WO1993022463A3 - Methods for high resolution gene mapping - Google Patents

Methods for high resolution gene mapping Download PDF

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Publication number
WO1993022463A3
WO1993022463A3 PCT/US1993/004330 US9304330W WO9322463A3 WO 1993022463 A3 WO1993022463 A3 WO 1993022463A3 US 9304330 W US9304330 W US 9304330W WO 9322463 A3 WO9322463 A3 WO 9322463A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
chromosome
high resolution
discrete
dna sequences
Prior art date
Application number
PCT/US1993/004330
Other languages
French (fr)
Other versions
WO1993022463A2 (en
Inventor
Timothy W Houseal
Original Assignee
Ig Lab Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ig Lab Inc filed Critical Ig Lab Inc
Priority to JP5519641A priority Critical patent/JPH07506259A/en
Priority to EP93911141A priority patent/EP0640147A1/en
Publication of WO1993022463A2 publication Critical patent/WO1993022463A2/en
Publication of WO1993022463A3 publication Critical patent/WO1993022463A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Two convenient methods for ordering discrete DNA sequences at a high resolution are described. In one embodiment, the invention features a) contacting a eukaryotic cell containing a chromosome of interest with an agent that effects decondensation; b) preparing the cell containing decondensed chromatin for hybridization; and c) hybridizing the cell produced in step b) with at least two DNA probes, each probe being complementary to at least one discrete DNA sequence present on the chromosome of interest; and d) detecting the presence and relative order of the probes as an indication of the relative and absolute locations of the DNA sequences on the chromosome. In another embodiment, the invention features ordering at least two discrete DNA sequences on a fragment of DNA that has been extended, for example by physical means, chemical means or a combination of both. A preferred physical means for extending DNA is by gentle smearing.
PCT/US1993/004330 1992-05-07 1993-05-07 Methods for high resolution gene mapping WO1993022463A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP5519641A JPH07506259A (en) 1992-05-07 1993-05-07 High-resolution gene mapping method
EP93911141A EP0640147A1 (en) 1992-05-07 1993-05-07 Methods for high resolution gene mapping

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US88014592A 1992-05-07 1992-05-07
US07/880,145 1992-05-07

Publications (2)

Publication Number Publication Date
WO1993022463A2 WO1993022463A2 (en) 1993-11-11
WO1993022463A3 true WO1993022463A3 (en) 1993-12-09

Family

ID=25375603

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1993/004330 WO1993022463A2 (en) 1992-05-07 1993-05-07 Methods for high resolution gene mapping

Country Status (5)

Country Link
EP (1) EP0640147A1 (en)
JP (1) JPH07506259A (en)
AU (1) AU4238593A (en)
CA (1) CA2135072A1 (en)
WO (1) WO1993022463A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2716263B1 (en) * 1994-02-11 1997-01-17 Pasteur Institut Method for aligning macromolecules by passing a meniscus and applications in a method for highlighting, separating and / or assaying a macromolecule in a sample.
US6248537B1 (en) 1999-05-28 2001-06-19 Institut Pasteur Use of the combing process for the identification of DNA origins of replication
US6927065B2 (en) 1999-08-13 2005-08-09 U.S. Genomics, Inc. Methods and apparatus for characterization of single polymers
US6762059B2 (en) 1999-08-13 2004-07-13 U.S. Genomics, Inc. Methods and apparatuses for characterization of single polymers
US6696022B1 (en) 1999-08-13 2004-02-24 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
GB0016920D0 (en) * 2000-07-10 2000-08-30 Univ Cambridge Tech Decondensation of DNA
EP1546380A4 (en) 2002-05-28 2007-02-14 Us Genomics Inc Methods and apparati using single polymer analysis
US9028776B2 (en) 2012-04-18 2015-05-12 Toxic Report Llc Device for stretching a polymer in a fluid sample

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CYTOGENETICS AND CELL GENETICS vol. 53, no. 4, 1990, BASEL, SWITZERLAND pages 219 - 220 MOENS, P.E. ET AL 'in situ DNA sequence mapping with surface-spread mouse pachytene chromosomes' *
GENOMICS vol. 10, May 1991, SAN DIEGO USA pages 75 - 82 BRANDRIFF, B. ET AL 'a new system for high-resolution DNA sequence mapping in interphase pronuclei' *
SCIENCE vol. 247, 5 January 1990, LANCASTER, PA US pages 64 - 68 LICHTER, P. ET AL 'high-resolution mapping of human chromosome 11' *
SCIENCE vol. 249, 24 August 1990, LANCASTER, PA. US pages 928 - 932 LAWRENCE, J.B. ET AL 'interphase and metaphase resolution of different distances within the human dystrophin gene' *
THE AMERICAN JOURNAL OF HUMAN GENETICS vol. 51, no. 4, October 1992, CHICAGO, USA page A9 HOUSEAL,T.W. ET AL 'sodium butyrate and histone depletion improve resolution and sensitivity of mapping by flourescence in situ hybridization' abstract 27 *

Also Published As

Publication number Publication date
WO1993022463A2 (en) 1993-11-11
EP0640147A1 (en) 1995-03-01
JPH07506259A (en) 1995-07-13
CA2135072A1 (en) 1993-11-11
AU4238593A (en) 1993-11-29

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