WO1995002605B1 - Sex-specific dna probe for parrots, methods and kits - Google Patents
Sex-specific dna probe for parrots, methods and kitsInfo
- Publication number
- WO1995002605B1 WO1995002605B1 PCT/US1994/008023 US9408023W WO9502605B1 WO 1995002605 B1 WO1995002605 B1 WO 1995002605B1 US 9408023 W US9408023 W US 9408023W WO 9502605 B1 WO9502605 B1 WO 9502605B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- probe
- recited
- labeled
- oligonucleotide probe
- parrot
- Prior art date
Links
- 241000287530 Psittaciformes Species 0.000 title claims abstract 4
- 239000000523 sample Substances 0.000 title claims 11
- 229920003013 deoxyribonucleic acid Polymers 0.000 title claims 5
- 239000003298 DNA probe Substances 0.000 claims abstract 6
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims 15
- 239000002751 oligonucleotide probe Substances 0.000 claims 15
- 241000287531 Psittacidae Species 0.000 claims 10
- 210000000349 Chromosomes Anatomy 0.000 claims 5
- 108020003215 DNA Probes Proteins 0.000 claims 3
- 108020004518 RNA Probes Proteins 0.000 claims 3
- 239000003391 RNA probe Substances 0.000 claims 3
- RWQNBRDOKXIBIV-UHFFFAOYSA-N Thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- 229920001850 Nucleic acid sequence Polymers 0.000 claims 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N Rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 claims 1
- 229940113082 Thymine Drugs 0.000 claims 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical group N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims 1
- 238000004166 bioassay Methods 0.000 claims 1
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 1
- 229960002685 biotin Drugs 0.000 claims 1
- 235000020958 biotin Nutrition 0.000 claims 1
- 239000011616 biotin Substances 0.000 claims 1
- 230000001488 breeding Effects 0.000 abstract 1
Abstract
Novel DNA probes for determining the sex of parrots in captive breeding programs and in wild populations are disclosed. The unique DNA probes of the present invention are sex specific, but not parrot-species specific. In addition, the DNA probes of the present invention are reliable, yield results within approximately 24 hours, easy to use, and are not harmful to the parrots being tested.
Claims
AMENDED CLAIMS
[received by the International Bureau on 20 December 1994 (20.12.94); original claims 1-18 replaced by new claims 1-17 (5 pages)]
(1) An oligonucleotide probe having an effective number of nucleotides for hybridizing specifically with a DΝA segment which is characteristic for female parrots of the Psittacidae genus, the DΝA segment being located on the chromosome of the female parrot of the Psittacidae genus.
(2) An oligonucleotide probe as recited in claim
1, said probe further including a labeled moiety so that, when said labeled probe is hybridized to the DΝA segment, the DΝA segment on the chromosome can be detected.
(3) An oligonucleotide probe as recited in claim
2, said labeled moiety being a fluorophore.
(4) An oligonucleotide probe as recited in claim
3, said fluorophore being selected from a group consisting of a fluoroscein, a rhodamine or any other fluorophore.
(5) An oligonucleotide probe as recited in claim 2, said labeled moiety being selected from a group consisting of biotin and a radioisotope.
(6) An oligonucleotide probe as recited in claim 1, said probe having a periodicity of groups of about 2 to about 6 thymine or adenine residues, said groups being repeated on average of about 10.5 nucleotides.
(7) An oligonucleotide probe as recited in claim 1, said oligonucleotide probe further having a curved DNA element.
(8) An oligonucleotide probe as recited in claim 1, said oligonucleotide probe having a nucleotide sequence selected from a group having the following 5'-3' sequence or 3'-5' sequence or an equivalent sequence or an effective segment thereof:
5'-GTTTTCGTCC ATTCCTAACC ACATTAAAGC CTATTTTTCA CCCATTTCCA 3'-CAAAAGCAGG TAAGGATTGG TGTAATTTCG GATAAAAAGT GGGTAAAGGT
ACCAATTTTA AGCAATTTGT GGTCATTTCA AACACAGTTT TCACCACTTT TGGTTAAAAT TCGTTAAACA CCTGTAAAGT TTGTGTCAAA AGTGGTGAAA
GAACAAGCTT TAAGTCCTTT TTGGTTGCTT GTAAATGATT TTTGGAGTTT CTTGTTCGAA ATTCAGGAAA AACCAAGCAA CATTTACTAA AAACCTCAAA
TCTAACCCCT TTTGAGCCAT TTTTTCTGTT TCTAACCCAT TTTTTCAACA AGATTGGGGA AAACTCGGTA AAAAAGACAA AGATTGGGTA AAAAAGTTGT
GTTCTAGCTC GGTTTAAGTA GTTTTTGCTT TTTTCTAACC CATTGATCCC CAAGATCGAG CCAAATTCAT CAAAAACGAA AAAAGATTGG GTAACTAGGG
ATGACTAATC AGTTTTAAGC CGTTTTTGTC CATTTCTGAC CCATCTTTGC TACTGATTAG TCAAAATTCG GCAAAAACAG GTAAAGACTG GGTAGAAACG
CCAGCTCTAG CTTTGTTTAA GCCGTTTTTC TCCATTTCTA ACCCGTTTCT GGTCGAGATC GAAACAAATT CGGCAAAAAG AGGTAAAGAT TGGGCAAAGA
AGCCCATTCC TGACCTGTTT TAAGCCTATT CCTAACCCAT TTCCAACCCA TCGGGTAAGG ACTGGACAAA ATTCGGATAA GGATTGGGTA AAGGTTGGGT
TTTTTGGTCT TGTCAAATGC ATTTTTCACC TCTTCTGACT CGCTTGAAGA AAAAACCAGA ACAGTTTACG TAAAAAGTGG AGAAGACTGA GCGAACTTCT
CCTTTTTGCC G-3' GGAAAAACGG C-5'
(9) An oligonucleotide probe as recited in claim 1, said probe being a DNA probe.
(10) An oligonucleotide probe as recited in claim 1, said probe being a RNA probe.
(11) A method of determining the sex of a parrot of the Psittacidae genus, said method comprising: hybridizing the labeled probe of claim 2 to the female chromosome DNA of the animal; and detecting the labeled probe hybridized to the DNA segment on the female chromosome of the parrot to identify the female chromosome of the parrot.
(12) A method as recited in claim 11, said detecting step comprises: visualizing the labeled probe hybridized to the DNA segment.
(13) A method as recited in claim 11, the labeled probe being a labeled DNA probe.
(14) A method as recited in claim 11, the labeled probe being a labeled RNA probe.
(15) A kit for determining the sex of a parrot of the Psittacidae genus, said kit comprising: a.) an oligonucleotide probe of claim 1; and b.) components for carrying out an assay to determine the sex of the parrot.
(16) A kit as recited in claim 15, said oligonucleotide probe being a DNA probe.
(17) A kit as recited in claim 15, said oligonucleotide probe being a RNA probe.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP94922588A EP0708782A4 (en) | 1993-07-15 | 1994-07-15 | Sex-specific dna probe for parrots, methods and kits |
| AU73645/94A AU7364594A (en) | 1993-07-15 | 1994-07-15 | Sex-specific dna probe for parrots, methods and kits |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US9319893A | 1993-07-15 | 1993-07-15 | |
| US08/093,198 | 1993-07-15 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1995002605A1 WO1995002605A1 (en) | 1995-01-26 |
| WO1995002605B1 true WO1995002605B1 (en) | 1995-02-23 |
Family
ID=22237701
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1994/008023 WO1995002605A1 (en) | 1993-07-15 | 1994-07-15 | Sex-specific dna probe for parrots, methods and kits |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0708782A4 (en) |
| AU (1) | AU7364594A (en) |
| CA (1) | CA2167295A1 (en) |
| WO (1) | WO1995002605A1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AUPN283195A0 (en) * | 1995-05-05 | 1995-06-01 | Australian Water Technologies Pty Ltd | Method for the detection of viable cryptosporidium parvum cells |
-
1994
- 1994-07-15 CA CA002167295A patent/CA2167295A1/en not_active Abandoned
- 1994-07-15 AU AU73645/94A patent/AU7364594A/en not_active Abandoned
- 1994-07-15 EP EP94922588A patent/EP0708782A4/en not_active Withdrawn
- 1994-07-15 WO PCT/US1994/008023 patent/WO1995002605A1/en not_active Application Discontinuation
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