WO1992008793A1 - Chinese hamster ovary cell retrovirus-like c particles - Google Patents
Chinese hamster ovary cell retrovirus-like c particles Download PDFInfo
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- WO1992008793A1 WO1992008793A1 PCT/US1991/008254 US9108254W WO9208793A1 WO 1992008793 A1 WO1992008793 A1 WO 1992008793A1 US 9108254 W US9108254 W US 9108254W WO 9208793 A1 WO9208793 A1 WO 9208793A1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
- C12N2740/13021—Viruses as such, e.g. new isolates, mutants or their genomic sequences
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
- C12N2740/13022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Definitions
- FIG. 1 Electron micrographs of double-gradient purified particles from CHO 3-3000-44 cells. RT containing fractions from the second gradient of a double-gradient purification were pooled, concentrated, and dialyzed. After spotting onto EM grids, particles were visualized by negative staining using uranyl acetate. Shown are three examples of the particles observed. The size and morphology are consistent with previous descriptions of retrovirus particles visualized by this technique. Figure 3. Retrovirus-related proteins and nucleic acids in gradient purified particles.
- Novel nucleic acid compositions are provided herewith.
- This invention encompasses the following DNA sequence (Seq. ID No. 1 ) which encodes an RNA transcript of a C-type retrovirus-like particle endogenous in CHO cells.
- the activity of the cell lysate or purified particle variant is then screened in a suitable screening assay for the desired characteristic.
- a change in the character of the particle such as retroviral activity, is measured by standard assays.
- other assays will become useful in such screening. Modifications of such protein properties as redox or thermal stability, hydrophobicity, susceptibility to proteolytic degradation, or the tendency to aggregate with carriers or into multimers are assayed by methods well known to the artisan.
- eukaryotic microbes such as yeast or filamentous fungi are satisfactory.
- Saccharomvces cerevisiae is the most commonly used eukaryotic microorganism, although a number of other strains are commonly available.
- the plasmid YRp7 is a satisfactory expression vector in yeast (Stinchcomb, et al. ,, Nature 282: 39 [1 979]; Kingsman et a/., Gene 7: 141 [1 979]; Tschemper et a/., Gene 10: 1 57 [1 980]).
- Suitable promoting sequences for use with yeast hosts include the promoters for 3- phosphoglycerate kinase (Hitzeman et a/., “J. Biol. Chem.” 255: 2073 [1980]) or other glycolytic enzymes (Hess et al., "J. Adv.
Abstract
Description
Claims
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JP4502635A JPH06501850A (en) | 1990-11-09 | 1991-11-07 | Retrovirus-like particles in Chinese hamster ovary cells |
Applications Claiming Priority (2)
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US61152990A | 1990-11-09 | 1990-11-09 | |
US611,529 | 1990-11-09 |
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WO1992008793A1 true WO1992008793A1 (en) | 1992-05-29 |
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ID=24449391
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PCT/US1991/008254 WO1992008793A1 (en) | 1990-11-09 | 1991-11-07 | Chinese hamster ovary cell retrovirus-like c particles |
Country Status (4)
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EP (1) | EP0556339A1 (en) |
JP (1) | JPH06501850A (en) |
CA (1) | CA2095635A1 (en) |
WO (1) | WO1992008793A1 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7632509B2 (en) * | 2005-07-19 | 2009-12-15 | Biosante Pharmaceuticals, Inc. | Methods to express recombinant proteins from lentiviral vectors |
US7662623B2 (en) | 2003-06-03 | 2010-02-16 | Biosante Pharmaceuticals, Inc. | Compositions and methods for enhanced expression of recombinant polypeptides from a single vector using a peptide cleavage site |
US7714119B2 (en) | 2004-07-13 | 2010-05-11 | Biosante Pharmaceuticals, Inc. | AAV vector compositions and methods for enhanced expression of immunoglobulins using the same |
Families Citing this family (1)
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EP2041296A4 (en) * | 2006-05-04 | 2010-11-03 | Abmaxis Inc | Method for producing stable mammalian cell lines producing high levels of recombinant proteins |
-
1991
- 1991-11-07 EP EP19920902210 patent/EP0556339A1/en not_active Withdrawn
- 1991-11-07 JP JP4502635A patent/JPH06501850A/en active Pending
- 1991-11-07 WO PCT/US1991/008254 patent/WO1992008793A1/en not_active Application Discontinuation
- 1991-11-07 CA CA 2095635 patent/CA2095635A1/en not_active Abandoned
Non-Patent Citations (4)
Title |
---|
CHEMICAL ABSTRACTS, vol. 112, 1990, Columbus, Ohio, US; abstract no. 211683U, ANDERSON, K. P. ET AL.: 'Presence and transcription of intracisternal A-particle-related sequences in CHO cells' page 142 ;column 1 ; * |
JOURNAL OF GENERAL VIROLOGY vol. 44, 1979, pages 45 - 55; HEINE, U.I. ET AL.: 'Enhanced proliferation of endogenous virus in Chinese hamster cells associated with microtubules and the mitotic apparatus of the host cells' * |
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA. vol. 75, no. 2, February 1978, WASHINGTON US pages 1004 - 1008; TODARO, G.J. ET AL.: 'Endogenous new world primate type C viruses isolated from owl monkey (Aous trivirgatus) kidney cell line' * |
VIROLOGY vol. 181, no. 1, March 1991, pages 305 - 311; ANDERSON, K.P. ET AL.: 'Endogenous origin of defctive retroviruslike particles from a recombinant Chinese Hamster Ovary cell line' * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7662623B2 (en) | 2003-06-03 | 2010-02-16 | Biosante Pharmaceuticals, Inc. | Compositions and methods for enhanced expression of recombinant polypeptides from a single vector using a peptide cleavage site |
US7709224B2 (en) | 2003-06-03 | 2010-05-04 | Biosante Pharmaceuticals, Inc. | Compositions and methods for enhanced expression of recombinant polypeptides from a single vector using a peptide cleavage site |
US7714119B2 (en) | 2004-07-13 | 2010-05-11 | Biosante Pharmaceuticals, Inc. | AAV vector compositions and methods for enhanced expression of immunoglobulins using the same |
US7632509B2 (en) * | 2005-07-19 | 2009-12-15 | Biosante Pharmaceuticals, Inc. | Methods to express recombinant proteins from lentiviral vectors |
Also Published As
Publication number | Publication date |
---|---|
CA2095635A1 (en) | 1992-05-10 |
EP0556339A1 (en) | 1993-08-25 |
JPH06501850A (en) | 1994-03-03 |
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