WO1995025806A3 - Packaging-deficient lentiviruses - Google Patents

Packaging-deficient lentiviruses Download PDF

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Publication number
WO1995025806A3
WO1995025806A3 PCT/GB1995/000663 GB9500663W WO9525806A3 WO 1995025806 A3 WO1995025806 A3 WO 1995025806A3 GB 9500663 W GB9500663 W GB 9500663W WO 9525806 A3 WO9525806 A3 WO 9525806A3
Authority
WO
WIPO (PCT)
Prior art keywords
packaging
lentiviruses
deficient
deficient lentiviruses
virus
Prior art date
Application number
PCT/GB1995/000663
Other languages
French (fr)
Other versions
WO1995025806A2 (en
Inventor
Andrew Michael Lindsay Lever
Geoffrey Philip Harrison
Eric Hunter
Original Assignee
Syngenix Ltd
Lever Andrew M L
Geoffrey Philip Harrison
Eric Hunter
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB9405876A external-priority patent/GB9405876D0/en
Priority claimed from GB9405875A external-priority patent/GB9405875D0/en
Priority claimed from GBGB9425026.3A external-priority patent/GB9425026D0/en
Application filed by Syngenix Ltd, Lever Andrew M L, Geoffrey Philip Harrison, Eric Hunter filed Critical Syngenix Ltd
Priority to JP7524505A priority Critical patent/JPH09510361A/en
Priority to EP95912349A priority patent/EP0752000A1/en
Publication of WO1995025806A2 publication Critical patent/WO1995025806A2/en
Publication of WO1995025806A3 publication Critical patent/WO1995025806A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15051—Methods of production or purification of viral material
    • C12N2740/15052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Packaging-deficient lentiviruses are described. In particular, a vector is capable of producing proteins of a virus selected from MVV (Maedi-Visna Virus) and HIV-2, but not of packaging the viral RNA.
PCT/GB1995/000663 1994-03-24 1995-03-24 Packaging-deficient lentiviruses WO1995025806A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP7524505A JPH09510361A (en) 1994-03-24 1995-03-24 Packaging defective lentivirus
EP95912349A EP0752000A1 (en) 1994-03-24 1995-03-24 Packaging-deficient lentiviruses

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GB9405876.5 1994-03-24
GB9405876A GB9405876D0 (en) 1994-03-24 1994-03-24 Modified virus and its use as a vector
GB9405875A GB9405875D0 (en) 1994-03-24 1994-03-24 Recombinant molecules and their use in gene transfer
GB9405875.7 1994-03-24
GBGB9425026.3A GB9425026D0 (en) 1994-12-09 1994-12-09 Packaging-defective vectors
GB9425026.3 1994-12-09

Publications (2)

Publication Number Publication Date
WO1995025806A2 WO1995025806A2 (en) 1995-09-28
WO1995025806A3 true WO1995025806A3 (en) 1996-01-04

Family

ID=27267117

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1995/000663 WO1995025806A2 (en) 1994-03-24 1995-03-24 Packaging-deficient lentiviruses

Country Status (3)

Country Link
EP (1) EP0752000A1 (en)
JP (1) JPH09510361A (en)
WO (1) WO1995025806A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6013516A (en) * 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
ATE298371T1 (en) * 1997-09-24 2005-07-15 Univ California NON-PRIMATE LENTIVIRAL VECTORS AND PACKAGING SYSTEMS
US6790657B1 (en) 1999-01-07 2004-09-14 The United States Of America As Represented By The Department Of Health And Human Services Lentivirus vector system
FR2979919B1 (en) * 2011-09-12 2015-12-11 Centre Nat Rech Scient NON-INTEGRATIVE CHIMERIC LENTIVIRAL GENOMES AS INNOVATIVE VACCINES AGAINST HIV-1

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993017118A2 (en) * 1992-02-28 1993-09-02 Syngenix Limited Defective packaging non-oncoviral vectors based on mpmv and hiv
WO1993020220A1 (en) * 1992-03-27 1993-10-14 Whitehead Institute For Biomedical Research Non-infectious hiv particles and uses therefor
WO1994003622A1 (en) * 1992-07-31 1994-02-17 Imperial College Of Science, Technology & Medicine D-type retroviral vectors, based on mpmv

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993017118A2 (en) * 1992-02-28 1993-09-02 Syngenix Limited Defective packaging non-oncoviral vectors based on mpmv and hiv
WO1993020220A1 (en) * 1992-03-27 1993-10-14 Whitehead Institute For Biomedical Research Non-infectious hiv particles and uses therefor
WO1994003622A1 (en) * 1992-07-31 1994-02-17 Imperial College Of Science, Technology & Medicine D-type retroviral vectors, based on mpmv

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
D.A.M. KONINGS ET AL.;: "Novel GACG- hairpin pair motif in the 5' untranslated region of type C retroviruses related to murine leukemia virus", J. VIROL., vol. 66, pages 632 - 640 *
R.G. VILE ET AL.;: "Identification of a generalised packaging sequence for D-type retroviruses and generation of a D-type retroviral vector", VIROLOGY, vol. 189, pages 786 - 791 *
S. YANG AND H.M. TEMIN;: "A double hairpin structure is necessary for the efficient encapsidation of spleen necrosis virus retroviral DNA", EMBO J., vol. 13, pages 713 - 726 *

Also Published As

Publication number Publication date
JPH09510361A (en) 1997-10-21
WO1995025806A2 (en) 1995-09-28
EP0752000A1 (en) 1997-01-08

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