first member on a component of an extracellular matrix in said tissue.
25. The method according to Claim 25, wherein said tissue is sclera, conjunctiva, or an epiretinal membrane.
26. The method according to Claim 25, wherein said component is a collagen.
27. The method according to Claim 25, wherein said polypeptide is a fibronectin.
28. The method according to Claim 25, wherein said first member is an antibody.
29. The method according to Claim 25, wherein said cytotoxic agent is an antimetabolite or an antimitotic. 30. The method according to Claim 30, wherein said antimitotic is daunomycin.
31. The method according to Claim 25, wherein said cytotoxic agent is a ricin A chain.
32. The method according to Claim 25, wherein said conjugate is in a physiologically acceptable carrier.
33. A method for inhibiting growth of cells migrating into the extracellular matrix of tissue abutting a fistula in ocular tissue, said method comprising: contacting said tissue with a conjugate comprising an antiproliferative agent and a macromolecule which binds with high affinity to a component of said extracellular matrix.
34. The method according to Claim 33, wherein said antiproliferative agent is daunomycin.
35. The method according to Claim 33, wherein said component is a collagen, a fibronectin, or a proteoglycan.
36. The method according to Claim 33, wherein said macromolecule comprises an antibody or a fragment thereof.
37. The method according to Claim 33, wherein said macromolecule comprises a collagen-binding domain obtainable from a fibronectin.
38. A method for maintaining a fistula in ocular tissue in a mammal, said method comprising: creating a fistula site in said ocular tissue; and contacting tissue abutting said fistula site with a conjugate comprising daunomycin and a macromolecule which comprises a domain which binds with high affinity t a component of an extracellular matrix of said tissue.
39. The method according to Claim 38, wherein said component is a collagen, a fibronectin, or a proteoglycan
40. The method according to Claim 38, wherein said macromolecule comprises an antibody or a collagen-binding domain obtainable from fibronectin.
41. A composition comprising: a conjugate comprising a cytotoxic agent and a macromolecule which comprises a first domain which binds with high affinity to a component of an extracellular matrix in ocular tissue.
42. The method according to Claim 41, wherein said ocular tissue is sclera, conjunctiva or an epiretinal membrane.
43. The composition according to Claim 42, wherein said cytotoxic agent is daunomycin.
44. The composition according to Claim 42, wherein said cytotoxic agent comprises Ricin A chain.
45. The composition according to Claim 41, wherein said component is a collagen, a proteoglycan or a fibronectin
46. The composition according to Claim 41, wherein said macromolecule further comprises a second domain which binds with high affinity to a cell in said ocular tissue.
47. The composition according to Claim 46, wherein said cell is a fibroblast. 48. The composition according to Claim 47, wherein said cy toxic agent comprises ricin A chain toxin.
49. The composition according to Claim 47, wherein said macromolecule is an antibody.
50. A composition comprising: a conjugate comprising daunomycin and a first macromolecule which comprises a domain which binds with high affinity to a collagen, a fibronectin, or a proteoglycan.
51. The composition according to Claim 50, wherein said first macromolecule comprises an antibody, a fragment of an antibody, or a binding domain obtainable from a polypeptide.
52. The composition according to Claim 51, wherein said antibody is a monoclonal antibody.
53. The composition according to Claim 51, wherein said polypeptide is fibronectin.
54. The composition according to Claim 50, wherein said conjugate further comprises a carrier molecule comprising a second macromolecule between said daunomycin and said first macromolecule.
55. The composition according to Claim 54, wherein said second macromolecule comprises human serum albumin.
56. The composition according to Claim 50, wherein said conjugate is reversibly bound to a solid support.
57. A composition comprising: a conjugate comprising cytotoxic agent and a bifunctional antibody which comprises a first domain which binds with high affinity to a collagen and a second domain which binds with high affinity to a fibroblast.
58. The composition according to Claim 57, wherein said cytotoxic agent is daunomycin.
59. The composition according to Claim 58, wherein said conjugate further comprises human serum albumin as a carrier molecule between said daunomycin and said bifunctional antibody.
60. The composition according to Claim 57, wherein said cytotoxic agent comprises ricin A chain toxin.
61. The composition according to Claim 58 or 60, wherein said bifunctional antibody is a bifunctional monoclonal antibody.
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