WO1995001420A3 - A method of producing a protein disulfide redox agent - Google Patents

A method of producing a protein disulfide redox agent Download PDF

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Publication number
WO1995001420A3
WO1995001420A3 PCT/DK1994/000264 DK9400264W WO9501420A3 WO 1995001420 A3 WO1995001420 A3 WO 1995001420A3 DK 9400264 W DK9400264 W DK 9400264W WO 9501420 A3 WO9501420 A3 WO 9501420A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein disulfide
redox agent
disulfide redox
producing
iii
Prior art date
Application number
PCT/DK1994/000264
Other languages
French (fr)
Other versions
WO1995001420A2 (en
Inventor
Jens Toenne Andersen
Carsten M Hjort
Boerge Diderichsen
Dorrit Aaslyng
Christian K Hansen
Original Assignee
Novo Nordisk As
Jens Toenne Andersen
Carsten M Hjort
Boerge Diderichsen
Dorrit Aaslyng
Christian K Hansen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As, Jens Toenne Andersen, Carsten M Hjort, Boerge Diderichsen, Dorrit Aaslyng, Christian K Hansen filed Critical Novo Nordisk As
Priority to EP94919568A priority Critical patent/EP0710281A1/en
Priority to JP7503223A priority patent/JPH08511944A/en
Priority to AU70682/94A priority patent/AU7068294A/en
Publication of WO1995001420A2 publication Critical patent/WO1995001420A2/en
Publication of WO1995001420A3 publication Critical patent/WO1995001420A3/en

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q5/00—Preparations for care of the hair
    • A61Q5/04—Preparations for permanent waving or straightening the hair
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/008—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/06—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0051—Oxidoreductases (1.) acting on a sulfur group of donors (1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y108/00—Oxidoreductases acting on sulfur groups as donors (1.8)
    • C12Y108/01—Oxidoreductases acting on sulfur groups as donors (1.8) with NAD+ or NADP+ as acceptor (1.8.1)
    • C12Y108/01008—Protein-disulfide reductase (1.8.1.8), i.e. thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y108/00—Oxidoreductases acting on sulfur groups as donors (1.8)
    • C12Y108/04—Oxidoreductases acting on sulfur groups as donors (1.8) with a disulfide as acceptor (1.8.4)
    • C12Y108/04002—Protein-disulfide reductase (glutathione) (1.8.4.2), i.e. BdbC or BdbD
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/04—Intramolecular oxidoreductases (5.3) transposing S-S bonds (5.3.4)
    • C12Y503/04001—Protein disulfide-isomerase (5.3.4.1), i.e. disufide bond-forming enzyme
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/86—Products or compounds obtained by genetic engineering
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Birds (AREA)
  • Polymers & Plastics (AREA)
  • Nutrition Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Cosmetics (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention relates to a method of producing a protein disulfide redox agent, comprising i) cloning a DNA sequence coding for said protein disulfide redox agent from a donor cell, ii) making a DNA construct wherein said DNA sequence is under control of regulatory elements, iii) introducing said DNA construct into a host cell, iv) growing said host cell under conditions conductive to the production of the protein disulfide redox agent, and v) recovering and purifying said protein disulfide redox agent. Furthermore compositions comprising (i) a protein disulfide redox agent in combination with (ii) at least one redox partner, and optionally (iii) at least one or more other enzymes are demonstrated. The compositions can be used for the treatment or degradation of scleroproteins, especially hair, skin and wool, treatment and cleaning of fabrics, as additives to detergents, thickening and gelation of food and fodder, and pharmaceuticals for the alleviation of eye sufferings.
PCT/DK1994/000264 1993-06-28 1994-06-28 A method of producing a protein disulfide redox agent WO1995001420A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP94919568A EP0710281A1 (en) 1993-06-28 1994-06-28 A method of producing a protein disulfide redox agent
JP7503223A JPH08511944A (en) 1993-06-28 1994-06-28 Method for producing protein disulfide redox agent
AU70682/94A AU7068294A (en) 1993-06-28 1994-06-28 A method of producing a protein disulfide redox agent

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK0767/93 1993-06-28
DK93767A DK76793D0 (en) 1993-06-28 1993-06-28

Publications (2)

Publication Number Publication Date
WO1995001420A2 WO1995001420A2 (en) 1995-01-12
WO1995001420A3 true WO1995001420A3 (en) 1995-02-23

Family

ID=8097326

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/DK1994/000265 WO1995001425A1 (en) 1993-06-28 1994-06-28 Compositions comprising protein disulfide redox agents
PCT/DK1994/000264 WO1995001420A2 (en) 1993-06-28 1994-06-28 A method of producing a protein disulfide redox agent

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/DK1994/000265 WO1995001425A1 (en) 1993-06-28 1994-06-28 Compositions comprising protein disulfide redox agents

Country Status (5)

Country Link
EP (1) EP0710281A1 (en)
JP (1) JPH08511944A (en)
AU (2) AU7068294A (en)
DK (1) DK76793D0 (en)
WO (2) WO1995001425A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1171135A (en) * 1994-12-21 1998-01-21 诺沃挪第克公司 Method for dehairing of hides by enzymes
AU4538000A (en) * 1999-05-17 2000-12-05 Novozymes A/S Polypeptides with protein disulfide reducing properties
US7009087B1 (en) 2000-12-01 2006-03-07 Pioneer Hi-Bred International, Inc. Compositions and methods for altering the disulfide status of proteins
DE10059748A1 (en) * 2000-12-01 2002-06-20 Henkel Kgaa Enzymatic fixation of derivatized carbohydrates to fibrous materials
US20040203132A1 (en) 2003-02-28 2004-10-14 Cognetix, Inc. Conus protein disulfide isomerase
WO2016175577A1 (en) * 2015-04-30 2016-11-03 조선대학교 산학협력단 Perm oxidizing agent composition containing enzyme
CN114763552B (en) * 2021-01-12 2024-04-30 山东大学 Recombinant production method of microbial transglutaminase

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0272781A1 (en) * 1986-11-04 1988-06-29 The University Of Reading Treatment of hair
EP0277563A1 (en) * 1987-01-28 1988-08-10 Takeda Chemical Industries, Ltd. Polypeptide and production thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2141764A1 (en) * 1971-08-20 1973-03-01 Henkel & Cie Gmbh Scleroprotein - (esp hair-) reforming agent - contg protein disulphide reductase
DE2141763A1 (en) * 1971-08-20 1973-03-01 Henkel & Cie Gmbh Scleroprotein- (esp hair-) reforming agent - contg protein disulphide trans hydrogenase

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0272781A1 (en) * 1986-11-04 1988-06-29 The University Of Reading Treatment of hair
EP0277563A1 (en) * 1987-01-28 1988-08-10 Takeda Chemical Industries, Ltd. Polypeptide and production thereof

Also Published As

Publication number Publication date
DK76793D0 (en) 1993-06-28
WO1995001425A1 (en) 1995-01-12
AU7068394A (en) 1995-01-24
AU7068294A (en) 1995-01-24
WO1995001420A2 (en) 1995-01-12
JPH08511944A (en) 1996-12-17
EP0710281A1 (en) 1996-05-08

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