WO1994000584A3 - Artemisia annua transformed with agrobacterium rhizogenes - Google Patents

Artemisia annua transformed with agrobacterium rhizogenes Download PDF

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Publication number
WO1994000584A3
WO1994000584A3 PCT/US1993/005950 US9305950W WO9400584A3 WO 1994000584 A3 WO1994000584 A3 WO 1994000584A3 US 9305950 W US9305950 W US 9305950W WO 9400584 A3 WO9400584 A3 WO 9400584A3
Authority
WO
WIPO (PCT)
Prior art keywords
annua
transformed
transformation
artemisia annua
agrobacterium rhizogenes
Prior art date
Application number
PCT/US1993/005950
Other languages
French (fr)
Other versions
WO1994000584A2 (en
Inventor
Pamela J Weathers
Ronald D Cheetham
Alexander Diiorio
Original Assignee
Worcester Polytech Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Worcester Polytech Inst filed Critical Worcester Polytech Inst
Publication of WO1994000584A2 publication Critical patent/WO1994000584A2/en
Publication of WO1994000584A3 publication Critical patent/WO1994000584A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
    • C07D493/12—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains three hetero rings
    • C07D493/20—Spiro-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/181—Heterocyclic compounds containing oxygen atoms as the only ring heteroatoms in the condensed system, e.g. Salinomycin, Septamycin

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A method of transforming Artemisia annua with Agrobacterium rhizogenes. More particularly, a method for producing an Agrobacterium rhyzogenes hairy root cell culture of Artemisia annua. The method of transformation of A. annua includes the transformation of whole plants with A. rhyzogenes, and also includes transformation of A. annua root cultures with A. rhizogenes. The present invention also relates to transformed A. annua. The present invention also relates to a method for extracting enhanced amounts of a plant secondary metabolite from plant tissue with limited loss of tissue viability by reversibly permeabilizing the tissue membrane is disclosed.
PCT/US1993/005950 1992-06-23 1993-06-22 Artemisia annua transformed with agrobacterium rhizogenes WO1994000584A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US90291692A 1992-06-23 1992-06-23
US07/902,916 1992-06-23

Publications (2)

Publication Number Publication Date
WO1994000584A2 WO1994000584A2 (en) 1994-01-06
WO1994000584A3 true WO1994000584A3 (en) 1994-03-31

Family

ID=25416616

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1993/005950 WO1994000584A2 (en) 1992-06-23 1993-06-22 Artemisia annua transformed with agrobacterium rhizogenes

Country Status (1)

Country Link
WO (1) WO1994000584A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9606579D0 (en) * 1996-03-28 1996-06-05 Phytotech Ltd Pharmaceutical composition and methods for the manufacture thereof
EP0982404A1 (en) * 1998-08-27 2000-03-01 Wallaart, Thorvald Eelco DNA encoding amorpha-4,11-diene synthase
CN102127566B (en) * 2010-12-17 2012-04-18 湖南农业大学 Genetic transformation method for artemisia annua
CN104651373B (en) * 2015-02-03 2018-02-09 中国人民解放军第二军医大学 The albumen and application of sweet wormwood AaGTD1 genes and its coding
US11299700B1 (en) 2021-02-19 2022-04-12 Acequia Biotechnology, Llc Bioreactor containers and methods of growing hairy roots using the same
CN113832178A (en) * 2021-09-08 2021-12-24 浙江省农业科学院 Method for establishing agrobacterium rhizogenes-mediated vegetable pea genetic transformation system

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4952603A (en) * 1988-06-20 1990-08-28 Elferaly Farouk S Method for the isolation of artemisinin from Artemisia annua

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4952603A (en) * 1988-06-20 1990-08-28 Elferaly Farouk S Method for the isolation of artemisinin from Artemisia annua

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DIIORIO, A.A., ET AL.: "Modelling of hairy root growth", ABSTRACTS OF PAPERS OF AMERICAN CHEMICAL SOCIETY, vol. 205, no. 1-2, 1993, pages BIOT152 *
FLORES, H.E., ET AL.: "Production of polyacetylenes and thiopenes in heterotrophic and photosynthetic root cultures of Asteraceae", CHEMISTRY AND BIOLOGY OF NATURALLY OCCURRING ACETYLENES AND RELATED COMPOUNDS, 1988, EDITED BY J. LAM, ET AL., ELSEVIER AMSTREDAM., pages 233 - 254 *
HAMILL, J.D., ET AL.: "New routes to plant secondary products", BIOTECHNOLOGY, vol. 5, 1987, NEW YORK US, pages 800 - 804, XP002008146, DOI: doi:10.1038/nbt0887-800 *
NAIR, M.S.R., ET AL.: "Production of artemisinin in tissue cultures of Aretemisia annua", JOURNAL OF NATURAL PRODUCTS, vol. 49, no. 3, 1986, pages 504 - 507, XP001344656 *
PARR, A.J.: "The production of secondary metabolites by plant cell cultures", JOURNAL OF BIOTECHNOLOGY, vol. 10, 1989, AMSTERDAM NL, pages 1 - 26, XP023698791, DOI: doi:10.1016/0168-1656(89)90089-8 *
SIGNS, M.W., ET AL.: "The biosynthetic potential of plant roots", BIOESSAYS, vol. 12, no. 1, January 1990 (1990-01-01), pages 7 - 13 *

Also Published As

Publication number Publication date
WO1994000584A2 (en) 1994-01-06

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