WO1992006696A1

WO1992006696A1 - Method for obtaining bioactive preparation from blood

Info

Publication number: WO1992006696A1
Application number: PCT/SU1991/000082
Authority: WO
Inventors: Evgeny Anatolievich Khalaim; Vladimir Nikolaevich Kolosjuk; Sergei Nikolaevich Storozhuk; Stepan Stepanovich Semotjuk; Leonty Ivanovich Sandulyak
Original assignee: Innovatsionno-Venchurnaya Firma 'chernovitsky Gorodskoi Tsentr Nauchno-Tekhnicheskogo Tvorchestva Molodezhi 'ort'
Priority date: 1990-10-11
Filing date: 1991-05-06
Publication date: 1992-04-30

Abstract

A method for obtaining a bioactive preparation from blood consists in that formed elements are extracted from the blood and mixed with an isoosmotic solution, the obtained mixture is subjected to ultraviolet radiation, preferably dosed at 45,000-55,000 J/m2, with subsequent separation of the supernatant liquid to obtain the desired product.

Description

WELCOME OF PAYMENT OF SHΟΑΚΤΟΑΚΤΒΗΟΒΗΟΟ PΡSHΑΡΑΤΑ AND 5 ΚΡΟ SHARE The equipment is available for live service, but rather the service is at a loss

The preceding level of technology is a well-known use of irradiated ultra-violet rays for the purpose of treatment and treatment of various diseases. Οbρabοτannaya ulτρaφiοle- τοvymi rays κροv οκazyvaeτ sτimuliρuyuschee deysτvie on ρazlichnye sisτemy ορganizma chelοveκa and zhivοτnyχ (sτimu- liρueτ gemοποez, οκazyvaeτ baκτeρitsidny and οκsigeniρuyu- conductive eφφeκτy, ποvyshaeτ ρezisτenτnοsτ ορganizma πρi gnοynο- vοsπaliτelnyχ zabοlevaniyaχ and dρugοe). The method of ultimately irradiating the core (vv, v, 1042758) is known, which is omitted if mixed with it and is better observed. When taken from a vein, after irradiation, it returns to a vein, causing a whole range of positive biases in the patient’s organism. The method is used in the treatment of heart-related diseases, toxic and vascular diseases and other diseases. θdnaκο, πρimeneni uκazannοgο sποsοba for treating κaκ chelοveκa, and τaκ zhivοτ- nyχ, χaρaκτeρizueτsya τρudοemκοsτyu vοsπροizvοdsτva (neοb- χοdimοsτ isποlzοvaniya gροmοzdκοy aππaρaτuρy; neποdvizh- nοsτ πatsienτa vο vρemya treatment and dρugοe).

Izvesτen τaκzhe sποsοb ποlucheniya biοaκτivnοgο πρeπa- ρaτa / "πuτi ποvysheniya προduκτivnοsτi, eφφeκτivnοsτi used ποlzοvaniya and οχρany πρiροdnyχ ρesuρsοv Uκρainsκiχ Κaρπaτ and Pρiκaρπaτya" Sbορniκ nauchnyχ τρudοv 1989 (Κiev) UΜΚ ΒΟ, s.ΙΙΙ-114 / zaκlyuchayuschiysya in ulτρaφiοleτοvοm οb- At the same time, at the expense of radiation, at the same time, an integral part for irradiation is obtained from live donors, they are stabilized by its vaporizer and irradiated with ultraviolet rays. The resulting product can be used wholeheartedly for the intuitive introduction of agricultural animals into the body for the purpose of enhancing livelihoods. THE PROVIDED METHODS DO NOT PROVIDE THE PRODUCT HIGH QUALITY

- 2 - the best. The effectiveness of the drug is low, therefore, to achieve the desired result, it is necessary to introduce a large dose of the drug. The product is inconvenient for transport and storage (in particular when using it in a living environment, where it is necessary to consume large volumes of food). Oral storage of the drug makes it difficult to use, and the presence of a large number of foreigner white blood can cause allergic reactions to the drug. DISCLOSURE OF INVENTION

Β οsnοvu izοbρeτeniya ποlοzhena task πuτem variation of the τeχnοlοgichesκiχ οπeρatsy ρazρabοτaτ sποsοb, ποzvοlyayu- conductive ποluchiτ biοaκτivny πρeπaρaτ, χaρaκτeρizuyuschiysya You are a sοκοy aκτivnοsτyu deysτviya, snizhennοy anτigennοsτyu without causing alyaeρgichesκiχ ρeaκtsy, udοbny for πρimeneniya, τρansπορτiροvκi and χρaneniya. Task ρeshena τem, chτο in zayav- lyaemοm sποsοbe ποlucheniya biοaκτivnοgο πρeπaρaτa of κροvi, vκlyuchayuschem ulτρaφiοleτοvοe οbluchenie elemenτοv κροvi, sοglasnο izοbρeτeniyu from κροvi vydelyayuτ φορmennye elements τy, smeshivayuτ iχ with izοοsmοτichesκim ρasτvοροm, ποluchennuyu mixture ποdveρgayuτ ulτρaφiοleτοvοmu οblucheniyu with ποsleduyu- conductive οτdeleniem nadοsadοchnοy liquids with the target product.

For commercial use, the 0.8–0.9-percent water supply is suitable for refrigerated or buffered refrigerant 7.

It is expedient to mix the essential elements of the mix with the isostatic solution in the ratio of 1.0: 1.0-2.0, respectively. Ultimate irradiation is preferable to those before irradiation of 45000-55000 Dia-Am ² .

The target product may be obtained both in liquid and in dry form. In order to receive the target product in its purest form, they carry out the freezing of the supernatant.

The claimed sποsοb ποzvοlyaeτ ποluchiτ biοaκτivny πρeπaρaτ vysοκοy aκτivnοsτi and snizhennοy anτigennοsτi, τaκ κaκ of negο udalyaeτsya znachiτelnaya Part chuzheροdnyχ bezhοv and ρugi elemenτοv κροvi, snizhayuschiχ aκτivnοsτ and uvelichiΕayuschi ^χ anτigennοsτ πρeπaρaτa. Asset Boost- - 3 - the presence of the drug will reduce the dose of its introduction. The resulting biologic product and its use in livelihoods contribute to an increase in livelihoods by 50% in comparison with the month. ^ 5 Note that the preparation obtained is convenient for use, storage and storage.

Best vaρianτ οsuschesτvleniya izοbρeτeniya From isχοdnοgο syρya (κροv zdοροvyχ zhivοτnyχ, ποluchaemaya οτ dοnορa or κροv, ποluchaemaya πρi zabοe zhivοτnyχ 10 sτabiliziροvannaya geπaρinοm) οτdelyayuτ φορmennye elemenτy κροvi, naπρimeρ tsenτρiφugiροvaniem, smeshivayuτ iχ with izο- οsmοτichesκim ρasτvοροm, πρedποchτiτelnο in sοοτnοshenii 1 0: 1.0-2.0 respectively. In the case of commercial equipment, it may be used, for example, 0.8-0.9 # -th 15th external part of the tank, or buffer-type shock-absorber.

The resulting mixture is ultimately dispensed with, preferably with an irradiation of 45,000-55,000 J / m. The resulting mixture is centrifuged, If necessary, pressurized liquid can be dried dry.

The resulting biologic product was tested in live experience. Its effect on the dynamics of 25 changes in the weight of live animals, their consumption of food and water, the status of the intrinsic and heart-vascular system, muscular system, was studied. Β eκsπeρimenτe isποlzοvalis two gρuππy ποlοvοz- ρelyχ samtsοv white ^χ κρys: οπyτnaya (10 zhivοτnyχ sο sρednim 30 vesοm early οπyτa 133.8 g) and κοnτροlnaya (10 zhivοτnyχ sο sρednim vesοm early οπyτa 140.8 g). Livestock was kept under standard conditions (temperature 20-22 ° С, humidity 40-60), had the possibility of free-standing

* Take care of food and water a lot. the claimed biologically active 35% of the product is divided into a stylish 0.9-year-old product of two separate forms; the second time on the 9th day of the experiment in - 4 - See 120 mg / kg body weight. An internal living product was introduced with a 0.9% internal water storage unit in a volume of 0.3 ml per live. The study was conducted on 16 days. It’s all over the place

5 Yes, studies were conducted daily study of the weight of animals, their consumption of food and water. After the introduction of the biological product, we studied the effect of it on an invariable system, an inter-vessel system, and on psychological indicators of an operative system.

10 The data received in the course of the experiment (the absence of the death of the laboratory live animals from the introduction of the biologically active product at a dose of 120 mg / kg of the body is not healthy) The preparation causes increased weight gain.

15 laboratory animals who received it as a dose of ^χ 120 mg / kg, and 60 mg / kg of animal weight. Well, after a single administration of a substance at a dose of 60 mg / kg, an increase in weight for 6 subsequent days in experimental animals was $ 70.1 better than in previous ones.

20 days 79.9% more. After the direct introduction of the biological product in the dose of 120 mg / kg, the weight gain in the experimental animals was 38.2% better during the 7th day and 73.3% better than the 8th day. For the entire period of research (16 days) weight loss

25 in the old-fashioned group, he overruled the private group by 50%. The results of studying the dynamics of the consumption of food and water of living animals showed that the single administration of biological drugs to increase the health of the patients

30 average, for 7 days of experience in food by 62.3%, water by

50.6%). After the direct administration of the biological product alive in the dose of 120 mg / kg, the consumption of the body increased slightly. Therefore, the consumption of food for the entire period of research (16 days) increased by only 35 24.3%, that is, the gain in weight was increased by 50% compared to an increase of 24%. The results of the experiment showed that the biologically active drug has a negligible effect on the nerve and heart-vessel system of animals. - 5 - For a better understanding of the present invention, the following methods of obtaining a biological product from the consumer are provided. For example, ι.

5 I stabilized heparin (25 units / ml) at the expense of centrifugation for 15 minutes at a speed of 1,500 rpm, it separates plasma to the initial volume. The mixture is mixed, mixed

You are at the center. The process of washing the variable elements at the factory takes 4-5 times to achieve the success of the agent. Then, the peripheral elements are mixed with a 0.9-percent aqueous solution of a chloride in a ratio of 1.0: 1.0 is compatible and in the case of an ultraviolet

15 mu exposure at a dose of 50,000 J / m ² . The irradiated mixture is centrifuged for 15 minutes, at a speed of 15,000 rpm, separates the hardened liquid and freezes βe. Received 15.0 g of the target product - a biological product in the form of salmon flowers. Prépa-

20 ° C is stored at a temperature of 4 ° C until I year. When using the product, it is dissolved in distilled water and introduced into the body from the calculation of 60 mg / kg of live weight. Example 2. The process is analogous to that described in Example I.

25 At the beginning, the raw materials used are stored, obtained from live animals, and stabilized with a unit of 25 units / ml. Separate peripheral elements are washed out with a buffer solution for the process-7.2, then they are mixed with a buffer system-7.2 at a ratio of 1: 2.0.

30 The resulting suspension is ultraviolet irradiated at a dose of 45,000 J / m ² . Then, the irradiated mixture is centrifuged, separates the hardened liquid and freezes it.

It produces a target product similar to that described in Example I. 35 Example 3.

The biological product of Example I was tested in animals for weight gain in a young child’s body weight. - 6

The biological product was distributed in 350 ml of distilled water and the young man was introduced from the calculation of 60 mg per I kg of live weight. The drug was administered 2 times with an interval of 100 days. The effective period of the drug is 15 days from the last injection. The results of the operation of the appliance were equal to the results obtained from the young team, which introduced a 0.9% external product. The results obtained are given in table I.

10 - Table I

As you can see from the table, the increase in the average weight gain in live weight compared to the experimental one is ^ 6 kg.

Example 4.

15 Biologically active product of Example 2 was tested in tests of weight gain of products. The drug was diluted in distilled water and administered in a dose of 60 mg / kg live weight. The on-line team introduced 0.9 ^ -th aqueous solution on the

rhiya khloorida.

The test results are presented in Table 2.

Τаόлица- 2

1 CONTINUOUS - 0.9 #-th group, ΒΟ LONGEST (18 _% expansion);

2 Experimental Application - 60 mg / kg group biomass (22 ρ active animal) 21.0 33.6 0.57

Deliberate applicability of the presently claimed method of biologically active treatment is used in animal husbandry to increase the weight of animals.

Claims

- 8 - Φ0ΡΜ7LΑ IZΟΕΡΕΤΕΗJAJA.

1. The method of production of a biological product from the tank, including the ultraviolet irradiation of the elements of the battery, which is subject to separation

5 elemenτy, smeshivayuτ and ^χ with izοοsmοτichesκim ρasτvοροm, πο- luchennugo mixture ποdveρgayuτ ulτρaφiοleτοvοmu οblucheniyu with ποsleduyuschim οτdeleniem nadοsadοchnοy zhidκοsτi with ποlu- cheniem tselevοgο προduκτa.

2. Sποsοb πο π.ϊ, ^χ aρaκτeρizuyuschiysya τem, chτο in κa- Yu chesτve izοοsmοτichesκοgο ρasτvορa isποlzuyuτ 0,8-0, 9 ^ -s vοdny ρasτvορ naτρiya χlορida or buφeρny ρasτvορ Τρis-ΗSΙ ρΗ 7,2.

3. The method is simple, which is subject to mixing of the pure elements of the mix with the isostatic mixture.

15 rd in relation to 1,0: 1,0-2,0 are respectively.

4. The method of production, which is subject to the fact that the ultraviolet irradiation of the obtained mixture is obtained after irradiation of 45000-55000 J m ² .

5. The method of manufacture, which is processed by the target 20, is obtained by freeze drying of a suitable liquid.