WO1994015632A1

WO1994015632A1 - Vaccine against trichophytosis in animals

Info

Publication number: WO1994015632A1
Application number: PCT/RU1993/000319
Authority: WO
Inventors: Ljubov Markovna Yablochnik; Karen Artemovich Sarkisov; Konstantin Pavlovich Letyagin; Alexandr Nikolaevich Panin
Original assignee: Vserossiisky Gosudarstvenny Nauchno-Issledovatelsky Institut Kontrolya, Standartizatsii I Sertifikatsii Veterinarnykh Preparatov (Vghki)
Priority date: 1992-12-30
Filing date: 1993-12-27
Publication date: 1994-07-21
Also published as: AU5824594A; RU2018321C1

Abstract

The invention concerns the field of veterinay science, in particular the prevention and treatment of trichophytosis in animals. The proposed trichophytosis vaccine differs from known vaccines in containing in addition an antigen of the strain VGNKI No. 27 of the fungus Trychophyton mentagrophytes in quantities sufficient to provoke an immune reaction in an animal.

Description

PROPERTY FOOD ΤΡIΧ05IΤII

BACKGROUND OF THE INVENTION 5 The invention is not available in biotechnology, particularly in the presence of vascular vaccines.

BACKGROUND OF THE INVENTION

Western vaccine LZZ-ZZN n vaccine ΤΦ-ΙЗΟΚ / prativiv Ι With the traffic of animals

Lizata (cϊ / .. Patent of Zelibro 1330240, cl. Α53, 15 1973; Automatic testimony of CCC. "- 955571, cl. ΑбΙΚ 39/00, 1980).

Unfortunate:.: There are known to be ill, that they only neglect the illness at the other end of the hospital,

3 correspondences with _ΟΟS-UΙ (1902) (see Z.Y. Letsenko, "Disease diagnosis of pungent diseases", Ιά.,: Ma

25 τ.κ. according to the class of Georgy (1957) (group .. also, p. 34), the causative agent is triplet and :: its basic name is .

The purpose of the invention is to operate on a fully-functional basis, from which 30 .7. ν <-τ ^} _Λлλ.ζ _£ >_> иνи_. _ι 7ъ νζllsh_ £> _- yl-

_χ ϊУ? 1: χ уρ

Factory Access

The stated goal is reached .. that the vaccine is available - 35

0 _ < _/ ο /. - 2 -

Игntigen from the strain УУγ νе ΖΖи.С_θ £ ___- /? G- 130 L 300- ^β ΟΟ million m

0 cm3

ΑнτπгΙΪ шЗ Штамυа

I. mlk shkροκοni-

Sugar 10, 0-20, 0

10 gel 1, 5-4, 0

The rest of the world

DETAILED DESCRIPTION OF THE INVENTION

fifteen

eshosticheskogo isolated from the white mouse, the methods are on the safe side of the mainstream of the market for the most rapid loss of business is faster

20

lectures of microorganisms of the Russian Federation

25 амτammb L. L.

ΒГΗШΙ. ^! 327 are used as follows and are protected.

Ινϊορφologic statement Yıshcheli κ ο ρ ρ аш аш,,,, ο ο ο ο, аш,, κ аш κ, Ы Ы Ы Ы Ы Ы Ы Ы Ы Ы Ы Ы.

30 percent), the diameter of the mycelium is 2.0-3.0 μm. Ι.Ιπκροκοдд м мππππππчччччччччччччч,,,,,,,,,,,,,,,,,, угуглыелые,, г г г гушушушевушуш, ненеев, уд, уд, ,нене ,ненели бл бл бл бл бл бл:::::::: :ал :алалалалалалалалалалϋ 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 цел 1,5 1,5 1,5 1,5 1,5 1,5 цел цел цел цел 1,5 цел цел him, it is more common, ΙΙаι: ρο- κοnidii are single. Personally Transmitted: 1-5, Chatsu 1-2,

35 ρmeasure οου I0-30x3-8 mkm.

From 20-30 days of age, you can observe the sweeteners of the environment ^, with two-sided, always - 3 -

single, 3.0-5.0 gk; in the diagram. Occupants are aware of the private parts of the city, the walls are private, for example,

5 gynoetalnaya vylp lazyzkozdnye gikrupokonpdpn 3.0-9.0 gk Cultural complications. In the case of bustle-agar, it grows in the video area of the territory of Belarus, the profitability of the community is insignificant;.; Reduced loss of life, loss of health, loss of health, loss of health 50–9C g ... (15-20 days old

Yu τуρа).

On the Sabareu agrast, it grows in the form of a flat, white, velvet with a quick release:.; well-known. increased in the center, growing at the expense of the gry;; a reversal of the pale. kelty, sometimes more than the average: the center, the rate of 50-90

1 (15-20 days old culture).

In the midst of Gennebug, it will run out of front of Belon, baruchatstst, radial fold: the kolonpπ, the growing krpund, the result is a payload, 0 ποροοbρazovanpe. The reduction in output from the wort-agar-z is 198.4-259.2 hl / sZ.

SURPRISE RESPONSE. Ρ ыш з ыш ыш ыш .: .: .:.

At 27 ° C, in pigs, κ свин π,,,,, зе зе υ κ,, еля, calves down after 2-3 weeks for 5 gestational injections инъ з з з ва ва...... They are localized: a good result, but 2.0-2.5 s, but a saga - will be neglected for 15–20 days.

nothing to skarpig-- 0 cited to a dose of 0.25 ... liι / cg of 3 sockets in the country, the appendix is open for 10-20 days; source Ideal for the mercantile state; πό communication. Occupation: It is inactive.

Antigeneration. ηa zvezdnizε cultivars of step 5 of the city. Therefore, there is no need to forget about agglutination in the air from 1: 40-1: Ι2ЗЗ. - 4 -

Lasting Interest. In case of zukrat:.; internally:.; retraction of the tel.:., circle:.: vaccines, vaccine; from the data from _ltaia ;,: and in the dose, which is 10-20 it-ι shyzyn yykροκοπ-

-din, living things happen: Kenny and:.;:, it’s worse for the next

Well, there is no v.: it is more than δ leth (observation period).

Shtayl Ιh ..

ZGSHI I ^th 27 οτlichaeτsya οτ 10 eπizοοτichesκin slabοG: viρulenτnοsτyu, οτsuτsτviey zaρa: κe- nπya πρπ κοnτaκτe b; e; κdu zhizοτnyyπ, vysοκο :: aκτivnοsτyu HA κοπleπiya yiκροκοnπdy and iyyunοgennοsτyu πρπ znuτρi: .. yashech- nοϊ; introduction of livestock.

15 Other Vaccines Received from the Invention ПЖЖ? I.

20 agarе (ρΗ 6,2-6,0) πρ and τеι.more than 2b-28οС for 12-20 days.

A swollen stump is located in the back; Cultures under conditions of accep- ticism reduce the benefits of a healthy environment: special: and they are stored in stylish banks. Flexible yassu

25 Each of the United States is goryuniziruyut zypkseran or kolloidnopi v / eln; vce, and then: - .; with πο:.; in general, the stylish rooms have been upgraded: Г ο ο ο ((((<<<<<<<(<_ е (- - <. γ. 130 130 130 ус).

ЗГΗΚП "^'' 27 - 600 YLΗ / SU.Z. Jesus Christ-

30 Penetration is due to the absence of penicillin or :: constituent acid, at a rate of 100 Ε D for I second;.: 3 gene .-. G:: Ogenet shtayyya musha 1g. νel νи - ^ - αт. 130 Lives a variety of things :; Communion with the Great Sha'zh group

previous :. It can be easily removed, and it can be expanded with a solid α. It is not limited to dry. 5 -

ПΡПΙлΞΡ 2.

The vaccine is being produced similarly, in general, I have used it to use it :. Generation of the State:.;:.:

As a result of the stabilization of the original product, the result is 15 sugar candies, 5 γ of the parent.

.υ

PΡΙΙΙ.ΕΡ 3.

The bacterium is produced by living in a similar fashion, as it is in I. For its use, it is good to eat the gene. ν -ъναι <* lll 5,130 L with an incentive ι..κκροκοnхдкк 600;,: ln / s.; 3

desirable.

20 Gοτοvaya vaκtsκna πρedsτazlyaeτ sοbο ^": οdnοροdnuyu πορis- τuyu suχu-e yassu in vκde τableτκκ seροgο tsveτa Βaκtsκna sοχρanyaeτ svοi biοlοgichesκκe svοκsτva πρi uslοziπ χρane- Niya in zaκρyτyχ suχκχ, τeynyχ ποιleschenπyaχ πρκ τeyπeρaτuρe 2-Ι0 ° C Sροκ gοdnοsτκ 12 months... Κ vaccine is being tested

2 vials of solid diluent; ..

PI 4.

The resulting selections are va- rious due to live traffic::: 1, 2, 3 before transfer: 'I dispose of it 30 times. This is the case for the series: which is available on an actual basis after the following evaluation of the general antigenesis in the textbook (Table I).

ΤΑΕLΙSCHΑ I

Зδ

Filing the antigen Zaktsna ο ци ци ци ци За За За За За За За κ κ κ κ κ κ κ κ κ κο За κ ο κ ο ο κ ο ο ο ο ο κ ο

7g νζlml _θ τ w 'τη, 130 L 5 7, 5 ΙС - 6 -

Table I

ΪГ »27 ЗПΙΚИ 10 7.5 5

Actually, for examples 1, 2, 3, they are used for communalization of calves by introducing internal muscle from the interer ”: 10-

10 days in the dose of I cm3.

3 In the short run, you will experience a similar production of vases from the factory. νе лиβ & _м, un 130 L and 1 h.

ΒGΗΚI G ^C 27 with a concentration of mycopindia antigen Ε a natural dose of 7.5 mln / cm3.

15 After 35 days after the second lunarization, they incarcerate virulent and cultured states 7g_, ν- & ^/ ζΛ ес ^, ≤ *. ?! '- 153

0 they infect the same culture. And they are in contact with (non-immunizing baths) calves.

The results of the experiment. oral infection calves Delivery: in table 2. 5 ΤΑΕ 5ΙЦΑ 2

]. *. "Κ-vο Biοπροπeρaτ Ishu- Κοlκcheτsτvο zabοlezshiχ gρ. Zhizοτnyχ isποlzuemy niziρ. Zhizοτnyχ πρi eκsπeρi- Ε gρuπ- for imιuniza- τelyaτ .en alnο _z_ and en ^ _ :: _ πe tsκi τelyaτ (gοl.) _Τz _ _that _ _τс _ _Τ З - ΤЗ-

153 251 724 118 20 0

C 0 0 υ

0 s 0 s 5 Table 2

3 7 Zaktsina πο πρиϊлеρу 3 7 0 С 0 0 υ

4 10 Standard vaccine 10 0 4 2 0 .ννν сοζиνη

ΙЗΟЛ

10 5 Zaczin from the staff of v. Vla 9 δ 0 I 2 <- »

Α.

ЗГΗЮΪ I? 27 b b

(neim.unizi- 1 ροvannye) - 2

From the table it is visible that 1.2 and 3 series of vapin y ;;:.:; Imm

20 nizatsya calves ϊν_onovacinia_: and does not cause a sufficiently long-term and viva.uniteta for the next. /. contagious heterogeneous cultures of pathogens are toxic. I didn’t have κι.α.- lower ones ı not worse then:;; live ı EXCEPT:.: Ntal: su за zirulent ow;

25 telephones.

FUN 5.

Τ е ля ΟΚ ΤΗ ΤΗ ΤΗ Ι Ι Ι ... And the series:.:: Vakt ва: akalogogich πρπ:,: ery 4 and;. ;;.:: Κ:: ο 14 с с с с с с с .. .. .. .. .. .. .. .. .. .. .. .. ..; virulent- 30 b. g l * -υΟю ^< Я.уν_. - 153 κ 1 251. Economic Railways (2

All the vascularized infections did not get sick, all the signs of the disease were removed from the terminal with 35 selected cases from the excluded cases. - ε -

ΠΡΚ .ΕΡ 6.

For Ii Guniz πππ 10 κ

5 v.-assy 2, 5-3, 0 kg use the option πο: ι: ery: 2. Most importantly π г г ас ас / / /;

The following accent is received: а Actighez I with a physical dose: Ya. Vchtgηl_. &&<-. ^γ _. 130 L 7, 5: LLH

YU

ΒГΗΚΙΙ? * 27 7, 5 ι: лκ. ілπκροκο кидии. Zakutsu is well-known for his acquaintances:.: Muscular in the home

Most of the time, it’s possible from station 7. \ uh £ ~ su_νρ \ £ ^ ^r 1__-

15: ^* 135, content on the first day at 20

Practically in I since:: 3, they take a look at the inside of the muscle in the area of the mute. After 30 days, it’s easy to get ready for work. t ^ ^ οψ and ^ Ιг ^ - 251 and 1b, 25 0

ο isποlzοvanπe zaκtsiny sποsοbsτzueτ οbρazοzaniyu nadezhnοgο iπuniτeτa in vseχ iιlιuniziροzannyχ κροliκοz in το vρeι I κaκ isποlzοzanie izzesτnοy vaκtsnκy Μenτazaκ not daeτ Nadia zhnοgο eφφeκτa - 2 κροlnκa 10 zabοleli τριπsοφiτπeG: and;? 5 foci of success; the culture is highlighted in the incarnation.

ПΡИ ÷ .ΞΡ 7.

20 τelyaτ ιπlgunizπρuyuτ zaρπanτaππ vaκtsπny πο πρinegu I and 3 κοκπeκτρaπieπ aκτπgεnοz in πρπznzοchnοg dzzz πο ^~: n 0 geρu 4 Κa.κdο "gρuππe τelyaτ (10 gοlο) zaκπnnu πρi enyayuτ BΗ- :: '. The last year in the first day, I had to wait 10-14 days: After 4 and 9 days after that ^{, the} second time, the group would be ignored.

251. 5 3 As a result, no red tape did not become ill. Downloading this product is protected by the inadvertent loss of γ; it disappears after 4 π 9; one more time after the fact. <__>

The vaccine is highly effective by treating the patient, the vaccine is: the goal is: it removes two

5 months. 1 10-14 days in the following doses: calves from 14 days: up to 4 months I cm3, older than 5 ^* months 2 s.: 3 and small prices (October, 6 days old) , 5 cm3, older than the second - цев cm3. For masters with a weight of 2.5-3.0 kg, 0.3-0.5 si / 3 is introduced with a regular dose

10 ly. 'With a curative purpose, the vaccine was taken up by a healthy one, which was short of an interval.' 10-14 days in an double dose.

The use of the vaccine in the invention in the practice of the disease will improve the efficiency of the treatment of the risk of vomiting.

fifteen

20

25

thirty

35

Claims

- 10 -

ΦΟΡΙ.ΙУЛΑ ΙΪЗΟΕΡΞΤΞΙШ

Zaktsina on the other hand, which includes the state law:. :: a

130 Sugar, kelatin and water, so that you can get additional antigen from the 1st stage.

ΒГΗΚП "- 27 πρπ as followsϊν: ο ο τ ен ен π π κ κ ((вес вес вес вес вес вес вес вес вес вес вес вес вес вес вес вес вес вес вес вес вес вес вес вес вес вес вес вес вес вес:::::::

130 L

300-600 School / Anthony Antigene from the United Kingdom.

ZGSHI? ^g 27

300-600 million micronidium /

sugar 10.0-20.0 gelatin 1.5-4.0 other water