WO1991018117A1 - Sondes d'arn recombine hybridable replicable et test d'hybridation - Google Patents

Sondes d'arn recombine hybridable replicable et test d'hybridation Download PDF

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Publication number
WO1991018117A1
WO1991018117A1 PCT/US1991/003634 US9103634W WO9118117A1 WO 1991018117 A1 WO1991018117 A1 WO 1991018117A1 US 9103634 W US9103634 W US 9103634W WO 9118117 A1 WO9118117 A1 WO 9118117A1
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WO
WIPO (PCT)
Prior art keywords
rna
recombinant
oligo
interest
polynucleotide
Prior art date
Application number
PCT/US1991/003634
Other languages
English (en)
Inventor
Fred R. Kramer
Paul M. Lizardi
Original Assignee
The Trustees Of Columbia University In The City Of New York
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Trustees Of Columbia University In The City Of New York filed Critical The Trustees Of Columbia University In The City Of New York
Publication of WO1991018117A1 publication Critical patent/WO1991018117A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification

Abstract

Une molécule de sonde d'ARN monocaténaire recombiné replicable et hybridable comprend une séquence de reconnaissance permettant de lier une polymérase d'ARN à correspondance ARN; une séquence requise pour lancer la synthèse de brins du produit au moyen de la polymérase; et une séquence d'ARN hétérologue insérée dans un site spécifique dans la région interne de la molécule recombinée, complémentaire d'un oligonucléotide ou d'un polynucléotide à étudier. Des procédés permettent de déterminer la présence d'une concentration d'un oligonucléotide ou d'un polynucléotide à étudier dans un échantillon et en même temps de déterminer la présence ou la concentration de plusieurs oligonucléotides ou polynucléotides différents à étudier dans un échantillon.
PCT/US1991/003634 1990-05-23 1991-05-23 Sondes d'arn recombine hybridable replicable et test d'hybridation WO1991018117A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52758590A 1990-05-23 1990-05-23
US527,585 1990-05-23

Publications (1)

Publication Number Publication Date
WO1991018117A1 true WO1991018117A1 (fr) 1991-11-28

Family

ID=24102076

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1991/003634 WO1991018117A1 (fr) 1990-05-23 1991-05-23 Sondes d'arn recombine hybridable replicable et test d'hybridation

Country Status (2)

Country Link
AU (1) AU7997791A (fr)
WO (1) WO1991018117A1 (fr)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994009159A2 (fr) * 1992-10-09 1994-04-28 Amoco Corporation Elements d'insertion et acides nucleiques amplifiables
WO1994010335A2 (fr) * 1992-10-09 1994-05-11 Amoco Corporation Methodes d'analyse
WO1996003528A2 (fr) * 1994-07-27 1996-02-08 Cambridge University Technical Services Limited Oligonucleotides et leur utilisation
US5653939A (en) * 1991-11-19 1997-08-05 Massachusetts Institute Of Technology Optical and electrical methods and apparatus for molecule detection
US5780273A (en) * 1993-04-09 1998-07-14 Amoco Corporation Insertion elements and amplifiable nucleic acids
WO2003070982A1 (fr) * 2002-02-22 2003-08-28 Avaris Ab Complexe comportant au moins deux elements biospecifiques separes par un lieur d'acides nucleiques, par exemple pour l'identification de candidats d'administration de medicaments ; bibliotheques combinatoires de tels complexes
US20200332279A1 (en) * 2006-05-31 2020-10-22 Sequenom, Inc. Methods and compositions for the extraction and amplification of nucleic acid from a sample

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4786600A (en) * 1984-05-25 1988-11-22 The Trustees Of Columbia University In The City Of New York Autocatalytic replication of recombinant RNA
WO1990002819A1 (fr) * 1988-09-08 1990-03-22 The Salk Institute For Biological Studies Systemes de detection bases sur l'amplification d'arn replicative
US4957858A (en) * 1986-04-16 1990-09-18 The Salk Instute For Biological Studies Replicative RNA reporter systems

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4786600A (en) * 1984-05-25 1988-11-22 The Trustees Of Columbia University In The City Of New York Autocatalytic replication of recombinant RNA
US4957858A (en) * 1986-04-16 1990-09-18 The Salk Instute For Biological Studies Replicative RNA reporter systems
WO1990002819A1 (fr) * 1988-09-08 1990-03-22 The Salk Institute For Biological Studies Systemes de detection bases sur l'amplification d'arn replicative

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BIOCHEMISTRY, Volume 19, Number 1, issued 1980, MILLS et al., "Modification of Cytidines in a QB Replicase Template: Analysis of Conformation and Localization of Lethal Nucleotide Substitutions", pages 228-236. *
GENE, Volume 36, issued 1985, LANGDALE et al., "A Rapid Method of Gene Detection Using DNA Bound to Sephacryl", pages 201-210. *
GENE, Volume 61, issued 1987, URDEA et al., "A Novel Method for the Rapid Detection of Specific Nucleotide Sequences in Crude Biological Samples without Blotting of Radioactivity: Application to the Analysis of Hepatitis B Virus in Human Serum", pages 253-264. *
JOURNAL OF BIOCHEMISTRY, Volume 93, Number 3, issued 1983, NISHIHARA et al., "Localization of the QB Replicase Recognition Site in MDV-1 RNA", pages 669-674. *
NUCLEIC ACIDS RESEARCH, Volume 14, Number 14, issued 1986, CHU et al., "Synthesis of an Amplifiable Reporter RNA for Bioassays", pages 5591-5603. *
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES (USA), Volume 75, Number 11, issued November 1978, KRAMER et al., "RNA Sequencing with Radioactive Chain Terminating Ribonucleotides", pages 5334-5338. *
THE JOURNAL OF BIOLOGICAL CHEMISTRY, Volume 258, Number 3, issued 10 February 1983, BAUSCH et al., "Terminal Adenylation in the Synthesis of RNA by QB Replicase", pages 1978-1984. *

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5653939A (en) * 1991-11-19 1997-08-05 Massachusetts Institute Of Technology Optical and electrical methods and apparatus for molecule detection
US5846708A (en) * 1991-11-19 1998-12-08 Massachusetts Institiute Of Technology Optical and electrical methods and apparatus for molecule detection
WO1994010335A3 (fr) * 1992-10-09 1994-08-18 Amoco Corp Methodes d'analyse
WO1994009159A2 (fr) * 1992-10-09 1994-04-28 Amoco Corporation Elements d'insertion et acides nucleiques amplifiables
WO1994009159A3 (fr) * 1992-10-09 1994-06-09 Amoco Corp Elements d'insertion et acides nucleiques amplifiables
WO1994010335A2 (fr) * 1992-10-09 1994-05-11 Amoco Corporation Methodes d'analyse
US5780273A (en) * 1993-04-09 1998-07-14 Amoco Corporation Insertion elements and amplifiable nucleic acids
WO1996003528A2 (fr) * 1994-07-27 1996-02-08 Cambridge University Technical Services Limited Oligonucleotides et leur utilisation
WO1996003528A3 (fr) * 1994-07-27 1996-04-18 Lynxvale Ltd Oligonucleotides et leur utilisation
WO2003070982A1 (fr) * 2002-02-22 2003-08-28 Avaris Ab Complexe comportant au moins deux elements biospecifiques separes par un lieur d'acides nucleiques, par exemple pour l'identification de candidats d'administration de medicaments ; bibliotheques combinatoires de tels complexes
US7824853B2 (en) 2002-02-22 2010-11-02 Avaris Ab Complex comprising at least two biospecific elements separated by a nucleic acid linker e.g. for identification of drug delivery candidates combinatorial library of such complexes
US20200332279A1 (en) * 2006-05-31 2020-10-22 Sequenom, Inc. Methods and compositions for the extraction and amplification of nucleic acid from a sample
US11952569B2 (en) * 2006-05-31 2024-04-09 Sequenom, Inc. Methods and compositions for the extraction and amplification of nucleic acid from a sample

Also Published As

Publication number Publication date
AU7997791A (en) 1991-12-10

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