WO1995011305A3 - Insecticidal proteins - Google Patents

Insecticidal proteins Download PDF

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Publication number
WO1995011305A3
WO1995011305A3 PCT/GB1994/002274 GB9402274W WO9511305A3 WO 1995011305 A3 WO1995011305 A3 WO 1995011305A3 GB 9402274 W GB9402274 W GB 9402274W WO 9511305 A3 WO9511305 A3 WO 9511305A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
insecticidal
chimeric
venom
endotoxin
Prior art date
Application number
PCT/GB1994/002274
Other languages
French (fr)
Other versions
WO1995011305A2 (en
Inventor
Susan Ely
Original Assignee
Zeneca Ltd
Susan Ely
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zeneca Ltd, Susan Ely filed Critical Zeneca Ltd
Priority to AU79423/94A priority Critical patent/AU7942394A/en
Publication of WO1995011305A2 publication Critical patent/WO1995011305A2/en
Publication of WO1995011305A3 publication Critical patent/WO1995011305A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10—Animals; Substances produced thereby or obtained therefrom
    • A01N63/16—Arachnids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43522—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from scorpions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Virology (AREA)
  • Insects & Arthropods (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Agronomy & Crop Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Chimeric insecticidal proteins comprise at least part of a Bacillus thuringiensis δ-endotoxin fused to a venom-derived insecticidal protein, such as the AaHIT peptide obtainable from Androctonus australis Hector. The δ-endotoxin portion protects the venom-derived protein and delivers it to the insect gut. DNA constructs encoding such chimeric proteins may be used to express said proteins in biological organisms. Exposure of insects to the chimeric insecticidal proteins is achieved through application to plants of an insecticidal composition containing said proteins or through expression of said proteins within transgenic plants.
PCT/GB1994/002274 1993-10-18 1994-10-18 Insecticidal proteins WO1995011305A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU79423/94A AU7942394A (en) 1993-10-18 1994-10-18 Insecticidal proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB939321469A GB9321469D0 (en) 1993-10-18 1993-10-18 Insecticidal proteins
GB9321469.0 1993-10-18

Publications (2)

Publication Number Publication Date
WO1995011305A2 WO1995011305A2 (en) 1995-04-27
WO1995011305A3 true WO1995011305A3 (en) 1995-05-11

Family

ID=10743726

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1994/002274 WO1995011305A2 (en) 1993-10-18 1994-10-18 Insecticidal proteins

Country Status (3)

Country Link
AU (1) AU7942394A (en)
GB (1) GB9321469D0 (en)
WO (1) WO1995011305A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPM885194A0 (en) * 1994-10-14 1994-11-10 Council Of The Queensland Institute Of Medical Research, The Synthetic peptides and vaccines comprising same
FR2767537B1 (en) * 1997-08-20 2001-07-13 Rhone Poulenc Agrochimie GENE ENCODING ANDROCTONIN, VECTOR CONTAINING SAME AND OBTAINED TRANSFORMED PLANTS RESISTANT TO DISEASE
EP1123398B1 (en) * 1998-10-16 2005-08-10 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Molecular pathogenicide mediated plant disease resistance
US6600019B2 (en) 2000-01-06 2003-07-29 Curagen Corporation Polypeptides and nucleic acids encoding same

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0340948A1 (en) * 1988-04-28 1989-11-08 Mycogen Corporation Novel hybrid pesticidal toxins
EP0374753A2 (en) * 1988-12-19 1990-06-27 American Cyanamid Company Insecticidal toxines, genes coding therefor, antibodies binding them, transgenic plant cells and plants expressing these toxines
EP0431829A1 (en) * 1989-11-29 1991-06-12 Agracetus, Inc. Insecticidal toxins in plants
WO1991017254A1 (en) * 1990-05-03 1991-11-14 The Regents Of The University Of California Method and means for extending the host range of insecticidal proteins

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0340948A1 (en) * 1988-04-28 1989-11-08 Mycogen Corporation Novel hybrid pesticidal toxins
EP0374753A2 (en) * 1988-12-19 1990-06-27 American Cyanamid Company Insecticidal toxines, genes coding therefor, antibodies binding them, transgenic plant cells and plants expressing these toxines
EP0431829A1 (en) * 1989-11-29 1991-06-12 Agracetus, Inc. Insecticidal toxins in plants
WO1991017254A1 (en) * 1990-05-03 1991-11-14 The Regents Of The University Of California Method and means for extending the host range of insecticidal proteins

Also Published As

Publication number Publication date
WO1995011305A2 (en) 1995-04-27
AU7942394A (en) 1995-05-08
GB9321469D0 (en) 1993-12-08

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