WO1995027047A2 - Highly-purified recombinant reverse transcriptase - Google Patents

Highly-purified recombinant reverse transcriptase Download PDF

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Publication number
WO1995027047A2
WO1995027047A2 PCT/US1995/004092 US9504092W WO9527047A2 WO 1995027047 A2 WO1995027047 A2 WO 1995027047A2 US 9504092 W US9504092 W US 9504092W WO 9527047 A2 WO9527047 A2 WO 9527047A2
Authority
WO
WIPO (PCT)
Prior art keywords
reverse transcriptase
highly
purified recombinant
expression
recombinant reverse
Prior art date
Application number
PCT/US1995/004092
Other languages
French (fr)
Other versions
WO1995027047A3 (en
Inventor
Daniel Louis Kacian
Michael Garth Riggs
James Garfield Putnam
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Priority to CA002186018A priority Critical patent/CA2186018C/en
Priority to JP52589995A priority patent/JP3808501B2/en
Priority to KR1019960705427A priority patent/KR100262258B1/en
Priority to AU22371/95A priority patent/AU696497B2/en
Publication of WO1995027047A2 publication Critical patent/WO1995027047A2/en
Publication of WO1995027047A3 publication Critical patent/WO1995027047A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08—RNA viruses
    • C07K14/14—Reoviridae, e.g. rotavirus, bluetongue virus, Colorado tick fever virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Abstract

A plasmid for expression of Moloney Murine Leukemia Virus-dirived reverse transcriptase in E. Coli cells deficient in the expression of indigenous RNAse activity, a method for purification of the recombinant enzyme, and a composition comprising a cloned and purified reverse transcriptase optimized for use in cDNA and nucleic acid amplification procedures.
PCT/US1995/004092 1994-04-01 1995-03-29 Highly-purified recombinant reverse transcriptase WO1995027047A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002186018A CA2186018C (en) 1994-04-01 1995-03-29 Highly-purified recombinant reverse transcriptase
JP52589995A JP3808501B2 (en) 1994-04-01 1995-03-29 Highly purified recombinant reverse transcriptase
KR1019960705427A KR100262258B1 (en) 1994-04-01 1995-03-29 Highly purified recombinant reverse transcriptase
AU22371/95A AU696497B2 (en) 1994-04-01 1995-03-29 Highly-purified recombinant reverse transcriptase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22180494A 1994-04-01 1994-04-01
US08/221,804 1994-04-01

Publications (2)

Publication Number Publication Date
WO1995027047A2 true WO1995027047A2 (en) 1995-10-12
WO1995027047A3 WO1995027047A3 (en) 1995-10-26

Family

ID=22829460

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1995/004092 WO1995027047A2 (en) 1994-04-01 1995-03-29 Highly-purified recombinant reverse transcriptase

Country Status (10)

Country Link
US (2) US5935833A (en)
EP (1) EP0688870B1 (en)
JP (1) JP3808501B2 (en)
KR (1) KR100262258B1 (en)
AT (1) ATE242811T1 (en)
AU (1) AU696497B2 (en)
CA (1) CA2186018C (en)
DE (1) DE69531023T2 (en)
ES (1) ES2199967T3 (en)
WO (1) WO1995027047A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5244797B1 (en) * 1988-01-13 1998-08-25 Life Technologies Inc Cloned genes encoding reverse transcriptase lacking rnase h activity
US6120605A (en) * 1998-02-05 2000-09-19 Asm Japan K.K. Semiconductor processing system
DE10046960A1 (en) 2000-09-22 2002-04-11 Roche Diagnostics Gmbh Process for the production of an active, heterodimeric AMW-RT in prokaryotic cells
US20030211970A1 (en) * 2001-06-01 2003-11-13 Samuel Nochumson Processing of plasmid-containing fluids
US20030027196A1 (en) * 2001-08-02 2003-02-06 Barnes Wayne M. Magnesium precipitate methods for magnesium dependent enzymes
DE10315640A1 (en) 2003-04-04 2004-10-14 Ignatov, Konstantin Process for the controlled release of components into a solution
KR100437889B1 (en) * 2003-08-26 2004-07-07 주식회사 인트론바이오테크놀로지 Reverse transcriptase of Reticuloendotheliosis Virus and method for production thereof
US7595179B2 (en) * 2004-04-19 2009-09-29 Applied Biosystems, Llc Recombinant reverse transcriptases
GB2416352B (en) * 2004-07-21 2009-01-28 Bioline Ltd A method for performing the hot start of enzymatic reactions
JP5279339B2 (en) * 2008-05-16 2013-09-04 タカラバイオ株式会社 Composition for reverse transcription reaction
CN104250642A (en) * 2014-04-30 2014-12-31 厦门安普利生物工程有限公司 Method for extracting and purifying M-MLV reverse transcriptase

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4943531A (en) * 1985-05-06 1990-07-24 The Trustees Of Columbia University In The City Of New York Expression of enzymatically active reverse transcriptase
US5017492A (en) * 1986-02-27 1991-05-21 Life Technologies, Inc. Reverse transcriptase and method for its production

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4943531A (en) * 1985-05-06 1990-07-24 The Trustees Of Columbia University In The City Of New York Expression of enzymatically active reverse transcriptase
US5017492A (en) * 1986-02-27 1991-05-21 Life Technologies, Inc. Reverse transcriptase and method for its production

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
J. BIOL. CHEM., vol. 267, no. 23, 15 August 1992 pages 16015-16018, KELLY K. O. ET AL. 'RNase PH is essential for the tRNA processing and viability in RNase-deficient Escherichia coli cells ' *
PROC. NATL. ACAD. SCI. USA, vol. 76, no. 11, November 1979 pages 5774-5778, HAUTALA J. A. ET AL. 'Increased expression of a eukaryotic gene in Escherichia coli through stabilization of its messenger RNA ' *

Also Published As

Publication number Publication date
ATE242811T1 (en) 2003-06-15
EP0688870B1 (en) 2003-06-11
JPH09508806A (en) 1997-09-09
ES2199967T3 (en) 2004-03-01
DE69531023T2 (en) 2004-05-06
AU2237195A (en) 1995-10-23
CA2186018C (en) 2002-02-12
KR100262258B1 (en) 2000-07-15
KR970702362A (en) 1997-05-13
AU696497B2 (en) 1998-09-10
CA2186018A1 (en) 1995-10-12
EP0688870A1 (en) 1995-12-27
JP3808501B2 (en) 2006-08-16
DE69531023D1 (en) 2003-07-17
WO1995027047A3 (en) 1995-10-26
US5998195A (en) 1999-12-07
US5935833A (en) 1999-08-10

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