WO1994022289A1 - Procede pour obtenir des tomates presentant des caracteristiques de gout ameliorees et produit obtenu par ce procede - Google Patents

Procede pour obtenir des tomates presentant des caracteristiques de gout ameliorees et produit obtenu par ce procede Download PDF

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Publication number
WO1994022289A1
WO1994022289A1 PCT/US1994/003522 US9403522W WO9422289A1 WO 1994022289 A1 WO1994022289 A1 WO 1994022289A1 US 9403522 W US9403522 W US 9403522W WO 9422289 A1 WO9422289 A1 WO 9422289A1
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WO
WIPO (PCT)
Prior art keywords
plants
tomato
fructose
seeds
plant
Prior art date
Application number
PCT/US1994/003522
Other languages
English (en)
Inventor
Arthur Schaffer
Original Assignee
Peri Development Applications (1985) Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Peri Development Applications (1985) Ltd. filed Critical Peri Development Applications (1985) Ltd.
Priority to US08/530,216 priority Critical patent/US5817913A/en
Priority to AU64962/94A priority patent/AU6496294A/en
Publication of WO1994022289A1 publication Critical patent/WO1994022289A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A01H1/021—Methods of breeding using interspecific crosses, i.e. interspecies crosses
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/08—Fruits
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/82—Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
    • A01H6/825—Solanum lycopersicum [tomato]

Definitions

  • the present invention relates to a method of breeding tomatoes having superior taste characteristics and to tomatoes having superior taste characteristics and to products of the method.
  • Tomato Fruit Quality Components Plant Breeding Reviews, 4, 274-310) .
  • One of the major components of taste in tomatoes is soluble sugar content.
  • the soluble sugar content of all known commercial cultivars of tomato primarily includes the hexose sugars glucose and fructose in ratios of approximately 1:1 to 1:1.5 (Davies, J.N and Hobson, G.E., 1981, The Constituents of Tomato Fruit - The Influence of the Environment. Nutrition and Genotype, CRC Critical Reviews in Food Science and Nutrition, 15: 205 - 280; Davies, J.N. and Kempton, R.J., 1975, Changes in the Individual Sugars of Tomato Fruit During
  • TSS total soluble solids
  • the present invention seeks to provide an improved method for breeding tomato plants having superior taste characteristics and products of the method.
  • a method for breeding tomato plants that produce tomatoes having superior taste characteristics including the steps of, crossing at least one Lycopersicon esculentum plant with a Lycopersicon spp. to produce hybrid seeds, collecting the hybrid (F_) seeds, growing plants from the F. seeds, pollinating the F. plants, collecting the hybrid seeds produced by the F. plants, growing plants from the seeds produced by the F. plants, measuring sucrose, glucose and fructose content of ripe fruit produced from the plants grown from the seeds of the F_ plants; and selecting plants with tomato fruits having desired characteristics including a fructose/glucose ratio greater than 1.8 and fructose levels higher than 1.3% on a fresh weight basis.
  • the method for breeding tomato plants additionally includes the steps of crossing plants which have been selected according to the method of claim 1 with a Lycopersicon plant and selecting plants with tomato fruits having desired characteristics including a fructose/glucose ratio greater than 1.8 and fructose levels higher than 1.3% on a fresh weight basis.
  • the steps of crossing and selecting are repeated at least once.
  • the method for breeding tomato plants additionally includes the steps of selfing, at least once, the plants, and selecting plants with tomato fruits having desired characteristics including a fructose/glucose ratio greater than 1.8 and fructose levels higher than 1.3% on a fresh weight basis.
  • the Lycopersicon spp. plant having a fructose: glucose ratio greater than 1.8 is a Lycopersicon hirsutum plant.
  • crossing includes sexual crossing.
  • crossing includes asexual crossing.
  • asexual crossing includes somatic cell hybridization.
  • the step of pollinating includes self pollination.
  • the step of pollination includes back crossing with a Lycopersicon esculentum plant.
  • the method for breeding tomato plants additionally includes the step of propagating the plants with tomato fruits having the desired characteristics.
  • the step of propagating includes the step of vegetative propagation.
  • the step of propagating includes the step of propagation by seed.
  • a tomato plant produced according to the method described hereinabove In accordance with yet a further preferred embodiment of the present invention there is provided a tomato fruit produced by a tomato plant produced according to the method described hereinabove. In accordance with another preferred embodiment of the present invention there is provided tomato seeds which when grown yield a tomato plant produced according to the method described hereinabove. There is also provided in accordance with the present invention a heterozygous tomato plant producing fruit having a fructose/glucose ratio greater than 1.8 and fructose levels higher than 1.3% on a fresh weight basis. In accordance with a preferred embodiment of the present invention the tomato plant includes tomato fruit.
  • the tomato plant includes tomato seeds which when grown yield the tomato plant described hereinabove.
  • the elevated fructose/glucose ratio providing a sweeter taste than that found in tomatoes having a similar amount of total sugars but with a normal fructose to glucose ratio.
  • the method for breeding tomato plants includes first hybridizing at least one Lycopersicon esculentum plant with a Lycopersicon hirsutum plant. The fruits of the L esculentum plants are then allowed to ripen and the hybrid (F ⁇ ) seeds are collected.
  • F--_ seeds are then planted. F ⁇ plants are grown and then allowed to self pollinate. The self pollinated flowers are then allowed to produce ripe fruits and the F 2 seeds are collected. These seeds are then planted. Plants grown from these seeds are allowed to produce fruits which are harvested.
  • the harvested fruits are then analyzed for °BRIX, sucrose, glucose and fructose content, using methods described below, and plants with tomato fruits having desired characteristics including a fructose/glucose ratio greater than 1.8 and fructose levels higher than 1.3% on a fresh weight basis are selected.
  • the selected plants may then be propagated for use by vegetative propagation methods such as micropropagation or by sexual propagation methods.
  • the selected plants may also be selfed for at least one generation or crossed with other L esculentum cultivars to create varieties which incorporate characteris ⁇ tics other than the high fructose/glucose ratios selected for.
  • These derived plants may then be propagated either vegetatively or by seed based propagation.
  • L esculentum breeding line 1630 (a Volcani Institute male sterile breeding line) were crossed with the wild species L ⁇ . hirsutum (LA 1777) using the L_j_ esculentum breeding line as the female parent.
  • the L_-- esculentum parent contained approximately equi olar concentrations of glucose and fructose (see Table 1) .
  • the L_j_ hirsutum parent (LA 1777) accumulated sucrose but had a low concentration of fructose/glucose and a fructose glucose ratio of 2.3 but with less than 1.3% fructose on a fresh weight basis (see Table 1) .
  • the fruits of the L esculentum plants were then allowed to ripen and the hybrid (F- ⁇ ) seeds collected. Hybrid F ⁇ seed was sown and plants grown. The plants were allowed to self pollinate and after the ripening of the fruit F 2 seed was collected.
  • the F 2 seeds were sown and about 350 F 2 plants were grown and allowed to self pollinate.
  • the fruits were allowed to ripen and fruit from each individual plant which produced fruit was harvested and individually analyzed for TSS (°BRIX), and soluble sugar content, as described hereinbelow.
  • HPLC High Pressure Liquid Chromotography
  • sucrose, glucose and fructose values of fruit of selected F 3 plants are shown in Table 1.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Physiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Animal Husbandry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Le procédé pour obtenir des plants de tomate donnant des tomates ayant des caractéristiques améliorées de goût comprend les étapes consistant à croiser au moins un plant de Lycopersicon esculentum avec un plant de Lycopersicon spp. pour produire des graines hybrides, à recueillir les graines hybrides (F1), à obtenir des plants à partir des graines F1, à assurer la pollinisation des plants F1, à recueillir les graines hybrides produites par les plants F1, à obtenir des plants à partir des graines produites par les plants F1, et à mesurer la teneur en saccharose, glucose et fructose des tomates mûres produites par des plants obtenus à partir des graines des plants F1. On sélectionne alors les plants produisant les tomates ayant les caractéristiques souhaitées, en particulier un rapport fructose/glucose supérieur à 1,8 et une teneur en fructose supérieure à 1,3 % en poids par rapport à la tomate fraîche.
PCT/US1994/003522 1993-03-31 1994-03-30 Procede pour obtenir des tomates presentant des caracteristiques de gout ameliorees et produit obtenu par ce procede WO1994022289A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US08/530,216 US5817913A (en) 1993-03-31 1994-03-30 Method for breeding tomatoes with superior taste characteristics and product of the method
AU64962/94A AU6496294A (en) 1993-03-31 1994-03-30 Method for breeding tomatoes with superior taste characteristics and product of the method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IL105,243 1993-03-31
IL10524393A IL105243A (en) 1993-03-31 1993-03-31 Method for breeding tomatoes with superior taste characteristics and product of the method

Publications (1)

Publication Number Publication Date
WO1994022289A1 true WO1994022289A1 (fr) 1994-10-13

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Country Status (3)

Country Link
AU (1) AU6496294A (fr)
IL (1) IL105243A (fr)
WO (1) WO1994022289A1 (fr)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999004621A1 (fr) 1997-07-23 1999-02-04 State Of Israel - Ministry Of Agriculture Marqueur moleculaire pour le gene determinant le rapport fructose/glucose dans une tomate mure
WO2000005390A3 (fr) * 1998-07-20 2000-08-24 Israel State Procede relatif au controle de la synthese de l'amidon
EP1691600A2 (fr) * 2003-11-06 2006-08-23 Arcadia Biosciences, Inc. Tomates a activite invertase acide alteree par suite d'alterations non transgeniques dans les genes d'invertase acide
WO2012170768A3 (fr) * 2011-06-10 2013-05-16 University Of Florida Research Foundation, Inc. Tomates hybrides et procédés de préparation de tomates hybrides
WO2013163272A1 (fr) * 2012-04-24 2013-10-31 University Of Florida Research Foundation, Inc. Compositions et procédés permettant de modifier la perception de la sucrosité
US9501699B2 (en) 2011-05-11 2016-11-22 University Of Florida Research Foundation, Inc. Systems and methods for estimating the geographic location at which image data was captured

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4940839A (en) * 1986-10-01 1990-07-10 Dna Plant Technology Corporation Method of tomato protoplast fusion and regeneration of hybrid plants therefrom

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4940839A (en) * 1986-10-01 1990-07-10 Dna Plant Technology Corporation Method of tomato protoplast fusion and regeneration of hybrid plants therefrom

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
HORTSCIENCE, Volume 23, No. 3, issued June 1988, A.C. MEYER et al., "Estimating Heritability and the Number of Genes Involved in Controlling Fructose and Glucose Concentrations in Tomato (Lycopersicon Esculentum)", page 767, abstract no. 346. *
NATURE, Volume 209, issued 05 February 1966, J.N. DAVIES et al., "Occurence of Sucrose in the Fruit of Some Species of Lycopersicon", pages 640-641. *
PLANT PHYSIOLOGY, Volume 95, No. 2, issued February 1991, D. MIRON et al., "Sucrose Phosphate Synthase, Sucrose Synthase, and Invertase Activities in Developing Fruit of Lycopersicon Esculentum Mill, and the Sucrose Accumulating Lycopersicon Hirsutum Humb. and Bonpl.", pages 623-627. *
PLANT PHYSIOLOGY, Volume 95, No. 4, issued April 1991, S. YELLE et al., "Sink Metabolism in Tomato Fruit. IV. Genetic and Biochemical Analysis of Sucrose Accumulation", pages 1026-1035. *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999004621A1 (fr) 1997-07-23 1999-02-04 State Of Israel - Ministry Of Agriculture Marqueur moleculaire pour le gene determinant le rapport fructose/glucose dans une tomate mure
WO2000005390A3 (fr) * 1998-07-20 2000-08-24 Israel State Procede relatif au controle de la synthese de l'amidon
US6720485B1 (en) 1998-07-20 2004-04-13 State Of Israel-Ministry Of Agriculture Controlling starch synthesis
EP2138033A1 (fr) * 1998-07-20 2009-12-30 The State Of Israel-Ministry Of Agriculture Procédé relatif au contrôle de la synthèse de l'amidon
EP1691600A2 (fr) * 2003-11-06 2006-08-23 Arcadia Biosciences, Inc. Tomates a activite invertase acide alteree par suite d'alterations non transgeniques dans les genes d'invertase acide
EP1691600A4 (fr) * 2003-11-06 2007-09-05 Arcadia Biosciences Inc Tomates a activite invertase acide alteree par suite d'alterations non transgeniques dans les genes d'invertase acide
US9501699B2 (en) 2011-05-11 2016-11-22 University Of Florida Research Foundation, Inc. Systems and methods for estimating the geographic location at which image data was captured
WO2012170768A3 (fr) * 2011-06-10 2013-05-16 University Of Florida Research Foundation, Inc. Tomates hybrides et procédés de préparation de tomates hybrides
WO2013163272A1 (fr) * 2012-04-24 2013-10-31 University Of Florida Research Foundation, Inc. Compositions et procédés permettant de modifier la perception de la sucrosité

Also Published As

Publication number Publication date
AU6496294A (en) 1994-10-24
IL105243A0 (en) 1993-07-08
IL105243A (en) 2001-08-26

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