WO1994010315A3 - Process for enhancing the content of a selected amino acid in a seed storage protein - Google Patents

Process for enhancing the content of a selected amino acid in a seed storage protein Download PDF

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Publication number
WO1994010315A3
WO1994010315A3 PCT/US1993/010090 US9310090W WO9410315A3 WO 1994010315 A3 WO1994010315 A3 WO 1994010315A3 US 9310090 W US9310090 W US 9310090W WO 9410315 A3 WO9410315 A3 WO 9410315A3
Authority
WO
WIPO (PCT)
Prior art keywords
seed storage
storage protein
amino acid
selected amino
enhancing
Prior art date
Application number
PCT/US1993/010090
Other languages
French (fr)
Other versions
WO1994010315A2 (en
Inventor
Barbara Ballo
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Priority to AU54466/94A priority Critical patent/AU5446694A/en
Publication of WO1994010315A2 publication Critical patent/WO1994010315A2/en
Publication of WO1994010315A3 publication Critical patent/WO1994010315A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Methods which allow for nutritional improvement of plants and plant tissue by increasing the amount of a selected amino acid(s) in a seed storage protein involve altering a naturally-occurring seed storage protein gene. Oligonucleotides coding for the protein are assembled by use of overlapping synthesized DNA sequences.
PCT/US1993/010090 1992-10-23 1993-10-22 Process for enhancing the content of a selected amino acid in a seed storage protein WO1994010315A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU54466/94A AU5446694A (en) 1992-10-23 1993-10-22 Process for enhancing the content of a selected amino acid in a seed storage protein

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US96566492A 1992-10-23 1992-10-23
US07/965,664 1992-10-23

Publications (2)

Publication Number Publication Date
WO1994010315A2 WO1994010315A2 (en) 1994-05-11
WO1994010315A3 true WO1994010315A3 (en) 1994-09-15

Family

ID=25510303

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1993/010090 WO1994010315A2 (en) 1992-10-23 1993-10-22 Process for enhancing the content of a selected amino acid in a seed storage protein

Country Status (2)

Country Link
AU (1) AU5446694A (en)
WO (1) WO1994010315A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69428290T2 (en) * 1993-01-13 2002-04-18 Pioneer Hi-Bred International, Inc. DERIVATIVES OF ALPHA-HORDOTHIONIN WITH HIGHER LEVELS OF LYSINE
US6326527B1 (en) * 1993-08-25 2001-12-04 Dekalb Genetics Corporation Method for altering the nutritional content of plant seed
CN1195377A (en) * 1995-05-31 1998-10-07 先锋高级育种国际公司 Methods of increasing accumulation of essential amino acids in seeds
MX9709352A (en) * 1995-06-02 1998-02-28 Pioneer Hi Bred Int HIGH METHIONINE DERIVATIVES OF 'alpha'-HORDOTHIONIN.
BR9609200A (en) * 1995-06-02 1999-05-11 Pioneer Hi Bred Int High threonine derivatives of alpha-hordothionine
US5850016A (en) * 1996-03-20 1998-12-15 Pioneer Hi-Bred International, Inc. Alteration of amino acid compositions in seeds
AU728086B2 (en) * 1996-11-01 2001-01-04 Pioneer Hi-Bred International, Inc. Proteins with enhanced levels of essential amino acids
US6800726B1 (en) 1996-11-01 2004-10-05 Pioneer Hi-Bred International, Inc. Proteins with increased levels of essential amino acids
ZA981569B (en) * 1997-04-08 1999-08-25 Du Pont An engineered seed protein having a higher percentage of essential amino acids.
US6197510B1 (en) * 1998-10-01 2001-03-06 Bio-Id Diagnostic Inc. Multi-loci genomic analysis
EP1180547A3 (en) * 2000-07-28 2004-01-28 Nisshinbo Industries, Inc. Method for producing DNA
US8785130B2 (en) 2005-07-07 2014-07-22 Bio-Id Diagnostic Inc. Use of markers including nucleotide sequence based codes to monitor methods of detection and identification of genetic material
US9150906B2 (en) 2006-06-28 2015-10-06 Bio-Id Diagnostic Inc. Determination of variants produced upon replication or transcription of nucleic acid sequences

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0208418A2 (en) * 1985-06-12 1987-01-14 Lubrizol Genetics Inc. Modified zein
EP0318341A1 (en) * 1987-10-20 1989-05-31 Plant Genetic Systems, N.V. A process for the production of transgenic plants with increased nutritional value via the expression of modified 2S storage albumins in said plants
WO1991004270A1 (en) * 1989-09-20 1991-04-04 Commw Scient Ind Res Org Modified seed storage proteins
WO1991013993A1 (en) * 1990-03-05 1991-09-19 The Upjohn Company Protein expression via seed specific regulatory sequences

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0208418A2 (en) * 1985-06-12 1987-01-14 Lubrizol Genetics Inc. Modified zein
EP0318341A1 (en) * 1987-10-20 1989-05-31 Plant Genetic Systems, N.V. A process for the production of transgenic plants with increased nutritional value via the expression of modified 2S storage albumins in said plants
WO1991004270A1 (en) * 1989-09-20 1991-04-04 Commw Scient Ind Res Org Modified seed storage proteins
WO1991013993A1 (en) * 1990-03-05 1991-09-19 The Upjohn Company Protein expression via seed specific regulatory sequences

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
SANDHU, G.S., ET AL.: "Dual asymmetric PCR: one-step construction of synthetic genes", BIOTECHNIQUES, vol. 12, no. 1, January 1992 (1992-01-01), pages 14 - 16 *
YE, Q-Z, ET AL.: "Gene synthesis and expression in E.coli for PUMP, a human matrix metalloproteinase", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 186, 15 July 1992 (1992-07-15), DULUTH, MINNESOTA US, pages 143 - 149 *

Also Published As

Publication number Publication date
AU5446694A (en) 1994-05-24
WO1994010315A2 (en) 1994-05-11

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