WO1993012805A1 - Procede de regulation des lignages cellulaires humains hematopoeïtiques - Google Patents

Procede de regulation des lignages cellulaires humains hematopoeïtiques Download PDF

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Publication number
WO1993012805A1
WO1993012805A1 PCT/US1992/011228 US9211228W WO9312805A1 WO 1993012805 A1 WO1993012805 A1 WO 1993012805A1 US 9211228 W US9211228 W US 9211228W WO 9312805 A1 WO9312805 A1 WO 9312805A1
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Prior art keywords
cells
human
medium
cell
culture
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PCT/US1992/011228
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English (en)
Inventor
Bernhard O. Palsson
R. Douglas Armstrong
Michael F. Clarke
Stephen G. Emerson
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Regents Of The University Of Michigan
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Publication of WO1993012805A1 publication Critical patent/WO1993012805A1/fr

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0642—Granulocytes, e.g. basopils, eosinophils, neutrophils, mast cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/5412—IL-6
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0641—Erythrocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/14—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1394—Bone marrow stromal cells; whole marrow

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

On décrit des procédés, y compris des conditions de milieux de culture, qui produisent la division des cellules-souches humaines in vitro et/ou l'optimisation des cultures des cellules-souches hématopoéïtiques humaines, et/ou l'accroissement du métabolisme ou de la sécrétion GM-CSF ou IL-6 ou des cellules stromales humaines, et/ou un procédé pour déterminer l'effet d'une substance ou l'état d'une population cellulaire hématopoéïtique humaine, et/ou pour provoquer la déplétion des cellules malignes ou le contenu des cellules T ou des cellules B d'une population cellulaire hématopoéïtique humaine. Les procédés s'appuient sur la culture des cellules-souches humaines et/ou des cellules-souches hématopoéïtiques humaines, et/ou des cellules stromales humaines dans un milieu de culture liquide qui est remplacé, de préférence irrigué, soit continuellement, soit périodiquement, à un taux de 1 ml de milieu par ml de culture par période d'environ 24 à environ 48 heures, et sur l'élimination de produits métaboliques et la recharge de produits nutritifs appauvris tout en maintenant la culture dans des conditions physiologiquement acceptables. Eventuellement, on ajoute des facteurs de croissance au milieu de culture. Les conditions de culture décrites permettent d'obtenir des procédés améliorés de transplantation de la moëlle osseuse.
PCT/US1992/011228 1992-01-02 1992-12-31 Procede de regulation des lignages cellulaires humains hematopoeïtiques WO1993012805A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US81551392A 1992-01-02 1992-01-02
US815,513 1992-01-02

Publications (1)

Publication Number Publication Date
WO1993012805A1 true WO1993012805A1 (fr) 1993-07-08

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PCT/US1992/011228 WO1993012805A1 (fr) 1992-01-02 1992-12-31 Procede de regulation des lignages cellulaires humains hematopoeïtiques

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WO (1) WO1993012805A1 (fr)

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5704888A (en) * 1995-04-14 1998-01-06 Cobe Laboratories, Inc. Intermittent collection of mononuclear cells in a centrifuge apparatus
US5704889A (en) * 1995-04-14 1998-01-06 Cobe Laboratories, Inc. Spillover collection of sparse components such as mononuclear cells in a centrifuge apparatus
US5763267A (en) * 1996-04-16 1998-06-09 Advanced Tissue Sciences Apparatus for the large scale growth and packaging of cell suspensions and three-dimensional tissue cultures
US5792603A (en) * 1995-04-27 1998-08-11 Advanced Tissue Sciences, Inc. Apparatus and method for sterilizing, seeding, culturing, storing, shipping and testing tissue, synthetic or native, vascular grafts
US5843766A (en) * 1995-06-07 1998-12-01 Advanced Tissue Sciences, Inc. Apparatus for the growth and packaging of three dimensional tissue cultures
US5846828A (en) * 1995-06-07 1998-12-08 Advanced Tissue Sciences Apparatus and method for sterilizing, seeding, culturing, storing, shipping, and testing tissue, synthetic, or mechanical heart valves orvalve segments
WO1999041382A2 (fr) * 1998-02-17 1999-08-19 Hyseq, Inc. Nouvelle interleukine 3 et ses utilisations
US6004812A (en) * 1994-12-01 1999-12-21 Beth Israel Medical Center, Inc. In-vitro T-lymphopoiesis system
US6060306A (en) * 1995-06-07 2000-05-09 Advanced Tissue Sciences, Inc. Apparatus and method for sterilizing, seeding, culturing, storing, shipping and testing replacement cartilage tissue constructs
US6121042A (en) * 1995-04-27 2000-09-19 Advanced Tissue Sciences, Inc. Apparatus and method for simulating in vivo conditions while seeding and culturing three-dimensional tissue constructs
WO2002072116A1 (fr) * 2001-03-08 2002-09-19 Max-Delbrück-Centrum für Molekulare Medizin Cellules sanguines transformees a l'aide de myb et leur utilisation pour le criblage de substances actives
WO2004043990A2 (fr) * 2002-11-07 2004-05-27 University Of Chicago Materiels a base de cellules souches humaines et procedes correspondant
US8835163B2 (en) 2006-10-18 2014-09-16 The Board Of Trustees Of The University Of Illinois Embryonic-like stem cells derived from adult human peripheral blood and methods of use
US9388382B2 (en) 2005-10-05 2016-07-12 The Board Of Trustees Of The University Of Illinois Isolation of CD14 negative, CD45 positive and CD117 positive embryonic-like stem cells free of monocytes from human umbilical cord blood mononuclear cells

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5032508A (en) * 1988-09-08 1991-07-16 Marrow-Tech, Inc. Three-dimensional cell and tissue culture system

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5032508A (en) * 1988-09-08 1991-07-16 Marrow-Tech, Inc. Three-dimensional cell and tissue culture system

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CANADIAN JOURNAL OF CHEMICAL ENGINEERING, Volume 64, issued August 1986, ADAMSON et al., "Industrial Mammalian Cell Culture", pages 531-539. *
CELL, Volume 47, issued 10 October 1986, YANG et al., "Human IL-3 (Multi-CSF): Identification by Expression Cloning of a Novel Hematopoietic Growth Factor Related to Murine IL-3", pages 3-10. *
TRENDS IN BIOTECHNOLOGY, Volume 1, No. 4, issued 1983, GLACKEN et al., "Mammalian Cell Culture: Engineering Principles and Scale-Up", pages 102-108. *

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6004812A (en) * 1994-12-01 1999-12-21 Beth Israel Medical Center, Inc. In-vitro T-lymphopoiesis system
US5879280A (en) * 1995-04-14 1999-03-09 Cobe Laboratories, Inc. Intermittent collection of mononuclear cells in a centrifuge apparatus
US5704889A (en) * 1995-04-14 1998-01-06 Cobe Laboratories, Inc. Spillover collection of sparse components such as mononuclear cells in a centrifuge apparatus
US5704888A (en) * 1995-04-14 1998-01-06 Cobe Laboratories, Inc. Intermittent collection of mononuclear cells in a centrifuge apparatus
US5876321A (en) * 1995-04-14 1999-03-02 Cobe Laboratories, Inc. Control system for the spillover collection of sparse components such as mononuclear cells in a centrifuge apparatus
US6121042A (en) * 1995-04-27 2000-09-19 Advanced Tissue Sciences, Inc. Apparatus and method for simulating in vivo conditions while seeding and culturing three-dimensional tissue constructs
US5792603A (en) * 1995-04-27 1998-08-11 Advanced Tissue Sciences, Inc. Apparatus and method for sterilizing, seeding, culturing, storing, shipping and testing tissue, synthetic or native, vascular grafts
US5843766A (en) * 1995-06-07 1998-12-01 Advanced Tissue Sciences, Inc. Apparatus for the growth and packaging of three dimensional tissue cultures
US5846828A (en) * 1995-06-07 1998-12-08 Advanced Tissue Sciences Apparatus and method for sterilizing, seeding, culturing, storing, shipping, and testing tissue, synthetic, or mechanical heart valves orvalve segments
US6060306A (en) * 1995-06-07 2000-05-09 Advanced Tissue Sciences, Inc. Apparatus and method for sterilizing, seeding, culturing, storing, shipping and testing replacement cartilage tissue constructs
US5763267A (en) * 1996-04-16 1998-06-09 Advanced Tissue Sciences Apparatus for the large scale growth and packaging of cell suspensions and three-dimensional tissue cultures
WO1999041382A2 (fr) * 1998-02-17 1999-08-19 Hyseq, Inc. Nouvelle interleukine 3 et ses utilisations
WO1999041382A3 (fr) * 1998-02-17 1999-12-16 Hyseq Inc Nouvelle interleukine 3 et ses utilisations
WO2002072116A1 (fr) * 2001-03-08 2002-09-19 Max-Delbrück-Centrum für Molekulare Medizin Cellules sanguines transformees a l'aide de myb et leur utilisation pour le criblage de substances actives
WO2004043990A2 (fr) * 2002-11-07 2004-05-27 University Of Chicago Materiels a base de cellules souches humaines et procedes correspondant
WO2004043990A3 (fr) * 2002-11-07 2005-02-03 Univ Chicago Materiels a base de cellules souches humaines et procedes correspondant
US9388382B2 (en) 2005-10-05 2016-07-12 The Board Of Trustees Of The University Of Illinois Isolation of CD14 negative, CD45 positive and CD117 positive embryonic-like stem cells free of monocytes from human umbilical cord blood mononuclear cells
US8835163B2 (en) 2006-10-18 2014-09-16 The Board Of Trustees Of The University Of Illinois Embryonic-like stem cells derived from adult human peripheral blood and methods of use

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Publication number Publication date
AU3422893A (en) 1993-07-28

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