WO1995027517A1 - Pharmaceutical agent for local application and a method of obtaining same - Google Patents

Abstract

The proposed haemostatic and bactericidal pharmaceutical agent for local application consists of gelatin, furacilin, gentamycin sulphate, calcium chloride, sulphonol and water in the following proportions (wt %): gelatin 60-80; furacilin 0.1-0.4; calcium chloride 0.1-0.4; gentamycin sulphate 10.0-30.0; sulphonol 0.7-3.0; water the balance. The method of obtaining the proposed pharmaceutical substance involves mixing a solution of gelatin in an aqueous solution of furacilin with an aqueous solution of calcium chloride, formaldehyde and sulphonol, and making the mixture froth until a volume of foam at least twice that of the original mixture is obtained. An aqueous solution of gentamycin sulphate is then added to the foam as the frothing process continues. The resulting foam is frozen at a temperature of -50 °C or below to form a crystalline structure. The resulting product is dried by sublimation at an initial temperature of between -50 and -30 °C and a final temperature of 38-40 °C before being sterilized by irradiation.

Description

95/27517 ΡSΤLSH94ΛYU073 ϊaChZBΗY PΡΕPΑΡΑΤ AJ LΟΚΑLΗΟGΟ PΡIΜYΗYAΗIYA and SPΟSΟB _ZG0 PΟLUCHΕΗZH Οblasτ τeχniκi Ηasτοyaschee izοbρeτenie οτnοsiτsya κ οblasτi copper- 5 Ch'ing and imennο κ medical πρeπaρaτam gemοsτaτichesκοgο and anτibaκτeρialnοgο deysτviya for lοκalnοgο πρimene- Nia and sποsοbam iχ ποlucheniya.

Pροblema προφilaκτiκi and treatment ρanevοy inφeκtsii vο vseχ οblasτyaχ χiρuρgii yavlyaeτsya very aκτualnοy τesnο and 10 associated with neοbχοdimοsτyu οbesπecheniya bysτροgο and sτοyκοgο gemοsτaza, τem bοlee, chτο neποlny gemοsτaz mοzheτ yaviτsya dοποlniτelnοy πρichinοy seπτichesκiχ οs- lοzhneny. In addition to the parenteral methods of administering antibacterial drugs, the importance of 15 has a significant value for the creation of a high rate of growth in them. Β The connection with this portable device is the use of a drug with a long-term active drug. Pρedshesτvuyuschy uροven τeχniκi 20 Izvesτen gemοsτaτichesκy πρeπaρaτ for lοκalnοgο πρimeneniya / £ [7 83093 W / κοτορy πρedsτavlyaeτ sοbοy gubκu in sοsτav κοτοροy vχοdiτ πlazma κροvi chelοveκa τροmbοπlasτin and / τκan mοzga chelοveκa /. This product is equipped with components that are identical to those used in its manufacture and that it is not suitable for the manufacture of raw materials. Otherwise, the use of the product is very troublesome, as it is very difficult to use and could result in a loss of life. There is a curative preparation for local use, which is in possession. hemostatic and antibacterial action / ϋ _in Μ; _ 1E2397 /. The following components are included in the composition of the product: gelatin, furacilin, Calcium coolant-35 reed, sulfonyl, water. The preparation is prepared in the following way: gelatine is dissolved in water at a temperature of 40-50 ° C when mixed; in addition add furatsilin, water \ УΟ 95/27517 ΡСΤ / ΚΙΙ9

- 2 - derivatives of maldehyde, water :; sulphate and calcium δ chloride; The resulting mixture is excreted at a temperature of 30-50 ° C for 30 minutes. ; After increasing the volume of 5 foams, it is poured into the form and dried for 48-60 hours. at a room temperature; The obtained product is sterilized at a temperature of 140 ° С.

The product received in the form of a preparation in the form of a sponge possesses a small hemostatic activity, since there is an inadequate addiction to 10 This is explained by the fact that in the process of irradiating, the foam is emitted from the air, which is not emitted, that is not emitted, The amount of moisture in the appliance, in such a large amount, significantly reduces the discharge of its storage. Otherwise, this product does not possess the long-acting antibacterial action.

Ρasκρyτie izοbρeτeniya 20 Β οsnοvu izοbρeτeniya ποlοzhena sοzdaniya problem for left-chebnοgο πρeπaρaτa lοκalnοgο πρimeneniya, οbesπechiva- yuschegο bysτρy and sτοyκy gemοsτaτichesκy eφφeκτ in ορga- nism, οbladayuschegο προlοngiροvannyϊνϊ anτibaκτeρialnym deysτviem not vyzyvayuschegο ποbοchnyχ ρeaκtsy ορganiz- to 25 IU, and τaκzhe ρazρabοτκi sποsοba egο radiation.

Sοglasnο izοbρeτeniyu πρedlοzhen therapeutic πρeπaρaτ gemοsτaτichesκοgο and προlοngiροvannοgο anτibaκτeρialnοgο deysτviya for lοκalnοgο πρimeneniya, sοdeρ; _aschi; ϊ zhelaτin, φuρatsilin, κaltsy χlορid, and sulφοnοl vοdu, κοτορy sο- 30 deρzhiτ τaκzhe anτibiοτiκ - genτamitsin sulφaτ πρi following sοοτnοshenii κοmποnenτοv present in wt. : gelatin 60-80 furacilin 0.1-0.4 calcium Calcium 0.1-0.4

35 gentamycin sulfate 10-30 sulfon 0.7-3.0 other water.

The proposed appliance does not include sponge with ΡСΤЯШ94 / 00073

- 3 - a pronounced industrial structure with a bright yellow color. The high-speed industrial structure of the sponge is indicated by the system ensures the mechanism of the hemostatic effect of the drug 5 in the country. Hemostatic spongy is ensured by its adhesive properties, stimulation of the formation of the process and the activation of coagulation.

It is known that the main source of the filament is

10 of the current processes in our country are the rhizome, which may also have an inhibitory effect on the development of hemostatic disease. The sponge, thanks to the long-term antibacterial interactions, removes the indicated inhibitory effect and facilitates the treatment

15 Hemostatic disease.

As it was stated above, in the presence of the proposed product, gelatine enters. Gelatin is a basic white drug, which is necessary for the reception of the intended action. Other than that, it is known that gelatin

20 has a small back-up action and is able to enter into a void of contact with other substances. Favored quantity of gelatin is optimal. With a decrease in the amount of gelatin less than 60 wt. cannot receive the preparation in the form of a sponge with a pronounced

25 natural buildings. An increase in the amount of gelatin over 80 wt. % does not improve the quality of the product.

Pharmacilin as a part of the product offers the product of an antiseptic. Amount of 0.1-0.4 wt.% Is

30 is necessary and sufficient to ensure this property.

Calcium chloride is a means of repairing and accepting participation in the formation of hemostasis. With a decrease in the amount of calcium, less than less

35 0.1 wt. Δ not achievable hemostasis, and an increase in the amount of galactic acid above 0.4 wt. it does not increase hemostatic effect. Genetamycin has a wide range of antibiotic - 4 - sectoral action. With this antibiotic, sensible belypins of mixtures of Rana. A well-known quantity of anti-biotics is sufficient to ensure the antibiotic 5 drug's effect.

Sulphon is a traditional drug-active substance that prevents the drug / sponge /.

Sοglasnο izοbρeτeniyu, sποsοb ποlucheniya lechebnοgο 10 πρeπaρaτa gemοsτaτichesκοgο and προlοngiροvannοgο anτibaκ- τeρialnοgο deysτviya vκlyuchaeτ mixing ρasτvορa desired τina, vοdnyχ ρasτvοροv φuρatsilina, κaltsiya χlορida, φορmaldegida, sulφοnοla, vsπenivanie ποluchennοy sme- si sushκu and sτeρilizatsiyu in κοτοροm πρi mixing 15 used Uses a solution of directly heated gel in the fluorocarbon aperture; foaming of the mixture has a lower temperature of the mixture, which increases its alneyshegο vsπenivaniya in πenu vvοdyaτ vοdny ρasτvορ 20 genτamitsina sulφaτa and zaτem πeρed sushκοy ποluchennuyu πenu zamορazhivayuτ πρi τemπeρaτuρe not higher than minus 50 ° C for φορmiροvaniya κρisτallichesκοy sτρuκτuρy προduκτa, sushκu ποluchennοgο προduκτa οsuschesτvlyayuτ meτοdοm subli- mation πρi nachalnοy τemπeρaτuρe -50 - -30 ° C 25 and the final temperature of 38-40 ° C, and the sterilization is carried out by radiative irradiation, and at the same time, the increase in the mixture is a value of 0.5–0.5%:

30 Calcium Chloride 0.005-0.03 sulfon 0.04-0.25 gentamycin 0.6-2.5 φ malaldehyde 0.05-0.09 other water.

35 As stated above, in the proposed method, we use heated gelatin. The use of genuine gel is necessary for you to take advantage of the undesirable reactions in using the product and using it. \ νθ 95/27517 ΡСΤ / ΤШ94 / 00

- 5 - Prata.

Pρi isποlzοvanii ρasτvορa zhelaτina in φuρatsiline sοvmesτnο with dρugimi κοmποnenτami in chasτnοsτi with φορ- 5 maldegidοm in προtsesse vsπenivaniya sοzdayuτsya uslοviya for ποlucheniya πρeπaρaτa, οbladayuschegο προlοngiροvannym anτibaκτeρialnym deysτviem. This is due to the fact that partly free bonds of gelatine enter into a viable reversible interaction with antibiotics. Κροме τοгο,

If you use gelatin in faracilin combined with other components, you should expect that fucacilin will not be able to remove the usual commercially available product. However, it turned out that, for the sake of foaming a mixture of furacilin, it is also the same as antibiotic, and it is also absent

15 et with gelatin in a bond. This means that you have a stable, easy-to-use foam machine, which ensures that you don’t have any problems.

Primary foaming of the mixture

20 οbema πeny, πρevyshayuschegο πeρvοnachalny οbem mixture of not less than 2 ρaza, οbesπechivaeτ hereinafter ποlu- chenie πρeπaρaτa with dοsτaτοchnοy πορisτοsτyο, neοbχοdimοy for οbesπecheniya eφφeκτivnοgο gemοsτaτichesκοgο deιϊsτviya πρeπaρaτa.

25 Further foaming of the mixture continues with a simultaneous bloating of the antibiotic-genamicin sulfate. The presence of an antibiotic in a mixture containing gelatin, phosphor maldehyde and sulfonyl, is beneficial, as indicated above, for the formation of invalid bonds of this antibiotic

30 with gelatin to ensure the sustained action of sulfamycin sulfate in the drug. The final stage of increasing the volume of foam is shared by the variant of implementation of the technology of foaming. The greater the volume of the mixture, the larger the volume of the mixture.

35th year, the obtained product is better, which increases its hematostatic effect.

The subsequent stages of freezing the foamed foam and drying it by sublimation ensure the production of pry- \ UΟ 95/27517 _

- 6 - The machine is equipped with a stable appliance.

As stated above, the temperature of the freezing temperature is minus 50 ° С. With this temperature 5, the stabilization of native foam is already taking place. The lower the temperature, the greater the speed of freezing foam. The lower temperature freezing section of the foam is connected with the facilities used.

Sushκa προduκτa in vybρannοm ρezhime daeτ vοzmοzhnο- Yu sτ ποluchiτ πρeπaρaτ with sοχρaneniem πeρvοnachalnοy sτρu- κτuρy πeρy, aκτivnοsτi anτibiοτiκa and εgο προlοngiρuyuschiχ svοysτv, τaκ κaκ in eτiχ uslοviyaχ προisχοdiτ first sub- limatsiya zamοροzhεnnοy πεny and zaτεm moisture removal. With a decrease in temperature below minus 50 ° С, the drying time will increase

15 is readable, and if the temperature rises above 40 ° C, there may be a partial inactivation of the antibiotic.

If there is a loss of radiation, the product is protected, and there are no errors in it. 20

The best variant of the invention

The claims were examined in an experiment on animals and in a clinic on people. 25 The study of hemostatic properties of the device was carried out in experiments for 15 dogs / 60 studies /. In the meantime, in the middle of the accident, we had to re-operate the unit after opening the other brain. After that, we took into account the artefactual conditions.

? Lots of brain damage, and also the walls of the sagittal sinus, causing different intensity of arrhythmia and invasion.

At the same time, at most lively ships they were waiting for arterial and venous vessels of the cerebral hearing,

° χ by measuring the diameters of the eye The diameter of the approved vessels was adjusted from 0.02-0.6 mm to 1, 0-1, 2 mm.

Convenient and non-operating parts weighed and disposed of small pieces or small goods. \ νθ 95/27517 ΡСΤЛШ94 / 00073

- 7 -

The full rest area of the accessory was secured by the second. The results are reported in Table 1.

Table 1

Variety of property Proposed first-hand range of ready-to-use vessels, new arrivals, warm water

1.0 / οπρε- ct- / οπρ- ctase, deleza, dεlε- min. niϊ / min. niy /

0,0ρτερ- 0.02 • 5 strings 0.6 30 1-2 10 8-10

Erythrocytes and venous co-0.06 • 20 courts 1.2 15 2-2.5 10 12-15

The outer sagittal sinus and its 25 0.2-1 15 0.5-1 10 15-18

From the table you can see that the proposed product is equipped with expressive installation properties, such as venous, arterial and arterial connexion,

30 exercises with brain injuries. The process of experimentation was established on the basis of. the adhesion of the product to the brain tissue and its fractions ...

Studying the antibacterial activity of a patient and having a direct cranial brain injury was at the same time

35 per 100 rises per category. After an incidence in the left forearm, they were disrupted due to the use of external devices and in case of accident With a sharp shotgun, they applied a direct trauma

40 brains. Β The company was introduced a foreign body / piece of food /, \ νθ 95/27517

- 8 - 0.1 ml suspension of microcultures. For infection, Ran used a blue strain or a strain of a bad hematological disease. 5 Κulτuρu sinegnοynοy πalοchκi vvοdili in ρanu in dοze 100x10 "miκροbnyχ τel and sτaφilοκοκκa - 200x10 miκροbnyχ τel Οba shτamma chuvsτviτelny κ genτamitsinu sulφaτu ddya προφilaκτiκi inφeκtsiοnnyχ οslοzhneny You are a ποlnyali πeρvichnuyu χiρuρgichεsκuyu οbρabοτκu ρany in sροκi..

10 24, 48, 72 and 120 hours from the moment of the occurrence of τρ of the wave. They poured the burden into the country twice: after three hours after the application of the trauma and the information of the trash, then after the completion of the breach of the oven, the process was completed. Dose for a single laying in a nursing home

15 vila 12 mg / 12000 mcg gentamicin sulfate /.

2 Table 2 contains the results of the treatment of the patient in combination with the treatment of the other

On the 20th of the year, the 8th –0th Fifth Finger, in Table 3, was turned off.

Table 2

25

thirty

35

\ νθ 95/27517

- 9 -

Table 3

There are 20 lively experiences in all of these countries, which have been prevented from occurring, and that they have been affected by

20 cerebral infections have developed infectious complications. With the observation of up to 3 weeks, there were 12 live.

Β group of animals, at cows and papilla erythrocytes were infected with blue sticks / table 2 /, infectious services removed at a time

25 treatment in combination with 24 hours after the accident in 00 cases, after 48 and 72 hours in 80 cases, after 120 hours in 70% of cases.

With the recession into the country of a small cultural community

30 chεsκοgο sτaφilοκοκκa / τablitsa 3 / πρi προvzdεnii περvich- nο: ϊ χiρuρgichesκοy οbρabοτκi in sοchετanii with πρεπaρaτοm cheρεz 24, 48 and 72 hours from the application mοmεnτa τρavmy invariant φeκtsiοnnyε οslοzhneniya οτsuτsτzοzali at vseχ zhivοτ- nyχ 100 and 120 cheρεz chasοv - 80 lively.

35 The state of lively experiences at the end of the entire observation period was satisfactory, all live survivors. Healing of the skin of a π ρ π:::::::: tension. \ νθ 95/27517 ΡСΤ / ΙШ94 / 00073

- 10 - According to the most recent medical examination: in all online business trials, the following symptoms were detected: 5 ... there was an exceptionally pronounced carcinoma of a known ugly meningoencephalitis.

Β Cases of an effective .. ρ ο φ ила ила ζ ζ ζ ζ ζ ζ ζ ζ ζ π π π π π π .. .. .. .. .. .. .. .. .. Β .. .. .. .. .. .. ...

10 In the case of a hysterical study in the regions of the former autonomous area for 21-30 days, it was found out that there was a very bad condition.

Confirming that you have the option of using the 5th option of combining with the combination of a military unit. Medical treatment was also a result of bacteriological treatment of the patient, and there is a medical / metabolic disease.

20 Β κοnτροlnyχ seρiyaχ οπyτοv πρi inφitsiροvanii ρan miκροbnοy κulτuρο: ϊ sπnegnοynοy πalοchκi and sτaφilο- κοκκa at vseχ zhivοτnyχ of ρan sown sοοτzeτsτvuyuschii myκροb and πρi isποlzοvanii sinegnοynοιϊ πalοchκi in cases ρyade τaκzhe from liκvορy κροvy s, chτο szidετεlsτvοva-

25 l ο of the generalization of the foreign transaction.

After a quick insertion of the drug into the rapa after 3 hours after the release of the trauma, a blue-fingered stick or a hemolytic lesion emerged from all the races. The first mortgage paid to the Republic of Armenia did not only delay the development of

30 tiε of the inception in all animals, but also in the case of performance: ^" ; processing in 70-80 -b cases of antibiotic _*

In the event of an accident, you will be left with a good idea after the first. Waste treatment in combination with

With 35 free mortgages, a larger blast was formerly steel. Β slunae οbsemeneniya ρan gεmοliτychesκim sτa ^~ ylοκοκκοm any case οdnο.νϊ eτοτ .yaικροb bis ιΣany not vysezalsya. At all live groups at this stage

Table 4

8 9 10

Κ ο Κ ω κ ρ ο

Οн Ο οβ

§ cβ ω ΕΗ

Ρn Ο

\ УΟ 95/27517 ΡСΤ / ΙШ94 / 00

- 13 - The experience of the plant and the sowing of seeds of the world of mixtures is not found.

Β gρuππe zhivοτnyχ, y κοτορyχ ρany inφitsiροvali Cu 5 negnοinοϊ πalοchκοy, ποsle προvedeniya πeρvichnοy χiρuρgi- chesκοy οbρabοτκi with ποvτορnym vvedεniem πρεπaρaτa chερεz 120 chasοv with mοmenτa nanesεniya τρavmy, eτοτ vοzbudiτel vysεvalsya of liκvορa οdnοm in the case of 10, and the SRI in πρi vvedε- Ranu hematolytic failure and the liver was

10 Rilen in all animals.

The long-term antimicrobial activity of the preparation was studied in an experiment for 50 cases.

After anesthesia from the left frontal-dark region, they were discharged from the skin in the middle and received information.

1δ of the absenteeism in addition to a quick and easy way was opened in the middle of the brain. With a scalpel, a direct brain injury was applied and a single unit was placed, containing 12,000 mg of sulfate gene.

20 hours after 30 minutes On 1, 3, 6, 12 hours, 1, 2, 4, 7, 14, and 21 days, 5 lively animals were scored every time. Before live, we took away from the jugular vein, cerebrospinal fluid from the large cerebral cyst, the cerebral canal at the hearth and introduced.

25 The concentration of sulphamycin gentamycin in the fluids of the urop organism of the uterus, the body of tissue and the extracted method of diffusion in the agar-20 were divided.

Average data on the content of genes of genamicin

30 sulphates in the cerebral canal, in the blue liquor and in the blood are found in table δ. Composition of gentamicin sulfate in the preparation - in table 6.

From the data shown in table 5, it follows that after 3 hours from the moment of introduction of the drug into the end station

The dose of gentamicin sulfate in the brain was maximal and amounted to 29 µg / g. For 3 days, the concentration of antibiotic was quite high - 22 mkg / g. 14

Table

Table 6

Ingredient Gentamicin Content of the Research Sulfate in the Extracted Preparation / Drug /

30 minutes 5 10900 1 hour 5 9000

3 hours 5 9000

6 hours 5 6000

1 day 5 4000

2 days 5 2200

4 days δ 1800

7 days δ 1000 4 days εy δ ЗδΟ 95/27517 ΡСΤЛΕШ94 / 00073

- fifteen -

С In the following stages, there was a gradual decrease in the content of gentamycin sulfate, only seven days after the administration of the drug was still / 5 / Even after 14 days after the introduction of the drug in the brain, there was still a certain number of antibiotics / 4 mkg / g /.

That is related to the content of gentamycin sulfate in the cerebrospinal fluid, which rapidly increased by 10 and reached the highest value in the range of 10–12 hours / month.

The gentamicin sulphate had already stopped after 30 minutes after the administration of the drug. and consisted of this mixture was maximum and amounted to 12 µg / ml. 15 After 15 following observation periods, from 1 hour to 4 days, the content of hematamycin sulphate was gradually reduced, and after 7 days the antibiotics were not found in the blood.

From the data of table 6, it follows that, after 30 minutes. After the introduction of the drug, the quantity of genetamycin sulfate 20 in it decreased from 12000 to 10900 mcg. С The next step is to reduce the content of genetamycin sulfate, which is associated with the absorption of the drug and the gradual release of antibiotics. Ечение Within 7 days after the introduction of the drug, the content of gentamycin 25 of the sulfate remains high - up to 1000 mcg.

After 14 days, the antibiotic in the extracted preparation was still divided and was 350 micrograms, and after 21 days it was not detected in the drug.

Ρezulτaτy προvεdennyχ isslεdοvany ποκazali, chτο 30 to 14 days τεcheniε προisχοdiτ ποsτeπennοε selection gene τamitsina sulφaτa of πρeπaρaτa in οκρuzhayuschuyu sρedu ορga- nism, sοzdavaya anτibaκτeρialnyε κοntsεnτρatsii in mοzgο- vο: ϊ τκani, sπinnο-mοzgοvοιϊ zhidκοsτi and κροvi.

In general, the proposed product is available for purchase with 35 wired anti-shipments.

The ratio of the product was studied in an experiment at 30 cases / in two experiments /.

Β περvοy seρii οπyτοv in 10-τi inτaκτnyχ zhivοτny ^χ \ UΟ 95/27517

- 16 - the physiological reaction of the main brain to the local community was studied: .. an increase in the amount of 10,000 to 20,000 mg of genamycin sulfate. The product placed 5 on the irrelevant headquarters of the main brain at the area of 1, 5χ 5 cm in the left front-temperate temporal region. The electronic sales unit was effected only up to 14 days from the moment the tax was charged on the main brain. Recording of biologic potentials was made on 4 10 channel encephalogroup and 8 channel encephalograf. And one of the living animals of this series did not have noticeable any kind of clinical reactions.

Electricity research also didn’t reveal in any case the potential for biologic changes in the brain in response to the delay. By the time of observation, up to 14 days from the moment of imposing a charge on the brain, the bioelectrical activity of the main brain was absent from all the living.

In the second series of experts, they studied the brain logic 20, the brain reaction of the main brain.

Lively of this series of experiences / 20 circles / caused a healthy brain trauma: 8 live / frontal groups did not enter; 12 live animals / experimental group / in a cerebral-cutaneous plant were laid with 25 preparations, containing 15,000 mcg of hentamycin sulfate.

On the 3rd, 7th and 14th day after the application of live traffic, the main brain was doubled and they were subjected to a histological investigation. The preparations treated the hematoxysilin-ezinim at Can-Gizonu, and also issued the order of 30 days and the imprisonment of them.

On the 3rd day of the accident, the maximum amount of live weight loss in the territory of the Republic of Kazakhstan was reduced to a small number of cases: in some cases, there was little chance of it. The message was received. In the area of the automatic delay, the source was discovered by the brain, acquired by the public. In this case, the experimental group was not found in the case of the accumulation of gemmata in any case. By taking advantage of the tight fit of the gearbox, it was safe to take advantage of the built-in safety gear. \ УΟ 95/27517 ΡСΤ / ΙШ94 / 00073

- 17 - τaz. The word was verbal.

In fact, in the case of livelihoods in animals in the cerebral arteries in the brain, a pathogen was detected, and there were δ However, these steps were followed by the identification of the limitations of the τ auto center in the form of φο-. peace in a new zone of newly formed vessels. On the seventh day from the time of application, the live and 10-month group experienced a slower or less frequent loss of health, which was less

In lively experimental groups, there was a clear identification of the development of ka-lagen ^ newworld. 15 In the course of the fourteenth days after applying the trauma, all living on the part of the brain showed an oversized glio-mesenchymal prong. Particularly revealed new vessels were revealed. Of changes. Okrasasy Nisslevskogo substance in the news did not notice. 20 Evaluation of the results of the case studies indicates that the process of repairing the cost of the average profit is low

Εdinsτvennοε ρazlichie zaκlyuchalοs in τοm, chτο in οπy- τaχ without πρimenεniya πρεπaρaτa in mοzgοvοm veschesτve in οblas- 2δ τi ρany vyyavlyalοs sκοπlenie κροvi with φορmiροvaniem hematoma τοm, τοgda κaκ πρi isποlzοvanii πρeπaρaτa any case οdnοm uκazannye phenomena were not observed.

Findings, results of investigations prove that there is no accident and impairment of 30 accidents

The claimed preparation was used in the clinic of the clinic and various individual medical conditions. Clinical studies were carried out for 2813 patients. The method of use of the device and its use depends on the purpose and area of its application. All cases to achieve a hemostatic effect of the drug on a vacant place for 1-2 minutes. .If the flow does not stop, then try - 18 - Again, they are laying down, so that they will not be able to reach the full hemostasis. In order to prevent the occurrence of infectious complications, the patient is to be reminded of any request to stop any injuries. It is immediately absorbed within 14 days, and therefore it can be sewn up in a wound.

For lechεniya gnοynyχ προtsessοv πρεπaρaτ zaκladyvayuτ in ρanu and ποslεduyuschiε περevyazκi προizvοdyaτ with ucheτοm bysτ- 10 ροτy egο ρassasyvaniya, κοτορaya naχοdiτsya πρyamοy in dependence simοsτi οτ κοlichesτva and χaρaκτερa gnοynοgο οτdεlyaεmοgο.

The preparation was also used for biological treatment of obstructed burns / burns, acute ulcers, acute ulcers /.

1 The total cure was cured by a large number of sick people in most of the cities, who received better food for one month, and two months and two months of fire.

The medicine used for the treatment and treatment of the neuropsychiatric process is controlled by the endocrine

20 to it of sulfamycin and depends on the size, distribution of the localization and localization of the process.

The total content of sulphate genetamycin and the use of the drug should not exceed 2 g when using the drug

2. Large quantities of the drug with sulfate gentamycin should not increase 2δ0 mg.

Non-hazardous products, which are suitable for biological treatment, are divided by the size of the disposed devices.

30 The results of the treatment are more painful with the use of the device in Table 7.

Table 7

Α1 ^; '. ^g Οblasτ πρimeneniya Κοl-vο Pοlοzhiτel- Zδ ^ππ bοlnyχ nyιϊ ρεzul- τaτ ^'absο-' ~~ lyuτnοε% chislο τ Τ Τ

1. Gnyny r any different προ- 480 470 ο 8 - 19 -

See table 7 below.

1 2 3 4 δ sources / abscesses, phlegms, 5 masses, pa- rapactocts, suppressed pans, /

2 Αππεіnditsitsі / phlegmonous., Gangenous, complicated para-apa-pendicular abscess and

Junior date / 29δ 288 98

3 Simple and erythrocytephritis, complicated by a transvesicular abscess, local ophthalmitis, an infamous erythrocytes, and a mechanical

15 yellow 32δ 312 96

4 Operation and maintenance of the military vehicle. Foreign access to Everting the territory of the western part of the territory of 20 spaces in the buried territory of the land and the inability to reduce it

6 Burning bodies of various bodies of various depths, areas and heaviness

7 Orthopedic blockade, 2δ syndrome of weakness of the sinus rheumatism. Electrical implantation surgery

8 Operations at the end of the year

9 Τροφichεсκиε ulcers 30 0

11 Automatic disruption of the stomach, tears, kidney, urinary bladder, pancreas

12 Concealed black and cerebral tavma Zδ. inconsequential and inconsequential

\ νθ 95/27517

- 20 -

Table 7

1 2 3 4 5

13 Closed black brain wave 5 with removal of the internal gamma 160 160 100

14 Most popular brain / bones, occlusion hydraulics / 31 δ 31 δ 100

10 1 δ Cases of malformations / arrhythmias and arterial aneurysms / 16 Purulent processes in non-obstructive patients, out of them: 1 δ are incurable

Brain process

Significance ρan

Егegοο:

Β group sick with ugly ryany in 98 cases

20, a clinical effect was observed: on the 2nd - 3rd day, the wounds began to be cleaned from a large section of food and on the 4th - 6th day were filled with healthy items. When the bacterium was inoculated in the treatment process, the sowing of the microculture disappeared immediately: golden and

2δ epidermal system, incidence, small intestine, blue fingertip in the multiplicity and in various associations. The treatment methods using the medicine, compared with other methods of treatment, were reduced to 1, 5 times and complete healing of the medicine was noted

30 hours from 28-30 days since the beginning of treatment. In patients with ^χ , a quick preparation was also taken with the aim of stopping the incidence, a steady hemostasis was reached within 40 seconds - 2 minutes.

When an appendix is issued, a complicated appendix

35 in 288 out of 295 patients, along with other ΡСΤ / ΚГ94 / 00073

By treatment methods, it would be possible to cleanse the patient after the treatment, remove the nest and heal the wound.

In these sickly ones, it was often sown. 5-year-old, sensitive to gentamycin. The case of Β ρyadε chaεv πρi presence φlegmοnοznyχ and gangρenοznyχ aππenditsiτο πρeπaρaτ πρεπyaτsτvοval vοzniκnοveniyu ποslεοπερatsiοnnyχ gnοιϊnyχ οslοzhnεny. In 2/3 of the patients with postoperative wounds, they were healed by primary intention.

10 Pοlοzhiτεlnyε ρezulτaτy were identified τaκzhe πρi isποlzοvanii πρεπaρaτa vο vρεmya προvedeniya οπeρatsii χοletsisτeκτοmii at bοlnyχ with οsτρym and χροnichesκim χοle- tsisτiτοm κaκ with tsεlyu προφilaκτiκi οslοzhnεny, and τaκ πρi presence τaκiχ ρazvivshiχsya οslοzhneny, κaκ abstsεssy,

15 local territories and others. 96 patients got better: they had a disinfectant at a time without complications from the hospital.

Use of an industrial appliance and obstructive injuries to an industrial appliance and

20 and the investigation, including vile complications, including varieties of various diseases, were also quite effective. After treatment, there is fresh water in the middle of the welfare / 30 more / and good health and / or long-term use of the room /

25 no suppurations were observed.

In the case of chronic diseases, they were complicated by accidents and injuries / 130 injured /, as well as by the apartment /

30 kokkom, a small finger or other associations and was sensitive to genamicin sulfate. The use of the product by administering the operation of the sequential system, in parallel with the parenteral administration of antibiotics, in 93 'all the results were obtained:

35 140 large, open pockets were sutured, at 118 they were liquefied under a specific tension, at 10 - torn. Only in 12 cases the treatment was unsuccessful. At 32 patients in the hospital, the names of the products were prepared \ νθ 95/27517 ΡСΤ / ΤШ94 / 000 3

- 22 - to the outpatient autoplasty.

Use of the drug and surgical interventions for the use of appendicides, cholecystitis, and carcinoma

A 5-motor device provided all cases of hemostatic effect within 1-2 minutes.

Οdnu of naibοleε τyazhelyχ gρuππ bοlκyχ, naχοdyaschiχ- Xia ποd observation sοsτavlyalο 58 chεlοvεκ with evεnτρatsi- 8ϊϊ ποlyχ ορganοv bρyushnοιϊ ποlοsτi in gnοynuyu ρanu πρi nε- 10 vοzmοzhnοsτi εε suturing and the presence of fistulae ποlyχ ορganοv, κοτορye chasτο οslοzhnyalis ποsleοπeρatsiοnnym πeρiτο- niτοm . It is usually the case from a factory, enteritis, enteric sticks, enterobacteria, asthma, which are differentiated by different assumptions. Named πρεπаρааа in

15, biologically beneficial treatment, along with the general methods of treatment, also involving abstraction, is beneficial for the well-being of 64%

20 On a biological basis, the treatment of the drug was also used in the integrated treatment of 282 patients with burns of heat. The results of treatment depended on the depth, area of the burn and the time of the start of treatment from the moment of the accident, which was caused by

On 25th, in turn, the severity of the disease. In 130 patients with 1-SH burns, a complete epithelization occurred in a delay of 7 to 30 days.

The largest patients with the extensive deep burns of the БПБ-1U degree were 152 people.

30 Β πeρvοy φaze ρanevοgο προtsessa in πeρiοd τοκsemii 75 bοlnyχ of 102 treatment bylο eφφeκτivnym: πρimene- of πρeπaρaτa ποzvοlilο absορbiροvaτ izbyτοκ ρanevοgο οτdelyaεmοgο, οκazaτ προτivοmiκροbnοe deysτvie The available for schuyusya gnοynuyu inφεκtsiyu πρedοτvρaτiτ and its further ρaz-

35 things. Κ On the 25th day, the burnt burns were filled in full with healthy small-sized granulations.

After using the external device, you will receive a fully-functional home automation device. . 95/27517 ΡСΤЛШ94 / 00073

- 23 -

Healing of ρan was started in the middle of the day. For 46 days after the авavma.

Mostly, it was effective to treat locally treated burns in the second place, and / or to reduce the risk of sluggishness. The process of recovery was made healthy for 70-90 days, mainly in the course of 70-90 days.

10 Β οblasτi κaρdiοχiρuρgii πρeπaρaτ was πρimenεn πρi οπeρatsiyaχ imπlanτatsii elεκτροκaρdiοsτimulyaτορa πο ποvοdu aτρiοvεnτρiκulyaρnοy blοκady and sindροma slabοsτi sinusο- vοgο ρiτma / 100 bοlnyχ / πρi οπερatsiyaχ on οτκρyτοm sερdtsε / 40 bοlnyχ /.

15 Β bοlshinsτve cases πρimenenia πρeπaρaτa οτme- chalsya vyρazhenny sτοyκy inτρaοπeρatsiοnny gzmοsτaz and οτsuτsτvie κaκ ρaκniχ, and τaκ ποzdniχ οslοzhnεniϊϊ sο sτο- ροny οπeρatsiοnnοπ ρany / gemaτοm, inφilτρaτοΕ, nagnοe- Nij / chτο sποsοbsτvοvalο usκορeniyu ρelaρaτivnyχ προtsessοv

20 in pane and decrease in treatment cure. When using the machine, it is supplied in the ran and fully absorbed, it protects the machine from the risk of distressing

25 Positive results were also obtained from the use of the disease and the treatment of diseases associated with the incidence of acute ulcers, 44 with acute ulcers, 44. When the medication is placed on the surface of an ulcer or the condition, the majority of patients have large

30 people had an afflictive effect, after 4-6 days, inflammatory ulcers were cleared and the wounds were cleaned, there was an incurring incident that

35 In case of obstructive injuries, it is possible to automatically expose the patient to the spleen, spleen, liver, urinary tract, and other injuries. 95/27517 ΡСΤЯШ94 / 00073

- 24 - suturing by disassembling a hazardous product using a non-sterile dispenser; hemostasis and prophylaxis of gluteal complications. Dates of sickness in patients with δ were reduced by 5–6 days compared to patients who were not treated at all.

The drug was very effective because it was not harmful to the patient / 896 patients /.

For the purpose of hemostasis and infectious diseases

10 lοzhneny πρεπaρaτ was πρimεnεn πρi οκazanii sροchnοy nειϊ- ροχiρuρgichesκο ^": ποmοschi ποsle πeρvichnοy χiρuρgichesκοy οb- ρabοτκi at 260 bοlnyχ with οτκρyτοϊ chερeπnο-mοzgοvοι; τρavmοy / προniκayuschey and neπροniκayuschey / in 160 - with zaκρyτοιϊ cheρεπnο-mοzgοvοϊϊ τρavmοy with removal! internally

On 15 July, in 315 - on-plan operations on the main roadside, in 98 - on-the-go court of malformations.

In 260 sick patients who have relinquished their access to a 48-year-old truck, they have been injured in 90 cases before they have been injured.

20 chesκοy οbρabοτκi were πeρvichnο zagρyazneny from niχ You are a scatters gnοeροdnaya miκροφlορa: most πaτοgenny zοlο-g τisτyιϊ sτaφilοκοκκ, uslοvnο-πaτοgenny eπideρmalnyϊϊ sτaφilοκοκκ, ρezhe - κishechnaya πalοchκa, προτey in edinichnyχ sluchayaχ - sinegnοynaya πalοchκa.

25 These painful things took place at different times from the moment of accident: up to 6 hours - 164 hours, up to 12 hours - 45 people, up to 24 hours - 12 hours, two to four hours days ϊ - 8 people; more than 3 days - 16 people.

30 The use of a sponge in the course of an operative intervention in the form of a quick-repairing device caused an accident in 25 seconds - 2 minutes.

And in one case, out of 833 observations, it was ot-labeled with complications, at the same time as in the online

35 groups / 120 patients / use of conventional methods of treatment, including the parenteral administration of antibiotics, dull complications occurred in all 20 cases

IΧο - 25 -

Pοlοzhiτzlnyε ρεzulτaτy / 100έ / τaκzhe were ποlu- cheny πρi πρimenεnii πρeπaρaτa 63-x bοlnyχ with gnοynymi προtsessaιli cheρeπa and gοlοvnοgο mοzga, κοτορye vοzniκali 5 bοlshinsτve cases οτdalennyε sροκi ποsle cheρeπnο- mοzgοvοϊ-ϊ τρavmy, πρi eτοm οsτeοmπeliτ κοsτey cheρεπa nab- there were 18 patients with pain, abscesses, subdural and internal medications - in 32 patients, and impaired black and cerebral wounds - in 13 patients. 10 In all sick, treated patients, the cerebral wounds healed by acute tension.

In addition to the aforementioned errors: the methodology, the company found widespread use in the practice, and various interventions. When operating 15 times this area, the sponge with a size of 1 x 3 mm is placed on the back of the eyeball near the opening of the vessel.

The results of the inconvenience to the improvement of the condition of the product are shown in Table 8. 20 Table 8

Β Therapies of the negative interventions for the benefit of the beneficiaries, positive outcome,%

1 3 4 ^"

1 extracts of the catarrh

2 Integrated Operations

25 3 Wastewater treatment plants

4 Radical keratomy

5 Sclerosoplasty

6 Properties 30 7 Evistratsia

8 Enucleation

9 Pharmaceuticals, Emulsions, 2δ00 100 Economic Operations

Зδ 10 Deletion of а ля ля 11 11 on the last century

\ УΟ 95/27517 ΡСΤ / ΙШ94 / 00073

- 26 - If there is an outgoing shock / excess of 30,000 observations /, it will cause rapid and / or rapid out-of-control crashes / 100 times, and

The ophthalmic treatment is also used in patients with an easy-to-use glance for the treatment of the illness / 300 patients /. For these cases, the unit is dia. A good way to prevent moisture from the waste heat is because of the favorable conditions for the improvement of the laser. 15 In general, we offer you the best ... of life. Combined hemostatic and antibiotic delivery. With the use of charging, reliable and effective effect is achieved within 0.5-2 minutes. It is also effective for incidents of 20 cases / inconsequential complications and for the treatment of indefinitely differentiated values. The duration of the drug is stored for up to 14 days.

Β ^~ 2% of cases were found to be positive 25% of the total number of cases. Dοsτigaemye deιϊsτviya πρeπa- ρaτa οbuslοvleny egο sποsοbnοsτyu samοsτοyaτelnο ρassa- syvaτsya in ορganizme, πρi eτοm in ρane sοzdaeτsya delο anti τibiοτzha, κοτορyιϊ πο meρe ρassasyvaniya πρεπaρaτa ποsτu- πaeτ in ρanu, sοzdavaya vysοκie anτibaκτeρialnyε κοntsenτ- 30 ρatsii in οτnοshenii οsnοznοn ρanevοy miκροφlορy.

The proposed product is also available with a high quality access / facilitates the process of repairing and / or ensuring that it is free of any problems. Thanks to the good practices, the drug is stimulated by the therapeutic processes in Rana and the healing _-: it healing.

Pρεπaρaτ mοzhετ byτ πρimenen zο vseχ οblasτyaχ ^χ iρuρ nologies. It is absolutely harmless and does not fail in any other way. 17 ΡСΤ / ΙШ94 / 00073

- 27 -

Disposal of the appliance - 2 years for a small room, it is preferable in the dark.

The method of receiving the product allows you to receive the device with a high output / 90% /. It doesn’t require a dicarious raw materials, environmentally friendly.

The method of treating a patient with a medical or non-surgical antibiotic in the following cases is negligible

First, they prepare the products of the desired gelatin in faracilin by a well-known method. For this purpose, a large amount of gelatin is added to the preparation of faracilin for its swelling. The resulting mixture is heated at a temperature of 8δ-90 ° C for a complete degradation of the iron. The resulting solution cools and mixes it with other products Calcium chloride, phosphate and sulphide. Then, the mixture is placed in a container equipped with a stirrer, and defoamed for the production of foam volume, which rises above the initial volume by at least 2 times. Then, in the process of foaming into the developing foam, the antibiotic-aqueous solution of the gene of sulfate is added.

Received it is poured into cuvettes and placed in a mobile camera. Foam is frozen and the selected ρε is pressed. Then the cuvettes with the freezer are placed in a vacuum-drying apparatus, where it doubles the freeze-dried κε. After this, the resulting product in the form of a plate is partly cut in size, it is packaged, in general, there is a risk of inconvenience to it.

For better understanding of the invention, the following specific examples are provided.

For example, 1.

Received treatment of the following composition

gelatin 64, 5 furacilin 0.263 calcium chloride 0.16 gentamycin sulfate 19.2 - 28 - sulphon 0.86 water 1 δ

Concentrate consisting of δδθ ml of distilled 5 water, 0.098 g of furacilin and 24 g of calcined gelatin, taken up 0.6 ml of 10% calcium sulfate, 0% sulphide, 0% sulphide, 0% - burning dry matter 0.9378 g in 5 ml of distilled water. Pοluchennuyu mixture ποmeschayuτ in emκοsτ equipped meshalκοy 10 and vsπenivayuτ dο ποlucheniya οbema πeny, πρevy- shayuschegο πeρΕθnachalny οbem mixture 2 ρaza and zaτem οchen medlennο malenκοy sτρuyκοy πρi ποsτοyannοm vsπe- nivanii vvοdyaτ 50 ml vοdnοgο ρasτvορa genτamitsina sulfonic φaτa / sοdερzhanie genτamitsina sulphate of 7.148 g in 50 ml of 5 distilled water. The resulting foam is quickly discharged into cooled cuvettes and placed in a mobile closure, where the temperature is minus 60 ° C, and it lasts 4 times. Under these conditions, the foam is frozen with the formation of a crystalline structure. Subsequently, suites with 20 refrigerated ports are placed in a vacuum dryer, which is less than 40 ° C / 38 ° C dry / minus 40 ° C. The product received cuts into the plates the necessary values, packs them with plastic and stores 25 lyses with the radiative method. For example, 2.

There is a cure for the following composition / m.s.%/: gelatin 64.6

30 Furacilin 0.22 Calcium Chloride 0.199 Gentamycin Sulfate 18.51 Sulfon 1, 347 Water 1 δ

Diluent 3, composed of δδθ ml of dostilled water, 0.088 g of furacilin and 26 g of calcined zhlatin, 0.8 ml of 10 ml of calcium sulfate / water is diluted with 1.2 ml of hydrochloride, - zhanyz dry weight 1.5 g s 8 ml distilled

dy. The resulting mixture is placed in a container equipped with a mixer .., ^~ it is possible to obtain the best value, which exceeded the initial volume of the mixture by 2.5 times. Β πο- 5 Lucheng πenu zvοdyaτ, κaκ uκazanο in πρimeρε 1 vοdnyιϊ ρasτvορ gεnτamitsina sul ^ aτa / 7,488 g in genτamitsΕκa sulfonic φaτa δθ ml disτπllϊφοvannοϊ: vοdyΛ Zamορazhivanπe πe- us οsuschesτvlyayuτ πρi τemπeρaτuρz minus δ8 ° C of 5 τechεnie suτοκ. Freeze-drying is carried out at a temperature of 10 hours at 35 ° C. Drying cycle - 38 hours. Further operations with the resulting product exist, as in the case of Example 1. Example 3.

Received medical treatment of the following composition 15 (w / w%) /: gelatin 70.05 furacilin 0.225 calc /

20 sulphon 2.25 water 10.0

A solution consisting of 550 ml of distilled water, 0.090 g of furacilin and 28 g of dried yellow, is taken up as 1 ml of 10% calcium chloride, 1 ml g in 7 ml of distributed water. Pοluchennuyu mixture ποmeschayuτ in emκοsτ equipped mεshal- κοy and vsπeκivayuτ dο ποlucheniya οbεma πεny, πρevyshayuschε- gο περzοnachalny οbem mixture of 3 ρaza, Β ποluchennuyu 30 πenu vvοdyaτ, κaκ uκazanο in πρimερε Ι.vοdny ρasτvορ gεnτamitsina sulώaτa / 6,880 g in genτamitsina sulφaτa 50 ml of distilled water. Foam freezing is essentially πρ :: temperature minus 56 ° C for 4 days. Freeze-drying is carried out at a temperature of 35 ° C to 38 ° C / drying cycle 36 hours /. Further developments with an excellent product exist, as in Example 1.

Ρπmερ 4.

The best way to do this is to \ UΟ 95/27517

- 30 - /mas.%/: zhelatin 75 souracilin 0.3

5 Calcium Chloride 0; 3 Gentamicin Sulphate 12 Sulphonyl 2.4 Water 10.0

Concentrated with δδϋ ml of distilled 10 water, 0.12 g of furacilin and 30 g of heated gelatin, 1.2 ml of 10% calcium sulfate, diluted with calcium chloride, and the content of dry matter 1,1254 g in 6 ml of distilled water /. The resulting mixture is placed in a container equipped with a 15 female stirrer, and defoamed to increase the volume of foam, which increases the initial volume of the mixture 2 times. The resulting foam was administered, as indicated in Example 1, the aqueous solution of gamma-volumine sulphate / 4.8 g of gentamicin sulphate was diluted in 50 ml of the dispersion. Freezing of foam has a temperature of minus 60 ° С for 4 days. Freeze-drying is controlled at a temperature of minus 45 ° C to 40 ° C / drying cycle of the δδ clock /. Further operations with the resulting product: .. of course, as in Example 1. 2δ Intended use

Medication can be used for all areas of arthritis - general chirurgia, neuropathy, cerebrovascular disease, and irregular disease.

Claims

\ УΟ 95/27517 ΡСΤЛШ94 / 00073

- 31 - SUMMARY ... L30BΡZΡ2ΗS STREET 1. Therapeutic treatment of anesthetics and antibiotics for local treatment, a patient who is a non-obstructing patient In addition, it also contains gentamycin sulfate in the following case, in wt.%: gelatin 60-80

10 Furacilin 0.1-0.4 Calcium Chloride 0.1-0.4 Gentamycin Sulphate 10.0-30.0 Sulphon 0.7-3, other water

15 2. Sποsοb ποlucheniya lεchεbnοgο πρeπaρaτa πο π.1, vκlyuchayuschy mixing ρasτvορa zhelaτina, vοdnyχ ρasτvο- ροv φuρatsilina, κaltsiya χlορida, φορmaldegida, sulφο- nοla, vslenivaniε ποluchennοy mixture sushκu and sτeρiliza- tion, χ aρ aκ τ ε ρ and s uyuschi nd with I τεm, chτο πρi 20 smεshivanii isποlzuyuτ ρasτvορ προκalennοgο zhεlaτina in vοdnοm ρasτvορe φuρatsilina, vsπenivanie mixture οsuschesτvlya- yuτ dο ποlucheniya πεny οbemοm, πρevyshayuschim πeρvοnachalny οbem mixture of not less than 2 ρaza, ποsle chegο in προtses- se dalnεishεgο vsπεnivaniya in I take up the water supply 25 gεn amitsina sulφaτa, περεd sushκοκ ποluchennuyu πenu za- mορazhivayuτ πρπ τemπeρaτuρe not higher than minus 50 ° C for φορ- miροvaniya κρisτallichesκοy sτρuκτuρy προduκτa, sushκu πο- luchεnnοgο προduκτa οsuschεsτvlyayuτ meτοdοm sublimation πρi nachalnοy τemπeρaτuρe -50 - -30 ° C and 30 κοnechnοy τemπeρaτuρe 38- 40 ° C, and sterilization is carried out by means of radiative irradiation, and thus the ratio of components in the mixture is as follows in wt. : calcified gelatin 3.5-6.0 furacilin 0.005-0.03

35 Calcium chloride 0.005-0.03 sulfon 0.04-0.25 gentamycin 0.6-2.5 φορ maldehyde 0.05-0.09 other water.