WO1994001563B1 - Recombinant dna encoding a desulfurization biocatalyst - Google Patents
Recombinant dna encoding a desulfurization biocatalystInfo
- Publication number
- WO1994001563B1 WO1994001563B1 PCT/US1993/006497 US9306497W WO9401563B1 WO 1994001563 B1 WO1994001563 B1 WO 1994001563B1 US 9306497 W US9306497 W US 9306497W WO 9401563 B1 WO9401563 B1 WO 9401563B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acid
- acid sequence
- dna
- orf
- set forth
- Prior art date
Links
- 230000003009 desulfurizing Effects 0.000 title claims abstract 6
- 108020004511 Recombinant DNA Proteins 0.000 title claims abstract 5
- 239000011942 biocatalyst Substances 0.000 title claims abstract 5
- 238000006477 desulfuration reaction Methods 0.000 title 1
- 229920003013 deoxyribonucleic acid Polymers 0.000 claims abstract 7
- 239000002803 fossil fuel Substances 0.000 claims abstract 5
- 241000187693 Rhodococcus rhodochrous Species 0.000 claims abstract 4
- 125000001741 organic sulfur group Chemical group 0.000 claims abstract 4
- 229920001850 Nucleic acid sequence Polymers 0.000 claims 9
- 150000007523 nucleic acids Chemical group 0.000 claims 5
- 108020004711 Nucleic Acid Probes Proteins 0.000 claims 3
- 125000003275 alpha amino acid group Chemical group 0.000 claims 3
- 239000002853 nucleic acid probe Substances 0.000 claims 3
- 101710022895 Asuc_1390 Proteins 0.000 claims 2
- 101710028935 BMEA_A0033 Proteins 0.000 claims 2
- 101710016828 MT2382 Proteins 0.000 claims 2
- 101710044466 NGR_a01600 Proteins 0.000 claims 2
- 101700050337 ORF 1 Proteins 0.000 claims 2
- 101700033410 PABL Proteins 0.000 claims 2
- 101700068398 PABT Proteins 0.000 claims 2
- 101700061424 POLB Proteins 0.000 claims 2
- 101710042866 R1A1-element\ORF1 Proteins 0.000 claims 2
- 101710041088 SPAC16C9.04c Proteins 0.000 claims 2
- 101710013770 SPBC2A9.03 Proteins 0.000 claims 2
- 101710011163 SYNPCC7002_B0001 Proteins 0.000 claims 2
- 101710005973 Smlt4048 Proteins 0.000 claims 2
- 101700037388 T1106 Proteins 0.000 claims 2
- 101710028952 TP_0022 Proteins 0.000 claims 2
- 101710015310 VI Proteins 0.000 claims 2
- 101710013750 YGL081W Proteins 0.000 claims 2
- 101700045543 YGY3 Proteins 0.000 claims 2
- 101700011797 YIN1 Proteins 0.000 claims 2
- 101700039061 YLF1 Proteins 0.000 claims 2
- 101700017361 YML1 Proteins 0.000 claims 2
- 101700005822 YNG1 Proteins 0.000 claims 2
- 101700043161 YOR3 Proteins 0.000 claims 2
- 101700053221 YPNR Proteins 0.000 claims 2
- 101700087083 YSP1 Proteins 0.000 claims 2
- 108060009635 YSP2 Proteins 0.000 claims 2
- 101700034306 YTR1 Proteins 0.000 claims 2
- 101700035048 YTR3 Proteins 0.000 claims 2
- 101700058339 YTX1 Proteins 0.000 claims 2
- 101700075752 YXYB Proteins 0.000 claims 2
- 101710004728 Zmob_1874 Proteins 0.000 claims 2
- 101700015203 bslA Proteins 0.000 claims 2
- 101700045377 mvp1 Proteins 0.000 claims 2
- 101700009423 ycf24 Proteins 0.000 claims 2
- 101700086855 yiaL Proteins 0.000 claims 2
- 101700026441 ymel-1 Proteins 0.000 claims 2
Abstract
This invention relates to a recombinant DNA molecule containing a gene or genes which encode a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules. For example, the present invention encompasses a recombinant DNA molecule containing a gene or genes of a strain of Rhodococcus rhodochrous.
Claims
AMENDED CLAIMS
[received by the International Bureau on 4 February 1994 (04.02.94); original claims 39-43 amended; remaining claims unchanged (2 pages)]
35. The method of desulfurizing a fossil fuel of Claim 33 wherein the DNA is derived from the IGTS8 strain of Rhodococcus rhodochrous bacteria.
36. A nucleic acid probe capable of hybridizing to all or a portion of a recombinant DNA molecule containing the gene of a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules.
37. A nucleic acid probe capable of hybridizing to all or a portion of a recombinant DNA molecule containing the gene of a strain of Rhodococcus rhodochrous bacteria wherein said gene encodes a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules.
38. A nucleic acid probe capable of hybridizing to a all or a portion of a DNA derived from a strain of
Rhodococcus rhodochrous bacteria wherein said DNA encodes a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules.
39. The nucleic acid sequence set forth in SEQ ID NO: l.
40. The nucleic acid sequence set forth in SEQ ID NO: 2.
41. The nucleic acid sequence set forth in SEQ ID NO: 3.
42. The nucleic acid sequence set forth in SEQ ID NO: 4.
43. The nucleic acid sequence set forth in SEQ ID NO: 5.
44. The DNA sequence for ORF-1.
90
45. The DNA sequence for ORF-2.
46. The DNA sequence for ORF-3.
47. The amino acid sequence for ORF-1.
48. The amino acid sequence for ORF-2.
49. The amino acid sequence for ORF-3.
50. The DNA sequences set forth starting on page 66 through page 80.
Priority Applications (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE69331323T DE69331323D1 (en) | 1992-07-10 | 1993-07-09 | RECOMBINANT DNA ENCODING A DESULFURING BIOCATALYST |
| KR1019950700119A KR950702632A (en) | 1992-07-10 | 1993-07-09 | RECOMBINANT DNA ENCODING A DESULFURIZATION BIOCATALYST |
| AU46718/93A AU684253B2 (en) | 1992-07-10 | 1993-07-09 | Recombinant dna encoding a desulfurization biocatalyst |
| AT93917083T ATE210726T1 (en) | 1992-07-10 | 1993-07-09 | RECOMBINANT DNA ENCODING A DESULFURIZATION BIOCATALYST |
| BR9306856A BR9306856A (en) | 1992-07-10 | 1993-07-09 | Recombinant dna molecule purified dna vector recombinant dna plasmid vector dna transformed microorganism transformed bacteria method for desulfurizing a fossil fuel nucleic acid probe and recombinant protein |
| JP6503543A JP2979178B2 (en) | 1992-07-10 | 1993-07-09 | Recombinant DNA encoding biocatalyst for desulfurization |
| EP93917083A EP0651808B1 (en) | 1992-07-10 | 1993-07-09 | Recombinant dna encoding a desulfurization biocatalyst |
| NO950081A NO950081L (en) | 1992-07-10 | 1995-01-09 | Recombinant DNA encoding a desulfurization biocatalyst |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US91184592A | 1992-07-10 | 1992-07-10 | |
| US07/911,845 | 1992-07-10 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1994001563A1 WO1994001563A1 (en) | 1994-01-20 |
| WO1994001563B1 true WO1994001563B1 (en) | 1994-03-03 |
Family
ID=25430955
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1993/006497 WO1994001563A1 (en) | 1992-07-10 | 1993-07-09 | Recombinant dna encoding a desulfurization biocatalyst |
Country Status (13)
| Country | Link |
|---|---|
| US (3) | US5356801A (en) |
| EP (1) | EP0651808B1 (en) |
| JP (1) | JP2979178B2 (en) |
| KR (1) | KR950702632A (en) |
| CN (1) | CN1085254A (en) |
| AT (1) | ATE210726T1 (en) |
| AU (1) | AU684253B2 (en) |
| BR (1) | BR9306856A (en) |
| CA (1) | CA2139876A1 (en) |
| DE (1) | DE69331323D1 (en) |
| MX (1) | MX9304135A (en) |
| RU (1) | RU95105971A (en) |
| WO (1) | WO1994001563A1 (en) |
Families Citing this family (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0563142B1 (en) * | 1990-12-21 | 1995-12-13 | Energy Biosystems Corporation | Use of a biocatalyst for the reduction of petroleum viscosity |
| US5510265A (en) * | 1991-03-15 | 1996-04-23 | Energy Biosystems Corporation | Multistage process for deep desulfurization of a fossil fuel |
| EP0584281B1 (en) * | 1991-05-01 | 1995-03-22 | Energy Biosystems Corporation | System and continuous process for biocatalytic desulfurization of sulfur-bearing heterocyclic molecules |
| US5458752A (en) * | 1993-09-03 | 1995-10-17 | Martin Marietta Energy Systems, Inc. | Apparatus and method for the desulfurization of petroleum by bacteria |
| US5468626A (en) * | 1993-12-17 | 1995-11-21 | Energy Biosystems Corporation | Method for separating a sulfur compound from carbonaceous materials |
| RU2053300C1 (en) * | 1993-12-17 | 1996-01-27 | Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов | Strain of bacterium rhodococcus rhodochrous - a producer of nitrile hydratase |
| US6995017B1 (en) | 1994-02-17 | 2006-02-07 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US6117679A (en) | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US6309883B1 (en) | 1994-02-17 | 2001-10-30 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US6335160B1 (en) * | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US5837458A (en) * | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US20060257890A1 (en) * | 1996-05-20 | 2006-11-16 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US6395547B1 (en) | 1994-02-17 | 2002-05-28 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US6165793A (en) * | 1996-03-25 | 2000-12-26 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| AU693633B2 (en) * | 1994-12-08 | 1998-07-02 | Energy Biosystems Corporation | Method of desulfurization of fossil fuel with flavoprotein |
| CN1198187A (en) * | 1995-09-21 | 1998-11-04 | 能量生物系统公司 | DszD utilization in desulfurization of DBT by rhodococcus sp. IGTS8 |
| FR2744455B1 (en) * | 1996-02-02 | 1998-04-24 | Sanofi Sa | NOVEL NUCLEIC ACID SEQUENCES OF THE FAMILY OF TUMOR SUPPRESSOR FACTORS, CORRESPONDING EXPRESSION PRODUCTS AND THEIR APPLICATIONS |
| US6506602B1 (en) | 1996-03-25 | 2003-01-14 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US5772901A (en) * | 1996-04-30 | 1998-06-30 | Energy Biosystems Corporation | Oil/water/biocatalyst three phase separation process |
| US5804433A (en) * | 1996-10-23 | 1998-09-08 | Energy Biosystems Corporation | Rhodococcus flavin reductase complementing DszA and DszC activity |
| US20070009930A1 (en) * | 1996-12-18 | 2007-01-11 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
| US6153410A (en) * | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
| US6133016A (en) * | 1997-04-07 | 2000-10-17 | Energy Biosystems Corporation | Sphingomonas biodesulfurization catalyst |
| US5952208A (en) * | 1997-04-07 | 1999-09-14 | Energy Biosystems Corporation | Dsz gene expression in pseudomonas hosts |
| US6071738A (en) * | 1997-09-19 | 2000-06-06 | Energy Biosystems Corporation | Conversion of organosulfur compounds to oxyorganosulfur compounds for desulfurization of fossil fuels |
| US7030227B1 (en) | 1997-10-15 | 2006-04-18 | President & Fellows Of Harvard College | Cell regulatory genes, encoded products, and uses related thereto |
| US7294459B1 (en) | 1997-10-15 | 2007-11-13 | President And Fellows Of Harvard College | Cell regulatory genes, encoded products, and uses related thereto |
| EP1036198B1 (en) | 1997-12-08 | 2012-09-26 | California Institute Of Technology | Method for creating polynucleotide and polypeptide sequences |
| US5968812A (en) * | 1998-02-02 | 1999-10-19 | Energy Biosystems Corporation | Removal of sulfinic acids |
| US6235519B1 (en) | 1998-02-26 | 2001-05-22 | Energy Biosystems Corporation | Gene involved in thiophene biotransformation from nocardia asteroides KGB1 |
| US5973195A (en) * | 1998-03-19 | 1999-10-26 | Energy Biosystems Corporation | Surfactants derived from 2-(2-hydroxyphenyl)benzenesulfinate and alkyl-substituted derivatives |
| JPH11341987A (en) * | 1998-04-02 | 1999-12-14 | Petroleum Energy Center Found | Genes coding for desulfurizing enzymes |
| US6365408B1 (en) | 1998-06-19 | 2002-04-02 | Maxygen, Inc. | Methods of evolving a polynucleotides by mutagenesis and recombination |
| WO2000042005A1 (en) | 1999-01-14 | 2000-07-20 | Energy Biosystems Corporation | Compositions comprising 2-(2-hydroxyphenyl) benzenesulfinate and alkyl-substituted derivatives thereof |
| ITMI20021217A1 (en) * | 2002-06-05 | 2003-12-05 | Enitecnologie Spa | PROMOTER AND EXPRESSIVE VECTOR IN RHODOCOCCUS AND ESCHERICHIA COLI |
| AUPS282802A0 (en) * | 2002-06-07 | 2002-06-27 | Commonwealth Scientific And Industrial Research Organisation | Novel enzymes |
| US8716464B2 (en) * | 2009-07-20 | 2014-05-06 | Thomas W. Geisbert | Compositions and methods for silencing Ebola virus gene expression |
| EP3339399A1 (en) * | 2016-12-22 | 2018-06-27 | Rainer Tesch | A method for treating petroleum or natural gas |
| CN110358743A (en) * | 2019-06-03 | 2019-10-22 | 华南理工大学 | A kind of monooxygenase DszC mutant and its preparation method and application |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4562156A (en) * | 1983-07-11 | 1985-12-31 | Atlantic Research Corporation | Mutant microorganism and its use in removing organic sulfur compounds |
| US4952500A (en) * | 1988-02-01 | 1990-08-28 | University Of Georgia Research Foundation, Inc. | Cloning systems for Rhodococcus and related bacteria |
| US5198341A (en) * | 1990-01-05 | 1993-03-30 | Institute Of Gas Technology | Useful for cleavage of organic C-S bonds Bacillus sphaericus microorganism |
| US5104801A (en) * | 1990-01-05 | 1992-04-14 | Institute Of Gas Technology | Mutant microorganisms useful for cleavage of organic c-s bonds |
| US5002888A (en) * | 1990-01-05 | 1991-03-26 | Institute Of Gas Technology | Mutant microorganisms useful for cleavage of organic C-S bonds |
| US5132219A (en) * | 1990-02-28 | 1992-07-21 | Institute Of Gas Technology | Enzymes from Rhodococcus rhodochrous strain ATCC No. 53968, Bacillus sphaericus strain ATCC No. 53969 and mixtures thereof for cleavage of organic C--S bonds of carbonaceous material |
| US5232854A (en) * | 1991-03-15 | 1993-08-03 | Energy Biosystems Corporation | Multistage system for deep desulfurization of fossil fuels |
| EP0584281B1 (en) * | 1991-05-01 | 1995-03-22 | Energy Biosystems Corporation | System and continuous process for biocatalytic desulfurization of sulfur-bearing heterocyclic molecules |
-
1993
- 1993-07-09 RU RU95105971/13A patent/RU95105971A/en unknown
- 1993-07-09 WO PCT/US1993/006497 patent/WO1994001563A1/en active IP Right Grant
- 1993-07-09 AU AU46718/93A patent/AU684253B2/en not_active Ceased
- 1993-07-09 BR BR9306856A patent/BR9306856A/en not_active Application Discontinuation
- 1993-07-09 US US08/089,755 patent/US5356801A/en not_active Expired - Lifetime
- 1993-07-09 EP EP93917083A patent/EP0651808B1/en not_active Expired - Lifetime
- 1993-07-09 KR KR1019950700119A patent/KR950702632A/en active IP Right Grant
- 1993-07-09 DE DE69331323T patent/DE69331323D1/en not_active Expired - Lifetime
- 1993-07-09 JP JP6503543A patent/JP2979178B2/en not_active Expired - Lifetime
- 1993-07-09 AT AT93917083T patent/ATE210726T1/en not_active IP Right Cessation
- 1993-07-09 CA CA002139876A patent/CA2139876A1/en not_active Abandoned
- 1993-07-09 MX MX9304135A patent/MX9304135A/en not_active IP Right Cessation
- 1993-07-10 CN CN93116500A patent/CN1085254A/en active Pending
-
1995
- 1995-04-14 US US08/421,791 patent/US5879914A/en not_active Expired - Lifetime
- 1995-04-14 US US08/421,754 patent/US5578478A/en not_active Expired - Fee Related
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