WO1994016075A3 - Process for producing m-csf 223 - Google Patents

Process for producing m-csf 223 Download PDF

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Publication number
WO1994016075A3
WO1994016075A3 PCT/US1994/000658 US9400658W WO9416075A3 WO 1994016075 A3 WO1994016075 A3 WO 1994016075A3 US 9400658 W US9400658 W US 9400658W WO 9416075 A3 WO9416075 A3 WO 9416075A3
Authority
WO
WIPO (PCT)
Prior art keywords
csf
amino acid
wild
type
polypeptide
Prior art date
Application number
PCT/US1994/000658
Other languages
French (fr)
Other versions
WO1994016075A2 (en
Inventor
Andrew Dorner
Edward F Fritsch
Robert Steininger
Lawrence Bush
Original Assignee
Genetics Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetics Inst filed Critical Genetics Inst
Priority to JP6516370A priority Critical patent/JPH08505534A/en
Priority to AU61252/94A priority patent/AU6125294A/en
Publication of WO1994016075A2 publication Critical patent/WO1994016075A2/en
Publication of WO1994016075A3 publication Critical patent/WO1994016075A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

DNA molecules which encode a 255 amino acid polypeptide, comprising the 32 amino acid M-CSF leader sequence and the 223 amino acid mature M-CSF polypeptide, and lack the coding sequence for the carboxy-terminal propeptide sequence present in wild-type M-CSF DNA, result in highly efficient production of M-CSF, which displays similar post-translational modifications as wild-type M-CSF and is unaffected in biological properties.
PCT/US1994/000658 1993-01-13 1994-01-12 Process for producing m-csf 223 WO1994016075A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP6516370A JPH08505534A (en) 1993-01-13 1994-01-12 Method for manufacturing M-CSF223
AU61252/94A AU6125294A (en) 1993-01-13 1994-01-12 Process for producing m-csf 223

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US414193A 1993-01-13 1993-01-13
US08/004,141 1993-01-13

Publications (2)

Publication Number Publication Date
WO1994016075A2 WO1994016075A2 (en) 1994-07-21
WO1994016075A3 true WO1994016075A3 (en) 1994-12-22

Family

ID=21709373

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1994/000658 WO1994016075A2 (en) 1993-01-13 1994-01-12 Process for producing m-csf 223

Country Status (3)

Country Link
JP (1) JPH08505534A (en)
AU (1) AU6125294A (en)
WO (1) WO1994016075A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2746412B1 (en) * 1996-03-21 1998-06-12 Rhone Poulenc Rorer Sa PURIFICATION OF PLASMID DNA OF PHARMACEUTICAL QUALITY
US6136536A (en) * 1997-10-29 2000-10-24 Genetics Institute, Inc. Rapid generation of stable mammalian cell lines producing high levels of recombinant proteins
EP1721986A1 (en) * 1997-10-29 2006-11-15 Genetics Institute, LLC Rapid generation of stable mammalian cell lines producing high levels of recombinant proteins

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987006954A1 (en) * 1986-05-06 1987-11-19 Genetics Institute, Inc. Production of m-csf
EP0261592A1 (en) * 1986-09-17 1988-03-30 Otsuka Pharmaceutical Co., Ltd. Gene coding for human colony-stimulating factors
WO1988003173A2 (en) * 1986-10-24 1988-05-05 Cetus Corporation New forms of colony stimulating factor-1
US4868119A (en) * 1986-12-11 1989-09-19 Genetics Institute, Inc. Hematopoietic growth factors
US4879227A (en) * 1986-05-06 1989-11-07 Genetics Institute, Inc. Production of a recombinant human colony stimulating factor
FR2667612A1 (en) * 1990-10-09 1992-04-10 Toyo Jozo Kk STABLE PRODUCTION OF M-CSF ACTIVE POLYPEPTIDE

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987006954A1 (en) * 1986-05-06 1987-11-19 Genetics Institute, Inc. Production of m-csf
US4879227A (en) * 1986-05-06 1989-11-07 Genetics Institute, Inc. Production of a recombinant human colony stimulating factor
EP0261592A1 (en) * 1986-09-17 1988-03-30 Otsuka Pharmaceutical Co., Ltd. Gene coding for human colony-stimulating factors
WO1988003173A2 (en) * 1986-10-24 1988-05-05 Cetus Corporation New forms of colony stimulating factor-1
US4868119A (en) * 1986-12-11 1989-09-19 Genetics Institute, Inc. Hematopoietic growth factors
FR2667612A1 (en) * 1990-10-09 1992-04-10 Toyo Jozo Kk STABLE PRODUCTION OF M-CSF ACTIVE POLYPEPTIDE
GB2249100A (en) * 1990-10-09 1992-04-29 Toyo Jozo Kk Expression of M-CSF deletion mutant polypeptides

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MASAYUKI TAKAHASHI ET AL: "Amino-terminal region of human macrophage colony-stimulating factor (M-CSF) is sufficient for its in vitro biological activity : Molecular clonig and expression of carboxyl-terminal deletion mutants of human M-CSF", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 161, no. 2, 15 June 1989 (1989-06-15), DULUTH, MINNESOTA US, pages 892 - 900 *
S.C. CLARK ET AL: "The human hematopoietic colony-stimulating-factors", SCIENCE, vol. 236, June 1987 (1987-06-01), LANCASTER, PA US, pages 12229 - 1237 *

Also Published As

Publication number Publication date
WO1994016075A2 (en) 1994-07-21
AU6125294A (en) 1994-08-15
JPH08505534A (en) 1996-06-18

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