WO1995024497A3 - Enzymatic preparation of polysaccharides - Google Patents

Enzymatic preparation of polysaccharides Download PDF

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Publication number
WO1995024497A3
WO1995024497A3 PCT/EP1995/000935 EP9500935W WO9524497A3 WO 1995024497 A3 WO1995024497 A3 WO 1995024497A3 EP 9500935 W EP9500935 W EP 9500935W WO 9524497 A3 WO9524497 A3 WO 9524497A3
Authority
WO
WIPO (PCT)
Prior art keywords
polysaccharides
processes
enzymatic preparation
nucleotide
polymannuronan
Prior art date
Application number
PCT/EP1995/000935
Other languages
French (fr)
Other versions
WO1995024497A2 (en
Inventor
Manfred Lansing
Peter Prehm
Michael O'regan
Irene Martini
Original Assignee
Fidia Advanced Biopolymers Srl
Manfred Lansing
Peter Prehm
Regan Michael O
Irene Martini
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fidia Advanced Biopolymers Srl, Manfred Lansing, Peter Prehm, Regan Michael O, Irene Martini filed Critical Fidia Advanced Biopolymers Srl
Priority to AU20699/95A priority Critical patent/AU2069995A/en
Publication of WO1995024497A2 publication Critical patent/WO1995024497A2/en
Publication of WO1995024497A3 publication Critical patent/WO1995024497A3/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/20—Polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/726—Glycosaminoglycans, i.e. mucopolysaccharides
    • A61K31/728—Hyaluronic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
    • A61L15/28—Polysaccharides or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
    • A61L31/04—Macromolecular materials
    • A61L31/042—Polysaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates

Abstract

Provided are in vitro processes for the production of polysaccharides such as hyaluronic acid, cellulose, polymannuronan, chitin, etc. These processes are carried out under reaction conditions that permit the recycling of nucleotide-phosphates required for the formation of sugar nucleotide precursors employed in the synthesis of these polysaccharides. Also provided are polysaccharides produced via these processes, and pharmaceutical and cosmetic compositions containing these polysaccharides.
PCT/EP1995/000935 1994-03-11 1995-03-10 Enzymatic preparation of polysaccharides WO1995024497A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU20699/95A AU2069995A (en) 1994-03-11 1995-03-10 Enzymatic preparation of polysaccharides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT1994PD000042A IT1268954B1 (en) 1994-03-11 1994-03-11 PROCESS FOR THE PREPARATION OF HYALURONIC ACID BY MEANS OF ENZYMATIC SYNTHESIS AND RELATED PHARMACEUTICAL COMPOSITIONS
ITPD94000042 1994-03-11

Publications (2)

Publication Number Publication Date
WO1995024497A2 WO1995024497A2 (en) 1995-09-14
WO1995024497A3 true WO1995024497A3 (en) 1995-11-16

Family

ID=11390593

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1995/000935 WO1995024497A2 (en) 1994-03-11 1995-03-10 Enzymatic preparation of polysaccharides

Country Status (3)

Country Link
AU (1) AU2069995A (en)
IT (1) IT1268954B1 (en)
WO (1) WO1995024497A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9228027B2 (en) 2008-09-02 2016-01-05 Allergan Holdings France S.A.S. Threads of Hyaluronic acid and/or derivatives thereof, methods of making thereof and uses thereof

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6951743B2 (en) 1997-10-31 2005-10-04 University Of Oklahoma Board Of Regents Hyaluronan synthase genes and expression thereof in bacillus hosts
US7091008B1 (en) 1994-07-01 2006-08-15 The Board Of Regents Of The University Of Oklahoma Hyaluronan synthase genes and expression thereof in Bacillus hosts
US6455304B1 (en) 1994-07-01 2002-09-24 The Board Of Regents Of The University Of Oklahoma Hyaluronate synthase gene and uses thereof
WO1997020061A1 (en) * 1995-11-30 1997-06-05 The Scripps Research Institute Synthesis of hyaluronic acid
IT1282219B1 (en) * 1995-12-20 1998-03-16 Fidia Advanced Biopolymers Srl COMBINED CHEMICAL-PHYSICAL PROCESS FOR THE PREPARATION OF LOW MOLECULAR WEIGHT HYALURONIC ACID FRACTIONS CHARACTERIZED BY LOW
EP0861903A1 (en) * 1997-02-22 1998-09-02 Lansing, Manfred Method for reversible immobilizing oligo and/or polysaccharides
CN101113436B (en) 1997-10-31 2013-02-06 俄克拉何马大学董事会 Hyaluronan synthase gene and uses thereof
AU762036B2 (en) 1997-10-31 2003-06-19 Board Of Regents Of The University Of Oklahoma, The Hyaluronan synthase gene and uses thereof
US7223571B2 (en) 1998-04-02 2007-05-29 The Board Of Regents Of The Universtiy Of Oklahoma Targeted glycosaminoglycan polymers by polymer grafting and methods of making and using same
US6444447B1 (en) 1998-11-11 2002-09-03 The Board Of Regents Of The University Of Oklahoma Polymer grafting by polysaccharide synthases
AU774722B2 (en) * 1998-04-02 2004-07-08 Board Of Regents Of The University Of Oklahoma, The Polymer grafting by polysaccharide synthases
US7060469B2 (en) 1998-04-02 2006-06-13 The Board Of Regents Of The University Of Oklahoma Polymer grafting by polysaccharide synthases
US20060188966A1 (en) 1998-04-02 2006-08-24 Deangelis Paul L Natural, chimeric and hybrid glycosaminoglycan polymers and methods of making and using same
US6987023B2 (en) 1998-04-02 2006-01-17 The Board Of Regents Of The University Of Oklahoma DNA encoding hyaluronan synthase from Pasteurella multocida and methods of use
US7094581B2 (en) 1998-10-26 2006-08-22 The Board Of Regents Of The University Of Oklahoma Hyaluronan synthases and methods of making and using same
US7642071B2 (en) 1999-04-01 2010-01-05 The Board Of Regents Of The University Of Oklahoma Methods of expressing gram-negative glycosaminoglycan synthase genes in gram-positive hosts
EP1189643A1 (en) * 1999-06-30 2002-03-27 Novartis AG Ophthalmic molding
US7534589B2 (en) 1999-11-10 2009-05-19 The Board Of Regents Of The University Of Oklahoma Polymer grafting by polysaccharide synthases
KR100501584B1 (en) 2000-02-03 2005-07-18 (주)케이비피 Process for preparing low molecular polymannuronate, a novel use thereof as controller of serum lipids, and functional foods and health-aid foods comprising the same
ITPD20020064A1 (en) 2002-03-12 2003-09-12 Fidia Advanced Biopolymers Srl FOREIGN DERIVATIVES OF HYALURONIC ACID FOR THE PREPARATION OF HYDROGELD FOR USE IN THE BIOMEDICAL, SANITARY AND SURGICAL FIELD AND AS A SYSTEM
WO2004033472A1 (en) * 2002-10-09 2004-04-22 Westfälische Wilhelms-Universität Münster Method for producing monomer uronic acids, especially d- mannuronic acid and d-guluronic acid and derivatives thereof, and the use of the same as medicaments
DE10247073B4 (en) * 2002-10-09 2010-08-05 Mirshafiey, Abbas, Prof. Dr. Process for the preparation of monomeric uronic acids, in particular D-mannuronic acid and D-guluronic acid and their use as medicaments
JP2011503047A (en) 2007-11-13 2011-01-27 バイオ−テクノロジー ゼネラル（イスラエル）リミテッド Dilution filtration sterilization process for viscoelastic biopolymers
ITMI20072348A1 (en) * 2007-12-17 2009-06-18 Medichem Srl METHOD FOR CELL RIGENERATION OF HYALURONIC ACID AND ITS COSMETIC COMPOSITION
CN111676258A (en) * 2020-07-13 2020-09-18 南京恒道医药科技有限公司 Method for continuously preparing and separating sodium hyaluronate

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0144019A2 (en) * 1983-11-25 1985-06-12 Miles Inc. Ultrapure hyaluronic acid and method of making it
WO1992016640A1 (en) * 1991-03-18 1992-10-01 The Scripps Research Institute Oligosaccharide enzyme substrates and inhibitors: methods and compositions
WO1993008205A1 (en) * 1991-10-15 1993-04-29 The Scripps Research Institute Production of fucosylated carbohydrates by enzymatic fucosylation synthesis of sugar nucleotides; and in situ regeneration of gdp-fucose
WO1994001468A1 (en) * 1992-07-03 1994-01-20 M.U.R.S.T., Italian Ministry For Universities And Scientific And Technological Research Hyaluronic acid and derivatives thereof in interpenetrating polymer networks (ipn)

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0144019A2 (en) * 1983-11-25 1985-06-12 Miles Inc. Ultrapure hyaluronic acid and method of making it
WO1992016640A1 (en) * 1991-03-18 1992-10-01 The Scripps Research Institute Oligosaccharide enzyme substrates and inhibitors: methods and compositions
WO1993008205A1 (en) * 1991-10-15 1993-04-29 The Scripps Research Institute Production of fucosylated carbohydrates by enzymatic fucosylation synthesis of sugar nucleotides; and in situ regeneration of gdp-fucose
WO1994001468A1 (en) * 1992-07-03 1994-01-20 M.U.R.S.T., Italian Ministry For Universities And Scientific And Technological Research Hyaluronic acid and derivatives thereof in interpenetrating polymer networks (ipn)

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ICHIKAWA ET AL.: "Enzyme-catalysed oligosaccharide synthesis", ANALYTICAL BIOCHEMISTRY, vol. 202, pages 215 - 238 *
PREHM P.: "Synthesis of hyaluronate in differentiated teratocarcinoma cells : characterization of the synthase", BIOCHEM. J., vol. 211, pages 181 - 189 *
STOOLMILLER ET AL.: "The biosynthesis of hyaluronic acid by Streptococcus", J. BIOL. CHEM., vol. 244, no. 2, 25 January 1969 (1969-01-25), pages 236 - 246 *
SUGAHARA ET AL.: "Biosynthesis of hyaluronic acid by Streptococcus", J. BIOL. CHEM., vol. 254, no. 14, 25 July 1979 (1979-07-25), pages 6252 - 6261 *
WONG ET AL.: "Enzyme-catalyzed synthesis of N-acetyllactosamine with in-situ regeneration of uridine-5'-diphosphate-glucose and uridine-5'-diphosphate galactose", J. ORG. CHEM., vol. 47, pages 5416 - 5418 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9228027B2 (en) 2008-09-02 2016-01-05 Allergan Holdings France S.A.S. Threads of Hyaluronic acid and/or derivatives thereof, methods of making thereof and uses thereof

Also Published As

Publication number Publication date
WO1995024497A2 (en) 1995-09-14
AU2069995A (en) 1995-09-25
ITPD940042A0 (en) 1994-03-11
IT1268954B1 (en) 1997-03-18
ITPD940042A1 (en) 1995-09-11

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